TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Santrač, Isidora
AU  - Žižić, Milan
AU  - AQUILANTI, Giuliana
AU  - Gianoncelli, Alessandra
AU  - Bonanni, Valentina
AU  - Danilović Luković, Jelena
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3139
AB  - Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.
C3  - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
T1  - The coordination and storage of manganese in the microalga Haematococcus pluvialis
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3139
ER  - 

@conference{
author = "Santrač, Isidora and Žižić, Milan and AQUILANTI, Giuliana and Gianoncelli, Alessandra and Bonanni, Valentina and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Spasojević, Ivan",
year = "2023",
abstract = "Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.",
journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia",
title = "The coordination and storage of manganese in the microalga Haematococcus pluvialis",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3139"
}

Santrač, I., Žižić, M., AQUILANTI, G., Gianoncelli, A., Bonanni, V., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S.,& Spasojević, I.. (2023). The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia, 45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139

Santrač I, Žižić M, AQUILANTI G, Gianoncelli A, Bonanni V, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Spasojević I. The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

Santrač, Isidora, Žižić, Milan, AQUILANTI, Giuliana, Gianoncelli, Alessandra, Bonanni, Valentina, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Spasojević, Ivan, "The coordination and storage of manganese in the microalga Haematococcus pluvialis" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):45,
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

TY  - JOUR
AU  - Luzajic Bozinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Nikolić, Anja
AU  - Radovanović, Anita
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Vasić, Mirjana
AU  - Milošević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2453
AB  - Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.
PB  - Walter de Gruyter {GmbH}
T2  - Acta Veterinaria-Beograd
T1  - Maternal Subclinical and Clinical Hypothyroidism Effects on Rat Offspring: A Story of the Skin and its Derivatives
EP  - 248
IS  - 2
SP  - 231
VL  - 73
DO  - 10.2478/acve-2023-0018
ER  - 

@article{
author = "Luzajic Bozinovski, Tijana and Danilović Luković, Jelena and Nikolić, Anja and Radovanović, Anita and Marković, Danica and Kovačević Filipović, Milica and Vasić, Mirjana and Milošević, Ivan",
year = "2023",
abstract = "Epidermis stem cells have a crucial role through the processes of proliferation and
differentiation, to replace cells that are constantly lost during tissue turnover or
following injury. On the other hand, thyroid hormones regulate the proliferation and
differentiation of epidermal cells and thus significantly influence the homeostasis
of the skin. It is well known that maternal hypothyroidism during pregnancy leads
to impaired development of many organ systems in their offspring. However, there
is a lack of data about the influence of maternal subclinical hypothyroidism during
pregnancy and lactation on the development of the skin and its derivatives in the litter.
The aim of this study was to investigate the effects of maternal thyroid dysfunction on
the development of the skin and its derivatives in their offspring in the early postnatal
period. Antithyroid substance 6-n-propyl-2-thiouracil was added into the drinking water
to female Albino Oxfords rats from the beginning of pregnancy and during lactation,
with the aim to induce subclinical and overt form of hypothyroidism. Skin samples
were taken from male pups within twenty-four hours and seven days after birth. The
main findings of this investigation were that both forms of maternal hypothyroidism
lead to serious damage of the epidermis in pups in terms of pronounced hyperkeratosis
and reduction of the germinal layer along with a reduced number of hair follicles and
their delayed morphogenesis. Epidermal impairments were more pronounced in pups
with the overt form of hypothyroidism while offspring with the subclinical form had
impairments that were less pronounced and delayed in occurrence.",
publisher = "Walter de Gruyter {GmbH}",
journal = "Acta Veterinaria-Beograd",
title = "Maternal Subclinical and Clinical Hypothyroidism Effects on Rat Offspring: A Story of the Skin and its Derivatives",
pages = "248-231",
number = "2",
volume = "73",
doi = "10.2478/acve-2023-0018"
}

Luzajic Bozinovski, T., Danilović Luković, J., Nikolić, A., Radovanović, A., Marković, D., Kovačević Filipović, M., Vasić, M.,& Milošević, I.. (2023). Maternal Subclinical and Clinical Hypothyroidism Effects on Rat Offspring: A Story of the Skin and its Derivatives. in Acta Veterinaria-Beograd
Walter de Gruyter {GmbH}., 73(2), 231-248.
https://doi.org/10.2478/acve-2023-0018

Luzajic Bozinovski T, Danilović Luković J, Nikolić A, Radovanović A, Marković D, Kovačević Filipović M, Vasić M, Milošević I. Maternal Subclinical and Clinical Hypothyroidism Effects on Rat Offspring: A Story of the Skin and its Derivatives. in Acta Veterinaria-Beograd. 2023;73(2):231-248.
doi:10.2478/acve-2023-0018 .

Luzajic Bozinovski, Tijana, Danilović Luković, Jelena, Nikolić, Anja, Radovanović, Anita, Marković, Danica, Kovačević Filipović, Milica, Vasić, Mirjana, Milošević, Ivan, "Maternal Subclinical and Clinical Hypothyroidism Effects on Rat Offspring: A Story of the Skin and its Derivatives" in Acta Veterinaria-Beograd, 73, no. 2 (2023):231-248,
https://doi.org/10.2478/acve-2023-0018 . .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Janković, Tamara
AU  - Goč, Sanja
AU  - Janjić, Filip
AU  - Mitic, Ninoslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2732
AB  - Glycans, complex carbohydrates bound to lipids and proteins, play important roles in biological processes.
Prostasomes, extracellular vesicles from prostate epithelial cells, have a glycan composition influenced by
their cellular origin and by the composition of the seminal plasma into which they are secreted. We
hypothesized that the membrane-associated glycans could be used as selective targets for separation of
prostasomes and as relevant parameter for distinction of their populations.
Prostasomes from seminal plasma of normozoospermic and oligozoospermic men were separated by
ion-exchange- and lectin-affinity chromatography using concanavalin A (ConA) lectin, specific for
mannosylated structures, and wheat germ agglutinin (WGA), specific for sialic acid. The presence of
tetraspanins (CD63, CD9 and CD81), galectin-3 (gal-3) and gamma-glutamyl transferase (GGT) as known
vesicle markers were monitored in association with distinct glycans. Their distribution was also analysed upon
treatment of prostasomes with non-ionic detergent.
Membrane-associated GGT in the context of Con A- and WGA-reactive glycans mark prostasome
populations from normozoospermic and oligozoospermic men. The assembly of tetraspanins, gal-3, and
distinct N-glycans defines the solubilisation signature of prostasomes. WGA-reactive glycoproteins
co-localize with CD9 and gal-3 in detergent-resistant domains, whereas ConA-reactive glycoproteins were
distributed in detergent-sensitive domains along with CD63 and GGT. Subtle differences in the
composition/presentation of examined molecules made difference among vesicles sharing the same physical properties in each group as well as between them.
The results obtained suggest the potential of glyco-parameters as reference markers for EVs populations.
PB  - Medical University Graz, Austria
C3  - Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria
T1  - Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes
EP  - 52
SP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2732
ER  - 

@conference{
author = "Danilović Luković, Jelena and Janković, Tamara and Goč, Sanja and Janjić, Filip and Mitic, Ninoslav",
year = "2023",
abstract = "Glycans, complex carbohydrates bound to lipids and proteins, play important roles in biological processes.
Prostasomes, extracellular vesicles from prostate epithelial cells, have a glycan composition influenced by
their cellular origin and by the composition of the seminal plasma into which they are secreted. We
hypothesized that the membrane-associated glycans could be used as selective targets for separation of
prostasomes and as relevant parameter for distinction of their populations.
Prostasomes from seminal plasma of normozoospermic and oligozoospermic men were separated by
ion-exchange- and lectin-affinity chromatography using concanavalin A (ConA) lectin, specific for
mannosylated structures, and wheat germ agglutinin (WGA), specific for sialic acid. The presence of
tetraspanins (CD63, CD9 and CD81), galectin-3 (gal-3) and gamma-glutamyl transferase (GGT) as known
vesicle markers were monitored in association with distinct glycans. Their distribution was also analysed upon
treatment of prostasomes with non-ionic detergent.
Membrane-associated GGT in the context of Con A- and WGA-reactive glycans mark prostasome
populations from normozoospermic and oligozoospermic men. The assembly of tetraspanins, gal-3, and
distinct N-glycans defines the solubilisation signature of prostasomes. WGA-reactive glycoproteins
co-localize with CD9 and gal-3 in detergent-resistant domains, whereas ConA-reactive glycoproteins were
distributed in detergent-sensitive domains along with CD63 and GGT. Subtle differences in the
composition/presentation of examined molecules made difference among vesicles sharing the same physical properties in each group as well as between them.
The results obtained suggest the potential of glyco-parameters as reference markers for EVs populations.",
publisher = "Medical University Graz, Austria",
journal = "Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria",
title = "Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2732"
}

Danilović Luković, J., Janković, T., Goč, S., Janjić, F.,& Mitic, N.. (2023). Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes. in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria
Medical University Graz, Austria., 52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2732

Danilović Luković J, Janković T, Goč S, Janjić F, Mitic N. Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes. in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria. 2023;:52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2732 .

Danilović Luković, Jelena, Janković, Tamara, Goč, Sanja, Janjić, Filip, Mitic, Ninoslav, "Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes" in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria (2023):52-52,
https://hdl.handle.net/21.15107/rcub_rimsi_2732 .

TY  - JOUR
AU  - Janković, Tamara
AU  - Danilović Luković, Jelena
AU  - Goč, Sanja
AU  - Mitić, Ninoslav
AU  - Hajduković, Ljiljana
AU  - Janković, Miroslava
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2497
AB  - Background. Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration
and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely
explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained
on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of
selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between
known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should
contribute to define them together as possible multimarkers.
Methods. GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed
using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A).
Results. Widely distributed GGT (with two to three partially resolved peaks), which may correspond to high molecular
mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and
small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive
glycans, as well as mucin-associated antigens CA19-9 and CA125.
Conclusions. GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle
fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated
glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms
of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT.
PB  - Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic
T2  - Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
T1  - Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity
DO  - 10.5507/bp.2023.031
ER  - 

@article{
author = "Janković, Tamara and Danilović Luković, Jelena and Goč, Sanja and Mitić, Ninoslav and Hajduković, Ljiljana and Janković, Miroslava",
year = "2023",
abstract = "Background. Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration
and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely
explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained
on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of
selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between
known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should
contribute to define them together as possible multimarkers.
Methods. GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed
using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A).
Results. Widely distributed GGT (with two to three partially resolved peaks), which may correspond to high molecular
mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and
small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive
glycans, as well as mucin-associated antigens CA19-9 and CA125.
Conclusions. GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle
fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated
glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms
of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT.",
publisher = "Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic",
journal = "Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia",
title = "Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity",
doi = "10.5507/bp.2023.031"
}

Janković, T., Danilović Luković, J., Goč, S., Mitić, N., Hajduković, L.,& Janković, M.. (2023). Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity. in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia
Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic..
https://doi.org/10.5507/bp.2023.031

Janković T, Danilović Luković J, Goč S, Mitić N, Hajduković L, Janković M. Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity. in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia. 2023;.
doi:10.5507/bp.2023.031 .

Janković, Tamara, Danilović Luković, Jelena, Goč, Sanja, Mitić, Ninoslav, Hajduković, Ljiljana, Janković, Miroslava, "Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity" in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia (2023),
https://doi.org/10.5507/bp.2023.031 . .

TY  - JOUR
AU  - Danilović Luković, Jelena
AU  - Goč, Sanja
AU  - Mitic, Ninoslav
AU  - Janković, Tamara
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2476
AB  - The carbohydrate portion of proteins and lipids mediates a variety of biological processes. Revealing their
underlying principles is a challenging task that could contribute to a better understanding of many patho/physiological
conditions. On the other hand, the interest in extracellular vesicles (EVs) has increased in recent years due to their
involvement in intercellular communication leading to an array of functional and structural changes in recipient cells.
Their characterization uncovered an exceptional diversity in size, morphology, as well as in membrane and cargo content.
Monitoring/analysis of surface glycosylation of EVs originating from the prostate, termed prostasomes, revealed
their substantial contribution to the complexity of seminal plasma (SP) glycome. Heterogeneity of surface glycans confirm
the existence of several prostasome subpopulations. Presentation of surface glycans on prostasomal membrane is
strongly affected by co-localized membrane-associated glycoproteins and tetraspanins. They appear to be organized in
established/regular distribution patterns on membrane domains. Surface glycans are a component of EVs membrane
that affects its functionality and potentially a distinction marker of prostasome subpopulations. Further understanding
of the complex composition of glycans on EVs might explain the relation of their structure with functional alterations
in distinct patho/physiological conditions.
PB  - Faculty of Sciences, Department of Biology, University of Novi Sad, Serbia
T2  - Biologia Serbica
T1  - Extracellular vesicles in a maze of glycomic complexity
EP  - 32
IS  - 1
SP  - 25
VL  - 44
DO  - 10.5281/zenodo.7075050
ER  - 

@article{
author = "Danilović Luković, Jelena and Goč, Sanja and Mitic, Ninoslav and Janković, Tamara",
year = "2022",
abstract = "The carbohydrate portion of proteins and lipids mediates a variety of biological processes. Revealing their
underlying principles is a challenging task that could contribute to a better understanding of many patho/physiological
conditions. On the other hand, the interest in extracellular vesicles (EVs) has increased in recent years due to their
involvement in intercellular communication leading to an array of functional and structural changes in recipient cells.
Their characterization uncovered an exceptional diversity in size, morphology, as well as in membrane and cargo content.
Monitoring/analysis of surface glycosylation of EVs originating from the prostate, termed prostasomes, revealed
their substantial contribution to the complexity of seminal plasma (SP) glycome. Heterogeneity of surface glycans confirm
the existence of several prostasome subpopulations. Presentation of surface glycans on prostasomal membrane is
strongly affected by co-localized membrane-associated glycoproteins and tetraspanins. They appear to be organized in
established/regular distribution patterns on membrane domains. Surface glycans are a component of EVs membrane
that affects its functionality and potentially a distinction marker of prostasome subpopulations. Further understanding
of the complex composition of glycans on EVs might explain the relation of their structure with functional alterations
in distinct patho/physiological conditions.",
publisher = "Faculty of Sciences, Department of Biology, University of Novi Sad, Serbia",
journal = "Biologia Serbica",
title = "Extracellular vesicles in a maze of glycomic complexity",
pages = "32-25",
number = "1",
volume = "44",
doi = "10.5281/zenodo.7075050"
}

Danilović Luković, J., Goč, S., Mitic, N.,& Janković, T.. (2022). Extracellular vesicles in a maze of glycomic complexity. in Biologia Serbica
Faculty of Sciences, Department of Biology, University of Novi Sad, Serbia., 44(1), 25-32.
https://doi.org/10.5281/zenodo.7075050

Danilović Luković J, Goč S, Mitic N, Janković T. Extracellular vesicles in a maze of glycomic complexity. in Biologia Serbica. 2022;44(1):25-32.
doi:10.5281/zenodo.7075050 .

Danilović Luković, Jelena, Goč, Sanja, Mitic, Ninoslav, Janković, Tamara, "Extracellular vesicles in a maze of glycomic complexity" in Biologia Serbica, 44, no. 1 (2022):25-32,
https://doi.org/10.5281/zenodo.7075050 . .

TY  - CONF
AU  - Goč, Sanja
AU  - Mitic, Ninoslav
AU  - Janković, Tamara
AU  - Danilović Luković, Jelena
AU  - Janković, Miroslava
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2800
AB  - Простазоми су екстраћелијске везикуле (EVs) које епителијалне ћелије простате секретују у семену течност. Они имају двослојну липидну мембрану и садрже компоненте ћелија које их излучују (протеинe, фрагментe ДНK, информациону РНK, микрo РНK, дугу некодирaјућу РНK, липидe и метаболитe). Циљ овог рада је био да се анализом композициje и обрасца дистрибуције манозилованих/сијалинизованих N-гликана и маркера EVs: тетраспанина (CD9, CD63, CD81), гама-глутамил-трансферазе (GGT) и галектина-3 (gal-3), утврди јединствени гликански састав мембране простазома као нови референтни маркер. Простазоми су изоловани из семених плазми мушкараца са нормоспермијом и олигоспермијом. Дистрибуција N-гликана, тетраспанина, GGT и gal-3 на површини мембране простазома је добијена анализом солубилизованих простазома. N-гликани су детектовани на оснoву реактивности са лектинима: Wheat germ agglutinin (WGA) и Concanavalin A (ConA), а тетраспанини и gal-3 имуно-дотблот-ом. GGT је праћенa мерењем ензимске активности. Установљена су два обрасца дистрибуције код обе испитиване групе: CD9/gal-3/WGA-реактивни гликопротеини и CD63/GGT/ConA-реактивни гликопротеини. Показано је да постоји сличност у дистрибуцији N-гликана и GGT, док су разлике уочене у дистрибуцији тетраспанина и gal-3. Разлике у композицији и дистрибуцији гликана на површини мембране простазома могле би представљати потенцијални индикатор промена у репродуктивној физиологији.
PB  - Српско биолошко друштво
C3  - Treci kongres biologa Srbije
T1  - Kompozicija i distribucija glikana na membrani prostazoma kod muškaraca sa normospermijom i oligospermijom
EP  - 328
SP  - 328
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2800
ER  - 

@conference{
author = "Goč, Sanja and Mitic, Ninoslav and Janković, Tamara and Danilović Luković, Jelena and Janković, Miroslava",
year = "2022",
abstract = "Простазоми су екстраћелијске везикуле (EVs) које епителијалне ћелије простате секретују у семену течност. Они имају двослојну липидну мембрану и садрже компоненте ћелија које их излучују (протеинe, фрагментe ДНK, информациону РНK, микрo РНK, дугу некодирaјућу РНK, липидe и метаболитe). Циљ овог рада је био да се анализом композициje и обрасца дистрибуције манозилованих/сијалинизованих N-гликана и маркера EVs: тетраспанина (CD9, CD63, CD81), гама-глутамил-трансферазе (GGT) и галектина-3 (gal-3), утврди јединствени гликански састав мембране простазома као нови референтни маркер. Простазоми су изоловани из семених плазми мушкараца са нормоспермијом и олигоспермијом. Дистрибуција N-гликана, тетраспанина, GGT и gal-3 на површини мембране простазома је добијена анализом солубилизованих простазома. N-гликани су детектовани на оснoву реактивности са лектинима: Wheat germ agglutinin (WGA) и Concanavalin A (ConA), а тетраспанини и gal-3 имуно-дотблот-ом. GGT је праћенa мерењем ензимске активности. Установљена су два обрасца дистрибуције код обе испитиване групе: CD9/gal-3/WGA-реактивни гликопротеини и CD63/GGT/ConA-реактивни гликопротеини. Показано је да постоји сличност у дистрибуцији N-гликана и GGT, док су разлике уочене у дистрибуцији тетраспанина и gal-3. Разлике у композицији и дистрибуцији гликана на површини мембране простазома могле би представљати потенцијални индикатор промена у репродуктивној физиологији.",
publisher = "Српско биолошко друштво",
journal = "Treci kongres biologa Srbije",
title = "Kompozicija i distribucija glikana na membrani prostazoma kod muškaraca sa normospermijom i oligospermijom",
pages = "328-328",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2800"
}

Goč, S., Mitic, N., Janković, T., Danilović Luković, J.,& Janković, M.. (2022). Kompozicija i distribucija glikana na membrani prostazoma kod muškaraca sa normospermijom i oligospermijom. in Treci kongres biologa Srbije
Српско биолошко друштво., 328-328.
https://hdl.handle.net/21.15107/rcub_rimsi_2800

Goč S, Mitic N, Janković T, Danilović Luković J, Janković M. Kompozicija i distribucija glikana na membrani prostazoma kod muškaraca sa normospermijom i oligospermijom. in Treci kongres biologa Srbije. 2022;:328-328.
https://hdl.handle.net/21.15107/rcub_rimsi_2800 .

Goč, Sanja, Mitic, Ninoslav, Janković, Tamara, Danilović Luković, Jelena, Janković, Miroslava, "Kompozicija i distribucija glikana na membrani prostazoma kod muškaraca sa normospermijom i oligospermijom" in Treci kongres biologa Srbije (2022):328-328,
https://hdl.handle.net/21.15107/rcub_rimsi_2800 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Danilović Luković, Jelena
AU  - Milivojević, Marija
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2403
AB  - METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana
EP  - 748
SP  - 747
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2403
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Danilović Luković, Jelena and Milivojević, Marija and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana",
pages = "748-747",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2403"
}

Vojvodić, S., Stanić, M., Danilović Luković, J., Milivojević, M., Zechmann, B., Dimitrijević, M., Opačić, M., Pittman, J. K.,& Spasojević, I.. (2022). Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403

Vojvodić S, Stanić M, Danilović Luković J, Milivojević M, Zechmann B, Dimitrijević M, Opačić M, Pittman JK, Spasojević I. Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

Vojvodić, Snežana, Stanić, Marina, Danilović Luković, Jelena, Milivojević, Marija, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Pittman, Jon K., Spasojević, Ivan, "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):747-748,
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

TY  - CONF
AU  - Milošević, Ivan
AU  - Luzajic Bozinovski, Tijana
AU  - Danilović Luković, Jelena
AU  - Gajdov, Vladimir
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2609
AB  - Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from subclinical and overt hypothyroid does, in their early postnatal development.
Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was performed by counting the number of positive dots in chondrocytes.
The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation. We conclude that both forms of maternal hypothyroidism in rats lead to accelerated proliferation, slowed autophagy and compromised apoptosis of terminal hypertrophic chondrocytes in the early infantile period and a delay in cartilage to bone transition.
PB  - Faculty of Veterinary Medicine, University of Zagreb, Croatia
C3  - 9th International Conference Veterinary Science and Profession 2021, Zagreb
T1  - Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups
EP  - 114
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2609
ER  - 

@conference{
author = "Milošević, Ivan and Luzajic Bozinovski, Tijana and Danilović Luković, Jelena and Gajdov, Vladimir and Marković, Danica and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2021",
abstract = "Overt and subclinical maternal hypothyroidism affects cartilage and bone extracellular matrix formation during fetal endochondral bone development. Less is known about the effect maternal hypothyroidism has on cell proliferation and death of growth plate chondrocytes.
This study aimed to evaluate the expression of proliferation (Ki67), autophagy (LC3) and apoptosis (caspase 3) markers in the epiphyseal cartilage of the tibia in pups from subclinical and overt hypothyroid does, in their early postnatal development.
Hypothyroidism was induced with propylthiouracil using low (1.5 mg/L) for subclinical (H1) and high dose (150 mg/L) for overt (H2) form. Propylthiouracil was administered through drinking water in pregnant Albino Oxford does from the first day of gravidity and during lactation. Control (C) group was not treated. Six, seven-day-old male pups from each group were euthanized. Histological examinations were performed on paraffin sections of the proximal tibial growth plate. Immunohistochemistry was used to assess the expression of Ki67, LC3 and caspase 3. The number of Ki67 positive chondrocytes was evaluated in the resting and proliferating zone using Image J. The expression of LC3 was performed by counting the number of positive dots in chondrocytes.
The number of Ki67 positive chondrocytes was higher in both hypothyroid groups, while the number of LC3 positive dots per cell was lower indicating that the chondrocyte cell cycle in hypothyroid animals is faster and that the basal level of autophagy is reduced due to frequent mitosis. Compared to controls, caspase 3 expression in the terminal hypertrophic chondrocytes was reduced in H1 and completely absent in H2 pups which might indicate impaired differentiation. We conclude that both forms of maternal hypothyroidism in rats lead to accelerated proliferation, slowed autophagy and compromised apoptosis of terminal hypertrophic chondrocytes in the early infantile period and a delay in cartilage to bone transition.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb, Croatia",
journal = "9th International Conference Veterinary Science and Profession 2021, Zagreb",
title = "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups",
pages = "114-114",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2609"
}

Milošević, I., Luzajic Bozinovski, T., Danilović Luković, J., Gajdov, V., Marković, D., Kovačević Filipović, M.,& Radovanovic, A.. (2021). Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb
Faculty of Veterinary Medicine, University of Zagreb, Croatia., 114-114.
https://hdl.handle.net/21.15107/rcub_rimsi_2609

Milošević I, Luzajic Bozinovski T, Danilović Luković J, Gajdov V, Marković D, Kovačević Filipović M, Radovanovic A. Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups. in 9th International Conference Veterinary Science and Profession 2021, Zagreb. 2021;:114-114.
https://hdl.handle.net/21.15107/rcub_rimsi_2609 .

Milošević, Ivan, Luzajic Bozinovski, Tijana, Danilović Luković, Jelena, Gajdov, Vladimir, Marković, Danica, Kovačević Filipović, Milica, Radovanovic, Anita, "Hypothyroidism in pregnant rats affects proliferation and cell death of growth plate chondrocytes in pups" in 9th International Conference Veterinary Science and Profession 2021, Zagreb (2021):114-114,
https://hdl.handle.net/21.15107/rcub_rimsi_2609 .

TY  - JOUR
AU  - Janković, Tamara
AU  - Danilović Luković, Jelena
AU  - Miler, Irena
AU  - Mitic, Ninoslav
AU  - Hajduković, Ljiljana
AU  - Janković, Miroslava
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2464
AB  - Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct
tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of
different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal
proteins were determined aiming at the solubilization signature as an intrinsic multimolecular
marker and a new parameter suitable as a reference for the comparison of EVs populations in health and
disease.
Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that
maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase
(GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission
electron microscopy, electrophoresis, and lectin blot.
Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count
revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly
part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral
membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns
mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/
GGT/concanavalin A-reactive glycoproteins.
Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the
surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular
environment, both of which contribute to the composition of spatial membrane arrangements.
PB  - Upsala Medical Society
T2  - Upsala journal of medical sciences
T1  - Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men
VL  - 126
DO  - 10.48101/ujms.v126.7673
ER  - 

@article{
author = "Janković, Tamara and Danilović Luković, Jelena and Miler, Irena and Mitic, Ninoslav and Hajduković, Ljiljana and Janković, Miroslava",
year = "2021",
abstract = "Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct
tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of
different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal
proteins were determined aiming at the solubilization signature as an intrinsic multimolecular
marker and a new parameter suitable as a reference for the comparison of EVs populations in health and
disease.
Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that
maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase
(GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission
electron microscopy, electrophoresis, and lectin blot.
Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count
revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly
part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral
membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns
mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/
GGT/concanavalin A-reactive glycoproteins.
Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the
surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular
environment, both of which contribute to the composition of spatial membrane arrangements.",
publisher = "Upsala Medical Society",
journal = "Upsala journal of medical sciences",
title = "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men",
volume = "126",
doi = "10.48101/ujms.v126.7673"
}

Janković, T., Danilović Luković, J., Miler, I., Mitic, N., Hajduković, L.,& Janković, M.. (2021). Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences
Upsala Medical Society., 126.
https://doi.org/10.48101/ujms.v126.7673

Janković T, Danilović Luković J, Miler I, Mitic N, Hajduković L, Janković M. Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences. 2021;126.
doi:10.48101/ujms.v126.7673 .

Janković, Tamara, Danilović Luković, Jelena, Miler, Irena, Mitic, Ninoslav, Hajduković, Ljiljana, Janković, Miroslava, "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men" in Upsala journal of medical sciences, 126 (2021),
https://doi.org/10.48101/ujms.v126.7673 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2420
AB  - Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.
PB  - Elsevier
PB  - Society for Free Radical Research Europe (SFRR-E)
PB  - Ministry of Education, Science and Technological Development (Republic of Serbia)
PB  - Oxygen Club of California
C3  - Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
T1  - Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana
EP  - 145
SP  - 145
DO  - 10.1016/j.freeradbiomed.2021.08.148
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Danilović Luković, Jelena and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2021",
abstract = "Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.",
publisher = "Elsevier, Society for Free Radical Research Europe (SFRR-E), Ministry of Education, Science and Technological Development (Republic of Serbia), Oxygen Club of California",
journal = "Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts",
title = "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana",
pages = "145-145",
doi = "10.1016/j.freeradbiomed.2021.08.148"
}

Vojvodić, S., Stanić, M., Zechmann, B., Dimitrijević, M., Opačić, M., Danilović Luković, J., Morina, A., Pittman, J. K.,& Spasojević, I.. (2021). Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
Elsevier., 145-145.
https://doi.org/10.1016/j.freeradbiomed.2021.08.148

Vojvodić S, Stanić M, Zechmann B, Dimitrijević M, Opačić M, Danilović Luković J, Morina A, Pittman JK, Spasojević I. Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts. 2021;:145-145.
doi:10.1016/j.freeradbiomed.2021.08.148 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Danilović Luković, Jelena, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana" in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts (2021):145-145,
https://doi.org/10.1016/j.freeradbiomed.2021.08.148 . .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Milić, Jelena
AU  - Stanić, Marina
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2392
AB  - Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - The effect of increased nickel concentrations on Chlorella sorokiniana culture
EP  - 58
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2392
ER  - 

@conference{
author = "Dimitrijević, Milena and Vojvodić, Snežana and Opačić, Miloš and Danilović Luković, Jelena and Milić, Jelena and Stanić, Marina and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "The effect of increased nickel concentrations on Chlorella sorokiniana culture",
pages = "58-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2392"
}

Dimitrijević, M., Vojvodić, S., Opačić, M., Danilović Luković, J., Milić, J., Stanić, M.,& Spasojević, I.. (2021). The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392

Dimitrijević M, Vojvodić S, Opačić M, Danilović Luković J, Milić J, Stanić M, Spasojević I. The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

Dimitrijević, Milena, Vojvodić, Snežana, Opačić, Miloš, Danilović Luković, Jelena, Milić, Jelena, Stanić, Marina, Spasojević, Ivan, "The effect of increased nickel concentrations on Chlorella sorokiniana culture" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):57-58,
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Danilović Luković, Jelena
AU  - Korac, Aleksandra
AU  - Stanić, Marina
AU  - Spasić, Slađana
AU  - Galland, Paul
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1399
AB  - The growing zone (GZ) of the unicellular coenocytic sporangiophore of Phycomyces blakesleeanus represents the site of stimulus reception (light, gravity, gas) and stimulus response, i.e., local modulations of the elongation growth, which may result, in dependence of the stimulus direction, in tropic bending. Until now, evidence for a possible participation of the columella in sensory reception is absent. We confirm with light microscopy earlier studies that show that the GZ and the columella are not separated by a membrane or cell wall, but rather form a spatial continuum that allows free exchange of cytoplasm and organelle transport. Evidence is presented that the columella is responsive to external stimuli. Columellae, from which spores and sporangial cell wall had been removed, respond to exogenous auxin with a local depolarization of the membrane potential and an increased growth rate of the GZ. In contrast, auxin applied to the GZ causes a decrease of the growth rate irrespective of the presence or absence of sporangia. The response pattern is specific and relevant for the sensory reception of Phycomyces, because the light-insensitive mutant C148carAmadC, which lacks the RAS-GAP protein MADC, displays abnormal IAA sensitivity and membrane depolarization. We argue that the traditional concept of the GZ as the only stimulus-sensitive zone should be abandoned in favor of a model in which GZ and columella operate as a single entity capable to orchestrate a multitude of stimulus inputs, including auxin, to modulate the membrane potential and elongation growth of the GZ.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential
DO  - 10.1007/s00709-021-01709-y
ER  - 

@article{
author = "Živanović, Branka  D. and Danilović Luković, Jelena and Korac, Aleksandra and Stanić, Marina and Spasić, Slađana and Galland, Paul",
year = "2021",
abstract = "The growing zone (GZ) of the unicellular coenocytic sporangiophore of Phycomyces blakesleeanus represents the site of stimulus reception (light, gravity, gas) and stimulus response, i.e., local modulations of the elongation growth, which may result, in dependence of the stimulus direction, in tropic bending. Until now, evidence for a possible participation of the columella in sensory reception is absent. We confirm with light microscopy earlier studies that show that the GZ and the columella are not separated by a membrane or cell wall, but rather form a spatial continuum that allows free exchange of cytoplasm and organelle transport. Evidence is presented that the columella is responsive to external stimuli. Columellae, from which spores and sporangial cell wall had been removed, respond to exogenous auxin with a local depolarization of the membrane potential and an increased growth rate of the GZ. In contrast, auxin applied to the GZ causes a decrease of the growth rate irrespective of the presence or absence of sporangia. The response pattern is specific and relevant for the sensory reception of Phycomyces, because the light-insensitive mutant C148carAmadC, which lacks the RAS-GAP protein MADC, displays abnormal IAA sensitivity and membrane depolarization. We argue that the traditional concept of the GZ as the only stimulus-sensitive zone should be abandoned in favor of a model in which GZ and columella operate as a single entity capable to orchestrate a multitude of stimulus inputs, including auxin, to modulate the membrane potential and elongation growth of the GZ.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential",
doi = "10.1007/s00709-021-01709-y"
}

Živanović, Branka  D., Danilović Luković, J., Korac, A., Stanić, M., Spasić, S.,& Galland, P.. (2021). Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential. in Protoplasma
Springer Wien, Wien..
https://doi.org/10.1007/s00709-021-01709-y

Živanović, Branka  D., Danilović Luković J, Korac A, Stanić M, Spasić S, Galland P. Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential. in Protoplasma. 2021;.
doi:10.1007/s00709-021-01709-y .

Živanović, Branka  D., Danilović Luković, Jelena, Korac, Aleksandra, Stanić, Marina, Spasić, Slađana, Galland, Paul, "Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential" in Protoplasma (2021),
https://doi.org/10.1007/s00709-021-01709-y . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Jevtović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Lizzul, Alessandro Marco
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1371
AB  - The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana
VL  - 260
DO  - 10.1016/j.chemosphere.2020.127553
ER  - 

@article{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Jevtović, Mima and Bogdanović Pristov, Jelena and Stanić, Marina and Lizzul, Alessandro Marco and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana",
volume = "260",
doi = "10.1016/j.chemosphere.2020.127553"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Jevtović, M., Bogdanović Pristov, J., Stanić, M., Lizzul, A. M., Pittman, J. K.,& Spasojević, I.. (2020). The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 260.
https://doi.org/10.1016/j.chemosphere.2020.127553

Vojvodić S, Danilović Luković J, Zechmann B, Jevtović M, Bogdanović Pristov J, Stanić M, Lizzul AM, Pittman JK, Spasojević I. The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere. 2020;260.
doi:10.1016/j.chemosphere.2020.127553 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Jevtović, Mima, Bogdanović Pristov, Jelena, Stanić, Marina, Lizzul, Alessandro Marco, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana" in Chemosphere, 260 (2020),
https://doi.org/10.1016/j.chemosphere.2020.127553 . .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Vojvodić, Snežana
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2408
AB  - Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron
translucent line inserted between two electron dense lines; the outermost layer is a mature mother
wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the
obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of
TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response
to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae
exposed to 5 Gy was not significantly different than in controls. A similar trend was
observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that
no effects of radiation on biomass, at doses applied here, could be observed.
PB  - Institute for Biological Research “Siniša Stanković” and others contributors
C3  - 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia
T1  - The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study
EP  - 153
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2408
ER  - 

@conference{
author = "Danilović Luković, Jelena and Zechmann, Bernd and Vojvodić, Snežana and Bogdanović Pristov, Jelena and Stanić, Marina and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron
translucent line inserted between two electron dense lines; the outermost layer is a mature mother
wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the
obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of
TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response
to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae
exposed to 5 Gy was not significantly different than in controls. A similar trend was
observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that
no effects of radiation on biomass, at doses applied here, could be observed.",
publisher = "Institute for Biological Research “Siniša Stanković” and others contributors",
journal = "14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia",
title = "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study",
pages = "153-152",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2408"
}

Danilović Luković, J., Zechmann, B., Vojvodić, S., Bogdanović Pristov, J., Stanić, M., Pittman, J. K.,& Spasojević, I.. (2019). The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia
Institute for Biological Research “Siniša Stanković” and others contributors., 152-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2408

Danilović Luković J, Zechmann B, Vojvodić S, Bogdanović Pristov J, Stanić M, Pittman JK, Spasojević I. The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia. 2019;:152-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2408 .

Danilović Luković, Jelena, Zechmann, Bernd, Vojvodić, Snežana, Bogdanović Pristov, Jelena, Stanić, Marina, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study" in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia (2019):152-153,
https://hdl.handle.net/21.15107/rcub_rimsi_2408 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2414
AB  - Microalgae are exposed to ionizing radiation from natural (e.g. radionuclides from the soil) and anthropogenic sources (such as radioactive waste, nuclear power accidents, etc). However, the mechanisms of adaptation to radiation are poorly understood. The aim of our study was to determine the effects of X-ray irradiation on the cell wall of Chlorella sorokiniana. C. sorokiniana (CCAP 211/8K) culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1 for 20 days, reaching stationary phase. Cultures were then exposed to X-rays at doses of 1, 2 and 5 Gy, left under the same conditions for additional 24 h, and analyzed using TEM (postfixation with 1% osmium tetroxide, embedded in resin for soft blocks, and stained with uranyl acetate and lead acetate), and gravimetry. The analysis of TEM micrographs showed that there were no significant changes in the thickness of trilaminar sheath for any of the used doses. However, the diameter of the fibrillar wall was increased in response to irradiation: 105.5 ± 7.5 nm for untreated samples vs. 135.0 ± 9.5 nm (p = 0.015) for microalgae exposed to 1 Gy, and 127.9 ± 7.4 nm (p = 0.036) for exposure to 2 Gy. The thickness of the cell wall in microalgae exposed to 5 Gy (118.6 ± 8.6 nm) was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed. It appears that Chlorella sorokiniana responds promptly to ionizing radiation by fortifying its ‘first line of defense’. The observed changes may be of particular interest for bioremediation, taking into account the capacity of the cell wall to bind water-soluble metals, including radionuclides.
PB  - Taylor & Francis Group
C3  - Journal of Experimental Botany
T1  - Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall
EP  - 166
IS  - Supp 1
SP  - 166
VL  - 54
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Bogdanović Pristov, Jelena and Stanić, Marina and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "Microalgae are exposed to ionizing radiation from natural (e.g. radionuclides from the soil) and anthropogenic sources (such as radioactive waste, nuclear power accidents, etc). However, the mechanisms of adaptation to radiation are poorly understood. The aim of our study was to determine the effects of X-ray irradiation on the cell wall of Chlorella sorokiniana. C. sorokiniana (CCAP 211/8K) culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1 for 20 days, reaching stationary phase. Cultures were then exposed to X-rays at doses of 1, 2 and 5 Gy, left under the same conditions for additional 24 h, and analyzed using TEM (postfixation with 1% osmium tetroxide, embedded in resin for soft blocks, and stained with uranyl acetate and lead acetate), and gravimetry. The analysis of TEM micrographs showed that there were no significant changes in the thickness of trilaminar sheath for any of the used doses. However, the diameter of the fibrillar wall was increased in response to irradiation: 105.5 ± 7.5 nm for untreated samples vs. 135.0 ± 9.5 nm (p = 0.015) for microalgae exposed to 1 Gy, and 127.9 ± 7.4 nm (p = 0.036) for exposure to 2 Gy. The thickness of the cell wall in microalgae exposed to 5 Gy (118.6 ± 8.6 nm) was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed. It appears that Chlorella sorokiniana responds promptly to ionizing radiation by fortifying its ‘first line of defense’. The observed changes may be of particular interest for bioremediation, taking into account the capacity of the cell wall to bind water-soluble metals, including radionuclides.",
publisher = "Taylor & Francis Group",
journal = "Journal of Experimental Botany",
title = "Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall",
pages = "166-166",
number = "Supp 1",
volume = "54",
doi = "10.1080/09670262.2019.1626628"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Bogdanović Pristov, J., Stanić, M., Pittman, J. K.,& Spasojević, I.. (2019). Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall. in Journal of Experimental Botany
Taylor & Francis Group., 54(Supp 1), 166-166.
https://doi.org/10.1080/09670262.2019.1626628

Vojvodić S, Danilović Luković J, Zechmann B, Bogdanović Pristov J, Stanić M, Pittman JK, Spasojević I. Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall. in Journal of Experimental Botany. 2019;54(Supp 1):166-166.
doi:10.1080/09670262.2019.1626628 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Bogdanović Pristov, Jelena, Stanić, Marina, Pittman, Jon K., Spasojević, Ivan, "Adaptive response of Chlorella sorokiniana to ionizing radiation on the level of cell wall" in Journal of Experimental Botany, 54, no. Supp 1 (2019):166-166,
https://doi.org/10.1080/09670262.2019.1626628 . .

TY  - JOUR
AU  - Janković, Tamara
AU  - Goč, Sanja
AU  - Mitic, Ninoslav
AU  - Danilović Luković, Jelena
AU  - Janković, Miroslava
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2454
AB  - Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs)
whose composition is, additionally, influenced by different physiological conditions. Aiming at the
molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction
factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference
could upgrade standardization of EVs isolated by different methods and intended for use in
biomedicine.
Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic
(sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
Prostasomes recovered in LAC-separated fractions were characterized according to the distribution
of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin
CD63, and total protein/glycoprotein composition.
Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing
in GGT activity and surface glycans were separated on the WGA column. Additional populations having
distinct profiles of total glycoproteins and which can be tracked down by ALP activity were
enriched on the Con A column. WGA-separated populations were similar in sPro-N and sPro-O,
whereas Con A-separated ones were strikingly different.
Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context
of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic
and oligozoospermic men.
PB  - Upsala Medical Society
T2  - Upsala Journal of Medical Sciences
T1  - Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men
EP  - 18
IS  - 1
SP  - 10
VL  - 125
DO  - 10.1080/03009734.2019.1690603
ER  - 

@article{
author = "Janković, Tamara and Goč, Sanja and Mitic, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava",
year = "2019",
abstract = "Background: Human seminal prostasomes are intrinsically heterogeneous extracellular vesicles (EVs)
whose composition is, additionally, influenced by different physiological conditions. Aiming at the
molecular properties of the prostasomal surface exemplified by glycan compositions as a possible distinction
factor, we applied lectin-affinity chromatography (LAC) as a new tool for their separation.
Since glycans, generally, exhibit various biological activities, introduction of glyco-parameters as reference
could upgrade standardization of EVs isolated by different methods and intended for use in
biomedicine.
Methods: Preparations of seminal prostasomes from normozoospermic (sPro-N) and oligozoospermic
(sPro-O) men were subjected to LAC on concanavalin A (Con A) and wheat germ agglutinin (WGA) columns.
Prostasomes recovered in LAC-separated fractions were characterized according to the distribution
of selected markers: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), tetraspanin
CD63, and total protein/glycoprotein composition.
Results: Two CD63-immunoreactive populations exhibiting prostasome signature bands but differing
in GGT activity and surface glycans were separated on the WGA column. Additional populations having
distinct profiles of total glycoproteins and which can be tracked down by ALP activity were
enriched on the Con A column. WGA-separated populations were similar in sPro-N and sPro-O,
whereas Con A-separated ones were strikingly different.
Conclusions: Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context
of Con A- and WGA-reactive glycans mark seminal prostasomes populations from normozoospermic
and oligozoospermic men.",
publisher = "Upsala Medical Society",
journal = "Upsala Journal of Medical Sciences",
title = "Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men",
pages = "18-10",
number = "1",
volume = "125",
doi = "10.1080/03009734.2019.1690603"
}

Janković, T., Goč, S., Mitic, N., Danilović Luković, J.,& Janković, M.. (2019). Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men. in Upsala Journal of Medical Sciences
Upsala Medical Society., 125(1), 10-18.
https://doi.org/10.1080/03009734.2019.1690603

Janković T, Goč S, Mitic N, Danilović Luković J, Janković M. Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men. in Upsala Journal of Medical Sciences. 2019;125(1):10-18.
doi:10.1080/03009734.2019.1690603 .

Janković, Tamara, Goč, Sanja, Mitic, Ninoslav, Danilović Luković, Jelena, Janković, Miroslava, "Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men" in Upsala Journal of Medical Sciences, 125, no. 1 (2019):10-18,
https://doi.org/10.1080/03009734.2019.1690603 . .

TY  - CONF
AU  - Jevtović (born Romanović), Mima
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Kovačević, Snežana
AU  - Stanić, Marina
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2812
AB  - Pozitivan biološki odgovor ćelije ili organizma na stres niskog intenziteta se naziva hormezis. U višim biljkama je hormezis kao odgovor na niske doze zračenja dobro dokumentovan ali ovakva ispitivanja do sada nisu rađena na mikroalgama. Mikroalge su grupa organizama koji se lako prilagođavaju nepovoljnim uslovima sredine i na taj način mogu proizvesti veliku biomasu pri niskom sadržaju nutrijenata.Cilj ove studije je bio da ispita odgovor jednoćelijske zelene alge Chlamydomonas reinhardtii (CCAP 11/32C) na niske doze X-zračenja. Ispitivan je efekat na produkciju biomase kao i na promene u zastupljenosti hlorofila a (Chl a) i b (Chl b) u različitim fazama rasta nakon izlaganja tretmanu. Alge su izlagane zračenju od 10; 20; 50 i 100 Gy pri brzinama od 0,565; 2,290 i 5,057 Gy/min. Tretman od 10 Gy je pri brzini od 0,565 Gy/min doveo do povećanja biomase za 21±15% 15 dana nakon tretmana, a pri brzini od 2,290 Gy/min do povećanja od 19±8% već nakon 5 dana. Tretman od 20 Gy je pri brzini od 0,565 Gy /min doveo do povećanja biomase od 22±12% nakon 15 dana. Svi ostali tretmani ili nisu imali efekta ili su doveli do smanjenja biomase. Meren je i efekat zračenja na sadržaj Chl a i Chl b (μg/mg suve mase). Sadržaj ovih pigmenata vremenom opada ali neki tretmani su doveli do smanjenja ovog efekta. Doza od 20 Gy pri brzini od 2,290 Gy/min je dala najbolje efekte na očuvanje Chl a i Chl b i oni su u odnosu na kontrolu bili veći 31±9% i 31±8%.
PB  - Srpsko biološko društvo
C3  - II Kongres biologa Srbije
T1  - Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii
EP  - 222
SP  - 222
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2812
ER  - 

@conference{
author = "Jevtović (born Romanović), Mima and Danilović Luković, Jelena and Morina, Arian and Kovačević, Snežana and Stanić, Marina and Spasojević, Ivan",
year = "2018",
abstract = "Pozitivan biološki odgovor ćelije ili organizma na stres niskog intenziteta se naziva hormezis. U višim biljkama je hormezis kao odgovor na niske doze zračenja dobro dokumentovan ali ovakva ispitivanja do sada nisu rađena na mikroalgama. Mikroalge su grupa organizama koji se lako prilagođavaju nepovoljnim uslovima sredine i na taj način mogu proizvesti veliku biomasu pri niskom sadržaju nutrijenata.Cilj ove studije je bio da ispita odgovor jednoćelijske zelene alge Chlamydomonas reinhardtii (CCAP 11/32C) na niske doze X-zračenja. Ispitivan je efekat na produkciju biomase kao i na promene u zastupljenosti hlorofila a (Chl a) i b (Chl b) u različitim fazama rasta nakon izlaganja tretmanu. Alge su izlagane zračenju od 10; 20; 50 i 100 Gy pri brzinama od 0,565; 2,290 i 5,057 Gy/min. Tretman od 10 Gy je pri brzini od 0,565 Gy/min doveo do povećanja biomase za 21±15% 15 dana nakon tretmana, a pri brzini od 2,290 Gy/min do povećanja od 19±8% već nakon 5 dana. Tretman od 20 Gy je pri brzini od 0,565 Gy /min doveo do povećanja biomase od 22±12% nakon 15 dana. Svi ostali tretmani ili nisu imali efekta ili su doveli do smanjenja biomase. Meren je i efekat zračenja na sadržaj Chl a i Chl b (μg/mg suve mase). Sadržaj ovih pigmenata vremenom opada ali neki tretmani su doveli do smanjenja ovog efekta. Doza od 20 Gy pri brzini od 2,290 Gy/min je dala najbolje efekte na očuvanje Chl a i Chl b i oni su u odnosu na kontrolu bili veći 31±9% i 31±8%.",
publisher = "Srpsko biološko društvo",
journal = "II Kongres biologa Srbije",
title = "Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii",
pages = "222-222",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2812"
}

Jevtović (born Romanović), M., Danilović Luković, J., Morina, A., Kovačević, S., Stanić, M.,& Spasojević, I.. (2018). Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii. in II Kongres biologa Srbije
Srpsko biološko društvo., 222-222.
https://hdl.handle.net/21.15107/rcub_rimsi_2812

Jevtović (born Romanović) M, Danilović Luković J, Morina A, Kovačević S, Stanić M, Spasojević I. Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii. in II Kongres biologa Srbije. 2018;:222-222.
https://hdl.handle.net/21.15107/rcub_rimsi_2812 .

Jevtović (born Romanović), Mima, Danilović Luković, Jelena, Morina, Arian, Kovačević, Snežana, Stanić, Marina, Spasojević, Ivan, "Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii" in II Kongres biologa Srbije (2018):222-222,
https://hdl.handle.net/21.15107/rcub_rimsi_2812 .

TY  - JOUR
AU  - Radovanovic, Anita
AU  - Kovačević Filipović, Milica
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Veličkoviić, Stefan
AU  - Andrejević, Jana
AU  - Danilović Luković, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2785
AB  - Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective
EP  - 89
IS  - 2
SP  - 80
VL  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2785
ER  - 

@article{
author = "Radovanovic, Anita and Kovačević Filipović, Milica and Milošević, Ivan and Lužajić, Tijana and Veličkoviić, Stefan and Andrejević, Jana and Danilović Luković, Jelena",
year = "2018",
abstract = "Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective",
pages = "89-80",
number = "2",
volume = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2785"
}

Radovanovic, A., Kovačević Filipović, M., Milošević, I., Lužajić, T., Veličkoviić, S., Andrejević, J.,& Danilović Luković, J.. (2018). Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785

Radovanovic A, Kovačević Filipović M, Milošević I, Lužajić T, Veličkoviić S, Andrejević J, Danilović Luković J. Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik. 2018;72(2):80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .

Radovanovic, Anita, Kovačević Filipović, Milica, Milošević, Ivan, Lužajić, Tijana, Veličkoviić, Stefan, Andrejević, Jana, Danilović Luković, Jelena, "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective" in Veterinarski Glasnik, 72, no. 2 (2018):80-89,
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Radovanovic, Anita
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
AU  - Korać, Aleksandra
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2698
AB  - The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell.
PB  - Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade
C3  - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
T1  - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
EP  - 57
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2698
ER  - 

@conference{
author = "Danilović Luković, Jelena and Radovanovic, Anita and Milošević, Ivan and Lužajić, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra",
year = "2018",
abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell.",
publisher = "Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade",
journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade",
title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2698"
}

Danilović Luković, J., Radovanovic, A., Milošević, I., Lužajić, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade., 57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_2698

Danilović Luković J, Radovanovic A, Milošević I, Lužajić T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_2698 .

Danilović Luković, Jelena, Radovanovic, Anita, Milošević, Ivan, Lužajić, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):57-57,
https://hdl.handle.net/21.15107/rcub_rimsi_2698 .

TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanovic, Anita
AU  - Danilović Luković, Jelena
AU  - Luzajic Bozinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Kovačević Filipović, Milica
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2471
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glycosaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
EP  - 320
IS  - 3
SP  - 301
VL  - 68
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanovic, Anita and Danilović Luković, Jelena and Luzajic Bozinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glycosaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
pages = "320-301",
number = "3",
volume = "68",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanovic, A., Danilović Luković, J., Luzajic Bozinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J.,& Kovačević Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanovic A, Danilović Luković J, Luzajic Bozinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Kovačević Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanovic, Anita, Danilović Luković, Jelena, Luzajic Bozinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Kovačević Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .

TY  - JOUR
AU  - Danilović Luković, Jelena
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2470
AB  - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUNEL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
EP  - 106
IS  - 1
SP  - 92
VL  - 67
DO  - 10.1515/acve-2017-0009
ER  - 

@article{
author = "Danilović Luković, Jelena",
year = "2017",
abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUNEL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats",
pages = "106-92",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0009"
}

Danilović Luković, J.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 67(1), 92-106.
https://doi.org/10.1515/acve-2017-0009

Danilović Luković J. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106.
doi:10.1515/acve-2017-0009 .

Danilović Luković, Jelena, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106,
https://doi.org/10.1515/acve-2017-0009 . .

TY  - CONF
AU  - Dekić, Milan
AU  - Radulović, Niko
AU  - Danilović Luković, Jelena
AU  - Stojanović, Dalibor
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2739
AB  - Volatile constituents obtained by autolysis of aerial and underground parts of D. sophia and the essential oil obtained by hydrodistillation of whole plant samples were analyzed in detail by GC and GC-MS. In total, 71 constituents were identified, accounting for more than 90% of the total peak areas in the chromatograms. Both aerial and underground autolysates contained considerable amounts of lignan arctigenin and cuticular wax compounds. The essential oil was dominated by glucosinolate breakdown product 4-pentenenitrile. Glucosinolate degradation products identified in the essential oil and autolysates, 3-butenyl isothiocyanate, 4-pentenenitrile and allyl isothiocyanate, suggested the presence of gluconapin and sinigrin in this species as the most likely “mustard oil” precursors.
PB  - University of Niš, Niš
C3  - Facta universitatis - series: Physics, Chemistry and Technology 15(2)
T1  - Volatile glucosinolate breakdown products and the essential oil of Descurainia sophia (L.) webb ex Prantl (Brassicaceae)
EP  - 102
IS  - 2
SP  - 95
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2739
ER  - 

@conference{
author = "Dekić, Milan and Radulović, Niko and Danilović Luković, Jelena and Stojanović, Dalibor",
year = "2017",
abstract = "Volatile constituents obtained by autolysis of aerial and underground parts of D. sophia and the essential oil obtained by hydrodistillation of whole plant samples were analyzed in detail by GC and GC-MS. In total, 71 constituents were identified, accounting for more than 90% of the total peak areas in the chromatograms. Both aerial and underground autolysates contained considerable amounts of lignan arctigenin and cuticular wax compounds. The essential oil was dominated by glucosinolate breakdown product 4-pentenenitrile. Glucosinolate degradation products identified in the essential oil and autolysates, 3-butenyl isothiocyanate, 4-pentenenitrile and allyl isothiocyanate, suggested the presence of gluconapin and sinigrin in this species as the most likely “mustard oil” precursors.",
publisher = "University of Niš, Niš",
journal = "Facta universitatis - series: Physics, Chemistry and Technology 15(2)",
title = "Volatile glucosinolate breakdown products and the essential oil of Descurainia sophia (L.) webb ex Prantl (Brassicaceae)",
pages = "102-95",
number = "2",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2739"
}

Dekić, M., Radulović, N., Danilović Luković, J.,& Stojanović, D.. (2017). Volatile glucosinolate breakdown products and the essential oil of Descurainia sophia (L.) webb ex Prantl (Brassicaceae). in Facta universitatis - series: Physics, Chemistry and Technology 15(2)
University of Niš, Niš., 15(2), 95-102.
https://hdl.handle.net/21.15107/rcub_rimsi_2739

Dekić M, Radulović N, Danilović Luković J, Stojanović D. Volatile glucosinolate breakdown products and the essential oil of Descurainia sophia (L.) webb ex Prantl (Brassicaceae). in Facta universitatis - series: Physics, Chemistry and Technology 15(2). 2017;15(2):95-102.
https://hdl.handle.net/21.15107/rcub_rimsi_2739 .

Dekić, Milan, Radulović, Niko, Danilović Luković, Jelena, Stojanović, Dalibor, "Volatile glucosinolate breakdown products and the essential oil of Descurainia sophia (L.) webb ex Prantl (Brassicaceae)" in Facta universitatis - series: Physics, Chemistry and Technology 15(2), 15, no. 2 (2017):95-102,
https://hdl.handle.net/21.15107/rcub_rimsi_2739 .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Milošević, Sandra
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2734
AB  - Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
T1  - Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population
EP  - 152
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2734
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Milošević, Sandra and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2017",
abstract = "Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb",
title = "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population",
pages = "152-152",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2734"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Milošević, S., Kovačević Filipović, M.,& Radovanovic, A.. (2017). Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734

Lužajić T, Milošević I, Danilović Luković J, Marković D, Milošević S, Kovačević Filipović M, Radovanovic A. Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Milošević, Sandra, Kovačević Filipović, Milica, Radovanovic, Anita, "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):152-152,
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .