TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Janković, Tamara
AU  - Goč, Sanja
AU  - Janjić, Filip
AU  - Mitic, Ninoslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2732
AB  - Glycans, complex carbohydrates bound to lipids and proteins, play important roles in biological processes.
Prostasomes, extracellular vesicles from prostate epithelial cells, have a glycan composition influenced by
their cellular origin and by the composition of the seminal plasma into which they are secreted. We
hypothesized that the membrane-associated glycans could be used as selective targets for separation of
prostasomes and as relevant parameter for distinction of their populations.
Prostasomes from seminal plasma of normozoospermic and oligozoospermic men were separated by
ion-exchange- and lectin-affinity chromatography using concanavalin A (ConA) lectin, specific for
mannosylated structures, and wheat germ agglutinin (WGA), specific for sialic acid. The presence of
tetraspanins (CD63, CD9 and CD81), galectin-3 (gal-3) and gamma-glutamyl transferase (GGT) as known
vesicle markers were monitored in association with distinct glycans. Their distribution was also analysed upon
treatment of prostasomes with non-ionic detergent.
Membrane-associated GGT in the context of Con A- and WGA-reactive glycans mark prostasome
populations from normozoospermic and oligozoospermic men. The assembly of tetraspanins, gal-3, and
distinct N-glycans defines the solubilisation signature of prostasomes. WGA-reactive glycoproteins
co-localize with CD9 and gal-3 in detergent-resistant domains, whereas ConA-reactive glycoproteins were
distributed in detergent-sensitive domains along with CD63 and GGT. Subtle differences in the
composition/presentation of examined molecules made difference among vesicles sharing the same physical properties in each group as well as between them.
The results obtained suggest the potential of glyco-parameters as reference markers for EVs populations.
PB  - Medical University Graz, Austria
C3  - Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria
T1  - Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes
EP  - 52
SP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2732
ER  - 

@conference{
author = "Danilović Luković, Jelena and Janković, Tamara and Goč, Sanja and Janjić, Filip and Mitic, Ninoslav",
year = "2023",
abstract = "Glycans, complex carbohydrates bound to lipids and proteins, play important roles in biological processes.
Prostasomes, extracellular vesicles from prostate epithelial cells, have a glycan composition influenced by
their cellular origin and by the composition of the seminal plasma into which they are secreted. We
hypothesized that the membrane-associated glycans could be used as selective targets for separation of
prostasomes and as relevant parameter for distinction of their populations.
Prostasomes from seminal plasma of normozoospermic and oligozoospermic men were separated by
ion-exchange- and lectin-affinity chromatography using concanavalin A (ConA) lectin, specific for
mannosylated structures, and wheat germ agglutinin (WGA), specific for sialic acid. The presence of
tetraspanins (CD63, CD9 and CD81), galectin-3 (gal-3) and gamma-glutamyl transferase (GGT) as known
vesicle markers were monitored in association with distinct glycans. Their distribution was also analysed upon
treatment of prostasomes with non-ionic detergent.
Membrane-associated GGT in the context of Con A- and WGA-reactive glycans mark prostasome
populations from normozoospermic and oligozoospermic men. The assembly of tetraspanins, gal-3, and
distinct N-glycans defines the solubilisation signature of prostasomes. WGA-reactive glycoproteins
co-localize with CD9 and gal-3 in detergent-resistant domains, whereas ConA-reactive glycoproteins were
distributed in detergent-sensitive domains along with CD63 and GGT. Subtle differences in the
composition/presentation of examined molecules made difference among vesicles sharing the same physical properties in each group as well as between them.
The results obtained suggest the potential of glyco-parameters as reference markers for EVs populations.",
publisher = "Medical University Graz, Austria",
journal = "Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria",
title = "Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes",
pages = "52-52",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2732"
}

Danilović Luković, J., Janković, T., Goč, S., Janjić, F.,& Mitic, N.. (2023). Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes. in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria
Medical University Graz, Austria., 52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2732

Danilović Luković J, Janković T, Goč S, Janjić F, Mitic N. Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes. in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria. 2023;:52-52.
https://hdl.handle.net/21.15107/rcub_rimsi_2732 .

Danilović Luković, Jelena, Janković, Tamara, Goč, Sanja, Janjić, Filip, Mitic, Ninoslav, "Surface-Associated Glycans as a Possible Distinct Factor for Establishing the Molecular Properties of Prostasomes" in Joint Meeting of ASEV, HSEV, SiNEV and SrbEV, Small New World 2.0, Graz, Austria (2023):52-52,
https://hdl.handle.net/21.15107/rcub_rimsi_2732 .