Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective
Аутори
Radovanovic, AnitaKovačević Filipović, Milica
Milošević, Ivan
Lužajić, Tijana
Veličkoviić, Stefan
Andrejević, Jana
Danilović Luković, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Resul...ts and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.
Кључне речи:
Apoptosis / maternal hypothyroidism / proliferationИзвор:
Veterinarski Glasnik, 2018, 72, 2, 80-89Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Radovanovic, Anita AU - Kovačević Filipović, Milica AU - Milošević, Ivan AU - Lužajić, Tijana AU - Veličkoviić, Stefan AU - Andrejević, Jana AU - Danilović Luković, Jelena PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2785 AB - Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodeling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective EP - 89 IS - 2 SP - 80 VL - 72 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2785 ER -
@article{ author = "Radovanovic, Anita and Kovačević Filipović, Milica and Milošević, Ivan and Lužajić, Tijana and Veličkoviić, Stefan and Andrejević, Jana and Danilović Luković, Jelena", year = "2018", abstract = "Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration and remodeling after each ovulation during the whole reproductive period. This process increases the risk of one of the most common ovarian tumors in women and the female dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation and cell death during folliculogenesis in the early neonatal period, we aimed to analyze its effect on OSE morphology and dynamics. Materials and Methods. The study was performed on newborn (24-h-old) and neonatal (4-day-old) female rats, a randomized trial between the control and hypothyroid groups, born under controlled circumstances and hypothyroid mothers, respectively. Their ovaries were analyzed histologically and processed to determine the OSE cell height as an average value of four measurement points. Also, the immunopositivity of the proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively. Results and Conclusions. No major structural differences of OSE were found between groups within the given ages except for a slight increment of OSE cell height and incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid animals of both ages in comparison to the controls. Moreover, only scarce OSE cells were caspase-3 positive in both groups and ages, with no difference in immunopositivity. Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent cell removal, which may serve as a premise for future investigation of potential carcinogenesis, in terms of prevention and treatment of ovarian cancer.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective", pages = "89-80", number = "2", volume = "72", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2785" }
Radovanovic, A., Kovačević Filipović, M., Milošević, I., Lužajić, T., Veličkoviić, S., Andrejević, J.,& Danilović Luković, J.. (2018). Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 80-89. https://hdl.handle.net/21.15107/rcub_rimsi_2785
Radovanovic A, Kovačević Filipović M, Milošević I, Lužajić T, Veličkoviić S, Andrejević J, Danilović Luković J. Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik. 2018;72(2):80-89. https://hdl.handle.net/21.15107/rcub_rimsi_2785 .
Radovanovic, Anita, Kovačević Filipović, Milica, Milošević, Ivan, Lužajić, Tijana, Veličkoviić, Stefan, Andrejević, Jana, Danilović Luković, Jelena, "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective" in Veterinarski Glasnik, 72, no. 2 (2018):80-89, https://hdl.handle.net/21.15107/rcub_rimsi_2785 .