Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity
Аутори
Janković, TamaraDanilović Luković, Jelena
Goč, Sanja
Mitić, Ninoslav
Hajduković, Ljiljana
Janković, Miroslava
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background. Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration
and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely
explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained
on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of
selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between
known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should
contribute to define them together as possible multimarkers.
Methods. GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed
using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A).
Results. Widely distributed GGT... (with two to three partially resolved peaks), which may correspond to high molecular
mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and
small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive
glycans, as well as mucin-associated antigens CA19-9 and CA125.
Conclusions. GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle
fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated
glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms
of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT.
Кључне речи:
human seminal plasma / gamma-glutamyltransferase / sialylated glycans / CA19-9 / CA125Извор:
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 2023Издавач:
- Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic
Финансирање / пројекти:
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Janković, Tamara AU - Danilović Luković, Jelena AU - Goč, Sanja AU - Mitić, Ninoslav AU - Hajduković, Ljiljana AU - Janković, Miroslava PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2497 AB - Background. Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should contribute to define them together as possible multimarkers. Methods. GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A). Results. Widely distributed GGT (with two to three partially resolved peaks), which may correspond to high molecular mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive glycans, as well as mucin-associated antigens CA19-9 and CA125. Conclusions. GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT. PB - Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic T2 - Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia T1 - Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity DO - 10.5507/bp.2023.031 ER -
@article{ author = "Janković, Tamara and Danilović Luković, Jelena and Goč, Sanja and Mitić, Ninoslav and Hajduković, Ljiljana and Janković, Miroslava", year = "2023", abstract = "Background. Gamma-glutamyltransferase (GGT) is a well-known laboratory biomarker. In spite of high concentration and the possible biomedical importance of estimating GGT in human seminal plasma (hSP), it has not been widely explored in reproductive physiology. This study aimed to complement existing data on its diversity, previously obtained on seminal extracellular vesicles, by analyzing matched soluble fraction of hSP. The GGT-associated patterns of selected glycoproteins were analyzed in order to establish an adjunct referent parameter for differentiation between known high molecular mass forms of GGT. Getting insight into distinct GGT-associated glycoprotein patterns should contribute to define them together as possible multimarkers. Methods. GGT forms in soluble, membrane-free-fraction isolated form hSP of normozoospermic men were analyzed using gel filtration and lectin blotting using WGA (wheat germ agglutinin) and Con A (concanavalin A). Results. Widely distributed GGT (with two to three partially resolved peaks), which may correspond to high molecular mass aggregates, were detected. GGT-associated patterns of selected glycoproteins (at position of big, medium, and small-GGT) all comprised high molecular mass WGA-reactive smears, but differed in the presence of Con A-reactive glycans, as well as mucin-associated antigens CA19-9 and CA125. Conclusions. GGT contributes to several molecular patterns that differ between the soluble and extracellular vesicle fractions of hSP. Their glycobiochemical heterogeneity is due to difference in the presence of distinct sialylated and mannosylated glycans. Moreover, GGT-associated glycoprotein patterns differentiate between high molecular mass forms of GGT in the soluble fraction of hSP. They hold promise as possible targets for increasing biomarker potential of GGT.", publisher = "Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic", journal = "Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia", title = "Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity", doi = "10.5507/bp.2023.031" }
Janković, T., Danilović Luković, J., Goč, S., Mitić, N., Hajduković, L.,& Janković, M.. (2023). Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity. in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia Faculty of Medicine and Dentistry Palacký University Olomouc, Czech Republic.. https://doi.org/10.5507/bp.2023.031
Janković T, Danilović Luković J, Goč S, Mitić N, Hajduković L, Janković M. Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity. in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia. 2023;. doi:10.5507/bp.2023.031 .
Janković, Tamara, Danilović Luković, Jelena, Goč, Sanja, Mitić, Ninoslav, Hajduković, Ljiljana, Janković, Miroslava, "Gamma-glutamyltransferase-associated glycoprotein patterns in human seminal plasma of normozoospermic men: a new aspect of biomarker heterogeneity" in Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia (2023), https://doi.org/10.5507/bp.2023.031 . .