TY  - JOUR
AU  - Radovanovic, Anita
AU  - Kovačević Filipović, Milica
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Veličkoviić, Stefan
AU  - Andrejević, Jana
AU  - Danilović Luković, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2785
AB  - Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective
EP  - 89
IS  - 2
SP  - 80
VL  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2785
ER  - 

@article{
author = "Radovanovic, Anita and Kovačević Filipović, Milica and Milošević, Ivan and Lužajić, Tijana and Veličkoviić, Stefan and Andrejević, Jana and Danilović Luković, Jelena",
year = "2018",
abstract = "Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective",
pages = "89-80",
number = "2",
volume = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2785"
}

Radovanovic, A., Kovačević Filipović, M., Milošević, I., Lužajić, T., Veličkoviić, S., Andrejević, J.,& Danilović Luković, J.. (2018). Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785

Radovanovic A, Kovačević Filipović M, Milošević I, Lužajić T, Veličkoviić S, Andrejević J, Danilović Luković J. Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik. 2018;72(2):80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .

Radovanovic, Anita, Kovačević Filipović, Milica, Milošević, Ivan, Lužajić, Tijana, Veličkoviić, Stefan, Andrejević, Jana, Danilović Luković, Jelena, "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective" in Veterinarski Glasnik, 72, no. 2 (2018):80-89,
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .

TY  - JOUR
AU  - Danilović Luković, Jelena
AU  - Korać, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Puškaš, Nela
AU  - Kovačević Filipović, Milica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2446
AB  - tThyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previ-ously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian folliclesin female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentra-tion of TH on primordial follicles pool formation during neonatal and early infantile period of rat pupswas not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negativeinfluence on primordial follicles pool formation and population of resting oocytes in female rat offspring.The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female ratpups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively.Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM).Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicleswere determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin redstaining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum(sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyltransferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclearantigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of fol-licles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes andnests, an increased number of primordial follicles and a decreased number of primary and secondary fol-licles, while in early infantile period, increased number of primary, secondary and preantral follicles werefound. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content ofdilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups wasincreased. Apoptosis markers have not shown significant difference between groups but PCNA had anincreased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Lightand electron microscopy analysis indicate that previously detected premature ovarian follicular atresia inpre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial folliclesfollowed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Micron
T1  - Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach
EP  - 42
SP  - 33
VL  - 90
DO  - 10.1016/j.micron.2016.08.007
ER  - 

@article{
author = "Danilović Luković, Jelena and Korać, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Puškaš, Nela and Kovačević Filipović, Milica",
year = "2016",
abstract = "tThyroid hormones (TH) are one of the key factors for normal prenatal development in mammals. Previ-ously, we showed that subclinical maternal hypothyroidism leads to premature atresia of ovarian folliclesin female rat offspring in the pre-pubertal and pubertal periods. The influence of decreased concentra-tion of TH on primordial follicles pool formation during neonatal and early infantile period of rat pupswas not investigated previously. Maternal hypothyroidism during pregnancy has irreversible negativeinfluence on primordial follicles pool formation and population of resting oocytes in female rat offspring.The study was done on neonatal and early infantile control (n-10) and hypothyroid (n-10) female ratpups derived from control (n-6) and propylthiouracil (PTU) treated pregnant dams (n-6), respectively.Ovaries of all pups were removed and processed for light and transmission electron microscopy (TEM).Number of nests, oogonia and oocytes per nest, primordial, primary, secondary and preantral follicleswere determined. Screening for overall calcium presence in ovarian tissue was done using Alizarin redstaining. Morphology and volume density of nucleus, mitochondria and smooth endoplasmic reticulum(sER) in the oocytes in primordial follicles was also assessed. Caspase-3 and terminal deoxynucleotidyltransferase dUTP nick end labelling (TUNEL), both markers for apoptosis, and proliferating cell nuclearantigen (PCNA) for proliferation were determined in oocytes and granulosa cells in different type of fol-licles. In neonatal period, ovaries of hypothyroid pups had a decreased number of oogonia, oocytes andnests, an increased number of primordial follicles and a decreased number of primary and secondary fol-licles, while in early infantile period, increased number of primary, secondary and preantral follicles werefound. Alizarin red staining was intense in hypothyroid neonatal rats that also had the highest content ofdilated sER. Number of mitochondria with altered morphology in both groups of hypothyroid pups wasincreased. Apoptosis markers have not shown significant difference between groups but PCNA had anincreased expression in the oocytes and granulosa cells in primordial follicles of hypothyroid rats. Lightand electron microscopy analysis indicate that previously detected premature ovarian follicular atresia inpre-pubertal and pubertal hypothyroid rats is preceded with premature formation of primordial folliclesfollowed by slight changes on sER and mitochondria in examined oocytes, and increased expression of PCNA.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Micron",
title = "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach",
pages = "42-33",
volume = "90",
doi = "10.1016/j.micron.2016.08.007"
}

Danilović Luković, J., Korać, A., Milošević, I., Lužajić, T., Puškaš, N.,& Kovačević Filipović, M.. (2016). Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron
Pergamon-Elsevier Science Ltd, Oxford., 90, 33-42.
https://doi.org/10.1016/j.micron.2016.08.007

Danilović Luković J, Korać A, Milošević I, Lužajić T, Puškaš N, Kovačević Filipović M. Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach. in Micron. 2016;90:33-42.
doi:10.1016/j.micron.2016.08.007 .

Danilović Luković, Jelena, Korać, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Puškaš, Nela, Kovačević Filipović, Milica, "Altered state of primordial follicles in neonatal and early infantile rats due to maternal hypothyroidism: Light and electron microscopy approach" in Micron, 90 (2016):33-42,
https://doi.org/10.1016/j.micron.2016.08.007 . .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2796
AB  - Uvod: Poznato je da su tireoidni hormoni (TH) od esencijalnog značaja za razvoj, funkciju i diferencijaciju svih ćelija u organizmu u toku prenatalnog i postnatalnog života. Prethodna istraživanja dovode u vezu smanjen nivo TH i razvoj reumatoidnog artritisa i drugih oboljenja zglobova, međutim mehanizam nastanka navedenih promena još uvek nije razjašnjen u potpunosti. U sinovijalnim membranama (SM) zglobova zahvaćenih reumatoidnim artritisom, utvrđeno je povećanje broja ćelija koje eksprimiraju PCNA (proliferating cell nuclear antigen). Cilj ovog istraživanja je bio da se definiše uticaj subkliničkog hipotireoidizma majki tokom graviditeta na SM novorođenih jedinki, s obzirom da u dosadašnjim istraživanjima ovaj efekat nije dovoljno proučen.
Materijal i metode: Subklinički hipotireoidizam izazvan je kod gravidnih jedinki Albino Oxford pacova, dodavanjem propiltiouracila kroz vodu za piće (1,5 mg/l) počevši od prvog dana graviditeta i tokom laktacije. Kontrolna grupa pacova nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Sva histološka ispitivanja sprovedena su na parafinskim presecima debljine 5µm obojenim standardnim hematoksilin/eozin histohemijskim bojenjem, čija je analiza sprovedena na svetlosnom mikroskopu (Olympus BX53). Ekspresija PCNA je pokazana inunohistohemijski (Santa Cruz Biotechnology). Kvantifikacija ekspresije PCNA izvršena je pomoću softvera Image J.
Rezultati: Pregledom preseka bojenih hematoksilin/eozinom nisu uočene značajne razlike u građi SM između kontrolne i tretirane grupe. Broj ćelija koje eksprimiraju PCNA u SM tretiranih životinja je značajno veća od broja ćelija u SM kontrolnih životinja. 
Zaključak: Povećana ekspresija PCNA ukazuje na intenziviranu proliferaciju ćelija SM novorođenih pacova starosti 7 dana. Ovaj rezultat može biti osnova za nastavak istraživanja uticaja hipotireoidizma na razviće SM.
PB  - Medicinski fakultet Novi Sad
C3  - 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
T1  - Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2796
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2016",
abstract = "Uvod: Poznato je da su tireoidni hormoni (TH) od esencijalnog značaja za razvoj, funkciju i diferencijaciju svih ćelija u organizmu u toku prenatalnog i postnatalnog života. Prethodna istraživanja dovode u vezu smanjen nivo TH i razvoj reumatoidnog artritisa i drugih oboljenja zglobova, međutim mehanizam nastanka navedenih promena još uvek nije razjašnjen u potpunosti. U sinovijalnim membranama (SM) zglobova zahvaćenih reumatoidnim artritisom, utvrđeno je povećanje broja ćelija koje eksprimiraju PCNA (proliferating cell nuclear antigen). Cilj ovog istraživanja je bio da se definiše uticaj subkliničkog hipotireoidizma majki tokom graviditeta na SM novorođenih jedinki, s obzirom da u dosadašnjim istraživanjima ovaj efekat nije dovoljno proučen.
Materijal i metode: Subklinički hipotireoidizam izazvan je kod gravidnih jedinki Albino Oxford pacova, dodavanjem propiltiouracila kroz vodu za piće (1,5 mg/l) počevši od prvog dana graviditeta i tokom laktacije. Kontrolna grupa pacova nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Sva histološka ispitivanja sprovedena su na parafinskim presecima debljine 5µm obojenim standardnim hematoksilin/eozin histohemijskim bojenjem, čija je analiza sprovedena na svetlosnom mikroskopu (Olympus BX53). Ekspresija PCNA je pokazana inunohistohemijski (Santa Cruz Biotechnology). Kvantifikacija ekspresije PCNA izvršena je pomoću softvera Image J.
Rezultati: Pregledom preseka bojenih hematoksilin/eozinom nisu uočene značajne razlike u građi SM između kontrolne i tretirane grupe. Broj ćelija koje eksprimiraju PCNA u SM tretiranih životinja je značajno veća od broja ćelija u SM kontrolnih životinja. 
Zaključak: Povećana ekspresija PCNA ukazuje na intenziviranu proliferaciju ćelija SM novorođenih pacova starosti 7 dana. Ovaj rezultat može biti osnova za nastavak istraživanja uticaja hipotireoidizma na razviće SM.",
publisher = "Medicinski fakultet Novi Sad",
journal = "5th Congress of Serbian Anatomical Society with international participation, Novi Sad",
title = "Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2796"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Radovanovic, A.. (2016). Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
Medicinski fakultet Novi Sad., 96-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2796

Lužajić T, Milošević I, Danilović Luković J, Marković D, Kovačević Filipović M, Radovanovic A. Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad. 2016;:96-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2796 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Radovanovic, Anita, "Uticaj subkliničkog hipotireoidizma majki na razvoj sinovijalne membrane novorođenih pacova starih 7 dana" in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad (2016):96-96,
https://hdl.handle.net/21.15107/rcub_rimsi_2796 .

TY  - CONF
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3037
AB  - Uvod: Tireoidni hormoni (TH) imaju važnu ulogu u postnatalnom razvoju i očuvanju funkcije skeletno zglobnog sistema. U procesu endohondralnog okostavanja, TH svojim delovanjem na hondrocite podstiču rast kostiju. Brojna istraživanja su pokazala efekte hipotireoidizma na ovaj proces na različitim eksperimentalnim modelima, a cilj ovog istraživanja je bio da definiše uticaj subkliničkog hipotireoidizma majki u toku graviditeta i laktacije na proces endohondralnog okoštavanja tibije mladunaca starih sedam dana. 
Materijal i metode: Subklinički hipotireoidizam izazvan je kod gravidnih jedinki Albino Oxford pacova, dodavanjem propiltiouracila u vodu za piće (1,5 mg/l), od prvog dana graviditeta i tokom laktacije, dok kontrolna grupa nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Histološka ispitivanja sprovedena su na 5µm debelim parafinskim presecima tibije bojenim hematoksilin/eozinom. Visina proksimalne epifizne ploče merena je na presecima na svetlosnom mikroskopu (Olympus BX53), uz odgovarajući softver SellSensEntry (Olympus). Ekspresija kolagena X (ColX) je pokazana in situ hibridizacijom. Kvantifikacija ekspresije ColX izvršena je pomoću softvera Image J.
Rezultati: Visina epifizne ploče je značajno niža kod mladunaca iz tretirane grupe u poređenju sa kontrolnom grupom. Merenjem površine koju zahvata pozitivan signal u odnosu na ukupnu površinu zone hipertrofičnih hondrocita utvrđeno je značajno smanjenje ekspresije ColX kod jedinki iz tretirane grupe. 
Zaključak: Ovi rezultati ukazuju da subklinički hipotireoidizam majki za vreme graviditeta remeti hipertrofičnu diferencijaciju hondrocita i fiziološki razvoj epifizne ploče.
PB  - Medicinski fakultet Novi Sad
C3  - 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
T1  - Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3037
ER  - 

@conference{
author = "Milošević, Ivan and Lužajić, Tijana and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2016",
abstract = "Uvod: Tireoidni hormoni (TH) imaju važnu ulogu u postnatalnom razvoju i očuvanju funkcije skeletno zglobnog sistema. U procesu endohondralnog okostavanja, TH svojim delovanjem na hondrocite podstiču rast kostiju. Brojna istraživanja su pokazala efekte hipotireoidizma na ovaj proces na različitim eksperimentalnim modelima, a cilj ovog istraživanja je bio da definiše uticaj subkliničkog hipotireoidizma majki u toku graviditeta i laktacije na proces endohondralnog okoštavanja tibije mladunaca starih sedam dana. 
Materijal i metode: Subklinički hipotireoidizam izazvan je kod gravidnih jedinki Albino Oxford pacova, dodavanjem propiltiouracila u vodu za piće (1,5 mg/l), od prvog dana graviditeta i tokom laktacije, dok kontrolna grupa nije tretirana. Mladunci su žrtvovani sedmog dana nakon rođenja. Histološka ispitivanja sprovedena su na 5µm debelim parafinskim presecima tibije bojenim hematoksilin/eozinom. Visina proksimalne epifizne ploče merena je na presecima na svetlosnom mikroskopu (Olympus BX53), uz odgovarajući softver SellSensEntry (Olympus). Ekspresija kolagena X (ColX) je pokazana in situ hibridizacijom. Kvantifikacija ekspresije ColX izvršena je pomoću softvera Image J.
Rezultati: Visina epifizne ploče je značajno niža kod mladunaca iz tretirane grupe u poređenju sa kontrolnom grupom. Merenjem površine koju zahvata pozitivan signal u odnosu na ukupnu površinu zone hipertrofičnih hondrocita utvrđeno je značajno smanjenje ekspresije ColX kod jedinki iz tretirane grupe. 
Zaključak: Ovi rezultati ukazuju da subklinički hipotireoidizam majki za vreme graviditeta remeti hipertrofičnu diferencijaciju hondrocita i fiziološki razvoj epifizne ploče.",
publisher = "Medicinski fakultet Novi Sad",
journal = "5th Congress of Serbian Anatomical Society with international participation, Novi Sad",
title = "Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3037"
}

Milošević, I., Lužajić, T., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Radovanovic, A.. (2016). Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad
Medicinski fakultet Novi Sad., 96-96.
https://hdl.handle.net/21.15107/rcub_rimsi_3037

Milošević I, Lužajić T, Danilović Luković J, Marković D, Kovačević Filipović M, Radovanovic A. Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana. in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad. 2016;:96-96.
https://hdl.handle.net/21.15107/rcub_rimsi_3037 .

Milošević, Ivan, Lužajić, Tijana, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Radovanovic, Anita, "Uticaj subkliničkog hipotireoidizma majki na hipertrofičnu diferencijaciju hondrocita u proksimalnoj epifiznoj ploči tibije novorođenih pacova starih 7 dana" in 5th Congress of Serbian Anatomical Society with international participation, Novi Sad (2016):96-96,
https://hdl.handle.net/21.15107/rcub_rimsi_3037 .

TY  - JOUR
AU  - Lužajić, Tijana
AU  - Danilović Luković, Jelena
AU  - Katić, Marko
AU  - Božić, Tatjana
AU  - Kovačević Filipović, Milica
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2749
AB  - Cilj ovog istraživanja je bio da na osnovu promena u apsolutnom
broju pojedinih vrsta leukocita u peripartalnom periodu kod krava sa
i bez retencije placente utvrdi da li je zaostajanje posteljice povezano
sa subkliničkom inflamatornom reakcijom u prepartalnom periodu.
Šesnaest visokosteonih krava holštajn rase, starosti od 3 do 9 godina,
koje nisu pokazivale kliničke znake oboljenja pre teljenja, posle
porođaja je podeljeno u dve grupe: prvu grupu (n=9) činile su životinje
koje nisu imale retenciju placente, niti bilo kakvu klinički vidljivu
inflamaciju posle porođaja; u drugoj grupi (n=7) bile su krave sa retencijom
placente. Krv je uzorkovana 3 puta pre teljenja u razmaku
od nedelju
dana i jednom 24 sata po porođaju. Ukupan broj leukocita
i apsolutan broj segmentiranih i nesegmentiranih (mladih) neutrofilnih
granulocita
(NG), kao i broj limfocita i monocita određen je standarnim
laboratorijskim tehnikama. Rezultati istraživanja su pokazali
da je grupa krava kod koje je posle porođaja nastala retencija placente,
u trećoj, drugoj i u poslednjoj nedelji pred porođaj imala blago
povišen broj segmentiranih NG, a podjednak broj mladih NG u odnosu
na grupu kod koje se nije javila retencija. Kod obe grupe krava, 24
sata nakon porođaja, došlo je do značajnog pada ukupnog broja leukocita
i broja segmentiranih NG, a porasta broja mladih NG. Na osnovu
navedenih rezultata može se smatrati da životinje kod kojih je
u prvih 24 sata dijagnostikovana retencija placente, pre porođaja nisu
imale sistemsku subkliničku inflamatornu reakciju. Dvadeset i četiri
sata posle porođaja sve životinje su imale subkliničku sistemsku
inflamatornu reakciju. Takođe, kod životinja sa retencijom placente,u prvih 24 sata posle porođaja, inflamatorna reakcija, evaluirana kroz
ispitivane parametre, nije bila jačeg intenziteta u odnosu na krave kod
kojih ovo patološko stanje nije postojalo.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Peripartal leukogram in cows with and without retained placenta
EP  - 54
IS  - 1-2
SP  - 43
VL  - 68
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2749
ER  - 

@article{
author = "Lužajić, Tijana and Danilović Luković, Jelena and Katić, Marko and Božić, Tatjana and Kovačević Filipović, Milica",
year = "2014",
abstract = "Cilj ovog istraživanja je bio da na osnovu promena u apsolutnom
broju pojedinih vrsta leukocita u peripartalnom periodu kod krava sa
i bez retencije placente utvrdi da li je zaostajanje posteljice povezano
sa subkliničkom inflamatornom reakcijom u prepartalnom periodu.
Šesnaest visokosteonih krava holštajn rase, starosti od 3 do 9 godina,
koje nisu pokazivale kliničke znake oboljenja pre teljenja, posle
porođaja je podeljeno u dve grupe: prvu grupu (n=9) činile su životinje
koje nisu imale retenciju placente, niti bilo kakvu klinički vidljivu
inflamaciju posle porođaja; u drugoj grupi (n=7) bile su krave sa retencijom
placente. Krv je uzorkovana 3 puta pre teljenja u razmaku
od nedelju
dana i jednom 24 sata po porođaju. Ukupan broj leukocita
i apsolutan broj segmentiranih i nesegmentiranih (mladih) neutrofilnih
granulocita
(NG), kao i broj limfocita i monocita određen je standarnim
laboratorijskim tehnikama. Rezultati istraživanja su pokazali
da je grupa krava kod koje je posle porođaja nastala retencija placente,
u trećoj, drugoj i u poslednjoj nedelji pred porođaj imala blago
povišen broj segmentiranih NG, a podjednak broj mladih NG u odnosu
na grupu kod koje se nije javila retencija. Kod obe grupe krava, 24
sata nakon porođaja, došlo je do značajnog pada ukupnog broja leukocita
i broja segmentiranih NG, a porasta broja mladih NG. Na osnovu
navedenih rezultata može se smatrati da životinje kod kojih je
u prvih 24 sata dijagnostikovana retencija placente, pre porođaja nisu
imale sistemsku subkliničku inflamatornu reakciju. Dvadeset i četiri
sata posle porođaja sve životinje su imale subkliničku sistemsku
inflamatornu reakciju. Takođe, kod životinja sa retencijom placente,u prvih 24 sata posle porođaja, inflamatorna reakcija, evaluirana kroz
ispitivane parametre, nije bila jačeg intenziteta u odnosu na krave kod
kojih ovo patološko stanje nije postojalo.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Peripartal leukogram in cows with and without retained placenta",
pages = "54-43",
number = "1-2",
volume = "68",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2749"
}

Lužajić, T., Danilović Luković, J., Katić, M., Božić, T.,& Kovačević Filipović, M.. (2014). Peripartal leukogram in cows with and without retained placenta. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(1-2), 43-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2749

Lužajić T, Danilović Luković J, Katić M, Božić T, Kovačević Filipović M. Peripartal leukogram in cows with and without retained placenta. in Veterinarski Glasnik. 2014;68(1-2):43-54.
https://hdl.handle.net/21.15107/rcub_rimsi_2749 .

Lužajić, Tijana, Danilović Luković, Jelena, Katić, Marko, Božić, Tatjana, Kovačević Filipović, Milica, "Peripartal leukogram in cows with and without retained placenta" in Veterinarski Glasnik, 68, no. 1-2 (2014):43-54,
https://hdl.handle.net/21.15107/rcub_rimsi_2749 .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Todorović, Vera
AU  - Drndarević, Neda
AU  - Roksandić, Dragutin
AU  - Radovanovic, Anita
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2737
AB  - Background: The aim of our study was to examine the effect of mild maternal hypothyroidism on the apoptosis of the oocytes in the ovaries of rats in the early postnatal period during formation of oocytes and follicles.
Materials and Methods: Hypothyroidism was induced in pregnant and lactating rats by feeding 1.5mg/L propylthiouracil (PTU) through drinking water. Ovaries were obtained from newborns on postnatal day (PND) 0, 4 and 7. The degree of oocyte apoptosis was measured from the largest cross sections (n=2) from each ovary using by Colorimetric - TdT (terminal deoxynucleoid transferase) Enzyme in situ cell death detection kit (Merck).
Results: This preliminary result suggests that hypothyroidism induce increase of the total oocyte number from 4. day in the experimental group while the number of apoptotic oocytes is the lowest that day. However, the highest percentage of apoptosis is noticed on day 0. in control group.
Conclusion: In our further investigation we will notice whether the morphological changes induced on the oocytes appear before or after development of primordial follicles as a result of the impacts of maternal hypothyroidism on folliculogenesis of their offspring.
PB  - Medical University of Graz, Austria
C3  - 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist
T1  - Apoptosis of the oocytes of hypothyroid neonatal rats
EP  - 36
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2737
ER  - 

@conference{
author = "Danilović Luković, Jelena and Marković, Danica and Todorović, Vera and Drndarević, Neda and Roksandić, Dragutin and Radovanovic, Anita",
year = "2013",
abstract = "Background: The aim of our study was to examine the effect of mild maternal hypothyroidism on the apoptosis of the oocytes in the ovaries of rats in the early postnatal period during formation of oocytes and follicles.
Materials and Methods: Hypothyroidism was induced in pregnant and lactating rats by feeding 1.5mg/L propylthiouracil (PTU) through drinking water. Ovaries were obtained from newborns on postnatal day (PND) 0, 4 and 7. The degree of oocyte apoptosis was measured from the largest cross sections (n=2) from each ovary using by Colorimetric - TdT (terminal deoxynucleoid transferase) Enzyme in situ cell death detection kit (Merck).
Results: This preliminary result suggests that hypothyroidism induce increase of the total oocyte number from 4. day in the experimental group while the number of apoptotic oocytes is the lowest that day. However, the highest percentage of apoptosis is noticed on day 0. in control group.
Conclusion: In our further investigation we will notice whether the morphological changes induced on the oocytes appear before or after development of primordial follicles as a result of the impacts of maternal hypothyroidism on folliculogenesis of their offspring.",
publisher = "Medical University of Graz, Austria",
journal = "5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist",
title = "Apoptosis of the oocytes of hypothyroid neonatal rats",
pages = "36-36",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2737"
}

Danilović Luković, J., Marković, D., Todorović, V., Drndarević, N., Roksandić, D.,& Radovanovic, A.. (2013). Apoptosis of the oocytes of hypothyroid neonatal rats. in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist
Medical University of Graz, Austria., 36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2737

Danilović Luković J, Marković D, Todorović V, Drndarević N, Roksandić D, Radovanovic A. Apoptosis of the oocytes of hypothyroid neonatal rats. in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist. 2013;:36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2737 .

Danilović Luković, Jelena, Marković, Danica, Todorović, Vera, Drndarević, Neda, Roksandić, Dragutin, Radovanovic, Anita, "Apoptosis of the oocytes of hypothyroid neonatal rats" in 5th International Symphosium of Clinical and Applied Anatomy and 1st Paneuropian Meeting of Anatomist (2013):36-36,
https://hdl.handle.net/21.15107/rcub_rimsi_2737 .