TY  - JOUR
AU  - Radovanovic, Anita
AU  - Kovačević Filipović, Milica
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Veličkoviić, Stefan
AU  - Andrejević, Jana
AU  - Danilović Luković, Jelena
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2785
AB  - Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective
EP  - 89
IS  - 2
SP  - 80
VL  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2785
ER  - 

@article{
author = "Radovanovic, Anita and Kovačević Filipović, Milica and Milošević, Ivan and Lužajić, Tijana and Veličkoviić, Stefan and Andrejević, Jana and Danilović Luković, Jelena",
year = "2018",
abstract = "Introduction. The ovarian surface epithelium (OSE) undergoes intensive regeneration
and remodeling after each ovulation during the whole reproductive period. This process
increases the risk of one of the most common ovarian tumors in women and the female
dog. Considering the fact that maternal hypothyroidism highly impacts cell proliferation
and cell death during folliculogenesis in the early neonatal period, we aimed to analyze
its effect on OSE morphology and dynamics.
Materials and Methods. The study was performed on newborn (24-h-old) and neonatal
(4-day-old) female rats, a randomized trial between the control and hypothyroid groups,
born under controlled circumstances and hypothyroid mothers, respectively. Their
ovaries were analyzed histologically and processed to determine the OSE cell height
as an average value of four measurement points. Also, the immunopositivity of the
proliferating cell nuclear antigen (PCNA) and caspase-3 were assessed semiquantitatively.
Results and Conclusions. No major structural differences of OSE were found between
groups within the given ages except for a slight increment of OSE cell height and
incompleteness of apical cell membrane with cytoplasmic projections in hypothyroid
animals. PCNA immunopositivity of the OSE cells was higher in ovaries of hypothyroid
animals of both ages in comparison to the controls. Moreover, only scarce OSE cells
were caspase-3 positive in both groups and ages, with no difference in immunopositivity.
Our study confirms the impact of hypothyroidism in the early postnatal period onmorphology and proliferation rate of OSE cells, with no effect on caspase-3 dependent
cell removal, which may serve as a premise for future investigation of potential
carcinogenesis, in terms of prevention and treatment of ovarian cancer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective",
pages = "89-80",
number = "2",
volume = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2785"
}

Radovanovic, A., Kovačević Filipović, M., Milošević, I., Lužajić, T., Veličkoviić, S., Andrejević, J.,& Danilović Luković, J.. (2018). Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(2), 80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785

Radovanovic A, Kovačević Filipović M, Milošević I, Lužajić T, Veličkoviić S, Andrejević J, Danilović Luković J. Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective. in Veterinarski Glasnik. 2018;72(2):80-89.
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .

Radovanovic, Anita, Kovačević Filipović, Milica, Milošević, Ivan, Lužajić, Tijana, Veličkoviić, Stefan, Andrejević, Jana, Danilović Luković, Jelena, "Ovarian surface epithelium of hypothyroid newborn and neonatal rats: From PCNA and caspase-3 perspective" in Veterinarski Glasnik, 72, no. 2 (2018):80-89,
https://hdl.handle.net/21.15107/rcub_rimsi_2785 .