TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Radovanovic, Anita
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Milanović, Svetlana
AU  - Kovačević Filipović, Milica
AU  - Korać, Aleksandra
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2698
AB  - The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell.
PB  - Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade
C3  - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
T1  - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
EP  - 57
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2698
ER  - 

@conference{
author = "Danilović Luković, Jelena and Radovanovic, Anita and Milošević, Ivan and Lužajić, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra",
year = "2018",
abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell.",
publisher = "Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade",
journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade",
title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection",
pages = "57-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2698"
}

Danilović Luković, J., Radovanovic, A., Milošević, I., Lužajić, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade
Serbian Society for Mitochondrial and Free Radical Physiology Ministry of Education, Science and Technological Development University of Belgrade Faculty of Biology of University of Belgrade., 57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_2698

Danilović Luković J, Radovanovic A, Milošević I, Lužajić T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:57-57.
https://hdl.handle.net/21.15107/rcub_rimsi_2698 .

Danilović Luković, Jelena, Radovanovic, Anita, Milošević, Ivan, Lužajić, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):57-57,
https://hdl.handle.net/21.15107/rcub_rimsi_2698 .

TY  - JOUR
AU  - Milošević, Ivan
AU  - Radovanovic, Anita
AU  - Danilović Luković, Jelena
AU  - Luzajic Bozinovski, Tijana
AU  - Sourice-Petit, Sophie
AU  - Beck-Cormier, Sarah
AU  - Guicheux, Jerome
AU  - Kovačević Filipović, Milica
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2471
AB  - Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glycosaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
EP  - 320
IS  - 3
SP  - 301
VL  - 68
DO  - 10.2478/acve-2018-0026
ER  - 

@article{
author = "Milošević, Ivan and Radovanovic, Anita and Danilović Luković, Jelena and Luzajic Bozinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glycosaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
pages = "320-301",
number = "3",
volume = "68",
doi = "10.2478/acve-2018-0026"
}

Milošević, I., Radovanovic, A., Danilović Luković, J., Luzajic Bozinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J.,& Kovačević Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 68(3), 301-320.
https://doi.org/10.2478/acve-2018-0026

Milošević I, Radovanovic A, Danilović Luković J, Luzajic Bozinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Kovačević Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320.
doi:10.2478/acve-2018-0026 .

Milošević, Ivan, Radovanovic, Anita, Danilović Luković, Jelena, Luzajic Bozinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Kovačević Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320,
https://doi.org/10.2478/acve-2018-0026 . .

TY  - JOUR
AU  - Danilović Luković, Jelena
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2470
AB  - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUNEL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
EP  - 106
IS  - 1
SP  - 92
VL  - 67
DO  - 10.1515/acve-2017-0009
ER  - 

@article{
author = "Danilović Luković, Jelena",
year = "2017",
abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUNEL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats",
pages = "106-92",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0009"
}

Danilović Luković, J.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 67(1), 92-106.
https://doi.org/10.1515/acve-2017-0009

Danilović Luković J. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106.
doi:10.1515/acve-2017-0009 .

Danilović Luković, Jelena, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106,
https://doi.org/10.1515/acve-2017-0009 . .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Milošević, Sandra
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2734
AB  - Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.
PB  - Faculty of Veterinary Medicine, University of Zagreb
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
T1  - Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population
EP  - 152
SP  - 152
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2734
ER  - 

@conference{
author = "Lužajić, Tijana and Milošević, Ivan and Danilović Luković, Jelena and Marković, Danica and Milošević, Sandra and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2017",
abstract = "Thyroid hormones (TH) play a pivotal role in the development of mammalian skin, and
are necessary for both the initiation and maintenance of hair growth. Adult hypothyroid rats
showed impaired epidermal proliferation, hair growth and wound healing. Stem cells (SCs)
located at the bulges of the hair follicles are responsible for hair cycling, and contribute to
the regeneration of the new epidermis after wounding. Therefore a reduction in the number or
function of bulge stem cells could be a cause of disturbed skin reepithelization and hair follicle
maintenance. We hypothesized that a decrease in TH during prenatal development would affect
the bulge stem cell population.
Subclinical hypothyroidism was induced with propylthiouracil through drinking water
in a dose of 1.5 mg/l in pregnant Albino Oxford rats, from the first day of gravidity and during
lactation. The control group was untreated. Five, seven-day-old pups were euthanized and
skin samples were taken from the dorsal part of their bodies. The number of hair follicles was
estimated on an area of 1mm2 of dermis. For the immunohistochemical study, proliferating cell
nuclear antigen (PCNA) (Santa Cruz Biotechnology) and NANOG (Thermo Scientific) antibodies
were used. The number of PCNA and NANOG positive cells was estimated on the area of 1mm2
of hair follicle.
The number of hair follicles was reduced and the expression of PCNA was decreased
in the cells of the inner and outer sheath of hair follicles in the hypothyroid pups compared to the
controls. They also had an increased number of NANOG positive bulge cells which we presume
are multipotent SCs.
Decreased expression of PCNA demonstrates low proliferation and increased
expression of NANOG, which may be explained in two ways: 1) the inhibited differentiation of
putative SCs and/or 2) the reduction of mobilization of these cells to the epidermis or sebaceous
gland. Our results confirm that the lack of TH influences the formation of hair follicles in early
infantile rat pups.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb",
title = "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population",
pages = "152-152",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2734"
}

Lužajić, T., Milošević, I., Danilović Luković, J., Marković, D., Milošević, S., Kovačević Filipović, M.,& Radovanovic, A.. (2017). Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb
Faculty of Veterinary Medicine, University of Zagreb., 152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734

Lužajić T, Milošević I, Danilović Luković J, Marković D, Milošević S, Kovačević Filipović M, Radovanovic A. Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb. 2017;:152-152.
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .

Lužajić, Tijana, Milošević, Ivan, Danilović Luković, Jelena, Marković, Danica, Milošević, Sandra, Kovačević Filipović, Milica, Radovanovic, Anita, "Thyroid hormones affect the proliferation and/or mobilization of bulge stem cell population" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb (2017):152-152,
https://hdl.handle.net/21.15107/rcub_rimsi_2734 .

TY  - CONF
AU  - Milošević, Ivan
AU  - Danilović Luković, Jelena
AU  - Lužajić, Tijana
AU  - Milošević, Sandra
AU  - Radovanovic, Anita
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Kovačević Filipović, Milica
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2695
AB  - The effects of maternal hypothyroidism on the development and function of different
organs of their offspring are well known. It is unclear how its subclinical form affects some
of these processes, including endochondral ossification during prenatal and early postnatal
development. Our previous studies have demonstrated that hypothyroid rat pups had reduced
growth plate thickness.
The aim of the study was to assess bone turnover by defining the expression of tartrateresistant
acid phosphatase (TRAP), alkaline phosphatase tissue nonspecific isozyme (ALPL)
and mRNA for dentin matrix acidic phosphoprotein (DMP1) in the tibia of pups from subclinical
hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was
induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams
from the first day of gravidity and during lactation. The control group was untreated. Five, sevenday-
old pups from each experimental group were euthanized. All histological examinations were
performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to
assess the expression of TRAP, immunohistochemistry for ALPL and in situ hybridization for
DMP1. The area of the TRAP positive signal, optical density of ALPL immunostaining, and the
number of DMP1 positive cells were evaluated using the Image J. Expression of TRAP and ALPL
in the proximal tibial growth plate were lower in the hypothyroid pups, and the number of cells
expressing mRNA for DMP1 was decreased.
TRAP is a marker of osteoclast activity. Osteoclasts are multinucleated cells which are
necessary for bone and mineralized cartilage resorption. In osteoblasts ALPL and DMP1 are
expressed and they have an important role in the process of endochondral ossification. As we
have shown that TRAP, ALPL and DMP1 are decreased, we may conclude that maternal SHT in
rats leads to compromised cartilage to bone transition, and delayed osteogenic differentiation
in the early infantile period.
PB  - Faculty of Veterinary Medicine, University of Zagreb, Croatia
C3  - 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
T1  - Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns
EP  - 151
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2695
ER  - 

@conference{
author = "Milošević, Ivan and Danilović Luković, Jelena and Lužajić, Tijana and Milošević, Sandra and Radovanovic, Anita and Sourice-Petit, Sophie and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2017",
abstract = "The effects of maternal hypothyroidism on the development and function of different
organs of their offspring are well known. It is unclear how its subclinical form affects some
of these processes, including endochondral ossification during prenatal and early postnatal
development. Our previous studies have demonstrated that hypothyroid rat pups had reduced
growth plate thickness.
The aim of the study was to assess bone turnover by defining the expression of tartrateresistant
acid phosphatase (TRAP), alkaline phosphatase tissue nonspecific isozyme (ALPL)
and mRNA for dentin matrix acidic phosphoprotein (DMP1) in the tibia of pups from subclinical
hypothyroid dams in early postnatal development. Subclinical hypothyroidism (SHT) was
induced with propylthiouracil through drinking water (1.5 mg/l) in pregnant Albino Oxford dams
from the first day of gravidity and during lactation. The control group was untreated. Five, sevenday-
old pups from each experimental group were euthanized. All histological examinations were
performed on paraffin sections of the proximal tibial growth plate. Histochemistry was used to
assess the expression of TRAP, immunohistochemistry for ALPL and in situ hybridization for
DMP1. The area of the TRAP positive signal, optical density of ALPL immunostaining, and the
number of DMP1 positive cells were evaluated using the Image J. Expression of TRAP and ALPL
in the proximal tibial growth plate were lower in the hypothyroid pups, and the number of cells
expressing mRNA for DMP1 was decreased.
TRAP is a marker of osteoclast activity. Osteoclasts are multinucleated cells which are
necessary for bone and mineralized cartilage resorption. In osteoblasts ALPL and DMP1 are
expressed and they have an important role in the process of endochondral ossification. As we
have shown that TRAP, ALPL and DMP1 are decreased, we may conclude that maternal SHT in
rats leads to compromised cartilage to bone transition, and delayed osteogenic differentiation
in the early infantile period.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb, Croatia",
journal = "7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017",
title = "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2695"
}

Milošević, I., Danilović Luković, J., Lužajić, T., Milošević, S., Radovanovic, A., Sourice-Petit, S., Guicheux, J.,& Kovačević Filipović, M.. (2017). Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017
Faculty of Veterinary Medicine, University of Zagreb, Croatia., 151-151.
https://hdl.handle.net/21.15107/rcub_rimsi_2695

Milošević I, Danilović Luković J, Lužajić T, Milošević S, Radovanovic A, Sourice-Petit S, Guicheux J, Kovačević Filipović M. Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns. in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017. 2017;:151-151.
https://hdl.handle.net/21.15107/rcub_rimsi_2695 .

Milošević, Ivan, Danilović Luković, Jelena, Lužajić, Tijana, Milošević, Sandra, Radovanovic, Anita, Sourice-Petit, Sophie, Guicheux, Jerome, Kovačević Filipović, Milica, "Subclinical hypothyroidism in gravide albino oxford rats cause delayed osteogenic differentiation in newborns" in 7th International Congress, Veterinary Science and Profession, Zagreb, October 5th-7th 2017 (2017):151-151,
https://hdl.handle.net/21.15107/rcub_rimsi_2695 .

TY  - CONF
AU  - Danilović Luković, Jelena
AU  - Korać, Aleksandra
AU  - Milošević, Ivan
AU  - Lužajić, Tijana
AU  - Kovačević Filipović, Milica
AU  - Radovanovic, Anita
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2700
AB  - The ovarian surface epithelium (OSE) is a very dynamic part of the ovary, with an
important role in ovulation during the reproductive period. It represents a source of germ cells in
the fetal period but data regarding the role of the OSE in the postnatal period are contradictory.
Although the OSE has been the subject of research in the past few years, its ultrastructure
in newborn rats has not yet been studied. The aim of this study was to examine rat OSE
ultrastructure features in the first 24h hours after birth.
Six newborn rats were euthanized at 12h±2 hours after birth. Their ovaries were fixed in
4% glutaraldehyde, post-fixed in 1% osmium tetroxide, routinely dehydrated and embedded in
araldite. Semi-thin sections were stained with toluidine blue, and used to select areas of interest.
Ultra-thin sections of selected areas were mounted on copper grids and examined under a
Philips CM12 transmission electron microscope. Ultrastructural analysis revealed the presence
of a typically single-layered OSE with sporadic pseudostratified appearance, both consisting
of cuboidal and/or columnar cells. The OSE cells’ nuclei had irregularly dispersed chromatin,
and a central or apical location. From the surface of the OSE cells numerous microvilli and
cytoplasmic projections extended towards the peritoneal cavity. Continuous tunica albuginea
was not found, but occasionally groups of fibroblast-like cells were present, indicating the onset
of its formation. Numerous oogonia and oocytes were identified between OSE cells or up to
their basal part, suggesting that extrusion (the release of germ cells into the peritoneal cavity)
may be an important mode of germ cell elimination.
In the early postnatal period in rat ovaries massive death takes place of oogonia and
oocytes, mostly via apoptosis and autophagy. Further investigation might reveal the contribution
of extrusion as another form of germ cell elimination for both, maintaining and balancing their
number in the ovary, which is necessary for proper reproductive function.
PB  - Faculty of Veterinary Medicine, University of Zagreb, Croatia
C3  - 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb - Book of abstracts
T1  - Ovarian surface epithelium in newborn rats: germ cells warehouse
EP  - 153
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2700
ER  - 

@conference{
author = "Danilović Luković, Jelena and Korać, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Kovačević Filipović, Milica and Radovanovic, Anita",
year = "2017",
abstract = "The ovarian surface epithelium (OSE) is a very dynamic part of the ovary, with an
important role in ovulation during the reproductive period. It represents a source of germ cells in
the fetal period but data regarding the role of the OSE in the postnatal period are contradictory.
Although the OSE has been the subject of research in the past few years, its ultrastructure
in newborn rats has not yet been studied. The aim of this study was to examine rat OSE
ultrastructure features in the first 24h hours after birth.
Six newborn rats were euthanized at 12h±2 hours after birth. Their ovaries were fixed in
4% glutaraldehyde, post-fixed in 1% osmium tetroxide, routinely dehydrated and embedded in
araldite. Semi-thin sections were stained with toluidine blue, and used to select areas of interest.
Ultra-thin sections of selected areas were mounted on copper grids and examined under a
Philips CM12 transmission electron microscope. Ultrastructural analysis revealed the presence
of a typically single-layered OSE with sporadic pseudostratified appearance, both consisting
of cuboidal and/or columnar cells. The OSE cells’ nuclei had irregularly dispersed chromatin,
and a central or apical location. From the surface of the OSE cells numerous microvilli and
cytoplasmic projections extended towards the peritoneal cavity. Continuous tunica albuginea
was not found, but occasionally groups of fibroblast-like cells were present, indicating the onset
of its formation. Numerous oogonia and oocytes were identified between OSE cells or up to
their basal part, suggesting that extrusion (the release of germ cells into the peritoneal cavity)
may be an important mode of germ cell elimination.
In the early postnatal period in rat ovaries massive death takes place of oogonia and
oocytes, mostly via apoptosis and autophagy. Further investigation might reveal the contribution
of extrusion as another form of germ cell elimination for both, maintaining and balancing their
number in the ovary, which is necessary for proper reproductive function.",
publisher = "Faculty of Veterinary Medicine, University of Zagreb, Croatia",
journal = "7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb - Book of abstracts",
title = "Ovarian surface epithelium in newborn rats: germ cells warehouse",
pages = "153-153",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2700"
}

Danilović Luković, J., Korać, A., Milošević, I., Lužajić, T., Kovačević Filipović, M.,& Radovanovic, A.. (2017). Ovarian surface epithelium in newborn rats: germ cells warehouse. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb - Book of abstracts
Faculty of Veterinary Medicine, University of Zagreb, Croatia., 153-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2700

Danilović Luković J, Korać A, Milošević I, Lužajić T, Kovačević Filipović M, Radovanovic A. Ovarian surface epithelium in newborn rats: germ cells warehouse. in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb - Book of abstracts. 2017;:153-153.
https://hdl.handle.net/21.15107/rcub_rimsi_2700 .

Danilović Luković, Jelena, Korać, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Kovačević Filipović, Milica, Radovanovic, Anita, "Ovarian surface epithelium in newborn rats: germ cells warehouse" in 7th International Congress Veterinary science and proffesion, October 5th-7th 2017, Zagreb - Book of abstracts (2017):153-153,
https://hdl.handle.net/21.15107/rcub_rimsi_2700 .

TY  - JOUR
AU  - Roksandić, Dragutin
AU  - Radovanovic, Anita
AU  - Danilović Luković, Jelena
AU  - Marković, Danica
AU  - Kovačević Filipović, Milica
AU  - Čolić, Miodrag
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2469
AB  - The purpose of this work was to investigate the infl uence of hypothyroidism on
spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats.
Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil
(PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of
lactation. Fourteen-day-old pups were sacrifi ced and the thyroid-stimulating hormone
(TSH) serum concentration and thyroid activation index (Ia) were determined.
Increased serum level of TSH and increased Ia showed that pups from PTU treated
mothers were hypothyroid. White and red spleen pulp, marginal zone and connective
tissue volume density has been assessed by using the stereological method. Using
immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes
and CD68+ macrophages were quantifi ed. A signifi cant reduction of volume density
of the periarteriolar lymphocyte sheath (VvPALS) and lymphatic follicles (Vvf) due
to depletion of T and B lymphocytes respectively, was observed in the spleens of
hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp),
marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the
number of CD68+ macrophages in the spleens of hypothyroid pups compared to
controls. These results indicate that thyroid hormones might be important for normal
development of both, specifi c and innate immune cells in the spleen during prenatal
and early postnatal period.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
T2  - Acta Veterinaria-Beograd
T1  - Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats
EP  - 259
IS  - 2
SP  - 246
VL  - 65
DO  - 10.1515/acve-2015-0021
ER  - 

@article{
author = "Roksandić, Dragutin and Radovanovic, Anita and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Čolić, Miodrag",
year = "2015",
abstract = "The purpose of this work was to investigate the infl uence of hypothyroidism on
spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats.
Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil
(PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of
lactation. Fourteen-day-old pups were sacrifi ced and the thyroid-stimulating hormone
(TSH) serum concentration and thyroid activation index (Ia) were determined.
Increased serum level of TSH and increased Ia showed that pups from PTU treated
mothers were hypothyroid. White and red spleen pulp, marginal zone and connective
tissue volume density has been assessed by using the stereological method. Using
immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes
and CD68+ macrophages were quantifi ed. A signifi cant reduction of volume density
of the periarteriolar lymphocyte sheath (VvPALS) and lymphatic follicles (Vvf) due
to depletion of T and B lymphocytes respectively, was observed in the spleens of
hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp),
marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the
number of CD68+ macrophages in the spleens of hypothyroid pups compared to
controls. These results indicate that thyroid hormones might be important for normal
development of both, specifi c and innate immune cells in the spleen during prenatal
and early postnatal period.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats",
pages = "259-246",
number = "2",
volume = "65",
doi = "10.1515/acve-2015-0021"
}

Roksandić, D., Radovanovic, A., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Čolić, M.. (2015). Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 65(2), 246-259.
https://doi.org/10.1515/acve-2015-0021

Roksandić D, Radovanovic A, Danilović Luković J, Marković D, Kovačević Filipović M, Čolić M. Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd. 2015;65(2):246-259.
doi:10.1515/acve-2015-0021 .

Roksandić, Dragutin, Radovanovic, Anita, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Čolić, Miodrag, "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats" in Acta Veterinaria-Beograd, 65, no. 2 (2015):246-259,
https://doi.org/10.1515/acve-2015-0021 . .

TY  - CONF
AU  - Danilović Luković, Jelena
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2517
AB  - Serum iron (Fe) deficiency is a common problem in newborn calves in dairy industry. Iron deficiency have an influence on the immune status of calves as well as on erythropoesis and thrombopoesis. Therefore, the aim of this study was to determine Fe status in neonatal calves and to relate Fe status to basic hematological and biochemical parameters and serum fibrinogen levels. Blood was collected from 21 calves of Holstein-Friesian breed (7 female and 14 male) 12 to 24 hours after birth. Calves were divided into two groups. Group A included calves with iron concentration less than 14μmol/L and group B calves with concentration greater than14μmol/L. Iron concentration, total iron binding capacity (TIBC), fibrinogen, albumin and total protein were determined using standard laboratory techniques. Leukocyte count was determined using manual and PCV using microhematocrit method. The platelet count and differential leukocyte count was obtained by blood smears examination. The results have shown that all animals at birth had iron deficiency without signs of anemia. Platelet count was higher in group B. TIBC was significantly higher and transferrin saturation was significantly lower in group A. Fibrinogen concentration, other blood elements and globulin concentration were within the previously reported values for neonatal calves. Based on these results, we supposed that concentration of iron can influence platelet count in healthy neonatal cattle. Further studies are needed to confirm these findings and to establish its physiological significance.
PB  - Serbian Buiatrics' Association, Faculty of Veterinary Medicine, University of Belgrade
C3  - XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd
T1  - Hematology and biochemistry parametars in neonatal calves with low iron levels
EP  - 288
SP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2517
ER  - 

@conference{
author = "Danilović Luković, Jelena",
year = "2013",
abstract = "Serum iron (Fe) deficiency is a common problem in newborn calves in dairy industry. Iron deficiency have an influence on the immune status of calves as well as on erythropoesis and thrombopoesis. Therefore, the aim of this study was to determine Fe status in neonatal calves and to relate Fe status to basic hematological and biochemical parameters and serum fibrinogen levels. Blood was collected from 21 calves of Holstein-Friesian breed (7 female and 14 male) 12 to 24 hours after birth. Calves were divided into two groups. Group A included calves with iron concentration less than 14μmol/L and group B calves with concentration greater than14μmol/L. Iron concentration, total iron binding capacity (TIBC), fibrinogen, albumin and total protein were determined using standard laboratory techniques. Leukocyte count was determined using manual and PCV using microhematocrit method. The platelet count and differential leukocyte count was obtained by blood smears examination. The results have shown that all animals at birth had iron deficiency without signs of anemia. Platelet count was higher in group B. TIBC was significantly higher and transferrin saturation was significantly lower in group A. Fibrinogen concentration, other blood elements and globulin concentration were within the previously reported values for neonatal calves. Based on these results, we supposed that concentration of iron can influence platelet count in healthy neonatal cattle. Further studies are needed to confirm these findings and to establish its physiological significance.",
publisher = "Serbian Buiatrics' Association, Faculty of Veterinary Medicine, University of Belgrade",
journal = "XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd",
title = "Hematology and biochemistry parametars in neonatal calves with low iron levels",
pages = "288-284",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2517"
}

Danilović Luković, J.. (2013). Hematology and biochemistry parametars in neonatal calves with low iron levels. in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd
Serbian Buiatrics' Association, Faculty of Veterinary Medicine, University of Belgrade., 284-288.
https://hdl.handle.net/21.15107/rcub_rimsi_2517

Danilović Luković J. Hematology and biochemistry parametars in neonatal calves with low iron levels. in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd. 2013;:284-288.
https://hdl.handle.net/21.15107/rcub_rimsi_2517 .

Danilović Luković, Jelena, "Hematology and biochemistry parametars in neonatal calves with low iron levels" in XIII Srednjeevropski Kongres Bujatričara/XIII Middle European Buiatric's Congress, Beograd (2013):284-288,
https://hdl.handle.net/21.15107/rcub_rimsi_2517 .

TY  - CONF
AU  - Lužajić, Tijana
AU  - Katić, Marko
AU  - Danilović Luković, Jelena
AU  - Blond, Bojan
AU  - Bogdanović, Dragan
AU  - Kovačević Filipović, Milica
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2502
AB  - Although extensive data are available on leukocyte changes in prepartal period in cows with
and without retained placenta, there is no data describing changes in platelet count. The aim of
this study was to define changes in platelet count in cows, with and without retained placenta,
three weeks before and one day after calving. Using fibrinogen level as a general reference to
cow's health, we also tested the hypothesis that cows, that develop placenta retention in
prepartal period, have a subclinical systemic inflammatory reaction. Blood samples taken from
14 Holstein cows were analyzed, three times in a week interval before and 24 hours after
calving. After calving, cows were retrospectively divided, in the group without (n=9) and the
group with retained placenta (n=5). Clauss method was used for determination of fibrinogen
concentration and blood smear examination for assessment of platelet count. The results have
shown that retained placenta is associated with increase in platelet count seven days before
calving and decrease in platelet count 24 hours after calving comparing to precalving values.
The week before calving group of cows, that developed retained placenta had platelet count
significantly higher, comparing to control group of cows. Fibrinogen level was not significantly
different between groups. The recorded increase in platelet count, one week before calving can
be related to unidentified stress factors, and decrease of platelet count 24 hours after calving,
can be explained by their increased consumption, due to activation of haemostatic mechanisms
after placenta abruption.
PB  - Faculty of Veterinary Medicine, University of Belgrade, Serbia
C3  - XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013
T1  - Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta
EP  - 441
SP  - 437
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2502
ER  - 

@conference{
author = "Lužajić, Tijana and Katić, Marko and Danilović Luković, Jelena and Blond, Bojan and Bogdanović, Dragan and Kovačević Filipović, Milica",
year = "2013",
abstract = "Although extensive data are available on leukocyte changes in prepartal period in cows with
and without retained placenta, there is no data describing changes in platelet count. The aim of
this study was to define changes in platelet count in cows, with and without retained placenta,
three weeks before and one day after calving. Using fibrinogen level as a general reference to
cow's health, we also tested the hypothesis that cows, that develop placenta retention in
prepartal period, have a subclinical systemic inflammatory reaction. Blood samples taken from
14 Holstein cows were analyzed, three times in a week interval before and 24 hours after
calving. After calving, cows were retrospectively divided, in the group without (n=9) and the
group with retained placenta (n=5). Clauss method was used for determination of fibrinogen
concentration and blood smear examination for assessment of platelet count. The results have
shown that retained placenta is associated with increase in platelet count seven days before
calving and decrease in platelet count 24 hours after calving comparing to precalving values.
The week before calving group of cows, that developed retained placenta had platelet count
significantly higher, comparing to control group of cows. Fibrinogen level was not significantly
different between groups. The recorded increase in platelet count, one week before calving can
be related to unidentified stress factors, and decrease of platelet count 24 hours after calving,
can be explained by their increased consumption, due to activation of haemostatic mechanisms
after placenta abruption.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013",
title = "Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta",
pages = "441-437",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2502"
}

Lužajić, T., Katić, M., Danilović Luković, J., Blond, B., Bogdanović, D.,& Kovačević Filipović, M.. (2013). Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta. in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013
Faculty of Veterinary Medicine, University of Belgrade, Serbia., 437-441.
https://hdl.handle.net/21.15107/rcub_rimsi_2502

Lužajić T, Katić M, Danilović Luković J, Blond B, Bogdanović D, Kovačević Filipović M. Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta. in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013. 2013;:437-441.
https://hdl.handle.net/21.15107/rcub_rimsi_2502 .

Lužajić, Tijana, Katić, Marko, Danilović Luković, Jelena, Blond, Bojan, Bogdanović, Dragan, Kovačević Filipović, Milica, "Prepartal platelet count and fibrinogen concentration in dairy cows with and without retained placenta" in XIII Middle European Buiatric's Congress, Belgrade, June 5-8, 2013 (2013):437-441,
https://hdl.handle.net/21.15107/rcub_rimsi_2502 .