Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
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Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle
assembly and premature follicular atresia with ovary failure. This work investigates the
infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on
the number and morphology of oogonia/oocytes in newborn rat pups with light and
transmission electron microscopy. Expression of apoptosis and autophagy markers in
oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn
pups had a decreased number of mitotic and resting oogonia, while the number of
altered oogonia/oocytes was increased. Ultrastructural observations revealed the
increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes
undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group.
The most abundant altered oogonia/oocytes in the hypothyroid group were those with
morphological features of advanced autophagy and Z-cells. The percentage of TUN...EL
(terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/
oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was
recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these
proteins were not involved in the oogonia/oocyte alteration process during prenatal
rat hypothyroidism. The obtained results indicate that developmental hypothyroidism
in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the
advanced process of autophagy.
Кључне речи:
ATG7 / caspase-3 / LC3 / meiosis prophase I / secondary necrosisИзвор:
Acta Veterinaria-Beograd, 2017, 67, 1, 92-106Издавач:
- Faculty of Veterinary Medicine, University of Belgrade, Serbia
Финансирање / пројекти:
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Danilović Luković, Jelena PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2470 AB - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy. PB - Faculty of Veterinary Medicine, University of Belgrade, Serbia T2 - Acta Veterinaria-Beograd T1 - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats EP - 106 IS - 1 SP - 92 VL - 67 DO - 10.1515/acve-2017-0009 ER -
@article{ author = "Danilović Luković, Jelena", year = "2017", abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the infl uence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was signifi cantly lower in the hypothyroid group. No signifi cant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly refl ecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy.", publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia", journal = "Acta Veterinaria-Beograd", title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats", pages = "106-92", number = "1", volume = "67", doi = "10.1515/acve-2017-0009" }
Danilović Luković, J.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd Faculty of Veterinary Medicine, University of Belgrade, Serbia., 67(1), 92-106. https://doi.org/10.1515/acve-2017-0009
Danilović Luković J. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106. doi:10.1515/acve-2017-0009 .
Danilović Luković, Jelena, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106, https://doi.org/10.1515/acve-2017-0009 . .