Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats
Аутори
Roksandić, DragutinRadovanovic, Anita
Danilović Luković, Jelena
Marković, Danica
Kovačević Filipović, Milica
Čolić, Miodrag
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The purpose of this work was to investigate the infl uence of hypothyroidism on
spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats.
Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil
(PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of
lactation. Fourteen-day-old pups were sacrifi ced and the thyroid-stimulating hormone
(TSH) serum concentration and thyroid activation index (Ia) were determined.
Increased serum level of TSH and increased Ia showed that pups from PTU treated
mothers were hypothyroid. White and red spleen pulp, marginal zone and connective
tissue volume density has been assessed by using the stereological method. Using
immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes
and CD68+ macrophages were quantifi ed. A signifi cant reduction of volume density
of the periarteriolar lymphocyte sheath (VvPALS) and lymphatic follicles (Vvf) due
to depletion ...of T and B lymphocytes respectively, was observed in the spleens of
hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp),
marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the
number of CD68+ macrophages in the spleens of hypothyroid pups compared to
controls. These results indicate that thyroid hormones might be important for normal
development of both, specifi c and innate immune cells in the spleen during prenatal
and early postnatal period.
Кључне речи:
spleen / hypothyroidism / stereology / immunohistochemistry / ratИзвор:
Acta Veterinaria-Beograd, 2015, 65, 2, 246-259Издавач:
- Faculty of Veterinary Medicine, University of Belgrade, Serbia
Финансирање / пројекти:
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Roksandić, Dragutin AU - Radovanovic, Anita AU - Danilović Luković, Jelena AU - Marković, Danica AU - Kovačević Filipović, Milica AU - Čolić, Miodrag PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2469 AB - The purpose of this work was to investigate the infl uence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrifi ced and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantifi ed. A signifi cant reduction of volume density of the periarteriolar lymphocyte sheath (VvPALS) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specifi c and innate immune cells in the spleen during prenatal and early postnatal period. PB - Faculty of Veterinary Medicine, University of Belgrade, Serbia T2 - Acta Veterinaria-Beograd T1 - Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats EP - 259 IS - 2 SP - 246 VL - 65 DO - 10.1515/acve-2015-0021 ER -
@article{ author = "Roksandić, Dragutin and Radovanovic, Anita and Danilović Luković, Jelena and Marković, Danica and Kovačević Filipović, Milica and Čolić, Miodrag", year = "2015", abstract = "The purpose of this work was to investigate the infl uence of hypothyroidism on spleen tissue morphology and immune cell density in fourteen-day-old juvenile rats. Hypothyroidism in pups (n=10) was induced by administration of propylthiouracil (PTU) in drinking water (1.5 mg/L) to their mothers during pregnancy and period of lactation. Fourteen-day-old pups were sacrifi ced and the thyroid-stimulating hormone (TSH) serum concentration and thyroid activation index (Ia) were determined. Increased serum level of TSH and increased Ia showed that pups from PTU treated mothers were hypothyroid. White and red spleen pulp, marginal zone and connective tissue volume density has been assessed by using the stereological method. Using immunohistochemistry, the present CD3+ T lymphocytes, CD45RA+ B lymphocytes and CD68+ macrophages were quantifi ed. A signifi cant reduction of volume density of the periarteriolar lymphocyte sheath (VvPALS) and lymphatic follicles (Vvf) due to depletion of T and B lymphocytes respectively, was observed in the spleens of hypothyroid pups compared to controls. The volume density of the red pulp (Vvrp), marginal zone (Vvmz) and connective tissue (Vvct) was increased, as well as the number of CD68+ macrophages in the spleens of hypothyroid pups compared to controls. These results indicate that thyroid hormones might be important for normal development of both, specifi c and innate immune cells in the spleen during prenatal and early postnatal period.", publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia", journal = "Acta Veterinaria-Beograd", title = "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats", pages = "259-246", number = "2", volume = "65", doi = "10.1515/acve-2015-0021" }
Roksandić, D., Radovanovic, A., Danilović Luković, J., Marković, D., Kovačević Filipović, M.,& Čolić, M.. (2015). Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd Faculty of Veterinary Medicine, University of Belgrade, Serbia., 65(2), 246-259. https://doi.org/10.1515/acve-2015-0021
Roksandić D, Radovanovic A, Danilović Luković J, Marković D, Kovačević Filipović M, Čolić M. Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats. in Acta Veterinaria-Beograd. 2015;65(2):246-259. doi:10.1515/acve-2015-0021 .
Roksandić, Dragutin, Radovanovic, Anita, Danilović Luković, Jelena, Marković, Danica, Kovačević Filipović, Milica, Čolić, Miodrag, "Stereological and immunohistochemical study of the spleen in hypothyroid juvenile rats" in Acta Veterinaria-Beograd, 65, no. 2 (2015):246-259, https://doi.org/10.1515/acve-2015-0021 . .