TY  - JOUR
AU  - Janković, Tamara
AU  - Danilović Luković, Jelena
AU  - Miler, Irena
AU  - Mitic, Ninoslav
AU  - Hajduković, Ljiljana
AU  - Janković, Miroslava
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2464
AB  - Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct
tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of
different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal
proteins were determined aiming at the solubilization signature as an intrinsic multimolecular
marker and a new parameter suitable as a reference for the comparison of EVs populations in health and
disease.
Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that
maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase
(GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission
electron microscopy, electrophoresis, and lectin blot.
Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count
revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly
part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral
membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns
mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/
GGT/concanavalin A-reactive glycoproteins.
Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the
surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular
environment, both of which contribute to the composition of spatial membrane arrangements.
PB  - Upsala Medical Society
T2  - Upsala journal of medical sciences
T1  - Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men
VL  - 126
DO  - 10.48101/ujms.v126.7673
ER  - 

@article{
author = "Janković, Tamara and Danilović Luković, Jelena and Miler, Irena and Mitic, Ninoslav and Hajduković, Ljiljana and Janković, Miroslava",
year = "2021",
abstract = "Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct
tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of
different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal
proteins were determined aiming at the solubilization signature as an intrinsic multimolecular
marker and a new parameter suitable as a reference for the comparison of EVs populations in health and
disease.
Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that
maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase
(GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission
electron microscopy, electrophoresis, and lectin blot.
Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count
revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly
part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral
membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns
mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/
GGT/concanavalin A-reactive glycoproteins.
Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the
surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular
environment, both of which contribute to the composition of spatial membrane arrangements.",
publisher = "Upsala Medical Society",
journal = "Upsala journal of medical sciences",
title = "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men",
volume = "126",
doi = "10.48101/ujms.v126.7673"
}

Janković, T., Danilović Luković, J., Miler, I., Mitic, N., Hajduković, L.,& Janković, M.. (2021). Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences
Upsala Medical Society., 126.
https://doi.org/10.48101/ujms.v126.7673

Janković T, Danilović Luković J, Miler I, Mitic N, Hajduković L, Janković M. Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences. 2021;126.
doi:10.48101/ujms.v126.7673 .

Janković, Tamara, Danilović Luković, Jelena, Miler, Irena, Mitic, Ninoslav, Hajduković, Ljiljana, Janković, Miroslava, "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men" in Upsala journal of medical sciences, 126 (2021),
https://doi.org/10.48101/ujms.v126.7673 . .