Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana
Аутори
Vojvodić, SnežanaStanić, Marina
Danilović Luković, Jelena
Milivojević, Marija
Zechmann, Bernd
Dimitrijević, Milena
Opačić, Miloš
Pittman, Jon K.
Spasojević, Ivan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher... capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio
Кључне речи:
microalgae / metals / ROS / mucilage polymersИзвор:
FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 2022, 747-748Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Vojvodić, Snežana AU - Stanić, Marina AU - Danilović Luković, Jelena AU - Milivojević, Marija AU - Zechmann, Bernd AU - Dimitrijević, Milena AU - Opačić, Miloš AU - Pittman, Jon K. AU - Spasojević, Ivan PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2403 AB - METHODS The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed by oxidation-sensitive fluorescent probe and assays for thiols. RESULTS Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and 24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn in relation to higher quotas than Ni that are required for normal functio C3 - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. T1 - Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana EP - 748 SP - 747 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2403 ER -
@conference{ author = "Vojvodić, Snežana and Stanić, Marina and Danilović Luković, Jelena and Milivojević, Marija and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Pittman, Jon K. and Spasojević, Ivan", year = "2022", abstract = "METHODS The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed by oxidation-sensitive fluorescent probe and assays for thiols. RESULTS Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and 24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn in relation to higher quotas than Ni that are required for normal functio", journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.", title = "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana", pages = "748-747", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2403" }
Vojvodić, S., Stanić, M., Danilović Luković, J., Milivojević, M., Zechmann, B., Dimitrijević, M., Opačić, M., Pittman, J. K.,& Spasojević, I.. (2022). Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 747-748. https://hdl.handle.net/21.15107/rcub_rimsi_2403
Vojvodić S, Stanić M, Danilović Luković J, Milivojević M, Zechmann B, Dimitrijević M, Opačić M, Pittman JK, Spasojević I. Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:747-748. https://hdl.handle.net/21.15107/rcub_rimsi_2403 .
Vojvodić, Snežana, Stanić, Marina, Danilović Luković, Jelena, Milivojević, Marija, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Pittman, Jon K., Spasojević, Ivan, "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):747-748, https://hdl.handle.net/21.15107/rcub_rimsi_2403 .