The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study
Аутори
Danilović Luković, JelenaZechmann, Bernd
Vojvodić, Snežana
Bogdanović Pristov, Jelena
Stanić, Marina
Pittman, Jon K.
Spasojević, Ivan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron
translucent line inserted between two electron dense lines; the outermost layer is a mature mother
wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the
obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of
TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response
to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae
exposed to 5 Gy was not significantly different than in controls. A similar trend was
observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that
no effects of radiation on biomass, at doses applied here, could be observed.
Кључне речи:
microalgae / ionizing radiation / cell wall / electron microscopyИзвор:
14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia, 2019, 152-153Издавач:
- Institute for Biological Research “Siniša Stanković” and others contributors
Финансирање / пројекти:
- NATO Science for Peace and Security Programme - G5320
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Danilović Luković, Jelena AU - Zechmann, Bernd AU - Vojvodić, Snežana AU - Bogdanović Pristov, Jelena AU - Stanić, Marina AU - Pittman, Jon K. AU - Spasojević, Ivan PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2408 AB - Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron translucent line inserted between two electron dense lines; the outermost layer is a mature mother wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae exposed to 5 Gy was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed. PB - Institute for Biological Research “Siniša Stanković” and others contributors C3 - 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia T1 - The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study EP - 153 SP - 152 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2408 ER -
@conference{ author = "Danilović Luković, Jelena and Zechmann, Bernd and Vojvodić, Snežana and Bogdanović Pristov, Jelena and Stanić, Marina and Pittman, Jon K. and Spasojević, Ivan", year = "2019", abstract = "Cell wall of C. sorokiniana is composed of trilaminar sheath (TLS electron translucent line inserted between two electron dense lines; the outermost layer is a mature mother wall, while the thin inner layer is a daughter wall), and fibrilar cell wall. It is noteworthy that the obtained diameters for untreated microalgae were in accordance with available data. The analysis of TEM micrographs showed that there were no significant changes in the thickness of TLS for any of the used doses. However, the diameter of fibrillar wall was increased in response to irradiation for microalgae exposed to 1 Gy and 2 Gy. The thickness of cell wall in microalgae exposed to 5 Gy was not significantly different than in controls. A similar trend was observed by gravimetry of dry cell wall isolates normalized to biomass. It is important to note that no effects of radiation on biomass, at doses applied here, could be observed.", publisher = "Institute for Biological Research “Siniša Stanković” and others contributors", journal = "14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia", title = "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study", pages = "153-152", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2408" }
Danilović Luković, J., Zechmann, B., Vojvodić, S., Bogdanović Pristov, J., Stanić, M., Pittman, J. K.,& Spasojević, I.. (2019). The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia Institute for Biological Research “Siniša Stanković” and others contributors., 152-153. https://hdl.handle.net/21.15107/rcub_rimsi_2408
Danilović Luković J, Zechmann B, Vojvodić S, Bogdanović Pristov J, Stanić M, Pittman JK, Spasojević I. The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study. in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia. 2019;:152-153. https://hdl.handle.net/21.15107/rcub_rimsi_2408 .
Danilović Luković, Jelena, Zechmann, Bernd, Vojvodić, Snežana, Bogdanović Pristov, Jelena, Stanić, Marina, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the cell wall of microalgae Chlorella sorokiniana – TEM study" in 14th Multinational Congress on Microscopy. September 15–20, 2019, Belgrade, Serbia (2019):152-153, https://hdl.handle.net/21.15107/rcub_rimsi_2408 .