TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1725
AB  - Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1725
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1725"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

TY  - CONF
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Perošević-Bajčeta, Ana
AU  - Joksimović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5726
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2172
AB  - More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.
PB  - Institute of Oceanography and Fisheries
C3  - 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
T1  - Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2172
ER  - 

@conference{
author = "Martinović, Rajko and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Gačić, Zoran and Vuković-Gačić, Branka and Perošević-Bajčeta, Ana and Joksimović, Danijela",
year = "2022",
abstract = "More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.",
publisher = "Institute of Oceanography and Fisheries",
journal = "2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries",
title = "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2172"
}

Martinović, R., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Gačić, Z., Vuković-Gačić, B., Perošević-Bajčeta, A.,& Joksimović, D.. (2022). Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
Institute of Oceanography and Fisheries., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172

Martinović R, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Gačić Z, Vuković-Gačić B, Perošević-Bajčeta A, Joksimović D. Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries. 2022;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

Martinović, Rajko, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Gačić, Zoran, Vuković-Gačić, Branka, Perošević-Bajčeta, Ana, Joksimović, Danijela, "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast" in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries (2022):33,
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

TY  - JOUR
AU  - Martinović, Rajko
AU  - Joksimović, Danijela
AU  - García-March, José Rafael
AU  - Vicente, Nardo
AU  - Gačić, Zoran
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1573
AB  - A non-invasive laser fiber-optic method based on infrared sensors for heart rate (Hr) recording was applied to assess the physiological condition of Pinna nobilis. During 2017, the specimens of P. nobilis were sampled at three sites within the Boka Kotorska Bay, Montenegro and used for ex situ experiments with short-term reduction/restoration of ambient salinity to evaluate their physiological adaptive capacity based on heart rate recovery time (Trec). Mean Trec for specimens from Sv. Nedjelja (reference site), Dobrota and Sv. Stasije were 72 ± 3, 91 ± 7 and 117 ± 15 min, while the coefficients of variation (CV) were 0.12, 0.13 and 0.17, respectively. Resting heart rate (Hrrest) and Trec showed statistically significant differences between the groups of mussels from Dobrota and Sv. Stasije in comparison to the reference site. Statistically significant correlations were observed between Trec and shell length/width, which was not the case in comparison between Hrrest and shell length/width. The lower adaptive capacity within the P. nobilis specimens from Dobrota and Sv. Stasije in comparison to the reference site could occur due to stress induced by deterioration of environmental conditions, which could have led to impairment of the physiological state of the mussels evaluated by Hr. All the specimens of P. nobilis survived the experimental treatments; afterwards, they were successfully transplanted at the Dobrota site. The experimental unit with sensor technology applied in this study can provide Hr recording in real time and could have an application in monitoring the physiological/health state of P. nobilis individuals maintained in aquaria.
PB  - MDPI
T2  - Micromachines
T1  - Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test
IS  - 9
SP  - 1549
VL  - 13
DO  - 10.3390/mi13091549
ER  - 

@article{
author = "Martinović, Rajko and Joksimović, Danijela and García-March, José Rafael and Vicente, Nardo and Gačić, Zoran",
year = "2022",
abstract = "A non-invasive laser fiber-optic method based on infrared sensors for heart rate (Hr) recording was applied to assess the physiological condition of Pinna nobilis. During 2017, the specimens of P. nobilis were sampled at three sites within the Boka Kotorska Bay, Montenegro and used for ex situ experiments with short-term reduction/restoration of ambient salinity to evaluate their physiological adaptive capacity based on heart rate recovery time (Trec). Mean Trec for specimens from Sv. Nedjelja (reference site), Dobrota and Sv. Stasije were 72 ± 3, 91 ± 7 and 117 ± 15 min, while the coefficients of variation (CV) were 0.12, 0.13 and 0.17, respectively. Resting heart rate (Hrrest) and Trec showed statistically significant differences between the groups of mussels from Dobrota and Sv. Stasije in comparison to the reference site. Statistically significant correlations were observed between Trec and shell length/width, which was not the case in comparison between Hrrest and shell length/width. The lower adaptive capacity within the P. nobilis specimens from Dobrota and Sv. Stasije in comparison to the reference site could occur due to stress induced by deterioration of environmental conditions, which could have led to impairment of the physiological state of the mussels evaluated by Hr. All the specimens of P. nobilis survived the experimental treatments; afterwards, they were successfully transplanted at the Dobrota site. The experimental unit with sensor technology applied in this study can provide Hr recording in real time and could have an application in monitoring the physiological/health state of P. nobilis individuals maintained in aquaria.",
publisher = "MDPI",
journal = "Micromachines",
title = "Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test",
number = "9",
pages = "1549",
volume = "13",
doi = "10.3390/mi13091549"
}

Martinović, R., Joksimović, D., García-March, J. R., Vicente, N.,& Gačić, Z.. (2022). Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test. in Micromachines
MDPI., 13(9), 1549.
https://doi.org/10.3390/mi13091549

Martinović R, Joksimović D, García-March JR, Vicente N, Gačić Z. Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test. in Micromachines. 2022;13(9):1549.
doi:10.3390/mi13091549 .

Martinović, Rajko, Joksimović, Danijela, García-March, José Rafael, Vicente, Nardo, Gačić, Zoran, "Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test" in Micromachines, 13, no. 9 (2022):1549,
https://doi.org/10.3390/mi13091549 . .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1688
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.
PB  - Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany
C3  - 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1688
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.",
publisher = "Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany",
journal = "43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1688"
}

Đorđević Aleksić, J., Kostić-Vuković, J., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany..
https://hdl.handle.net/21.15107/rcub_rimsi_1688

Đorđević Aleksić J, Kostić-Vuković J, Sunjog K, Višnjić Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;.
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

Đorđević Aleksić, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods." in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1501
AB  - This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 193(8).
https://doi.org/10.1007/s10661-021-09232-6

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Gačić Z, Lenhardt M, Vukovic-Gacic B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8).
doi:10.1007/s10661-021-09232-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021),
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - CHAP
AU  - Martinović, Rajko
AU  - Petović, S.
AU  - Joksimović, D.
AU  - Bunet, R.
AU  - Couvray, S.
AU  - Kirchhofer, D.
AU  - Simide, R.
AU  - Garcia-March, J.R.
AU  - Tena-Medialdea, J.
AU  - Castelli, A.
AU  - Gačić, Zoran
AU  - Bonnefont, J.-L.
AU  - Vicente, N.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1432
AB  - In this study, the comparative results of the fan mussel Pinna nobilis larvae collection during 3 years (2016–2019) on the sites of the Montenegrin Adriatic coast and the Western Mediterranean, France are presented. In both countries, growth studies of collected fan mussel juveniles were carried out as well. After 1 year growth measurement of P. nobilis recruits in their natural habitat on the site Dobrota, Montenegro, mean shell length was 198.58 ± 17.77 mm for the recruits from Sv. Nedjelja and 206.73 ± 16.40 mm for the recruits collected from Ljuta. The growth study carried out in a laboratory tank in France indicated that the mean shell length of P. nobilis recruits after a 9 month period was 100.50 ± 7.59 mm for the recruits from Bomasse, 96.33 ± 11.06 mm from Basse Renette 1, and 95.75 ± 8.45 mm for recruits from Basse Renette 2, respectively. In spite of much larger mean shell lengths obtained in Montenegro, mean monthly growth rate of P. nobilis recruits bred in France was higher due to more stable conditions and access to food within the tank in contrast to variations of environmental parameters in their natural habitat. We have presented first data on P. nobilis recruitment and growth in Montenegro and showed higher growth rate in comparison with the other sites in the Adriatic Sea and Mediterranean. It was shown that the temperature is of high importance for the growth rate of P. nobilis juveniles in their natural habitat due to lower growth during winter. Anthropogenic pressure was the main obstacle for development of P. nobilis populations during the study period, while biological pollution as the main threat for P. nobilis survival will be the subject of further studies in the Montenegrin Adriatic coast.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Recruitment and Growth of the Fan Mussel Pinna nobilis in the Montenegrin Adriatic Coast and Comparison with the Western Mediterranean
EP  - 213
SP  - 193
VL  - 109
DO  - 10.1007/698_2021_749
ER  - 

@inbook{
author = "Martinović, Rajko and Petović, S. and Joksimović, D. and Bunet, R. and Couvray, S. and Kirchhofer, D. and Simide, R. and Garcia-March, J.R. and Tena-Medialdea, J. and Castelli, A. and Gačić, Zoran and Bonnefont, J.-L. and Vicente, N.",
year = "2021",
abstract = "In this study, the comparative results of the fan mussel Pinna nobilis larvae collection during 3 years (2016–2019) on the sites of the Montenegrin Adriatic coast and the Western Mediterranean, France are presented. In both countries, growth studies of collected fan mussel juveniles were carried out as well. After 1 year growth measurement of P. nobilis recruits in their natural habitat on the site Dobrota, Montenegro, mean shell length was 198.58 ± 17.77 mm for the recruits from Sv. Nedjelja and 206.73 ± 16.40 mm for the recruits collected from Ljuta. The growth study carried out in a laboratory tank in France indicated that the mean shell length of P. nobilis recruits after a 9 month period was 100.50 ± 7.59 mm for the recruits from Bomasse, 96.33 ± 11.06 mm from Basse Renette 1, and 95.75 ± 8.45 mm for recruits from Basse Renette 2, respectively. In spite of much larger mean shell lengths obtained in Montenegro, mean monthly growth rate of P. nobilis recruits bred in France was higher due to more stable conditions and access to food within the tank in contrast to variations of environmental parameters in their natural habitat. We have presented first data on P. nobilis recruitment and growth in Montenegro and showed higher growth rate in comparison with the other sites in the Adriatic Sea and Mediterranean. It was shown that the temperature is of high importance for the growth rate of P. nobilis juveniles in their natural habitat due to lower growth during winter. Anthropogenic pressure was the main obstacle for development of P. nobilis populations during the study period, while biological pollution as the main threat for P. nobilis survival will be the subject of further studies in the Montenegrin Adriatic coast.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Recruitment and Growth of the Fan Mussel Pinna nobilis in the Montenegrin Adriatic Coast and Comparison with the Western Mediterranean",
pages = "213-193",
volume = "109",
doi = "10.1007/698_2021_749"
}

Martinović, R., Petović, S., Joksimović, D., Bunet, R., Couvray, S., Kirchhofer, D., Simide, R., Garcia-March, J.R., Tena-Medialdea, J., Castelli, A., Gačić, Z., Bonnefont, J.-L.,& Vicente, N.. (2021). Recruitment and Growth of the Fan Mussel Pinna nobilis in the Montenegrin Adriatic Coast and Comparison with the Western Mediterranean. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 109, 193-213.
https://doi.org/10.1007/698_2021_749

Martinović R, Petović S, Joksimović D, Bunet R, Couvray S, Kirchhofer D, Simide R, Garcia-March J, Tena-Medialdea J, Castelli A, Gačić Z, Bonnefont J, Vicente N. Recruitment and Growth of the Fan Mussel Pinna nobilis in the Montenegrin Adriatic Coast and Comparison with the Western Mediterranean. in Handbook of Environmental Chemistry. 2021;109:193-213.
doi:10.1007/698_2021_749 .

Martinović, Rajko, Petović, S., Joksimović, D., Bunet, R., Couvray, S., Kirchhofer, D., Simide, R., Garcia-March, J.R., Tena-Medialdea, J., Castelli, A., Gačić, Zoran, Bonnefont, J.-L., Vicente, N., "Recruitment and Growth of the Fan Mussel Pinna nobilis in the Montenegrin Adriatic Coast and Comparison with the Western Mediterranean" in Handbook of Environmental Chemistry, 109 (2021):193-213,
https://doi.org/10.1007/698_2021_749 . .

TY  - JOUR
AU  - Jovanović-Maric, Jovana M.
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Smiljanic, Petar B.
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1369
AB  - This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers
EP  - 229
IS  - 2
SP  - 224
VL  - 105
DO  - 10.1007/s00128-020-02944-4
ER  - 

@article{
author = "Jovanović-Maric, Jovana M. and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Smiljanic, Petar B. and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2020",
abstract = "This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers",
pages = "229-224",
number = "2",
volume = "105",
doi = "10.1007/s00128-020-02944-4"
}

Jovanović-Maric, J. M., Kracun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Smiljanic, P. B., Gačić, Z.,& Vukovic-Gacic, B.. (2020). Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 105(2), 224-229.
https://doi.org/10.1007/s00128-020-02944-4

Jovanović-Maric JM, Kracun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Smiljanic PB, Gačić Z, Vukovic-Gacic B. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology. 2020;105(2):224-229.
doi:10.1007/s00128-020-02944-4 .

Jovanović-Maric, Jovana M., Kracun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Smiljanic, Petar B., Gačić, Zoran, Vukovic-Gacic, Branka, "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers" in Bulletin of Environmental Contamination and Toxicology, 105, no. 2 (2020):224-229,
https://doi.org/10.1007/s00128-020-02944-4 . .

TY  - JOUR
AU  - Jaćimović, Milica
AU  - Lenhardt, Mirjana
AU  - Krpo-Cetković, Jasmina
AU  - Jarić, Ivan
AU  - Gačić, Zoran
AU  - Hegediš, Aleksandar
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1242
AB  - The life-history traits and population dynamics of the black bullhead Ameiurus melas (Rafinesque) were studied monthly from August 2009 to December 2012 in Lake Sava in Belgrade (Serbia). This period included a mass mortality event in May 2011 and subsequent population recovery during 2012. Available evidence suggests the mass mortality was caused by European catfish virus that only affected the black bullhead. Changes in key life-history traits and the recovery potential (increase in catch-per-unit-effort, doubling of young-of-the-year to adult ratio, earlier maturation and increased fecundity) following the mass mortality event suggest population resilience typical of a recurring boom-bust pattern and demonstrate the species' capacity to recover rapidly and re-establish following disturbance. Repeated systematic mass removals of both juveniles and adults (using fyke nets) will be required if future fishery management measures aimed at limiting the population size are to be effective.
PB  - Wiley, Hoboken
T2  - Fisheries Management and Ecology
T1  - Boom-bust like dynamics of invasive black bullhead (Ameiurus melas) in Lake Sava (Serbia)
EP  - 164
IS  - 2
SP  - 153
VL  - 26
DO  - 10.1111/fme.12335
ER  - 

@article{
author = "Jaćimović, Milica and Lenhardt, Mirjana and Krpo-Cetković, Jasmina and Jarić, Ivan and Gačić, Zoran and Hegediš, Aleksandar",
year = "2019",
abstract = "The life-history traits and population dynamics of the black bullhead Ameiurus melas (Rafinesque) were studied monthly from August 2009 to December 2012 in Lake Sava in Belgrade (Serbia). This period included a mass mortality event in May 2011 and subsequent population recovery during 2012. Available evidence suggests the mass mortality was caused by European catfish virus that only affected the black bullhead. Changes in key life-history traits and the recovery potential (increase in catch-per-unit-effort, doubling of young-of-the-year to adult ratio, earlier maturation and increased fecundity) following the mass mortality event suggest population resilience typical of a recurring boom-bust pattern and demonstrate the species' capacity to recover rapidly and re-establish following disturbance. Repeated systematic mass removals of both juveniles and adults (using fyke nets) will be required if future fishery management measures aimed at limiting the population size are to be effective.",
publisher = "Wiley, Hoboken",
journal = "Fisheries Management and Ecology",
title = "Boom-bust like dynamics of invasive black bullhead (Ameiurus melas) in Lake Sava (Serbia)",
pages = "164-153",
number = "2",
volume = "26",
doi = "10.1111/fme.12335"
}

Jaćimović, M., Lenhardt, M., Krpo-Cetković, J., Jarić, I., Gačić, Z.,& Hegediš, A.. (2019). Boom-bust like dynamics of invasive black bullhead (Ameiurus melas) in Lake Sava (Serbia). in Fisheries Management and Ecology
Wiley, Hoboken., 26(2), 153-164.
https://doi.org/10.1111/fme.12335

Jaćimović M, Lenhardt M, Krpo-Cetković J, Jarić I, Gačić Z, Hegediš A. Boom-bust like dynamics of invasive black bullhead (Ameiurus melas) in Lake Sava (Serbia). in Fisheries Management and Ecology. 2019;26(2):153-164.
doi:10.1111/fme.12335 .

Jaćimović, Milica, Lenhardt, Mirjana, Krpo-Cetković, Jasmina, Jarić, Ivan, Gačić, Zoran, Hegediš, Aleksandar, "Boom-bust like dynamics of invasive black bullhead (Ameiurus melas) in Lake Sava (Serbia)" in Fisheries Management and Ecology, 26, no. 2 (2019):153-164,
https://doi.org/10.1111/fme.12335 . .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1736
AB  - Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
T1  - Significance of genotoxicity and toxicity evaluation of freshwater bodies
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1736
ER  - 

@conference{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić-Vuković, Jovana and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.",
title = "Significance of genotoxicity and toxicity evaluation of freshwater bodies",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1736"
}

Sunjog, K., Kolarević, S., Kracun-Kolarevic, M., Kostić-Vuković, J., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
Institute of Marine Biology, University of Montenegro., 114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736

Sunjog K, Kolarević S, Kracun-Kolarevic M, Kostić-Vuković J, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vuković-Gačić B. Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.. 2019;:114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić-Vuković, Jovana, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Significance of genotoxicity and toxicity evaluation of freshwater bodies" in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro. (2019):114,
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1723
AB  - Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Bioassays in assessment of environmental pollution
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Bioassays in assessment of environmental pollution",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1723"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Bioassays in assessment of environmental pollution" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):115,
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1278
AB  - Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)
EP  - 9243
IS  - 9
SP  - 9232
VL  - 26
DO  - 10.1007/s11356-019-04274-3
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)",
pages = "9243-9232",
number = "9",
volume = "26",
doi = "10.1007/s11356-019-04274-3"
}

Sunjog, K., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 26(9), 9232-9243.
https://doi.org/10.1007/s11356-019-04274-3

Sunjog K, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vukovic-Gacic B. Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research. 2019;26(9):9232-9243.
doi:10.1007/s11356-019-04274-3 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)" in Environmental Science and Pollution Research, 26, no. 9 (2019):9232-9243,
https://doi.org/10.1007/s11356-019-04274-3 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Mićković, Branislav
AU  - Gacic, Luka
AU  - Damjanović, Ilija
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1248
AB  - A four-parameter model of spectral sensitivity curves was developed. Empirical equations were designed for A(1)- and A(2)-based visual pigments with the main a-band maximum absorptions (lambda(max)) from 350 nm, near the ultraviolet, up to 635 nm in the far-red part of the spectrum. Subtraction of the alpha-band from the full absorbance spectrum left a "beta-band" described by a lambda(max)-dependent Gaussian equation. Compatibility of our templates with A(1)- and A(2)-based spectra was tested on the electroretinographic (ERG-derived) scotopic action spectra recorded in dogfish shark, eel, Prussian carp and perch. To more precisely estimate the accuracy of our model, we compared it with widely used templates for visual pigments. There was almost no difference between the tested models in fitting the above-mentioned spectral data. One of the advantages of our model is that in the fitting of spectral sensitivity data it uses non-transformed wavelengths and the shape of the curve remains the same for a broad range of lambda(max) values. Compared to multiparameter templates of other authors, our model was designed with fewer (four) parameters, which we believe can bring us closer to understanding the true nature of the absorption curve.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - New spectral templates for rhodopsin and porphyropsin visual pigments
EP  - 110
IS  - 1
SP  - 103
VL  - 71
DO  - 10.2298/ABS180822052G
ER  - 

@article{
author = "Gačić, Zoran and Mićković, Branislav and Gacic, Luka and Damjanović, Ilija",
year = "2019",
abstract = "A four-parameter model of spectral sensitivity curves was developed. Empirical equations were designed for A(1)- and A(2)-based visual pigments with the main a-band maximum absorptions (lambda(max)) from 350 nm, near the ultraviolet, up to 635 nm in the far-red part of the spectrum. Subtraction of the alpha-band from the full absorbance spectrum left a "beta-band" described by a lambda(max)-dependent Gaussian equation. Compatibility of our templates with A(1)- and A(2)-based spectra was tested on the electroretinographic (ERG-derived) scotopic action spectra recorded in dogfish shark, eel, Prussian carp and perch. To more precisely estimate the accuracy of our model, we compared it with widely used templates for visual pigments. There was almost no difference between the tested models in fitting the above-mentioned spectral data. One of the advantages of our model is that in the fitting of spectral sensitivity data it uses non-transformed wavelengths and the shape of the curve remains the same for a broad range of lambda(max) values. Compared to multiparameter templates of other authors, our model was designed with fewer (four) parameters, which we believe can bring us closer to understanding the true nature of the absorption curve.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "New spectral templates for rhodopsin and porphyropsin visual pigments",
pages = "110-103",
number = "1",
volume = "71",
doi = "10.2298/ABS180822052G"
}

Gačić, Z., Mićković, B., Gacic, L.,& Damjanović, I.. (2019). New spectral templates for rhodopsin and porphyropsin visual pigments. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(1), 103-110.
https://doi.org/10.2298/ABS180822052G

Gačić Z, Mićković B, Gacic L, Damjanović I. New spectral templates for rhodopsin and porphyropsin visual pigments. in Archives of Biological Sciences. 2019;71(1):103-110.
doi:10.2298/ABS180822052G .

Gačić, Zoran, Mićković, Branislav, Gacic, Luka, Damjanović, Ilija, "New spectral templates for rhodopsin and porphyropsin visual pigments" in Archives of Biological Sciences, 71, no. 1 (2019):103-110,
https://doi.org/10.2298/ABS180822052G . .

TY  - JOUR
AU  - Damjanović, Ilija
AU  - Maximov, Pavel V.
AU  - Aliper, Alexey T.
AU  - Zaichikova, Alisa A.
AU  - Gačić, Zoran
AU  - Maximova, Elena M.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1241
AB  - Responses of direction selective (DS) units of retinal and tectal origin were recorded extracellularly from the tectum opticum (TO) of immobilized fish. The data were collected from three cyprinid species - goldfish, carp and roach. Responses of the retinal DS ganglion cells (GCs) were recorded from their axon terminals in the superficial layers of TO. According to their preferred directions DS GCs, characterized by small receptive fields (3 8), can be divided in three distinct groups, each group containing ON and OFF subtypes approximately in equal quantity. Conversely, direction-selective tectal neurons (DS TNs), recorded at two different tectal levels deeper than the zone of retinal DS afferents, are characterized by large receptive fields (up to 60) and are indifferent to any sign of contrast i.e. can be considered as ON-OFF type units. Fish DS TNs unlike the retinal DS GCs, select four preferred directions. Three types of tectal DS units prefer practically the same directions as those already selected on the retinal level caudo-rostral, dorso-ventral and ventro-dorsal. The fact that three preferred directions of DS GCs and DS TNs coincide allows us to assume that three types of DS GCs are input neurons for corresponding types of DS TNs. The fourth group of DS TNs has the emergent rostro-caudal preference not explicitly present in any of the DS GC inputs. These units are recorded in deep TO layers exclusively. Receptive fields of these DS neurons could be entirely formed on the tectal level. Possible interrelations between retinal and tectal DS units are discussed.
PB  - Elsevier Science Bv, Amsterdam
T2  - Brain Research
T1  - Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish
EP  - 26
SP  - 20
VL  - 1708
DO  - 10.1016/j.brainres.2018.12.006
ER  - 

@article{
author = "Damjanović, Ilija and Maximov, Pavel V. and Aliper, Alexey T. and Zaichikova, Alisa A. and Gačić, Zoran and Maximova, Elena M.",
year = "2019",
abstract = "Responses of direction selective (DS) units of retinal and tectal origin were recorded extracellularly from the tectum opticum (TO) of immobilized fish. The data were collected from three cyprinid species - goldfish, carp and roach. Responses of the retinal DS ganglion cells (GCs) were recorded from their axon terminals in the superficial layers of TO. According to their preferred directions DS GCs, characterized by small receptive fields (3 8), can be divided in three distinct groups, each group containing ON and OFF subtypes approximately in equal quantity. Conversely, direction-selective tectal neurons (DS TNs), recorded at two different tectal levels deeper than the zone of retinal DS afferents, are characterized by large receptive fields (up to 60) and are indifferent to any sign of contrast i.e. can be considered as ON-OFF type units. Fish DS TNs unlike the retinal DS GCs, select four preferred directions. Three types of tectal DS units prefer practically the same directions as those already selected on the retinal level caudo-rostral, dorso-ventral and ventro-dorsal. The fact that three preferred directions of DS GCs and DS TNs coincide allows us to assume that three types of DS GCs are input neurons for corresponding types of DS TNs. The fourth group of DS TNs has the emergent rostro-caudal preference not explicitly present in any of the DS GC inputs. These units are recorded in deep TO layers exclusively. Receptive fields of these DS neurons could be entirely formed on the tectal level. Possible interrelations between retinal and tectal DS units are discussed.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Brain Research",
title = "Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish",
pages = "26-20",
volume = "1708",
doi = "10.1016/j.brainres.2018.12.006"
}

Damjanović, I., Maximov, P. V., Aliper, A. T., Zaichikova, A. A., Gačić, Z.,& Maximova, E. M.. (2019). Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish. in Brain Research
Elsevier Science Bv, Amsterdam., 1708, 20-26.
https://doi.org/10.1016/j.brainres.2018.12.006

Damjanović I, Maximov PV, Aliper AT, Zaichikova AA, Gačić Z, Maximova EM. Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish. in Brain Research. 2019;1708:20-26.
doi:10.1016/j.brainres.2018.12.006 .

Damjanović, Ilija, Maximov, Pavel V., Aliper, Alexey T., Zaichikova, Alisa A., Gačić, Zoran, Maximova, Elena M., "Putative targets of direction-selective retinal ganglion cells in the tectum opticum of cyprinid fish" in Brain Research, 1708 (2019):20-26,
https://doi.org/10.1016/j.brainres.2018.12.006 . .

TY  - JOUR
AU  - Aliper, Alexey T.
AU  - Zaichikova, Alisa A.
AU  - Damjanović, Ilija
AU  - Maximov, Paul V.
AU  - Kasparson, Anna A.
AU  - Gačić, Zoran
AU  - Maximova, Elena M.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1239
AB  - Single-unit responses of retinal ganglion cells (GCs) were recorded extracellularly from their axonal terminals in the tectum opticum (TO) of the intact fish (goldfish, carp). The depths of retinal units consecutively recorded along the track of the microelectrode were measured. At the depth of around 50m, the responses of six types of direction-selective (DS) GCs were regularly recorded. Responses of two types of orientation-selective (OS) GCs and detectors of white and black spots occurred approximately 50m deeper. Responses of GCs with dark- and light-sustained activity were recorded deeper than all others, at about 200m. The receptive fields of consecutively recorded units overlap, so they analyze the same fragment of the visual scene, focused by eye optic on the photoreceptor raster. The responses of pairs of DS GCs (ON and OFF units that preferred same direction of stimulus movement) and OS GCs (detectors of vertical and horizontal lines) were often simultaneously recorded at one position of the microelectrode. (The paired recordings of certain units amounted about fourth part of all recordings.) This suggests that their axonal arborizations are located close to each other in the tectal retinorecipient layer. Electrophysiological method, thus, allows to indirectly clarify and make precise the morphology of the retino-tectal connections and to establish a morpho-physiological correspondence.
PB  - Springer, Dordrecht
T2  - Fish Physiology and Biochemistry
T1  - Updated functional segregation of retinal ganglion cell projections in the tectum of a cyprinid fishfurther elaboration based on microelectrode recordings
EP  - 792
IS  - 2
SP  - 773
VL  - 45
DO  - 10.1007/s10695-018-0603-0
ER  - 

@article{
author = "Aliper, Alexey T. and Zaichikova, Alisa A. and Damjanović, Ilija and Maximov, Paul V. and Kasparson, Anna A. and Gačić, Zoran and Maximova, Elena M.",
year = "2019",
abstract = "Single-unit responses of retinal ganglion cells (GCs) were recorded extracellularly from their axonal terminals in the tectum opticum (TO) of the intact fish (goldfish, carp). The depths of retinal units consecutively recorded along the track of the microelectrode were measured. At the depth of around 50m, the responses of six types of direction-selective (DS) GCs were regularly recorded. Responses of two types of orientation-selective (OS) GCs and detectors of white and black spots occurred approximately 50m deeper. Responses of GCs with dark- and light-sustained activity were recorded deeper than all others, at about 200m. The receptive fields of consecutively recorded units overlap, so they analyze the same fragment of the visual scene, focused by eye optic on the photoreceptor raster. The responses of pairs of DS GCs (ON and OFF units that preferred same direction of stimulus movement) and OS GCs (detectors of vertical and horizontal lines) were often simultaneously recorded at one position of the microelectrode. (The paired recordings of certain units amounted about fourth part of all recordings.) This suggests that their axonal arborizations are located close to each other in the tectal retinorecipient layer. Electrophysiological method, thus, allows to indirectly clarify and make precise the morphology of the retino-tectal connections and to establish a morpho-physiological correspondence.",
publisher = "Springer, Dordrecht",
journal = "Fish Physiology and Biochemistry",
title = "Updated functional segregation of retinal ganglion cell projections in the tectum of a cyprinid fishfurther elaboration based on microelectrode recordings",
pages = "792-773",
number = "2",
volume = "45",
doi = "10.1007/s10695-018-0603-0"
}

Aliper, A. T., Zaichikova, A. A., Damjanović, I., Maximov, P. V., Kasparson, A. A., Gačić, Z.,& Maximova, E. M.. (2019). Updated functional segregation of retinal ganglion cell projections in the tectum of a cyprinid fishfurther elaboration based on microelectrode recordings. in Fish Physiology and Biochemistry
Springer, Dordrecht., 45(2), 773-792.
https://doi.org/10.1007/s10695-018-0603-0

Aliper AT, Zaichikova AA, Damjanović I, Maximov PV, Kasparson AA, Gačić Z, Maximova EM. Updated functional segregation of retinal ganglion cell projections in the tectum of a cyprinid fishfurther elaboration based on microelectrode recordings. in Fish Physiology and Biochemistry. 2019;45(2):773-792.
doi:10.1007/s10695-018-0603-0 .

Aliper, Alexey T., Zaichikova, Alisa A., Damjanović, Ilija, Maximov, Paul V., Kasparson, Anna A., Gačić, Zoran, Maximova, Elena M., "Updated functional segregation of retinal ganglion cell projections in the tectum of a cyprinid fishfurther elaboration based on microelectrode recordings" in Fish Physiology and Biochemistry, 45, no. 2 (2019):773-792,
https://doi.org/10.1007/s10695-018-0603-0 . .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Jovanovic, J.
AU  - Kolarevic, S.
AU  - Miloskovic, A.
AU  - Radojkovic, N.
AU  - Simic, V.
AU  - Dojcinovic, B.
AU  - Kracun-Kolarevic, M.
AU  - Paunovic, M
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, J
AU  - Gačić, Zoran
AU  - Zegura, B
AU  - Vukovic Gacic, B
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2152
AB  - Human activity has the great negative impact on water quality. As a consequence, deterioration of freshwater ecosystems has the effect on whole wildlife. Some of the pollutants which are realised into water could interact with DNA molecule and lead to DNA damage. The Velika Morava River is one of the larger tributaries of the Danube River. The major ecological problem of the Velika Morava River basin is discharging of municipal and industrial wastewaters. The aim of our study was the evaluation of genotoxic potential of the Velika Morava River basin. For this purpose, we employed battery of in vitro and in situ bioassays. From 9 selected sites the water samples and blood samples of Alburnus alburnus were collected. Within in vitro approach, the genotoxicity of water samples was tested using the SOS/umuC test on Salmonella thyphimurium TA1535/pSK1002 and alkaline comet assay on the HepG2 cell line. On the other hand, in situ approach included the alkaline and Fpg modified comet assay and the micronucleus test on bleak (A. alburnus) erythrocytes. The obtained results indicated differences of in vitro and in situ tests to evaluate genotoxic potential. The effects of genotoxicity were evident only in tests performed in situ, which is not surprising considering constant exposure of collected organisms to environmental stress. Differential sensitivity was found also within in situ tests where the alkaline comet assay showed the highest potential in distinguishing between sites. So, in accordance with results, we could conclude that application of the battery of in vitro and in situ bioassays is appropriate for assessment of genotoxic potential.
PB  - University of Agriculture in Krakow
PB  - Louisiana State University
C3  - Central and Eastern European Conference on Health and the Environment Krakow 2018
T1  - APPLICATION OF IN VITRO AND IN SITU BIOASSAYS FOR EVALUATION OF WATER GENOTOXIC POTENTIAL
EP  - 59
SP  - 59
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2152
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Jovanovic, J. and Kolarevic, S. and Miloskovic, A. and Radojkovic, N. and Simic, V. and Dojcinovic, B. and Kracun-Kolarevic, M. and Paunovic, M and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, J and Gačić, Zoran and Zegura, B and Vukovic Gacic, B",
year = "2018",
abstract = "Human activity has the great negative impact on water quality. As a consequence, deterioration of freshwater ecosystems has the effect on whole wildlife. Some of the pollutants which are realised into water could interact with DNA molecule and lead to DNA damage. The Velika Morava River is one of the larger tributaries of the Danube River. The major ecological problem of the Velika Morava River basin is discharging of municipal and industrial wastewaters. The aim of our study was the evaluation of genotoxic potential of the Velika Morava River basin. For this purpose, we employed battery of in vitro and in situ bioassays. From 9 selected sites the water samples and blood samples of Alburnus alburnus were collected. Within in vitro approach, the genotoxicity of water samples was tested using the SOS/umuC test on Salmonella thyphimurium TA1535/pSK1002 and alkaline comet assay on the HepG2 cell line. On the other hand, in situ approach included the alkaline and Fpg modified comet assay and the micronucleus test on bleak (A. alburnus) erythrocytes. The obtained results indicated differences of in vitro and in situ tests to evaluate genotoxic potential. The effects of genotoxicity were evident only in tests performed in situ, which is not surprising considering constant exposure of collected organisms to environmental stress. Differential sensitivity was found also within in situ tests where the alkaline comet assay showed the highest potential in distinguishing between sites. So, in accordance with results, we could conclude that application of the battery of in vitro and in situ bioassays is appropriate for assessment of genotoxic potential.",
publisher = "University of Agriculture in Krakow, Louisiana State University",
journal = "Central and Eastern European Conference on Health and the Environment Krakow 2018",
title = "APPLICATION OF IN VITRO AND IN SITU BIOASSAYS FOR EVALUATION OF WATER GENOTOXIC POTENTIAL",
pages = "59-59",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2152"
}

Đorđević Aleksić, J., Jovanovic, J., Kolarevic, S., Miloskovic, A., Radojkovic, N., Simic, V., Dojcinovic, B., Kracun-Kolarevic, M., Paunovic, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Gačić, Z., Zegura, B.,& Vukovic Gacic, B.. (2018). APPLICATION OF IN VITRO AND IN SITU BIOASSAYS FOR EVALUATION OF WATER GENOTOXIC POTENTIAL. in Central and Eastern European Conference on Health and the Environment Krakow 2018
University of Agriculture in Krakow., 59-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2152

Đorđević Aleksić J, Jovanovic J, Kolarevic S, Miloskovic A, Radojkovic N, Simic V, Dojcinovic B, Kracun-Kolarevic M, Paunovic M, Kostić-Vuković J, Sunjog K, Timilijic J, Gačić Z, Zegura B, Vukovic Gacic B. APPLICATION OF IN VITRO AND IN SITU BIOASSAYS FOR EVALUATION OF WATER GENOTOXIC POTENTIAL. in Central and Eastern European Conference on Health and the Environment Krakow 2018. 2018;:59-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2152 .

Đorđević Aleksić, Jelena, Jovanovic, J., Kolarevic, S., Miloskovic, A., Radojkovic, N., Simic, V., Dojcinovic, B., Kracun-Kolarevic, M., Paunovic, M, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, J, Gačić, Zoran, Zegura, B, Vukovic Gacic, B, "APPLICATION OF IN VITRO AND IN SITU BIOASSAYS FOR EVALUATION OF WATER GENOTOXIC POTENTIAL" in Central and Eastern European Conference on Health and the Environment Krakow 2018 (2018):59-59,
https://hdl.handle.net/21.15107/rcub_rimsi_2152 .

TY  - CONF
AU  - Hegediš, Aleksandar
AU  - Simonović, Predrag
AU  - Smederevac-Lalić, Marija
AU  - Skorić, Stefan
AU  - Višnjić Jeftić, Željka
AU  - Jaćimović, Milica
AU  - Jovičić, Katarina
AU  - Lenhardt, Mirjana
AU  - Mićković, Branislav
AU  - Nikčević, Miroslav
AU  - Gačić, Zoran
AU  - Nikolic, Vera
AU  - Tošić, A
AU  - Škraba-Jurlina, D
AU  - Kanjuh, T
AU  - Regner, Slobodan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1686
AB  - Ovaj rad ima za cilj da ustanovi koja je uloga zakona u dobrom upravljanju ribolovnim 
resursima. Zaštita i održivo korišćenje ribljeg fonda u Srbiji regulisano je istoimenim 
zakonom, a odvija se na 17 ribarskih područja i 29 ribarskih područja u zaštićenim prirodnim 
dobrima. Na 17 osnovnih ribarskih područja upravljaju „javna preduzeća“ ili privatne firme 
u statusu „doo“. Kod većih područja, posebno u Vojvodini, postoji više upravljača za jedno 
ribarsko područje. U zaštićenim prirodnim dobrima upravljači su ujedno i korisnici ribljeg 
fonda. U periodu od 2006. do 2017. godine Zakon o zaštiti i održivom korišćenju ribljeg 
fonda menjan je u dva navrata: 2009. i 2014. godine. Podzakonska akta su takođe podložna 
vrlo čestim promenama. U radu se prate efekti promene zakonske regulative na broj rekre ativnih i profesionalnih ribolovaca, broj ribočuvara i trendove u ulovima. Osnovni trendovi 
su da broj rekreativnih ribolovaca u navedenom periodu opada, broj profesionalnih riba ra stagnira, dok broj ribočuvara raste. Ulov ima tendenciju opadanja za sve vrste koje se 
statististički prate (šaran, som, smuđ i deverika od autohtonih vrsta, i babuška i tolstolobik 
od alohtonih). Odnos ulova u rekreativnom i komercijalnom ribolovu pokazuje značajne 
promene u poslednjih desetak godina: osim za tolstolobika, kod koga dominira komer cijalni ulov, kod ostalih vrsta je došlo do zamene u smislu da rekreativni ulov preuzima 
dominaciju u poslednjih nekoliko godina
PB  - Poljoprivredni fakultet, Univerzitet u Beogradu
C3  - 8th  International Conference "Water & Fish"
T1  - Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1686
ER  - 

@conference{
author = "Hegediš, Aleksandar and Simonović, Predrag and Smederevac-Lalić, Marija and Skorić, Stefan and Višnjić Jeftić, Željka and Jaćimović, Milica and Jovičić, Katarina and Lenhardt, Mirjana and Mićković, Branislav and Nikčević, Miroslav and Gačić, Zoran and Nikolic, Vera and Tošić, A and Škraba-Jurlina, D and Kanjuh, T and Regner, Slobodan",
year = "2018",
abstract = "Ovaj rad ima za cilj da ustanovi koja je uloga zakona u dobrom upravljanju ribolovnim 
resursima. Zaštita i održivo korišćenje ribljeg fonda u Srbiji regulisano je istoimenim 
zakonom, a odvija se na 17 ribarskih područja i 29 ribarskih područja u zaštićenim prirodnim 
dobrima. Na 17 osnovnih ribarskih područja upravljaju „javna preduzeća“ ili privatne firme 
u statusu „doo“. Kod većih područja, posebno u Vojvodini, postoji više upravljača za jedno 
ribarsko područje. U zaštićenim prirodnim dobrima upravljači su ujedno i korisnici ribljeg 
fonda. U periodu od 2006. do 2017. godine Zakon o zaštiti i održivom korišćenju ribljeg 
fonda menjan je u dva navrata: 2009. i 2014. godine. Podzakonska akta su takođe podložna 
vrlo čestim promenama. U radu se prate efekti promene zakonske regulative na broj rekre ativnih i profesionalnih ribolovaca, broj ribočuvara i trendove u ulovima. Osnovni trendovi 
su da broj rekreativnih ribolovaca u navedenom periodu opada, broj profesionalnih riba ra stagnira, dok broj ribočuvara raste. Ulov ima tendenciju opadanja za sve vrste koje se 
statististički prate (šaran, som, smuđ i deverika od autohtonih vrsta, i babuška i tolstolobik 
od alohtonih). Odnos ulova u rekreativnom i komercijalnom ribolovu pokazuje značajne 
promene u poslednjih desetak godina: osim za tolstolobika, kod koga dominira komer cijalni ulov, kod ostalih vrsta je došlo do zamene u smislu da rekreativni ulov preuzima 
dominaciju u poslednjih nekoliko godina",
publisher = "Poljoprivredni fakultet, Univerzitet u Beogradu",
journal = "8th  International Conference "Water & Fish"",
title = "Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1686"
}

Hegediš, A., Simonović, P., Smederevac-Lalić, M., Skorić, S., Višnjić Jeftić, Ž., Jaćimović, M., Jovičić, K., Lenhardt, M., Mićković, B., Nikčević, M., Gačić, Z., Nikolic, V., Tošić, A., Škraba-Jurlina, D., Kanjuh, T.,& Regner, S.. (2018). Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017. in 8th  International Conference "Water & Fish"
Poljoprivredni fakultet, Univerzitet u Beogradu..
https://hdl.handle.net/21.15107/rcub_rimsi_1686

Hegediš A, Simonović P, Smederevac-Lalić M, Skorić S, Višnjić Jeftić Ž, Jaćimović M, Jovičić K, Lenhardt M, Mićković B, Nikčević M, Gačić Z, Nikolic V, Tošić A, Škraba-Jurlina D, Kanjuh T, Regner S. Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017. in 8th  International Conference "Water & Fish". 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_1686 .

Hegediš, Aleksandar, Simonović, Predrag, Smederevac-Lalić, Marija, Skorić, Stefan, Višnjić Jeftić, Željka, Jaćimović, Milica, Jovičić, Katarina, Lenhardt, Mirjana, Mićković, Branislav, Nikčević, Miroslav, Gačić, Zoran, Nikolic, Vera, Tošić, A, Škraba-Jurlina, D, Kanjuh, T, Regner, Slobodan, "Different aspect of sustainable use of fish resources in Serbia for the period 2006-2017" in 8th  International Conference "Water & Fish" (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_1686 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1667
AB  - Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.
PB  - Institute of Geography of SAS
C3  - The 42nd IAD Conference 2018, Smolenice, Slovakia
T1  - DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1667
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.",
publisher = "Institute of Geography of SAS",
journal = "The 42nd IAD Conference 2018, Smolenice, Slovakia",
title = "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1667"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia
Institute of Geography of SAS., 34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia. 2018;:34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution" in The 42nd IAD Conference 2018, Smolenice, Slovakia (2018):34-34,
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1681
AB  - The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)
EP  - 162
SP  - 162
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1681
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)",
pages = "162-162",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1681"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):162-162,
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

TY  - JOUR
AU  - Andjus, Stefan
AU  - Nikolic, Nadja
AU  - Dobricic, Valerija
AU  - Marjanović, An
AU  - Gačić, Zoran
AU  - Branković, Goran
AU  - Aković, Maja R.
AU  - Paunović, Momir
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1134
AB  - Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.
PB  - Pagepress Publ, Pavia
T2  - Journal of Limnology
T1  - Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study
EP  - 208
IS  - 2
SP  - 199
VL  - 77
DO  - 10.4081/jlimnol.2017.1677
ER  - 

@article{
author = "Andjus, Stefan and Nikolic, Nadja and Dobricic, Valerija and Marjanović, An and Gačić, Zoran and Branković, Goran and Aković, Maja R. and Paunović, Momir",
year = "2018",
abstract = "Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.",
publisher = "Pagepress Publ, Pavia",
journal = "Journal of Limnology",
title = "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study",
pages = "208-199",
number = "2",
volume = "77",
doi = "10.4081/jlimnol.2017.1677"
}

Andjus, S., Nikolic, N., Dobricic, V., Marjanović, A., Gačić, Z., Branković, G., Aković, M. R.,& Paunović, M.. (2018). Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology
Pagepress Publ, Pavia., 77(2), 199-208.
https://doi.org/10.4081/jlimnol.2017.1677

Andjus S, Nikolic N, Dobricic V, Marjanović A, Gačić Z, Branković G, Aković MR, Paunović M. Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology. 2018;77(2):199-208.
doi:10.4081/jlimnol.2017.1677 .

Andjus, Stefan, Nikolic, Nadja, Dobricic, Valerija, Marjanović, An, Gačić, Zoran, Branković, Goran, Aković, Maja R., Paunović, Momir, "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study" in Journal of Limnology, 77, no. 2 (2018):199-208,
https://doi.org/10.4081/jlimnol.2017.1677 . .

TY  - JOUR
AU  - Smederevac-Lalić, Marija
AU  - Kalauzi, Aleksandar
AU  - Regner, Slobodan
AU  - Navodaru, Ion
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1148
AB  - The relationship between the Lower Danube River level and Romanian annual catches of Pontic shad (Alosa immaculata, Bennett 1835) were analyzed. For analysis of long term data on the Danube River water level and Pontic shad catch, combinations of different methods were applied using statistical programs, SPSS 13.0 and MATLAB 6. Periodograms, containing cyclic patterns, were obtained using Fourier analysis. Significant oscillations were determined with Fisher-Whittle' s tests and residuals were calculated after subtracting these significant oscillations from the original signals. Autoregressive moving average (ARMA) models of residuals were finally applied. Results indicated that river water levels, and especially those in May, greatly explained the fluctuations of Pontic shad catch. Annual landings varied greatly and appeared to be cyclic. Varying river flow was considered to be one of the most important factors that cause fluctuations in the size of populations. Forecast indicates gradual increase of the catch in the next decade, followed by a decrease in other decades. Estimated as a vulnerable species of fish by the IUCN, development of the forecasting model of the future catch oscillations could be very helpful to regulate fishing efforts towards the sustainable use of stocks and species conservation.
PB  - Iranian Fisheries Science Research Inst-Ifsri, Tehran
T2  - Iranian Journal of Fisheries Sciences
T1  - Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River
EP  - 457
IS  - 3
SP  - 443
VL  - 17
DO  - 10.22092/IJFS.2018.116611
ER  - 

@article{
author = "Smederevac-Lalić, Marija and Kalauzi, Aleksandar and Regner, Slobodan and Navodaru, Ion and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana",
year = "2018",
abstract = "The relationship between the Lower Danube River level and Romanian annual catches of Pontic shad (Alosa immaculata, Bennett 1835) were analyzed. For analysis of long term data on the Danube River water level and Pontic shad catch, combinations of different methods were applied using statistical programs, SPSS 13.0 and MATLAB 6. Periodograms, containing cyclic patterns, were obtained using Fourier analysis. Significant oscillations were determined with Fisher-Whittle' s tests and residuals were calculated after subtracting these significant oscillations from the original signals. Autoregressive moving average (ARMA) models of residuals were finally applied. Results indicated that river water levels, and especially those in May, greatly explained the fluctuations of Pontic shad catch. Annual landings varied greatly and appeared to be cyclic. Varying river flow was considered to be one of the most important factors that cause fluctuations in the size of populations. Forecast indicates gradual increase of the catch in the next decade, followed by a decrease in other decades. Estimated as a vulnerable species of fish by the IUCN, development of the forecasting model of the future catch oscillations could be very helpful to regulate fishing efforts towards the sustainable use of stocks and species conservation.",
publisher = "Iranian Fisheries Science Research Inst-Ifsri, Tehran",
journal = "Iranian Journal of Fisheries Sciences",
title = "Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River",
pages = "457-443",
number = "3",
volume = "17",
doi = "10.22092/IJFS.2018.116611"
}

Smederevac-Lalić, M., Kalauzi, A., Regner, S., Navodaru, I., Višnjić Jeftić, Ž., Gačić, Z.,& Lenhardt, M.. (2018). Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River. in Iranian Journal of Fisheries Sciences
Iranian Fisheries Science Research Inst-Ifsri, Tehran., 17(3), 443-457.
https://doi.org/10.22092/IJFS.2018.116611

Smederevac-Lalić M, Kalauzi A, Regner S, Navodaru I, Višnjić Jeftić Ž, Gačić Z, Lenhardt M. Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River. in Iranian Journal of Fisheries Sciences. 2018;17(3):443-457.
doi:10.22092/IJFS.2018.116611 .

Smederevac-Lalić, Marija, Kalauzi, Aleksandar, Regner, Slobodan, Navodaru, Ion, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, "Analysis and forecast of Pontic shad (Alosa immaculata) catch in the Danube River" in Iranian Journal of Fisheries Sciences, 17, no. 3 (2018):443-457,
https://doi.org/10.22092/IJFS.2018.116611 . .

TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milosković, Aleksandra
AU  - Radojković, Nataša
AU  - Simic, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1163
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
EP  - 1299
SP  - 1289
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milosković, Aleksandra and Radojković, Nataša and Simic, Vladica and Dojčinović, Biljana and Kracun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
pages = "1299-1289",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanović, J., Kolarević, S., Milosković, A., Radojković, N., Simic, V., Dojčinović, B., Kracun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Đorđević Aleksić, J., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milosković A, Radojković N, Simic V, Dojčinović B, Kracun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijic J, Đorđević Aleksić J, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milosković, Aleksandra, Radojković, Nataša, Simic, Vladica, Dojčinović, Biljana, Kracun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1720
AB  - Seeking for a better quality of life, consumption of pharmaceuticals is constantly increasing. Pharmaceuticals are designed to be more potent, degradation resistant, to affect protein targets at relatively low doses. Many of them are not selective and became threat to non-target organisms, especially to one living in aquatic ecosystems. Because of the inability of wastewater treatment plants to eliminate these compounds in the end they reach aquatic environments through sewage system.
Aquatic invertebrates spend at least a part of their life cycle in the aquatic environment. Mobility is not one of the traits that characterize many species of aquatic invertebrates, especially freshwater mussels and aquatic worms which are almost sedentary organisms. Due to their way of life, this species are under the influence of variety of pollutants via sediment and via water column.
Genotoxic effects of cytostatics with different mode of action – alkylation agent (cisplatin - CP), antimetabolite agent (5-fluorouracil – 5-FU), plant alkaloids (etoposide - ETO, vincristine sulphate - VIN) and other neoplastic agent (imatinib mesylate - IM) were studied in vivo and in vitro on haemocytes of two freshwater mussels species Unio sp. (U. pictorum/U. tumidus), and in vivo on haemocytes and coelomocytes of tubificid species Limnodrilus udekemianus.
Experiments were organized as short-term treatments (72h for mussels/96h for worms) in static system. Level of DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay). Based on our results ranking of cytostatics by their effects on mussels was VIN>5-FU>ETO>CP>IM and on worms was 5-FU>ET. Worms have shown higher sensitivity for the negative effects of 5-FU and ET on the integrity of DNA molecule comparing with mussels. The lowest observed effect concentration (LOEC) for 5-FU was 52 μg/L for mussels and 0.52μg/L for worms. In the case of ET LOEC was 24 mg/L for mussels and 0.024 mg/L for worms. For VIN was detected difference in the response in U. pictorum and U. tumidus – LOEC for U. pictorum was 3.7 μg/L, while for U. tumidus was 36.9 μg/L. Significant damage of DNA wasn’t detected for CP and IM. Although the PEC values for tested cytostatics are lower than the ones used in our study, it must be emphasized that in the environment, organisms are under constant influence of these pollutants and organisms are struggling with the effects of mixture of pharmaceuticals and mixture of different pollutants. Impacts of these mixtures on the aquatic organisms are still unknown, and therefore, further research should consider this fact and the studies should be organized in this direction.
Acknowledgements: EU Seventh Framework Programme – Cytothreat (no. 265264). Ministry of Education, Science and Technological Development of the Republic of Serbia project no. 037009.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1720
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Kolarević, Stoimir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "Seeking for a better quality of life, consumption of pharmaceuticals is constantly increasing. Pharmaceuticals are designed to be more potent, degradation resistant, to affect protein targets at relatively low doses. Many of them are not selective and became threat to non-target organisms, especially to one living in aquatic ecosystems. Because of the inability of wastewater treatment plants to eliminate these compounds in the end they reach aquatic environments through sewage system.
Aquatic invertebrates spend at least a part of their life cycle in the aquatic environment. Mobility is not one of the traits that characterize many species of aquatic invertebrates, especially freshwater mussels and aquatic worms which are almost sedentary organisms. Due to their way of life, this species are under the influence of variety of pollutants via sediment and via water column.
Genotoxic effects of cytostatics with different mode of action – alkylation agent (cisplatin - CP), antimetabolite agent (5-fluorouracil – 5-FU), plant alkaloids (etoposide - ETO, vincristine sulphate - VIN) and other neoplastic agent (imatinib mesylate - IM) were studied in vivo and in vitro on haemocytes of two freshwater mussels species Unio sp. (U. pictorum/U. tumidus), and in vivo on haemocytes and coelomocytes of tubificid species Limnodrilus udekemianus.
Experiments were organized as short-term treatments (72h for mussels/96h for worms) in static system. Level of DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay). Based on our results ranking of cytostatics by their effects on mussels was VIN>5-FU>ETO>CP>IM and on worms was 5-FU>ET. Worms have shown higher sensitivity for the negative effects of 5-FU and ET on the integrity of DNA molecule comparing with mussels. The lowest observed effect concentration (LOEC) for 5-FU was 52 μg/L for mussels and 0.52μg/L for worms. In the case of ET LOEC was 24 mg/L for mussels and 0.024 mg/L for worms. For VIN was detected difference in the response in U. pictorum and U. tumidus – LOEC for U. pictorum was 3.7 μg/L, while for U. tumidus was 36.9 μg/L. Significant damage of DNA wasn’t detected for CP and IM. Although the PEC values for tested cytostatics are lower than the ones used in our study, it must be emphasized that in the environment, organisms are under constant influence of these pollutants and organisms are struggling with the effects of mixture of pharmaceuticals and mixture of different pollutants. Impacts of these mixtures on the aquatic organisms are still unknown, and therefore, further research should consider this fact and the studies should be organized in this direction.
Acknowledgements: EU Seventh Framework Programme – Cytothreat (no. 265264). Ministry of Education, Science and Technological Development of the Republic of Serbia project no. 037009.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1720"
}

Kračun-Kolarević, M., Kolarević, S., Kostić-Vuković, J., Sunjog, K., Gačić, Z., Paunović, M.,& Vuković-Gačić, B.. (2018). Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 135.
https://hdl.handle.net/21.15107/rcub_rimsi_1720

Kračun-Kolarević M, Kolarević S, Kostić-Vuković J, Sunjog K, Gačić Z, Paunović M, Vuković-Gačić B. Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:135.
https://hdl.handle.net/21.15107/rcub_rimsi_1720 .

Kračun-Kolarević, Margareta, Kolarević, Stoimir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Paunović, Momir, Vuković-Gačić, Branka, "Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):135,
https://hdl.handle.net/21.15107/rcub_rimsi_1720 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1160
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .
PB  - Springer International Publishing Ag, Cham
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
EP  - 712
IS  - 5
SP  - 703
VL  - 12
DO  - 10.1007/s41742-018-0127-6
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simic, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .",
publisher = "Springer International Publishing Ag, Cham",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
pages = "712-703",
number = "5",
volume = "12",
doi = "10.1007/s41742-018-0127-6"
}

Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simic, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vukovic-Gacic, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research
Springer International Publishing Ag, Cham., 12(5), 703-712.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kracun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simic V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vukovic-Gacic B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simic, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vukovic-Gacic, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712,
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kirschner, Alexander K. T.
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Gačić, Zoran
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1719
AB  - The level of genotoxic pollution was assessed along the Danube River and its most significant tributary Sava River by measuring the level of DNA damage in aquatic organisms collected from the selected sites. The Danube River survey was conducted within the Joint Danube Survey 3 project in 2013 on 34 sites along the 2285 rkm using mussels (Unio sp.) and fish (Alburnus alburnus) as bioindicators. The Sava River survey was conducted within the Globaqua project in 2015 at 12 sites along 900 rkm using fish (A. alburnus/Alburnoides bipunctatus) as bioindicators. The level of DNA damage was evaluated by the comet assay in haemocytes of mussels and blood cells of fish. The level of faecal pollution along the rivers was studied in parallel with genotoxicological surveys by using the standard indicators, total coliforms, Escherichia coli and Enterococci. Quantification was performed with Colilert/Enterolert Quanti-Tray 2000 and MPN approach. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes faecal markers have been used for tracking the source of pollution (microbial source tracking -MST). The human-associated BacHum and HF183II, the ruminantassociated BacR and the pig-associated Pig2Bac fecal markers were selected. The hotspots of faecal pollution were detected at both rivers. Presence of pollution was especially evident in the countries in which the legislation related to wastewater treatment and management is not fully implemented. In the case of the Danube River the most critical section of the river was the Pannonian plain (sector VI) while in the case of the Sava River the most affected section was the lower stretch of the river. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II in the majority of the analyzed samples. High correlation was observed between the standard fecal indicators and human associated faecal markers. Within the Danube survey, the highest levels of DNA damage were recorded in organisms from the section
VI, which is under the impact of untreated wastewater discharges. In 2013 the Sava River was characterized with a lower level of both faecal and genotoxic pollution in comparison with the Danube. Similar observations were found within the Sava River survey in 2015 where the level of DNA damage in fish specimens from Sava was lower in comparison with the samples from the Danube. At both rivers detected genotoxic potential was traceable to the deterioration of quality by communal and industrial wastewaters. Acknowledgements: International Commission for the Protection of the Danube River, EU Seventh Framework Program GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1719
ER  - 

@conference{
author = "Kolarević, Stoimir and Kirschner, Alexander K. T. and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Gačić, Zoran and Farnleitner, Andreas and Reischer, Georg and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "The level of genotoxic pollution was assessed along the Danube River and its most significant tributary Sava River by measuring the level of DNA damage in aquatic organisms collected from the selected sites. The Danube River survey was conducted within the Joint Danube Survey 3 project in 2013 on 34 sites along the 2285 rkm using mussels (Unio sp.) and fish (Alburnus alburnus) as bioindicators. The Sava River survey was conducted within the Globaqua project in 2015 at 12 sites along 900 rkm using fish (A. alburnus/Alburnoides bipunctatus) as bioindicators. The level of DNA damage was evaluated by the comet assay in haemocytes of mussels and blood cells of fish. The level of faecal pollution along the rivers was studied in parallel with genotoxicological surveys by using the standard indicators, total coliforms, Escherichia coli and Enterococci. Quantification was performed with Colilert/Enterolert Quanti-Tray 2000 and MPN approach. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes faecal markers have been used for tracking the source of pollution (microbial source tracking -MST). The human-associated BacHum and HF183II, the ruminantassociated BacR and the pig-associated Pig2Bac fecal markers were selected. The hotspots of faecal pollution were detected at both rivers. Presence of pollution was especially evident in the countries in which the legislation related to wastewater treatment and management is not fully implemented. In the case of the Danube River the most critical section of the river was the Pannonian plain (sector VI) while in the case of the Sava River the most affected section was the lower stretch of the river. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II in the majority of the analyzed samples. High correlation was observed between the standard fecal indicators and human associated faecal markers. Within the Danube survey, the highest levels of DNA damage were recorded in organisms from the section
VI, which is under the impact of untreated wastewater discharges. In 2013 the Sava River was characterized with a lower level of both faecal and genotoxic pollution in comparison with the Danube. Similar observations were found within the Sava River survey in 2015 where the level of DNA damage in fish specimens from Sava was lower in comparison with the samples from the Danube. At both rivers detected genotoxic potential was traceable to the deterioration of quality by communal and industrial wastewaters. Acknowledgements: International Commission for the Protection of the Danube River, EU Seventh Framework Program GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1719"
}

Kolarević, S., Kirschner, A. K. T., Kracun-Kolarević, M., Kostić-Vuković, J., Gačić, Z., Farnleitner, A., Reischer, G., Paunović, M.,& Vuković-Gačić, B.. (2018). Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_1719

Kolarević S, Kirschner AKT, Kracun-Kolarević M, Kostić-Vuković J, Gačić Z, Farnleitner A, Reischer G, Paunović M, Vuković-Gačić B. Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_1719 .

Kolarević, Stoimir, Kirschner, Alexander K. T., Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Gačić, Zoran, Farnleitner, Andreas, Reischer, Georg, Paunović, Momir, Vuković-Gačić, Branka, "Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):51,
https://hdl.handle.net/21.15107/rcub_rimsi_1719 .