TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1667
AB  - Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.
PB  - Institute of Geography of SAS
C3  - The 42nd IAD Conference 2018, Smolenice, Slovakia
T1  - DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1667
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.",
publisher = "Institute of Geography of SAS",
journal = "The 42nd IAD Conference 2018, Smolenice, Slovakia",
title = "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1667"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia
Institute of Geography of SAS., 34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia. 2018;:34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution" in The 42nd IAD Conference 2018, Smolenice, Slovakia (2018):34-34,
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .