TY  - JOUR
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Joksimović, Danijela
AU  - Martinović, Rajko
AU  - Ramšak, Andreja
AU  - Bajt, Oliver
AU  - Đorđević Aleksić, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2160
AB  - In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)
EP  - 12
IS  - 38
SP  - 3
VL  - 1
DO  - 10.1093/mutage/geac017
ER  - 

@article{
author = "Vuković-Gačić, Branka and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Joksimović, Danijela and Martinović, Rajko and Ramšak, Andreja and Bajt, Oliver and Đorđević Aleksić, Jelena",
year = "2023",
abstract = "In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)",
pages = "12-3",
number = "38",
volume = "1",
doi = "10.1093/mutage/geac017"
}

Vuković-Gačić, B., Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Joksimović, D., Martinović, R., Ramšak, A., Bajt, O.,& Đorđević Aleksić, J.. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis
Oxford University Press., 1(38), 3-12.
https://doi.org/10.1093/mutage/geac017

Vuković-Gačić B, Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Joksimović D, Martinović R, Ramšak A, Bajt O, Đorđević Aleksić J. Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis. 2023;1(38):3-12.
doi:10.1093/mutage/geac017 .

Vuković-Gačić, Branka, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Joksimović, Danijela, Martinović, Rajko, Ramšak, Andreja, Bajt, Oliver, Đorđević Aleksić, Jelena, "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)" in Mutagenesis, 1, no. 38 (2023):3-12,
https://doi.org/10.1093/mutage/geac017 . .

TY  - CONF
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Perošević-Bajčeta, Ana
AU  - Joksimović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5726
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2172
AB  - More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.
PB  - Institute of Oceanography and Fisheries
C3  - 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
T1  - Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2172
ER  - 

@conference{
author = "Martinović, Rajko and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Gačić, Zoran and Vuković-Gačić, Branka and Perošević-Bajčeta, Ana and Joksimović, Danijela",
year = "2022",
abstract = "More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.",
publisher = "Institute of Oceanography and Fisheries",
journal = "2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries",
title = "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2172"
}

Martinović, R., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Gačić, Z., Vuković-Gačić, B., Perošević-Bajčeta, A.,& Joksimović, D.. (2022). Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
Institute of Oceanography and Fisheries., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172

Martinović R, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Gačić Z, Vuković-Gačić B, Perošević-Bajčeta A, Joksimović D. Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries. 2022;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

Martinović, Rajko, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Gačić, Zoran, Vuković-Gačić, Branka, Perošević-Bajčeta, Ana, Joksimović, Danijela, "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast" in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries (2022):33,
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

TY  - JOUR
AU  - Martinović, Rajko
AU  - Joksimović, Danijela
AU  - García-March, José Rafael
AU  - Vicente, Nardo
AU  - Gačić, Zoran
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1573
AB  - A non-invasive laser fiber-optic method based on infrared sensors for heart rate (Hr) recording was applied to assess the physiological condition of Pinna nobilis. During 2017, the specimens of P. nobilis were sampled at three sites within the Boka Kotorska Bay, Montenegro and used for ex situ experiments with short-term reduction/restoration of ambient salinity to evaluate their physiological adaptive capacity based on heart rate recovery time (Trec). Mean Trec for specimens from Sv. Nedjelja (reference site), Dobrota and Sv. Stasije were 72 ± 3, 91 ± 7 and 117 ± 15 min, while the coefficients of variation (CV) were 0.12, 0.13 and 0.17, respectively. Resting heart rate (Hrrest) and Trec showed statistically significant differences between the groups of mussels from Dobrota and Sv. Stasije in comparison to the reference site. Statistically significant correlations were observed between Trec and shell length/width, which was not the case in comparison between Hrrest and shell length/width. The lower adaptive capacity within the P. nobilis specimens from Dobrota and Sv. Stasije in comparison to the reference site could occur due to stress induced by deterioration of environmental conditions, which could have led to impairment of the physiological state of the mussels evaluated by Hr. All the specimens of P. nobilis survived the experimental treatments; afterwards, they were successfully transplanted at the Dobrota site. The experimental unit with sensor technology applied in this study can provide Hr recording in real time and could have an application in monitoring the physiological/health state of P. nobilis individuals maintained in aquaria.
PB  - MDPI
T2  - Micromachines
T1  - Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test
IS  - 9
SP  - 1549
VL  - 13
DO  - 10.3390/mi13091549
ER  - 

@article{
author = "Martinović, Rajko and Joksimović, Danijela and García-March, José Rafael and Vicente, Nardo and Gačić, Zoran",
year = "2022",
abstract = "A non-invasive laser fiber-optic method based on infrared sensors for heart rate (Hr) recording was applied to assess the physiological condition of Pinna nobilis. During 2017, the specimens of P. nobilis were sampled at three sites within the Boka Kotorska Bay, Montenegro and used for ex situ experiments with short-term reduction/restoration of ambient salinity to evaluate their physiological adaptive capacity based on heart rate recovery time (Trec). Mean Trec for specimens from Sv. Nedjelja (reference site), Dobrota and Sv. Stasije were 72 ± 3, 91 ± 7 and 117 ± 15 min, while the coefficients of variation (CV) were 0.12, 0.13 and 0.17, respectively. Resting heart rate (Hrrest) and Trec showed statistically significant differences between the groups of mussels from Dobrota and Sv. Stasije in comparison to the reference site. Statistically significant correlations were observed between Trec and shell length/width, which was not the case in comparison between Hrrest and shell length/width. The lower adaptive capacity within the P. nobilis specimens from Dobrota and Sv. Stasije in comparison to the reference site could occur due to stress induced by deterioration of environmental conditions, which could have led to impairment of the physiological state of the mussels evaluated by Hr. All the specimens of P. nobilis survived the experimental treatments; afterwards, they were successfully transplanted at the Dobrota site. The experimental unit with sensor technology applied in this study can provide Hr recording in real time and could have an application in monitoring the physiological/health state of P. nobilis individuals maintained in aquaria.",
publisher = "MDPI",
journal = "Micromachines",
title = "Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test",
number = "9",
pages = "1549",
volume = "13",
doi = "10.3390/mi13091549"
}

Martinović, R., Joksimović, D., García-March, J. R., Vicente, N.,& Gačić, Z.. (2022). Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test. in Micromachines
MDPI., 13(9), 1549.
https://doi.org/10.3390/mi13091549

Martinović R, Joksimović D, García-March JR, Vicente N, Gačić Z. Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test. in Micromachines. 2022;13(9):1549.
doi:10.3390/mi13091549 .

Martinović, Rajko, Joksimović, Danijela, García-March, José Rafael, Vicente, Nardo, Gačić, Zoran, "Evaluation of Physiological State of Pen Shell Pinna nobilis (Linnaeus, 1758) by a Non-Invasive Heart Rate Recording under Short-Term Hyposalinity Test" in Micromachines, 13, no. 9 (2022):1549,
https://doi.org/10.3390/mi13091549 . .

TY  - JOUR
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jokanović, Sandra
AU  - Gačić, Zoran
AU  - Joksimović, Danijela
AU  - Durović, Mirko
AU  - Kljajic, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1009
AB  - This study gives an insight in sensitivity of heart rate (Hr) of Mytilus galloprovincialis as a physiological biomarker. Impact of tributyltin chloride (TBT-Cl) on Hr was studied in parallel with evaluation of mutagenic, genotoxic and cytotoxic potential of TBT-Cl (10,100 and 1000 mu g/L) within 96 h treatment in static conditions. Mutagenic potential was assessed by SOS/umuC assay while genotoxicity was assessed in haemocytes of M. galloprovincialis by using the comet assay and the micronucleus test. Benzo(a)pyrene (B(a)P) was used as a positive control. Hr variations detected in TBT-Cl treatments can be linked to data obtained in the genotoxicological assays indicating that Hr can be considered and used as a reliable physiological biomarker for detecting the presence of organotin compounds. However despite the observed genotoxic potential of B(a)P, a noteworthy Hr response was not observed which further questions the potential of Hr in the detection of different types of pollutants.
PB  - Elsevier Science Bv, Amsterdam
T2  - Environmental Toxicology and Pharmacology
T1  - Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride
EP  - 174
SP  - 165
VL  - 47
DO  - 10.1016/j.etap.2016.09.019
ER  - 

@article{
author = "Martinović, Rajko and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jokanović, Sandra and Gačić, Zoran and Joksimović, Danijela and Durović, Mirko and Kljajic, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "This study gives an insight in sensitivity of heart rate (Hr) of Mytilus galloprovincialis as a physiological biomarker. Impact of tributyltin chloride (TBT-Cl) on Hr was studied in parallel with evaluation of mutagenic, genotoxic and cytotoxic potential of TBT-Cl (10,100 and 1000 mu g/L) within 96 h treatment in static conditions. Mutagenic potential was assessed by SOS/umuC assay while genotoxicity was assessed in haemocytes of M. galloprovincialis by using the comet assay and the micronucleus test. Benzo(a)pyrene (B(a)P) was used as a positive control. Hr variations detected in TBT-Cl treatments can be linked to data obtained in the genotoxicological assays indicating that Hr can be considered and used as a reliable physiological biomarker for detecting the presence of organotin compounds. However despite the observed genotoxic potential of B(a)P, a noteworthy Hr response was not observed which further questions the potential of Hr in the detection of different types of pollutants.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Environmental Toxicology and Pharmacology",
title = "Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride",
pages = "174-165",
volume = "47",
doi = "10.1016/j.etap.2016.09.019"
}

Martinović, R., Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Jokanović, S., Gačić, Z., Joksimović, D., Durović, M., Kljajic, Z.,& Vukovic-Gacic, B.. (2016). Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride. in Environmental Toxicology and Pharmacology
Elsevier Science Bv, Amsterdam., 47, 165-174.
https://doi.org/10.1016/j.etap.2016.09.019

Martinović R, Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Jokanović S, Gačić Z, Joksimović D, Durović M, Kljajic Z, Vukovic-Gacic B. Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride. in Environmental Toxicology and Pharmacology. 2016;47:165-174.
doi:10.1016/j.etap.2016.09.019 .

Martinović, Rajko, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jokanović, Sandra, Gačić, Zoran, Joksimović, Danijela, Durović, Mirko, Kljajic, Zoran, Vukovic-Gacic, Branka, "Comparative assessment of cardiac activity and DNA damage in haemocytes of the Mediterranean mussel Mytilus galloprovincialis in exposure to tributyltin chloride" in Environmental Toxicology and Pharmacology, 47 (2016):165-174,
https://doi.org/10.1016/j.etap.2016.09.019 . .