TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1725
AB  - Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1725
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1725"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4732
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2175
AB  - Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia
PB  - Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2175
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Vuković-Gačić, Branka and Paunović, Momir",
year = "2021",
abstract = "Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia",
publisher = "Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2175"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Vuković-Gačić, B.,& Paunović, M.. (2021). Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Vuković-Gačić B, Paunović M. Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Vuković-Gačić, Branka, Paunović, Momir, "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme." in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2171
AB  - Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.
PB  - Narodna biblioteka Srbije
C3  - 49. konferencija o aktuelnim temama korišćenja i zaštite voda
T1  - Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije
EP  - 40
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2171
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.",
publisher = "Narodna biblioteka Srbije",
journal = "49. konferencija o aktuelnim temama korišćenja i zaštite voda",
title = "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije",
pages = "40-37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2171"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda
Narodna biblioteka Srbije., 37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda. 2020;:37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije" in 49. konferencija o aktuelnim temama korišćenja i zaštite voda (2020):37-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .