TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Vojnović Milutinović, Danijela
AU  - Jovanović-Marić, Jovana
AU  - K.T. Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1566
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters
SP  - 156964
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Vojnović Milutinović, Danijela and Jovanović-Marić, Jovana and K.T. Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukic, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters",
pages = "156964",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Vojnović Milutinović, D., Jovanović-Marić, J., K.T. Kirschner, A., Farnleitner, A., Linke, R., Đukic, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment
Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Vojnović Milutinović D, Jovanović-Marić J, K.T. Kirschner A, Farnleitner A, Linke R, Đukic A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Vojnović Milutinović, Danijela, Jovanović-Marić, Jovana, K.T. Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukic, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters" in Science of the Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kracun-Kolarević, Margareta
AU  - Lundy, Lian
AU  - Kirschner, Alexander K. T.
AU  - Farnleitner, Andreas
AU  - Djukic, Aleksandar
AU  - Colic, Jasna
AU  - Nenin, Tanja
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1489
AB  - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nudeocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 x 10(3) to 132 x 10(4) copies,1) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
VL  - 783
DO  - 10.1016/j.scitotenv.2021.146967
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kracun-Kolarević, Margareta and Lundy, Lian and Kirschner, Alexander K. T. and Farnleitner, Andreas and Djukic, Aleksandar and Colic, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir",
year = "2021",
abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nudeocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 x 10(3) to 132 x 10(4) copies,1) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters",
volume = "783",
doi = "10.1016/j.scitotenv.2021.146967"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kracun-Kolarević, M., Lundy, L., Kirschner, A. K. T., Farnleitner, A., Djukic, A., Colic, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment
Elsevier, Amsterdam., 783.
https://doi.org/10.1016/j.scitotenv.2021.146967

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kracun-Kolarević M, Lundy L, Kirschner AKT, Farnleitner A, Djukic A, Colic J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment. 2021;783.
doi:10.1016/j.scitotenv.2021.146967 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kracun-Kolarević, Margareta, Lundy, Lian, Kirschner, Alexander K. T., Farnleitner, Andreas, Djukic, Aleksandar, Colic, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of the Total Environment, 783 (2021),
https://doi.org/10.1016/j.scitotenv.2021.146967 . .

TY  - JOUR
AU  - Jovanović-Maric, Jovana M.
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Smiljanic, Petar B.
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1369
AB  - This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers
EP  - 229
IS  - 2
SP  - 224
VL  - 105
DO  - 10.1007/s00128-020-02944-4
ER  - 

@article{
author = "Jovanović-Maric, Jovana M. and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Smiljanic, Petar B. and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2020",
abstract = "This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers",
pages = "229-224",
number = "2",
volume = "105",
doi = "10.1007/s00128-020-02944-4"
}

Jovanović-Maric, J. M., Kracun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Smiljanic, P. B., Gačić, Z.,& Vukovic-Gacic, B.. (2020). Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 105(2), 224-229.
https://doi.org/10.1007/s00128-020-02944-4

Jovanović-Maric JM, Kracun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Smiljanic PB, Gačić Z, Vukovic-Gacic B. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology. 2020;105(2):224-229.
doi:10.1007/s00128-020-02944-4 .

Jovanović-Maric, Jovana M., Kracun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Smiljanic, Petar B., Gačić, Zoran, Vukovic-Gacic, Branka, "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers" in Bulletin of Environmental Contamination and Toxicology, 105, no. 2 (2020):224-229,
https://doi.org/10.1007/s00128-020-02944-4 . .

TY  - CHAP
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, J.
AU  - Đorđević Aleksić, Jelena
AU  - Ilić, M.
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Divac, Rankov, A.
AU  - Ilić, B.
AU  - Pešić, V.
AU  - Vukovic-Gacic, Branka
AU  - Paunović, Momir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1302
AB  - Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Impact of pollution on rivers in Montenegro: Ecotoxicological perspective
EP  - 133
SP  - 111
VL  - 93
DO  - 10.1007/698_2019_425
ER  - 

@inbook{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, J. and Đorđević Aleksić, Jelena and Ilić, M. and Sunjog, Karolina and Kostić-Vuković, Jovana and Divac, Rankov, A. and Ilić, B. and Pešić, V. and Vukovic-Gacic, Branka and Paunović, Momir",
year = "2020",
abstract = "Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective",
pages = "133-111",
volume = "93",
doi = "10.1007/698_2019_425"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević Aleksić, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac, R. A., Ilić, B., Pešić, V., Vukovic-Gacic, B.,& Paunović, M.. (2020). Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 111-133.
https://doi.org/10.1007/698_2019_425

Kracun-Kolarević M, Kolarević S, Jovanović J, Đorđević Aleksić J, Ilić M, Sunjog K, Kostić-Vuković J, Divac RA, Ilić B, Pešić V, Vukovic-Gacic B, Paunović M. Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry. 2020;93:111-133.
doi:10.1007/698_2019_425 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, J., Đorđević Aleksić, Jelena, Ilić, M., Sunjog, Karolina, Kostić-Vuković, Jovana, Divac, Rankov, A., Ilić, B., Pešić, V., Vukovic-Gacic, Branka, Paunović, Momir, "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective" in Handbook of Environmental Chemistry, 93 (2020):111-133,
https://doi.org/10.1007/698_2019_425 . .

TY  - JOUR
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Deutschmann, Bjorn
AU  - Hollert, Henner
AU  - Tenji, Dina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1362
AB  - In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments
EP  - 33915
IS  - 27
SP  - 33903
VL  - 27
DO  - 10.1007/s11356-020-09597-0
ER  - 

@article{
author = "Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Sunjog, Karolina and Kostić-Vuković, Jovana and Deutschmann, Bjorn and Hollert, Henner and Tenji, Dina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2020",
abstract = "In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments",
pages = "33915-33903",
number = "27",
volume = "27",
doi = "10.1007/s11356-020-09597-0"
}

Jovanović-Marić, J., Kracun-Kolarević, M., Kolarević, S., Sunjog, K., Kostić-Vuković, J., Deutschmann, B., Hollert, H., Tenji, D., Paunović, M.,& Vukovic-Gacic, B.. (2020). Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(27), 33903-33915.
https://doi.org/10.1007/s11356-020-09597-0

Jovanović-Marić J, Kracun-Kolarević M, Kolarević S, Sunjog K, Kostić-Vuković J, Deutschmann B, Hollert H, Tenji D, Paunović M, Vukovic-Gacic B. Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research. 2020;27(27):33903-33915.
doi:10.1007/s11356-020-09597-0 .

Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Kolarević, Stoimir, Sunjog, Karolina, Kostić-Vuković, Jovana, Deutschmann, Bjorn, Hollert, Henner, Tenji, Dina, Paunović, Momir, Vukovic-Gacic, Branka, "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments" in Environmental Science and Pollution Research, 27, no. 27 (2020):33903-33915,
https://doi.org/10.1007/s11356-020-09597-0 . .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Jovanović, J.
AU  - Ilić, M.
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, S.
AU  - Joksimović, D.
AU  - Pešić, V.
AU  - Kirschner, A.K.T.
AU  - Linke, R.
AU  - Ixenmaier, S.
AU  - Farnleitner, Andreas
AU  - Savio, D.
AU  - Reischer, Georg
AU  - Tomić, N.
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1310
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Microbiological water quality of rivers in Montenegro
EP  - 155
SP  - 135
VL  - 93
DO  - 10.1007/698_2019_420
ER  - 

@inbook{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Jovanović, J. and Ilić, M. and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, S. and Joksimović, D. and Pešić, V. and Kirschner, A.K.T. and Linke, R. and Ixenmaier, S. and Farnleitner, Andreas and Savio, D. and Reischer, Georg and Tomić, N. and Vukovic-Gacic, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbiological water quality of rivers in Montenegro",
pages = "155-135",
volume = "93",
doi = "10.1007/698_2019_420"
}

Kolarević, S., Kracun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vukovic-Gacic, B.. (2020). Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 135-155.
https://doi.org/10.1007/698_2019_420

Kolarević S, Kracun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner A, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vukovic-Gacic B. Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry. 2020;93:135-155.
doi:10.1007/698_2019_420 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Jovanović, J., Ilić, M., Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, Andreas, Savio, D., Reischer, Georg, Tomić, N., Vukovic-Gacic, Branka, "Microbiological water quality of rivers in Montenegro" in Handbook of Environmental Chemistry, 93 (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - JOUR
AU  - Andjus, Stefan
AU  - Nikolic, Nadja
AU  - Dobricic, Valerija
AU  - Marjanović, An
AU  - Gačić, Zoran
AU  - Branković, Goran
AU  - Aković, Maja R.
AU  - Paunović, Momir
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1134
AB  - Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.
PB  - Pagepress Publ, Pavia
T2  - Journal of Limnology
T1  - Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study
EP  - 208
IS  - 2
SP  - 199
VL  - 77
DO  - 10.4081/jlimnol.2017.1677
ER  - 

@article{
author = "Andjus, Stefan and Nikolic, Nadja and Dobricic, Valerija and Marjanović, An and Gačić, Zoran and Branković, Goran and Aković, Maja R. and Paunović, Momir",
year = "2018",
abstract = "Sponges in the large rivers within the Danube River Basin (DRB) have not been adequately studied. Hence. the aim of this work was to undertake an investigation on the distribution of sponge species in the Danube and Sava rivers. Out of SS localities covered by the study, sponges were found at 25 sites only (46 samples in total). By using morpholotical (light and scanning electron microscopy) and genetic (28S rDNA sequencing) analysis. four species were determined: Ephydatia fluviaulis (Linnaeus, 1759), Spongilla lacustris (Linnaeus, 1759), Eunapius fragilis (Leidy, 1851), Trachospangilla horrida Weltner, 1893. In the Danube.. the predominant species was found to be E. fluviatilis making approximately 80% of collected samples, while in the Sava River S. lacustris dominated, representing 46% of the river sponges. Our work represents one of the few studies on freshwater sponges within the DRB from long stretches of the large lowland rivers (more than 2500 km of the Danube River and about 900 km of the Sava River). Moreover, molecular analysis for the identification of freshwater sponges was applied on the material collected from a wide area. thus contributing to the systematic studies on the distribution and abundance of the European freshwater invertebrate fauna in general.",
publisher = "Pagepress Publ, Pavia",
journal = "Journal of Limnology",
title = "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study",
pages = "208-199",
number = "2",
volume = "77",
doi = "10.4081/jlimnol.2017.1677"
}

Andjus, S., Nikolic, N., Dobricic, V., Marjanović, A., Gačić, Z., Branković, G., Aković, M. R.,& Paunović, M.. (2018). Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology
Pagepress Publ, Pavia., 77(2), 199-208.
https://doi.org/10.4081/jlimnol.2017.1677

Andjus S, Nikolic N, Dobricic V, Marjanović A, Gačić Z, Branković G, Aković MR, Paunović M. Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study. in Journal of Limnology. 2018;77(2):199-208.
doi:10.4081/jlimnol.2017.1677 .

Andjus, Stefan, Nikolic, Nadja, Dobricic, Valerija, Marjanović, An, Gačić, Zoran, Branković, Goran, Aković, Maja R., Paunović, Momir, "Contribution to the knowledge on the distribution of freshwater sponges - the Danube and Sava rivers case study" in Journal of Limnology, 77, no. 2 (2018):199-208,
https://doi.org/10.4081/jlimnol.2017.1677 . .

TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milosković, Aleksandra
AU  - Radojković, Nataša
AU  - Simic, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1163
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
EP  - 1299
SP  - 1289
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milosković, Aleksandra and Radojković, Nataša and Simic, Vladica and Dojčinović, Biljana and Kracun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
pages = "1299-1289",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanović, J., Kolarević, S., Milosković, A., Radojković, N., Simic, V., Dojčinović, B., Kracun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Đorđević Aleksić, J., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milosković A, Radojković N, Simic V, Dojčinović B, Kracun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijic J, Đorđević Aleksić J, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milosković, Aleksandra, Radojković, Nataša, Simic, Vladica, Dojčinović, Biljana, Kracun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1160
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .
PB  - Springer International Publishing Ag, Cham
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
EP  - 712
IS  - 5
SP  - 703
VL  - 12
DO  - 10.1007/s41742-018-0127-6
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simic, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .",
publisher = "Springer International Publishing Ag, Cham",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
pages = "712-703",
number = "5",
volume = "12",
doi = "10.1007/s41742-018-0127-6"
}

Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simic, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vukovic-Gacic, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research
Springer International Publishing Ag, Cham., 12(5), 703-712.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kracun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simic V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vukovic-Gacic B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simic, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vukovic-Gacic, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712,
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kirschner, Alexander K. T.
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Gačić, Zoran
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1719
AB  - The level of genotoxic pollution was assessed along the Danube River and its most significant tributary Sava River by measuring the level of DNA damage in aquatic organisms collected from the selected sites. The Danube River survey was conducted within the Joint Danube Survey 3 project in 2013 on 34 sites along the 2285 rkm using mussels (Unio sp.) and fish (Alburnus alburnus) as bioindicators. The Sava River survey was conducted within the Globaqua project in 2015 at 12 sites along 900 rkm using fish (A. alburnus/Alburnoides bipunctatus) as bioindicators. The level of DNA damage was evaluated by the comet assay in haemocytes of mussels and blood cells of fish. The level of faecal pollution along the rivers was studied in parallel with genotoxicological surveys by using the standard indicators, total coliforms, Escherichia coli and Enterococci. Quantification was performed with Colilert/Enterolert Quanti-Tray 2000 and MPN approach. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes faecal markers have been used for tracking the source of pollution (microbial source tracking -MST). The human-associated BacHum and HF183II, the ruminantassociated BacR and the pig-associated Pig2Bac fecal markers were selected. The hotspots of faecal pollution were detected at both rivers. Presence of pollution was especially evident in the countries in which the legislation related to wastewater treatment and management is not fully implemented. In the case of the Danube River the most critical section of the river was the Pannonian plain (sector VI) while in the case of the Sava River the most affected section was the lower stretch of the river. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II in the majority of the analyzed samples. High correlation was observed between the standard fecal indicators and human associated faecal markers. Within the Danube survey, the highest levels of DNA damage were recorded in organisms from the section
VI, which is under the impact of untreated wastewater discharges. In 2013 the Sava River was characterized with a lower level of both faecal and genotoxic pollution in comparison with the Danube. Similar observations were found within the Sava River survey in 2015 where the level of DNA damage in fish specimens from Sava was lower in comparison with the samples from the Danube. At both rivers detected genotoxic potential was traceable to the deterioration of quality by communal and industrial wastewaters. Acknowledgements: International Commission for the Protection of the Danube River, EU Seventh Framework Program GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys
SP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1719
ER  - 

@conference{
author = "Kolarević, Stoimir and Kirschner, Alexander K. T. and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Gačić, Zoran and Farnleitner, Andreas and Reischer, Georg and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "The level of genotoxic pollution was assessed along the Danube River and its most significant tributary Sava River by measuring the level of DNA damage in aquatic organisms collected from the selected sites. The Danube River survey was conducted within the Joint Danube Survey 3 project in 2013 on 34 sites along the 2285 rkm using mussels (Unio sp.) and fish (Alburnus alburnus) as bioindicators. The Sava River survey was conducted within the Globaqua project in 2015 at 12 sites along 900 rkm using fish (A. alburnus/Alburnoides bipunctatus) as bioindicators. The level of DNA damage was evaluated by the comet assay in haemocytes of mussels and blood cells of fish. The level of faecal pollution along the rivers was studied in parallel with genotoxicological surveys by using the standard indicators, total coliforms, Escherichia coli and Enterococci. Quantification was performed with Colilert/Enterolert Quanti-Tray 2000 and MPN approach. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes faecal markers have been used for tracking the source of pollution (microbial source tracking -MST). The human-associated BacHum and HF183II, the ruminantassociated BacR and the pig-associated Pig2Bac fecal markers were selected. The hotspots of faecal pollution were detected at both rivers. Presence of pollution was especially evident in the countries in which the legislation related to wastewater treatment and management is not fully implemented. In the case of the Danube River the most critical section of the river was the Pannonian plain (sector VI) while in the case of the Sava River the most affected section was the lower stretch of the river. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II in the majority of the analyzed samples. High correlation was observed between the standard fecal indicators and human associated faecal markers. Within the Danube survey, the highest levels of DNA damage were recorded in organisms from the section
VI, which is under the impact of untreated wastewater discharges. In 2013 the Sava River was characterized with a lower level of both faecal and genotoxic pollution in comparison with the Danube. Similar observations were found within the Sava River survey in 2015 where the level of DNA damage in fish specimens from Sava was lower in comparison with the samples from the Danube. At both rivers detected genotoxic potential was traceable to the deterioration of quality by communal and industrial wastewaters. Acknowledgements: International Commission for the Protection of the Danube River, EU Seventh Framework Program GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys",
pages = "51",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1719"
}

Kolarević, S., Kirschner, A. K. T., Kracun-Kolarević, M., Kostić-Vuković, J., Gačić, Z., Farnleitner, A., Reischer, G., Paunović, M.,& Vuković-Gačić, B.. (2018). Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 51.
https://hdl.handle.net/21.15107/rcub_rimsi_1719

Kolarević S, Kirschner AKT, Kracun-Kolarević M, Kostić-Vuković J, Gačić Z, Farnleitner A, Reischer G, Paunović M, Vuković-Gačić B. Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:51.
https://hdl.handle.net/21.15107/rcub_rimsi_1719 .

Kolarević, Stoimir, Kirschner, Alexander K. T., Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Gačić, Zoran, Farnleitner, Andreas, Reischer, Georg, Paunović, Momir, Vuković-Gačić, Branka, "Identification of hotspots of genotoxicological and faecal pollution along the Danube and Sava rivers – the whole river surveys" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):51,
https://hdl.handle.net/21.15107/rcub_rimsi_1719 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1060
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - JOUR
AU  - Macic, V.
AU  - Mandic, M.
AU  - Pestoric, B.
AU  - Gačić, Zoran
AU  - Paunović, Momir
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1026
AB  - The typology and quantity of the marine litter was investigated in the shallow coastal area of southeast Adriatic Sea (Montenegro). Surveys were performed during the period 2012-2014 and 47 sites were analysed during underwater visual surveys by SCUBA diving. Litter items were classified in seven categories (plastic, metal, rubber, paper, clothing, glass and others) according to the nature of the material. Plastic litter was dominant (54%) followed by metal items (23%), and the density of marine litter was from 0 to 16.6/1000m(2) with an average of 2.49/1000m(2). As expected, a greater abundance of marine litter was found in the Boka Kotorska Bay and close to urbanised areas.
PB  - Parlar Scientific Publications
T2  - Fresenius Environmental Bulletin
T1  - First assessment of marine litter in shallow south-east Adriatic sea
EP  - 4840
IS  - 7
SP  - 4834
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1026
ER  - 

@article{
author = "Macic, V. and Mandic, M. and Pestoric, B. and Gačić, Zoran and Paunović, Momir",
year = "2017",
abstract = "The typology and quantity of the marine litter was investigated in the shallow coastal area of southeast Adriatic Sea (Montenegro). Surveys were performed during the period 2012-2014 and 47 sites were analysed during underwater visual surveys by SCUBA diving. Litter items were classified in seven categories (plastic, metal, rubber, paper, clothing, glass and others) according to the nature of the material. Plastic litter was dominant (54%) followed by metal items (23%), and the density of marine litter was from 0 to 16.6/1000m(2) with an average of 2.49/1000m(2). As expected, a greater abundance of marine litter was found in the Boka Kotorska Bay and close to urbanised areas.",
publisher = "Parlar Scientific Publications",
journal = "Fresenius Environmental Bulletin",
title = "First assessment of marine litter in shallow south-east Adriatic sea",
pages = "4840-4834",
number = "7",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1026"
}

Macic, V., Mandic, M., Pestoric, B., Gačić, Z.,& Paunović, M.. (2017). First assessment of marine litter in shallow south-east Adriatic sea. in Fresenius Environmental Bulletin
Parlar Scientific Publications., 26(7), 4834-4840.
https://hdl.handle.net/21.15107/rcub_rimsi_1026

Macic V, Mandic M, Pestoric B, Gačić Z, Paunović M. First assessment of marine litter in shallow south-east Adriatic sea. in Fresenius Environmental Bulletin. 2017;26(7):4834-4840.
https://hdl.handle.net/21.15107/rcub_rimsi_1026 .

Macic, V., Mandic, M., Pestoric, B., Gačić, Zoran, Paunović, Momir, "First assessment of marine litter in shallow south-east Adriatic sea" in Fresenius Environmental Bulletin, 26, no. 7 (2017):4834-4840,
https://hdl.handle.net/21.15107/rcub_rimsi_1026 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1014
AB  - The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.
PB  - Wiley, Hoboken
T2  - Clean-Soil Air Water
T1  - Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus
EP  - 1476
IS  - 11
SP  - 1471
VL  - 44
DO  - 10.1002/clen.201500482
ER  - 

@article{
author = "Kolarević, Stoimir and Gačić, Zoran and Kostić-Vuković, Jovana and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.",
publisher = "Wiley, Hoboken",
journal = "Clean-Soil Air Water",
title = "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus",
pages = "1476-1471",
number = "11",
volume = "44",
doi = "10.1002/clen.201500482"
}

Kolarević, S., Gačić, Z., Kostić-Vuković, J., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water
Wiley, Hoboken., 44(11), 1471-1476.
https://doi.org/10.1002/clen.201500482

Kolarević S, Gačić Z, Kostić-Vuković J, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water. 2016;44(11):1471-1476.
doi:10.1002/clen.201500482 .

Kolarević, Stoimir, Gačić, Zoran, Kostić-Vuković, Jovana, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus" in Clean-Soil Air Water, 44, no. 11 (2016):1471-1476,
https://doi.org/10.1002/clen.201500482 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Milosković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1007
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Simonović, Predrag and Simic, Vladica and Milosković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450"
}

Kolarević, S., Aborgiba, M., Kracun-Kolarević, M., Kostić-Vuković, J., Simonović, P., Simic, V., Milosković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kracun-Kolarević M, Kostić-Vuković J, Simonović P, Simic V, Milosković A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Simonović, Predrag, Simic, Vladica, Milosković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .

TY  - JOUR
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Marković, Vanja
AU  - Illic, Marija
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Gačić, Zoran
AU  - Diamantini, Elena
AU  - Stella, Elisa
AU  - Petrović, Mira
AU  - Majone, Bruno
AU  - Bellin, Alberto
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/977
AB  - In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
EP  - 1391
SP  - 1383
VL  - 571
DO  - 10.1016/j.scitotenv.2016.07.099
ER  - 

@article{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, Jovana and Marković, Vanja and Illic, Marija and Simonović, Predrag and Simic, Vladica and Gačić, Zoran and Diamantini, Elena and Stella, Elisa and Petrović, Mira and Majone, Bruno and Bellin, Alberto and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin",
pages = "1391-1383",
volume = "571",
doi = "10.1016/j.scitotenv.2016.07.099"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Marković, V., Illic, M., Simonović, P., Simic, V., Gačić, Z., Diamantini, E., Stella, E., Petrović, M., Majone, B., Bellin, A., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 571, 1383-1391.
https://doi.org/10.1016/j.scitotenv.2016.07.099

Kracun-Kolarević M, Kolarević S, Jovanović J, Marković V, Illic M, Simonović P, Simic V, Gačić Z, Diamantini E, Stella E, Petrović M, Majone B, Bellin A, Paunović M, Vukovic-Gacic B. Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment. 2016;571:1383-1391.
doi:10.1016/j.scitotenv.2016.07.099 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, Jovana, Marković, Vanja, Illic, Marija, Simonović, Predrag, Simic, Vladica, Gačić, Zoran, Diamantini, Elena, Stella, Elisa, Petrović, Mira, Majone, Bruno, Bellin, Alberto, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin" in Science of the Total Environment, 571 (2016):1383-1391,
https://doi.org/10.1016/j.scitotenv.2016.07.099 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1016
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
EP  - 1471
SP  - 1460
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
ER  - 

@article{
author = "Vrzel, Janja and Vukovic-Gacic, Branka and Kolarević, Stoimir and Gačić, Zoran and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
pages = "1471-1460",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213"
}

Vrzel, J., Vukovic-Gacic, B., Kolarević, S., Gačić, Z., Kracun-Kolarević, M., Kostić-Vuković, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vukovic-Gacic B, Kolarević S, Gačić Z, Kracun-Kolarević M, Kostić-Vuković J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vukovic-Gacic, Branka, Kolarević, Stoimir, Gačić, Zoran, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of the Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Slobodnik, Jaroslav
AU  - Liska, Igor
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/970
AB  - In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3
EP  - 385
SP  - 377
VL  - 540
DO  - 10.1016/j.scitotenv.2015.06.061
ER  - 

@article{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Slobodnik, Jaroslav and Liska, Igor and Gačić, Zoran and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we assessed the level of genotoxic pollution along the Danube River by measuring the level of DNA damage in the haemocytes of freshwater mussels of Unio sp. (Unio pictorum/Unio tumidus) and Sinanodonta woodiana. The comet assay was used for the assessment of DNA damage. The research was performed on 34 out of 68 sites analysed within the Joint Danube Survey 3 the world's biggest river research expedition of its kind in 2013. During research, 2285 river kilometres were covered with an average distance of 68 km between the sites. The complex data set on concentrations of various substances present in water, suspended particulate matter and sediment on investigated sites gave the opportunity to identify the groups of xenobiotics which mostly affect the studied biomarker DNA damage. The highest levels of DNA damage were recorded in the section VI (Panonnian Plain), which is under the impact of untreated wastewater discharges. Both positive and negative influences of the large tributaries on the level of genotoxicity in the Danube River were evident. Significant correlation in response was detected between the studied species of freshwater mussels. The level of DNA damage in mussels correlated with concentrations of compounds from the group of hazardous priority substances (polycyclic aromatic hydrocarbons), persistent organic pollutants (dioxins) and emerging pollutants (Oxazepam, Chloridazon-desphenyl).",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3",
pages = "385-377",
volume = "540",
doi = "10.1016/j.scitotenv.2015.06.061"
}

Kolarević, S., Kracun-Kolarević, M., Kostić-Vuković, J., Slobodnik, J., Liska, I., Gačić, Z., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment
Elsevier, Amsterdam., 540, 377-385.
https://doi.org/10.1016/j.scitotenv.2015.06.061

Kolarević S, Kracun-Kolarević M, Kostić-Vuković J, Slobodnik J, Liska I, Gačić Z, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3. in Science of the Total Environment. 2016;540:377-385.
doi:10.1016/j.scitotenv.2015.06.061 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Slobodnik, Jaroslav, Liska, Igor, Gačić, Zoran, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Assessment of the genotoxic potential along the Danube River by application of the comet assay on haemocytes of freshwater mussels: The Joint Danube Survey 3" in Science of the Total Environment, 540 (2016):377-385,
https://doi.org/10.1016/j.scitotenv.2015.06.061 . .

TY  - JOUR
AU  - Deutschmann, Bjorn
AU  - Kolarević, Stoimir
AU  - Brack, Werner
AU  - Kaisarević, Sonja
AU  - Kostić-Vuković, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Liska, Igor
AU  - Paunović, Momir
AU  - Seiler, Thomas-Benjamin
AU  - Shao, Ying
AU  - Sipos, Sandor
AU  - Slobodnik, Jaroslav
AU  - Teodorović, Ivana
AU  - Vukovic-Gacic, Branka
AU  - Hollert, Henner
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/957
AB  - The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
EP  - 1449
SP  - 1441
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.175
ER  - 

@article{
author = "Deutschmann, Bjorn and Kolarević, Stoimir and Brack, Werner and Kaisarević, Sonja and Kostić-Vuković, Jovana and Kracun-Kolarević, Margareta and Liska, Igor and Paunović, Momir and Seiler, Thomas-Benjamin and Shao, Ying and Sipos, Sandor and Slobodnik, Jaroslav and Teodorović, Ivana and Vukovic-Gacic, Branka and Hollert, Henner",
year = "2016",
abstract = "The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay",
pages = "1449-1441",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.175"
}

Deutschmann, B., Kolarević, S., Brack, W., Kaisarević, S., Kostić-Vuković, J., Kracun-Kolarević, M., Liska, I., Paunović, M., Seiler, T., Shao, Y., Sipos, S., Slobodnik, J., Teodorović, I., Vukovic-Gacic, B.,& Hollert, H.. (2016). Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1441-1449.
https://doi.org/10.1016/j.scitotenv.2016.07.175

Deutschmann B, Kolarević S, Brack W, Kaisarević S, Kostić-Vuković J, Kracun-Kolarević M, Liska I, Paunović M, Seiler T, Shao Y, Sipos S, Slobodnik J, Teodorović I, Vukovic-Gacic B, Hollert H. Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment. 2016;573:1441-1449.
doi:10.1016/j.scitotenv.2016.07.175 .

Deutschmann, Bjorn, Kolarević, Stoimir, Brack, Werner, Kaisarević, Sonja, Kostić-Vuković, Jovana, Kracun-Kolarević, Margareta, Liska, Igor, Paunović, Momir, Seiler, Thomas-Benjamin, Shao, Ying, Sipos, Sandor, Slobodnik, Jaroslav, Teodorović, Ivana, Vukovic-Gacic, Branka, Hollert, Henner, "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay" in Science of the Total Environment, 573 (2016):1441-1449,
https://doi.org/10.1016/j.scitotenv.2016.07.175 . .

TY  - JOUR
AU  - Aborgiba, Mustafa
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Elbahi, Samia
AU  - Knezevic-Vukcević, Jelena
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/936
AB  - Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River
EP  - 367
SP  - 358
VL  - 540
DO  - 10.1016/j.scitotenv.2015.03.120
ER  - 

@article{
author = "Aborgiba, Mustafa and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Elbahi, Samia and Knezevic-Vukcević, Jelena and Lenhardt, Mirjana and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2016",
abstract = "Extreme hydrological events, such as water scarcity and flooding, can modify the effect of other stressors present in aquatic environment, which could result in the significant changes in the ecosystem functioning. Presence and interaction of various stressors (genotoxic pollutants) in the environment can influence the integrity of DNA molecules in aquatic organisms which can be negatively reflected on the individual, population and community levels. Therefore, in this study we have investigated the impact of flooding, in terms of genotoxicity, on organisms belonging to different trophic levels. The study was carried out on the site situated in the lower stretch of the Sava River which faced devastating effects of severe flooding in May 2014. The flooding occurred during our field experiment and this event provided a unique opportunity to assess its influence to the environment. The in situ effects of this specific situation were monitored by measuring physical, chemical and microbiological parameters of water, and by comparing the level of DNA damage in coelomocytes and haemocytes of freshwater worms Branchiura sowerbyi, haemocytes of freshwater mussels Unto tumidus and blood cells of freshwater fish Abramis bjoerkna/Abramis sapa, by means of the comet assay. Our study indicated that the flooding had a significant impact on water quality by decreasing the amount and discharge rate of urban wastewaters but simultaneously introducing contaminants from the nearby fly ash disposal field into river by runoff, which had diverse effects on the level of DNA damage in the studied organisms. This indicates that the assessment of genotoxic pollution in situ is strongly affected by the choice of the bioindicator organism.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River",
pages = "367-358",
volume = "540",
doi = "10.1016/j.scitotenv.2015.03.120"
}

Aborgiba, M., Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Elbahi, S., Knezevic-Vukcević, J., Lenhardt, M., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2016). Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment
Elsevier, Amsterdam., 540, 358-367.
https://doi.org/10.1016/j.scitotenv.2015.03.120

Aborgiba M, Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Elbahi S, Knezevic-Vukcević J, Lenhardt M, Paunović M, Gačić Z, Vukovic-Gacic B. Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River. in Science of the Total Environment. 2016;540:358-367.
doi:10.1016/j.scitotenv.2015.03.120 .

Aborgiba, Mustafa, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Elbahi, Samia, Knezevic-Vukcević, Jelena, Lenhardt, Mirjana, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Flooding modifies the genotoxic effects of pollution on a worm, a mussel and two fish species from the Sava River" in Science of the Total Environment, 540 (2016):358-367,
https://doi.org/10.1016/j.scitotenv.2015.03.120 . .

TY  - CHAP
AU  - Kapetanović, D.
AU  - Vardić, Smrzlić, I.
AU  - Valić, D.
AU  - Teskeredžić, E.
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Kracun-Kolarević, Margareta
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/929
AB  - Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.
PB  - Springer Verlag
T2  - Handbook of Environmental Chemistry
T1  - Microbial Characterisation of the Sava River
EP  - 228
SP  - 201
VL  - 31
DO  - 10.1007/978-3-662-44034-6_9
ER  - 

@inbook{
author = "Kapetanović, D. and Vardić, Smrzlić, I. and Valić, D. and Teskeredžić, E. and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Kracun-Kolarević, Margareta and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2015",
abstract = "Data on the microbiological quality of the freshwater systems under the anthropogenic influence, such as the Sava River, are of the major importance for the water resource management. Furthermore, analyses of the microbial quality of fish meat provide information of the fish as a valuable food resource from the investigated river basin. The health status of the fish, including dynamics of infection and biodiversity of endoparasites, is important bioindicator of changes in the ecosystem structure and function. For the ecosystem-based approach to the Sava River management, investigations of microbiological quality of the Sava River water and the meat of the European chub as the bioindicator organism, as well as dynamics of infection/biodiversity of intestinal parasites Acanthocephala, were performed. The survey comprised the data collected in periods 2005, 2006 and 2012. Microbiological investigation of water was performed in 2006 and 2012, while microbiological analyses of fish meat and ichthyo-parasitological investigation took place during 2005–2006. A high number of heterotrophic bacteria were recorded during 2006 survey, confirmed by the distinctly higher values of the three faecal indicators (total coliform, E. coli and enterococci), and indicated poor water quality downstream of the cities Zagreb and Velika Gorica, as a result of the municipal sewage outlets. The results from 2012 survey indicated the existence of moderate to critical faecal and organic pollution in all samples. Accumulation of the bacteria in the European chub meat was mainly uniform along the watercourse within standards and limitations for the human consumption. Sampling sites downstream cities of Zagreb and Velika Gorica were characterised with the lower prevalence and abundance of two common species of the chub intestinal acanthocephalan parasites, Pomphorhynchus laevis and Acanthocephalus anguillae. Poor microbiological quality of the water and lower distribution of chub intestinal parasites were related to the anthropogenic influence, downstream of the urban areas.",
publisher = "Springer Verlag",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbial Characterisation of the Sava River",
pages = "228-201",
volume = "31",
doi = "10.1007/978-3-662-44034-6_9"
}

Kapetanović, D., Vardić, S. I., Valić, D., Teskeredžić, E., Kolarević, S., Sunjog, K., Tomović, J. M., Kracun-Kolarević, M., Knezevic-Vukcević, J., Paunović, M., Gačić, Z.,& Vukovic-Gacic, B.. (2015). Microbial Characterisation of the Sava River. in Handbook of Environmental Chemistry
Springer Verlag., 31, 201-228.
https://doi.org/10.1007/978-3-662-44034-6_9

Kapetanović D, Vardić SI, Valić D, Teskeredžić E, Kolarević S, Sunjog K, Tomović JM, Kracun-Kolarević M, Knezevic-Vukcević J, Paunović M, Gačić Z, Vukovic-Gacic B. Microbial Characterisation of the Sava River. in Handbook of Environmental Chemistry. 2015;31:201-228.
doi:10.1007/978-3-662-44034-6_9 .

Kapetanović, D., Vardić, Smrzlić, I., Valić, D., Teskeredžić, E., Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Kracun-Kolarević, Margareta, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, Vukovic-Gacic, Branka, "Microbial Characterisation of the Sava River" in Handbook of Environmental Chemistry, 31 (2015):201-228,
https://doi.org/10.1007/978-3-662-44034-6_9 . .

TY  - CHAP
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Kracun-Kolarević, Margareta
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/928
AB  - Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.
PB  - Springer Verlag
T2  - Handbook of Environmental Chemistry
T1  - Genotoxicological Studies of Lower Stretch of the Sava River
EP  - 452
SP  - 437
VL  - 31
DO  - 10.1007/978-3-662-44034-6_16
ER  - 

@inbook{
author = "Vukovic-Gacic, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Kracun-Kolarević, Margareta and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2015",
abstract = "Genotoxicity monitoring of the lower stretch of the Sava River was performed by the combined approach of in situ assessment of genotoxicity and active biomonitoring of two species of mussels from the Unionidae family, Unio pictorum and Unio tumidus. Genotoxic response was studied using comet assay on hemocytes. For active biomonitoring, the mussels were acclimated to controlled laboratory conditions for 10 days and then exposed at two sites in the Sava River in the area of the city of Belgrade. Hemolymph of exposed specimens of each species was taken after 7, 14, and 30 days of exposure. For in situ assessment, the mussels were collected from five sites in the lower flow of the Sava River. The mussels were sampled immediately after the acclimation served as controls in both types of monitoring procedures. The results of our studies indicated the presence of genotoxic pollution at all studied sites at the Sava River. The level of DNA damage varied at different sites depending on the source and level of pollution. The response to genotoxic pollution was evident at the site in the urban area of Belgrade city, as well as at the sites far from the large urban settlements, suggesting that the lower flow of the Sava River is under pollution pressure.",
publisher = "Springer Verlag",
journal = "Handbook of Environmental Chemistry",
booktitle = "Genotoxicological Studies of Lower Stretch of the Sava River",
pages = "452-437",
volume = "31",
doi = "10.1007/978-3-662-44034-6_16"
}

Vukovic-Gacic, B., Kolarević, S., Sunjog, K., Tomović, J. M., Kracun-Kolarević, M., Knezevic-Vukcević, J., Paunović, M.,& Gačić, Z.. (2015). Genotoxicological Studies of Lower Stretch of the Sava River. in Handbook of Environmental Chemistry
Springer Verlag., 31, 437-452.
https://doi.org/10.1007/978-3-662-44034-6_16

Vukovic-Gacic B, Kolarević S, Sunjog K, Tomović JM, Kracun-Kolarević M, Knezevic-Vukcević J, Paunović M, Gačić Z. Genotoxicological Studies of Lower Stretch of the Sava River. in Handbook of Environmental Chemistry. 2015;31:437-452.
doi:10.1007/978-3-662-44034-6_16 .

Vukovic-Gacic, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Kracun-Kolarević, Margareta, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, "Genotoxicological Studies of Lower Stretch of the Sava River" in Handbook of Environmental Chemistry, 31 (2015):437-452,
https://doi.org/10.1007/978-3-662-44034-6_16 . .

TY  - JOUR
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Atanacković, Ana
AU  - Marković, Vanja
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/925
AB  - Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl2) was evaluated in Limnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 mu M), ET (0.004. 0.04, 0.4 and 4 mu M) and CdCl2 (0.004, 0.04, 0.4, 4 and 40 mu M). Mortality of worms was observed only for CdCl2 (4 and 40 mu M). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 mu M), ET (4 mu M) and CdCl2 (0.4 and 4 mu M). All tested substances induced significant increase of DNA damage except 0.004 mu M of ET. L. udekemianus being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 mu M induced DNA damage is similar to ones detected in wastewaters.
PB  - Springer International Publishing Ag, Cham
T2  - Water Air and Soil Pollution
T1  - Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay
IS  - 8
VL  - 226
DO  - 10.1007/s11270-015-2511-6
ER  - 

@article{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Atanacković, Ana and Marković, Vanja and Gačić, Zoran and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2015",
abstract = "Genotoxicity of 5-fluorouracil (5-FU), etoposide (ET) and cadmium chloride (CdCl2) was evaluated in Limnodrilus udekemianus, cosmopolitan tubificid species, by alkaline single-cell gel electrophoresis (comet assay). Groups of 50 individuals were exposed in vivo in water-only short-term (96 h) tests to 5-FU (0.004, 0.04, 0.4, 4 and 40 mu M), ET (0.004. 0.04, 0.4 and 4 mu M) and CdCl2 (0.004, 0.04, 0.4, 4 and 40 mu M). Mortality of worms was observed only for CdCl2 (4 and 40 mu M). Cell viability lower than 70 % was detected for 5-FU (0.4, 4 and 40 mu M), ET (4 mu M) and CdCl2 (0.4 and 4 mu M). All tested substances induced significant increase of DNA damage except 0.004 mu M of ET. L. udekemianus being sensitive to all tested substances indicates that it can be used in ecogenotoxicology studies. Concern should be raised to cytostatics, especially to 5-FU, since concentration of 0.004 mu M induced DNA damage is similar to ones detected in wastewaters.",
publisher = "Springer International Publishing Ag, Cham",
journal = "Water Air and Soil Pollution",
title = "Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay",
number = "8",
volume = "226",
doi = "10.1007/s11270-015-2511-6"
}

Kracun-Kolarević, M., Kolarević, S., Atanacković, A., Marković, V., Gačić, Z., Paunović, M.,& Vukovic-Gacic, B.. (2015). Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay. in Water Air and Soil Pollution
Springer International Publishing Ag, Cham., 226(8).
https://doi.org/10.1007/s11270-015-2511-6

Kracun-Kolarević M, Kolarević S, Atanacković A, Marković V, Gačić Z, Paunović M, Vukovic-Gacic B. Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay. in Water Air and Soil Pollution. 2015;226(8).
doi:10.1007/s11270-015-2511-6 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Atanacković, Ana, Marković, Vanja, Gačić, Zoran, Paunović, Momir, Vukovic-Gacic, Branka, "Effects of 5-Fluorouracil, Etoposide and CdCl2 in Aquatic Oligochaeta Limnodrilus udekemianus Claparede (Tubificidae) Measured by Comet Assay" in Water Air and Soil Pollution, 226, no. 8 (2015),
https://doi.org/10.1007/s11270-015-2511-6 . .

TY  - JOUR
AU  - Gačić, Zoran
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/822
AB  - The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.
PB  - Elsevier Sci Ltd, Oxford
T2  - Environmental Pollution
T1  - The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus
EP  - 150
SP  - 145
VL  - 191
DO  - 10.1016/j.envpol.2014.04.024
ER  - 

@article{
author = "Gačić, Zoran and Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "The impact of in vivo and in vitro exposure to anticancer drug 5-Fluorouracil (5-FU) on the level of DNA damage was evaluated using comet assay on haemocytes of freshwater mussels Unio pictorum and Unio tumidus. For the in vivo experiment, the groups of 5 mussels per concentration were exposed for 72 h to 5-FU (0.04, 0.4, 4, 40 and 100 mu M) with 0.4 mu M being the lowest concentration to induce significant DNA damage. For the in vitro experiment, the primary cultures of haemocytes were treated with 0.04, 0.4, 4 and 40 mu M 5-FU for 22 h and the treatment with CdCl2 was used as a positive control. In contrast to in vivo exposure, 5-FU did not induce significant increase of DNA damage in vitro, possibly because of the absence of haemocytes proliferation in primary cultures.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Environmental Pollution",
title = "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus",
pages = "150-145",
volume = "191",
doi = "10.1016/j.envpol.2014.04.024"
}

Gačić, Z., Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2014). The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution
Elsevier Sci Ltd, Oxford., 191, 145-150.
https://doi.org/10.1016/j.envpol.2014.04.024

Gačić Z, Kolarević S, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in Environmental Pollution. 2014;191:145-150.
doi:10.1016/j.envpol.2014.04.024 .

Gačić, Zoran, Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "The impact of in vivo and in vitro exposure to base analogue 5-FU on the level of DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus" in Environmental Pollution, 191 (2014):145-150,
https://doi.org/10.1016/j.envpol.2014.04.024 . .

TY  - JOUR
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Tomović, Jelena M
AU  - Knezevic-Vukcević, Jelena
AU  - Paunović, Momir
AU  - Gačić, Zoran
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/820
AB  - Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.
PB  - Springer, Dordrecht
T2  - Hydrobiologia
T1  - Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress
EP  - 231
IS  - 1
SP  - 221
VL  - 735
DO  - 10.1007/s10750-013-1513-x
ER  - 

@article{
author = "Vukovic-Gacic, Branka and Kolarević, Stoimir and Sunjog, Karolina and Tomović, Jelena M and Knezevic-Vukcević, Jelena and Paunović, Momir and Gačić, Zoran",
year = "2014",
abstract = "Genotoxic response of freshwater mussels U. tumidus and U. pictorum to environmental stress was studied using comet assay on hemocytes and gill cells. The mussels were acclimated to controlled laboratory conditions for 10 days, and then exposed at 4 sites in the Sava and Danube rivers in the area of the city of Belgrade. Samples of each species were taken after 7, 14, and 30 days of exposure. The mussels sampled immediately after acclimation served as controls. Genotoxic response in both species was induced earlier at sites receiving untreated wastewaters from the city's main collectors (7 days), than at the site receiving only domestic wastewaters from small settlements located upstream from the city (30 days). There was a correlation between the comet tail intensity values in tissues of exposed mussels and the concentrations of zinc, copper, iron, and arsenic at the exposure sites. The genotoxic responses in both tissues of U. pictorum and in hemocytes of U. tumidus were similar, while U. tumidus gill cells failed to exhibit significant genotoxic response at two sites. These findings, together with higher mortality of U. tumidus at the most polluted sites, promote U. pictorum as a model for genotoxicity monitoring in freshwater environments.",
publisher = "Springer, Dordrecht",
journal = "Hydrobiologia",
title = "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress",
pages = "231-221",
number = "1",
volume = "735",
doi = "10.1007/s10750-013-1513-x"
}

Vukovic-Gacic, B., Kolarević, S., Sunjog, K., Tomović, J. M., Knezevic-Vukcević, J., Paunović, M.,& Gačić, Z.. (2014). Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia
Springer, Dordrecht., 735(1), 221-231.
https://doi.org/10.1007/s10750-013-1513-x

Vukovic-Gacic B, Kolarević S, Sunjog K, Tomović JM, Knezevic-Vukcević J, Paunović M, Gačić Z. Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress. in Hydrobiologia. 2014;735(1):221-231.
doi:10.1007/s10750-013-1513-x .

Vukovic-Gacic, Branka, Kolarević, Stoimir, Sunjog, Karolina, Tomović, Jelena M, Knezevic-Vukcević, Jelena, Paunović, Momir, Gačić, Zoran, "Comparative study of the genotoxic response of freshwater mussels Unio tumidus and Unio pictorum to environmental stress" in Hydrobiologia, 735, no. 1 (2014):221-231,
https://doi.org/10.1007/s10750-013-1513-x . .

TY  - JOUR
AU  - Tomović, Jelena M
AU  - Paunović, Momir
AU  - Atanacković, Ana
AU  - Marković, V.
AU  - Gačić, Zoran
AU  - Csányi, B.
AU  - Sinuć, V.
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/742
AB  - The aim of this study is to present the distribution of aquatic molluscs along a 2600 km long stretch of the Danube River based on information obtained during the Second Joint Danube Survey (JDS2) in 2007. The collected data are used to validate the abiotic typology of the Danube River and to determine the extent of variations in mollusc richness and composition that can be explained by the basic habitat characteristics of the sites. The examination of the distribution of mollusc fauna reveals differences between the three main sectors of the river, the Upper, Middle and Lower Danube. The Iron Gate Section was identified as the boundary between the Middle and Lower, Danube. A total of 42 aquatic mollusc species-group taxa of 14 families are detected within the study area. The Middle and Upper sectors are characterised by higher species richness and diversity as compared to the Lower Danube.
PB  - Bulgarian Academy of Sciences
T2  - Acta Zoologica Bulgarica
T1  - Biotic Typology of the Danube River Based on Distribution of Mollusc Fauna as Revealed by the Second Joint Danube Survey (2007)
EP  - 537
IS  - 4
SP  - 527
VL  - 66
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_742
ER  - 

@article{
author = "Tomović, Jelena M and Paunović, Momir and Atanacković, Ana and Marković, V. and Gačić, Zoran and Csányi, B. and Sinuć, V.",
year = "2014",
abstract = "The aim of this study is to present the distribution of aquatic molluscs along a 2600 km long stretch of the Danube River based on information obtained during the Second Joint Danube Survey (JDS2) in 2007. The collected data are used to validate the abiotic typology of the Danube River and to determine the extent of variations in mollusc richness and composition that can be explained by the basic habitat characteristics of the sites. The examination of the distribution of mollusc fauna reveals differences between the three main sectors of the river, the Upper, Middle and Lower Danube. The Iron Gate Section was identified as the boundary between the Middle and Lower, Danube. A total of 42 aquatic mollusc species-group taxa of 14 families are detected within the study area. The Middle and Upper sectors are characterised by higher species richness and diversity as compared to the Lower Danube.",
publisher = "Bulgarian Academy of Sciences",
journal = "Acta Zoologica Bulgarica",
title = "Biotic Typology of the Danube River Based on Distribution of Mollusc Fauna as Revealed by the Second Joint Danube Survey (2007)",
pages = "537-527",
number = "4",
volume = "66",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_742"
}

Tomović, J. M., Paunović, M., Atanacković, A., Marković, V., Gačić, Z., Csányi, B.,& Sinuć, V.. (2014). Biotic Typology of the Danube River Based on Distribution of Mollusc Fauna as Revealed by the Second Joint Danube Survey (2007). in Acta Zoologica Bulgarica
Bulgarian Academy of Sciences., 66(4), 527-537.
https://hdl.handle.net/21.15107/rcub_rimsi_742

Tomović JM, Paunović M, Atanacković A, Marković V, Gačić Z, Csányi B, Sinuć V. Biotic Typology of the Danube River Based on Distribution of Mollusc Fauna as Revealed by the Second Joint Danube Survey (2007). in Acta Zoologica Bulgarica. 2014;66(4):527-537.
https://hdl.handle.net/21.15107/rcub_rimsi_742 .

Tomović, Jelena M, Paunović, Momir, Atanacković, Ana, Marković, V., Gačić, Zoran, Csányi, B., Sinuć, V., "Biotic Typology of the Danube River Based on Distribution of Mollusc Fauna as Revealed by the Second Joint Danube Survey (2007)" in Acta Zoologica Bulgarica, 66, no. 4 (2014):527-537,
https://hdl.handle.net/21.15107/rcub_rimsi_742 .