TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milosković, Aleksandra
AU  - Radojković, Nataša
AU  - Simic, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1163
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
EP  - 1299
SP  - 1289
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milosković, Aleksandra and Radojković, Nataša and Simic, Vladica and Dojčinović, Biljana and Kracun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
pages = "1299-1289",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanović, J., Kolarević, S., Milosković, A., Radojković, N., Simic, V., Dojčinović, B., Kracun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Đorđević Aleksić, J., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milosković A, Radojković N, Simic V, Dojčinović B, Kracun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijic J, Đorđević Aleksić J, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milosković, Aleksandra, Radojković, Nataša, Simic, Vladica, Dojčinović, Biljana, Kracun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vera
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2150
AB  - Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.
PB  - Balkan Society for Microbiology
C3  - 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
T1  - Application of sos/umuc assay in eco/genotoxicology
EP  - 385
SP  - 385
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2150
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vera and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijić, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2017",
abstract = "Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.",
publisher = "Balkan Society for Microbiology",
journal = "10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017",
title = "Application of sos/umuc assay in eco/genotoxicology",
pages = "385-385",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2150"
}

Jovanović Marić, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijić, J., Đorđević Aleksić, J., Gačić, Z.,& Vuković-Gačić, B.. (2017). Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
Balkan Society for Microbiology., 385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150

Jovanović Marić J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijić J, Đorđević Aleksić J, Gačić Z, Vuković-Gačić B. Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017. 2017;:385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vera, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijić, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Vuković-Gačić, Branka, "Application of sos/umuc assay in eco/genotoxicology" in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017 (2017):385-385,
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .