TY  - JOUR
AU  - Kostić, Igor
AU  - Nikolić, Nina
AU  - Milanovic, Slobodan
AU  - Milenkovic, Ivan
AU  - Pavlović, Jelena
AU  - Paravinja, Ana
AU  - Nikolic, Miroslav
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2101
AB  - Beneficial effects of silicon (Si) on plants have primarily been studied in crop species under single stress. Moreover, nutrient acquisition-based responses to combination of biotic and abiotic stresses (a common situation in natural habitats) have rarely been reported, in particular in conjunction with soil amendments with Si. Pedunculate oak (Quercus robur L.), one of the ecologically and economically most important tree species in Europe, is facing a severe decline due to combined stresses, but also problems in assisted regeneration in nurseries. Here, we studied the effect of Si supply on the leaf nutriome, root traits and overall growth of 12-weeks-old oak seedlings exposed to abiotic stress [low phosphorus (P) supply], biotic stress (Phytophthora plurivora root infection), and their combination. The application of Si had the strongest ameliorative effect on growth, root health and root phenome under the most severe stress conditions (i.e., combination of P deficiency and P. plurivora root infection), where it differentially affected the uptake and leaf accumulation in 11 out of 13 analysed nutrients. Silicon supply tended to reverse the pattern of change of some, but not all, leaf nutrients affected by stresses: P, boron (B) and magnesium (Mg) under P deficiency, and P, B and sulphur (S) under pathogen attack, but also nickel (Ni) and molybdenum (Mo) under all three stresses. Surprisingly, Si affected some nutrients that were not changed by a particular stress itself and decreased leaf Mg levels under all the stresses. On the other hand, pathogen attack increased leaf accumulation of Si. This exploratory work presents the complexity of nutrient crosstalk under three stresses, and opens more questions about genetic networks that control plant physiological responses. Practically, we show a potential of Si application to improve P status and root health in oak seedlings, particularly in nurseries.
PB  - Frontiers
T2  - Frontiers in Plant Science
T1  - Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection
IS  - 14
SP  - 1265782.
DO  - 10.3389/fpls.2023.1265782
ER  - 

@article{
author = "Kostić, Igor and Nikolić, Nina and Milanovic, Slobodan and Milenkovic, Ivan and Pavlović, Jelena and Paravinja, Ana and Nikolic, Miroslav",
year = "2023",
abstract = "Beneficial effects of silicon (Si) on plants have primarily been studied in crop species under single stress. Moreover, nutrient acquisition-based responses to combination of biotic and abiotic stresses (a common situation in natural habitats) have rarely been reported, in particular in conjunction with soil amendments with Si. Pedunculate oak (Quercus robur L.), one of the ecologically and economically most important tree species in Europe, is facing a severe decline due to combined stresses, but also problems in assisted regeneration in nurseries. Here, we studied the effect of Si supply on the leaf nutriome, root traits and overall growth of 12-weeks-old oak seedlings exposed to abiotic stress [low phosphorus (P) supply], biotic stress (Phytophthora plurivora root infection), and their combination. The application of Si had the strongest ameliorative effect on growth, root health and root phenome under the most severe stress conditions (i.e., combination of P deficiency and P. plurivora root infection), where it differentially affected the uptake and leaf accumulation in 11 out of 13 analysed nutrients. Silicon supply tended to reverse the pattern of change of some, but not all, leaf nutrients affected by stresses: P, boron (B) and magnesium (Mg) under P deficiency, and P, B and sulphur (S) under pathogen attack, but also nickel (Ni) and molybdenum (Mo) under all three stresses. Surprisingly, Si affected some nutrients that were not changed by a particular stress itself and decreased leaf Mg levels under all the stresses. On the other hand, pathogen attack increased leaf accumulation of Si. This exploratory work presents the complexity of nutrient crosstalk under three stresses, and opens more questions about genetic networks that control plant physiological responses. Practically, we show a potential of Si application to improve P status and root health in oak seedlings, particularly in nurseries.",
publisher = "Frontiers",
journal = "Frontiers in Plant Science",
title = "Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection",
number = "14",
pages = "1265782.",
doi = "10.3389/fpls.2023.1265782"
}

Kostić, I., Nikolić, N., Milanovic, S., Milenkovic, I., Pavlović, J., Paravinja, A.,& Nikolic, M.. (2023). Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection. in Frontiers in Plant Science
Frontiers.(14), 1265782..
https://doi.org/10.3389/fpls.2023.1265782

Kostić I, Nikolić N, Milanovic S, Milenkovic I, Pavlović J, Paravinja A, Nikolic M. Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection. in Frontiers in Plant Science. 2023;(14):1265782..
doi:10.3389/fpls.2023.1265782 .

Kostić, Igor, Nikolić, Nina, Milanovic, Slobodan, Milenkovic, Ivan, Pavlović, Jelena, Paravinja, Ana, Nikolic, Miroslav, "Silicon modifies leaf nutriome and improves growth of oak seedlings exposed to phosphorus deficiency and Phytophthora plurivora infection" in Frontiers in Plant Science, no. 14 (2023):1265782.,
https://doi.org/10.3389/fpls.2023.1265782 . .

TY  - CONF
AU  - Kostić Kravljanac, Ljiljana
AU  - Trailović, Maja
AU  - Pavlović, Jelena
AU  - Kostić, Igor
AU  - Dubljanin, Tijana
AU  - Nikolic, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2135
AB  - Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.
PB  - ISSAG and AgCenter LSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2135
ER  - 

@conference{
author = "Kostić Kravljanac, Ljiljana and Trailović, Maja and Pavlović, Jelena and Kostić, Igor and Dubljanin, Tijana and Nikolic, Miroslav",
year = "2022",
abstract = "Silicon (Si) is the major constituent of soil present in various fractions, i.e., mobile, adsorbed, occluded (in pedogenic oxides and hydroxides), amorphous (biogenic and lithogenic) and crystalline (primary and secondary silicates, and quartz). Different soil factors such as pH, temperatures, microbial activity, the presence of cations, Al/Fe oxides and hydroxides and organic compounds, influence Si transformation, thereby modifying plant availably of Si. Silicon mobility and transformation in the soil have mainly been studied in the context of pedogenesis or biogeochemical Si cycling. However, research on Si mobility, transformation, and plant availability in the rhizosphere is still lacking. Here, we investigated the root potential of white lupine (Lupinus albus L.), known as a phosphorus (P)-efficient model plant (e.g., root release of H+ and carboxylates), to mobilize Si from the soil. Plants were grown in the rhizoboxes filled with low P soil (control) and fertilized with different N-forms (NO3, NH4 and NO3NH4). The control, NO3- and NO3NH4-fertilized plants accumulated significantly lower amounts of Si than the NH4-fertilized ones. All applied N-forms influenced Si availability in the bulk soil, but Si fractions have further been modified in the rhizosphere, what was crucial for Si accumulation in plants. For instance, NO3 supply slightly decreased Si availability in the bulk soil, but lupine plants accumulated a similar amount of Si as the control plants. A strong gradient of decreasing Si concentrations between bulk and rhizosphere soils was observed in mobile, adsorbed, and amorphous biogenic Si pools in the control and in all N treatments, while occluded and lithogenic amorphous Si pools were recalcitrant. Interestingly, a gradient of increasing concentrations of the amorphous biogenic Si pool between bulk and rhizosphere soils was recorded in the NH4 treatment, concomitantly with the strongest rhizosphere acidification.",
publisher = "ISSAG and AgCenter LSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2135"
}

Kostić Kravljanac, L., Trailović, M., Pavlović, J., Kostić, I., Dubljanin, T.,& Nikolic, M.. (2022). Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenter LSU..
https://hdl.handle.net/21.15107/rcub_rimsi_2135

Kostić Kravljanac L, Trailović M, Pavlović J, Kostić I, Dubljanin T, Nikolic M. Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

Kostić Kravljanac, Ljiljana, Trailović, Maja, Pavlović, Jelena, Kostić, Igor, Dubljanin, Tijana, Nikolic, Miroslav, "Effect of N-forms on Silicon Mobilization in the Rhizosphere of White Lupin" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2135 .

TY  - CONF
AU  - Kostić, Igor
AU  - Milenkovic, Ivan
AU  - Nikolić, Nina
AU  - Milanovic, Slobodan
AU  - Kostić Kravljanac, Ljiljana
AU  - Bosnić, Predrag
AU  - Paravinja, Ana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2112
AB  - Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.
PB  - ISSAG and AgCenterLSU
C3  - 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
T1  - Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2112
ER  - 

@conference{
author = "Kostić, Igor and Milenkovic, Ivan and Nikolić, Nina and Milanovic, Slobodan and Kostić Kravljanac, Ljiljana and Bosnić, Predrag and Paravinja, Ana",
year = "2022",
abstract = "Pedunculate oak (Quercus robur L.) is the most abundant deciduous tree species in Europe with high economical and ecological importance. Different species of Phytophthora are considered as one of the most important factors responsible for deterioration of oak forest, causing serious root damage not only in the forest trees, but also in the nurseries. Oak seedlings were grown in plastic pots with extremely low phosphorus (P) soil (1.5 mg kg-1 total P; no available Olsen-P detected). Silicon (Si) and P were supplied as Na2SiO3 (300 mg Si kg-1 dry soil) and KH2PO4 (180 mg P kg-1 dry soil), respectively. Four treatments (-P/-Si, -P+/Si, +P/-Si, and +P/+Si) were used in the experiment. After two months of experiment, a half of the plants in each treatment were root-inoculated with Phytophthora plurivora. After further four weeks, the first symptoms of P. plurivora infection appeared in leaves (e.g., leaf necrosis and wilting). Plants were then carefully removed from the pots, divided into roots and shoots, and the roots were scanned and analyzed by the WinRHIZO® software. Foliar concentrations of Si, P, K, Ca, Mg, B, Cu, Fe, Mn, and Zn were determined by ICP-OES, while the concentrations of N and S were determined by CHNS Analyzer. The addition of Si obviously improved root health status (e.g., decreasing de number of lesions and necrosis intensity) in the infected plants grown under -P conditions, which was followed by an increased foliar P concentration. The Si supply significantly increased the root variables (e.g., total root volume, root length, and area of thin roots) in both -P and +P plants inoculated with P. plurivora. Therefore, P. plurivora infection and supply of P and Si modulated the nutrient uptake and thereby changed the leaf ionomics, especially for infected -P plants supplied with Si (e.g., significantly increased B, Cu, and Si foliar concentrations and decreased Fe, Mn, Ca, Mg, K, and S foliar concentrations). Furthermore, Si fertilization significantly declined loses in plant dry biomass caused by P. plurivora infection and/or P deficiency, showing biomass comparable to non-infected +P plants.",
publisher = "ISSAG and AgCenterLSU",
journal = "8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA",
title = "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2112"
}

Kostić, I., Milenkovic, I., Nikolić, N., Milanovic, S., Kostić Kravljanac, L., Bosnić, P.,& Paravinja, A.. (2022). Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA
ISSAG and AgCenterLSU., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112

Kostić I, Milenkovic I, Nikolić N, Milanovic S, Kostić Kravljanac L, Bosnić P, Paravinja A. Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions. in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA. 2022;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

Kostić, Igor, Milenkovic, Ivan, Nikolić, Nina, Milanovic, Slobodan, Kostić Kravljanac, Ljiljana, Bosnić, Predrag, Paravinja, Ana, "Silicon modulates root phenomics and leaf ionomics in oak under Phytophthora infection and low phosphorus conditions" in 8th International Conference on Silicon in Agriculture, May 23-26, 2022, New Orleans, LA, USA (2022):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2112 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2707
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.
PB  - MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - https://doi.org/10.3390/plants11243587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "https://doi.org/10.3390/plants11243587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
MDPI., 11(24).
https://doi.org/https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24).
doi:https://doi.org/10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022),
https://doi.org/https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Milanovic, Slobodan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Calic, Dusica D
AU  - Jankovsky, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2376
AB  - Botanicals, such as essential oils (EO) and their compounds, are considered a viable eco-friendly alternative to synthetic insecticides, which threaten human health and ecosystem functioning. In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid) and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology were assessed at different compound concentrations and compared with the effects of the commercial botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results suggest that anethole and carvone might be used as control agents against GML.
PB  - MDPI
T2  - Agronomy 2022, 12(12), 3049
T1  - Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)
VL  - 12
DO  - 10.3390/agronomy12123049
ER  - 

@article{
author = "Kostić, Igor and Milanovic, Slobodan and Kostic, Miroslav and Šešlija Jovanović, Darka and Calic, Dusica D and Jankovsky, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Botanicals, such as essential oils (EO) and their compounds, are considered a viable eco-friendly alternative to synthetic insecticides, which threaten human health and ecosystem functioning. In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid) and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology were assessed at different compound concentrations and compared with the effects of the commercial botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results suggest that anethole and carvone might be used as control agents against GML.",
publisher = "MDPI",
journal = "Agronomy 2022, 12(12), 3049",
title = "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)",
volume = "12",
doi = "10.3390/agronomy12123049"
}

Kostić, I., Milanovic, S., Kostic, M., Šešlija Jovanović, D., Calic, D. D., Jankovsky, L.,& Lazarević, J.. (2022). Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy 2022, 12(12), 3049
MDPI., 12.
https://doi.org/10.3390/agronomy12123049

Kostić I, Milanovic S, Kostic M, Šešlija Jovanović D, Calic DD, Jankovsky L, Lazarević J. Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy 2022, 12(12), 3049. 2022;12.
doi:10.3390/agronomy12123049 .

Kostić, Igor, Milanovic, Slobodan, Kostic, Miroslav, Šešlija Jovanović, Darka, Calic, Dusica D, Jankovsky, Libor, Lazarević, Jelica, "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)" in Agronomy 2022, 12(12), 3049, 12 (2022),
https://doi.org/10.3390/agronomy12123049 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Kostic, Miroslav
AU  - Seslija-Jovanović, Darka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1533
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitasević-Jovanović, Sanja and Kostic, Miroslav and Seslija-Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitasević-Jovanović, S., Kostic, M.,& Seslija-Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitasević-Jovanović S, Kostic M, Seslija-Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitasević-Jovanović, Sanja, Kostic, Miroslav, Seslija-Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022),
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Seslija-Jovanović, Darka
AU  - Kostic, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1404
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Seslija-Jovanović, Darka and Kostic, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194"
}

Kostić, I., Lazarević, J., Seslija-Jovanović, D., Kostic, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel
MDPI, Basel., 10(10).
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Seslija-Jovanović D, Kostic M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel. 2021;10(10).
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Seslija-Jovanović, Darka, Kostic, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants-Basel, 10, no. 10 (2021),
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Seslija-Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Calic, Dusica D
AU  - Stanković, Sladan
AU  - Kostic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1506
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
EP  - 199
IS  - 2
SP  - 190
VL  - 111
DO  - 10.1017/S0007485320000504
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Seslija-Jovanović, Darka and Krnjajić, Slobodan and Calic, Dusica D and Stanković, Sladan and Kostic, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
pages = "199-190",
number = "2",
volume = "111",
doi = "10.1017/S0007485320000504"
}

Lazarević, J., Kostić, I., Milanović, S., Seslija-Jovanović, D., Krnjajić, S., Calic, D. D., Stanković, S.,& Kostic, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 111(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Seslija-Jovanović D, Krnjajić S, Calic DD, Stanković S, Kostic M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;111(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Seslija-Jovanović, Darka, Krnjajić, Slobodan, Calic, Dusica D, Stanković, Sladan, Kostic, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 111, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - CHAP
AU  - Stankovic, Sladjan
AU  - Kostić, MIroslav
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2425
AB  - Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.
PB  - IntechOpen
T2  - In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
T1  - Practical Approaches to Pest Control: The Use of Natural Compounds
EP  - 18
SP  - 1
DO  - 10.5772/intechopen.91792
ER  - 

@inbook{
author = "Stankovic, Sladjan and Kostić, MIroslav and Kostić, Igor and Krnjajić, Slobodan",
year = "2020",
abstract = "Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.",
publisher = "IntechOpen",
journal = "In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen",
booktitle = "Practical Approaches to Pest Control: The Use of Natural Compounds",
pages = "18-1",
doi = "10.5772/intechopen.91792"
}

Stankovic, S., Kostić, M., Kostić, I.,& Krnjajić, S.. (2020). Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
IntechOpen., 1-18.
https://doi.org/10.5772/intechopen.91792

Stankovic S, Kostić M, Kostić I, Krnjajić S. Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen. 2020;:1-18.
doi:10.5772/intechopen.91792 .

Stankovic, Sladjan, Kostić, MIroslav, Kostić, Igor, Krnjajić, Slobodan, "Practical Approaches to Pest Control: The Use of Natural Compounds" in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen (2020):1-18,
https://doi.org/10.5772/intechopen.91792 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Seslija-Jovanović, Darka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1320
AB  - Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - MDPI, Basel
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostic, Miroslav and Vuleta, Ana and Manitasević-Jovanović, Sanja and Seslija-Jovanović, Darka",
year = "2020",
abstract = "Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "MDPI, Basel",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostic, M., Vuleta, A., Manitasević-Jovanović, S.,& Seslija-Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
MDPI, Basel., 11(9).
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostic M, Vuleta A, Manitasević-Jovanović S, Seslija-Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9).
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostic, Miroslav, Vuleta, Ana, Manitasević-Jovanović, Sanja, Seslija-Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020),
https://doi.org/10.3390/insects11090563 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Popović, Marija M.
AU  - Dobrosavljević, Jovan N.
AU  - Kostić, Igor
AU  - Lazarević, Jelica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1379
AB  - Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress
EP  - 69
IS  - 1
SP  - 63
VL  - 72
DO  - 10.2298/ABS191106067M
ER  - 

@article{
author = "Milanović, Slobodan and Popović, Marija M. and Dobrosavljević, Jovan N. and Kostić, Igor and Lazarević, Jelica",
year = "2020",
abstract = "Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress",
pages = "69-63",
number = "1",
volume = "72",
doi = "10.2298/ABS191106067M"
}

Milanović, S., Popović, M. M., Dobrosavljević, J. N., Kostić, I.,& Lazarević, J.. (2020). Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(1), 63-69.
https://doi.org/10.2298/ABS191106067M

Milanović S, Popović MM, Dobrosavljević JN, Kostić I, Lazarević J. Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences. 2020;72(1):63-69.
doi:10.2298/ABS191106067M .

Milanović, Slobodan, Popović, Marija M., Dobrosavljević, Jovan N., Kostić, Igor, Lazarević, Jelica, "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress" in Archives of Biological Sciences, 72, no. 1 (2020):63-69,
https://doi.org/10.2298/ABS191106067M . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Calic, Dusica D
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1343
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control
EP  - 11967
IS  - 11
SP  - 11958
VL  - 27
DO  - 10.1007/s11356-020-07825-1
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Calic, Dusica D",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control",
pages = "11967-11958",
number = "11",
volume = "27",
doi = "10.1007/s11356-020-07825-1"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Calic, D. D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(11), 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Calic DD. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research. 2020;27(11):11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Calic, Dusica D, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control" in Environmental Science and Pollution Research, 27, no. 11 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostic, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjic, Anda
AU  - Kostić, Igor
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1252
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans
EP  - 676
IS  - 4
SP  - 665
VL  - 71
DO  - 10.2298/ABS190617049J
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostic, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjic, Anda and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans",
pages = "676-665",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J"
}

Jevremović, S., Lazarević, J., Kostic, M., Krnjajić, S., Ugrenović, V., Radonjic, A.,& Kostić, I.. (2019). Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostic M, Krnjajić S, Ugrenović V, Radonjic A, Kostić I. Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostic, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjic, Anda, Kostić, Igor, "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - CONF
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Savić, Jelena
AU  - Belic, Maja
AU  - Ćosić, Tatjana
AU  - Calic, Dusica D
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2458
AB  - Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2458
ER  - 

@conference{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Kostić, Miroslav and Savić, Jelena and Belic, Maja and Ćosić, Tatjana and Calic, Dusica D",
year = "2018",
abstract = "Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2458"
}

Devrnja, N., Kostić, I., Lazarević, J., Kostić, M., Savić, J., Belic, M., Ćosić, T.,& Calic, D. D.. (2018). Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society..
https://hdl.handle.net/21.15107/rcub_rimsi_2458

Devrnja N, Kostić I, Lazarević J, Kostić M, Savić J, Belic M, Ćosić T, Calic DD. Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2458 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Kostić, Miroslav, Savić, Jelena, Belic, Maja, Ćosić, Tatjana, Calic, Dusica D, "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2458 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Radojković, Aleksandar
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
AU  - Mitrović, Jelena
AU  - Kostic, Miroslav
AU  - Novaković, Tatjana
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1170
AB  - For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (alpha-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8  lt  F/O-0.5  lt  F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Stored Products Research
T1  - Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)
EP  - 54
SP  - 45
VL  - 77
DO  - 10.1016/j.jspr.2018.02.006
ER  - 

@article{
author = "Lazarević, Jelica and Radojković, Aleksandar and Kostić, Igor and Krnjajić, Slobodan and Mitrović, Jelena and Kostic, Miroslav and Novaković, Tatjana and Branković, Zorica and Branković, Goran",
year = "2018",
abstract = "For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (alpha-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8  lt  F/O-0.5  lt  F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Stored Products Research",
title = "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)",
pages = "54-45",
volume = "77",
doi = "10.1016/j.jspr.2018.02.006"
}

Lazarević, J., Radojković, A., Kostić, I., Krnjajić, S., Mitrović, J., Kostic, M., Novaković, T., Branković, Z.,& Branković, G.. (2018). Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research
Pergamon-Elsevier Science Ltd, Oxford., 77, 45-54.
https://doi.org/10.1016/j.jspr.2018.02.006

Lazarević J, Radojković A, Kostić I, Krnjajić S, Mitrović J, Kostic M, Novaković T, Branković Z, Branković G. Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2018;77:45-54.
doi:10.1016/j.jspr.2018.02.006 .

Lazarević, Jelica, Radojković, Aleksandar, Kostić, Igor, Krnjajić, Slobodan, Mitrović, Jelena, Kostic, Miroslav, Novaković, Tatjana, Branković, Zorica, Branković, Goran, "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 77 (2018):45-54,
https://doi.org/10.1016/j.jspr.2018.02.006 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Milićević, Zoran
AU  - Oro, Violeta
AU  - Kostić, Igor
AU  - Radović, Vesela
AU  - Jelušić, Aleksandra
AU  - Krnjajić, Slobodan
PY  - 2018
UR  - https://plantarum.izbis.bg.ac.rs/handle/123456789/544
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1591
AB  - Numerous scientific research studies all over the world have addressed the problem of
agriculture in the 21st century as being particularly sensitive to climate change, which has caused
phytopathogenic bacteria to spread. Therefore, there is a clear and urgent need to contain this
kind of risk in agricultural production (both conventional and organic farming). The objective
of this study was to determine the antibacterial activity of 30 essential oils (EOs) against three
harmful plant pathogenic bacteria of agricultural importance, Erwinia amylovora, Xanthomonas
campestris pv. campestris and Pseudomonas syringae pv. syringae. The study included in vitro
testing, using an agar-diffusion assay. The EOs of Ceylon cinnamon (leaf and bark), oregano,
clove bud and palmarosa revealed antibacterial activity against the test bacteria, and the
maximum mean inhibition zone diameters of 35 mm was found against E. amylovora and X.
campestris pv. campestris (highly sensitive reaction), while it was smaller in the case of P. syringae
pv. syringae, from 18.25-26.25 mm (sensitive to very sensitive reaction). Maximum diameter of
the zone of inhibition (35 mm) was obtained using basil and peppermint against E. amylovora,
and rosemary, blue gum and camphor tree against X. campestris pv. campestris. Not a single
EO inhibited P. syringae pv. syringae with the resulting total diameter zone of 35 mm, and this
test bacteria was resultingly classified as the least susceptible bacterium of the three tested.
EOs of lemongrass, aniseed, ylang ylang, silver fir, lemon, dwarf mountain pine, bay laurel and
scots pine caused sensitive reaction of the tested bacteria. Peppermint, black cumin, Indian
frankincense, bergamot orange, common juniper, bitter orange and neem produced variable
reactions from total to weakly or no inhibition at all. Weakly activity was found in niaouli and
Atlas cedar. Eastern red cedar, patchouli, Indian sandalwood and ginger caused no reaction of
any of the test bacteria. The results offer a basis for further work based on in vivo testing for the
purpose of developing “natural pesticides” for control of phytopathogenic bacteria, thus giving
a significant contribution to reducing yield losses in agriculture and sustainable development
PB  - Institute of Pesticides and Environmental Protection
T2  - Pesticidi i fitomedicina
T1  - A preliminary study of antibacterial activity of thirty essential oils against several important plant pathogenic bacteria
EP  - 195
IS  - 3-4
SP  - 185
VL  - 33
DO  - 10.2298/PIF1804185P
ER  - 

@article{
author = "Popović, Tatjana and Milićević, Zoran and Oro, Violeta and Kostić, Igor and Radović, Vesela and Jelušić, Aleksandra and Krnjajić, Slobodan",
year = "2018",
abstract = "Numerous scientific research studies all over the world have addressed the problem of
agriculture in the 21st century as being particularly sensitive to climate change, which has caused
phytopathogenic bacteria to spread. Therefore, there is a clear and urgent need to contain this
kind of risk in agricultural production (both conventional and organic farming). The objective
of this study was to determine the antibacterial activity of 30 essential oils (EOs) against three
harmful plant pathogenic bacteria of agricultural importance, Erwinia amylovora, Xanthomonas
campestris pv. campestris and Pseudomonas syringae pv. syringae. The study included in vitro
testing, using an agar-diffusion assay. The EOs of Ceylon cinnamon (leaf and bark), oregano,
clove bud and palmarosa revealed antibacterial activity against the test bacteria, and the
maximum mean inhibition zone diameters of 35 mm was found against E. amylovora and X.
campestris pv. campestris (highly sensitive reaction), while it was smaller in the case of P. syringae
pv. syringae, from 18.25-26.25 mm (sensitive to very sensitive reaction). Maximum diameter of
the zone of inhibition (35 mm) was obtained using basil and peppermint against E. amylovora,
and rosemary, blue gum and camphor tree against X. campestris pv. campestris. Not a single
EO inhibited P. syringae pv. syringae with the resulting total diameter zone of 35 mm, and this
test bacteria was resultingly classified as the least susceptible bacterium of the three tested.
EOs of lemongrass, aniseed, ylang ylang, silver fir, lemon, dwarf mountain pine, bay laurel and
scots pine caused sensitive reaction of the tested bacteria. Peppermint, black cumin, Indian
frankincense, bergamot orange, common juniper, bitter orange and neem produced variable
reactions from total to weakly or no inhibition at all. Weakly activity was found in niaouli and
Atlas cedar. Eastern red cedar, patchouli, Indian sandalwood and ginger caused no reaction of
any of the test bacteria. The results offer a basis for further work based on in vivo testing for the
purpose of developing “natural pesticides” for control of phytopathogenic bacteria, thus giving
a significant contribution to reducing yield losses in agriculture and sustainable development",
publisher = "Institute of Pesticides and Environmental Protection",
journal = "Pesticidi i fitomedicina",
title = "A preliminary study of antibacterial activity of thirty essential oils against several important plant pathogenic bacteria",
pages = "195-185",
number = "3-4",
volume = "33",
doi = "10.2298/PIF1804185P"
}

Popović, T., Milićević, Z., Oro, V., Kostić, I., Radović, V., Jelušić, A.,& Krnjajić, S.. (2018). A preliminary study of antibacterial activity of thirty essential oils against several important plant pathogenic bacteria. in Pesticidi i fitomedicina
Institute of Pesticides and Environmental Protection., 33(3-4), 185-195.
https://doi.org/10.2298/PIF1804185P

Popović T, Milićević Z, Oro V, Kostić I, Radović V, Jelušić A, Krnjajić S. A preliminary study of antibacterial activity of thirty essential oils against several important plant pathogenic bacteria. in Pesticidi i fitomedicina. 2018;33(3-4):185-195.
doi:10.2298/PIF1804185P .

Popović, Tatjana, Milićević, Zoran, Oro, Violeta, Kostić, Igor, Radović, Vesela, Jelušić, Aleksandra, Krnjajić, Slobodan, "A preliminary study of antibacterial activity of thirty essential oils against several important plant pathogenic bacteria" in Pesticidi i fitomedicina, 33, no. 3-4 (2018):185-195,
https://doi.org/10.2298/PIF1804185P . .

TY  - CONF
AU  - Popović, Tatjana
AU  - Kostić, Igor
AU  - Milićević, Zoran
AU  - Gasic, Katarina
AU  - Kostić, Miroslav
AU  - Dervisevic, Marina
AU  - Krnjajić, Slobodan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2747
AB  - Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.
PB  - East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska
C3  - Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
T1  - Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum
EP  - 1383
SP  - 1377
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2747
ER  - 

@conference{
author = "Popović, Tatjana and Kostić, Igor and Milićević, Zoran and Gasic, Katarina and Kostić, Miroslav and Dervisevic, Marina and Krnjajić, Slobodan",
year = "2017",
abstract = "Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.",
publisher = "East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska",
journal = "Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina",
title = "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum",
pages = "1383-1377",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2747"
}

Popović, T., Kostić, I., Milićević, Z., Gasic, K., Kostić, M., Dervisevic, M.,& Krnjajić, S.. (2017). Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska., 8, 1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747

Popović T, Kostić I, Milićević Z, Gasic K, Kostić M, Dervisevic M, Krnjajić S. Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina. 2017;8:1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .

Popović, Tatjana, Kostić, Igor, Milićević, Zoran, Gasic, Katarina, Kostić, Miroslav, Dervisevic, Marina, Krnjajić, Slobodan, "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum" in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina, 8 (2017):1377-1383,
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .

TY  - JOUR
AU  - Toscano, Bruno
AU  - Štrbac, Pero
AU  - Popović, Zorica
AU  - Kostić, Miroslav
AU  - Kostić, Igor
AU  - Konjević, Aleksandra
AU  - Krnjajić, Slobodan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2448
AB  - A faunistic study of the family Elateridae was carried out in Bačka, the north-western
district of Vojvodina Province in Serbia, focusing on their preferred habitats and soil types.
The survey included four locations with a total of 1059 ha, in which 35 species, belonging
to 15 genera of Elateridae, were observed. The frequency of larval and adult forms was
58.94% and 67.40%, respectively, proving that wireworms are regular residents of the area.
Regarding habitat preferences, it was shown that a majority of Elateridae species prefer open
biotopes and wheat crop, while marsh soil, chernozem and alluvium were the preferred soil
types of wireworms.
PB  - Pesticidi i fitomedicina
T2  - Pesticidi i fitomedicina
T1  - A faunistic study of the family Elateridae in Bačka, Serbia
VL  - 32
DO  - 10.2298/PIF1704181T
ER  - 

@article{
author = "Toscano, Bruno and Štrbac, Pero and Popović, Zorica and Kostić, Miroslav and Kostić, Igor and Konjević, Aleksandra and Krnjajić, Slobodan",
year = "2017",
abstract = "A faunistic study of the family Elateridae was carried out in Bačka, the north-western
district of Vojvodina Province in Serbia, focusing on their preferred habitats and soil types.
The survey included four locations with a total of 1059 ha, in which 35 species, belonging
to 15 genera of Elateridae, were observed. The frequency of larval and adult forms was
58.94% and 67.40%, respectively, proving that wireworms are regular residents of the area.
Regarding habitat preferences, it was shown that a majority of Elateridae species prefer open
biotopes and wheat crop, while marsh soil, chernozem and alluvium were the preferred soil
types of wireworms.",
publisher = "Pesticidi i fitomedicina",
journal = "Pesticidi i fitomedicina",
title = "A faunistic study of the family Elateridae in Bačka, Serbia",
volume = "32",
doi = "10.2298/PIF1704181T"
}

Toscano, B., Štrbac, P., Popović, Z., Kostić, M., Kostić, I., Konjević, A.,& Krnjajić, S.. (2017). A faunistic study of the family Elateridae in Bačka, Serbia. in Pesticidi i fitomedicina
Pesticidi i fitomedicina., 32.
https://doi.org/10.2298/PIF1704181T

Toscano B, Štrbac P, Popović Z, Kostić M, Kostić I, Konjević A, Krnjajić S. A faunistic study of the family Elateridae in Bačka, Serbia. in Pesticidi i fitomedicina. 2017;32.
doi:10.2298/PIF1704181T .

Toscano, Bruno, Štrbac, Pero, Popović, Zorica, Kostić, Miroslav, Kostić, Igor, Konjević, Aleksandra, Krnjajić, Slobodan, "A faunistic study of the family Elateridae in Bačka, Serbia" in Pesticidi i fitomedicina, 32 (2017),
https://doi.org/10.2298/PIF1704181T . .

TY  - JOUR
AU  - Zabel, Anton
AU  - Stanković, Slađan
AU  - Kostic, Miroslav
AU  - Rahović, Dragan
AU  - Tomic, Vedran
AU  - Kostić, Igor
AU  - Alkhammas, Iman Omar
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1025
AB  - The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates
EP  - 12596
IS  - 3
SP  - 12584
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1025
ER  - 

@article{
author = "Zabel, Anton and Stanković, Slađan and Kostic, Miroslav and Rahović, Dragan and Tomic, Vedran and Kostić, Igor and Alkhammas, Iman Omar",
year = "2017",
abstract = "The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates",
pages = "12596-12584",
number = "3",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1025"
}

Zabel, A., Stanković, S., Kostic, M., Rahović, D., Tomic, V., Kostić, I.,& Alkhammas, I. O.. (2017). Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 22(3), 12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025

Zabel A, Stanković S, Kostic M, Rahović D, Tomic V, Kostić I, Alkhammas IO. Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters. 2017;22(3):12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .

Zabel, Anton, Stanković, Slađan, Kostic, Miroslav, Rahović, Dragan, Tomic, Vedran, Kostić, Igor, Alkhammas, Iman Omar, "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates" in Romanian Biotechnological Letters, 22, no. 3 (2017):12584-12596,
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Jankovic-Tomanic, Milena
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/990
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
ER  - 

@article{
author = "Milanović, Slobodan and Jankovic-Tomanic, Milena and Kostić, Igor and Kostic, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388"
}

Milanović, S., Jankovic-Tomanic, M., Kostić, I., Kostic, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata
Wiley, Hoboken., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Jankovic-Tomanic M, Kostić I, Kostic M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Jankovic-Tomanic, Milena, Kostić, Igor, Kostic, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .

TY  - THES
AU  - Kostić, Igor
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4875
UR  - https://nardus.mpn.gov.rs/handle/123456789/8012
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:15283/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48831759
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/15
AB  - U radu je ispitivano delovanje alkoholnih rastvora etarskih ulja anisa, mirođije i morača i njihovih dominantnih komponenata trans-anetola i karvona na gusenice gubara (Lymantria dispar L.). Antifidna aktivnost etarskih ulja i njihovih dominantnih komponenata primenjenih u koncentracijama 0.1, 0.5 i 1.0 % ispitivana je u dva odvojena ogleda, ogledu bez izbora i ogledu sa izborom. Ispitivanje je vršeno na gusenicama drugog stupnja, a rezultati ogleda su očitavani nakon 48 časova od početka ogleda. Rezidualna kontaktna i digestivna toksičnost etarskih ulja anisa, mirođije i morača i njihovih dominantnih komponenata primenjenih u koncentracijama 0.05, 0.1, 0.25, 0.5 i 1.0 %, ispitivana je na gusenicama gubara drugog stupnja. Pored mortaliteta gusenica, praćen je i uticaj navedenih toksičnosti na presvlačenje gusenica iz drugog u treći larveni stupanj. Ogledi u kojima je ispitivan uticaj toksičnosti ispitivanih etarskih ulja i nihovih dominantnih komponenata na mortalitet i zaustavljanje presvlačenja gusenica trajali su 120 časova, a rezultati su očitavani na svaka 24 časa. Delovanje ispitivanih etarskih ulja i njihovih dominantnih komponenata, primenjenih u koncentracijama 0.1, 0.25 i 0.5 %, na indekse rasta i ishrane gusenica gubara četvrtog stupnjsa sagledavano je u ogledima za ispitivanja njihovog uticaja na relativnu brzine rasta gusenica (RGR), relativnu brzinu konzumacije hrane gusenica (RCR), efikasnost asimilacije hrane kod gusenica (AD) i efikasnosti konverzije unete i svarene hrane kod gusenica (ECI i ECD). Rezultati ogleda su očitavani nakon 48 časova od početka ogleda. Za poređenje rezultata je korišćeno biološko sredstvo NeemAzal (standard). Statistička obrada podataka izvršena je uz pomoć softverskog paketa Statistica 7.0. (StatSoft, Inc). Analizom dobijenih rezultata utvrđeno je da dobru antifidnu aktivnost poseduju etarsko ulje anisa i njegova dominantna komponenta trans-anetol primenjeni u koncentraciji 1.0 %. Rezidualna kontaktna toksičnost ispitivanih etarskih ulja i njihovih dominantnih komponata nije konstatovana, a njihov uticaj na zaustavljanje presvlačenja gusenica nije zadovoljavajuć. Visoku digestivnu toksičnost koja dovodi do mortaliteta 100 % gusenica ili 100 % zaustavljanja presvlačenja gusenica, ukoliko nisu sve gusenice uginule, poseduju ispitivana etarska ulja primenjena u koncentraciji 1.0 %, trans-anetol primenjen u koncentracijama 0.5 i 1.0 % i karvon primenjen u koncentracijama 0.25, 0.5 i 1.0 %. Indeksi rasta i ishrane gusenica gubara su primenom ispitivanih etarskih ulja i njihovih dominantnih komponenata značajno sniženi. Primenjena sredstva imaju značajan uticaj na snižavanje relativne brzine rasta gusenica (RGR), relativne brzine konzumacije hrane gusenica (RCR), koeficijent asimilacije hrane kod gusenica (AD) i efikasnosti konverzije unete i svarene hrane u biomasu gusenica (ECI i ECD)...
AB  - In this work the influence of ethanol solutions of essential oils of anise, fennel and dill and their dominant components trans-anethole and carvone on gypsy moth larvae (Lymantria dispar L.) was tested. Antifeedant activity of essential oils and their dominant components applied in concentrantions 0.1, 0.5 i 1.0 % was tested in two separate tests, no choice and choice feeding ones. The testing was done on the second instar and the results of the experiments were recorded 48 h after the beginning of the experiment. Residual concant and digestive toxicity of essential oils of anise, fennel and dill and their dominant components applied in concentrations 0.05, 0.1, 0.25, 0.5 i 1.0 % were tested on the second instar gypsy moth larvae. The influence of mentioned toxicity on moulting from the second to the third instar was followed, too. The influence of toxicity of essential oils and their dominant components on mortality and disturbing moulting was evaluated after 24, 48, 72, 96 and 120 h after the beginning of the experiment. The influence of tested essential oils and their dominant components applied in concentrations 0.1, 0.25, 0.5 % on growth and feeding indices of the fourth insters was tested in the asseys about their influence on relative growth rate (RGR), relative consuption rate (RCR), aproximate digestibility (AD) and efficiency of conversation of ingested food (ECI), as well as efficiency of conversation of digested food (ECD). The results were recorded 48 h after the beginning of the experiment. Botanical standard NeemAzal was used for comparing results. Statistical data proccesing was done by softer Statistica 7.0. The analyzying of the results confirmed that essential oil of anise and its dominant component tras-anethole applied in concentration 1.0 % possess good antifeedant activity. Residual contact toksicity of tested essential oils and their dominant components as well as their influence on disturbing moulting was not satisfied. Tested essential oils applied in concentration 1.0 % possess high digestive toxicity which causes 100 % larvae mortality or 100 % inhibits moulting. Tras-anthpole posses the same effect in concentrations 0.5 and 1.0 % as well as carvone in concentrations 25, 0.5 and 1.0 %. Applied compounds have significant influence on relative growth rate (RGR), relative consuption rate (RCR), aproximate digestibility (AD) and efficiency of conversation of ingested food (ECI), as well as efficiency of conversation of digested food (ECD). They are most effective in concentracion 0.5 % and carvone, in average, has significantly bigger influence in comparison with other applied compounds...
PB  - Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Dejstvo etarskih ulja anisa, morača i mirođije i njihovih dominantnih komponenti na larve gubara (Lymantria dispar L.)
T1  - The effect of essential oils of anise, fennel and dill and their dominant components on gypsy moth larvae (Lymantria dispar L.)
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8012
ER  - 

@phdthesis{
author = "Kostić, Igor",
year = "2016",
abstract = "U radu je ispitivano delovanje alkoholnih rastvora etarskih ulja anisa, mirođije i morača i njihovih dominantnih komponenata trans-anetola i karvona na gusenice gubara (Lymantria dispar L.). Antifidna aktivnost etarskih ulja i njihovih dominantnih komponenata primenjenih u koncentracijama 0.1, 0.5 i 1.0 % ispitivana je u dva odvojena ogleda, ogledu bez izbora i ogledu sa izborom. Ispitivanje je vršeno na gusenicama drugog stupnja, a rezultati ogleda su očitavani nakon 48 časova od početka ogleda. Rezidualna kontaktna i digestivna toksičnost etarskih ulja anisa, mirođije i morača i njihovih dominantnih komponenata primenjenih u koncentracijama 0.05, 0.1, 0.25, 0.5 i 1.0 %, ispitivana je na gusenicama gubara drugog stupnja. Pored mortaliteta gusenica, praćen je i uticaj navedenih toksičnosti na presvlačenje gusenica iz drugog u treći larveni stupanj. Ogledi u kojima je ispitivan uticaj toksičnosti ispitivanih etarskih ulja i nihovih dominantnih komponenata na mortalitet i zaustavljanje presvlačenja gusenica trajali su 120 časova, a rezultati su očitavani na svaka 24 časa. Delovanje ispitivanih etarskih ulja i njihovih dominantnih komponenata, primenjenih u koncentracijama 0.1, 0.25 i 0.5 %, na indekse rasta i ishrane gusenica gubara četvrtog stupnjsa sagledavano je u ogledima za ispitivanja njihovog uticaja na relativnu brzine rasta gusenica (RGR), relativnu brzinu konzumacije hrane gusenica (RCR), efikasnost asimilacije hrane kod gusenica (AD) i efikasnosti konverzije unete i svarene hrane kod gusenica (ECI i ECD). Rezultati ogleda su očitavani nakon 48 časova od početka ogleda. Za poređenje rezultata je korišćeno biološko sredstvo NeemAzal (standard). Statistička obrada podataka izvršena je uz pomoć softverskog paketa Statistica 7.0. (StatSoft, Inc). Analizom dobijenih rezultata utvrđeno je da dobru antifidnu aktivnost poseduju etarsko ulje anisa i njegova dominantna komponenta trans-anetol primenjeni u koncentraciji 1.0 %. Rezidualna kontaktna toksičnost ispitivanih etarskih ulja i njihovih dominantnih komponata nije konstatovana, a njihov uticaj na zaustavljanje presvlačenja gusenica nije zadovoljavajuć. Visoku digestivnu toksičnost koja dovodi do mortaliteta 100 % gusenica ili 100 % zaustavljanja presvlačenja gusenica, ukoliko nisu sve gusenice uginule, poseduju ispitivana etarska ulja primenjena u koncentraciji 1.0 %, trans-anetol primenjen u koncentracijama 0.5 i 1.0 % i karvon primenjen u koncentracijama 0.25, 0.5 i 1.0 %. Indeksi rasta i ishrane gusenica gubara su primenom ispitivanih etarskih ulja i njihovih dominantnih komponenata značajno sniženi. Primenjena sredstva imaju značajan uticaj na snižavanje relativne brzine rasta gusenica (RGR), relativne brzine konzumacije hrane gusenica (RCR), koeficijent asimilacije hrane kod gusenica (AD) i efikasnosti konverzije unete i svarene hrane u biomasu gusenica (ECI i ECD)..., In this work the influence of ethanol solutions of essential oils of anise, fennel and dill and their dominant components trans-anethole and carvone on gypsy moth larvae (Lymantria dispar L.) was tested. Antifeedant activity of essential oils and their dominant components applied in concentrantions 0.1, 0.5 i 1.0 % was tested in two separate tests, no choice and choice feeding ones. The testing was done on the second instar and the results of the experiments were recorded 48 h after the beginning of the experiment. Residual concant and digestive toxicity of essential oils of anise, fennel and dill and their dominant components applied in concentrations 0.05, 0.1, 0.25, 0.5 i 1.0 % were tested on the second instar gypsy moth larvae. The influence of mentioned toxicity on moulting from the second to the third instar was followed, too. The influence of toxicity of essential oils and their dominant components on mortality and disturbing moulting was evaluated after 24, 48, 72, 96 and 120 h after the beginning of the experiment. The influence of tested essential oils and their dominant components applied in concentrations 0.1, 0.25, 0.5 % on growth and feeding indices of the fourth insters was tested in the asseys about their influence on relative growth rate (RGR), relative consuption rate (RCR), aproximate digestibility (AD) and efficiency of conversation of ingested food (ECI), as well as efficiency of conversation of digested food (ECD). The results were recorded 48 h after the beginning of the experiment. Botanical standard NeemAzal was used for comparing results. Statistical data proccesing was done by softer Statistica 7.0. The analyzying of the results confirmed that essential oil of anise and its dominant component tras-anethole applied in concentration 1.0 % possess good antifeedant activity. Residual contact toksicity of tested essential oils and their dominant components as well as their influence on disturbing moulting was not satisfied. Tested essential oils applied in concentration 1.0 % possess high digestive toxicity which causes 100 % larvae mortality or 100 % inhibits moulting. Tras-anthpole posses the same effect in concentrations 0.5 and 1.0 % as well as carvone in concentrations 25, 0.5 and 1.0 %. Applied compounds have significant influence on relative growth rate (RGR), relative consuption rate (RCR), aproximate digestibility (AD) and efficiency of conversation of ingested food (ECI), as well as efficiency of conversation of digested food (ECD). They are most effective in concentracion 0.5 % and carvone, in average, has significantly bigger influence in comparison with other applied compounds...",
publisher = "Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Dejstvo etarskih ulja anisa, morača i mirođije i njihovih dominantnih komponenti na larve gubara (Lymantria dispar L.), The effect of essential oils of anise, fennel and dill and their dominant components on gypsy moth larvae (Lymantria dispar L.)",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8012"
}

Kostić, I.. (2016). Dejstvo etarskih ulja anisa, morača i mirođije i njihovih dominantnih komponenti na larve gubara (Lymantria dispar L.). 
Univerzitet u Beogradu, Poljoprivredni fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_8012

Kostić I. Dejstvo etarskih ulja anisa, morača i mirođije i njihovih dominantnih komponenti na larve gubara (Lymantria dispar L.). 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_8012 .

Kostić, Igor, "Dejstvo etarskih ulja anisa, morača i mirođije i njihovih dominantnih komponenti na larve gubara (Lymantria dispar L.)" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_8012 .

TY  - CONF
AU  - Krnjajić, Slobodan
AU  - Radojković, Aleksandar
AU  - Radović, Marko
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Mitrović, Jelena
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2858
AB  - Insecticide effect of ZnO, TiO2, AL2O3 and zeolite powders were studied on bean weevil (Achanthoscelides obtectus Say, 1831). Mortality of both male and female adults was significantly higher for zeolite and Al2O3 powders than ZnO and TiO2, while females were less suspectibile than male. SEM analysis of the insects treated with Al2O3 revealet that disability and mortality of bean weevils can be related to degree of powder covergate of the insects exosceleton. All powders showed similar effect to development of F1 progeny with reduction in larvae number over 50%, and can be considered for use in organic production.
PB  - University of Belgrade-Technical Faculty of Bor, Srbija
C3  - Proceedings XXIII International Conference Ecological Truth
T1  - Insecticide effects of non-toxic inorganic powders against bean weevil (Acanthoscelides obtectus)
EP  - 486
SP  - 480
VL  - 13
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2858
ER  - 

@conference{
author = "Krnjajić, Slobodan and Radojković, Aleksandar and Radović, Marko and Kostić, Igor and Kostić, Miroslav and Mitrović, Jelena and Branković, Zorica and Branković, Goran",
year = "2015",
abstract = "Insecticide effect of ZnO, TiO2, AL2O3 and zeolite powders were studied on bean weevil (Achanthoscelides obtectus Say, 1831). Mortality of both male and female adults was significantly higher for zeolite and Al2O3 powders than ZnO and TiO2, while females were less suspectibile than male. SEM analysis of the insects treated with Al2O3 revealet that disability and mortality of bean weevils can be related to degree of powder covergate of the insects exosceleton. All powders showed similar effect to development of F1 progeny with reduction in larvae number over 50%, and can be considered for use in organic production.",
publisher = "University of Belgrade-Technical Faculty of Bor, Srbija",
journal = "Proceedings XXIII International Conference Ecological Truth",
title = "Insecticide effects of non-toxic inorganic powders against bean weevil (Acanthoscelides obtectus)",
pages = "486-480",
volume = "13",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2858"
}

Krnjajić, S., Radojković, A., Radović, M., Kostić, I., Kostić, M., Mitrović, J., Branković, Z.,& Branković, G.. (2015). Insecticide effects of non-toxic inorganic powders against bean weevil (Acanthoscelides obtectus). in Proceedings XXIII International Conference Ecological Truth
University of Belgrade-Technical Faculty of Bor, Srbija., 13, 480-486.
https://hdl.handle.net/21.15107/rcub_rimsi_2858

Krnjajić S, Radojković A, Radović M, Kostić I, Kostić M, Mitrović J, Branković Z, Branković G. Insecticide effects of non-toxic inorganic powders against bean weevil (Acanthoscelides obtectus). in Proceedings XXIII International Conference Ecological Truth. 2015;13:480-486.
https://hdl.handle.net/21.15107/rcub_rimsi_2858 .

Krnjajić, Slobodan, Radojković, Aleksandar, Radović, Marko, Kostić, Igor, Kostić, Miroslav, Mitrović, Jelena, Branković, Zorica, Branković, Goran, "Insecticide effects of non-toxic inorganic powders against bean weevil (Acanthoscelides obtectus)" in Proceedings XXIII International Conference Ecological Truth, 13 (2015):480-486,
https://hdl.handle.net/21.15107/rcub_rimsi_2858 .

TY  - CONF
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
AU  - Radojković, Aleksandar
AU  - Radović, Marko
AU  - Savić, Slavica M.
AU  - Kostić, Miroslav
AU  - Mitrović, Jelena
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2744
AB  - Alumina powders were obtained by auto combustion method and calcined for 1 h at
temperatures ranging from 1000ºC to 1200ºC. X-ray powder diffraction analysis revealed an
overwhelming presence of γ–Al2O3 for the powder calcined at 1000ºC, while at 1200ºC only
the presence of α–Al2O3 was detected. Insecticide properties of these powders were
investigated on bean weevil (Acanthoscelides obtectus Say). Mortality of treated and
untreated male and female adults was checked in a period of 7 days. The powder calcined at
1200ºC was the most efficient, and 100% mortality of both male and female insects were
reached after 7 days of exposure. It was also observed that insecticide efficiency increased
with the increase of α–Al2O3 phase present in the powders and the female insects were less
susceptible to the powders’ toxicity in general. Scanning electron microscope analysis of the
treated adults with Al2O3 indicated that mortality of bean weevils can be related to adsorption
of the powders onto their exoskeleton. Development of F1 progeny was similar for all the
powders and the number of hatched larvae was significantly reduced in comparison with the
untreated seeds, while the percentage of damaged seeds dropped from 70% to 15%. These
results suggest that relatively cheap and non-toxic α–Al2O3 powders can be considered for use
in pest control, especially in organic seed production
PB  - 6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina
C3  - 6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina
T1  - Alumina powders as novel non-toxic insecticide against bean weevil (Acanthoscelides obtectus Say)
EP  - 948
SP  - 943
VL  - 6
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2744
ER  - 

@conference{
author = "Kostić, Igor and Krnjajić, Slobodan and Radojković, Aleksandar and Radović, Marko and Savić, Slavica M. and Kostić, Miroslav and Mitrović, Jelena and Branković, Zorica and Branković, Goran",
year = "2015",
abstract = "Alumina powders were obtained by auto combustion method and calcined for 1 h at
temperatures ranging from 1000ºC to 1200ºC. X-ray powder diffraction analysis revealed an
overwhelming presence of γ–Al2O3 for the powder calcined at 1000ºC, while at 1200ºC only
the presence of α–Al2O3 was detected. Insecticide properties of these powders were
investigated on bean weevil (Acanthoscelides obtectus Say). Mortality of treated and
untreated male and female adults was checked in a period of 7 days. The powder calcined at
1200ºC was the most efficient, and 100% mortality of both male and female insects were
reached after 7 days of exposure. It was also observed that insecticide efficiency increased
with the increase of α–Al2O3 phase present in the powders and the female insects were less
susceptible to the powders’ toxicity in general. Scanning electron microscope analysis of the
treated adults with Al2O3 indicated that mortality of bean weevils can be related to adsorption
of the powders onto their exoskeleton. Development of F1 progeny was similar for all the
powders and the number of hatched larvae was significantly reduced in comparison with the
untreated seeds, while the percentage of damaged seeds dropped from 70% to 15%. These
results suggest that relatively cheap and non-toxic α–Al2O3 powders can be considered for use
in pest control, especially in organic seed production",
publisher = "6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina",
journal = "6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina",
title = "Alumina powders as novel non-toxic insecticide against bean weevil (Acanthoscelides obtectus Say)",
pages = "948-943",
volume = "6",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2744"
}

Kostić, I., Krnjajić, S., Radojković, A., Radović, M., Savić, S. M., Kostić, M., Mitrović, J., Branković, Z.,& Branković, G.. (2015). Alumina powders as novel non-toxic insecticide against bean weevil (Acanthoscelides obtectus Say). in 6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina
6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina., 6, 943-948.
https://hdl.handle.net/21.15107/rcub_rimsi_2744

Kostić I, Krnjajić S, Radojković A, Radović M, Savić SM, Kostić M, Mitrović J, Branković Z, Branković G. Alumina powders as novel non-toxic insecticide against bean weevil (Acanthoscelides obtectus Say). in 6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina. 2015;6:943-948.
https://hdl.handle.net/21.15107/rcub_rimsi_2744 .

Kostić, Igor, Krnjajić, Slobodan, Radojković, Aleksandar, Radović, Marko, Savić, Slavica M., Kostić, Miroslav, Mitrović, Jelena, Branković, Zorica, Branković, Goran, "Alumina powders as novel non-toxic insecticide against bean weevil (Acanthoscelides obtectus Say)" in 6th International Scientific Agricultural Symposium „Agrosym 2015“, Jahorina, 6 (2015):943-948,
https://hdl.handle.net/21.15107/rcub_rimsi_2744 .

TY  - CONF
AU  - Radović, Marko
AU  - Radojković, Aleksandar
AU  - Kostić, Igor
AU  - Mitrović, Jelena
AU  - Krnjajić, Slobodan
AU  - Kostić, Miodrag
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2362
AB  - Alumina powders were synthesized by citrate/nitrate autocombustion method with
different citrate/nitrate molar ratios (0.5, 0.8 and 1.2). XRD analysis of the powders
calcined at 1200 °C for 1 h showed only the presence of α–Al2O3 phase. Particle size of
the powders determined by SEM analysis varied from nano- and submicron size of
several µm. The powders were mixed with grains of bean to examine their insecticide
effect against bean weevil (Acanthoscelides obtectus Say, 1831). Mortality of insects
after seven days of exposure was the highest for the powder synthesized at 1.2 molar
ratio, which contained the highest portion of submicron particles. This suggested that
lager amount of citric acid could prevent higher degree of particles agglomeration.
Mortality cause of insects is assumed to be related to the degree of powder coverage of
the insects’ exoskeleton. The same powder also provided the best efficiency in
suppressing the emerge of F1 generation and seed protection even at lower
concentrations.
PB  - Univerzitet u Novom Sadu, Tehnološki fakultet
C3  - 11th Conference for Young Scientists in Ceramics (SM- 2015)
T1  - Synthesis of Alumina Powders and their Insecticidal Effect Against Acanthoscelides Obtectus Say
SP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2362
ER  - 

@conference{
author = "Radović, Marko and Radojković, Aleksandar and Kostić, Igor and Mitrović, Jelena and Krnjajić, Slobodan and Kostić, Miodrag and Branković, Zorica and Branković, Goran",
year = "2015",
abstract = "Alumina powders were synthesized by citrate/nitrate autocombustion method with
different citrate/nitrate molar ratios (0.5, 0.8 and 1.2). XRD analysis of the powders
calcined at 1200 °C for 1 h showed only the presence of α–Al2O3 phase. Particle size of
the powders determined by SEM analysis varied from nano- and submicron size of
several µm. The powders were mixed with grains of bean to examine their insecticide
effect against bean weevil (Acanthoscelides obtectus Say, 1831). Mortality of insects
after seven days of exposure was the highest for the powder synthesized at 1.2 molar
ratio, which contained the highest portion of submicron particles. This suggested that
lager amount of citric acid could prevent higher degree of particles agglomeration.
Mortality cause of insects is assumed to be related to the degree of powder coverage of
the insects’ exoskeleton. The same powder also provided the best efficiency in
suppressing the emerge of F1 generation and seed protection even at lower
concentrations.",
publisher = "Univerzitet u Novom Sadu, Tehnološki fakultet",
journal = "11th Conference for Young Scientists in Ceramics (SM- 2015)",
title = "Synthesis of Alumina Powders and their Insecticidal Effect Against Acanthoscelides Obtectus Say",
pages = "54",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2362"
}

Radović, M., Radojković, A., Kostić, I., Mitrović, J., Krnjajić, S., Kostić, M., Branković, Z.,& Branković, G.. (2015). Synthesis of Alumina Powders and their Insecticidal Effect Against Acanthoscelides Obtectus Say. in 11th Conference for Young Scientists in Ceramics (SM- 2015)
Univerzitet u Novom Sadu, Tehnološki fakultet., 54.
https://hdl.handle.net/21.15107/rcub_rimsi_2362

Radović M, Radojković A, Kostić I, Mitrović J, Krnjajić S, Kostić M, Branković Z, Branković G. Synthesis of Alumina Powders and their Insecticidal Effect Against Acanthoscelides Obtectus Say. in 11th Conference for Young Scientists in Ceramics (SM- 2015). 2015;:54.
https://hdl.handle.net/21.15107/rcub_rimsi_2362 .

Radović, Marko, Radojković, Aleksandar, Kostić, Igor, Mitrović, Jelena, Krnjajić, Slobodan, Kostić, Miodrag, Branković, Zorica, Branković, Goran, "Synthesis of Alumina Powders and their Insecticidal Effect Against Acanthoscelides Obtectus Say" in 11th Conference for Young Scientists in Ceramics (SM- 2015) (2015):54,
https://hdl.handle.net/21.15107/rcub_rimsi_2362 .

TY  - CONF
AU  - Radojković, Aleksandar
AU  - Radović, Marko
AU  - Krnjajić, Slobodan
AU  - Kostić, Igor
AU  - Mitrović, Jelena
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2336
AB  - Alumina powders synthesized by combustion method were applied on
uninfested grains of bean to examine their insecticide effect against bean weevil
(Acanthoscelides obtectus). Effects of pH value (6, 8 and 10) of the precursor
solution, as well as citric/nitric molar ratio (0.5, 0.8 and 1.2) on the powder
properties were observed. XRD analysis of the samples calcined at 1200 ºC for 1 h
showed only the presence of α–Al2O3 phase. SEM analysis revealed difference
between the samples in respect to particle size, which varied from nano- and
submicron size to characteristic rectangular particles of several µm and larger. The
powder obtained from the solution with the pH value of 10 had the highest content
of submicron and nanosized particles. Mortality rate of both male and female insects
after seven days of exposure was the highest right for this sample, indicating that the
particle size has the greatest influence on insecticide properties of alumina powders.
Thus, by increasing the pH of the precursor solution it is possible to prevent higher
degree of particles agglomeration. The lowest insecticide efficiency of the powder
synthesized at pH = 6 can be prescribed to a weak chelating effect of the citric acid
that allowed higher coarsening rate of the particles. When it comes to citric/nitric
molar ratio at pH = 8, it had no significant effect on mortality rates, which were
higher for male insects in general, while a slight efficiency trend was observed
towards fuel lean region, i.e. when the ratio was ≤ 0.8.
PB  - Društvo za keramičke materijale Srbije
C3  - 3rd Conference of the Serbian Society for Ceramic Materials
T1  - INSECTICIDE EFFECT OF ALUMINA POWDERS AGAINST - BEAN WEEVILS
SP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2336
ER  - 

@conference{
author = "Radojković, Aleksandar and Radović, Marko and Krnjajić, Slobodan and Kostić, Igor and Mitrović, Jelena and Branković, Zorica and Branković, Goran",
year = "2015",
abstract = "Alumina powders synthesized by combustion method were applied on
uninfested grains of bean to examine their insecticide effect against bean weevil
(Acanthoscelides obtectus). Effects of pH value (6, 8 and 10) of the precursor
solution, as well as citric/nitric molar ratio (0.5, 0.8 and 1.2) on the powder
properties were observed. XRD analysis of the samples calcined at 1200 ºC for 1 h
showed only the presence of α–Al2O3 phase. SEM analysis revealed difference
between the samples in respect to particle size, which varied from nano- and
submicron size to characteristic rectangular particles of several µm and larger. The
powder obtained from the solution with the pH value of 10 had the highest content
of submicron and nanosized particles. Mortality rate of both male and female insects
after seven days of exposure was the highest right for this sample, indicating that the
particle size has the greatest influence on insecticide properties of alumina powders.
Thus, by increasing the pH of the precursor solution it is possible to prevent higher
degree of particles agglomeration. The lowest insecticide efficiency of the powder
synthesized at pH = 6 can be prescribed to a weak chelating effect of the citric acid
that allowed higher coarsening rate of the particles. When it comes to citric/nitric
molar ratio at pH = 8, it had no significant effect on mortality rates, which were
higher for male insects in general, while a slight efficiency trend was observed
towards fuel lean region, i.e. when the ratio was ≤ 0.8.",
publisher = "Društvo za keramičke materijale Srbije",
journal = "3rd Conference of the Serbian Society for Ceramic Materials",
title = "INSECTICIDE EFFECT OF ALUMINA POWDERS AGAINST - BEAN WEEVILS",
pages = "76",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2336"
}

Radojković, A., Radović, M., Krnjajić, S., Kostić, I., Mitrović, J., Branković, Z.,& Branković, G.. (2015). INSECTICIDE EFFECT OF ALUMINA POWDERS AGAINST - BEAN WEEVILS. in 3rd Conference of the Serbian Society for Ceramic Materials
Društvo za keramičke materijale Srbije., 76.
https://hdl.handle.net/21.15107/rcub_rimsi_2336

Radojković A, Radović M, Krnjajić S, Kostić I, Mitrović J, Branković Z, Branković G. INSECTICIDE EFFECT OF ALUMINA POWDERS AGAINST - BEAN WEEVILS. in 3rd Conference of the Serbian Society for Ceramic Materials. 2015;:76.
https://hdl.handle.net/21.15107/rcub_rimsi_2336 .

Radojković, Aleksandar, Radović, Marko, Krnjajić, Slobodan, Kostić, Igor, Mitrović, Jelena, Branković, Zorica, Branković, Goran, "INSECTICIDE EFFECT OF ALUMINA POWDERS AGAINST - BEAN WEEVILS" in 3rd Conference of the Serbian Society for Ceramic Materials (2015):76,
https://hdl.handle.net/21.15107/rcub_rimsi_2336 .