TY  - CONF
AU  - Popović, Tatjana
AU  - Kostić, Igor
AU  - Milićević, Zoran
AU  - Gasic, Katarina
AU  - Kostić, Miroslav
AU  - Dervisevic, Marina
AU  - Krnjajić, Slobodan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2747
AB  - Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.
PB  - East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska
C3  - Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
T1  - Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum
EP  - 1383
SP  - 1377
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2747
ER  - 

@conference{
author = "Popović, Tatjana and Kostić, Igor and Milićević, Zoran and Gasic, Katarina and Kostić, Miroslav and Dervisevic, Marina and Krnjajić, Slobodan",
year = "2017",
abstract = "Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.",
publisher = "East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska",
journal = "Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina",
title = "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum",
pages = "1383-1377",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2747"
}

Popović, T., Kostić, I., Milićević, Z., Gasic, K., Kostić, M., Dervisevic, M.,& Krnjajić, S.. (2017). Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska., 8, 1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747

Popović T, Kostić I, Milićević Z, Gasic K, Kostić M, Dervisevic M, Krnjajić S. Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina. 2017;8:1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .

Popović, Tatjana, Kostić, Igor, Milićević, Zoran, Gasic, Katarina, Kostić, Miroslav, Dervisevic, Marina, Krnjajić, Slobodan, "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum" in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina, 8 (2017):1377-1383,
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .

TY  - CONF
AU  - Kostić, Miroslav
AU  - Kostić, Igor
AU  - Marković, Tatjana
AU  - Jevđović, Radosav
AU  - Stanković, Slađan
AU  - Todorović, Goran
AU  - Nedić, Nebojša
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2803
AB  - Colorado potato beetle has a very high reproductive potential in Serbia due to favorable
climatic conditions and nutrition. Considering that it is an introduced species, the activity of
limiting biotic factors seems negligible. With the time, it developed resistance on commercial
insecticides, and consequently they cease to be used for this purpose. In recent years, not only
in the EU, a strong public pressure has been posed on production of safe food, i.e. food with
no pesticide residues which are harmful and dangerous to human health. For these reasons,
today we examine several alternative routes, such as: the creation of resistant potato varieties
(transgenic potatoes), joint cultivation of potatoes with plants with efficacy to repel or
confuse the potato beetle. A great number of herbal extracts and essential oil has been studied
so far in order to find natural solution that will replace conventional insecticides. In this
study, the possibility to disrupt the attractant properties of potato leaf on Colorado potato
beetles, by application of 95% ethanol solutions of sage essential oil and its fractions (F1-F5)
(0.5% concentration) was examined. Chemical composition of sage essential oil and its five
fractions used in experiment was presented. Tests were conducted at the Adult (female)
Leptinotarsa decemlineata Say in the olfactometer. The experiments were set up in five
replicates, in micro-climatic chamber, with following constant conditions: temperature 27°C
± 1°C, relative humidity 65% ± 5% and the illumination was 9400p cd. Results were
processed by the use of Analysis of variance and Duncan’s test. The most pronounced
disturbance was recorded with application of sage essential oil and the least one was achieved
with application of the fraction one (F1). The obtained results indicate possibility of using
secondary metabolites of Salvia officinalis L. in protection of potato foliage of its major pest
(L. decemlineata).
PB  - Association for Medicinal and Aromatic Plants of Southeast European Countries (AMAPSEEC) & Institute for Medicinal Plant Research “Dr Josif Pančić”, Belgrade, Srbija
C3  - Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries
T1  - Disruption of attractant properties of potato foliage on Leptinotarsa decemlineata Say by the use of Salvia officinalis L. essential oil
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2803
ER  - 

@conference{
author = "Kostić, Miroslav and Kostić, Igor and Marković, Tatjana and Jevđović, Radosav and Stanković, Slađan and Todorović, Goran and Nedić, Nebojša",
year = "2012",
abstract = "Colorado potato beetle has a very high reproductive potential in Serbia due to favorable
climatic conditions and nutrition. Considering that it is an introduced species, the activity of
limiting biotic factors seems negligible. With the time, it developed resistance on commercial
insecticides, and consequently they cease to be used for this purpose. In recent years, not only
in the EU, a strong public pressure has been posed on production of safe food, i.e. food with
no pesticide residues which are harmful and dangerous to human health. For these reasons,
today we examine several alternative routes, such as: the creation of resistant potato varieties
(transgenic potatoes), joint cultivation of potatoes with plants with efficacy to repel or
confuse the potato beetle. A great number of herbal extracts and essential oil has been studied
so far in order to find natural solution that will replace conventional insecticides. In this
study, the possibility to disrupt the attractant properties of potato leaf on Colorado potato
beetles, by application of 95% ethanol solutions of sage essential oil and its fractions (F1-F5)
(0.5% concentration) was examined. Chemical composition of sage essential oil and its five
fractions used in experiment was presented. Tests were conducted at the Adult (female)
Leptinotarsa decemlineata Say in the olfactometer. The experiments were set up in five
replicates, in micro-climatic chamber, with following constant conditions: temperature 27°C
± 1°C, relative humidity 65% ± 5% and the illumination was 9400p cd. Results were
processed by the use of Analysis of variance and Duncan’s test. The most pronounced
disturbance was recorded with application of sage essential oil and the least one was achieved
with application of the fraction one (F1). The obtained results indicate possibility of using
secondary metabolites of Salvia officinalis L. in protection of potato foliage of its major pest
(L. decemlineata).",
publisher = "Association for Medicinal and Aromatic Plants of Southeast European Countries (AMAPSEEC) & Institute for Medicinal Plant Research “Dr Josif Pančić”, Belgrade, Srbija",
journal = "Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries",
title = "Disruption of attractant properties of potato foliage on Leptinotarsa decemlineata Say by the use of Salvia officinalis L. essential oil",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2803"
}

Kostić, M., Kostić, I., Marković, T., Jevđović, R., Stanković, S., Todorović, G.,& Nedić, N.. (2012). Disruption of attractant properties of potato foliage on Leptinotarsa decemlineata Say by the use of Salvia officinalis L. essential oil. in Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries
Association for Medicinal and Aromatic Plants of Southeast European Countries (AMAPSEEC) & Institute for Medicinal Plant Research “Dr Josif Pančić”, Belgrade, Srbija., 7.
https://hdl.handle.net/21.15107/rcub_rimsi_2803

Kostić M, Kostić I, Marković T, Jevđović R, Stanković S, Todorović G, Nedić N. Disruption of attractant properties of potato foliage on Leptinotarsa decemlineata Say by the use of Salvia officinalis L. essential oil. in Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries. 2012;7.
https://hdl.handle.net/21.15107/rcub_rimsi_2803 .

Kostić, Miroslav, Kostić, Igor, Marković, Tatjana, Jevđović, Radosav, Stanković, Slađan, Todorović, Goran, Nedić, Nebojša, "Disruption of attractant properties of potato foliage on Leptinotarsa decemlineata Say by the use of Salvia officinalis L. essential oil" in Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries, 7 (2012),
https://hdl.handle.net/21.15107/rcub_rimsi_2803 .