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Ministry of Education and Science of Republic of SerbiaMinistry of Education, Science & Technological Development, Serbia

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Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates

Zabel, Anton; Stanković, Slađan; Kostic, Miroslav; Rahović, Dragan; Tomic, Vedran; Kostić, Igor; Alkhammas, Iman Omar

(Ars Docendi, Bucharest, 2017)

TY  - JOUR
AU  - Zabel, Anton
AU  - Stanković, Slađan
AU  - Kostic, Miroslav
AU  - Rahović, Dragan
AU  - Tomic, Vedran
AU  - Kostić, Igor
AU  - Alkhammas, Iman Omar
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1025
AB  - The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.
PB  - Ars Docendi, Bucharest
T2  - Romanian Biotechnological Letters
T1  - Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates
EP  - 12596
IS  - 3
SP  - 12584
VL  - 22
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1025
ER  - 
@article{
author = "Zabel, Anton and Stanković, Slađan and Kostic, Miroslav and Rahović, Dragan and Tomic, Vedran and Kostić, Igor and Alkhammas, Iman Omar",
year = "2017",
abstract = "The resistance levels of the Colorado potato beetle (CPB) in Serbia to organophosphorus (OP) and carbamate (C) insecticides varied by population. The resistance level of populations to OPs indescending order was: Futog, Ratari, Dobanovci and Pester. Most resistant to carbarylwere populations Dobanovci and Ratari, followed by Futog, Kaona, and Pester, respectively. The order of resistance levels for OPs and C was completely opposite. Experiments showed that acetylcholinesterase (AChE) activity of CPB was verypronounced and easily measured. At a constant AChE concentration, increasing thesubstrate concentration will cause a positive, linear and dependent increase in thereaction. The same applies in the reaction with constant substrate concentration andincreased enzyme concentrations. AChE activity is significantly affected not only bylocation but also by substrate concentration (acetylthiocholine iodide ATChI). At low substrate concentrations, the highest AChE activity was determined in population Dobanovci, followed by Kaona, Ratari and Futog, respectively. At high substrate concentrations, however, the highest AChE activity was determined in population Ratari and then in Dobanovci and Futog. The lowest rate was in population Kaona. Considering that ATChI (substrate) in increased concentrations inhibits normal AChE activity, it can be concluded that altered AChE affected the change in the populationorder. The testing of AChE in the presence of high concentrations of substrate showsthe portion of altered AChE is largest in population Ratari, and yet considerably smallerin Dobanovci, Futog and Kaona. The total AChE activity is in correlation with thedetermined resistance to carbamates.",
publisher = "Ars Docendi, Bucharest",
journal = "Romanian Biotechnological Letters",
title = "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates",
pages = "12596-12584",
number = "3",
volume = "22",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1025"
}
Zabel, A., Stanković, S., Kostic, M., Rahović, D., Tomic, V., Kostić, I.,& Alkhammas, I. O.. (2017). Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters
Ars Docendi, Bucharest., 22(3), 12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025
Zabel A, Stanković S, Kostic M, Rahović D, Tomic V, Kostić I, Alkhammas IO. Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates. in Romanian Biotechnological Letters. 2017;22(3):12584-12596.
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .
Zabel, Anton, Stanković, Slađan, Kostic, Miroslav, Rahović, Dragan, Tomic, Vedran, Kostić, Igor, Alkhammas, Iman Omar, "Acetylcholinesterase [AChE] Activity of Colorado Potato Beetle Populations in Serbia Resistant to Carbamates and Organophosphates" in Romanian Biotechnological Letters, 22, no. 3 (2017):12584-12596,
https://hdl.handle.net/21.15107/rcub_rimsi_1025 .