TY  - CONF
AU  - Popović, Tatjana
AU  - Kostić, Igor
AU  - Milićević, Zoran
AU  - Gasic, Katarina
AU  - Kostić, Miroslav
AU  - Dervisevic, Marina
AU  - Krnjajić, Slobodan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2747
AB  - Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.
PB  - East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska
C3  - Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
T1  - Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum
EP  - 1383
SP  - 1377
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2747
ER  - 

@conference{
author = "Popović, Tatjana and Kostić, Igor and Milićević, Zoran and Gasic, Katarina and Kostić, Miroslav and Dervisevic, Marina and Krnjajić, Slobodan",
year = "2017",
abstract = "Bacterial soft-rot disease caused by Pectobacterium carotovorum subsp. carotovorum is a
very destructive disease with a diverse host range in agricultural crops, during the plant
growth in the field and in storage. Control, usually based on the use of chemical products, is
not satisfactory because of their harmful effect on human health and environment, as well as
the possibility for pathogen resistance development. Therefore, developing of new natural
products (such as essential oils-EOs, plant extracts etc.) with a sufficient efficacy in control of
the disease was imposed. The current study included in vitro testing of bactericidal activity of
51 different oils against P. c. subsp. carotovorum, using an agar-diffusion assay. Bacterial
suspension was mixed in Nutrient Agar to final concentration of 107
-108
cells/mL and poured
in sterilized Petri plates (ø 90 mm). After media solidified, sterile filter paper discs (ø 5 mm)
were placed on the surface of the Petri plates and supplemented with 20 µL of each tested
oils. The experiment was performed in a completely randomized design. The results were
expressed as a width of inhibition zone (mm) and analyzed by one-factor analysis of variance
by using the software package Statistica 7.0 (StatSoft, Inc.). Results showed that the 7 most
efficient EOs against P. c. subsp. carotovorum (Thymus vulgaris, Cinnamomum cassia,
Cassia angustifolia, Origanum vulgare, Boswellia serrata, Eucalyptus globulus, Satureja
montana, respectively) achieved inhibition zone greater than 20 mm. Inhibition zones in the
range of 15-20 mm were achieved by 8, 10-15 mm by 9, and less than 10 mm by 6 EOs. The
rest of 21 Eos did not show any bactericidal effect.",
publisher = "East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska",
journal = "Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina",
title = "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum",
pages = "1383-1377",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2747"
}

Popović, T., Kostić, I., Milićević, Z., Gasic, K., Kostić, M., Dervisevic, M.,& Krnjajić, S.. (2017). Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina
East Sarajevo: University of East Sarajevo, Faculty of Agriculture, Republic of Srpska., 8, 1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747

Popović T, Kostić I, Milićević Z, Gasic K, Kostić M, Dervisevic M, Krnjajić S. Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum. in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina. 2017;8:1377-1383.
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .

Popović, Tatjana, Kostić, Igor, Milićević, Zoran, Gasic, Katarina, Kostić, Miroslav, Dervisevic, Marina, Krnjajić, Slobodan, "Essential oils as an alternative bactericides against soft-rot bacteria, Pectobacterium carotovorum subsp. carotovorum" in Book of proceedings: VIII International Scientific Agriculture Symposium“AGROSYM 2017”, Jahorina, 8 (2017):1377-1383,
https://hdl.handle.net/21.15107/rcub_rimsi_2747 .