TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2707
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.
PB  - MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - https://doi.org/10.3390/plants11243587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "https://doi.org/10.3390/plants11243587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
MDPI., 11(24).
https://doi.org/https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24).
doi:https://doi.org/10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022),
https://doi.org/https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Kostic, Miroslav
AU  - Seslija-Jovanović, Darka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1533
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitasević-Jovanović, Sanja and Kostic, Miroslav and Seslija-Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitasević-Jovanović, S., Kostic, M.,& Seslija-Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Frontiers Media Sa, Lausanne., 13.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitasević-Jovanović S, Kostic M, Seslija-Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitasević-Jovanović, Sanja, Kostic, Miroslav, Seslija-Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022),
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Seslija-Jovanović, Darka
AU  - Kostic, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1404
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Seslija-Jovanović, Darka and Kostic, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood forests. In the search for an environmentally safe means of its control, we assessed the impact of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality, molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with commercial insecticide NeemAzal(R)-T/S (neem). The main compounds in the Eos were trans-anethole in anise; carvone, limonene, and alpha-phellandrene in dill; and trans-anethole and fenchone in fennel seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%, anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs were more effective in reducing growth rate, approximate digestibility and efficiency of conversion of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used for gypsy moth control.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194"
}

Kostić, I., Lazarević, J., Seslija-Jovanović, D., Kostic, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel
MDPI, Basel., 10(10).
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Seslija-Jovanović D, Kostic M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants-Basel. 2021;10(10).
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Seslija-Jovanović, Darka, Kostic, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants-Basel, 10, no. 10 (2021),
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Seslija-Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Calic, Dusica D
AU  - Stanković, Sladan
AU  - Kostic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1506
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
EP  - 199
IS  - 2
SP  - 190
VL  - 111
DO  - 10.1017/S0007485320000504
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Seslija-Jovanović, Darka and Krnjajić, Slobodan and Calic, Dusica D and Stanković, Sladan and Kostic, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
pages = "199-190",
number = "2",
volume = "111",
doi = "10.1017/S0007485320000504"
}

Lazarević, J., Kostić, I., Milanović, S., Seslija-Jovanović, D., Krnjajić, S., Calic, D. D., Stanković, S.,& Kostic, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 111(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Seslija-Jovanović D, Krnjajić S, Calic DD, Stanković S, Kostic M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;111(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Seslija-Jovanović, Darka, Krnjajić, Slobodan, Calic, Dusica D, Stanković, Sladan, Kostic, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 111, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Seslija-Jovanović, Darka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1320
AB  - Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - MDPI, Basel
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostic, Miroslav and Vuleta, Ana and Manitasević-Jovanović, Sanja and Seslija-Jovanović, Darka",
year = "2020",
abstract = "Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "MDPI, Basel",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostic, M., Vuleta, A., Manitasević-Jovanović, S.,& Seslija-Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
MDPI, Basel., 11(9).
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostic M, Vuleta A, Manitasević-Jovanović S, Seslija-Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9).
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostic, Miroslav, Vuleta, Ana, Manitasević-Jovanović, Sanja, Seslija-Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020),
https://doi.org/10.3390/insects11090563 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Popović, Marija M.
AU  - Dobrosavljević, Jovan N.
AU  - Kostić, Igor
AU  - Lazarević, Jelica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1379
AB  - Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress
EP  - 69
IS  - 1
SP  - 63
VL  - 72
DO  - 10.2298/ABS191106067M
ER  - 

@article{
author = "Milanović, Slobodan and Popović, Marija M. and Dobrosavljević, Jovan N. and Kostić, Igor and Lazarević, Jelica",
year = "2020",
abstract = "Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash ( CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress",
pages = "69-63",
number = "1",
volume = "72",
doi = "10.2298/ABS191106067M"
}

Milanović, S., Popović, M. M., Dobrosavljević, J. N., Kostić, I.,& Lazarević, J.. (2020). Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(1), 63-69.
https://doi.org/10.2298/ABS191106067M

Milanović S, Popović MM, Dobrosavljević JN, Kostić I, Lazarević J. Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences. 2020;72(1):63-69.
doi:10.2298/ABS191106067M .

Milanović, Slobodan, Popović, Marija M., Dobrosavljević, Jovan N., Kostić, Igor, Lazarević, Jelica, "Desperate times call for desperate measures: short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress" in Archives of Biological Sciences, 72, no. 1 (2020):63-69,
https://doi.org/10.2298/ABS191106067M . .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Calic, Dusica D
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1343
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control
EP  - 11967
IS  - 11
SP  - 11958
VL  - 27
DO  - 10.1007/s11356-020-07825-1
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Calic, Dusica D",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality ( lt  10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control",
pages = "11967-11958",
number = "11",
volume = "27",
doi = "10.1007/s11356-020-07825-1"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Calic, D. D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(11), 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Calic DD. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control. in Environmental Science and Pollution Research. 2020;27(11):11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Calic, Dusica D, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control" in Environmental Science and Pollution Research, 27, no. 11 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostic, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjic, Anda
AU  - Kostić, Igor
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1252
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans
EP  - 676
IS  - 4
SP  - 665
VL  - 71
DO  - 10.2298/ABS190617049J
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostic, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjic, Anda and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, alpha-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and a-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans",
pages = "676-665",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J"
}

Jevremović, S., Lazarević, J., Kostic, M., Krnjajić, S., Ugrenović, V., Radonjic, A.,& Kostić, I.. (2019). Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostic M, Krnjajić S, Ugrenović V, Radonjic A, Kostić I. Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostic, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjic, Anda, Kostić, Igor, "Contact application of Lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Radojković, Aleksandar
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
AU  - Mitrović, Jelena
AU  - Kostic, Miroslav
AU  - Novaković, Tatjana
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1170
AB  - For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (alpha-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8  lt  F/O-0.5  lt  F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Stored Products Research
T1  - Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)
EP  - 54
SP  - 45
VL  - 77
DO  - 10.1016/j.jspr.2018.02.006
ER  - 

@article{
author = "Lazarević, Jelica and Radojković, Aleksandar and Kostić, Igor and Krnjajić, Slobodan and Mitrović, Jelena and Kostic, Miroslav and Novaković, Tatjana and Branković, Zorica and Branković, Goran",
year = "2018",
abstract = "For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (alpha-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8  lt  F/O-0.5  lt  F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Stored Products Research",
title = "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)",
pages = "54-45",
volume = "77",
doi = "10.1016/j.jspr.2018.02.006"
}

Lazarević, J., Radojković, A., Kostić, I., Krnjajić, S., Mitrović, J., Kostic, M., Novaković, T., Branković, Z.,& Branković, G.. (2018). Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research
Pergamon-Elsevier Science Ltd, Oxford., 77, 45-54.
https://doi.org/10.1016/j.jspr.2018.02.006

Lazarević J, Radojković A, Kostić I, Krnjajić S, Mitrović J, Kostic M, Novaković T, Branković Z, Branković G. Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2018;77:45-54.
doi:10.1016/j.jspr.2018.02.006 .

Lazarević, Jelica, Radojković, Aleksandar, Kostić, Igor, Krnjajić, Slobodan, Mitrović, Jelena, Kostic, Miroslav, Novaković, Tatjana, Branković, Zorica, Branković, Goran, "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 77 (2018):45-54,
https://doi.org/10.1016/j.jspr.2018.02.006 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Jankovic-Tomanic, Milena
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/990
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.
PB  - Wiley, Hoboken
T2  - Entomologia Experimentalis Et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
EP  - 162
IS  - 2
SP  - 152
VL  - 158
DO  - 10.1111/eea.12388
ER  - 

@article{
author = "Milanović, Slobodan and Jankovic-Tomanic, Milena and Kostić, Igor and Kostic, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, frequently encounter spatial and temporal variations in diet quality. Such variation favoured the evolution of high behavioural and physiological plasticity which, depending on forest stand composition, enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable habitat, interspecific and intraspecific host quality variation may provoke significant variation in gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.) (TH and HT groups), under controlled laboratory conditions, and compared larval performance between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hungarian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT larvae. It appeared that the lower growth in the TH group than in the TT group was caused by both behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet experience influenced the sensitivity of themost examined traits to less suitableHungarian oaks, suggesting the development of behavioural and physiological adjustments. Our results indicate that lower risks of defoliation by gypsy mothmight be expected inmixed stands with a higher proportion of Hungarian oak.",
publisher = "Wiley, Hoboken",
journal = "Entomologia Experimentalis Et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
pages = "162-152",
number = "2",
volume = "158",
doi = "10.1111/eea.12388"
}

Milanović, S., Jankovic-Tomanic, M., Kostić, I., Kostic, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata
Wiley, Hoboken., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Jankovic-Tomanic M, Kostić I, Kostic M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis Et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Jankovic-Tomanic, Milena, Kostić, Igor, Kostic, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis Et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .