TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1454
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
EP  - 3245
IS  - 7
SP  - 3233
VL  - 12
DO  - 10.1039/d1fo00447f
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpic-Aradski, Ana and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
pages = "3245-3233",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f"
}

Oalde, M., Kolarević, S., Živković, J., Alimpic-Aradski, A., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Marin, P. D., Savikin, K., Vukovic-Gacic, B.,& Duletic-Lausević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Soc Chemistry, Cambridge., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalde M, Kolarević S, Živković J, Alimpic-Aradski A, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Marin PD, Savikin K, Vukovic-Gacic B, Duletic-Lausević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpic-Aradski, Ana, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Duletic-Lausević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Pavlović, Mariana Oalde
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović-Marić, Jovana
AU  - Lunic, Tanja
AU  - Mandic, Marija
AU  - Kracun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Bozić-Nedeljković, Biljana
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1502
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
ER  - 

@article{
author = "Pavlović, Mariana Oalde and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Jovanović-Marić, Jovana and Lunic, Tanja and Mandic, Marija and Kracun-Kolarević, Margareta and Živković, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Bozić-Nedeljković, Biljana and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306"
}

Pavlović, M. O., Kolarević, S., Đorđević Aleksić, J., Jovanović-Marić, J., Lunic, T., Mandic, M., Kracun-Kolarević, M., Živković, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K., Vukovic-Gacic, B., Bozić-Nedeljković, B.,& Duletic-Lausević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112306

Pavlović MO, Kolarević S, Đorđević Aleksić J, Jovanović-Marić J, Lunic T, Mandic M, Kracun-Kolarević M, Živković J, Alimpic-Aradski A, Marin PD, Savikin K, Vukovic-Gacic B, Bozić-Nedeljković B, Duletic-Lausević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112306 .

Pavlović, Mariana Oalde, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Jovanović-Marić, Jovana, Lunic, Tanja, Mandic, Marija, Kracun-Kolarević, Margareta, Živković, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Bozić-Nedeljković, Biljana, Duletic-Lausević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112306 . .

TY  - CHAP
AU  - Stankovic, Sladjan
AU  - Kostić, MIroslav
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2425
AB  - Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.
PB  - IntechOpen
T2  - In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
T1  - Practical Approaches to Pest Control: The Use of Natural Compounds
EP  - 18
SP  - 1
DO  - 10.5772/intechopen.91792
ER  - 

@inbook{
author = "Stankovic, Sladjan and Kostić, MIroslav and Kostić, Igor and Krnjajić, Slobodan",
year = "2020",
abstract = "Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.",
publisher = "IntechOpen",
journal = "In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen",
booktitle = "Practical Approaches to Pest Control: The Use of Natural Compounds",
pages = "18-1",
doi = "10.5772/intechopen.91792"
}

Stankovic, S., Kostić, M., Kostić, I.,& Krnjajić, S.. (2020). Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
IntechOpen., 1-18.
https://doi.org/10.5772/intechopen.91792

Stankovic S, Kostić M, Kostić I, Krnjajić S. Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen. 2020;:1-18.
doi:10.5772/intechopen.91792 .

Stankovic, Sladjan, Kostić, MIroslav, Kostić, Igor, Krnjajić, Slobodan, "Practical Approaches to Pest Control: The Use of Natural Compounds" in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen (2020):1-18,
https://doi.org/10.5772/intechopen.91792 . .

TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena C.
AU  - Vukovic-Gacic, Branka
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Duletic-Lausević, Sonja N.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1326
AB  - The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.
PB  - Elsevier, Amsterdam
T2  - Saudi Pharmaceutical Journal
T1  - The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro
EP  - 1604
IS  - 12
SP  - 1592
VL  - 28
DO  - 10.1016/j.jsps.2020.10.006
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena C. and Vukovic-Gacic, Branka and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Duletic-Lausević, Sonja N.",
year = "2020",
abstract = "The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Pharmaceutical Journal",
title = "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro",
pages = "1604-1592",
number = "12",
volume = "28",
doi = "10.1016/j.jsps.2020.10.006"
}

Oalde, M., Kolarević, S., Živković, J. C., Vukovic-Gacic, B., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K.,& Duletic-Lausević, S. N.. (2020). The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal
Elsevier, Amsterdam., 28(12), 1592-1604.
https://doi.org/10.1016/j.jsps.2020.10.006

Oalde M, Kolarević S, Živković JC, Vukovic-Gacic B, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Alimpic-Aradski A, Marin PD, Savikin K, Duletic-Lausević SN. The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal. 2020;28(12):1592-1604.
doi:10.1016/j.jsps.2020.10.006 .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena C., Vukovic-Gacic, Branka, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Duletic-Lausević, Sonja N., "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro" in Saudi Pharmaceutical Journal, 28, no. 12 (2020):1592-1604,
https://doi.org/10.1016/j.jsps.2020.10.006 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostic, Miroslav
AU  - Vuleta, Ana
AU  - Manitasević-Jovanović, Sanja
AU  - Seslija-Jovanović, Darka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1320
AB  - Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - MDPI, Basel
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostic, Miroslav and Vuleta, Ana and Manitasević-Jovanović, Sanja and Seslija-Jovanović, Darka",
year = "2020",
abstract = "Simple Summary Compounds of botanical origin, including essential oils (EOs), which coevolved as plant defense mechanism against herbivores and pathogens have been proposed as a promising strategy for post-harvest control of stored product insects. Despite several drawbacks, such as low stability, phytotoxicity and food odor changes at high concentrations, EOs are believed to be safe for human and environmental health and thus have an advantage comparing to conventional insecticides. The present study was aimed to examine acute toxicity and effects of thyme (Thymus vulgaris) EO on longevity, behavior and physiology of the bean weevil (Acanthoscelides obtectus), a major pest of stored bean seeds. We found that exposure to thyme oil reduced adult survival and longevity and induced damages to lipids and proteins in a concentration-dependent manner. Sublethal EO concentrations also deterred female egg laying and strongly inhibited adult emergence. Efficacy of such low EO concentrations in suppression of adult emergence implies that thyme EO might be cost-effective and environmentally low risk botanical insecticide for bean seed protection against A. obtectus. The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "MDPI, Basel",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostic, M., Vuleta, A., Manitasević-Jovanović, S.,& Seslija-Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
MDPI, Basel., 11(9).
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostic M, Vuleta A, Manitasević-Jovanović S, Seslija-Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9).
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostic, Miroslav, Vuleta, Ana, Manitasević-Jovanović, Sanja, Seslija-Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020),
https://doi.org/10.3390/insects11090563 . .