TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3188
AB  - Гастроинтестиналне нематоде представљају веома значајне паразите домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анорексије, анемије, јаке дијареје и губитка протеина што све заједно може довести и до угинућа. Комерцијални лекови попут бензимидазола и макроцикличних лактона су се деценијама са успехом користили за контролу ових паразита. Међутим, нерационална примена ових лекова је довела до развоја антихелминтичке резистенције, опадања њихове ефикасности и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, а присутан је и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а у циљу успостављања интегрисаног приступа који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани, циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно отпорних на нематоде, рационалног управљања пашњацима, избалансиране исхране уз допунска хранива, биолошке контроле (употреба гљива, бактерија и слично), развоја вакцина као и употребе ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља). Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће успорити развој и ширење антихелминтичке резистенције, као и постићи одрживу контрола ових паразита и смањење економских губитака.
AB  - Gastrointestinal nematodes are very important parasites of domestic animals, especially in small ruminants. In sheep, diseases usually have a subclinical form with a decrease in body weight and production, and sometimes there can be visible clinical symptoms such as anorexia, anemia, severe diarrhea and protein loss, all of which together can lead to death. Commercial drugs such as benzimidazole and macrocyclic lactones have been successfully used for decades to control these parasites. However, the irrational application of these drugs has led to the development of anthelmintic resistance, a decrease in their effectiveness and consequent economic losses that threaten the sustainability of sheep breeding. In addition, the use of commercial preparations is also associated with the residues in animal products and the environment, and there is also an increase in the price of these preparations. For this reason, novel strategies for controlling of these parasites are actively seeking, with the goal of establishing an integrated approach that involves the application of several different strategies. On the one hand, these include strategies for the rational application of commercial preparations based on refugia (targeted treatments, targeted selective treatments), as well as the combination and rotation of anthelmintics from different chemical groups. On the other hand, an integrated approach implies the use of different alternative strategies such as genetic selection of animals naturally resistant to nematodes, rational management of pastures, balanced nutrition with supplementary nutrients, biological control (use of fungi, bacteria, etc.), development of vaccines as well as the use of botanical preparations (plants and their products such as extracts and essential oils). Various studies have proven the effectiveness of the mentioned strategies in the control of gastrointestinal nematodes of sheep, whereby the best results are achieved by their combined application. Thus, it is possible to slow down the development and spread of anthelmintic resistance, as well as achieve sustainable control of these parasites and reduce economic losses.
PB  - ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука
T2  - Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska
T1  - Савремене стратегије контроле гастроинтестиналних нематода код оваца
T1  - Novel Strategies for the Control of Gastrointestinal Nematodes in Sheep
EP  - 245
IS  - 1-2
SP  - 209
VL  - XXIII
DO  - 10.7251/VETJSR2301209S
DO  - 10.7251/VETJEN2301227S
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir",
year = "2023",
abstract = "Гастроинтестиналне нематоде представљају веома значајне паразите домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анорексије, анемије, јаке дијареје и губитка протеина што све заједно може довести и до угинућа. Комерцијални лекови попут бензимидазола и макроцикличних лактона су се деценијама са успехом користили за контролу ових паразита. Међутим, нерационална примена ових лекова је довела до развоја антихелминтичке резистенције, опадања њихове ефикасности и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, а присутан је и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а у циљу успостављања интегрисаног приступа који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани, циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно отпорних на нематоде, рационалног управљања пашњацима, избалансиране исхране уз допунска хранива, биолошке контроле (употреба гљива, бактерија и слично), развоја вакцина као и употребе ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља). Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће успорити развој и ширење антихелминтичке резистенције, као и постићи одрживу контрола ових паразита и смањење економских губитака., Gastrointestinal nematodes are very important parasites of domestic animals, especially in small ruminants. In sheep, diseases usually have a subclinical form with a decrease in body weight and production, and sometimes there can be visible clinical symptoms such as anorexia, anemia, severe diarrhea and protein loss, all of which together can lead to death. Commercial drugs such as benzimidazole and macrocyclic lactones have been successfully used for decades to control these parasites. However, the irrational application of these drugs has led to the development of anthelmintic resistance, a decrease in their effectiveness and consequent economic losses that threaten the sustainability of sheep breeding. In addition, the use of commercial preparations is also associated with the residues in animal products and the environment, and there is also an increase in the price of these preparations. For this reason, novel strategies for controlling of these parasites are actively seeking, with the goal of establishing an integrated approach that involves the application of several different strategies. On the one hand, these include strategies for the rational application of commercial preparations based on refugia (targeted treatments, targeted selective treatments), as well as the combination and rotation of anthelmintics from different chemical groups. On the other hand, an integrated approach implies the use of different alternative strategies such as genetic selection of animals naturally resistant to nematodes, rational management of pastures, balanced nutrition with supplementary nutrients, biological control (use of fungi, bacteria, etc.), development of vaccines as well as the use of botanical preparations (plants and their products such as extracts and essential oils). Various studies have proven the effectiveness of the mentioned strategies in the control of gastrointestinal nematodes of sheep, whereby the best results are achieved by their combined application. Thus, it is possible to slow down the development and spread of anthelmintic resistance, as well as achieve sustainable control of these parasites and reduce economic losses.",
publisher = "ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука",
journal = "Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska",
title = "Савремене стратегије контроле гастроинтестиналних нематода код оваца, Novel Strategies for the Control of Gastrointestinal Nematodes in Sheep",
pages = "245-209",
number = "1-2",
volume = "XXIII",
doi = "10.7251/VETJSR2301209S, 10.7251/VETJEN2301227S"
}

Štrbac, F., Krnjajić, S., Stojanović, D.,& Ratajac, R.. (2023). Савремене стратегије контроле гастроинтестиналних нематода код оваца. in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska
ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука., XXIII(1-2), 209-245.
https://doi.org/10.7251/VETJSR2301209S

Štrbac F, Krnjajić S, Stojanović D, Ratajac R. Савремене стратегије контроле гастроинтестиналних нематода код оваца. in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska. 2023;XXIII(1-2):209-245.
doi:10.7251/VETJSR2301209S .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, "Савремене стратегије контроле гастроинтестиналних нематода код оваца" in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska, XXIII, no. 1-2 (2023):209-245,
https://doi.org/10.7251/VETJSR2301209S . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2002
AB  - Гастроинтестиналне нематоде представљају веома важне паразите код домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анемије, јаке дијареје, анорексије и губитака протеина што све заједно може довести и до угинућа. Комерцијални препарати попут бензимидазола и макроцикличних
лактона су се деценијама са успехом користили у контроли ових паразита. Међутим, њихова нерационална примена је довела до развоја антихелминтичке резистенције, опадања ефикасности лекова и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, при чему је присутан и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а које се ослањају на интегрисан приступ који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани и циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са
друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно резистентних на нематоде, рационално управљање пашњацима, избалансирану исхрану уз допунска хранива, биолошку контролу (употребу гљива, бактерија и слично), развој вакцина као и употребу ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља).
Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће постићи успоравање развоја и ширења антихелминтичке резистенције, као и одрживу контролу ових паразита и смањење економских губитака.
AB  - Gastrointestinal nematodes represent very important parasites in domestic
animals, especially in small ruminants. In sheep, diseases usually have a
subclinical course with a decrease in body weight and production, whereby
sometimes visible clinical symptoms such as anaemia, severe diarrhea, anorexia
and protein loss with a possible fatal outcome may occur. Commercial drugs such
as benzimidasoles and macrocyclic lactones have been used successfully for
decades in the control of these parasites. However, their inappropriate use has led
to the development of anthelmintic resistance, decreasing efficacy of drugs and
consequent economic losses that endanger the sustainability of sheep farming. In
addition, application of commercial preparations is also associated with the
residues in animal products and the environment, while the increase in their price
is also present. Therefore, novel strategies for the control of these parasites are
actively sought, which rely on an integrated approach that involves the application
of several different strategies. On the one hand, these include strategies for the
rational application of commercial drugs based on refugia (target treatments and
target selective treatments), as well as the combination and rotation of
anthelmintics from different chemical groups. On the other hand, an integrated
approach implies the use of various alternative strategies, such as genetic
selection of animals naturally resistant to nematodes, rational management of
pastures, balanced nutrition with supplementary nutrients, biological control (use
of fungi, bacteria, etc.), development of vaccines, as well as the use of botanical
preparations (plants and their products such as extracts and essential oils).
Various studies have proven the effectiveness of the mentioned strategies in the
control of gastrointestinal nematodes of sheep, whereby the best results are
achieved by their combined application. In this way, it is possible to slow down
the development and spread of anthelmintic resistance, and to achieve sustainable
control of these parasites and reduction of economic losses.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле
T1  - GASTROINTESTINAL NEMATODES IN SHEEP - CURRENT SITUATION AND MODERN CONTROL STRATEGIES
EP  - 40
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2002
ER  - 

@conference{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir",
year = "2023",
abstract = "Гастроинтестиналне нематоде представљају веома важне паразите код домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анемије, јаке дијареје, анорексије и губитака протеина што све заједно може довести и до угинућа. Комерцијални препарати попут бензимидазола и макроцикличних
лактона су се деценијама са успехом користили у контроли ових паразита. Међутим, њихова нерационална примена је довела до развоја антихелминтичке резистенције, опадања ефикасности лекова и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, при чему је присутан и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а које се ослањају на интегрисан приступ који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани и циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са
друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно резистентних на нематоде, рационално управљање пашњацима, избалансирану исхрану уз допунска хранива, биолошку контролу (употребу гљива, бактерија и слично), развој вакцина као и употребу ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља).
Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће постићи успоравање развоја и ширења антихелминтичке резистенције, као и одрживу контролу ових паразита и смањење економских губитака., Gastrointestinal nematodes represent very important parasites in domestic
animals, especially in small ruminants. In sheep, diseases usually have a
subclinical course with a decrease in body weight and production, whereby
sometimes visible clinical symptoms such as anaemia, severe diarrhea, anorexia
and protein loss with a possible fatal outcome may occur. Commercial drugs such
as benzimidasoles and macrocyclic lactones have been used successfully for
decades in the control of these parasites. However, their inappropriate use has led
to the development of anthelmintic resistance, decreasing efficacy of drugs and
consequent economic losses that endanger the sustainability of sheep farming. In
addition, application of commercial preparations is also associated with the
residues in animal products and the environment, while the increase in their price
is also present. Therefore, novel strategies for the control of these parasites are
actively sought, which rely on an integrated approach that involves the application
of several different strategies. On the one hand, these include strategies for the
rational application of commercial drugs based on refugia (target treatments and
target selective treatments), as well as the combination and rotation of
anthelmintics from different chemical groups. On the other hand, an integrated
approach implies the use of various alternative strategies, such as genetic
selection of animals naturally resistant to nematodes, rational management of
pastures, balanced nutrition with supplementary nutrients, biological control (use
of fungi, bacteria, etc.), development of vaccines, as well as the use of botanical
preparations (plants and their products such as extracts and essential oils).
Various studies have proven the effectiveness of the mentioned strategies in the
control of gastrointestinal nematodes of sheep, whereby the best results are
achieved by their combined application. In this way, it is possible to slow down
the development and spread of anthelmintic resistance, and to achieve sustainable
control of these parasites and reduction of economic losses.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле, GASTROINTESTINAL NEMATODES IN SHEEP - CURRENT SITUATION AND MODERN CONTROL STRATEGIES",
pages = "40-39",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2002"
}

Štrbac, F., Krnjajić, S., Stojanović, D.,& Ratajac, R.. (2023). Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле. in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 39-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2002

Štrbac F, Krnjajić S, Stojanović D, Ratajac R. Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле. in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2023;:39-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2002 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, "Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле" in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2023):39-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2002 .

TY  - CONF
AU  - Grujić, Tara
AU  - Saljnikov, Elmira
AU  - Jovković, Marina
AU  - Belanovic Simic, Snezana
AU  - Krnjajić, Slobodan
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2731
AB  - Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. One of the first investigations of plastic and microplastic (MP) in the soil on the territory  of Serbia is currently underway and is being carried out within the project "Evaluation of the microplastic in the Soils of Serbia – EMIPLAST – SoS" funded by the Science Fund of the Republic of Serbia. The aim of the research is to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. The examination of the impact of MP on the soil is being carried out through a comparative analysis of samples from localities that are and are not exposed to MP pollution. Sampling was done in three seasonal repetitions in the 2022 and will be done in the same way in 2023 in all selected plots. Microbial respiration is measured from all samples using the alkaline trap method as an indicator of microbial activity. The parameters related to the soil structure are not variable in such a short time frame, which is why they were determined at the beginning and will be determined at the end of the experimental period: mechanical composition, volumetric mass, specific mass, porosity, aggregate stability and organic matter content. Soil parameters that may affect aggregate stability such as pH, electrical conductivity and total carbon content, as well as soil nutritional status (N, P2O5, K2O, Cu, Zn, Mn and CaCO3) were determined.
These parameters are used to characterize the soil at the research sites. Preliminary results showed that some soil properties are significantly affected by the presence of plastic materials. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.
PB  - University of Belgrade, Faculty of Geography
PB  - University of Banja Luka, Faculty of Natural Sciences and Mathematics
PB  - Serbian Academy of Sciences and Arts, Geographical Institute “Jovan Cvijić”, Serbia
PB  - Faculty of Geography, Lomonosov Moscow State University
PB  - Russian Geographical Society
PB  - The World Association of Soil and Water Conservation (WASWAC) a
C3  - International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS
T1  - ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT
EP  - 39
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2731
ER  - 

@conference{
author = "Grujić, Tara and Saljnikov, Elmira and Jovković, Marina and Belanovic Simic, Snezana and Krnjajić, Slobodan and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. One of the first investigations of plastic and microplastic (MP) in the soil on the territory  of Serbia is currently underway and is being carried out within the project "Evaluation of the microplastic in the Soils of Serbia – EMIPLAST – SoS" funded by the Science Fund of the Republic of Serbia. The aim of the research is to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. The examination of the impact of MP on the soil is being carried out through a comparative analysis of samples from localities that are and are not exposed to MP pollution. Sampling was done in three seasonal repetitions in the 2022 and will be done in the same way in 2023 in all selected plots. Microbial respiration is measured from all samples using the alkaline trap method as an indicator of microbial activity. The parameters related to the soil structure are not variable in such a short time frame, which is why they were determined at the beginning and will be determined at the end of the experimental period: mechanical composition, volumetric mass, specific mass, porosity, aggregate stability and organic matter content. Soil parameters that may affect aggregate stability such as pH, electrical conductivity and total carbon content, as well as soil nutritional status (N, P2O5, K2O, Cu, Zn, Mn and CaCO3) were determined.
These parameters are used to characterize the soil at the research sites. Preliminary results showed that some soil properties are significantly affected by the presence of plastic materials. In order to establish the level of the negative impact of microplastics on soil properties and microbial activity in the longer term, the study is ongoing.",
publisher = "University of Belgrade, Faculty of Geography, University of Banja Luka, Faculty of Natural Sciences and Mathematics, Serbian Academy of Sciences and Arts, Geographical Institute “Jovan Cvijić”, Serbia, Faculty of Geography, Lomonosov Moscow State University, Russian Geographical Society, The World Association of Soil and Water Conservation (WASWAC) a",
journal = "International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS",
title = "ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2731"
}

Grujić, T., Saljnikov, E., Jovković, M., Belanovic Simic, S., Krnjajić, S.,& Marjanović, Ž.. (2023). ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT. in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS
University of Belgrade, Faculty of Geography., 39-39.
https://hdl.handle.net/21.15107/rcub_rimsi_2731

Grujić T, Saljnikov E, Jovković M, Belanovic Simic S, Krnjajić S, Marjanović Ž. ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT. in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS. 2023;:39-39.
https://hdl.handle.net/21.15107/rcub_rimsi_2731 .

Grujić, Tara, Saljnikov, Elmira, Jovković, Marina, Belanovic Simic, Snezana, Krnjajić, Slobodan, Marjanović, Žaklina, "ASSESSMENT OF PLASTIC POLLUTION OF THE SOIL ENVIRONMENT" in International conference on transboundary catchment erosion and pollution problems, BOOK OF ABSTRACTS (2023):39-39,
https://hdl.handle.net/21.15107/rcub_rimsi_2731 .

TY  - CONF
AU  - Krnjajić, Slobodan
AU  - Štrbac, Filip
AU  - Radović, Vesela
AU  - Stanković, Slađan
AU  - Tomić, Vedran
AU  - Stefanović, Slobodan
AU  - Knežević, Goran
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2435
AB  - Plastic has been recognized as one of the leading pollutants of all ecosystems. This question has been rarely raised in Serbia, although there is a significant problem with the disposal of communal and agricultural waste. Many wild landfills have been created in past decades in forests near the plasticulture practicing areas and rivers. Plastic materials are degraded by the influence of UV light and various environmental factors, creating smaller particles called microplastics, which are recognized as significantly dangerous to ecosystems. Alluvial planes have been the most endangered due to the horizontal and vertical fluvial activities of the nearby rivers. The influence of soil microplastics on insect communities has not been widely considered and that is why the project EMIPLAST-SoS has been created to investigate insect communities in polluted and unpolluted alluvial soils in Serbia. We compare them in forest and agricultural ecosystems on similar alluvial soils in three locations near three largest rivers in Serbia. Preliminary results from one-season sampling are considered. During the project the influence of the different seasons effects will be investigated. Additionally, we have investigated the rate of plastic decomposition by five insect species in lab conditions (Plodia interpunctella Hubner, Tenebrio molitor L., Zophobas morio Fabr., Achroia grisella Fabr. and Galleria mellonela L.). G. mellonela expressed the highest and the most diverse decomposition of different plastic sources like plastic bags, sponges and styrofoam. The analyses of the obtained plastic residues are still underway.
PB  - Hellenic Entomological Society
C3  - Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece
T1  - The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects
EP  - 422
SP  - 421
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2435
ER  - 

@conference{
author = "Krnjajić, Slobodan and Štrbac, Filip and Radović, Vesela and Stanković, Slađan and Tomić, Vedran and Stefanović, Slobodan and Knežević, Goran and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic has been recognized as one of the leading pollutants of all ecosystems. This question has been rarely raised in Serbia, although there is a significant problem with the disposal of communal and agricultural waste. Many wild landfills have been created in past decades in forests near the plasticulture practicing areas and rivers. Plastic materials are degraded by the influence of UV light and various environmental factors, creating smaller particles called microplastics, which are recognized as significantly dangerous to ecosystems. Alluvial planes have been the most endangered due to the horizontal and vertical fluvial activities of the nearby rivers. The influence of soil microplastics on insect communities has not been widely considered and that is why the project EMIPLAST-SoS has been created to investigate insect communities in polluted and unpolluted alluvial soils in Serbia. We compare them in forest and agricultural ecosystems on similar alluvial soils in three locations near three largest rivers in Serbia. Preliminary results from one-season sampling are considered. During the project the influence of the different seasons effects will be investigated. Additionally, we have investigated the rate of plastic decomposition by five insect species in lab conditions (Plodia interpunctella Hubner, Tenebrio molitor L., Zophobas morio Fabr., Achroia grisella Fabr. and Galleria mellonela L.). G. mellonela expressed the highest and the most diverse decomposition of different plastic sources like plastic bags, sponges and styrofoam. The analyses of the obtained plastic residues are still underway.",
publisher = "Hellenic Entomological Society",
journal = "Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece",
title = "The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects",
pages = "422-421",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2435"
}

Krnjajić, S., Štrbac, F., Radović, V., Stanković, S., Tomić, V., Stefanović, S., Knežević, G.,& Marjanović, Ž.. (2023). The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects. in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece
Hellenic Entomological Society., 421-422.
https://hdl.handle.net/21.15107/rcub_rimsi_2435

Krnjajić S, Štrbac F, Radović V, Stanković S, Tomić V, Stefanović S, Knežević G, Marjanović Ž. The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects. in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece. 2023;:421-422.
https://hdl.handle.net/21.15107/rcub_rimsi_2435 .

Krnjajić, Slobodan, Štrbac, Filip, Radović, Vesela, Stanković, Slađan, Tomić, Vedran, Stefanović, Slobodan, Knežević, Goran, Marjanović, Žaklina, "The Impact of Soil Microplastic on Insect Diversity and Plastic Decomposition by Insects" in Book of Abstracts of the XII European Congress Of Entomology, Crete, Greece (2023):421-422,
https://hdl.handle.net/21.15107/rcub_rimsi_2435 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stanković, Slađan
AU  - Tomić, Vedran
AU  - Ratajac, Radomir
AU  - Stojanović, Dragica
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2441
AB  - Gastrointestinal nematodes represent parasites of great importance in veterinary medicine, especially in grazing animals such as small ruminants. In most cases, these parasites cause subclinical diseases with losses in body weight, reduced feed intake and decreased production. However, high worm burdens may lead to severe clinical signs such as anorexia, anaemia, diarrhoea, protein loss, oedema, decreased immunity and fertility, which can lead to fatal outcomes. Therefore, gastrointestinal nematodes hinder sheep farming in different ways leading to high economic losses that are estimated at several hundred million euros in Europe, although it is difficult to quantify it precisely. An additional problem is the development of anthelmintic resistance in nematodes that occur as a result of irrational use of commercial drugs, whereby the main risk factors are overfrequent treatments, underdosing, the use of only one drug without combination or rotation etc. This leads to a decrease in their effectiveness, and consequently to even higher economic losses (an additional 35-40 million euros). Moreover, these costs will tend to increase in the future due to widespread of resistance and the occurrence of multiresistant strains. For these reasons, the focus of nowadays research is finding alternative solutions including genetic selection of naturally resistant animals, pasture management, dietary manipulation, biological control (use of nematophagous fungi, bacteria or earthworms and dung beetles) and the use of plant-based formulations such as extracts and essential oils. All of the above strategies have shown promise, although it appears that none of them could be used independently. Therefore, future strategies for the control of gastrointestinal nematodes should be based on an integrated approach. This implies the combination of mentioned alternatives with rational use of anthelmintics based on refugia strategies (target treatments and target selective treatments).
PB  - Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina
C3  - Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina
T1  - The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2441
ER  - 

@conference{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stanković, Slađan and Tomić, Vedran and Ratajac, Radomir and Stojanović, Dragica",
year = "2023",
abstract = "Gastrointestinal nematodes represent parasites of great importance in veterinary medicine, especially in grazing animals such as small ruminants. In most cases, these parasites cause subclinical diseases with losses in body weight, reduced feed intake and decreased production. However, high worm burdens may lead to severe clinical signs such as anorexia, anaemia, diarrhoea, protein loss, oedema, decreased immunity and fertility, which can lead to fatal outcomes. Therefore, gastrointestinal nematodes hinder sheep farming in different ways leading to high economic losses that are estimated at several hundred million euros in Europe, although it is difficult to quantify it precisely. An additional problem is the development of anthelmintic resistance in nematodes that occur as a result of irrational use of commercial drugs, whereby the main risk factors are overfrequent treatments, underdosing, the use of only one drug without combination or rotation etc. This leads to a decrease in their effectiveness, and consequently to even higher economic losses (an additional 35-40 million euros). Moreover, these costs will tend to increase in the future due to widespread of resistance and the occurrence of multiresistant strains. For these reasons, the focus of nowadays research is finding alternative solutions including genetic selection of naturally resistant animals, pasture management, dietary manipulation, biological control (use of nematophagous fungi, bacteria or earthworms and dung beetles) and the use of plant-based formulations such as extracts and essential oils. All of the above strategies have shown promise, although it appears that none of them could be used independently. Therefore, future strategies for the control of gastrointestinal nematodes should be based on an integrated approach. This implies the combination of mentioned alternatives with rational use of anthelmintics based on refugia strategies (target treatments and target selective treatments).",
publisher = "Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina",
journal = "Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina",
title = "The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2441"
}

Štrbac, F., Krnjajić, S., Stanković, S., Tomić, V., Ratajac, R.,& Stojanović, D.. (2023). The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies. in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina
Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..
https://hdl.handle.net/21.15107/rcub_rimsi_2441

Štrbac F, Krnjajić S, Stanković S, Tomić V, Ratajac R, Stojanović D. The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies. in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2441 .

Štrbac, Filip, Krnjajić, Slobodan, Stanković, Slađan, Tomić, Vedran, Ratajac, Radomir, Stojanović, Dragica, "The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies" in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2441 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Krnjajić, Slobodan
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2137
AB  - Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic
potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”
T1  - Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2137
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Krnjajić, Slobodan and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2023",
abstract = "Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic
potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”",
title = "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2137"
}

Štrbac, F., Simin, N., Orčić, D., Krnjajić, S., Bosco, A., Stojanović, D., Ratajac, R., Cringoli, G.,& Rinaldi, L.. (2023). Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 113-113.
https://hdl.handle.net/21.15107/rcub_rimsi_2137

Štrbac F, Simin N, Orčić D, Krnjajić S, Bosco A, Stojanović D, Ratajac R, Cringoli G, Rinaldi L. Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_rimsi_2137 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Krnjajić, Slobodan, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Cringoli, Giuseppe, Rinaldi, Laura, "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties" in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology” (2023):113-113,
https://hdl.handle.net/21.15107/rcub_rimsi_2137 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Rinaldi, Laura
AU  - Ciccone, Elena
AU  - Maurelli, Maria Paola
AU  - Cringoli, Giuseppe
AU  - Bosco, Antonio
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2105
AB  - Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.
PB  - Frontiers
T2  - Frontiers in Veterinary Science
T1  - In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1232570
VL  - 10
DO  - 10.3389/fvets.2023.1232570
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Rinaldi, Laura and Ciccone, Elena and Maurelli, Maria Paola and Cringoli, Giuseppe and Bosco, Antonio",
year = "2023",
abstract = "Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.",
publisher = "Frontiers",
journal = "Frontiers in Veterinary Science",
title = "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1232570",
volume = "10",
doi = "10.3389/fvets.2023.1232570"
}

Štrbac, F., Krnjajić, S., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Rinaldi, L., Ciccone, E., Maurelli, M. P., Cringoli, G.,& Bosco, A.. (2023). In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science
Frontiers., 10(1232570).
https://doi.org/10.3389/fvets.2023.1232570

Štrbac F, Krnjajić S, Stojanović D, Ratajac R, Simin N, Orčić D, Rinaldi L, Ciccone E, Maurelli MP, Cringoli G, Bosco A. In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science. 2023;10(1232570).
doi:10.3389/fvets.2023.1232570 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Rinaldi, Laura, Ciccone, Elena, Maurelli, Maria Paola, Cringoli, Giuseppe, Bosco, Antonio, "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Frontiers in Veterinary Science, 10, no. 1232570 (2023),
https://doi.org/10.3389/fvets.2023.1232570 . .

TY  - CHAP
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Novakov, Nikolina
AU  - Bosco, Antonio
AU  - Simin, Nataša
AU  - Ratajac, Radomir
AU  - Stanković, Slađan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1899
AB  - Phytotherapy may be defined as the use of plants for the treatment of ailments and those represent a practice that dates since ancient times. It refers to the use of whole plants, their parts such as flowers, leaves, roots and seeds as well as substances extracted from them (plant extracts and essential oils) for treating various diseases. It also may imply their use to support traditional treatment with commercial drugs. Plants and their extracts are an important part of pharmacopoeia in less developed parts of the world, but more recently in the advancement societies. However, plant-based products may also be used for the treatment of diseases in animals, prevalently in livestock. Ethnopharmacology may be implied in veterinary medicine due to the potential therapeutic efficacy, reduced susceptibility to microbial and parasitic resistance, as well as lowered risk of adverse effects and decreased residues in animal products and environment in comparison with chemotherapeutic agents. Moreover, botanical control of various diseases in animals can also be sustainable from the financial point of view. Therefore, medicinal plants are a valuable part of the field of drug discovery and represent an important source of new drugs and drug leads. In this regard, antiparasitic properties are a common point of focus in studies aimed to validate the pharmacological effects of herbal products. A huge number of such plants and their products are considered suitable for the treatment of almost every parasitic disease in livestock. In pets, there are also an increasing number of such studies in dogs and cats, whereby plants product were proven to be effective against various parasites.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
T2  - One Health Triad
T1  - Botanical Control of Parasites in Veterinary Medicine
EP  - 222
IS  - 31
SP  - 215
VL  - 3
DO  - 10.47278/book.oht/2023.98
ER  - 

@inbook{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Novakov, Nikolina and Bosco, Antonio and Simin, Nataša and Ratajac, Radomir and Stanković, Slađan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2023",
abstract = "Phytotherapy may be defined as the use of plants for the treatment of ailments and those represent a practice that dates since ancient times. It refers to the use of whole plants, their parts such as flowers, leaves, roots and seeds as well as substances extracted from them (plant extracts and essential oils) for treating various diseases. It also may imply their use to support traditional treatment with commercial drugs. Plants and their extracts are an important part of pharmacopoeia in less developed parts of the world, but more recently in the advancement societies. However, plant-based products may also be used for the treatment of diseases in animals, prevalently in livestock. Ethnopharmacology may be implied in veterinary medicine due to the potential therapeutic efficacy, reduced susceptibility to microbial and parasitic resistance, as well as lowered risk of adverse effects and decreased residues in animal products and environment in comparison with chemotherapeutic agents. Moreover, botanical control of various diseases in animals can also be sustainable from the financial point of view. Therefore, medicinal plants are a valuable part of the field of drug discovery and represent an important source of new drugs and drug leads. In this regard, antiparasitic properties are a common point of focus in studies aimed to validate the pharmacological effects of herbal products. A huge number of such plants and their products are considered suitable for the treatment of almost every parasitic disease in livestock. In pets, there are also an increasing number of such studies in dogs and cats, whereby plants product were proven to be effective against various parasites.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "One Health Triad",
booktitle = "Botanical Control of Parasites in Veterinary Medicine",
pages = "222-215",
number = "31",
volume = "3",
doi = "10.47278/book.oht/2023.98"
}

Štrbac, F., Krnjajić, S., Stojanović, D., Novakov, N., Bosco, A., Simin, N., Ratajac, R., Stanković, S., Cringoli, G.,& Rinaldi, L.. (2023). Botanical Control of Parasites in Veterinary Medicine. in One Health Triad
Unique Scientific Publishers, Faisalabad, Pakistan., 3(31), 215-222.
https://doi.org/10.47278/book.oht/2023.98

Štrbac F, Krnjajić S, Stojanović D, Novakov N, Bosco A, Simin N, Ratajac R, Stanković S, Cringoli G, Rinaldi L. Botanical Control of Parasites in Veterinary Medicine. in One Health Triad. 2023;3(31):215-222.
doi:10.47278/book.oht/2023.98 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Novakov, Nikolina, Bosco, Antonio, Simin, Nataša, Ratajac, Radomir, Stanković, Slađan, Cringoli, Giuseppe, Rinaldi, Laura, "Botanical Control of Parasites in Veterinary Medicine" in One Health Triad, 3, no. 31 (2023):215-222,
https://doi.org/10.47278/book.oht/2023.98 . .

TY  - CONF
AU  - Saljnikov, Elmira
AU  - Grujić, Tara
AU  - Jovković, Marina
AU  - Stanković, Slađan
AU  - Krnjajić, Slobodan
AU  - Marjanović, Žaklina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2466
AB  - Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. Plastic pollutants in the soil environment have an impact on the cycling of organic matter, global СО2 production, crop production, and soil properties. Crop production in plastic greenhouses is one of the serious sources of plastic pollution in the environment all over the world and particularly in Serbia. One of the
first investigations of plastic and microplastic materials in the soil on the territory of Serbia is currently underway and is being carried out within the project „Evaluation of the Microplastic in the Soils of Serbia – EMIPLAST – SoS“ funded by the Science Fund of the Republic of Serbia. The aim of the research was to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. Soil sampling was carried out in 2022 from two depths (0–15 cm and 15–30 cm), side by side from the plastic greenhouse and non-greenhouse agricultural production as a control in the first project year. The research sites are located on the alluvial plains of the two largest rivers in Serbia, the Danube and the Sava. The following chemical parameters were determined on all samples: electrical conductivity, pH, CEC, total C, as well as nutritional status of the soil (N, P2O5, K2O, Cu, Zn, Mn and CaCO3). Concentrations of some microelements were also analyzed. Physical parameters determined were: particle size distribution, volumetric mass, specific mass and porosity. Microbial respiration, which serves as an indicator of enzymatic microbial activity in the soil, was measured by the alkaline trap method from surface soil.
C3  - The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”
T1  - Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)
EP  - 59
SP  - 58
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2466
ER  - 

@conference{
author = "Saljnikov, Elmira and Grujić, Tara and Jovković, Marina and Stanković, Slađan and Krnjajić, Slobodan and Marjanović, Žaklina",
year = "2023",
abstract = "Plastic pollution is fast becoming a serious global environmental problem with the increase in plastic waste over recent decades. Plastic pollutants in the soil environment have an impact on the cycling of organic matter, global СО2 production, crop production, and soil properties. Crop production in plastic greenhouses is one of the serious sources of plastic pollution in the environment all over the world and particularly in Serbia. One of the
first investigations of plastic and microplastic materials in the soil on the territory of Serbia is currently underway and is being carried out within the project „Evaluation of the Microplastic in the Soils of Serbia – EMIPLAST – SoS“ funded by the Science Fund of the Republic of Serbia. The aim of the research was to reveal the impact of the presence of plastic materials on soil’s main chemical, physical and biological properties. Soil sampling was carried out in 2022 from two depths (0–15 cm and 15–30 cm), side by side from the plastic greenhouse and non-greenhouse agricultural production as a control in the first project year. The research sites are located on the alluvial plains of the two largest rivers in Serbia, the Danube and the Sava. The following chemical parameters were determined on all samples: electrical conductivity, pH, CEC, total C, as well as nutritional status of the soil (N, P2O5, K2O, Cu, Zn, Mn and CaCO3). Concentrations of some microelements were also analyzed. Physical parameters determined were: particle size distribution, volumetric mass, specific mass and porosity. Microbial respiration, which serves as an indicator of enzymatic microbial activity in the soil, was measured by the alkaline trap method from surface soil.",
journal = "The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”",
title = "Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)",
pages = "59-58",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2466"
}

Saljnikov, E., Grujić, T., Jovković, M., Stanković, S., Krnjajić, S.,& Marjanović, Ž.. (2023). Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia). in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”, 58-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2466

Saljnikov E, Grujić T, Jovković M, Stanković S, Krnjajić S, Marjanović Ž. Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia). in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World”. 2023;:58-59.
https://hdl.handle.net/21.15107/rcub_rimsi_2466 .

Saljnikov, Elmira, Grujić, Tara, Jovković, Marina, Stanković, Slađan, Krnjajić, Slobodan, Marjanović, Žaklina, "Impact of plastic pollution on the quality of arable soils in the Sava and Danube river valleys (Serbia)" in The 5th WASWAC World Conference “Adaptation Strategies for Soil and Water Conservation in a Changing World” (2023):58-59,
https://hdl.handle.net/21.15107/rcub_rimsi_2466 .

TY  - GEN
AU  - Popović Milovanović, Tatjana
AU  - Milićević, Zoran
AU  - Krnjajić, Slobodan
AU  - Milovanović, Predrag
AU  - Iličić, Renata
AU  - Zečević, Katarina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2070
AB  - U okviru ove studije, prvi je put u svetu uspešno sintetizovana nova stabilna i homogena EC formulacije ulja karanfilića (S. aromaticum) zasnovana na biopolimerima kao što je zeolit. Nova formulacija ulja karanfilića (F-CSZ) prema fizičkim svojstvima ispunjava kriterijume za EC formulaciju u obliku kojem je sintetizovana. Novoformulisano, inkapsulirano ulje karanfilića obezbeđuje ekološki prihvatljivu zamenu sintetičkim pesticidima u kontroli štetočina na osnovu ispoljavanja visoke biološke efikasnosti u kontroli brojnosti imaga krompirovog moljca ali i  bezbeđivanjem produžene efikasnosti ulja do 14 dana u odnosu na čisto etarsko ulje karanfilića koje je izgubilo insekticidnu aktivnost nakon 24 h od tretmana. Nova formulacija ulja karanfilića (F-CSZ) je ispoljila visoku efikasnost u suzbijanju imaga krompirovog moljca, a da pri tome nije uticala na kvalitet krompira te se može komercijalno  koristiti kao fumigant ili zaštitni premaz za suzbijanje štetočina u skladištu, a samim tim i za smanjenje gubitaka tokom skladištenja i čuvanja proizvoda.
PB  - Министарство науке, технолошког развоја и иновација Републике Србије; МНО за биотехнологију и пољопривреду
T2  - Institut za zaštitu bilja i životnu sredinu, Beograd; Univerzitet u Beogradu - Institut za multidisciplinarna istraživanja; Univerzitet u Novom Sadu, Poljoprivredni fakultet; Univerzitet u Beogradu, Poljoprivredni fakultet
T1  - Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella)
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2070
ER  - 

@misc{
author = "Popović Milovanović, Tatjana and Milićević, Zoran and Krnjajić, Slobodan and Milovanović, Predrag and Iličić, Renata and Zečević, Katarina",
year = "2023",
abstract = "U okviru ove studije, prvi je put u svetu uspešno sintetizovana nova stabilna i homogena EC formulacije ulja karanfilića (S. aromaticum) zasnovana na biopolimerima kao što je zeolit. Nova formulacija ulja karanfilića (F-CSZ) prema fizičkim svojstvima ispunjava kriterijume za EC formulaciju u obliku kojem je sintetizovana. Novoformulisano, inkapsulirano ulje karanfilića obezbeđuje ekološki prihvatljivu zamenu sintetičkim pesticidima u kontroli štetočina na osnovu ispoljavanja visoke biološke efikasnosti u kontroli brojnosti imaga krompirovog moljca ali i  bezbeđivanjem produžene efikasnosti ulja do 14 dana u odnosu na čisto etarsko ulje karanfilića koje je izgubilo insekticidnu aktivnost nakon 24 h od tretmana. Nova formulacija ulja karanfilića (F-CSZ) je ispoljila visoku efikasnost u suzbijanju imaga krompirovog moljca, a da pri tome nije uticala na kvalitet krompira te se može komercijalno  koristiti kao fumigant ili zaštitni premaz za suzbijanje štetočina u skladištu, a samim tim i za smanjenje gubitaka tokom skladištenja i čuvanja proizvoda.",
publisher = "Министарство науке, технолошког развоја и иновација Републике Србије; МНО за биотехнологију и пољопривреду",
journal = "Institut za zaštitu bilja i životnu sredinu, Beograd; Univerzitet u Beogradu - Institut za multidisciplinarna istraživanja; Univerzitet u Novom Sadu, Poljoprivredni fakultet; Univerzitet u Beogradu, Poljoprivredni fakultet",
title = "Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella)",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2070"
}

Popović Milovanović, T., Milićević, Z., Krnjajić, S., Milovanović, P., Iličić, R.,& Zečević, K.. (2023). Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella). in Institut za zaštitu bilja i životnu sredinu, Beograd; Univerzitet u Beogradu - Institut za multidisciplinarna istraživanja; Univerzitet u Novom Sadu, Poljoprivredni fakultet; Univerzitet u Beogradu, Poljoprivredni fakultet
Министарство науке, технолошког развоја и иновација Републике Србије; МНО за биотехнологију и пољопривреду..
https://hdl.handle.net/21.15107/rcub_rimsi_2070

Popović Milovanović T, Milićević Z, Krnjajić S, Milovanović P, Iličić R, Zečević K. Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella). in Institut za zaštitu bilja i životnu sredinu, Beograd; Univerzitet u Beogradu - Institut za multidisciplinarna istraživanja; Univerzitet u Novom Sadu, Poljoprivredni fakultet; Univerzitet u Beogradu, Poljoprivredni fakultet. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2070 .

Popović Milovanović, Tatjana, Milićević, Zoran, Krnjajić, Slobodan, Milovanović, Predrag, Iličić, Renata, Zečević, Katarina, "Nova formulacija ulja karanfilića (Syzygium aromaticum L.) u kontroli brojnosti krompirovog moljca (Phthorimaea operculella)" in Institut za zaštitu bilja i životnu sredinu, Beograd; Univerzitet u Beogradu - Institut za multidisciplinarna istraživanja; Univerzitet u Novom Sadu, Poljoprivredni fakultet; Univerzitet u Beogradu, Poljoprivredni fakultet (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2070 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Knežević, Goran
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Bosco, Antonio
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1884
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.
PB  - MDPI
T2  - Animals
T1  - A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1
SP  - 45
VL  - 13
DO  - 10.3390/ani13010045
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Knežević, Goran and Cringoli, Giuseppe and Rinaldi, Laura and Bosco, Antonio",
year = "2022",
abstract = "The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.",
publisher = "MDPI",
journal = "Animals",
title = "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1",
pages = "45",
volume = "13",
doi = "10.3390/ani13010045"
}

Štrbac, F., Krnjajić, S., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Knežević, G., Cringoli, G., Rinaldi, L.,& Bosco, A.. (2022). A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals
MDPI., 13(1), 45.
https://doi.org/10.3390/ani13010045

Štrbac F, Krnjajić S, Maurelli MP, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Knežević G, Cringoli G, Rinaldi L, Bosco A. A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals. 2022;13(1):45.
doi:10.3390/ani13010045 .

Štrbac, Filip, Krnjajić, Slobodan, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Knežević, Goran, Cringoli, Giuseppe, Rinaldi, Laura, Bosco, Antonio, "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Animals, 13, no. 1 (2022):45,
https://doi.org/10.3390/ani13010045 . .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Maurelli, Maria Paola
AU  - Ratajac, Radomir
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orcic, Dejan
AU  - Pusic, Ivan
AU  - Krnjajić, Slobodan
AU  - Sotiraki, Smaragda
AU  - Saralli, Giorgio
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1548
AB  - Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.
PB  - MDPI, Basel
T2  - Veterinary Sciences
T1  - Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies
IS  - 2
VL  - 9
DO  - 10.3390/vetsci9020093
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Maurelli, Maria Paola and Ratajac, Radomir and Stojanović, Dragica and Simin, Nataša and Orcic, Dejan and Pusic, Ivan and Krnjajić, Slobodan and Sotiraki, Smaragda and Saralli, Giorgio and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.",
publisher = "MDPI, Basel",
journal = "Veterinary Sciences",
title = "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies",
number = "2",
volume = "9",
doi = "10.3390/vetsci9020093"
}

Štrbac, F., Bosco, A., Maurelli, M. P., Ratajac, R., Stojanović, D., Simin, N., Orcic, D., Pusic, I., Krnjajić, S., Sotiraki, S., Saralli, G., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences
MDPI, Basel., 9(2).
https://doi.org/10.3390/vetsci9020093

Štrbac F, Bosco A, Maurelli MP, Ratajac R, Stojanović D, Simin N, Orcic D, Pusic I, Krnjajić S, Sotiraki S, Saralli G, Cringoli G, Rinaldi L. Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences. 2022;9(2).
doi:10.3390/vetsci9020093 .

Štrbac, Filip, Bosco, Antonio, Maurelli, Maria Paola, Ratajac, Radomir, Stojanović, Dragica, Simin, Nataša, Orcic, Dejan, Pusic, Ivan, Krnjajić, Slobodan, Sotiraki, Smaragda, Saralli, Giorgio, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies" in Veterinary Sciences, 9, no. 2 (2022),
https://doi.org/10.3390/vetsci9020093 . .

TY  - JOUR
AU  - Milićević, Z.
AU  - Krnjajić, Slobodan
AU  - Stević, M.
AU  - Ćirković, Jovana
AU  - Jelušić, Aleksandra
AU  - Pucarević, M.
AU  - Popović, Tatjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1549
AB  - In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.
PB  - MDPI
T2  - Agriculture (Switzerland)
T1  - Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide
IS  - 3
VL  - 12
DO  - 10.3390/agriculture12030338
ER  - 

@article{
author = "Milićević, Z. and Krnjajić, Slobodan and Stević, M. and Ćirković, Jovana and Jelušić, Aleksandra and Pucarević, M. and Popović, Tatjana",
year = "2022",
abstract = "In this work by encapsulation technique we have synthetized three new clove bud essential oil (CEO) Emulsifiable Concentrate (EC) formulations depending on the carrier (synthetic zeolite-F-CSZ, nature zeolite-F-CNZ and gelatin-F-CG). The main idea was to develop an eco-friendly biopesticide that can find use in plant protection as an alternative to the use of conventional pesticides. By encapsulation we wanted to enable water solubility and ensure prolonged efficacy of the essential oil. Biological activity of designed CEO formulations was tested on potato tuber moth Phthorimaea operculella (fumigant mode of action), gray mold fungal pathogen Botrytis cinerea (preserver coatings), and soft rotting bacterial pathogens Pectobacterium carotovorum (subsp. carotovorum and brasiliensis) and Dickeya dianthicola (direct competition). CEO formulations evinced a prolonged action on mortality of P. operculella during the insects’ exposure to the concentration of the emulsions of 40 µL L−1 air. The mortality gradually decreased from a probability of 100% after the first 24 h to 50% after 5 days for F-CSZ or after 4 days for F-CNZ and F-CG. The most promising formulation is F-CSZ enabling activity during 14 days of exposure, while the effect of the other two formulations lasted 10 days. All three formulations produced a strong fungicidal effect against B. cinerea by preventing infection and disease development. The best efficacy was evidenced with F-CSZ (synthetic zeolite as a carrier) showing 100% efficacy when it was used even at the lowest tested concentration of active CEO (1%). The results of in vitro testing against soft rot pathogens determined the MIC value of CEO formulations to be 1% of active CEO. By this research, we present a novel perspective on the use of essential oils as an alternative, environmental biopesticide. CEO formulations can be commercially exploited as a fumigant or preserver coatings to extend the shelf life of stored products or the fresh-fruit market.",
publisher = "MDPI",
journal = "Agriculture (Switzerland)",
title = "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide",
number = "3",
volume = "12",
doi = "10.3390/agriculture12030338"
}

Milićević, Z., Krnjajić, S., Stević, M., Ćirković, J., Jelušić, A., Pucarević, M.,& Popović, T.. (2022). Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland)
MDPI., 12(3).
https://doi.org/10.3390/agriculture12030338

Milićević Z, Krnjajić S, Stević M, Ćirković J, Jelušić A, Pucarević M, Popović T. Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide. in Agriculture (Switzerland). 2022;12(3).
doi:10.3390/agriculture12030338 .

Milićević, Z., Krnjajić, Slobodan, Stević, M., Ćirković, Jovana, Jelušić, Aleksandra, Pucarević, M., Popović, Tatjana, "Encapsulated Clove Bud Essential Oil: A New Perspective as an Eco-Friendly Biopesticide" in Agriculture (Switzerland), 12, no. 3 (2022),
https://doi.org/10.3390/agriculture12030338 . .

TY  - CONF
AU  - Lucev, Milica
AU  - Savic, Iva
AU  - Radotić, Ksenija
AU  - Krnjajić, Slobodan
AU  - Bartolić, Dragana
AU  - Stevanovic, Milan
AU  - Stanković, Slavica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2866
AB  - The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus
PB  - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
C3  - Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
T1  - ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS
EP  - 362
SP  - 362
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2866
ER  - 

@conference{
author = "Lucev, Milica and Savic, Iva and Radotić, Ksenija and Krnjajić, Slobodan and Bartolić, Dragana and Stevanovic, Milan and Stanković, Slavica",
year = "2021",
abstract = "The application of pesticides is an actual plant protection measure in agriculture that can have
adverse effects on people’s health and environments. A great attention is paid to the biological
fungicides. Performed studies indicate satisfactory results of activities of essential oils, which
furthermore point out to possibilities of their inclusion in crop protection programs. The aim of
this study was to determine antifungal, contact activity of essential oils of thyme (Thymus vulgaris
L.) and oregano (Origanum vulgare L.) on toxigenic fungal species Aspergillus flavus causing
maize kernel rot. The sterile filter paper was placed in the inner lead of Petri dish. Two, four, six,
eight and 10 μl of essential oils were pipetted on the paper, and then pure A. flavus cultures were
subcultured on PDA. After seven days in the dark at 25°C, the degree of inhibition was
determined by measuring the fungal growth and their comparison to the control. The fungus,
without addition of essential oils, was used as the control. The strongest antifungal activity was
expressed by thyme essential oil, which already at the amount of 2 μl completely inhibited the
mycelium growth. The identical effect was achieved with the amount of 4 μl. The greater amount
of essential oil the more progressive growth of the fungal colony (6 μl – 3 mm; 8 μl – 9 mm).
However, the mycelium growth at the amount of 10 μl of essential oil was only 1 mm. The
similar results were gained with oregano essential oil (2 μl – 3 mm; 4 μl – 7 mm; 6 μl – 9 mm; 8
μl – 9 mm; 10 – 6 mm). The fungal growth of control was 30 mm. The obtained results indicate
the significant potential of the application of thyme and oregano essential oils as possible natural
and environmentally friendly means for the protection of maize against A. flavus",
publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia",
journal = "Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"",
title = "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS",
pages = "362-362",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2866"
}

Lucev, M., Savic, I., Radotić, K., Krnjajić, S., Bartolić, D., Stevanovic, M.,& Stanković, S.. (2021). ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021"
University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866

Lucev M, Savic I, Radotić K, Krnjajić S, Bartolić D, Stevanovic M, Stanković S. ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS. in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021". 2021;:362-362.
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .

Lucev, Milica, Savic, Iva, Radotić, Ksenija, Krnjajić, Slobodan, Bartolić, Dragana, Stevanovic, Milan, Stanković, Slavica, "ANTIFUNGAL ACTIVITY OF ESSENTIAL OILS ON ASPERGILLUS FLAVUS ORIGINATING FROM MAIZE KERNELS" in Book of Abstracts of the XII International Scientific Agriculture Symposium "Agrosym 2021" (2021):362-362,
https://hdl.handle.net/21.15107/rcub_rimsi_2866 .

TY  - CONF
AU  - Knežević, Goran
AU  - Radović, Vesela
AU  - Krnjajić, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2719
AB  - Sustainable rural development is vital to the economic and environmental
viability of the Serbia. Serbian rural population relies on agriculture for their
livelihoods. Making agriculture in rural areas sustainable and competitive is
not an easy to achieve. Authors highlighted the new challenge for this goal.
The European Commission withdraws chemicals for preventing sprouting of
potato (Commission Implementing Regulation (EU) 2019/989). Faced with
this demand, potato producers have to find a way how to stay competitive on
a current market, and do not additionally harm already fragile environmental
state. Authors in this article propose the use of essential oils for prevention
the potato sprouting. Exploring different kind of essential oils to solve this
problem, researchers obtained useful results. There is a new option to encourage
use of essential oils in potato production. So, producers will avoid new
significant production costs, stay in the competitive race to the production
line, and enhance sustainable development of rural areas.
PB  - Institute of Agricultural Economics, Belgrade, Serbia
C3  - SUSTAINABLE AGRICULTURE AND RURAL DEVELOPMENT II BOOK OF ABSTRACTS
T1  - APPLICATION OF INNOVATIVE TECHNOLOGIES IN AGRICULTURE AS A PART OF THE SUSTAINABLE DEVELOPMENT OF SERBIAN RURAL AREAS
EP  - 31
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2719
ER  - 

@conference{
author = "Knežević, Goran and Radović, Vesela and Krnjajić, Slobodan",
year = "2021",
abstract = "Sustainable rural development is vital to the economic and environmental
viability of the Serbia. Serbian rural population relies on agriculture for their
livelihoods. Making agriculture in rural areas sustainable and competitive is
not an easy to achieve. Authors highlighted the new challenge for this goal.
The European Commission withdraws chemicals for preventing sprouting of
potato (Commission Implementing Regulation (EU) 2019/989). Faced with
this demand, potato producers have to find a way how to stay competitive on
a current market, and do not additionally harm already fragile environmental
state. Authors in this article propose the use of essential oils for prevention
the potato sprouting. Exploring different kind of essential oils to solve this
problem, researchers obtained useful results. There is a new option to encourage
use of essential oils in potato production. So, producers will avoid new
significant production costs, stay in the competitive race to the production
line, and enhance sustainable development of rural areas.",
publisher = "Institute of Agricultural Economics, Belgrade, Serbia",
journal = "SUSTAINABLE AGRICULTURE AND RURAL DEVELOPMENT II BOOK OF ABSTRACTS",
title = "APPLICATION OF INNOVATIVE TECHNOLOGIES IN AGRICULTURE AS A PART OF THE SUSTAINABLE DEVELOPMENT OF SERBIAN RURAL AREAS",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2719"
}

Knežević, G., Radović, V.,& Krnjajić, S.. (2021). APPLICATION OF INNOVATIVE TECHNOLOGIES IN AGRICULTURE AS A PART OF THE SUSTAINABLE DEVELOPMENT OF SERBIAN RURAL AREAS. in SUSTAINABLE AGRICULTURE AND RURAL DEVELOPMENT II BOOK OF ABSTRACTS
Institute of Agricultural Economics, Belgrade, Serbia., 31-31.
https://hdl.handle.net/21.15107/rcub_rimsi_2719

Knežević G, Radović V, Krnjajić S. APPLICATION OF INNOVATIVE TECHNOLOGIES IN AGRICULTURE AS A PART OF THE SUSTAINABLE DEVELOPMENT OF SERBIAN RURAL AREAS. in SUSTAINABLE AGRICULTURE AND RURAL DEVELOPMENT II BOOK OF ABSTRACTS. 2021;:31-31.
https://hdl.handle.net/21.15107/rcub_rimsi_2719 .

Knežević, Goran, Radović, Vesela, Krnjajić, Slobodan, "APPLICATION OF INNOVATIVE TECHNOLOGIES IN AGRICULTURE AS A PART OF THE SUSTAINABLE DEVELOPMENT OF SERBIAN RURAL AREAS" in SUSTAINABLE AGRICULTURE AND RURAL DEVELOPMENT II BOOK OF ABSTRACTS (2021):31-31,
https://hdl.handle.net/21.15107/rcub_rimsi_2719 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1972
AB  - Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.
PB  - World Association for the Advancement of Veterinary Parasitology (WAAVP)
C3  - Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
T1  - In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes
EP  - 517
SP  - 517
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1972
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.",
publisher = "World Association for the Advancement of Veterinary Parasitology (WAAVP)",
journal = "Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland",
title = "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes",
pages = "517-517",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1972"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
World Association for the Advancement of Veterinary Parasitology (WAAVP)., 517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland. 2021;:517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes" in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland (2021):517-517,
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1975
AB  - Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
T1  - Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties
EP  - 161
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1975
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"",
title = "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties",
pages = "161-160",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1975"
}

Štrbac, F., Simin, N., Orčić, D., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975

Štrbac F, Simin N, Orčić D, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Pušić I, Krnjajić S, Ratajac R. Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms". 2021;:160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties" in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms" (2021):160-161,
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Mangieri, Giuseppe
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1888
AB  - Based on the results in different studies conducted so far, plant essential oils (EOs) are recently been marked as a possible solution for the problem of anthelmintic resistance (AR). However, their efficacy against a wide range of livestock parasites is due to active ingredients. In that context, the aim of this study was to evaluate the in vitro ovicidal effect of a mixture of linalool and estragole (14% : 86%, respectively) against sheep gastrointestinal nematodes (GINs) using the egg hatch test (EHT). The study was conducted using faecal samples of naturally infected sheep by GINs from two different farms located in Southern Italy. Coproculture of tested faecal samples was performed, as well as chemical analyses of the tested mixture. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) and the obtained values were compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (3% Tween 80, v/v). The tested binary combination showed high and dose-dependent ovicidal activity varied from 29.5% to 100%. The inhibitory effect on egg hatchability was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (100%), 12.5 mg/mL (100%) and 3.125 (99.8%). Moreover, all tested concentrations showed significantly higher (p<0.001) activity compared to the negative control. The obtained results suggest that the combination of linalool:estragole has the potential to be used against sheep GINs. These also highlight the possible role of different combinations of certain constituents of essential oils in combating AR. However, these findings need confirmation in further in vivo studies.
AB  - Na osnovu rezultata do sada sprovedenih istraživanja, etarska ulja biljaka su označena kao jedna od mogućih opcija za rešenje problemarezistencije na antihelmintike. Pri tome, za njihovu efikasnost protiv različitih parazita domaćih životinja odgovorni su njihovi aktivni sastojci. U tom kontekstu, cilj ovog istraživanja je bio ispitati ovicidnu efikasnost smeše linaloola i estragola (14% i 86%, tim redom) protiv gastrointestinalnih nematoda (GIN) ovaca koristeći test izleganja jaja (egg hatch test (EHT)). U studiji su korišćeni uzorci fecesa ovaca prirodno inficiranih GIN sa dve različite farme u južnoj Italiji. Koprokultura testiranih uzoraka je sprovedena, kao i hemijske analize testirane smeše. EHT je sproveden za šest različitih koncentracija (50; 12,5; 3,125; 0,781; 0,195 i 0,049 mg/mL), pri čemu su dobijeni rezultati upoređeni sa pozitivnom (tiabendazol, 0.025 mg/mL) i negativnom (3% Tween 80) kontrolom. Testirana binarna kombinacija je pokazala snažnu, dozno-zavisnu ovicidnu aktivnost koja je varirala između 29,5% i 100%. Inhibitorni efekat na izleganje larvi je bio sličan (p<0,05) pozitivnoj kontroli (98,0%) pri koncentracijama od 50 mg/mL (100%), 12,5 mg/mL (100%) i 3,125 mg/mL (99,8%). Pri tome, kod svih testiranih koncentracija efekat je bio značajno veći (p<0,001) u poređenju sa negativnom kontrolom. Dobijeni rezultati ukazuju na to da ispitana smeša linaloola i estragola ima potencijal da se koristi protiv GIN ovaca. Takođe, ova studija ukazuje na moguću ulogu različitih sastojaka biljnih etarskih ulja u borbi protiv rezistencije na antihelmintike. Međutim, ovi nalazi zahtevaju dodatnu potvrdu u budućim in vivo ispitivanjima.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep
T1  - In vitro ovicidna aktivnost smeše linaloola i estragola protiv gastrointestinalnih nematoda ovaca
EP  - 59
IS  - 1
SP  - 49
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1888
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Mangieri, Giuseppe and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Based on the results in different studies conducted so far, plant essential oils (EOs) are recently been marked as a possible solution for the problem of anthelmintic resistance (AR). However, their efficacy against a wide range of livestock parasites is due to active ingredients. In that context, the aim of this study was to evaluate the in vitro ovicidal effect of a mixture of linalool and estragole (14% : 86%, respectively) against sheep gastrointestinal nematodes (GINs) using the egg hatch test (EHT). The study was conducted using faecal samples of naturally infected sheep by GINs from two different farms located in Southern Italy. Coproculture of tested faecal samples was performed, as well as chemical analyses of the tested mixture. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) and the obtained values were compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (3% Tween 80, v/v). The tested binary combination showed high and dose-dependent ovicidal activity varied from 29.5% to 100%. The inhibitory effect on egg hatchability was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (100%), 12.5 mg/mL (100%) and 3.125 (99.8%). Moreover, all tested concentrations showed significantly higher (p<0.001) activity compared to the negative control. The obtained results suggest that the combination of linalool:estragole has the potential to be used against sheep GINs. These also highlight the possible role of different combinations of certain constituents of essential oils in combating AR. However, these findings need confirmation in further in vivo studies., Na osnovu rezultata do sada sprovedenih istraživanja, etarska ulja biljaka su označena kao jedna od mogućih opcija za rešenje problemarezistencije na antihelmintike. Pri tome, za njihovu efikasnost protiv različitih parazita domaćih životinja odgovorni su njihovi aktivni sastojci. U tom kontekstu, cilj ovog istraživanja je bio ispitati ovicidnu efikasnost smeše linaloola i estragola (14% i 86%, tim redom) protiv gastrointestinalnih nematoda (GIN) ovaca koristeći test izleganja jaja (egg hatch test (EHT)). U studiji su korišćeni uzorci fecesa ovaca prirodno inficiranih GIN sa dve različite farme u južnoj Italiji. Koprokultura testiranih uzoraka je sprovedena, kao i hemijske analize testirane smeše. EHT je sproveden za šest različitih koncentracija (50; 12,5; 3,125; 0,781; 0,195 i 0,049 mg/mL), pri čemu su dobijeni rezultati upoređeni sa pozitivnom (tiabendazol, 0.025 mg/mL) i negativnom (3% Tween 80) kontrolom. Testirana binarna kombinacija je pokazala snažnu, dozno-zavisnu ovicidnu aktivnost koja je varirala između 29,5% i 100%. Inhibitorni efekat na izleganje larvi je bio sličan (p<0,05) pozitivnoj kontroli (98,0%) pri koncentracijama od 50 mg/mL (100%), 12,5 mg/mL (100%) i 3,125 mg/mL (99,8%). Pri tome, kod svih testiranih koncentracija efekat je bio značajno veći (p<0,001) u poređenju sa negativnom kontrolom. Dobijeni rezultati ukazuju na to da ispitana smeša linaloola i estragola ima potencijal da se koristi protiv GIN ovaca. Takođe, ova studija ukazuje na moguću ulogu različitih sastojaka biljnih etarskih ulja u borbi protiv rezistencije na antihelmintike. Međutim, ovi nalazi zahtevaju dodatnu potvrdu u budućim in vivo ispitivanjima.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep, In vitro ovicidna aktivnost smeše linaloola i estragola protiv gastrointestinalnih nematoda ovaca",
pages = "59-49",
number = "1",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1888"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Mangieri, G., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 2(1), 49-59.
https://hdl.handle.net/21.15107/rcub_rimsi_1888

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Mangieri G, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep. in Veterinarski Pregled. 2021;2(1):49-59.
https://hdl.handle.net/21.15107/rcub_rimsi_1888 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Mangieri, Giuseppe, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep" in Veterinarski Pregled, 2, no. 1 (2021):49-59,
https://hdl.handle.net/21.15107/rcub_rimsi_1888 .

TY  - CONF
AU  - Oro, Violeta
AU  - Popović, Tatjana
AU  - Krnjajić, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2660
AB  - Essential oils from citrus plants are getting increasing interest in the agricultural pest
management. Numerous studies demonstrate their antibacterial, antifungal, insecticidal
and nematicidal properties. This study was conducted to determine the activity of the
essential oils from citrus plants (bergamot, bitter orange, grapefruit, mandarin, orange, and
lemon) against the rhabditid nematode Panagrolaimus sp. In vitro nematicide screening was
performed with aqueous solutions of essential oils in serial dilutions (1-0.0078%). The contact
bioassay was done in small glass Petri dishes containing 3 ml of solution and 50 nematodes
at 18oC. The experiment was carried out in five replicates. The lethal effect was monitored
after 24h. Median lethal concentration (LC50) was calculated using the Probit analysis. The
best nematicidal activity performed the essential oil from lemon, followed by bergamot.
The bitter orange had better nematicidal properties than mandarin and grapefruit essential
oils. The least activity demonstrated orange oil.
PB  - Plant Protection Society of Serbia
C3  - Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS
T1  - Nematicidal investigations of some essential oils from citrus plants
EP  - 142
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2660
ER  - 

@conference{
author = "Oro, Violeta and Popović, Tatjana and Krnjajić, Slobodan",
year = "2021",
abstract = "Essential oils from citrus plants are getting increasing interest in the agricultural pest
management. Numerous studies demonstrate their antibacterial, antifungal, insecticidal
and nematicidal properties. This study was conducted to determine the activity of the
essential oils from citrus plants (bergamot, bitter orange, grapefruit, mandarin, orange, and
lemon) against the rhabditid nematode Panagrolaimus sp. In vitro nematicide screening was
performed with aqueous solutions of essential oils in serial dilutions (1-0.0078%). The contact
bioassay was done in small glass Petri dishes containing 3 ml of solution and 50 nematodes
at 18oC. The experiment was carried out in five replicates. The lethal effect was monitored
after 24h. Median lethal concentration (LC50) was calculated using the Probit analysis. The
best nematicidal activity performed the essential oil from lemon, followed by bergamot.
The bitter orange had better nematicidal properties than mandarin and grapefruit essential
oils. The least activity demonstrated orange oil.",
publisher = "Plant Protection Society of Serbia",
journal = "Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS",
title = "Nematicidal investigations of some essential oils from citrus plants",
pages = "142-135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2660"
}

Oro, V., Popović, T.,& Krnjajić, S.. (2021). Nematicidal investigations of some essential oils from citrus plants. in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS
Plant Protection Society of Serbia., 135-142.
https://hdl.handle.net/21.15107/rcub_rimsi_2660

Oro V, Popović T, Krnjajić S. Nematicidal investigations of some essential oils from citrus plants. in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS. 2021;:135-142.
https://hdl.handle.net/21.15107/rcub_rimsi_2660 .

Oro, Violeta, Popović, Tatjana, Krnjajić, Slobodan, "Nematicidal investigations of some essential oils from citrus plants" in Proceedings of the VIII Congress on Plant Protection (November 25-29, 2019, Zlatibor, Serbia). IOBC-WPRS, Plant Protection Society of Serbia and IOBC-EPRS (2021):135-142,
https://hdl.handle.net/21.15107/rcub_rimsi_2660 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Seslija-Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Calic, Dusica D
AU  - Stanković, Sladan
AU  - Kostic, Miroslav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1506
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge Univ Press, Cambridge
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
EP  - 199
IS  - 2
SP  - 190
VL  - 111
DO  - 10.1017/S0007485320000504
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Seslija-Jovanović, Darka and Krnjajić, Slobodan and Calic, Dusica D and Stanković, Sladan and Kostic, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
pages = "199-190",
number = "2",
volume = "111",
doi = "10.1017/S0007485320000504"
}

Lazarević, J., Kostić, I., Milanović, S., Seslija-Jovanović, D., Krnjajić, S., Calic, D. D., Stanković, S.,& Kostic, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge Univ Press, Cambridge., 111(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Seslija-Jovanović D, Krnjajić S, Calic DD, Stanković S, Kostic M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;111(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Seslija-Jovanović, Darka, Krnjajić, Slobodan, Calic, Dusica D, Stanković, Sladan, Kostic, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 111, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - JOUR
AU  - Grbic, Gordana
AU  - Haenggi, Ambros
AU  - Krnjajić, Slobodan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1514
AB  - Good environmental management needs evidence-based conservation measures, and those measures need both faunistical and ecological information. Following this path, for the first time in Serbia, a faunistical research of spiders at Subotica Sandland was organised in 2014 as a base for ecological arguments in landscape management of the area. The spiders were collected at ten different habitats on sandy soil, in the period from 27th April till 30th October by pitfall trapping and sweep netting. A total of 16304 adult and 7246 juvenile individuals were captured, and 225 species from 27 families were determined. Thirty species represent new records for Serbia. Diversity and species compositions provided an insight into the quality of the habitats and the influence of the conservation and development measures that were already applied. The main endangerment factors are outlined. Conclusions and suggestions according to the analysis of the spider fauna, are mostly in correlation with those made earlier based on other groups of organisms. Within the scope of nature protection, wet and sandy meadows are prioritised over the woods. For future monitoring, two flagship and umbrella species are suggested: Argiope lobata (Pallas, 1772) for the sandy area and Dolomedes plantarius (Clerck, 1757) for the wetlands.
PB  - Hungarian Natural History Museum, Budapest
T2  - Acta Zoologica Academiae Scientiarum Hungaricae
T1  - Spiders (araneae) of Subotica sandland (Serbia): additional arguments in environmental protection
EP  - 61
IS  - 1
SP  - 15
VL  - 67
DO  - 10.17109/AZH.67.1.15.2021
ER  - 

@article{
author = "Grbic, Gordana and Haenggi, Ambros and Krnjajić, Slobodan",
year = "2021",
abstract = "Good environmental management needs evidence-based conservation measures, and those measures need both faunistical and ecological information. Following this path, for the first time in Serbia, a faunistical research of spiders at Subotica Sandland was organised in 2014 as a base for ecological arguments in landscape management of the area. The spiders were collected at ten different habitats on sandy soil, in the period from 27th April till 30th October by pitfall trapping and sweep netting. A total of 16304 adult and 7246 juvenile individuals were captured, and 225 species from 27 families were determined. Thirty species represent new records for Serbia. Diversity and species compositions provided an insight into the quality of the habitats and the influence of the conservation and development measures that were already applied. The main endangerment factors are outlined. Conclusions and suggestions according to the analysis of the spider fauna, are mostly in correlation with those made earlier based on other groups of organisms. Within the scope of nature protection, wet and sandy meadows are prioritised over the woods. For future monitoring, two flagship and umbrella species are suggested: Argiope lobata (Pallas, 1772) for the sandy area and Dolomedes plantarius (Clerck, 1757) for the wetlands.",
publisher = "Hungarian Natural History Museum, Budapest",
journal = "Acta Zoologica Academiae Scientiarum Hungaricae",
title = "Spiders (araneae) of Subotica sandland (Serbia): additional arguments in environmental protection",
pages = "61-15",
number = "1",
volume = "67",
doi = "10.17109/AZH.67.1.15.2021"
}

Grbic, G., Haenggi, A.,& Krnjajić, S.. (2021). Spiders (araneae) of Subotica sandland (Serbia): additional arguments in environmental protection. in Acta Zoologica Academiae Scientiarum Hungaricae
Hungarian Natural History Museum, Budapest., 67(1), 15-61.
https://doi.org/10.17109/AZH.67.1.15.2021

Grbic G, Haenggi A, Krnjajić S. Spiders (araneae) of Subotica sandland (Serbia): additional arguments in environmental protection. in Acta Zoologica Academiae Scientiarum Hungaricae. 2021;67(1):15-61.
doi:10.17109/AZH.67.1.15.2021 .

Grbic, Gordana, Haenggi, Ambros, Krnjajić, Slobodan, "Spiders (araneae) of Subotica sandland (Serbia): additional arguments in environmental protection" in Acta Zoologica Academiae Scientiarum Hungaricae, 67, no. 1 (2021):15-61,
https://doi.org/10.17109/AZH.67.1.15.2021 . .

TY  - PAT
AU  - Branković, Zorica
AU  - Ćirković, Jovana
AU  - Radojković, Aleksandar
AU  - Branković, Goran
AU  - Jovanović, Jelena
AU  - Krnjajić, Slobodan
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2720
AB  - The present invention is in the field of aqueous emulsions that dry into water-insoluble or water-resistant structures that
are useful for active packaging, manufactured devices and components, and other applications. The aqueous emulsions of the present invention comprise biopolymers, metal in the form of a salt, nanoparticles or metal oxide nanoparticles, essential oil, and additives such as surfactants and plasticizers. When the components of the emulsion are mixed following the distinctive method of preparation, a water-soluble fluid is obtained, which, upon drying, becomes a water-insoluble or water-resistant solid exhibiting antimicrobial, antioxidative, and other useful properties including tensile strength, elasticity, transparency. The obtained fluid may be applied by spraying, pouring, injecting, 3-D printing, or otherwise formed into a solid product of any geometrical shape including film, foil, or other 3-D shape.
PB  - The World Intellectual Property Organization (WIPO)
T2  - International Patent Application No. PCT/RS2018/000013, International Filling Date: September 14, 2018., Applicants: University of Belgrade, Publication Number: WO 2020/055277 A1, Publication Date: March192020.
T1  - BIOPOLYMER EMULSION FOR ACTIVE PACKAGING, USES AND METHOD OF MANUFACTURING
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2720
ER  - 

@misc{
author = "Branković, Zorica and Ćirković, Jovana and Radojković, Aleksandar and Branković, Goran and Jovanović, Jelena and Krnjajić, Slobodan and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "The present invention is in the field of aqueous emulsions that dry into water-insoluble or water-resistant structures that
are useful for active packaging, manufactured devices and components, and other applications. The aqueous emulsions of the present invention comprise biopolymers, metal in the form of a salt, nanoparticles or metal oxide nanoparticles, essential oil, and additives such as surfactants and plasticizers. When the components of the emulsion are mixed following the distinctive method of preparation, a water-soluble fluid is obtained, which, upon drying, becomes a water-insoluble or water-resistant solid exhibiting antimicrobial, antioxidative, and other useful properties including tensile strength, elasticity, transparency. The obtained fluid may be applied by spraying, pouring, injecting, 3-D printing, or otherwise formed into a solid product of any geometrical shape including film, foil, or other 3-D shape.",
publisher = "The World Intellectual Property Organization (WIPO)",
journal = "International Patent Application No. PCT/RS2018/000013, International Filling Date: September 14, 2018., Applicants: University of Belgrade, Publication Number: WO 2020/055277 A1, Publication Date: March192020.",
title = "BIOPOLYMER EMULSION FOR ACTIVE PACKAGING, USES AND METHOD OF MANUFACTURING",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2720"
}

Branković, Z., Ćirković, J., Radojković, A., Branković, G., Jovanović, J., Krnjajić, S.,& Veljović-Jovanović, S.. (2020). BIOPOLYMER EMULSION FOR ACTIVE PACKAGING, USES AND METHOD OF MANUFACTURING. in International Patent Application No. PCT/RS2018/000013, International Filling Date: September 14, 2018., Applicants: University of Belgrade, Publication Number: WO 2020/055277 A1, Publication Date: March192020.
The World Intellectual Property Organization (WIPO)..
https://hdl.handle.net/21.15107/rcub_rimsi_2720

Branković Z, Ćirković J, Radojković A, Branković G, Jovanović J, Krnjajić S, Veljović-Jovanović S. BIOPOLYMER EMULSION FOR ACTIVE PACKAGING, USES AND METHOD OF MANUFACTURING. in International Patent Application No. PCT/RS2018/000013, International Filling Date: September 14, 2018., Applicants: University of Belgrade, Publication Number: WO 2020/055277 A1, Publication Date: March192020.. 2020;.
https://hdl.handle.net/21.15107/rcub_rimsi_2720 .

Branković, Zorica, Ćirković, Jovana, Radojković, Aleksandar, Branković, Goran, Jovanović, Jelena, Krnjajić, Slobodan, Veljović-Jovanović, Sonja, "BIOPOLYMER EMULSION FOR ACTIVE PACKAGING, USES AND METHOD OF MANUFACTURING" in International Patent Application No. PCT/RS2018/000013, International Filling Date: September 14, 2018., Applicants: University of Belgrade, Publication Number: WO 2020/055277 A1, Publication Date: March192020. (2020),
https://hdl.handle.net/21.15107/rcub_rimsi_2720 .

TY  - CHAP
AU  - Stankovic, Sladjan
AU  - Kostić, MIroslav
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2425
AB  - Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.
PB  - IntechOpen
T2  - In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
T1  - Practical Approaches to Pest Control: The Use of Natural Compounds
EP  - 18
SP  - 1
DO  - 10.5772/intechopen.91792
ER  - 

@inbook{
author = "Stankovic, Sladjan and Kostić, MIroslav and Kostić, Igor and Krnjajić, Slobodan",
year = "2020",
abstract = "Food production is challenged by different factors: climate changes, market competitiveness, food safety, public demands, environmental challenges, new and invasive pests, etc. Intensive food production must be protected against pests, which is nowadays impossible with traditional techniques. The use of eco-friendly biopesticides based on essential oils (EOs), plant extracts (PE), and inert dusts appears to be a complementary or alternative methodology to the conventional chemically synthesized insecticides. The use of such biopesticides reduces the adverse pesticide effects on human health and environment. Biopesticides can exhibit toxic, repellent, and antifeeding effects. Development of bio-insecticides tackles the problem of food safety and residues in fresh food. Innovation within this approach is the combination of several types of active ingredients with complementary effects. Essential oils are well-known compounds with insecticide or repellent activities. New approaches, tools, and products for ecological pest management may substantially decrease pesticide use, especially in fruit and vegetable production. A win-win strategy is to find an appropriate nature-based compound having impact on pests, together with pesticide use, when unavoidable. Toxic or repellent activity could be used for pest control in the field conditions, as well as attractiveness of some compounds for mass trapping, before pests cause significant economic damage.",
publisher = "IntechOpen",
journal = "In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen",
booktitle = "Practical Approaches to Pest Control: The Use of Natural Compounds",
pages = "18-1",
doi = "10.5772/intechopen.91792"
}

Stankovic, S., Kostić, M., Kostić, I.,& Krnjajić, S.. (2020). Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen
IntechOpen., 1-18.
https://doi.org/10.5772/intechopen.91792

Stankovic S, Kostić M, Kostić I, Krnjajić S. Practical Approaches to Pest Control: The Use of Natural Compounds. in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen. 2020;:1-18.
doi:10.5772/intechopen.91792 .

Stankovic, Sladjan, Kostić, MIroslav, Kostić, Igor, Krnjajić, Slobodan, "Practical Approaches to Pest Control: The Use of Natural Compounds" in In: Pests, Weeds and Diseases in Agricultural Crop and Animal Husbandry Production, IntechOpen (2020):1-18,
https://doi.org/10.5772/intechopen.91792 . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1974
AB  - The worldwide increased difficulty to contrast gastrointestinal nematode (GIN) infections in sheep, due to growing anthelmintic resistance, has led to find alternative helminth control strategies. This study aimed to evaluate the in vitro ovicidal activity of essential oils (EOs) of Juniperus communis and Achillea millefolium (two chemotypes) against GINs of sheep. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in Southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO and compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (Tween 80 3%, v/v). In both farms, coprocultures revealed the presence of four genera of GINs: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The inhibitory effect on GIN eggs’ hatchability varied from 81% to 96.75% for J. communis EO. When using A. millefolium EO, the ovicidal activity against GIN was 46.5-99.5% (type 1) and 69.6-97.25% (type 2). Furthermore, the effect of tested EOs was very high at concentrations of 50 mg/mL (96.75%), 12.5 mg/mL (95.5%) and 3.125 mg/mL (94.75%) of J. communis EO, whilst for A. millefolium EO at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) (type 1), and 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) (type 2), showing a similar effect (p>0.05) to the positive control (98%). Therefore, the findings of this study showed the anthelmintic potential of the tested EOs and highlight the importance of ethnopharmacology to control GIN infection in sheep.
PB  - COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230
C3  - 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”
T1  - Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep
EP  - 26
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1974
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "The worldwide increased difficulty to contrast gastrointestinal nematode (GIN) infections in sheep, due to growing anthelmintic resistance, has led to find alternative helminth control strategies. This study aimed to evaluate the in vitro ovicidal activity of essential oils (EOs) of Juniperus communis and Achillea millefolium (two chemotypes) against GINs of sheep. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in Southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO and compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (Tween 80 3%, v/v). In both farms, coprocultures revealed the presence of four genera of GINs: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The inhibitory effect on GIN eggs’ hatchability varied from 81% to 96.75% for J. communis EO. When using A. millefolium EO, the ovicidal activity against GIN was 46.5-99.5% (type 1) and 69.6-97.25% (type 2). Furthermore, the effect of tested EOs was very high at concentrations of 50 mg/mL (96.75%), 12.5 mg/mL (95.5%) and 3.125 mg/mL (94.75%) of J. communis EO, whilst for A. millefolium EO at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) (type 1), and 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) (type 2), showing a similar effect (p>0.05) to the positive control (98%). Therefore, the findings of this study showed the anthelmintic potential of the tested EOs and highlight the importance of ethnopharmacology to control GIN infection in sheep.",
publisher = "COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230",
journal = "4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”",
title = "Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep",
pages = "26-26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1974"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2020). Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep. in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”
COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230., 26-26.
https://hdl.handle.net/21.15107/rcub_rimsi_1974

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Maurelli MP, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep. in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”. 2020;:26-26.
https://hdl.handle.net/21.15107/rcub_rimsi_1974 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep" in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations” (2020):26-26,
https://hdl.handle.net/21.15107/rcub_rimsi_1974 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1885
AB  - The negative influence of gastrointestinal parasitism in sheep is growing today due to the development of anthelmintic resistance to commercial drugs. For this reason, researchers around the world are looking for new, alternative strategies for controlling these parasites. In this context, medicinal plants and their products are increasingly mentioned. The aim of this study was to evaluate in vitro ovicidal activity of common juniper (Juniperus communis L.) essential oil against sheep gastrointestinal nematodes. For that purpose, the egg hatch test was used, and the obtained results were analyzed by analysis of variance (ANOVA) followed by comparison with Tukey’s test (p<0.05). Faecal samples were collected from two independent, natural-infected farms located in Eboli (SA), Italy, whereby the coproculture examination identified the presence of four genera of sheep gastrointestinal nematodes: Haemonchus (53%), Trichostrngylus (29.5%), Teladorsagia (14.5%) and Chabertia (3%). Main components of common juniper essential oil identified by GC-MS analysis were α-pinene (40.46%), sabinene (14.04%), myrcene (8.87%) and limonene (4.95%). In vitro ovicidal activity was evaluated at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby J. communis essential oil showed high activity with the inhibitory effect on hatchability of 81-96.75%, depending on the concentration. For all concentration tested, the effect was statistically significantly higher compared to the negative control, while for the three highest concentrations the effect was similar to the positive control. The obtained results suggested that J. communis essential oil have high anthelmintic potential. However, these results need confirmation in further field examination.
AB  - Negativni uticaj gastrointestinalnih parazita kod ovaca u današnje vreme raste usled razvoja antihelmintičke rezistencije na komercijalne preparate. Iz tog razloga, istraživači širom sveta tragaju za novim, alternativnim strategijama u cilju kontrole ovih parazita. U tom kontekstu, lekovite biljke i njihovi proizvodi se sve češće spominju. Cilj ovog istraživanja je bio ispitati in vitro ovicidnu aktivnost esencijalnog ulja kleke (Juniperus communis L.) protiv gastrointestinalnih nematoda ovaca. Za ovu svrhu je upotrebljen egg hatch test, nakon čega su dobijeni rezultati obrađeni analizom varijanse i potom upoređeni Tukijevim testom (p<0.05). Uzorci fecesa su uzeti sa dve odvojene farme koje se nalaze u oblasti Eboli (SA), Italija, pri čemu je tokom ispitivanja koprokulture identifikovano prisustvo četiri roda gastrointestinalnih nematoda ovaca: Haemonchus (53%), Trichostrongylus (29.5%), Teladorsagia (14.5%) i Chaberita (3%). Najzastupljenije komponente etarskog ulja kleke identifikovane biohemijskim analizama su bile α-pinen (40.46%), sabinen (14.04%), mircen (8.87%) i limonen (4.95%*). In vitro ovicidna aktivnost je testirana za šest različitih koncentracija (50, 12.5, 3.125, 0.781, 0.195 i 0.049 mg/mL), pri čemu je etarsko ulje kleke pokazalo visoku aktivnost sa inhibitornim efektom na izleganje larvi od 81-96.75%, u zavisnosti od koncentracije. Kod svih ispitivanih koncentracija efekat je bio statistički značajno veći u poređenju sa negativnom kontrolom, dok je kod tri najveće koncentracije efekat bio sličan pozitivnoj kontroli. Dobijeni rezultati ukazuju na to da etarsko ulje kleke ima visok antihelmintički potencijal. Međutim, potrebno je ove rezultate potvrditi u budućim ispitivanjima u terenskim uslovima.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes
T1  - In vitro ovicidan efekat etarskog ulja kleke (Juniperus communis L.) na gastrointestinalne nematode ovaca
EP  - 159
IS  - 1
SP  - 152
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1885
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "The negative influence of gastrointestinal parasitism in sheep is growing today due to the development of anthelmintic resistance to commercial drugs. For this reason, researchers around the world are looking for new, alternative strategies for controlling these parasites. In this context, medicinal plants and their products are increasingly mentioned. The aim of this study was to evaluate in vitro ovicidal activity of common juniper (Juniperus communis L.) essential oil against sheep gastrointestinal nematodes. For that purpose, the egg hatch test was used, and the obtained results were analyzed by analysis of variance (ANOVA) followed by comparison with Tukey’s test (p<0.05). Faecal samples were collected from two independent, natural-infected farms located in Eboli (SA), Italy, whereby the coproculture examination identified the presence of four genera of sheep gastrointestinal nematodes: Haemonchus (53%), Trichostrngylus (29.5%), Teladorsagia (14.5%) and Chabertia (3%). Main components of common juniper essential oil identified by GC-MS analysis were α-pinene (40.46%), sabinene (14.04%), myrcene (8.87%) and limonene (4.95%). In vitro ovicidal activity was evaluated at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby J. communis essential oil showed high activity with the inhibitory effect on hatchability of 81-96.75%, depending on the concentration. For all concentration tested, the effect was statistically significantly higher compared to the negative control, while for the three highest concentrations the effect was similar to the positive control. The obtained results suggested that J. communis essential oil have high anthelmintic potential. However, these results need confirmation in further field examination., Negativni uticaj gastrointestinalnih parazita kod ovaca u današnje vreme raste usled razvoja antihelmintičke rezistencije na komercijalne preparate. Iz tog razloga, istraživači širom sveta tragaju za novim, alternativnim strategijama u cilju kontrole ovih parazita. U tom kontekstu, lekovite biljke i njihovi proizvodi se sve češće spominju. Cilj ovog istraživanja je bio ispitati in vitro ovicidnu aktivnost esencijalnog ulja kleke (Juniperus communis L.) protiv gastrointestinalnih nematoda ovaca. Za ovu svrhu je upotrebljen egg hatch test, nakon čega su dobijeni rezultati obrađeni analizom varijanse i potom upoređeni Tukijevim testom (p<0.05). Uzorci fecesa su uzeti sa dve odvojene farme koje se nalaze u oblasti Eboli (SA), Italija, pri čemu je tokom ispitivanja koprokulture identifikovano prisustvo četiri roda gastrointestinalnih nematoda ovaca: Haemonchus (53%), Trichostrongylus (29.5%), Teladorsagia (14.5%) i Chaberita (3%). Najzastupljenije komponente etarskog ulja kleke identifikovane biohemijskim analizama su bile α-pinen (40.46%), sabinen (14.04%), mircen (8.87%) i limonen (4.95%*). In vitro ovicidna aktivnost je testirana za šest različitih koncentracija (50, 12.5, 3.125, 0.781, 0.195 i 0.049 mg/mL), pri čemu je etarsko ulje kleke pokazalo visoku aktivnost sa inhibitornim efektom na izleganje larvi od 81-96.75%, u zavisnosti od koncentracije. Kod svih ispitivanih koncentracija efekat je bio statistički značajno veći u poređenju sa negativnom kontrolom, dok je kod tri najveće koncentracije efekat bio sličan pozitivnoj kontroli. Dobijeni rezultati ukazuju na to da etarsko ulje kleke ima visok antihelmintički potencijal. Međutim, potrebno je ove rezultate potvrditi u budućim ispitivanjima u terenskim uslovima.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes, In vitro ovicidan efekat etarskog ulja kleke (Juniperus communis L.) na gastrointestinalne nematode ovaca",
pages = "159-152",
number = "1",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1885"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2020). In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 1(1), 152-159.
https://hdl.handle.net/21.15107/rcub_rimsi_1885

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes. in Veterinarski Pregled. 2020;1(1):152-159.
https://hdl.handle.net/21.15107/rcub_rimsi_1885 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes" in Veterinarski Pregled, 1, no. 1 (2020):152-159,
https://hdl.handle.net/21.15107/rcub_rimsi_1885 .