TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1967
AB  - Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary
practice.
Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations
(50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo
Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated.
Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7%
depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT
with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%).
Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep
GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.
PB  - World Federation of Parasitologists
C3  - Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark
T1  - Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1967
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary
practice.
Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations
(50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo
Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated.
Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7%
depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT
with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%).
Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep
GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.",
publisher = "World Federation of Parasitologists",
journal = "Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark",
title = "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1967"
}

Štrbac, F., Bosco, A., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark
World Federation of Parasitologists..
https://hdl.handle.net/21.15107/rcub_rimsi_1967

Štrbac F, Bosco A, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Cringoli G, Rinaldi L. Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_1967 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study" in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1967 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1968
AB  - The development of anthelmintic resistance in gastrointestinal nematodes (GINs) requires the search for alternative strategies. The aim of this study was to examine in vitro and in vivo anthelmintic efficacy of essential oil (EO) of Satureja montana (L.) against GIN of sheep, as well as toxic effects in sheep, in order to evaluate the possibility of its use in the veterinary practice. In vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of EO. In addition, for the in vivo Faecal Egg Count Reduction Test (FECRT), S. montana EO was administered orally to sheep of two different farms
in southern Italy at the mean dose of 150 mg/kg. The chemical composition of EO was determined by GC-MS. In the EHT, S. montana EO showed ovicidal potential against sheep GINs with inhibition of egg hatchability that varied from 17.3-83.0% depending on the used concentration. The EO also showed in vivo anthelmintic potential with the total reduction of FEC of 15.7% and 33.0% at days 7 and 14 after treatment, respectively. No toxic effects were observed during clinical evaluation, blood count and liver function tests of sheep. The main represented compounds of EO were p-cymene (42.8%), carvacrol (28.1%) and γ-terpinene (14.6%). The obtained results suggest that S. montana EO may be used in the sustainable future management of GIN infections in sheep as a complementary and sustainable method to reduce the use of chemicals and to counteract anthelmintic resistance.
PB  - COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230
C3  - Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece
T1  - In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1968
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The development of anthelmintic resistance in gastrointestinal nematodes (GINs) requires the search for alternative strategies. The aim of this study was to examine in vitro and in vivo anthelmintic efficacy of essential oil (EO) of Satureja montana (L.) against GIN of sheep, as well as toxic effects in sheep, in order to evaluate the possibility of its use in the veterinary practice. In vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of EO. In addition, for the in vivo Faecal Egg Count Reduction Test (FECRT), S. montana EO was administered orally to sheep of two different farms
in southern Italy at the mean dose of 150 mg/kg. The chemical composition of EO was determined by GC-MS. In the EHT, S. montana EO showed ovicidal potential against sheep GINs with inhibition of egg hatchability that varied from 17.3-83.0% depending on the used concentration. The EO also showed in vivo anthelmintic potential with the total reduction of FEC of 15.7% and 33.0% at days 7 and 14 after treatment, respectively. No toxic effects were observed during clinical evaluation, blood count and liver function tests of sheep. The main represented compounds of EO were p-cymene (42.8%), carvacrol (28.1%) and γ-terpinene (14.6%). The obtained results suggest that S. montana EO may be used in the sustainable future management of GIN infections in sheep as a complementary and sustainable method to reduce the use of chemicals and to counteract anthelmintic resistance.",
publisher = "COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230",
journal = "Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece",
title = "In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1968"
}

Štrbac, F., Bosco, A., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep. in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece
COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230..
https://hdl.handle.net/21.15107/rcub_rimsi_1968

Štrbac F, Bosco A, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Cringoli G, Rinaldi L. In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep. in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_1968 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep" in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1968 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1970
AB  - The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
C3  - Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
T1  - In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1970
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan",
title = "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1970"
}

Štrbac, F., Bosco, A., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
Unique Scientific Publishers, Faisalabad, Pakistan., 27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970

Štrbac F, Bosco A, Stojanović D, Ratajac R, Simin N, Orčić D, Petrović K, Cringoli G, Rinaldi L. In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes" in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan (2022):27-27,
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Petrović, Kosta
AU  - Ratajac, Radomir
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1976
AB  - The development and spread of anthelmintic resistance to commercial drugs represent a major problem in veterinary medicine, which justifies the search for alternatives. Within that context, sage (Salvia officinalis L.) is a well-known medicinal plant that has been cultivated for centuries due to its healing properties. The aim of this study was to determine the chemical composition of the sage essential oil and to evaluate its anthelmintic properties against sheep gastrointestinal nematodes. Chemical characterization was done by GC-MS analyses, and the anthelmintic potential of tested
samples was evaluated by in vitro egg hatch test in a concentration range of 0.0125-50 mg/mL. The most represented identified compounds were α-thujone (38.76%), camphor (19.75%) and 1,8-cineole (8.40%), camphene (5.36%) and α-humulene (4.15%) out of a total of 27 identified compounds. The ovicidal activity of sage essential oil, reflected in the inhibition of egg hatchability of the eggs of sheep gastrointestinal nematodes, varied from 15.0-89.0% depending on the used concentration, with a calculated IC50 value of 0.53 mg/mL. The obtained results indicate that the compounds from sage essential oil posess high anthelmintic properties and should be tested in further in vivo studies. Sage essential oil could find application in veterinary medicine in the context of the development of new natural anthelmintic agents for the control of sheep gastrointestinal nematodes, which could have high significance from economic point of view.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
T1  - Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1976
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Bosco, Antonio and Stojanović, Dragica and Petrović, Kosta and Ratajac, Radomir and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The development and spread of anthelmintic resistance to commercial drugs represent a major problem in veterinary medicine, which justifies the search for alternatives. Within that context, sage (Salvia officinalis L.) is a well-known medicinal plant that has been cultivated for centuries due to its healing properties. The aim of this study was to determine the chemical composition of the sage essential oil and to evaluate its anthelmintic properties against sheep gastrointestinal nematodes. Chemical characterization was done by GC-MS analyses, and the anthelmintic potential of tested
samples was evaluated by in vitro egg hatch test in a concentration range of 0.0125-50 mg/mL. The most represented identified compounds were α-thujone (38.76%), camphor (19.75%) and 1,8-cineole (8.40%), camphene (5.36%) and α-humulene (4.15%) out of a total of 27 identified compounds. The ovicidal activity of sage essential oil, reflected in the inhibition of egg hatchability of the eggs of sheep gastrointestinal nematodes, varied from 15.0-89.0% depending on the used concentration, with a calculated IC50 value of 0.53 mg/mL. The obtained results indicate that the compounds from sage essential oil posess high anthelmintic properties and should be tested in further in vivo studies. Sage essential oil could find application in veterinary medicine in the context of the development of new natural anthelmintic agents for the control of sheep gastrointestinal nematodes, which could have high significance from economic point of view.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"",
title = "Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1976"
}

Štrbac, F., Simin, N., Orčić, D., Bosco, A., Stojanović, D., Petrović, K., Ratajac, R., Cringoli, G.,& Rinaldi, L.. (2022). Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 143-143.
https://hdl.handle.net/21.15107/rcub_rimsi_1976

Štrbac F, Simin N, Orčić D, Bosco A, Stojanović D, Petrović K, Ratajac R, Cringoli G, Rinaldi L. Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry". 2022;:143-143.
https://hdl.handle.net/21.15107/rcub_rimsi_1976 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Bosco, Antonio, Stojanović, Dragica, Petrović, Kosta, Ratajac, Radomir, Cringoli, Giuseppe, Rinaldi, Laura, "Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes" in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry" (2022):143-143,
https://hdl.handle.net/21.15107/rcub_rimsi_1976 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1924
AB  - Upotreba biljnih preparata kao što su etarska ulja, za različite indikacije u veterinarskoj medicini predstavlja veoma aktuelno i perspektivno polje. U tom kontekstu, najčešće se ispituju i dokazuju antibakterijsko, antifungalno i antiparazitsko, ali i mnoga druga lekovita dejstva ovih preparata. Zbog toga se etarska ulja sve više koriste za različite namene, kao što su prevencija dijareje prasadi ili tretiranje kokcidioze živine. Međutim, usled njihove nestabilne prirode i sklonosti ka isparavanju može doći do degradacije i delimične inaktivacije njihovih aktivnih sastojaka u organizmu, što dovodi do ograničene efikasnosti etarskih ulja nakon primene in vivo. Potencijalno rešenje za ovaj problem, mnogi istraživači vide u inovativnim tehnikama inkapsulacije. One predstavljaju postupak zaštite aktivnih komponenti, fizičkim ili hemijskim procesima, pri čemu se formira zaštitni omotač. Zbog toga su ove tehnike emulzifikacije i nanotehnologije našle primenu u različitim granama (industrija hrane, farmacija), a mogle bi biti veoma korisne i kada je u pitanju primena etarskih ulja. Naime, inkapsulacijom se smanjuje interakcija aktivnih supstanci sa različitim faktorima kao i brzina isparavanja, čime bi se mogla umanjiti inaktivacija sastojaka etarskih ulja, povećati bioraspoloživost, a samim tim i efikasnost. Ovo je posebno značajno za primenu protiv bakterija i endoparazita u gastrointestinalnom traktu. Primenom ovih tehnika se omogućava i kontrolisano oslobađanje aktivnih supstanci, što takođe može biti značajno prilikom pojedinih vidova primene kao što je antiektoparazitska. Na kraju, inkapsulacijom se povećava jednostavnost rukovanja aktivnim supstancama, ali i umanjuje neprijatan miris i ukus, što je veoma bitno prilikom primene etarskih ulja u hrani za životinje. Iz svega navedenog se može zaključiti da tehnike inkapsulacije imaju višestruki značaj kada je u pitanju primena etarskih ulja u veterinarskoj praksi, omogućavajući jednostavniju, praktičniju i efikasniju a samim tim i širu primenu ovih prirodnih proizvoda.
AB  - The use of herbal preparations such as essential oils for various indications in veterinary medicine presents a modern and promising field. Within that context, antibacterial, antifungal and antiparasitic, as well as many other medicinal effects of these substances are most often examined. For that reason, essential oils are increasingly used for different purposes, such as prevention of diarrhoea in piglets or coccidiosis treatment in poultry. However, their unstable and volatile nature may lead to degradation and partial deactivation of their active ingredients in the organism after some in vivo applications. As a potential solution for this problem, many researchers consider innovative encapsulation techniques. They represent the process of protecting the active components by physical or chemical processes, whereby a protective coating is formed. Therefore, these techniques such as emulsification or nanotechnology have found application in various branches (food industry, pharmacy) and could be very useful for the application of essential oils. Namely, encapsulation reduces the interaction of active substances with various factors as well as the rate of evaporation, which could reduce the deactivation of essential oil ingredients, increase bioavailability and thus efficiency (significant for use against the bacteria and endoparasites in the intestinal tract). The application of these techniques also enables the controlled release of active substances, which can also be important for some applications such as antiectoparasitic. Finally, encapsulation increases the ease of application and also reduces unpleasant smells and tastes, which is very important for the use of essential oils in animal feed. From all the above, it can be concluded that encapsulation techniques have multiple implications for the application of essential oils in veterinary practice with enabling simpler, more practical, efficient, and thus wider application of these natural products.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)
C3  - Zbornik radova Zdravstvena zaštita i reprodukcija životinja
T1  - Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini
T1  - Encapsulation and its Significance for Application of Essential Oils in Veterinary Medicine
EP  - 46
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1924
ER  - 

@conference{
author = "Štrbac, Filip and Stojanović, Dragica and Ratajac, Radomir and Petrović, Kosta",
year = "2022",
abstract = "Upotreba biljnih preparata kao što su etarska ulja, za različite indikacije u veterinarskoj medicini predstavlja veoma aktuelno i perspektivno polje. U tom kontekstu, najčešće se ispituju i dokazuju antibakterijsko, antifungalno i antiparazitsko, ali i mnoga druga lekovita dejstva ovih preparata. Zbog toga se etarska ulja sve više koriste za različite namene, kao što su prevencija dijareje prasadi ili tretiranje kokcidioze živine. Međutim, usled njihove nestabilne prirode i sklonosti ka isparavanju može doći do degradacije i delimične inaktivacije njihovih aktivnih sastojaka u organizmu, što dovodi do ograničene efikasnosti etarskih ulja nakon primene in vivo. Potencijalno rešenje za ovaj problem, mnogi istraživači vide u inovativnim tehnikama inkapsulacije. One predstavljaju postupak zaštite aktivnih komponenti, fizičkim ili hemijskim procesima, pri čemu se formira zaštitni omotač. Zbog toga su ove tehnike emulzifikacije i nanotehnologije našle primenu u različitim granama (industrija hrane, farmacija), a mogle bi biti veoma korisne i kada je u pitanju primena etarskih ulja. Naime, inkapsulacijom se smanjuje interakcija aktivnih supstanci sa različitim faktorima kao i brzina isparavanja, čime bi se mogla umanjiti inaktivacija sastojaka etarskih ulja, povećati bioraspoloživost, a samim tim i efikasnost. Ovo je posebno značajno za primenu protiv bakterija i endoparazita u gastrointestinalnom traktu. Primenom ovih tehnika se omogućava i kontrolisano oslobađanje aktivnih supstanci, što takođe može biti značajno prilikom pojedinih vidova primene kao što je antiektoparazitska. Na kraju, inkapsulacijom se povećava jednostavnost rukovanja aktivnim supstancama, ali i umanjuje neprijatan miris i ukus, što je veoma bitno prilikom primene etarskih ulja u hrani za životinje. Iz svega navedenog se može zaključiti da tehnike inkapsulacije imaju višestruki značaj kada je u pitanju primena etarskih ulja u veterinarskoj praksi, omogućavajući jednostavniju, praktičniju i efikasniju a samim tim i širu primenu ovih prirodnih proizvoda., The use of herbal preparations such as essential oils for various indications in veterinary medicine presents a modern and promising field. Within that context, antibacterial, antifungal and antiparasitic, as well as many other medicinal effects of these substances are most often examined. For that reason, essential oils are increasingly used for different purposes, such as prevention of diarrhoea in piglets or coccidiosis treatment in poultry. However, their unstable and volatile nature may lead to degradation and partial deactivation of their active ingredients in the organism after some in vivo applications. As a potential solution for this problem, many researchers consider innovative encapsulation techniques. They represent the process of protecting the active components by physical or chemical processes, whereby a protective coating is formed. Therefore, these techniques such as emulsification or nanotechnology have found application in various branches (food industry, pharmacy) and could be very useful for the application of essential oils. Namely, encapsulation reduces the interaction of active substances with various factors as well as the rate of evaporation, which could reduce the deactivation of essential oil ingredients, increase bioavailability and thus efficiency (significant for use against the bacteria and endoparasites in the intestinal tract). The application of these techniques also enables the controlled release of active substances, which can also be important for some applications such as antiectoparasitic. Finally, encapsulation increases the ease of application and also reduces unpleasant smells and tastes, which is very important for the use of essential oils in animal feed. From all the above, it can be concluded that encapsulation techniques have multiple implications for the application of essential oils in veterinary practice with enabling simpler, more practical, efficient, and thus wider application of these natural products.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)",
journal = "Zbornik radova Zdravstvena zaštita i reprodukcija životinja",
title = "Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini, Encapsulation and its Significance for Application of Essential Oils in Veterinary Medicine",
pages = "46-37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1924"
}

Štrbac, F., Stojanović, D., Ratajac, R.,& Petrović, K.. (2022). Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja
Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)., 37-46.
https://hdl.handle.net/21.15107/rcub_rimsi_1924

Štrbac F, Stojanović D, Ratajac R, Petrović K. Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja. 2022;:37-46.
https://hdl.handle.net/21.15107/rcub_rimsi_1924 .

Štrbac, Filip, Stojanović, Dragica, Ratajac, Radomir, Petrović, Kosta, "Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini" in Zbornik radova Zdravstvena zaštita i reprodukcija životinja (2022):37-46,
https://hdl.handle.net/21.15107/rcub_rimsi_1924 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1908
AB  - Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio odrediti hemijski sastav etarskog ulja korijandera (Coriander sativum L.) i ispitati njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden pri osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka od čega je 15 identifikovano, iz različitih hemijskih grupa. Dominantan sastojak je bio linalool (84,53%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti. U svakom slučaju, i ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije.
AB  - The development of resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to huge monetary losses in many parts of the world. Therefore, new sustainable strategies for the control of these parasites are being actively sought, among which are included various alternative options such as plant essential oils. The aim of this study was to determine the chemical composition of the coriander (Coriander sativum L.) essential oil and to evaluate its anthelmintic potential against sheep gastrointestinal nematodes. Qualitative and semi-quantitative chemical characterization of the essential oils was performed by gas chromatography-mass spectrometry (GC-MS) analyses. The anthelmintic potential of the essential oil was evaluated using the in vitro egg hatch test (EHT), which was performed at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole at concentrations of 0.025 and 0.0125 mg/mL was used as a positive control, and emulsifier 3% Tween 80 v/v and distilled water as negative controls. GC-MS analysis revealed the rich chemical composition of the tested oil, where a total of 17 different ingredients were found of which 15 were identified, from different chemical groups. The dominant ingredient was linalool (84.53%) with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). EHT results showed a high anthelmintic potential of the coriander oil with an inhibition of egg hatchability i.e. ovicidal effect of 29.0-88.7% depending on the used concentration, with the obtained IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). The obtained results indicate the possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes in sheep, after further in vivo studies and toxicity studies. In any case, this study is another confirmation of the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and in combating anthelmintic resistance.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav
T1  - Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) and its Chemical Composition
EP  - 341
SP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1908
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio odrediti hemijski sastav etarskog ulja korijandera (Coriander sativum L.) i ispitati njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden pri osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka od čega je 15 identifikovano, iz različitih hemijskih grupa. Dominantan sastojak je bio linalool (84,53%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti. U svakom slučaju, i ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije., The development of resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to huge monetary losses in many parts of the world. Therefore, new sustainable strategies for the control of these parasites are being actively sought, among which are included various alternative options such as plant essential oils. The aim of this study was to determine the chemical composition of the coriander (Coriander sativum L.) essential oil and to evaluate its anthelmintic potential against sheep gastrointestinal nematodes. Qualitative and semi-quantitative chemical characterization of the essential oils was performed by gas chromatography-mass spectrometry (GC-MS) analyses. The anthelmintic potential of the essential oil was evaluated using the in vitro egg hatch test (EHT), which was performed at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole at concentrations of 0.025 and 0.0125 mg/mL was used as a positive control, and emulsifier 3% Tween 80 v/v and distilled water as negative controls. GC-MS analysis revealed the rich chemical composition of the tested oil, where a total of 17 different ingredients were found of which 15 were identified, from different chemical groups. The dominant ingredient was linalool (84.53%) with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). EHT results showed a high anthelmintic potential of the coriander oil with an inhibition of egg hatchability i.e. ovicidal effect of 29.0-88.7% depending on the used concentration, with the obtained IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). The obtained results indicate the possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes in sheep, after further in vivo studies and toxicity studies. In any case, this study is another confirmation of the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and in combating anthelmintic resistance.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav, Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) and its Chemical Composition",
pages = "341-338",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1908"
}

Štrbac, F., Bosco, A., Petrović, K., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Cringoli, G.,& Rinaldi, L.. (2022). Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav. in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 338-341.
https://hdl.handle.net/21.15107/rcub_rimsi_1908

Štrbac F, Bosco A, Petrović K, Stojanović D, Ratajac R, Simin N, Orčić D, Cringoli G, Rinaldi L. Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav. in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2022;:338-341.
https://hdl.handle.net/21.15107/rcub_rimsi_1908 .

Štrbac, Filip, Bosco, Antonio, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Cringoli, Giuseppe, Rinaldi, Laura, "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav" in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2022):338-341,
https://hdl.handle.net/21.15107/rcub_rimsi_1908 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1894
AB  - Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio da se odredi hemijski sastav etarskog ulja korijandera (Coriandrum sativum L.) i da se ispita njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden sa osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka iz različitih hemijskih grupa, od čega je 15 identifikovano. Dominantan sastojak je bio linalool (84,5%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije. Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti.
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to enormous economic losses in many parts of the world. For this reason, new sustainable strategies for the control of these parasites are being actively searched, which include the use of different alternatives such as plant essential oils. The aim of this study was to determine the chemical composition of the essential oil of coriander (Coriandrum sativum L.) and to test its anthelmintic potential against gastrointestinal nematodes of sheep. Qualitative and semi-quantitative chemical characterization of the essential oil was performed using gas chromatography coupled with mass spectrometric detection (GC-MS analysis). The anthelmintic potential of the essential oil was tested using the in vitro egg hatch test (EHT), which was carried out at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole in concentrations of 0.025 and 0.0125 mg/mL was used as the positive control, and the emulsifier 3% Tween 80 v/v and distilled water was used as the negative control. GC-MS analyzes revealed a rich chemical composition of the tested oil, where a total of 17 different ingredients from different chemical groups were found, of which 15 were identified. The dominant ingredient was linalool (84.5%) along with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). The EHT results showed a high anthelmintic potential of coriander oil with inhibition of egg hatching, i.e. an ovicidal effect of 29.0-88.7% depending on the concentration, with an IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). This study confirmed the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and the combatting anthelmintic resistance. The obtained results indicate a possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes of sheep, after further in vivo tests and toxicity studies.
PB  - JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka
T2  - Veterinarski Žurnal Republike Srpske
T1  - Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca
T1  - Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) in Sheep
EP  - 126
IS  - 1-2
SP  - 101
VL  - XXII
DO  - 10.7251/VETJSR2201101S
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio da se odredi hemijski sastav etarskog ulja korijandera (Coriandrum sativum L.) i da se ispita njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden sa osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka iz različitih hemijskih grupa, od čega je 15 identifikovano. Dominantan sastojak je bio linalool (84,5%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije. Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti., The development of anthelmintic resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to enormous economic losses in many parts of the world. For this reason, new sustainable strategies for the control of these parasites are being actively searched, which include the use of different alternatives such as plant essential oils. The aim of this study was to determine the chemical composition of the essential oil of coriander (Coriandrum sativum L.) and to test its anthelmintic potential against gastrointestinal nematodes of sheep. Qualitative and semi-quantitative chemical characterization of the essential oil was performed using gas chromatography coupled with mass spectrometric detection (GC-MS analysis). The anthelmintic potential of the essential oil was tested using the in vitro egg hatch test (EHT), which was carried out at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole in concentrations of 0.025 and 0.0125 mg/mL was used as the positive control, and the emulsifier 3% Tween 80 v/v and distilled water was used as the negative control. GC-MS analyzes revealed a rich chemical composition of the tested oil, where a total of 17 different ingredients from different chemical groups were found, of which 15 were identified. The dominant ingredient was linalool (84.5%) along with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). The EHT results showed a high anthelmintic potential of coriander oil with inhibition of egg hatching, i.e. an ovicidal effect of 29.0-88.7% depending on the concentration, with an IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). This study confirmed the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and the combatting anthelmintic resistance. The obtained results indicate a possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes of sheep, after further in vivo tests and toxicity studies.",
publisher = "JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka",
journal = "Veterinarski Žurnal Republike Srpske",
title = "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca, Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) in Sheep",
pages = "126-101",
number = "1-2",
volume = "XXII",
doi = "10.7251/VETJSR2201101S"
}

Štrbac, F., Bosco, A., Petrović, K., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Cringoli, G.,& Rinaldi, L.. (2022). Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca. in Veterinarski Žurnal Republike Srpske
JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka., XXII(1-2), 101-126.
https://doi.org/10.7251/VETJSR2201101S

Štrbac F, Bosco A, Petrović K, Stojanović D, Ratajac R, Simin N, Orčić D, Cringoli G, Rinaldi L. Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca. in Veterinarski Žurnal Republike Srpske. 2022;XXII(1-2):101-126.
doi:10.7251/VETJSR2201101S .

Štrbac, Filip, Bosco, Antonio, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Cringoli, Giuseppe, Rinaldi, Laura, "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca" in Veterinarski Žurnal Republike Srpske, XXII, no. 1-2 (2022):101-126,
https://doi.org/10.7251/VETJSR2201101S . .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Knežević, Goran
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Bosco, Antonio
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1884
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.
PB  - MDPI
T2  - Animals
T1  - A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1
SP  - 45
VL  - 13
DO  - 10.3390/ani13010045
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Knežević, Goran and Cringoli, Giuseppe and Rinaldi, Laura and Bosco, Antonio",
year = "2022",
abstract = "The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.",
publisher = "MDPI",
journal = "Animals",
title = "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1",
pages = "45",
volume = "13",
doi = "10.3390/ani13010045"
}

Štrbac, F., Krnjajić, S., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Knežević, G., Cringoli, G., Rinaldi, L.,& Bosco, A.. (2022). A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals
MDPI., 13(1), 45.
https://doi.org/10.3390/ani13010045

Štrbac F, Krnjajić S, Maurelli MP, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Knežević G, Cringoli G, Rinaldi L, Bosco A. A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals. 2022;13(1):45.
doi:10.3390/ani13010045 .

Štrbac, Filip, Krnjajić, Slobodan, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Knežević, Goran, Cringoli, Giuseppe, Rinaldi, Laura, Bosco, Antonio, "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Animals, 13, no. 1 (2022):45,
https://doi.org/10.3390/ani13010045 . .

TY  - JOUR
AU  - Petrović, Kosta
AU  - Djokovic, Radojica
AU  - Cincović, Marko
AU  - Hristovska, Talija
AU  - Lalović, Miroslav
AU  - Petrović, Miloš
AU  - Majkić, Mira
AU  - Došenović Marinković, Maja
AU  - Anđušić, Ljiljana
AU  - Devečerski, Gordana
AU  - Stojanović, Dragica
AU  - Štrbac, Filip
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1883
AB  - Previous experimental models on cows have examined the difference in the metabolic adaptation in cows after niacin administration, without identifying the most important mediators between niacin administration and its biological effects, namely active forms of niacin. All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The aim of this study was to determine the influence of niacin administration in periparturient period on NAD, NADP and the NAD:NADP ratio and to determine relationship between these indicators of an active form of niacin with metabolic parameters in cow blood. The study included 90 healthy cows: 45 cows receiving niacin and 45 cows were negative control. The niacin group was treated with nicotinic acid for two weeks before, as well as two weeks after parturition. Nicotinic acid was applied per os with feed. In cows receiving niacin, there was a significantly higher concentration of NAD and NADP, but the NAD:NADP ratio did not differ compared with control. All three indicators were able to separate cows who received and who did not receive additional niacin. NAD and NADP are good indicators of the availability of niacin from additional sources. The NAD:NADP ratio is a good indicator of the biological effect of applied niacin on metabolites in cows due to its correlation with a number of metabolites: positive correlation with glucose, insulin, glucose to insulin ratio and the revised quantitative insulin sensitivity check index (RQUICKIBHB) of insulin resistance, triglycerides and cholesterol, and a negative correlation with nonesterified fatty acid (NEFA), beta hydroxybutyrate (BHB), gamma-glutamyltranspherase (GGT) and urea in cows receiving niacin. The same amount of added niacin in feed can produce different concentrations of NAD, NADP and NAD:NADP in the blood, and this was not related to their concentration before the addition of niacin. The change in the concentration of the active form of niacin (NAD, NADP and NAD:NADP) further correlates with the concentration of metabolic parameters, which indicates that the intensity of the biological effect of additional niacin can be accurately determined only if we know the concentrations of its active forms in blood. Under basal conditions (without additional niacin), active forms of niacin that already exist in the blood do not show significant correlations with metabolic parameters.
PB  - MDPI
T2  - Animals
T1  - Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation
IS  - 12
SP  - 1524
VL  - 12
DO  - 10.3390/ani12121524
ER  - 

@article{
author = "Petrović, Kosta and Djokovic, Radojica and Cincović, Marko and Hristovska, Talija and Lalović, Miroslav and Petrović, Miloš and Majkić, Mira and Došenović Marinković, Maja and Anđušić, Ljiljana and Devečerski, Gordana and Stojanović, Dragica and Štrbac, Filip",
year = "2022",
abstract = "Previous experimental models on cows have examined the difference in the metabolic adaptation in cows after niacin administration, without identifying the most important mediators between niacin administration and its biological effects, namely active forms of niacin. All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The aim of this study was to determine the influence of niacin administration in periparturient period on NAD, NADP and the NAD:NADP ratio and to determine relationship between these indicators of an active form of niacin with metabolic parameters in cow blood. The study included 90 healthy cows: 45 cows receiving niacin and 45 cows were negative control. The niacin group was treated with nicotinic acid for two weeks before, as well as two weeks after parturition. Nicotinic acid was applied per os with feed. In cows receiving niacin, there was a significantly higher concentration of NAD and NADP, but the NAD:NADP ratio did not differ compared with control. All three indicators were able to separate cows who received and who did not receive additional niacin. NAD and NADP are good indicators of the availability of niacin from additional sources. The NAD:NADP ratio is a good indicator of the biological effect of applied niacin on metabolites in cows due to its correlation with a number of metabolites: positive correlation with glucose, insulin, glucose to insulin ratio and the revised quantitative insulin sensitivity check index (RQUICKIBHB) of insulin resistance, triglycerides and cholesterol, and a negative correlation with nonesterified fatty acid (NEFA), beta hydroxybutyrate (BHB), gamma-glutamyltranspherase (GGT) and urea in cows receiving niacin. The same amount of added niacin in feed can produce different concentrations of NAD, NADP and NAD:NADP in the blood, and this was not related to their concentration before the addition of niacin. The change in the concentration of the active form of niacin (NAD, NADP and NAD:NADP) further correlates with the concentration of metabolic parameters, which indicates that the intensity of the biological effect of additional niacin can be accurately determined only if we know the concentrations of its active forms in blood. Under basal conditions (without additional niacin), active forms of niacin that already exist in the blood do not show significant correlations with metabolic parameters.",
publisher = "MDPI",
journal = "Animals",
title = "Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation",
number = "12",
pages = "1524",
volume = "12",
doi = "10.3390/ani12121524"
}

Petrović, K., Djokovic, R., Cincović, M., Hristovska, T., Lalović, M., Petrović, M., Majkić, M., Došenović Marinković, M., Anđušić, L., Devečerski, G., Stojanović, D.,& Štrbac, F.. (2022). Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation. in Animals
MDPI., 12(12), 1524.
https://doi.org/10.3390/ani12121524

Petrović K, Djokovic R, Cincović M, Hristovska T, Lalović M, Petrović M, Majkić M, Došenović Marinković M, Anđušić L, Devečerski G, Stojanović D, Štrbac F. Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation. in Animals. 2022;12(12):1524.
doi:10.3390/ani12121524 .

Petrović, Kosta, Djokovic, Radojica, Cincović, Marko, Hristovska, Talija, Lalović, Miroslav, Petrović, Miloš, Majkić, Mira, Došenović Marinković, Maja, Anđušić, Ljiljana, Devečerski, Gordana, Stojanović, Dragica, Štrbac, Filip, "Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation" in Animals, 12, no. 12 (2022):1524,
https://doi.org/10.3390/ani12121524 . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1909
AB  - Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Због тога се ови биљни производи од давнина користе у народној медицини многих култура широм света, а могли би наћи широку примену и у ветеринарској медицини. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразатских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине које их чине погодним за употребу, као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације већ користе, као што су шампони на природној бази који најчешће садрже више различитих етарских уља или њихових састојака у оптималној сразмери. Међутим, главно ограничење за употребу ових биљних производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највеероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП- глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова ефикасност као и омогућити контролисано ослобађање, што све заједно може допринети смањењу потребне дозе. На тај начин се може додатно повећати сигурност њихове примене.
AB  - Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. These herbal products have long utilized in folk medicine in many cultures around the world, and also could be widely used in veterinary medicine. However, little is known about their use in animals including dogs and cats, although researches so far highlighted different possible application. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties that make them suitable for use, such as rich chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain formulations are already in use, such as natural shampoos that usually contain several different essential oils or their ingredients in optimal proportions. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that participate in metabolic processes (eg UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the way of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their efficacy as well as enable controlled release, which all may contribute to reducing the required dose. In this way, the safety of their application can be further increased.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Могућност и ограничења примене етарских уља код паса и мачака
T1  - Possibility and Limitations of the Use of Essential Oils in Dogs and Cats
EP  - 202
SP  - 201
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1909
ER  - 

@conference{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir",
year = "2021",
abstract = "Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Због тога се ови биљни производи од давнина користе у народној медицини многих култура широм света, а могли би наћи широку примену и у ветеринарској медицини. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразатских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине које их чине погодним за употребу, као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације већ користе, као што су шампони на природној бази који најчешће садрже више различитих етарских уља или њихових састојака у оптималној сразмери. Међутим, главно ограничење за употребу ових биљних производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највеероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП- глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова ефикасност као и омогућити контролисано ослобађање, што све заједно може допринети смањењу потребне дозе. На тај начин се може додатно повећати сигурност њихове примене., Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. These herbal products have long utilized in folk medicine in many cultures around the world, and also could be widely used in veterinary medicine. However, little is known about their use in animals including dogs and cats, although researches so far highlighted different possible application. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties that make them suitable for use, such as rich chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain formulations are already in use, such as natural shampoos that usually contain several different essential oils or their ingredients in optimal proportions. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that participate in metabolic processes (eg UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the way of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their efficacy as well as enable controlled release, which all may contribute to reducing the required dose. In this way, the safety of their application can be further increased.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Могућност и ограничења примене етарских уља код паса и мачака, Possibility and Limitations of the Use of Essential Oils in Dogs and Cats",
pages = "202-201",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1909"
}

Štrbac, F., Petrović, K., Stojanović, D.,& Ratajac, R.. (2021). Могућност и ограничења примене етарских уља код паса и мачака. in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 201-202.
https://hdl.handle.net/21.15107/rcub_rimsi_1909

Štrbac F, Petrović K, Stojanović D, Ratajac R. Могућност и ограничења примене етарских уља код паса и мачака. in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2021;:201-202.
https://hdl.handle.net/21.15107/rcub_rimsi_1909 .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, "Могућност и ограничења примене етарских уља код паса и мачака" in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2021):201-202,
https://hdl.handle.net/21.15107/rcub_rimsi_1909 .

TY  - JOUR
AU  - Stojanović, Dragica
AU  - Cincović, Marko
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - CRISTINA, ROMEO TEODOR
AU  - Kovačević, Zorana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1880
AB  - Sepsis can be defined as a multisystem response of the organism on bacterial infection agents. The aim of this study is to provides an extensive analysis of associations between numerous routine biochemical and hematological markers in function of type of sepsis caused by experimental Gram-positive (G+, Staphylococcus aureus), Gram-negative (G-, Escherichia coli) and mixed (MIX) microorganism in laboratory rats model. In G- and G+ sepsis higher values of erythrocytes, total protein, albumin, creatinine, sodium and plasma osmolarity were noted and lower triglycerides values compared to MIX sepsis. Existence of statistically significant interaction between time and type of sepsis was showed that values of erythrocytes, neutrophiles, LUC, glucose, total protein, urea, Na, Cl, osmolarity and LDH were changed during time in function of sepsis type. Distinguish of animals with G+, G- or MIX sepsis is not possible because principal component analysis (PCA) demonstrated similar expression of blood hematological and metabolic parameters in animals regardless type of experimental sepsis (G+, G- or MIX), with minimal deviation of animal with MIX sepsis from animals with sepsis caused by pure culture (G+ or G-). Correlation coefficient analysis showed 507 significant correlations between routine blood parameters (207 in G-, 186 in G+ and 114 in MIX) and some of them are in function of type of sepsis. Distinguish of type of sepsis in function of blood parameters is not possible. After extraction of two components they load by blood parameters was similar in all type of sepsis (positive load with different type of white blood cells, PLT, glucose, amylase, Cl, urea and osmolarity, and negative load with albumin, total protein, cholesterol, creatinine and ALT). Although our results demonstrated difference in values and correlation coefficients for routine blood biochemistry and hematology parameters, multiparametric statistic showed they not be a useful tool for distinguishing type of sepsis according bacterial causative.
AB  - Sepsa se može definisati kao multisistemski odgovor organizma na uzročnike bakterijske infekcije. Cilj ove studije je da pruži opsežnu analizu povezanosti brojnih rutinskih biohemijskih i hematoloških markera u funkciji tipa sepse izazvane eksperimentalnim Gram-pozitivnim (G+, Staphylococcus aureus), Gramnegativnim (G-, Escherichia coli) i mešani (MIX) mikroorganizam u modelu laboratorijskih pacova. U G- i G+ sepsi su zabeležene više vrednosti eritrocita, ukupnog proteina, albumina, kreatinina, natrijuma i osmolarnosti plazme i niže vrednosti triglicerida u odnosu na MIX sepsu. Utvrđeno je postojanje statistički značajne interakcije između vremena i tipa sepse da su se vrednosti eritrocita, neutrofila, LUC, glukoze, ukupnog proteina, uree, Na, Cl, osmolarnosti i LDH menjale tokom vremena u funkciji tipa sepse. Razlikovanje životinja sa G+, G- ili MIX sepsom nije moguće jer je analiza glavnih komponenti (PCA) pokazala sličnu ekspresiju hematoloških i metaboličkih parametara krvi kod životinja bez obzira na tip eksperimentalne sepse (G+, G- ili MIX), uz minimalno odstupanje od životinja sa MIX sepsom od životinja sa sepsom izazvanom čistom kulturom (G+ ili G-). Analiza koeficijenta korelacije pokazala je 507 značajnih korelacija između rutinskih parametara krvi (207 kod EC, 186 kod SA i 114 kod MIX sepse) i neke od njih su u funkciji tipa sepse. Nije moguće razlikovati vrstu sepse u funkciji parametara krvi. Nakon ekstrakcije dve komponente opterećenje po parametrima krvi bilo je slično kod svih vrsta sepse (pozitivno opterećenje različitim vrstama belih krvnih zrnaca, PLT, glukozom, amilazom, Cl, ureom i osmolarnošću, a negativno albuminom, ukupnim proteinom, holesterolom , kreatinin i ALT). Iako su naši rezultati pokazali razliku u vrednostima i koeficijentima korelacije za rutinske biohemijske i hematološke parametre krvi, multiparametarska statistika je pokazala da oni nisu korisno sredstvo za razlikovanje tipa sepse prema bakterijskom uzročniku.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Letopis Naučnih Radova
T1  - Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats
T1  - Analiza glavnih komponenti i korelacije rutinskih parametara krvi u eksperimentalnoj sepsi kod pacova
EP  - 152
IS  - 2
SP  - 143
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1880
ER  - 

@article{
author = "Stojanović, Dragica and Cincović, Marko and Štrbac, Filip and Petrović, Kosta and CRISTINA, ROMEO TEODOR and Kovačević, Zorana",
year = "2021",
abstract = "Sepsis can be defined as a multisystem response of the organism on bacterial infection agents. The aim of this study is to provides an extensive analysis of associations between numerous routine biochemical and hematological markers in function of type of sepsis caused by experimental Gram-positive (G+, Staphylococcus aureus), Gram-negative (G-, Escherichia coli) and mixed (MIX) microorganism in laboratory rats model. In G- and G+ sepsis higher values of erythrocytes, total protein, albumin, creatinine, sodium and plasma osmolarity were noted and lower triglycerides values compared to MIX sepsis. Existence of statistically significant interaction between time and type of sepsis was showed that values of erythrocytes, neutrophiles, LUC, glucose, total protein, urea, Na, Cl, osmolarity and LDH were changed during time in function of sepsis type. Distinguish of animals with G+, G- or MIX sepsis is not possible because principal component analysis (PCA) demonstrated similar expression of blood hematological and metabolic parameters in animals regardless type of experimental sepsis (G+, G- or MIX), with minimal deviation of animal with MIX sepsis from animals with sepsis caused by pure culture (G+ or G-). Correlation coefficient analysis showed 507 significant correlations between routine blood parameters (207 in G-, 186 in G+ and 114 in MIX) and some of them are in function of type of sepsis. Distinguish of type of sepsis in function of blood parameters is not possible. After extraction of two components they load by blood parameters was similar in all type of sepsis (positive load with different type of white blood cells, PLT, glucose, amylase, Cl, urea and osmolarity, and negative load with albumin, total protein, cholesterol, creatinine and ALT). Although our results demonstrated difference in values and correlation coefficients for routine blood biochemistry and hematology parameters, multiparametric statistic showed they not be a useful tool for distinguishing type of sepsis according bacterial causative., Sepsa se može definisati kao multisistemski odgovor organizma na uzročnike bakterijske infekcije. Cilj ove studije je da pruži opsežnu analizu povezanosti brojnih rutinskih biohemijskih i hematoloških markera u funkciji tipa sepse izazvane eksperimentalnim Gram-pozitivnim (G+, Staphylococcus aureus), Gramnegativnim (G-, Escherichia coli) i mešani (MIX) mikroorganizam u modelu laboratorijskih pacova. U G- i G+ sepsi su zabeležene više vrednosti eritrocita, ukupnog proteina, albumina, kreatinina, natrijuma i osmolarnosti plazme i niže vrednosti triglicerida u odnosu na MIX sepsu. Utvrđeno je postojanje statistički značajne interakcije između vremena i tipa sepse da su se vrednosti eritrocita, neutrofila, LUC, glukoze, ukupnog proteina, uree, Na, Cl, osmolarnosti i LDH menjale tokom vremena u funkciji tipa sepse. Razlikovanje životinja sa G+, G- ili MIX sepsom nije moguće jer je analiza glavnih komponenti (PCA) pokazala sličnu ekspresiju hematoloških i metaboličkih parametara krvi kod životinja bez obzira na tip eksperimentalne sepse (G+, G- ili MIX), uz minimalno odstupanje od životinja sa MIX sepsom od životinja sa sepsom izazvanom čistom kulturom (G+ ili G-). Analiza koeficijenta korelacije pokazala je 507 značajnih korelacija između rutinskih parametara krvi (207 kod EC, 186 kod SA i 114 kod MIX sepse) i neke od njih su u funkciji tipa sepse. Nije moguće razlikovati vrstu sepse u funkciji parametara krvi. Nakon ekstrakcije dve komponente opterećenje po parametrima krvi bilo je slično kod svih vrsta sepse (pozitivno opterećenje različitim vrstama belih krvnih zrnaca, PLT, glukozom, amilazom, Cl, ureom i osmolarnošću, a negativno albuminom, ukupnim proteinom, holesterolom , kreatinin i ALT). Iako su naši rezultati pokazali razliku u vrednostima i koeficijentima korelacije za rutinske biohemijske i hematološke parametre krvi, multiparametarska statistika je pokazala da oni nisu korisno sredstvo za razlikovanje tipa sepse prema bakterijskom uzročniku.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Letopis Naučnih Radova",
title = "Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats, Analiza glavnih komponenti i korelacije rutinskih parametara krvi u eksperimentalnoj sepsi kod pacova",
pages = "152-143",
number = "2",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1880"
}

Stojanović, D., Cincović, M., Štrbac, F., Petrović, K., CRISTINA, R. T.,& Kovačević, Z.. (2021). Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats. in Letopis Naučnih Radova
Poljoprivredni fakultet, Univerzitet u Novom Sadu., 45(2), 143-152.
https://hdl.handle.net/21.15107/rcub_rimsi_1880

Stojanović D, Cincović M, Štrbac F, Petrović K, CRISTINA RT, Kovačević Z. Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats. in Letopis Naučnih Radova. 2021;45(2):143-152.
https://hdl.handle.net/21.15107/rcub_rimsi_1880 .

Stojanović, Dragica, Cincović, Marko, Štrbac, Filip, Petrović, Kosta, CRISTINA, ROMEO TEODOR, Kovačević, Zorana, "Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats" in Letopis Naučnih Radova, 45, no. 2 (2021):143-152,
https://hdl.handle.net/21.15107/rcub_rimsi_1880 .

TY  - JOUR
AU  - Petrović, Kosta
AU  - Štrbac, Filip
AU  - Stojanović, Dragica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1887
AB  - Peripartalni period kod krava predstavlja stanje metaboličkog stresa koje se karakteriše različitim negativnim efektima. U ovom radu su opisani inflamatorni procesi kod krava u peripartalnom periodu. Naime, opisano je delovanje citokina, kao i interakcija citokina, metabolita i jetre kao centralnog metaboličkog organa. Prikazana je inflamacija tkiva u peripartalnom periodu sa posebnim osvrtom na značaj faktora nekroze tumora alfa (TNF-α). U tom kontekstu, ispitan je uticaj aplikacije niacina kod krava na regulisanje inflamatornog odgovora kroz merenje vrednosti TNF-α, haptoglobina i fibrinogena i poređenje sa kontrolnom grupom. Smanjenje koncentracije TNF-α i haptoglobina pod uticajem niacina ukazuje na njegov antiinflamatorni efekat, što sugeriše na značaj njegove primene kod krava u peripartalnom periodu.
AB  - The peripartum period in cows represents a state of metabolic stress characterized by various negative effects. This paper describes the inflammatory processes in cows during the peripartum period. Namely, it describes the action of cytokines, as well as the interactions of cytokines, metabolites and the liver as the central metabolic organ. Tissue inflammation in the peripartum period is presented with a particular focus on the importance of tumor necrosis factor alpha (TNF-α). Within this context, the influence of niacin application in cows on the regulation of the inflammatory response was examined by measuring the values of the TNF-α, haptoglobin and fibrinogen and comparing it with the control group. The decrease in the concentration of TNF-α and haptoglobin under the influence of niacin indicates its anti-inflammatory effects, which suggests the importance of its use in cows in the peripartum period.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu
T1  - Influence of Niacin Application on TNF-α, Haptoglobin and Fibrinogen Values in Cows in Peripartal Period
EP  - 32
IS  - 1
SP  - 26
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1887
ER  - 

@article{
author = "Petrović, Kosta and Štrbac, Filip and Stojanović, Dragica",
year = "2021",
abstract = "Peripartalni period kod krava predstavlja stanje metaboličkog stresa koje se karakteriše različitim negativnim efektima. U ovom radu su opisani inflamatorni procesi kod krava u peripartalnom periodu. Naime, opisano je delovanje citokina, kao i interakcija citokina, metabolita i jetre kao centralnog metaboličkog organa. Prikazana je inflamacija tkiva u peripartalnom periodu sa posebnim osvrtom na značaj faktora nekroze tumora alfa (TNF-α). U tom kontekstu, ispitan je uticaj aplikacije niacina kod krava na regulisanje inflamatornog odgovora kroz merenje vrednosti TNF-α, haptoglobina i fibrinogena i poređenje sa kontrolnom grupom. Smanjenje koncentracije TNF-α i haptoglobina pod uticajem niacina ukazuje na njegov antiinflamatorni efekat, što sugeriše na značaj njegove primene kod krava u peripartalnom periodu., The peripartum period in cows represents a state of metabolic stress characterized by various negative effects. This paper describes the inflammatory processes in cows during the peripartum period. Namely, it describes the action of cytokines, as well as the interactions of cytokines, metabolites and the liver as the central metabolic organ. Tissue inflammation in the peripartum period is presented with a particular focus on the importance of tumor necrosis factor alpha (TNF-α). Within this context, the influence of niacin application in cows on the regulation of the inflammatory response was examined by measuring the values of the TNF-α, haptoglobin and fibrinogen and comparing it with the control group. The decrease in the concentration of TNF-α and haptoglobin under the influence of niacin indicates its anti-inflammatory effects, which suggests the importance of its use in cows in the peripartum period.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu, Influence of Niacin Application on TNF-α, Haptoglobin and Fibrinogen Values in Cows in Peripartal Period",
pages = "32-26",
number = "1",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1887"
}

Petrović, K., Štrbac, F.,& Stojanović, D.. (2021). Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 2(1), 26-32.
https://hdl.handle.net/21.15107/rcub_rimsi_1887

Petrović K, Štrbac F, Stojanović D. Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu. in Veterinarski Pregled. 2021;2(1):26-32.
https://hdl.handle.net/21.15107/rcub_rimsi_1887 .

Petrović, Kosta, Štrbac, Filip, Stojanović, Dragica, "Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu" in Veterinarski Pregled, 2, no. 1 (2021):26-32,
https://hdl.handle.net/21.15107/rcub_rimsi_1887 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1881
AB  - Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразитских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације на бази етарских уља већ користе, попут различитих шампона. Међутим, главно ограничење за употребу ових производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП – глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова in vivo ефикасност, као и омогућити контролисано ослобађање. На тај начин се може смањити потребна доза и додатно повећати сигурност примене.
AB  - Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. Although researches have highlighted different possible application of these oils, little is known about their use in animals including dogs and cats. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties such as complex chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain essential oils based formulations are already in use, such as different shampoos. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that are involved in metabolic processes (as UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the methods of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their in vivo efficacy as well as enable controlled release. That can reduce the required dose and additionally increase safety of their application.
PB  - ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука
T2  - Ветеринарски Журнал Републике Српске
T1  - Могућности и ограничења примене етарских уља код паса и мачака
T1  - Possibilities and Limitations of the Use of Essential Oils in Dogs and Cats
EP  - 265
IS  - 1-2
SP  - 238
VL  - XXI
DO  - 10.7251/VETJEN2101238S
ER  - 

@article{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir",
year = "2021",
abstract = "Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразитских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације на бази етарских уља већ користе, попут различитих шампона. Међутим, главно ограничење за употребу ових производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП – глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова in vivo ефикасност, као и омогућити контролисано ослобађање. На тај начин се може смањити потребна доза и додатно повећати сигурност примене., Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. Although researches have highlighted different possible application of these oils, little is known about their use in animals including dogs and cats. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties such as complex chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain essential oils based formulations are already in use, such as different shampoos. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that are involved in metabolic processes (as UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the methods of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their in vivo efficacy as well as enable controlled release. That can reduce the required dose and additionally increase safety of their application.",
publisher = "ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука",
journal = "Ветеринарски Журнал Републике Српске",
title = "Могућности и ограничења примене етарских уља код паса и мачака, Possibilities and Limitations of the Use of Essential Oils in Dogs and Cats",
pages = "265-238",
number = "1-2",
volume = "XXI",
doi = "10.7251/VETJEN2101238S"
}

Štrbac, F., Petrović, K., Stojanović, D.,& Ratajac, R.. (2021). Могућности и ограничења примене етарских уља код паса и мачака. in Ветеринарски Журнал Републике Српске
ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука., XXI(1-2), 238-265.
https://doi.org/10.7251/VETJEN2101238S

Štrbac F, Petrović K, Stojanović D, Ratajac R. Могућности и ограничења примене етарских уља код паса и мачака. in Ветеринарски Журнал Републике Српске. 2021;XXI(1-2):238-265.
doi:10.7251/VETJEN2101238S .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, "Могућности и ограничења примене етарских уља код паса и мачака" in Ветеринарски Журнал Републике Српске, XXI, no. 1-2 (2021):238-265,
https://doi.org/10.7251/VETJEN2101238S . .

TY  - CONF
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Hristovska, Talija
AU  - Štrbac, Filip
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1904
AB  - Cilj ovog rada je da se utvrdi uticaj niacina na metabolizam holesterola i ispita kako se istraživani uticaj odražava na zapaljenske procese i funkcionlani status jetre kod krava u ranoj laktaciji. Ogled je izvršen na 30 krava Holštajn-frizijske rase: 15 koje su primale niacin i 15 krava u negativnoj kontroli. Niacin je aplikovan u periodu dve nedelje pre i dve nedelje posle teljenja. Krv je uzimana venepunkcijom v.coccigea kod krava u periodu pre jutarnjeg hranjenja, u momentu teljenja, potom jednu i dve nedelje nakon teljenja. Aplikacija niacina dovodi do smanjenja koncentracije tumor necrosis factor alpha (TNF-α), haptoglobina i nonesterified fatty acid (NEFA), dok na vrednosti fibrinogena nije pokazao značajan efekat. Aplikacija niacina povećava vrednost holesterola, albumina i smanjuje vrednost bilirubina, što je znak boljeg funkcionalnog stanja hepatocita. Niacin pored antilipolitičkog efekta, pokazuje i antiinflamatorni efekat koji može biti značaj mehanizam u zaštiti hepatocita u ranoj laktaciji kod mlečnih krava. Od velikog značaja se pokazao uticaj niacina na TNF-α, jer ovaj citokin kontroliše korelaciju između lipolize i metabolizma holesterola sa inflamatornim odgovorom, posmatrano kroz indeks funkcionalnog statusa jetre.
AB  - The aim of this study is to determine the effect of niacin on cholesterol metabolism and functional liver status in cows in early lactation. The experiment was performed on 30 Holstein-Friesian cows: 15 receiving niacin and 15 cows in negative control. Niacin was administered for two weeks before and two weeks after calving. Blood was taken by venipuncture of v.coccigea in cows before feeding in the morning, at the time of calving, and then one and two weeks after calving. Niacin administration reduced the concentration of tumor necrosis factor alpha (TNF-α), haptoglobin and nonesterified fatty acid (NEFA), while it showed no significant effect on fibrinogen values. Niacin administration increases cholesterol, albumin, and bilirubin levels, which is a sign of a better functional state of hepatocytes. Niacin, in addition to its antilipolitic effect, also exhibits an anti-inflammatory effect, which may be a significant mechanism in the protection of hepatocytes in early lactation in dairy cows. The influence of niacin on TNF-α has been shown to be of great importance, as this cytokine controls the correlation between lipolysis and cholesterol metabolism with an inflammatory response, viewed through an index of liver functional status.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)
C3  - Zbornik radova Zdravstvena zaštita i reprodukcija životinja
T1  - Uticaj aplikacije niacina na razvoj inflamacije i metabolizam holesterola kod krava u ranoj laktaciji
T1  - The Effect of Niacin Application on the Development of Inflammation and Cholesterol Metabolism in Cows in Early Lactation
EP  - 110
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1904
ER  - 

@conference{
author = "Petrović, Kosta and Stojanović, Dragica and Hristovska, Talija and Štrbac, Filip",
year = "2020",
abstract = "Cilj ovog rada je da se utvrdi uticaj niacina na metabolizam holesterola i ispita kako se istraživani uticaj odražava na zapaljenske procese i funkcionlani status jetre kod krava u ranoj laktaciji. Ogled je izvršen na 30 krava Holštajn-frizijske rase: 15 koje su primale niacin i 15 krava u negativnoj kontroli. Niacin je aplikovan u periodu dve nedelje pre i dve nedelje posle teljenja. Krv je uzimana venepunkcijom v.coccigea kod krava u periodu pre jutarnjeg hranjenja, u momentu teljenja, potom jednu i dve nedelje nakon teljenja. Aplikacija niacina dovodi do smanjenja koncentracije tumor necrosis factor alpha (TNF-α), haptoglobina i nonesterified fatty acid (NEFA), dok na vrednosti fibrinogena nije pokazao značajan efekat. Aplikacija niacina povećava vrednost holesterola, albumina i smanjuje vrednost bilirubina, što je znak boljeg funkcionalnog stanja hepatocita. Niacin pored antilipolitičkog efekta, pokazuje i antiinflamatorni efekat koji može biti značaj mehanizam u zaštiti hepatocita u ranoj laktaciji kod mlečnih krava. Od velikog značaja se pokazao uticaj niacina na TNF-α, jer ovaj citokin kontroliše korelaciju između lipolize i metabolizma holesterola sa inflamatornim odgovorom, posmatrano kroz indeks funkcionalnog statusa jetre., The aim of this study is to determine the effect of niacin on cholesterol metabolism and functional liver status in cows in early lactation. The experiment was performed on 30 Holstein-Friesian cows: 15 receiving niacin and 15 cows in negative control. Niacin was administered for two weeks before and two weeks after calving. Blood was taken by venipuncture of v.coccigea in cows before feeding in the morning, at the time of calving, and then one and two weeks after calving. Niacin administration reduced the concentration of tumor necrosis factor alpha (TNF-α), haptoglobin and nonesterified fatty acid (NEFA), while it showed no significant effect on fibrinogen values. Niacin administration increases cholesterol, albumin, and bilirubin levels, which is a sign of a better functional state of hepatocytes. Niacin, in addition to its antilipolitic effect, also exhibits an anti-inflammatory effect, which may be a significant mechanism in the protection of hepatocytes in early lactation in dairy cows. The influence of niacin on TNF-α has been shown to be of great importance, as this cytokine controls the correlation between lipolysis and cholesterol metabolism with an inflammatory response, viewed through an index of liver functional status.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)",
journal = "Zbornik radova Zdravstvena zaštita i reprodukcija životinja",
title = "Uticaj aplikacije niacina na razvoj inflamacije i metabolizam holesterola kod krava u ranoj laktaciji, The Effect of Niacin Application on the Development of Inflammation and Cholesterol Metabolism in Cows in Early Lactation",
pages = "110-105",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1904"
}

Petrović, K., Stojanović, D., Hristovska, T.,& Štrbac, F.. (2020). Uticaj aplikacije niacina na razvoj inflamacije i metabolizam holesterola kod krava u ranoj laktaciji. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja
Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)., 105-110.
https://hdl.handle.net/21.15107/rcub_rimsi_1904

Petrović K, Stojanović D, Hristovska T, Štrbac F. Uticaj aplikacije niacina na razvoj inflamacije i metabolizam holesterola kod krava u ranoj laktaciji. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja. 2020;:105-110.
https://hdl.handle.net/21.15107/rcub_rimsi_1904 .

Petrović, Kosta, Stojanović, Dragica, Hristovska, Talija, Štrbac, Filip, "Uticaj aplikacije niacina na razvoj inflamacije i metabolizam holesterola kod krava u ranoj laktaciji" in Zbornik radova Zdravstvena zaštita i reprodukcija životinja (2020):105-110,
https://hdl.handle.net/21.15107/rcub_rimsi_1904 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1907
AB  - Нерационална примена комерцијалних антихелминтика је довела до развоја антихелминтичке резистенције (АР) код многих врста ендопаразита домаћих животиња. То је довело до смањења ефикасности лекова из група бензимидазола, имидазотиазола и макроцикличних лактона и, последично, до великих економских губитака. Због тога је један од најважнијих приоритета модерног сточарства борба против АР, а која укључује примену адекватних in vitro и in vivo метода за њену детекцију. In vitro методе се заснивају на испитивању утицаја различитих концентрација одређене активне супстанце на одређене изоловане стадијуме паразита у лабораторијским условима. Међу њима су најосетљивије и најчешће коришћење методе тзв. egg hatch assay (EHA) и larval development assay (LDA). Са друге стране, in vivo методе као што је тзв. faecal egg count reduction test (FECRT), подразумевају испитивање утицаја препарата на паразите у самим животињима у теренским условима, где се ефикасност неког лека мери запаженом редукцијом броја одређених паразитских облика након третмана. Свака од наведених метода има своје предности и недостатке, због чега постоје стални напори да се усавршаве и стандардизују. У последње време се у контексту детекције АР све више испитују и користе различите молекуларне технике као што су PCR и пиросеквенцирање. Такође, важно је напоменути да се in vitro и in vivo тестови могу користити и за откривање нових супстанци као што су нека природна једињења са антихелминтичким потенцијалом, као и за испитивање њихове ефикасности. Из свега наведеног се може закључити да наведене методе имају вишеструки значај у борби против антихелминтичке резистенције.
AB  - The irrational use of commercial anthelmintics has led to the development of anthelmintic resistance (AR) in many species of endoparasites commonly found in domestic animals. This has led to a decrease in the efficiency of drugs from the groups of benzimidazoles, imidazothiazoles and macrocyclic lactones and, consequently, to major economic losses. Therefore, one of the most important priorities of modern animal husbandry is to combat AR, which includes the application of adequate in vitro and in vivo methods for its detection. In vitro methods are based on examining the influence of different concentrations of a certain active substance on certain isolated parasitic stages in lab conditions. Among them, the most sensitive and the most common used methods are the egg hatch assay (EHA) and the larval development assay (LDA). On the other hand, in vivo methods such as the faecal egg count reduction test (FECRT), examine the effect of drug preparations on parasite-infected animals in field conditions, whereby the efficiency of a drug is measured by the observed reduction in the number of certain parasitic forms post-treatment. Each of these methods has its advantages and disadvantages, and efforts are regularly made to improve and standardize them. Recently, in the context of AR detection, various molecular techniques such as PCR and pyrosequencing have been increasingly examined and used. It is also important to note that in vitro and in vivo tests can also be used to detect new substances such as certain natural compounds with anthelmintic potential, and can be simultaneously used to test their efficiency. From the above-mentioned points, it can be concluded that these methods have multiple significance in combating anthelmintic resistance.
PB  - Српско ветеринарско друштво
C3  - Зборник радова и кратких садржаја 31. Саветовања ветеринара Србије
T1  - Преглед најважнијих метода за испитивање ефикасности антихелминтика и детекцију антихелминтичке резистенције
T1  - An Overview of the Most Important Methods for Examination of the Efficacy of Anthelmintics and Detection of Anthelmintic Resistance
EP  - 224
SP  - 219
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1907
ER  - 

@conference{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica",
year = "2020",
abstract = "Нерационална примена комерцијалних антихелминтика је довела до развоја антихелминтичке резистенције (АР) код многих врста ендопаразита домаћих животиња. То је довело до смањења ефикасности лекова из група бензимидазола, имидазотиазола и макроцикличних лактона и, последично, до великих економских губитака. Због тога је један од најважнијих приоритета модерног сточарства борба против АР, а која укључује примену адекватних in vitro и in vivo метода за њену детекцију. In vitro методе се заснивају на испитивању утицаја различитих концентрација одређене активне супстанце на одређене изоловане стадијуме паразита у лабораторијским условима. Међу њима су најосетљивије и најчешће коришћење методе тзв. egg hatch assay (EHA) и larval development assay (LDA). Са друге стране, in vivo методе као што је тзв. faecal egg count reduction test (FECRT), подразумевају испитивање утицаја препарата на паразите у самим животињима у теренским условима, где се ефикасност неког лека мери запаженом редукцијом броја одређених паразитских облика након третмана. Свака од наведених метода има своје предности и недостатке, због чега постоје стални напори да се усавршаве и стандардизују. У последње време се у контексту детекције АР све више испитују и користе различите молекуларне технике као што су PCR и пиросеквенцирање. Такође, важно је напоменути да се in vitro и in vivo тестови могу користити и за откривање нових супстанци као што су нека природна једињења са антихелминтичким потенцијалом, као и за испитивање њихове ефикасности. Из свега наведеног се може закључити да наведене методе имају вишеструки значај у борби против антихелминтичке резистенције., The irrational use of commercial anthelmintics has led to the development of anthelmintic resistance (AR) in many species of endoparasites commonly found in domestic animals. This has led to a decrease in the efficiency of drugs from the groups of benzimidazoles, imidazothiazoles and macrocyclic lactones and, consequently, to major economic losses. Therefore, one of the most important priorities of modern animal husbandry is to combat AR, which includes the application of adequate in vitro and in vivo methods for its detection. In vitro methods are based on examining the influence of different concentrations of a certain active substance on certain isolated parasitic stages in lab conditions. Among them, the most sensitive and the most common used methods are the egg hatch assay (EHA) and the larval development assay (LDA). On the other hand, in vivo methods such as the faecal egg count reduction test (FECRT), examine the effect of drug preparations on parasite-infected animals in field conditions, whereby the efficiency of a drug is measured by the observed reduction in the number of certain parasitic forms post-treatment. Each of these methods has its advantages and disadvantages, and efforts are regularly made to improve and standardize them. Recently, in the context of AR detection, various molecular techniques such as PCR and pyrosequencing have been increasingly examined and used. It is also important to note that in vitro and in vivo tests can also be used to detect new substances such as certain natural compounds with anthelmintic potential, and can be simultaneously used to test their efficiency. From the above-mentioned points, it can be concluded that these methods have multiple significance in combating anthelmintic resistance.",
publisher = "Српско ветеринарско друштво",
journal = "Зборник радова и кратких садржаја 31. Саветовања ветеринара Србије",
title = "Преглед најважнијих метода за испитивање ефикасности антихелминтика и детекцију антихелминтичке резистенције, An Overview of the Most Important Methods for Examination of the Efficacy of Anthelmintics and Detection of Anthelmintic Resistance",
pages = "224-219",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1907"
}

Štrbac, F., Petrović, K.,& Stojanović, D.. (2020). Преглед најважнијих метода за испитивање ефикасности антихелминтика и детекцију антихелминтичке резистенције. in Зборник радова и кратких садржаја 31. Саветовања ветеринара Србије
Српско ветеринарско друштво., 219-224.
https://hdl.handle.net/21.15107/rcub_rimsi_1907

Štrbac F, Petrović K, Stojanović D. Преглед најважнијих метода за испитивање ефикасности антихелминтика и детекцију антихелминтичке резистенције. in Зборник радова и кратких садржаја 31. Саветовања ветеринара Србије. 2020;:219-224.
https://hdl.handle.net/21.15107/rcub_rimsi_1907 .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, "Преглед најважнијих метода за испитивање ефикасности антихелминтика и детекцију антихелминтичке резистенције" in Зборник радова и кратких садржаја 31. Саветовања ветеринара Србије (2020):219-224,
https://hdl.handle.net/21.15107/rcub_rimsi_1907 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1910
AB  - Промене у концентрацији укупних протеина, као и појединачних концентрација албумина и глобулина у крвној плазми често указују на присуство одређених обољења код птица. Међутим, до ових промена може доћи и услед дејства различитих физиолошких фактора, због чега их је неопходно идентификовати и испитати њихов утицај. Циљ овог истраживања
је био испитати промену наведених биохемијских параметара у зависности од узраста пилића током производног циклуса (1-42. дан). За овај оглед су коришћени товни хибриди пилића (н=90) који су подељени у три групе у зависности од испитиваног параметра. Унутар сваке групе пилићи су подељени у неколико подгрупа у зависности од узраста (1., 3., 7., 14., 21. и 42. дан). Сви биохемијски параметри су одређивани квантитативном спектрофотометријском методом у лабораторији за биохемију на Пољопривредном факултету у Новом Саду. Раст концентрације албумина је био статистички значајан (п<0,05) између 1. дана (17,41 г/Л) и 3. дана (20,10 г/Л), и наставио се до 14. дана (21,58 г/Л), након чега је стагнирао до краја производног циклуса са забележеном вредношћу од 21,47 г/Л 42. дана. Значајан раст концентрације албумина у прва три дана живота се може објаснити повећаном синтезом албумина у јетри у том периоду. Са друге стране, услед губитка наслеђених имуноглобулина, концентрација глобулина је опадала до 14. дана (26,87 г/Л), такође статистички значајно (п<0,05) у прва три дана (30,42-28,25 г/Л), да би након 14. дана уследио пораст до краја производног циклуса (31,47 г/Л 42. дана) услед продукције антитела од стране пилића. Због оваквих промена у концентрацијама поменутих фракција, концентрација укупних протеина је током целог производног циклуса била у благом порасту (47,83-52,93 г/Л). На основу добијених резултата се може закључити да је узраст један од фактора који утиче на протеински систем крвне плазме птица, што се мора узети у обзир приликом тумачења резултата у свакодневној клиничкој пракси.
AB  - Changes in the total protein concentration, as well as changes in individual concentrations of albumin and globulin in blood plasma often indicate the presence of certain diseases in birds. However, these changes can also occur due to various physiological factors, which is why they need to be identified and why their influence must be examined. The aim of this study was to examine the change in these biochemical parameters depending on the age of the chickens during the production cycle (day 1-42). Fattened hybrids of chickens (n=90) were used for this experiment, which were divided into three groups depending on the selected parameter. Within each group, the chickens were divided into multiple subgroups depending on age (the 1st, 3rd, 7th, 14th, 21st and 42nd day of age). All biochemical parameters were determined by the quantitative spectrophotometric method in the laboratory of biochemistry at the Faculty of Agriculture in Novi Sad. The increase of the albumin concentration was statistically significant (p<0.05) between day 1 (17.41 g/L) and day 3 (20.10 g/L), and continued to rise up to day 14 (21.58 g/L), after which it entered stagnation by the end of the production cycle with a recorded value of 21.47 g/L on day 42. The significant increase in albumin concentration in the first three days can be explained by the
increased synthesis of albumin in the liver during that period. In comparison, due to the loss of inherited immunoglobulin, the globulin concentration was decreasing up to day 14 (26.87 g/L), also at a statistically significant rate (p<0.05) in the first three days (30.42-28.25 g/L). That was followed by an increase up to the end of the production cycle after day 14 (31.47 g/L on day 42) due to the
production of antibodies by the chickens. Due to such changes in the concentrations of the mentioned fractions, the total protein concentration was slightly increasing during the entire production cycle (47.83-52.93 g/L). Based on the obtained results, it can be concluded that age is one of the factors which influence the protein system of avian blood plasma. As such, it must be taken into account when interpreting results in everyday clinical practice.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник кратких садржаја 25. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Утицај узраста пилића на концентрацију укупних протеина, албумина и глобулина у крвној плазми
T1  - The Effect of Age on the Concentration of Total Protein, Albumin and Globulin in the Blood Plasma of Chickens
EP  - 151
SP  - 150
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1910
ER  - 

@conference{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica",
year = "2020",
abstract = "Промене у концентрацији укупних протеина, као и појединачних концентрација албумина и глобулина у крвној плазми често указују на присуство одређених обољења код птица. Међутим, до ових промена може доћи и услед дејства различитих физиолошких фактора, због чега их је неопходно идентификовати и испитати њихов утицај. Циљ овог истраживања
је био испитати промену наведених биохемијских параметара у зависности од узраста пилића током производног циклуса (1-42. дан). За овај оглед су коришћени товни хибриди пилића (н=90) који су подељени у три групе у зависности од испитиваног параметра. Унутар сваке групе пилићи су подељени у неколико подгрупа у зависности од узраста (1., 3., 7., 14., 21. и 42. дан). Сви биохемијски параметри су одређивани квантитативном спектрофотометријском методом у лабораторији за биохемију на Пољопривредном факултету у Новом Саду. Раст концентрације албумина је био статистички значајан (п<0,05) између 1. дана (17,41 г/Л) и 3. дана (20,10 г/Л), и наставио се до 14. дана (21,58 г/Л), након чега је стагнирао до краја производног циклуса са забележеном вредношћу од 21,47 г/Л 42. дана. Значајан раст концентрације албумина у прва три дана живота се може објаснити повећаном синтезом албумина у јетри у том периоду. Са друге стране, услед губитка наслеђених имуноглобулина, концентрација глобулина је опадала до 14. дана (26,87 г/Л), такође статистички значајно (п<0,05) у прва три дана (30,42-28,25 г/Л), да би након 14. дана уследио пораст до краја производног циклуса (31,47 г/Л 42. дана) услед продукције антитела од стране пилића. Због оваквих промена у концентрацијама поменутих фракција, концентрација укупних протеина је током целог производног циклуса била у благом порасту (47,83-52,93 г/Л). На основу добијених резултата се може закључити да је узраст један од фактора који утиче на протеински систем крвне плазме птица, што се мора узети у обзир приликом тумачења резултата у свакодневној клиничкој пракси., Changes in the total protein concentration, as well as changes in individual concentrations of albumin and globulin in blood plasma often indicate the presence of certain diseases in birds. However, these changes can also occur due to various physiological factors, which is why they need to be identified and why their influence must be examined. The aim of this study was to examine the change in these biochemical parameters depending on the age of the chickens during the production cycle (day 1-42). Fattened hybrids of chickens (n=90) were used for this experiment, which were divided into three groups depending on the selected parameter. Within each group, the chickens were divided into multiple subgroups depending on age (the 1st, 3rd, 7th, 14th, 21st and 42nd day of age). All biochemical parameters were determined by the quantitative spectrophotometric method in the laboratory of biochemistry at the Faculty of Agriculture in Novi Sad. The increase of the albumin concentration was statistically significant (p<0.05) between day 1 (17.41 g/L) and day 3 (20.10 g/L), and continued to rise up to day 14 (21.58 g/L), after which it entered stagnation by the end of the production cycle with a recorded value of 21.47 g/L on day 42. The significant increase in albumin concentration in the first three days can be explained by the
increased synthesis of albumin in the liver during that period. In comparison, due to the loss of inherited immunoglobulin, the globulin concentration was decreasing up to day 14 (26.87 g/L), also at a statistically significant rate (p<0.05) in the first three days (30.42-28.25 g/L). That was followed by an increase up to the end of the production cycle after day 14 (31.47 g/L on day 42) due to the
production of antibodies by the chickens. Due to such changes in the concentrations of the mentioned fractions, the total protein concentration was slightly increasing during the entire production cycle (47.83-52.93 g/L). Based on the obtained results, it can be concluded that age is one of the factors which influence the protein system of avian blood plasma. As such, it must be taken into account when interpreting results in everyday clinical practice.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник кратких садржаја 25. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Утицај узраста пилића на концентрацију укупних протеина, албумина и глобулина у крвној плазми, The Effect of Age on the Concentration of Total Protein, Albumin and Globulin in the Blood Plasma of Chickens",
pages = "151-150",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1910"
}

Štrbac, F., Petrović, K.,& Stojanović, D.. (2020). Утицај узраста пилића на концентрацију укупних протеина, албумина и глобулина у крвној плазми. in Зборник кратких садржаја 25. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 150-151.
https://hdl.handle.net/21.15107/rcub_rimsi_1910

Štrbac F, Petrović K, Stojanović D. Утицај узраста пилића на концентрацију укупних протеина, албумина и глобулина у крвној плазми. in Зборник кратких садржаја 25. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2020;:150-151.
https://hdl.handle.net/21.15107/rcub_rimsi_1910 .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, "Утицај узраста пилића на концентрацију укупних протеина, албумина и глобулина у крвној плазми" in Зборник кратких садржаја 25. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2020):150-151,
https://hdl.handle.net/21.15107/rcub_rimsi_1910 .