TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Rinaldi, Laura
AU  - Ciccone, Elena
AU  - Maurelli, Maria Paola
AU  - Cringoli, Giuseppe
AU  - Bosco, Antonio
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2105
AB  - Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.
PB  - Frontiers
T2  - Frontiers in Veterinary Science
T1  - In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1232570
VL  - 10
DO  - 10.3389/fvets.2023.1232570
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Rinaldi, Laura and Ciccone, Elena and Maurelli, Maria Paola and Cringoli, Giuseppe and Bosco, Antonio",
year = "2023",
abstract = "Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.",
publisher = "Frontiers",
journal = "Frontiers in Veterinary Science",
title = "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1232570",
volume = "10",
doi = "10.3389/fvets.2023.1232570"
}

Štrbac, F., Krnjajić, S., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Rinaldi, L., Ciccone, E., Maurelli, M. P., Cringoli, G.,& Bosco, A.. (2023). In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science
Frontiers., 10(1232570).
https://doi.org/10.3389/fvets.2023.1232570

Štrbac F, Krnjajić S, Stojanović D, Ratajac R, Simin N, Orčić D, Rinaldi L, Ciccone E, Maurelli MP, Cringoli G, Bosco A. In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science. 2023;10(1232570).
doi:10.3389/fvets.2023.1232570 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Rinaldi, Laura, Ciccone, Elena, Maurelli, Maria Paola, Cringoli, Giuseppe, Bosco, Antonio, "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Frontiers in Veterinary Science, 10, no. 1232570 (2023),
https://doi.org/10.3389/fvets.2023.1232570 . .