TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3188
AB  - Гастроинтестиналне нематоде представљају веома значајне паразите домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анорексије, анемије, јаке дијареје и губитка протеина што све заједно може довести и до угинућа. Комерцијални лекови попут бензимидазола и макроцикличних лактона су се деценијама са успехом користили за контролу ових паразита. Међутим, нерационална примена ових лекова је довела до развоја антихелминтичке резистенције, опадања њихове ефикасности и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, а присутан је и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а у циљу успостављања интегрисаног приступа који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани, циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно отпорних на нематоде, рационалног управљања пашњацима, избалансиране исхране уз допунска хранива, биолошке контроле (употреба гљива, бактерија и слично), развоја вакцина као и употребе ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља). Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће успорити развој и ширење антихелминтичке резистенције, као и постићи одрживу контрола ових паразита и смањење економских губитака.
AB  - Gastrointestinal nematodes are very important parasites of domestic animals, especially in small ruminants. In sheep, diseases usually have a subclinical form with a decrease in body weight and production, and sometimes there can be visible clinical symptoms such as anorexia, anemia, severe diarrhea and protein loss, all of which together can lead to death. Commercial drugs such as benzimidazole and macrocyclic lactones have been successfully used for decades to control these parasites. However, the irrational application of these drugs has led to the development of anthelmintic resistance, a decrease in their effectiveness and consequent economic losses that threaten the sustainability of sheep breeding. In addition, the use of commercial preparations is also associated with the residues in animal products and the environment, and there is also an increase in the price of these preparations. For this reason, novel strategies for controlling of these parasites are actively seeking, with the goal of establishing an integrated approach that involves the application of several different strategies. On the one hand, these include strategies for the rational application of commercial preparations based on refugia (targeted treatments, targeted selective treatments), as well as the combination and rotation of anthelmintics from different chemical groups. On the other hand, an integrated approach implies the use of different alternative strategies such as genetic selection of animals naturally resistant to nematodes, rational management of pastures, balanced nutrition with supplementary nutrients, biological control (use of fungi, bacteria, etc.), development of vaccines as well as the use of botanical preparations (plants and their products such as extracts and essential oils). Various studies have proven the effectiveness of the mentioned strategies in the control of gastrointestinal nematodes of sheep, whereby the best results are achieved by their combined application. Thus, it is possible to slow down the development and spread of anthelmintic resistance, as well as achieve sustainable control of these parasites and reduce economic losses.
PB  - ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука
T2  - Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska
T1  - Савремене стратегије контроле гастроинтестиналних нематода код оваца
T1  - Novel Strategies for the Control of Gastrointestinal Nematodes in Sheep
EP  - 245
IS  - 1-2
SP  - 209
VL  - XXIII
DO  - 10.7251/VETJSR2301209S
DO  - 10.7251/VETJEN2301227S
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir",
year = "2023",
abstract = "Гастроинтестиналне нематоде представљају веома значајне паразите домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анорексије, анемије, јаке дијареје и губитка протеина што све заједно може довести и до угинућа. Комерцијални лекови попут бензимидазола и макроцикличних лактона су се деценијама са успехом користили за контролу ових паразита. Међутим, нерационална примена ових лекова је довела до развоја антихелминтичке резистенције, опадања њихове ефикасности и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, а присутан је и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а у циљу успостављања интегрисаног приступа који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани, циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно отпорних на нематоде, рационалног управљања пашњацима, избалансиране исхране уз допунска хранива, биолошке контроле (употреба гљива, бактерија и слично), развоја вакцина као и употребе ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља). Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће успорити развој и ширење антихелминтичке резистенције, као и постићи одрживу контрола ових паразита и смањење економских губитака., Gastrointestinal nematodes are very important parasites of domestic animals, especially in small ruminants. In sheep, diseases usually have a subclinical form with a decrease in body weight and production, and sometimes there can be visible clinical symptoms such as anorexia, anemia, severe diarrhea and protein loss, all of which together can lead to death. Commercial drugs such as benzimidazole and macrocyclic lactones have been successfully used for decades to control these parasites. However, the irrational application of these drugs has led to the development of anthelmintic resistance, a decrease in their effectiveness and consequent economic losses that threaten the sustainability of sheep breeding. In addition, the use of commercial preparations is also associated with the residues in animal products and the environment, and there is also an increase in the price of these preparations. For this reason, novel strategies for controlling of these parasites are actively seeking, with the goal of establishing an integrated approach that involves the application of several different strategies. On the one hand, these include strategies for the rational application of commercial preparations based on refugia (targeted treatments, targeted selective treatments), as well as the combination and rotation of anthelmintics from different chemical groups. On the other hand, an integrated approach implies the use of different alternative strategies such as genetic selection of animals naturally resistant to nematodes, rational management of pastures, balanced nutrition with supplementary nutrients, biological control (use of fungi, bacteria, etc.), development of vaccines as well as the use of botanical preparations (plants and their products such as extracts and essential oils). Various studies have proven the effectiveness of the mentioned strategies in the control of gastrointestinal nematodes of sheep, whereby the best results are achieved by their combined application. Thus, it is possible to slow down the development and spread of anthelmintic resistance, as well as achieve sustainable control of these parasites and reduce economic losses.",
publisher = "ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука",
journal = "Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska",
title = "Савремене стратегије контроле гастроинтестиналних нематода код оваца, Novel Strategies for the Control of Gastrointestinal Nematodes in Sheep",
pages = "245-209",
number = "1-2",
volume = "XXIII",
doi = "10.7251/VETJSR2301209S, 10.7251/VETJEN2301227S"
}

Štrbac, F., Krnjajić, S., Stojanović, D.,& Ratajac, R.. (2023). Савремене стратегије контроле гастроинтестиналних нематода код оваца. in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska
ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука., XXIII(1-2), 209-245.
https://doi.org/10.7251/VETJSR2301209S

Štrbac F, Krnjajić S, Stojanović D, Ratajac R. Савремене стратегије контроле гастроинтестиналних нематода код оваца. in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska. 2023;XXIII(1-2):209-245.
doi:10.7251/VETJSR2301209S .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, "Савремене стратегије контроле гастроинтестиналних нематода код оваца" in Ветеринарски журнал Републике Српске / Veterinary Journal of Republic of Srpska, XXIII, no. 1-2 (2023):209-245,
https://doi.org/10.7251/VETJSR2301209S . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2002
AB  - Гастроинтестиналне нематоде представљају веома важне паразите код домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анемије, јаке дијареје, анорексије и губитака протеина што све заједно може довести и до угинућа. Комерцијални препарати попут бензимидазола и макроцикличних
лактона су се деценијама са успехом користили у контроли ових паразита. Међутим, њихова нерационална примена је довела до развоја антихелминтичке резистенције, опадања ефикасности лекова и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, при чему је присутан и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а које се ослањају на интегрисан приступ који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани и циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са
друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно резистентних на нематоде, рационално управљање пашњацима, избалансирану исхрану уз допунска хранива, биолошку контролу (употребу гљива, бактерија и слично), развој вакцина као и употребу ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља).
Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће постићи успоравање развоја и ширења антихелминтичке резистенције, као и одрживу контролу ових паразита и смањење економских губитака.
AB  - Gastrointestinal nematodes represent very important parasites in domestic
animals, especially in small ruminants. In sheep, diseases usually have a
subclinical course with a decrease in body weight and production, whereby
sometimes visible clinical symptoms such as anaemia, severe diarrhea, anorexia
and protein loss with a possible fatal outcome may occur. Commercial drugs such
as benzimidasoles and macrocyclic lactones have been used successfully for
decades in the control of these parasites. However, their inappropriate use has led
to the development of anthelmintic resistance, decreasing efficacy of drugs and
consequent economic losses that endanger the sustainability of sheep farming. In
addition, application of commercial preparations is also associated with the
residues in animal products and the environment, while the increase in their price
is also present. Therefore, novel strategies for the control of these parasites are
actively sought, which rely on an integrated approach that involves the application
of several different strategies. On the one hand, these include strategies for the
rational application of commercial drugs based on refugia (target treatments and
target selective treatments), as well as the combination and rotation of
anthelmintics from different chemical groups. On the other hand, an integrated
approach implies the use of various alternative strategies, such as genetic
selection of animals naturally resistant to nematodes, rational management of
pastures, balanced nutrition with supplementary nutrients, biological control (use
of fungi, bacteria, etc.), development of vaccines, as well as the use of botanical
preparations (plants and their products such as extracts and essential oils).
Various studies have proven the effectiveness of the mentioned strategies in the
control of gastrointestinal nematodes of sheep, whereby the best results are
achieved by their combined application. In this way, it is possible to slow down
the development and spread of anthelmintic resistance, and to achieve sustainable
control of these parasites and reduction of economic losses.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле
T1  - GASTROINTESTINAL NEMATODES IN SHEEP - CURRENT SITUATION AND MODERN CONTROL STRATEGIES
EP  - 40
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2002
ER  - 

@conference{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir",
year = "2023",
abstract = "Гастроинтестиналне нематоде представљају веома важне паразите код домаћих животиња, нарочито код малих преживара. Код оваца обољења најчешће имају субклиничан ток са смањењем телесне масе и производње, а понекад може доћи и до видљивих клиничких симптома попут анемије, јаке дијареје, анорексије и губитака протеина што све заједно може довести и до угинућа. Комерцијални препарати попут бензимидазола и макроцикличних
лактона су се деценијама са успехом користили у контроли ових паразита. Међутим, њихова нерационална примена је довела до развоја антихелминтичке резистенције, опадања ефикасности лекова и последичних економских губитака који угрожавају одрживост гајења оваца. Поред тога, примена комерцијалних препарата је повезана и са резидуама које остављају у животињским производима и животној средини, при чему је присутан и раст цене ових препарата. Због тога се активно трага за новијим стратегијама за контролу ових паразита, а које се ослањају на интегрисан приступ који подразумева примену више различитих стратегија. Са једне стране, ту спадају стратегије рационалне примене комерцијалних препарата заснованих на рефугији (циљани третмани и циљани селективни третмани), као и комбинација и ротација антихелминтика из различитих хемијских група. Са
друге стране, интегрисан приступ подразумева и употребу различитих алтернативних стратегија попут генетске селекције животиња природно резистентних на нематоде, рационално управљање пашњацима, избалансирану исхрану уз допунска хранива, биолошку контролу (употребу гљива, бактерија и слично), развој вакцина као и употребу ботаничких препарата (биљака и њихових производа попут екстракта и етарских уља).
Различита истраживања су доказала ефикасност наведених стратегија у контроли гастроинтестиналних нематода оваца, при чему се најбољи резултати постижу њиховом комбинованом применом. На овај начин је могуће постићи успоравање развоја и ширења антихелминтичке резистенције, као и одрживу контролу ових паразита и смањење економских губитака., Gastrointestinal nematodes represent very important parasites in domestic
animals, especially in small ruminants. In sheep, diseases usually have a
subclinical course with a decrease in body weight and production, whereby
sometimes visible clinical symptoms such as anaemia, severe diarrhea, anorexia
and protein loss with a possible fatal outcome may occur. Commercial drugs such
as benzimidasoles and macrocyclic lactones have been used successfully for
decades in the control of these parasites. However, their inappropriate use has led
to the development of anthelmintic resistance, decreasing efficacy of drugs and
consequent economic losses that endanger the sustainability of sheep farming. In
addition, application of commercial preparations is also associated with the
residues in animal products and the environment, while the increase in their price
is also present. Therefore, novel strategies for the control of these parasites are
actively sought, which rely on an integrated approach that involves the application
of several different strategies. On the one hand, these include strategies for the
rational application of commercial drugs based on refugia (target treatments and
target selective treatments), as well as the combination and rotation of
anthelmintics from different chemical groups. On the other hand, an integrated
approach implies the use of various alternative strategies, such as genetic
selection of animals naturally resistant to nematodes, rational management of
pastures, balanced nutrition with supplementary nutrients, biological control (use
of fungi, bacteria, etc.), development of vaccines, as well as the use of botanical
preparations (plants and their products such as extracts and essential oils).
Various studies have proven the effectiveness of the mentioned strategies in the
control of gastrointestinal nematodes of sheep, whereby the best results are
achieved by their combined application. In this way, it is possible to slow down
the development and spread of anthelmintic resistance, and to achieve sustainable
control of these parasites and reduction of economic losses.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле, GASTROINTESTINAL NEMATODES IN SHEEP - CURRENT SITUATION AND MODERN CONTROL STRATEGIES",
pages = "40-39",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2002"
}

Štrbac, F., Krnjajić, S., Stojanović, D.,& Ratajac, R.. (2023). Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле. in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 39-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2002

Štrbac F, Krnjajić S, Stojanović D, Ratajac R. Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле. in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2023;:39-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2002 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, "Гастроинтестиналне нематоде код оваца - актуелна ситуација и савремене стратегије контроле" in Зборник радова и кратких садржаја 28. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2023):39-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2002 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stanković, Slađan
AU  - Tomić, Vedran
AU  - Ratajac, Radomir
AU  - Stojanović, Dragica
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2441
AB  - Gastrointestinal nematodes represent parasites of great importance in veterinary medicine, especially in grazing animals such as small ruminants. In most cases, these parasites cause subclinical diseases with losses in body weight, reduced feed intake and decreased production. However, high worm burdens may lead to severe clinical signs such as anorexia, anaemia, diarrhoea, protein loss, oedema, decreased immunity and fertility, which can lead to fatal outcomes. Therefore, gastrointestinal nematodes hinder sheep farming in different ways leading to high economic losses that are estimated at several hundred million euros in Europe, although it is difficult to quantify it precisely. An additional problem is the development of anthelmintic resistance in nematodes that occur as a result of irrational use of commercial drugs, whereby the main risk factors are overfrequent treatments, underdosing, the use of only one drug without combination or rotation etc. This leads to a decrease in their effectiveness, and consequently to even higher economic losses (an additional 35-40 million euros). Moreover, these costs will tend to increase in the future due to widespread of resistance and the occurrence of multiresistant strains. For these reasons, the focus of nowadays research is finding alternative solutions including genetic selection of naturally resistant animals, pasture management, dietary manipulation, biological control (use of nematophagous fungi, bacteria or earthworms and dung beetles) and the use of plant-based formulations such as extracts and essential oils. All of the above strategies have shown promise, although it appears that none of them could be used independently. Therefore, future strategies for the control of gastrointestinal nematodes should be based on an integrated approach. This implies the combination of mentioned alternatives with rational use of anthelmintics based on refugia strategies (target treatments and target selective treatments).
PB  - Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina
C3  - Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina
T1  - The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2441
ER  - 

@conference{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stanković, Slađan and Tomić, Vedran and Ratajac, Radomir and Stojanović, Dragica",
year = "2023",
abstract = "Gastrointestinal nematodes represent parasites of great importance in veterinary medicine, especially in grazing animals such as small ruminants. In most cases, these parasites cause subclinical diseases with losses in body weight, reduced feed intake and decreased production. However, high worm burdens may lead to severe clinical signs such as anorexia, anaemia, diarrhoea, protein loss, oedema, decreased immunity and fertility, which can lead to fatal outcomes. Therefore, gastrointestinal nematodes hinder sheep farming in different ways leading to high economic losses that are estimated at several hundred million euros in Europe, although it is difficult to quantify it precisely. An additional problem is the development of anthelmintic resistance in nematodes that occur as a result of irrational use of commercial drugs, whereby the main risk factors are overfrequent treatments, underdosing, the use of only one drug without combination or rotation etc. This leads to a decrease in their effectiveness, and consequently to even higher economic losses (an additional 35-40 million euros). Moreover, these costs will tend to increase in the future due to widespread of resistance and the occurrence of multiresistant strains. For these reasons, the focus of nowadays research is finding alternative solutions including genetic selection of naturally resistant animals, pasture management, dietary manipulation, biological control (use of nematophagous fungi, bacteria or earthworms and dung beetles) and the use of plant-based formulations such as extracts and essential oils. All of the above strategies have shown promise, although it appears that none of them could be used independently. Therefore, future strategies for the control of gastrointestinal nematodes should be based on an integrated approach. This implies the combination of mentioned alternatives with rational use of anthelmintics based on refugia strategies (target treatments and target selective treatments).",
publisher = "Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina",
journal = "Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina",
title = "The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2441"
}

Štrbac, F., Krnjajić, S., Stanković, S., Tomić, V., Ratajac, R.,& Stojanović, D.. (2023). The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies. in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina
Faculty of Agriculture, University of East Sarajevo, Republic of Srpska, Bosnia and Herzegovina..
https://hdl.handle.net/21.15107/rcub_rimsi_2441

Štrbac F, Krnjajić S, Stanković S, Tomić V, Ratajac R, Stojanović D. The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies. in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina. 2023;.
https://hdl.handle.net/21.15107/rcub_rimsi_2441 .

Štrbac, Filip, Krnjajić, Slobodan, Stanković, Slađan, Tomić, Vedran, Ratajac, Radomir, Stojanović, Dragica, "The Influence of Gastrointestinal Nematodes on Modern Sheep Farming and Novel Control Strategies" in Book of Abstracts of the XIV International Agriculture Symposium "AGROSYM 2023", Jahorina, Bosnia and Herzegovina (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2441 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Krnjajić, Slobodan
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2137
AB  - Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic
potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”
T1  - Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2137
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Krnjajić, Slobodan and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2023",
abstract = "Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic
potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”",
title = "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2137"
}

Štrbac, F., Simin, N., Orčić, D., Krnjajić, S., Bosco, A., Stojanović, D., Ratajac, R., Cringoli, G.,& Rinaldi, L.. (2023). Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 113-113.
https://hdl.handle.net/21.15107/rcub_rimsi_2137

Štrbac F, Simin N, Orčić D, Krnjajić S, Bosco A, Stojanović D, Ratajac R, Cringoli G, Rinaldi L. Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_rimsi_2137 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Krnjajić, Slobodan, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Cringoli, Giuseppe, Rinaldi, Laura, "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties" in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology” (2023):113-113,
https://hdl.handle.net/21.15107/rcub_rimsi_2137 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Rinaldi, Laura
AU  - Ciccone, Elena
AU  - Maurelli, Maria Paola
AU  - Cringoli, Giuseppe
AU  - Bosco, Antonio
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2105
AB  - Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.
PB  - Frontiers
T2  - Frontiers in Veterinary Science
T1  - In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1232570
VL  - 10
DO  - 10.3389/fvets.2023.1232570
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Rinaldi, Laura and Ciccone, Elena and Maurelli, Maria Paola and Cringoli, Giuseppe and Bosco, Antonio",
year = "2023",
abstract = "Nowadays, the exclusive use of commercial anthelmintics for the treatment of gastrointestinal nematode infections in ruminants is less sustainable due to anthelmintic resistance, as well as the problem of drug residues in animal products and the environment. Therefore, an integrated therapeutic approach is needed, including the search for alternatives to synthetic anthelmintic drugs. The aim of this study was to evaluate the possibility of using the essential oil of peppermint (Mentha x piperita L.) in the control of gastrointestinal nematodes in sheep. For this purpose, the in vitro and in vivo anthelmintic efficacy of this oil and the toxic effects on the hosts were examined. In the in vitro egg hatch test, ovicidal activity varied from 21.0–90.3% depending on the concentration of essential oil used (0.0125, 0.025, 0.049, 0.195, 0.781, 3.125, 12.5, and 50 mg/mL). To some extent, anthelmintic efficacy was confirmed in the in vivo fecal egg count reduction test at a mean dose of 150 mg/kg, with an average reduction of nematode eggs of 26.9 and 46.0% at Days 7 and 14 after treatment, respectively. Furthermore, no toxic effects of applied oil were observed on sheep behavior, kidney, or liver function. The main compounds identified by gas chromatography–mass spectrometry analyzes were menthol (32.6%), menthone (22.0%), menthyl-acetate (10.0%), and isomenthone (9.39%). Due to their complex chemical compositions, numerous bioactive ingredients, and natural origin, herbal formulations represent a potentially valuable alternative for the control of gastrointestinal nematodes in sheep. In this context, the results of the present study showed that peppermint essential oil is one of the promising candidates. Further studies should be performed to collect more data on the safety profile of M. piperita EO in treated animals to find the most appropriate formulation for use in field conditions and to test it against resistant gastrointestinal nematode populations.",
publisher = "Frontiers",
journal = "Frontiers in Veterinary Science",
title = "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1232570",
volume = "10",
doi = "10.3389/fvets.2023.1232570"
}

Štrbac, F., Krnjajić, S., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Rinaldi, L., Ciccone, E., Maurelli, M. P., Cringoli, G.,& Bosco, A.. (2023). In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science
Frontiers., 10(1232570).
https://doi.org/10.3389/fvets.2023.1232570

Štrbac F, Krnjajić S, Stojanović D, Ratajac R, Simin N, Orčić D, Rinaldi L, Ciccone E, Maurelli MP, Cringoli G, Bosco A. In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Frontiers in Veterinary Science. 2023;10(1232570).
doi:10.3389/fvets.2023.1232570 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Rinaldi, Laura, Ciccone, Elena, Maurelli, Maria Paola, Cringoli, Giuseppe, Bosco, Antonio, "In Vitro and In Vivo Anthelmintic Efficacy of Peppermint (Mentha x piperita L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Frontiers in Veterinary Science, 10, no. 1232570 (2023),
https://doi.org/10.3389/fvets.2023.1232570 . .

TY  - CHAP
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Stojanović, Dragica
AU  - Novakov, Nikolina
AU  - Bosco, Antonio
AU  - Simin, Nataša
AU  - Ratajac, Radomir
AU  - Stanković, Slađan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1899
AB  - Phytotherapy may be defined as the use of plants for the treatment of ailments and those represent a practice that dates since ancient times. It refers to the use of whole plants, their parts such as flowers, leaves, roots and seeds as well as substances extracted from them (plant extracts and essential oils) for treating various diseases. It also may imply their use to support traditional treatment with commercial drugs. Plants and their extracts are an important part of pharmacopoeia in less developed parts of the world, but more recently in the advancement societies. However, plant-based products may also be used for the treatment of diseases in animals, prevalently in livestock. Ethnopharmacology may be implied in veterinary medicine due to the potential therapeutic efficacy, reduced susceptibility to microbial and parasitic resistance, as well as lowered risk of adverse effects and decreased residues in animal products and environment in comparison with chemotherapeutic agents. Moreover, botanical control of various diseases in animals can also be sustainable from the financial point of view. Therefore, medicinal plants are a valuable part of the field of drug discovery and represent an important source of new drugs and drug leads. In this regard, antiparasitic properties are a common point of focus in studies aimed to validate the pharmacological effects of herbal products. A huge number of such plants and their products are considered suitable for the treatment of almost every parasitic disease in livestock. In pets, there are also an increasing number of such studies in dogs and cats, whereby plants product were proven to be effective against various parasites.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
T2  - One Health Triad
T1  - Botanical Control of Parasites in Veterinary Medicine
EP  - 222
IS  - 31
SP  - 215
VL  - 3
DO  - 10.47278/book.oht/2023.98
ER  - 

@inbook{
author = "Štrbac, Filip and Krnjajić, Slobodan and Stojanović, Dragica and Novakov, Nikolina and Bosco, Antonio and Simin, Nataša and Ratajac, Radomir and Stanković, Slađan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2023",
abstract = "Phytotherapy may be defined as the use of plants for the treatment of ailments and those represent a practice that dates since ancient times. It refers to the use of whole plants, their parts such as flowers, leaves, roots and seeds as well as substances extracted from them (plant extracts and essential oils) for treating various diseases. It also may imply their use to support traditional treatment with commercial drugs. Plants and their extracts are an important part of pharmacopoeia in less developed parts of the world, but more recently in the advancement societies. However, plant-based products may also be used for the treatment of diseases in animals, prevalently in livestock. Ethnopharmacology may be implied in veterinary medicine due to the potential therapeutic efficacy, reduced susceptibility to microbial and parasitic resistance, as well as lowered risk of adverse effects and decreased residues in animal products and environment in comparison with chemotherapeutic agents. Moreover, botanical control of various diseases in animals can also be sustainable from the financial point of view. Therefore, medicinal plants are a valuable part of the field of drug discovery and represent an important source of new drugs and drug leads. In this regard, antiparasitic properties are a common point of focus in studies aimed to validate the pharmacological effects of herbal products. A huge number of such plants and their products are considered suitable for the treatment of almost every parasitic disease in livestock. In pets, there are also an increasing number of such studies in dogs and cats, whereby plants product were proven to be effective against various parasites.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "One Health Triad",
booktitle = "Botanical Control of Parasites in Veterinary Medicine",
pages = "222-215",
number = "31",
volume = "3",
doi = "10.47278/book.oht/2023.98"
}

Štrbac, F., Krnjajić, S., Stojanović, D., Novakov, N., Bosco, A., Simin, N., Ratajac, R., Stanković, S., Cringoli, G.,& Rinaldi, L.. (2023). Botanical Control of Parasites in Veterinary Medicine. in One Health Triad
Unique Scientific Publishers, Faisalabad, Pakistan., 3(31), 215-222.
https://doi.org/10.47278/book.oht/2023.98

Štrbac F, Krnjajić S, Stojanović D, Novakov N, Bosco A, Simin N, Ratajac R, Stanković S, Cringoli G, Rinaldi L. Botanical Control of Parasites in Veterinary Medicine. in One Health Triad. 2023;3(31):215-222.
doi:10.47278/book.oht/2023.98 .

Štrbac, Filip, Krnjajić, Slobodan, Stojanović, Dragica, Novakov, Nikolina, Bosco, Antonio, Simin, Nataša, Ratajac, Radomir, Stanković, Slađan, Cringoli, Giuseppe, Rinaldi, Laura, "Botanical Control of Parasites in Veterinary Medicine" in One Health Triad, 3, no. 31 (2023):215-222,
https://doi.org/10.47278/book.oht/2023.98 . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1967
AB  - Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary
practice.
Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations
(50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo
Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated.
Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7%
depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT
with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%).
Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep
GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.
PB  - World Federation of Parasitologists
C3  - Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark
T1  - Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1967
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary
practice.
Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations
(50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo
Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated.
Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7%
depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT
with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%).
Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep
GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.",
publisher = "World Federation of Parasitologists",
journal = "Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark",
title = "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1967"
}

Štrbac, F., Bosco, A., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark
World Federation of Parasitologists..
https://hdl.handle.net/21.15107/rcub_rimsi_1967

Štrbac F, Bosco A, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Cringoli G, Rinaldi L. Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_1967 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study" in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1967 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1968
AB  - The development of anthelmintic resistance in gastrointestinal nematodes (GINs) requires the search for alternative strategies. The aim of this study was to examine in vitro and in vivo anthelmintic efficacy of essential oil (EO) of Satureja montana (L.) against GIN of sheep, as well as toxic effects in sheep, in order to evaluate the possibility of its use in the veterinary practice. In vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of EO. In addition, for the in vivo Faecal Egg Count Reduction Test (FECRT), S. montana EO was administered orally to sheep of two different farms
in southern Italy at the mean dose of 150 mg/kg. The chemical composition of EO was determined by GC-MS. In the EHT, S. montana EO showed ovicidal potential against sheep GINs with inhibition of egg hatchability that varied from 17.3-83.0% depending on the used concentration. The EO also showed in vivo anthelmintic potential with the total reduction of FEC of 15.7% and 33.0% at days 7 and 14 after treatment, respectively. No toxic effects were observed during clinical evaluation, blood count and liver function tests of sheep. The main represented compounds of EO were p-cymene (42.8%), carvacrol (28.1%) and γ-terpinene (14.6%). The obtained results suggest that S. montana EO may be used in the sustainable future management of GIN infections in sheep as a complementary and sustainable method to reduce the use of chemicals and to counteract anthelmintic resistance.
PB  - COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230
C3  - Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece
T1  - In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1968
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The development of anthelmintic resistance in gastrointestinal nematodes (GINs) requires the search for alternative strategies. The aim of this study was to examine in vitro and in vivo anthelmintic efficacy of essential oil (EO) of Satureja montana (L.) against GIN of sheep, as well as toxic effects in sheep, in order to evaluate the possibility of its use in the veterinary practice. In vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of EO. In addition, for the in vivo Faecal Egg Count Reduction Test (FECRT), S. montana EO was administered orally to sheep of two different farms
in southern Italy at the mean dose of 150 mg/kg. The chemical composition of EO was determined by GC-MS. In the EHT, S. montana EO showed ovicidal potential against sheep GINs with inhibition of egg hatchability that varied from 17.3-83.0% depending on the used concentration. The EO also showed in vivo anthelmintic potential with the total reduction of FEC of 15.7% and 33.0% at days 7 and 14 after treatment, respectively. No toxic effects were observed during clinical evaluation, blood count and liver function tests of sheep. The main represented compounds of EO were p-cymene (42.8%), carvacrol (28.1%) and γ-terpinene (14.6%). The obtained results suggest that S. montana EO may be used in the sustainable future management of GIN infections in sheep as a complementary and sustainable method to reduce the use of chemicals and to counteract anthelmintic resistance.",
publisher = "COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230",
journal = "Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece",
title = "In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1968"
}

Štrbac, F., Bosco, A., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep. in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece
COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230..
https://hdl.handle.net/21.15107/rcub_rimsi_1968

Štrbac F, Bosco A, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Cringoli G, Rinaldi L. In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep. in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece. 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_1968 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro and In Vivo Anthelmintic Efficacy of Essential Oil of Satureja montana (L.) against Gastrointestinal Nematodes of Sheep" in Book of Abstracts of COMBAR Final Conference: “Combatting anthelmintic resistance in ruminants: options for the future”, Athens, Greece (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1968 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1970
AB  - The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
C3  - Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
T1  - In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1970
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan",
title = "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1970"
}

Štrbac, F., Bosco, A., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
Unique Scientific Publishers, Faisalabad, Pakistan., 27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970

Štrbac F, Bosco A, Stojanović D, Ratajac R, Simin N, Orčić D, Petrović K, Cringoli G, Rinaldi L. In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .

Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes" in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan (2022):27-27,
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Petrović, Kosta
AU  - Ratajac, Radomir
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1976
AB  - The development and spread of anthelmintic resistance to commercial drugs represent a major problem in veterinary medicine, which justifies the search for alternatives. Within that context, sage (Salvia officinalis L.) is a well-known medicinal plant that has been cultivated for centuries due to its healing properties. The aim of this study was to determine the chemical composition of the sage essential oil and to evaluate its anthelmintic properties against sheep gastrointestinal nematodes. Chemical characterization was done by GC-MS analyses, and the anthelmintic potential of tested
samples was evaluated by in vitro egg hatch test in a concentration range of 0.0125-50 mg/mL. The most represented identified compounds were α-thujone (38.76%), camphor (19.75%) and 1,8-cineole (8.40%), camphene (5.36%) and α-humulene (4.15%) out of a total of 27 identified compounds. The ovicidal activity of sage essential oil, reflected in the inhibition of egg hatchability of the eggs of sheep gastrointestinal nematodes, varied from 15.0-89.0% depending on the used concentration, with a calculated IC50 value of 0.53 mg/mL. The obtained results indicate that the compounds from sage essential oil posess high anthelmintic properties and should be tested in further in vivo studies. Sage essential oil could find application in veterinary medicine in the context of the development of new natural anthelmintic agents for the control of sheep gastrointestinal nematodes, which could have high significance from economic point of view.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
T1  - Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1976
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Bosco, Antonio and Stojanović, Dragica and Petrović, Kosta and Ratajac, Radomir and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The development and spread of anthelmintic resistance to commercial drugs represent a major problem in veterinary medicine, which justifies the search for alternatives. Within that context, sage (Salvia officinalis L.) is a well-known medicinal plant that has been cultivated for centuries due to its healing properties. The aim of this study was to determine the chemical composition of the sage essential oil and to evaluate its anthelmintic properties against sheep gastrointestinal nematodes. Chemical characterization was done by GC-MS analyses, and the anthelmintic potential of tested
samples was evaluated by in vitro egg hatch test in a concentration range of 0.0125-50 mg/mL. The most represented identified compounds were α-thujone (38.76%), camphor (19.75%) and 1,8-cineole (8.40%), camphene (5.36%) and α-humulene (4.15%) out of a total of 27 identified compounds. The ovicidal activity of sage essential oil, reflected in the inhibition of egg hatchability of the eggs of sheep gastrointestinal nematodes, varied from 15.0-89.0% depending on the used concentration, with a calculated IC50 value of 0.53 mg/mL. The obtained results indicate that the compounds from sage essential oil posess high anthelmintic properties and should be tested in further in vivo studies. Sage essential oil could find application in veterinary medicine in the context of the development of new natural anthelmintic agents for the control of sheep gastrointestinal nematodes, which could have high significance from economic point of view.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"",
title = "Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1976"
}

Štrbac, F., Simin, N., Orčić, D., Bosco, A., Stojanović, D., Petrović, K., Ratajac, R., Cringoli, G.,& Rinaldi, L.. (2022). Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry"
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 143-143.
https://hdl.handle.net/21.15107/rcub_rimsi_1976

Štrbac F, Simin N, Orčić D, Bosco A, Stojanović D, Petrović K, Ratajac R, Cringoli G, Rinaldi L. Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes. in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry". 2022;:143-143.
https://hdl.handle.net/21.15107/rcub_rimsi_1976 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Bosco, Antonio, Stojanović, Dragica, Petrović, Kosta, Ratajac, Radomir, Cringoli, Giuseppe, Rinaldi, Laura, "Chemical Composition of Sage (Salvia officinalis L.) Essential Oil and its Anthelmintic Properties against Sheep Gastrointestinal Nematodes" in Serbian Biochemical Society Eleventh Conference (scientific meeting of an international character) - "Amazing Biochemistry" (2022):143-143,
https://hdl.handle.net/21.15107/rcub_rimsi_1976 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1925
AB  - Ekonomski gubici koje gastrointestinalne nematode nanose modernom ovčarstvu postaju sve veći usled razvoja rezistencije na sintetske antihelmintike koji se koriste u svakodnevnoj kliničkoj praksi. Zbog toga, istraživači širom sveta, danas tragaju za alternativnim metodama u cilju kontrole ovih parazita. Kao moguća opcija su se zbog svojih osobina, nametnula etarska ulja i biljni proizvodi bogati različitim prirodno aktivnim jedinjenjima. Naime, prema dosadašnjim ispitivanjima, etarska ulja ispoljavaju visoku aktivnost protiv različitih razvojnih stadijuma nematoda, a uz pravilan način primene se generalno dobro tolerišu od strane sisara sa toksikološke strane gledišta. Do sada je dokazana visoka efikasnost velikog broja etarskih ulja protiv gastrointestinalnih nematoda ovaca (različite vrste eukaliptusa, limunske trave, mente, čajevca, timijana, cimeta, đumbira, lavande, crnog pelena i ruzmarina), njihovih aktivnih sastojaka (timol, karvakrol, karvon, anetol, mentol, cinamaldehid, cineol, linalol, vanilin, eugenol, limonen) kao i njihovih kombinacija (npr. cinamaldehid:karvakrol; anetol:karvon) u laboratorijskim uslovima. Međutim, glavno ograničenje za njihovu svakodnevnu primenu predstavlja još uvek nedovoljna efikasnost u terenskim uslovima, a koja potiče od anatomsko-fizioloških specifičnosti vezanih za preživare, kao i od farmakokinetičkih osobina pojedinih etarskih ulja. Ovaj problem bi se mogao prevazići odabirom odgovarajućih etarskih ulja uz adekvatno doziranje, način primene i formulaciju samih ulja, kombinovanom sinergističkom primenom više ulja ili njihovih sastojaka, kao i primenom nanotehnologije. Nevezano za ovaj problem, etarska ulja svakako imaju potencijal da uz kombinaciju sa drugim lekovima i merama, budu deo integrisanog pristupa, dizajniranog da se postigne održiva kontrola parazita u sistemima proizvodnje preživara. Potrebna su dodatna ispitivanja u ovoj oblasti, što se posebno odnosi na istraživanje efikasnosti u terenskim uslovima.
AB  - The economic losses caused by gastrointestinal nematodes to modern sheep farming are increasing due to the development of resistance to regular anthelmintics used in everyday clinical practice. Therefore, researchers around the world nowadays are looking for alternative methods to control these parasites. Essential oils, herbal products rich in various naturally active compounds, have become a viable option because of their properties. Namely, according to the previous research, essential oils show high activity against all nematode development stages and are generally well tolerated by mammals from the toxicological point of view. So far, high efficacy of a large number of essential oils against sheep gastrointestinal nematodes have been demonstrated (different species of eucalyptus, lemongrass, mint, tea tree, thyme, cinnamon, ginger, mugwort, rosemary etc), their active compounds (thymol, carvacrol, carvone, anethole, menthol, cinnamaldehyde, linalool, vanillin, eugenol, cineole, limonene) as well as their combinations (e.g. cinnamaldehyde:carvacrol; anethole:carvone). However, the main limitation for their everyday use is still insufficient efficacy in field conditions, which originates from the anatomical and physiological specificities associated with ruminants, as well as from the pharmacokinetic properties of certain essential oils. This problem could be overcomed by selecting the appropriate essential oils with adequate dosage, method of application and formulations of oils, by combined synergistic application of multiple oils or their ingredients, as well as the using of nanotechnology. Regardless of this problem, essential oils certainly have the potential to be, in combination with other drugs and measures, part of an integrated approach designed to achieve sustainable parasite control in ruminant production systems. Nevertheless, further examinations on this topic are needed, especially of testing efficacy in field conditions.
PB  - Udruženje veterinara praktičara Srbije (UVPS)
C3  - Zbornik predavanja - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda (IV simpozijum sa međunarodnim učešćem)
T1  - Potencijalna primena etarskih ulja protiv gastrointestinalnih nematoda ovaca - perspektiva i barijere
T1  - Potential Use of Essential Oils against Ovine Gastrointestinal Nematodes - Perspectives and Barriers
EP  - 267
SP  - 257
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1925
ER  - 

@conference{
author = "Štrbac, Filip and Stojanović, Dragica and Ratajac, Radomir",
year = "2022",
abstract = "Ekonomski gubici koje gastrointestinalne nematode nanose modernom ovčarstvu postaju sve veći usled razvoja rezistencije na sintetske antihelmintike koji se koriste u svakodnevnoj kliničkoj praksi. Zbog toga, istraživači širom sveta, danas tragaju za alternativnim metodama u cilju kontrole ovih parazita. Kao moguća opcija su se zbog svojih osobina, nametnula etarska ulja i biljni proizvodi bogati različitim prirodno aktivnim jedinjenjima. Naime, prema dosadašnjim ispitivanjima, etarska ulja ispoljavaju visoku aktivnost protiv različitih razvojnih stadijuma nematoda, a uz pravilan način primene se generalno dobro tolerišu od strane sisara sa toksikološke strane gledišta. Do sada je dokazana visoka efikasnost velikog broja etarskih ulja protiv gastrointestinalnih nematoda ovaca (različite vrste eukaliptusa, limunske trave, mente, čajevca, timijana, cimeta, đumbira, lavande, crnog pelena i ruzmarina), njihovih aktivnih sastojaka (timol, karvakrol, karvon, anetol, mentol, cinamaldehid, cineol, linalol, vanilin, eugenol, limonen) kao i njihovih kombinacija (npr. cinamaldehid:karvakrol; anetol:karvon) u laboratorijskim uslovima. Međutim, glavno ograničenje za njihovu svakodnevnu primenu predstavlja još uvek nedovoljna efikasnost u terenskim uslovima, a koja potiče od anatomsko-fizioloških specifičnosti vezanih za preživare, kao i od farmakokinetičkih osobina pojedinih etarskih ulja. Ovaj problem bi se mogao prevazići odabirom odgovarajućih etarskih ulja uz adekvatno doziranje, način primene i formulaciju samih ulja, kombinovanom sinergističkom primenom više ulja ili njihovih sastojaka, kao i primenom nanotehnologije. Nevezano za ovaj problem, etarska ulja svakako imaju potencijal da uz kombinaciju sa drugim lekovima i merama, budu deo integrisanog pristupa, dizajniranog da se postigne održiva kontrola parazita u sistemima proizvodnje preživara. Potrebna su dodatna ispitivanja u ovoj oblasti, što se posebno odnosi na istraživanje efikasnosti u terenskim uslovima., The economic losses caused by gastrointestinal nematodes to modern sheep farming are increasing due to the development of resistance to regular anthelmintics used in everyday clinical practice. Therefore, researchers around the world nowadays are looking for alternative methods to control these parasites. Essential oils, herbal products rich in various naturally active compounds, have become a viable option because of their properties. Namely, according to the previous research, essential oils show high activity against all nematode development stages and are generally well tolerated by mammals from the toxicological point of view. So far, high efficacy of a large number of essential oils against sheep gastrointestinal nematodes have been demonstrated (different species of eucalyptus, lemongrass, mint, tea tree, thyme, cinnamon, ginger, mugwort, rosemary etc), their active compounds (thymol, carvacrol, carvone, anethole, menthol, cinnamaldehyde, linalool, vanillin, eugenol, cineole, limonene) as well as their combinations (e.g. cinnamaldehyde:carvacrol; anethole:carvone). However, the main limitation for their everyday use is still insufficient efficacy in field conditions, which originates from the anatomical and physiological specificities associated with ruminants, as well as from the pharmacokinetic properties of certain essential oils. This problem could be overcomed by selecting the appropriate essential oils with adequate dosage, method of application and formulations of oils, by combined synergistic application of multiple oils or their ingredients, as well as the using of nanotechnology. Regardless of this problem, essential oils certainly have the potential to be, in combination with other drugs and measures, part of an integrated approach designed to achieve sustainable parasite control in ruminant production systems. Nevertheless, further examinations on this topic are needed, especially of testing efficacy in field conditions.",
publisher = "Udruženje veterinara praktičara Srbije (UVPS)",
journal = "Zbornik predavanja - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda (IV simpozijum sa međunarodnim učešćem)",
title = "Potencijalna primena etarskih ulja protiv gastrointestinalnih nematoda ovaca - perspektiva i barijere, Potential Use of Essential Oils against Ovine Gastrointestinal Nematodes - Perspectives and Barriers",
pages = "267-257",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1925"
}

Štrbac, F., Stojanović, D.,& Ratajac, R.. (2022). Potencijalna primena etarskih ulja protiv gastrointestinalnih nematoda ovaca - perspektiva i barijere. in Zbornik predavanja - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda (IV simpozijum sa međunarodnim učešćem)
Udruženje veterinara praktičara Srbije (UVPS)., 257-267.
https://hdl.handle.net/21.15107/rcub_rimsi_1925

Štrbac F, Stojanović D, Ratajac R. Potencijalna primena etarskih ulja protiv gastrointestinalnih nematoda ovaca - perspektiva i barijere. in Zbornik predavanja - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda (IV simpozijum sa međunarodnim učešćem). 2022;:257-267.
https://hdl.handle.net/21.15107/rcub_rimsi_1925 .

Štrbac, Filip, Stojanović, Dragica, Ratajac, Radomir, "Potencijalna primena etarskih ulja protiv gastrointestinalnih nematoda ovaca - perspektiva i barijere" in Zbornik predavanja - Zdravstvena zaštita i reprodukcija papkara, kopitara, živine i mesojeda (IV simpozijum sa međunarodnim učešćem) (2022):257-267,
https://hdl.handle.net/21.15107/rcub_rimsi_1925 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1924
AB  - Upotreba biljnih preparata kao što su etarska ulja, za različite indikacije u veterinarskoj medicini predstavlja veoma aktuelno i perspektivno polje. U tom kontekstu, najčešće se ispituju i dokazuju antibakterijsko, antifungalno i antiparazitsko, ali i mnoga druga lekovita dejstva ovih preparata. Zbog toga se etarska ulja sve više koriste za različite namene, kao što su prevencija dijareje prasadi ili tretiranje kokcidioze živine. Međutim, usled njihove nestabilne prirode i sklonosti ka isparavanju može doći do degradacije i delimične inaktivacije njihovih aktivnih sastojaka u organizmu, što dovodi do ograničene efikasnosti etarskih ulja nakon primene in vivo. Potencijalno rešenje za ovaj problem, mnogi istraživači vide u inovativnim tehnikama inkapsulacije. One predstavljaju postupak zaštite aktivnih komponenti, fizičkim ili hemijskim procesima, pri čemu se formira zaštitni omotač. Zbog toga su ove tehnike emulzifikacije i nanotehnologije našle primenu u različitim granama (industrija hrane, farmacija), a mogle bi biti veoma korisne i kada je u pitanju primena etarskih ulja. Naime, inkapsulacijom se smanjuje interakcija aktivnih supstanci sa različitim faktorima kao i brzina isparavanja, čime bi se mogla umanjiti inaktivacija sastojaka etarskih ulja, povećati bioraspoloživost, a samim tim i efikasnost. Ovo je posebno značajno za primenu protiv bakterija i endoparazita u gastrointestinalnom traktu. Primenom ovih tehnika se omogućava i kontrolisano oslobađanje aktivnih supstanci, što takođe može biti značajno prilikom pojedinih vidova primene kao što je antiektoparazitska. Na kraju, inkapsulacijom se povećava jednostavnost rukovanja aktivnim supstancama, ali i umanjuje neprijatan miris i ukus, što je veoma bitno prilikom primene etarskih ulja u hrani za životinje. Iz svega navedenog se može zaključiti da tehnike inkapsulacije imaju višestruki značaj kada je u pitanju primena etarskih ulja u veterinarskoj praksi, omogućavajući jednostavniju, praktičniju i efikasniju a samim tim i širu primenu ovih prirodnih proizvoda.
AB  - The use of herbal preparations such as essential oils for various indications in veterinary medicine presents a modern and promising field. Within that context, antibacterial, antifungal and antiparasitic, as well as many other medicinal effects of these substances are most often examined. For that reason, essential oils are increasingly used for different purposes, such as prevention of diarrhoea in piglets or coccidiosis treatment in poultry. However, their unstable and volatile nature may lead to degradation and partial deactivation of their active ingredients in the organism after some in vivo applications. As a potential solution for this problem, many researchers consider innovative encapsulation techniques. They represent the process of protecting the active components by physical or chemical processes, whereby a protective coating is formed. Therefore, these techniques such as emulsification or nanotechnology have found application in various branches (food industry, pharmacy) and could be very useful for the application of essential oils. Namely, encapsulation reduces the interaction of active substances with various factors as well as the rate of evaporation, which could reduce the deactivation of essential oil ingredients, increase bioavailability and thus efficiency (significant for use against the bacteria and endoparasites in the intestinal tract). The application of these techniques also enables the controlled release of active substances, which can also be important for some applications such as antiectoparasitic. Finally, encapsulation increases the ease of application and also reduces unpleasant smells and tastes, which is very important for the use of essential oils in animal feed. From all the above, it can be concluded that encapsulation techniques have multiple implications for the application of essential oils in veterinary practice with enabling simpler, more practical, efficient, and thus wider application of these natural products.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)
C3  - Zbornik radova Zdravstvena zaštita i reprodukcija životinja
T1  - Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini
T1  - Encapsulation and its Significance for Application of Essential Oils in Veterinary Medicine
EP  - 46
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1924
ER  - 

@conference{
author = "Štrbac, Filip and Stojanović, Dragica and Ratajac, Radomir and Petrović, Kosta",
year = "2022",
abstract = "Upotreba biljnih preparata kao što su etarska ulja, za različite indikacije u veterinarskoj medicini predstavlja veoma aktuelno i perspektivno polje. U tom kontekstu, najčešće se ispituju i dokazuju antibakterijsko, antifungalno i antiparazitsko, ali i mnoga druga lekovita dejstva ovih preparata. Zbog toga se etarska ulja sve više koriste za različite namene, kao što su prevencija dijareje prasadi ili tretiranje kokcidioze živine. Međutim, usled njihove nestabilne prirode i sklonosti ka isparavanju može doći do degradacije i delimične inaktivacije njihovih aktivnih sastojaka u organizmu, što dovodi do ograničene efikasnosti etarskih ulja nakon primene in vivo. Potencijalno rešenje za ovaj problem, mnogi istraživači vide u inovativnim tehnikama inkapsulacije. One predstavljaju postupak zaštite aktivnih komponenti, fizičkim ili hemijskim procesima, pri čemu se formira zaštitni omotač. Zbog toga su ove tehnike emulzifikacije i nanotehnologije našle primenu u različitim granama (industrija hrane, farmacija), a mogle bi biti veoma korisne i kada je u pitanju primena etarskih ulja. Naime, inkapsulacijom se smanjuje interakcija aktivnih supstanci sa različitim faktorima kao i brzina isparavanja, čime bi se mogla umanjiti inaktivacija sastojaka etarskih ulja, povećati bioraspoloživost, a samim tim i efikasnost. Ovo je posebno značajno za primenu protiv bakterija i endoparazita u gastrointestinalnom traktu. Primenom ovih tehnika se omogućava i kontrolisano oslobađanje aktivnih supstanci, što takođe može biti značajno prilikom pojedinih vidova primene kao što je antiektoparazitska. Na kraju, inkapsulacijom se povećava jednostavnost rukovanja aktivnim supstancama, ali i umanjuje neprijatan miris i ukus, što je veoma bitno prilikom primene etarskih ulja u hrani za životinje. Iz svega navedenog se može zaključiti da tehnike inkapsulacije imaju višestruki značaj kada je u pitanju primena etarskih ulja u veterinarskoj praksi, omogućavajući jednostavniju, praktičniju i efikasniju a samim tim i širu primenu ovih prirodnih proizvoda., The use of herbal preparations such as essential oils for various indications in veterinary medicine presents a modern and promising field. Within that context, antibacterial, antifungal and antiparasitic, as well as many other medicinal effects of these substances are most often examined. For that reason, essential oils are increasingly used for different purposes, such as prevention of diarrhoea in piglets or coccidiosis treatment in poultry. However, their unstable and volatile nature may lead to degradation and partial deactivation of their active ingredients in the organism after some in vivo applications. As a potential solution for this problem, many researchers consider innovative encapsulation techniques. They represent the process of protecting the active components by physical or chemical processes, whereby a protective coating is formed. Therefore, these techniques such as emulsification or nanotechnology have found application in various branches (food industry, pharmacy) and could be very useful for the application of essential oils. Namely, encapsulation reduces the interaction of active substances with various factors as well as the rate of evaporation, which could reduce the deactivation of essential oil ingredients, increase bioavailability and thus efficiency (significant for use against the bacteria and endoparasites in the intestinal tract). The application of these techniques also enables the controlled release of active substances, which can also be important for some applications such as antiectoparasitic. Finally, encapsulation increases the ease of application and also reduces unpleasant smells and tastes, which is very important for the use of essential oils in animal feed. From all the above, it can be concluded that encapsulation techniques have multiple implications for the application of essential oils in veterinary practice with enabling simpler, more practical, efficient, and thus wider application of these natural products.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)",
journal = "Zbornik radova Zdravstvena zaštita i reprodukcija životinja",
title = "Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini, Encapsulation and its Significance for Application of Essential Oils in Veterinary Medicine",
pages = "46-37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1924"
}

Štrbac, F., Stojanović, D., Ratajac, R.,& Petrović, K.. (2022). Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja
Poljoprivredni fakultet, Univerzitet u Novom Sadu; Udruženje veterinara praktičara Srbije (UVPS)., 37-46.
https://hdl.handle.net/21.15107/rcub_rimsi_1924

Štrbac F, Stojanović D, Ratajac R, Petrović K. Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini. in Zbornik radova Zdravstvena zaštita i reprodukcija životinja. 2022;:37-46.
https://hdl.handle.net/21.15107/rcub_rimsi_1924 .

Štrbac, Filip, Stojanović, Dragica, Ratajac, Radomir, Petrović, Kosta, "Inkapsulacija i njen značaj za primenu etarskih ulja u veterinarskoj medicini" in Zbornik radova Zdravstvena zaštita i reprodukcija životinja (2022):37-46,
https://hdl.handle.net/21.15107/rcub_rimsi_1924 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1908
AB  - Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio odrediti hemijski sastav etarskog ulja korijandera (Coriander sativum L.) i ispitati njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden pri osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka od čega je 15 identifikovano, iz različitih hemijskih grupa. Dominantan sastojak je bio linalool (84,53%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti. U svakom slučaju, i ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije.
AB  - The development of resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to huge monetary losses in many parts of the world. Therefore, new sustainable strategies for the control of these parasites are being actively sought, among which are included various alternative options such as plant essential oils. The aim of this study was to determine the chemical composition of the coriander (Coriander sativum L.) essential oil and to evaluate its anthelmintic potential against sheep gastrointestinal nematodes. Qualitative and semi-quantitative chemical characterization of the essential oils was performed by gas chromatography-mass spectrometry (GC-MS) analyses. The anthelmintic potential of the essential oil was evaluated using the in vitro egg hatch test (EHT), which was performed at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole at concentrations of 0.025 and 0.0125 mg/mL was used as a positive control, and emulsifier 3% Tween 80 v/v and distilled water as negative controls. GC-MS analysis revealed the rich chemical composition of the tested oil, where a total of 17 different ingredients were found of which 15 were identified, from different chemical groups. The dominant ingredient was linalool (84.53%) with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). EHT results showed a high anthelmintic potential of the coriander oil with an inhibition of egg hatchability i.e. ovicidal effect of 29.0-88.7% depending on the used concentration, with the obtained IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). The obtained results indicate the possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes in sheep, after further in vivo studies and toxicity studies. In any case, this study is another confirmation of the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and in combating anthelmintic resistance.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav
T1  - Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) and its Chemical Composition
EP  - 341
SP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1908
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio odrediti hemijski sastav etarskog ulja korijandera (Coriander sativum L.) i ispitati njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden pri osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka od čega je 15 identifikovano, iz različitih hemijskih grupa. Dominantan sastojak je bio linalool (84,53%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti. U svakom slučaju, i ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije., The development of resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to huge monetary losses in many parts of the world. Therefore, new sustainable strategies for the control of these parasites are being actively sought, among which are included various alternative options such as plant essential oils. The aim of this study was to determine the chemical composition of the coriander (Coriander sativum L.) essential oil and to evaluate its anthelmintic potential against sheep gastrointestinal nematodes. Qualitative and semi-quantitative chemical characterization of the essential oils was performed by gas chromatography-mass spectrometry (GC-MS) analyses. The anthelmintic potential of the essential oil was evaluated using the in vitro egg hatch test (EHT), which was performed at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole at concentrations of 0.025 and 0.0125 mg/mL was used as a positive control, and emulsifier 3% Tween 80 v/v and distilled water as negative controls. GC-MS analysis revealed the rich chemical composition of the tested oil, where a total of 17 different ingredients were found of which 15 were identified, from different chemical groups. The dominant ingredient was linalool (84.53%) with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). EHT results showed a high anthelmintic potential of the coriander oil with an inhibition of egg hatchability i.e. ovicidal effect of 29.0-88.7% depending on the used concentration, with the obtained IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). The obtained results indicate the possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes in sheep, after further in vivo studies and toxicity studies. In any case, this study is another confirmation of the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and in combating anthelmintic resistance.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav, Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) and its Chemical Composition",
pages = "341-338",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1908"
}

Štrbac, F., Bosco, A., Petrović, K., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Cringoli, G.,& Rinaldi, L.. (2022). Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav. in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 338-341.
https://hdl.handle.net/21.15107/rcub_rimsi_1908

Štrbac F, Bosco A, Petrović K, Stojanović D, Ratajac R, Simin N, Orčić D, Cringoli G, Rinaldi L. Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav. in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2022;:338-341.
https://hdl.handle.net/21.15107/rcub_rimsi_1908 .

Štrbac, Filip, Bosco, Antonio, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Cringoli, Giuseppe, Rinaldi, Laura, "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) i njegov hemijski sastav" in Зборник радова и кратких садржаја 27. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2022):338-341,
https://hdl.handle.net/21.15107/rcub_rimsi_1908 .

TY  - CHAP
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Pušić, Ivan
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Ratajac, Radomir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1900
AB  - Gastrointestinal nematodes (GINs) nowadays present a serious threat to sustainable sheep breeding. Various classes of commercial anthelmintics were used to control these parasites. However, due to the development of anthelmintic resistance (AR), the efficacy of these drugs has decreased, which in turn resulted in high economic losses. For these reasons, researchers are focused now on designing sustainable strategies for GIN control, based on the use of a combination of a bunch of options including the wise application of anthelmintic drugs, as well as applying alternative strategies. Within this context, phytotherapy (the use of plants or their products) presents one of the most promising alternatives. Essential oils (EOs) are natural, volatile and complex compounds characterized by a strong odor and extracted from aromatic plants. In various studies so far, these plant products showed high in vitro and, in some cases, in vivo efficacy against sheep GINs. The aim of this chapter is to review so far conducted studies based on the use of EOs against these parasites and to discuss results, as well as advantages of their use compared to commercial anthelmintics. On the other hand, current obstacles in the use of EOs and possible solutions on how to overcome them will be also discussed in this chapter. In this way, current and future perspectives of the use of EOs against sheep GINs are discussed here.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
T2  - Animal Health Perspectives
T1  - The Use of Essential Oils against Sheep Gastrointestinal Nematodes
EP  - 94
IS  - 12
SP  - 86
VL  - 1
DO  - 10.47278/book.ahp/2022.12
ER  - 

@inbook{
author = "Štrbac, Filip and Bosco, Antonio and Pušić, Ivan and Stojanović, Dragica and Simin, Nataša and Cringoli, Giuseppe and Rinaldi, Laura and Ratajac, Radomir",
year = "2022",
abstract = "Gastrointestinal nematodes (GINs) nowadays present a serious threat to sustainable sheep breeding. Various classes of commercial anthelmintics were used to control these parasites. However, due to the development of anthelmintic resistance (AR), the efficacy of these drugs has decreased, which in turn resulted in high economic losses. For these reasons, researchers are focused now on designing sustainable strategies for GIN control, based on the use of a combination of a bunch of options including the wise application of anthelmintic drugs, as well as applying alternative strategies. Within this context, phytotherapy (the use of plants or their products) presents one of the most promising alternatives. Essential oils (EOs) are natural, volatile and complex compounds characterized by a strong odor and extracted from aromatic plants. In various studies so far, these plant products showed high in vitro and, in some cases, in vivo efficacy against sheep GINs. The aim of this chapter is to review so far conducted studies based on the use of EOs against these parasites and to discuss results, as well as advantages of their use compared to commercial anthelmintics. On the other hand, current obstacles in the use of EOs and possible solutions on how to overcome them will be also discussed in this chapter. In this way, current and future perspectives of the use of EOs against sheep GINs are discussed here.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "Animal Health Perspectives",
booktitle = "The Use of Essential Oils against Sheep Gastrointestinal Nematodes",
pages = "94-86",
number = "12",
volume = "1",
doi = "10.47278/book.ahp/2022.12"
}

Štrbac, F., Bosco, A., Pušić, I., Stojanović, D., Simin, N., Cringoli, G., Rinaldi, L.,& Ratajac, R.. (2022). The Use of Essential Oils against Sheep Gastrointestinal Nematodes. in Animal Health Perspectives
Unique Scientific Publishers, Faisalabad, Pakistan., 1(12), 86-94.
https://doi.org/10.47278/book.ahp/2022.12

Štrbac F, Bosco A, Pušić I, Stojanović D, Simin N, Cringoli G, Rinaldi L, Ratajac R. The Use of Essential Oils against Sheep Gastrointestinal Nematodes. in Animal Health Perspectives. 2022;1(12):86-94.
doi:10.47278/book.ahp/2022.12 .

Štrbac, Filip, Bosco, Antonio, Pušić, Ivan, Stojanović, Dragica, Simin, Nataša, Cringoli, Giuseppe, Rinaldi, Laura, Ratajac, Radomir, "The Use of Essential Oils against Sheep Gastrointestinal Nematodes" in Animal Health Perspectives, 1, no. 12 (2022):86-94,
https://doi.org/10.47278/book.ahp/2022.12 . .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1894
AB  - Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio da se odredi hemijski sastav etarskog ulja korijandera (Coriandrum sativum L.) i da se ispita njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden sa osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka iz različitih hemijskih grupa, od čega je 15 identifikovano. Dominantan sastojak je bio linalool (84,5%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije. Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti.
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to enormous economic losses in many parts of the world. For this reason, new sustainable strategies for the control of these parasites are being actively searched, which include the use of different alternatives such as plant essential oils. The aim of this study was to determine the chemical composition of the essential oil of coriander (Coriandrum sativum L.) and to test its anthelmintic potential against gastrointestinal nematodes of sheep. Qualitative and semi-quantitative chemical characterization of the essential oil was performed using gas chromatography coupled with mass spectrometric detection (GC-MS analysis). The anthelmintic potential of the essential oil was tested using the in vitro egg hatch test (EHT), which was carried out at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole in concentrations of 0.025 and 0.0125 mg/mL was used as the positive control, and the emulsifier 3% Tween 80 v/v and distilled water was used as the negative control. GC-MS analyzes revealed a rich chemical composition of the tested oil, where a total of 17 different ingredients from different chemical groups were found, of which 15 were identified. The dominant ingredient was linalool (84.5%) along with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). The EHT results showed a high anthelmintic potential of coriander oil with inhibition of egg hatching, i.e. an ovicidal effect of 29.0-88.7% depending on the concentration, with an IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). This study confirmed the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and the combatting anthelmintic resistance. The obtained results indicate a possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes of sheep, after further in vivo tests and toxicity studies.
PB  - JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka
T2  - Veterinarski Žurnal Republike Srpske
T1  - Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca
T1  - Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) in Sheep
EP  - 126
IS  - 1-2
SP  - 101
VL  - XXII
DO  - 10.7251/VETJSR2201101S
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Razvoj antihelmintičke rezistencije kod gastrointestinalnih nematoda ovaca na komercijalne antihelmintike je doveo do ogromnih ekonomskih gubitaka u mnogim delovima sveta. Zbog toga se aktivno traga za novim održivim strategijama za kontrolu ovih parazita, koje uključuju i primenu različitih alternativa poput biljnih etarskih ulja. Cilj ovog istraživanja je bio da se odredi hemijski sastav etarskog ulja korijandera (Coriandrum sativum L.) i da se ispita njegov antihelmintički potencijal protiv gastrointestinalnih nematoda ovaca. Kvalitativna i semikvantitativna hemijska karakterizacija etarskog ulja izvršena je primenom gasne hromatografije kuplovane sa masenospektrometrijskom detekcijom (GC-MS analize). Antihelmintički potencijal etarskog ulja je ispitan pomoću in vitro testa izleganja larvi (EHT), koji je sproveden sa osam različitih koncentracija ispitanog ulja (50; 12,5; 3,125; 0,781; 0,195; 0,049; 0,025 i 0,0125 mg/mL). Pri tome je za pozitivnu kontrolu korišćen tiabendazol u koncentracijama 0,025 i 0,0125 mg/mL, a za negativnu emulgator 3% Tween 80 v/v i destilovana voda. GC-MS analizama je otkriven bogat hemijski sastav ispitanog ulja, pri čemu je pronađeno ukupno 17 različitih sastojaka iz različitih hemijskih grupa, od čega je 15 identifikovano. Dominantan sastojak je bio linalool (84,5%) uz γ-terpinen (5,04%), kamfor (3,26%) i α-pinen (2,88%). Rezultati EHT su pokazali visok antihelmintički potencijal ulja korijandera sa inhibicijom izleganja larvi odnosno ovicidnim efektom od 29,0-88,7% u zavisnosti od koncentracije, uz dobijenu IC50 koncentraciju od 0,04 mg/mL i jasan dozno-zavisni efekat (R2=0,90). Ova studija je potvrdila mogući značaj botaničkih antihelmintika u održivoj kontroli nematoda kod životinja i borbi protiv antihelmintičke rezistencije. Dobijeni rezultati ukazuju na moguću ulogu korijandera u budućim terapijskim pristupima infekcijama izazvanih gastrointestinalnim nematoda ovaca, nakon daljih in vivo ispitivanja i studija toksičnosti., The development of anthelmintic resistance in sheep gastrointestinal nematodes to commercial anthelmintics has led to enormous economic losses in many parts of the world. For this reason, new sustainable strategies for the control of these parasites are being actively searched, which include the use of different alternatives such as plant essential oils. The aim of this study was to determine the chemical composition of the essential oil of coriander (Coriandrum sativum L.) and to test its anthelmintic potential against gastrointestinal nematodes of sheep. Qualitative and semi-quantitative chemical characterization of the essential oil was performed using gas chromatography coupled with mass spectrometric detection (GC-MS analysis). The anthelmintic potential of the essential oil was tested using the in vitro egg hatch test (EHT), which was carried out at eight different concentrations of the tested oil (50; 12.5; 3.125; 0.781; 0.195; 0.049; 0.025 and 0.0125 mg/mL). Thiabendazole in concentrations of 0.025 and 0.0125 mg/mL was used as the positive control, and the emulsifier 3% Tween 80 v/v and distilled water was used as the negative control. GC-MS analyzes revealed a rich chemical composition of the tested oil, where a total of 17 different ingredients from different chemical groups were found, of which 15 were identified. The dominant ingredient was linalool (84.5%) along with γ-terpinene (5.04%), camphor (3.26%) and α-pinene (2.88%). The EHT results showed a high anthelmintic potential of coriander oil with inhibition of egg hatching, i.e. an ovicidal effect of 29.0-88.7% depending on the concentration, with an IC50 concentration of 0.04 mg/mL and a clear dose-dependent effect (R2=0.90). This study confirmed the possible importance of botanical anthelmintics in the sustainable control of nematodes in animals and the combatting anthelmintic resistance. The obtained results indicate a possible role of coriander in future therapeutic approaches to infections caused by gastrointestinal nematodes of sheep, after further in vivo tests and toxicity studies.",
publisher = "JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka",
journal = "Veterinarski Žurnal Republike Srpske",
title = "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca, Anthelmintic Potential of Coriander Essential Oil (Coriandrum sativum L.) in Sheep",
pages = "126-101",
number = "1-2",
volume = "XXII",
doi = "10.7251/VETJSR2201101S"
}

Štrbac, F., Bosco, A., Petrović, K., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Cringoli, G.,& Rinaldi, L.. (2022). Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca. in Veterinarski Žurnal Republike Srpske
JU Veterinarski institut Republike Srpske "Dr Vaso Butozan" Banja Luka., XXII(1-2), 101-126.
https://doi.org/10.7251/VETJSR2201101S

Štrbac F, Bosco A, Petrović K, Stojanović D, Ratajac R, Simin N, Orčić D, Cringoli G, Rinaldi L. Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca. in Veterinarski Žurnal Republike Srpske. 2022;XXII(1-2):101-126.
doi:10.7251/VETJSR2201101S .

Štrbac, Filip, Bosco, Antonio, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Cringoli, Giuseppe, Rinaldi, Laura, "Antihelmintički potencijal etarskog ulja korijandera (Coriandrum sativum L.) kod ovaca" in Veterinarski Žurnal Republike Srpske, XXII, no. 1-2 (2022):101-126,
https://doi.org/10.7251/VETJSR2201101S . .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Krnjajić, Slobodan
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Ratajac, Radomir
AU  - Petrović, Kosta
AU  - Knežević, Goran
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Bosco, Antonio
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1884
AB  - The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.
PB  - MDPI
T2  - Animals
T1  - A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep
IS  - 1
SP  - 45
VL  - 13
DO  - 10.3390/ani13010045
ER  - 

@article{
author = "Štrbac, Filip and Krnjajić, Slobodan and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Knežević, Goran and Cringoli, Giuseppe and Rinaldi, Laura and Bosco, Antonio",
year = "2022",
abstract = "The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of O. vulgare was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.",
publisher = "MDPI",
journal = "Animals",
title = "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep",
number = "1",
pages = "45",
volume = "13",
doi = "10.3390/ani13010045"
}

Štrbac, F., Krnjajić, S., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Knežević, G., Cringoli, G., Rinaldi, L.,& Bosco, A.. (2022). A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals
MDPI., 13(1), 45.
https://doi.org/10.3390/ani13010045

Štrbac F, Krnjajić S, Maurelli MP, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Knežević G, Cringoli G, Rinaldi L, Bosco A. A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep. in Animals. 2022;13(1):45.
doi:10.3390/ani13010045 .

Štrbac, Filip, Krnjajić, Slobodan, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Knežević, Goran, Cringoli, Giuseppe, Rinaldi, Laura, Bosco, Antonio, "A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep" in Animals, 13, no. 1 (2022):45,
https://doi.org/10.3390/ani13010045 . .

TY  - JOUR
AU  - Petrović, Kosta
AU  - Djokovic, Radojica
AU  - Cincović, Marko
AU  - Hristovska, Talija
AU  - Lalović, Miroslav
AU  - Petrović, Miloš
AU  - Majkić, Mira
AU  - Došenović Marinković, Maja
AU  - Anđušić, Ljiljana
AU  - Devečerski, Gordana
AU  - Stojanović, Dragica
AU  - Štrbac, Filip
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1883
AB  - Previous experimental models on cows have examined the difference in the metabolic adaptation in cows after niacin administration, without identifying the most important mediators between niacin administration and its biological effects, namely active forms of niacin. All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The aim of this study was to determine the influence of niacin administration in periparturient period on NAD, NADP and the NAD:NADP ratio and to determine relationship between these indicators of an active form of niacin with metabolic parameters in cow blood. The study included 90 healthy cows: 45 cows receiving niacin and 45 cows were negative control. The niacin group was treated with nicotinic acid for two weeks before, as well as two weeks after parturition. Nicotinic acid was applied per os with feed. In cows receiving niacin, there was a significantly higher concentration of NAD and NADP, but the NAD:NADP ratio did not differ compared with control. All three indicators were able to separate cows who received and who did not receive additional niacin. NAD and NADP are good indicators of the availability of niacin from additional sources. The NAD:NADP ratio is a good indicator of the biological effect of applied niacin on metabolites in cows due to its correlation with a number of metabolites: positive correlation with glucose, insulin, glucose to insulin ratio and the revised quantitative insulin sensitivity check index (RQUICKIBHB) of insulin resistance, triglycerides and cholesterol, and a negative correlation with nonesterified fatty acid (NEFA), beta hydroxybutyrate (BHB), gamma-glutamyltranspherase (GGT) and urea in cows receiving niacin. The same amount of added niacin in feed can produce different concentrations of NAD, NADP and NAD:NADP in the blood, and this was not related to their concentration before the addition of niacin. The change in the concentration of the active form of niacin (NAD, NADP and NAD:NADP) further correlates with the concentration of metabolic parameters, which indicates that the intensity of the biological effect of additional niacin can be accurately determined only if we know the concentrations of its active forms in blood. Under basal conditions (without additional niacin), active forms of niacin that already exist in the blood do not show significant correlations with metabolic parameters.
PB  - MDPI
T2  - Animals
T1  - Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation
IS  - 12
SP  - 1524
VL  - 12
DO  - 10.3390/ani12121524
ER  - 

@article{
author = "Petrović, Kosta and Djokovic, Radojica and Cincović, Marko and Hristovska, Talija and Lalović, Miroslav and Petrović, Miloš and Majkić, Mira and Došenović Marinković, Maja and Anđušić, Ljiljana and Devečerski, Gordana and Stojanović, Dragica and Štrbac, Filip",
year = "2022",
abstract = "Previous experimental models on cows have examined the difference in the metabolic adaptation in cows after niacin administration, without identifying the most important mediators between niacin administration and its biological effects, namely active forms of niacin. All tissues in the body convert absorbed niacin into its main metabolically active form, the coenzyme nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP). The aim of this study was to determine the influence of niacin administration in periparturient period on NAD, NADP and the NAD:NADP ratio and to determine relationship between these indicators of an active form of niacin with metabolic parameters in cow blood. The study included 90 healthy cows: 45 cows receiving niacin and 45 cows were negative control. The niacin group was treated with nicotinic acid for two weeks before, as well as two weeks after parturition. Nicotinic acid was applied per os with feed. In cows receiving niacin, there was a significantly higher concentration of NAD and NADP, but the NAD:NADP ratio did not differ compared with control. All three indicators were able to separate cows who received and who did not receive additional niacin. NAD and NADP are good indicators of the availability of niacin from additional sources. The NAD:NADP ratio is a good indicator of the biological effect of applied niacin on metabolites in cows due to its correlation with a number of metabolites: positive correlation with glucose, insulin, glucose to insulin ratio and the revised quantitative insulin sensitivity check index (RQUICKIBHB) of insulin resistance, triglycerides and cholesterol, and a negative correlation with nonesterified fatty acid (NEFA), beta hydroxybutyrate (BHB), gamma-glutamyltranspherase (GGT) and urea in cows receiving niacin. The same amount of added niacin in feed can produce different concentrations of NAD, NADP and NAD:NADP in the blood, and this was not related to their concentration before the addition of niacin. The change in the concentration of the active form of niacin (NAD, NADP and NAD:NADP) further correlates with the concentration of metabolic parameters, which indicates that the intensity of the biological effect of additional niacin can be accurately determined only if we know the concentrations of its active forms in blood. Under basal conditions (without additional niacin), active forms of niacin that already exist in the blood do not show significant correlations with metabolic parameters.",
publisher = "MDPI",
journal = "Animals",
title = "Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation",
number = "12",
pages = "1524",
volume = "12",
doi = "10.3390/ani12121524"
}

Petrović, K., Djokovic, R., Cincović, M., Hristovska, T., Lalović, M., Petrović, M., Majkić, M., Došenović Marinković, M., Anđušić, L., Devečerski, G., Stojanović, D.,& Štrbac, F.. (2022). Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation. in Animals
MDPI., 12(12), 1524.
https://doi.org/10.3390/ani12121524

Petrović K, Djokovic R, Cincović M, Hristovska T, Lalović M, Petrović M, Majkić M, Došenović Marinković M, Anđušić L, Devečerski G, Stojanović D, Štrbac F. Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation. in Animals. 2022;12(12):1524.
doi:10.3390/ani12121524 .

Petrović, Kosta, Djokovic, Radojica, Cincović, Marko, Hristovska, Talija, Lalović, Miroslav, Petrović, Miloš, Majkić, Mira, Došenović Marinković, Maja, Anđušić, Ljiljana, Devečerski, Gordana, Stojanović, Dragica, Štrbac, Filip, "Niacin Status Indicators and Their Relationship with Metabolic Parameters in Dairy Cows during Early Lactation" in Animals, 12, no. 12 (2022):1524,
https://doi.org/10.3390/ani12121524 . .

TY  - JOUR
AU  - Nikolić, Sandra
AU  - Stojanović, Dragica
AU  - Cincović, Marko
AU  - Majkić, Mira
AU  - Došenović Marinković, Maja
AU  - Spasojević, Jovan
AU  - Galić, Ivan
AU  - Štrbac, Filip
AU  - Kovačević, Dražen
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1886
AB  - Ivermektin je antiparazitski lek koji pripada familiji makrocikličnih laktona. Veoma je efikasan protiv endo- i ekto- parazita u niskim dozama, a u poslednje vreme se najčešće upotrebljava u prevenciji i lečenju bolesti srčanog crva. Cilj ovog istraživanja je bio utvrditi da li se prilikom dugotrajne primene ivermektina, u dozama od 10 μg/kg t.m., u lečenju pasa od srčanog crva javljaju promene u hematološkim i biohemijskim parametrima koje se mogu pripisati dejstvu leka. Studija je obuhvatala pse koji su primali ivermektin parenteralno na svake dve nedelje tokom šest meseci u terapiji srčanog crva. Krv je uzorkovana u momentu dijagnostikovanja bolesti kod psa, pre početka terapije, a zatim prilikom kontrolnog testiranja nakon završenih šest meseci terapije. Aplikacija ivermektina dva puta mesečno u dozi od 10 μg/kg t.m. u trajanju od šest meseci u cilju lečenja bolesti srčanog crva kod pasa dovodi do značajnog variranja pojedinih parametara posle terapije u odnosu na pre početka terapije. U hematološkom profilu ukupan broj limfocita i RDW pokazuju najveću varijabilnost koja se može pripisati dejstvu leka (53,33%). Zatim slede monociti (46,66%), hematokrit (40%), eritrociti i hemoglobin (33,33%), MCH i trombociti (26,66%) i na kraju leukociti, MCHC i neutrofili (13,33%). U biohemijskom profilu, variranje koje se može pripisati leku se javilo kod 60% pasa za parametre kreatinin, AST, trigliceridi i kalcijum, 46,66% za fosfor, 40% za ALT, 33,33% za ureu, 26,66% za ukupne proteine, 20% za albumine i 13,33% za glukozu, bilirubin, ALP i holesterol. Dobijeni rezultati ukazuju da je potrebno kontrolisati životinje prilikom dugotrajne primene ivermektina, obavestiti vlasnike o efektima leka tokom dugotrajne primene, a takođe treba razmotriti i upotrebu suplemenata koji ublažavaju neželjene efekte leka.
AB  - Ivermectin is an antiparasitic medicine that belongs to the macrocyclic lactone family. It is highly effective against endo- and ecto- parasites at low doses, and lately it is most widely used in the treatment and prophylaxis of canine heartworm disease. The aim of this study was to determine whether there is the occurrence of changes in hematological and biochemical blood parameters due to the long-term application of ivermectin, at doses of 10 μg/kg b.w., in heartworm disease treatment, that can be attributed to the effect of the medicine. This study was conducted on dogs with heartworm disease that received ivermectin twice a month for six months. Blood was collected at the moment of diagnosis, before treatment and after six months of therapy. The results showed that application of ivermectin twice a month, at doses of 10 μg/kg b.w., during a six-month period of heartworm treatment, induced significant variability of the certain parameters after the treatment in regard to before the treatment. The greatest variability in hematological findings that can be attributed to the effects of medicine was shown by total lymphocyte count and RDW (53.33%), monocyte count (46.66%), hematocrit (40%), total red blood cells count and hemoglobin (33.33%), MCH and platelets (26.66%), total white blood cells count, MCHC and neutrophils (13.33%), respectively. The variation that can be associated with the effects of ivermectin in biochemical parameters was demonstrated in 60% of the dogs for the parameters creatinine, AST, triglycerides and calcium, 46.66% phosphorus, 40% ALT, 33.33% urea, 26.66% total protein, 20% albumin, 13.33% glucose, bilirubin, ALP and cholesterol, respectively. These results point out that better control of animals should be provided during long-term use of ivermectin, also that the owners should be thoroughly informed about the effects of the medicine and that some consideration should be given to the use of supplements which could ease the side effects of ivermectin.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Letopis Naučnih Radova
T1  - Promene u hematološkim i biohemijskim parametrima krvi pasa prilikom dugotrajne primene ivermektina u cilju lečenja bolesti srčanog crva
T1  - Changes in Hematological and Biochemical Parameters of Canine Blood During Long-term Use of Ivermectin for the Treatment of Heartworm Disease
EP  - 10
IS  - 1
SP  - 1
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1886
ER  - 

@article{
author = "Nikolić, Sandra and Stojanović, Dragica and Cincović, Marko and Majkić, Mira and Došenović Marinković, Maja and Spasojević, Jovan and Galić, Ivan and Štrbac, Filip and Kovačević, Dražen",
year = "2022",
abstract = "Ivermektin je antiparazitski lek koji pripada familiji makrocikličnih laktona. Veoma je efikasan protiv endo- i ekto- parazita u niskim dozama, a u poslednje vreme se najčešće upotrebljava u prevenciji i lečenju bolesti srčanog crva. Cilj ovog istraživanja je bio utvrditi da li se prilikom dugotrajne primene ivermektina, u dozama od 10 μg/kg t.m., u lečenju pasa od srčanog crva javljaju promene u hematološkim i biohemijskim parametrima koje se mogu pripisati dejstvu leka. Studija je obuhvatala pse koji su primali ivermektin parenteralno na svake dve nedelje tokom šest meseci u terapiji srčanog crva. Krv je uzorkovana u momentu dijagnostikovanja bolesti kod psa, pre početka terapije, a zatim prilikom kontrolnog testiranja nakon završenih šest meseci terapije. Aplikacija ivermektina dva puta mesečno u dozi od 10 μg/kg t.m. u trajanju od šest meseci u cilju lečenja bolesti srčanog crva kod pasa dovodi do značajnog variranja pojedinih parametara posle terapije u odnosu na pre početka terapije. U hematološkom profilu ukupan broj limfocita i RDW pokazuju najveću varijabilnost koja se može pripisati dejstvu leka (53,33%). Zatim slede monociti (46,66%), hematokrit (40%), eritrociti i hemoglobin (33,33%), MCH i trombociti (26,66%) i na kraju leukociti, MCHC i neutrofili (13,33%). U biohemijskom profilu, variranje koje se može pripisati leku se javilo kod 60% pasa za parametre kreatinin, AST, trigliceridi i kalcijum, 46,66% za fosfor, 40% za ALT, 33,33% za ureu, 26,66% za ukupne proteine, 20% za albumine i 13,33% za glukozu, bilirubin, ALP i holesterol. Dobijeni rezultati ukazuju da je potrebno kontrolisati životinje prilikom dugotrajne primene ivermektina, obavestiti vlasnike o efektima leka tokom dugotrajne primene, a takođe treba razmotriti i upotrebu suplemenata koji ublažavaju neželjene efekte leka., Ivermectin is an antiparasitic medicine that belongs to the macrocyclic lactone family. It is highly effective against endo- and ecto- parasites at low doses, and lately it is most widely used in the treatment and prophylaxis of canine heartworm disease. The aim of this study was to determine whether there is the occurrence of changes in hematological and biochemical blood parameters due to the long-term application of ivermectin, at doses of 10 μg/kg b.w., in heartworm disease treatment, that can be attributed to the effect of the medicine. This study was conducted on dogs with heartworm disease that received ivermectin twice a month for six months. Blood was collected at the moment of diagnosis, before treatment and after six months of therapy. The results showed that application of ivermectin twice a month, at doses of 10 μg/kg b.w., during a six-month period of heartworm treatment, induced significant variability of the certain parameters after the treatment in regard to before the treatment. The greatest variability in hematological findings that can be attributed to the effects of medicine was shown by total lymphocyte count and RDW (53.33%), monocyte count (46.66%), hematocrit (40%), total red blood cells count and hemoglobin (33.33%), MCH and platelets (26.66%), total white blood cells count, MCHC and neutrophils (13.33%), respectively. The variation that can be associated with the effects of ivermectin in biochemical parameters was demonstrated in 60% of the dogs for the parameters creatinine, AST, triglycerides and calcium, 46.66% phosphorus, 40% ALT, 33.33% urea, 26.66% total protein, 20% albumin, 13.33% glucose, bilirubin, ALP and cholesterol, respectively. These results point out that better control of animals should be provided during long-term use of ivermectin, also that the owners should be thoroughly informed about the effects of the medicine and that some consideration should be given to the use of supplements which could ease the side effects of ivermectin.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Letopis Naučnih Radova",
title = "Promene u hematološkim i biohemijskim parametrima krvi pasa prilikom dugotrajne primene ivermektina u cilju lečenja bolesti srčanog crva, Changes in Hematological and Biochemical Parameters of Canine Blood During Long-term Use of Ivermectin for the Treatment of Heartworm Disease",
pages = "10-1",
number = "1",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1886"
}

Nikolić, S., Stojanović, D., Cincović, M., Majkić, M., Došenović Marinković, M., Spasojević, J., Galić, I., Štrbac, F.,& Kovačević, D.. (2022). Promene u hematološkim i biohemijskim parametrima krvi pasa prilikom dugotrajne primene ivermektina u cilju lečenja bolesti srčanog crva. in Letopis Naučnih Radova
Poljoprivredni fakultet, Univerzitet u Novom Sadu., 46(1), 1-10.
https://hdl.handle.net/21.15107/rcub_rimsi_1886

Nikolić S, Stojanović D, Cincović M, Majkić M, Došenović Marinković M, Spasojević J, Galić I, Štrbac F, Kovačević D. Promene u hematološkim i biohemijskim parametrima krvi pasa prilikom dugotrajne primene ivermektina u cilju lečenja bolesti srčanog crva. in Letopis Naučnih Radova. 2022;46(1):1-10.
https://hdl.handle.net/21.15107/rcub_rimsi_1886 .

Nikolić, Sandra, Stojanović, Dragica, Cincović, Marko, Majkić, Mira, Došenović Marinković, Maja, Spasojević, Jovan, Galić, Ivan, Štrbac, Filip, Kovačević, Dražen, "Promene u hematološkim i biohemijskim parametrima krvi pasa prilikom dugotrajne primene ivermektina u cilju lečenja bolesti srčanog crva" in Letopis Naučnih Radova, 46, no. 1 (2022):1-10,
https://hdl.handle.net/21.15107/rcub_rimsi_1886 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Maurelli, Maria Paola
AU  - Ratajac, Radomir
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orcic, Dejan
AU  - Pusic, Ivan
AU  - Krnjajić, Slobodan
AU  - Sotiraki, Smaragda
AU  - Saralli, Giorgio
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1548
AB  - Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.
PB  - MDPI, Basel
T2  - Veterinary Sciences
T1  - Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies
IS  - 2
VL  - 9
DO  - 10.3390/vetsci9020093
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Maurelli, Maria Paola and Ratajac, Radomir and Stojanović, Dragica and Simin, Nataša and Orcic, Dejan and Pusic, Ivan and Krnjajić, Slobodan and Sotiraki, Smaragda and Saralli, Giorgio and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "Herbal products such as essential oils may play a promising role in the treatment of infections caused by gastrointestinal nematodes (GINs). The aim of this study was to evaluate the in vitro potential of 11 essential oils (EOs) and one binary combination of isolated EO compounds, as well as the in vivo anthelmintic efficacy of two EO formulations. Four GIN genera were identified in the coproculture examination: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The in vitro egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO, whereas in the in vivo faecal egg count reduction test (FECRT), each EO sample was diluted in sunflower oil and orally administrated at a dose of 100 mg/kg to the different group of animals. In the EHT, the EOs of Origanum vulgare, Foeniculum vulgare, Satureja montana, Satureja hortensis and two types of Thymus vulgaris were the most effective. The dominant compounds of these EOs were carvacrol, thymol, anethol, p-cymene and gamma-terpinene, indicating their importance for the anthelmintic activity. In the FECRT, both T. vulgaris EO type 1 and linalool:estragole combination show an anthelmintic potential with a mean effect on FECR of approximately 25%. The results suggest the possible role of tested EOs as anthelmintic agents in sheep farms, although further in vivo tests are needed.",
publisher = "MDPI, Basel",
journal = "Veterinary Sciences",
title = "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies",
number = "2",
volume = "9",
doi = "10.3390/vetsci9020093"
}

Štrbac, F., Bosco, A., Maurelli, M. P., Ratajac, R., Stojanović, D., Simin, N., Orcic, D., Pusic, I., Krnjajić, S., Sotiraki, S., Saralli, G., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences
MDPI, Basel., 9(2).
https://doi.org/10.3390/vetsci9020093

Štrbac F, Bosco A, Maurelli MP, Ratajac R, Stojanović D, Simin N, Orcic D, Pusic I, Krnjajić S, Sotiraki S, Saralli G, Cringoli G, Rinaldi L. Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies. in Veterinary Sciences. 2022;9(2).
doi:10.3390/vetsci9020093 .

Štrbac, Filip, Bosco, Antonio, Maurelli, Maria Paola, Ratajac, Radomir, Stojanović, Dragica, Simin, Nataša, Orcic, Dejan, Pusic, Ivan, Krnjajić, Slobodan, Sotiraki, Smaragda, Saralli, Giorgio, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Essential Oils to Control Gastrointestinal Nematodes in Sheep-In Vitro and In Vivo Studies" in Veterinary Sciences, 9, no. 2 (2022),
https://doi.org/10.3390/vetsci9020093 . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1972
AB  - Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.
PB  - World Association for the Advancement of Veterinary Parasitology (WAAVP)
C3  - Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
T1  - In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes
EP  - 517
SP  - 517
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1972
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.",
publisher = "World Association for the Advancement of Veterinary Parasitology (WAAVP)",
journal = "Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland",
title = "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes",
pages = "517-517",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1972"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
World Association for the Advancement of Veterinary Parasitology (WAAVP)., 517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland. 2021;:517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes" in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland (2021):517-517,
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1975
AB  - Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
T1  - Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties
EP  - 161
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1975
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"",
title = "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties",
pages = "161-160",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1975"
}

Štrbac, F., Simin, N., Orčić, D., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975

Štrbac F, Simin N, Orčić D, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Pušić I, Krnjajić S, Ratajac R. Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms". 2021;:160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties" in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms" (2021):160-161,
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1909
AB  - Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Због тога се ови биљни производи од давнина користе у народној медицини многих култура широм света, а могли би наћи широку примену и у ветеринарској медицини. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразатских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине које их чине погодним за употребу, као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације већ користе, као што су шампони на природној бази који најчешће садрже више различитих етарских уља или њихових састојака у оптималној сразмери. Међутим, главно ограничење за употребу ових биљних производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највеероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП- глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова ефикасност као и омогућити контролисано ослобађање, што све заједно може допринети смањењу потребне дозе. На тај начин се може додатно повећати сигурност њихове примене.
AB  - Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. These herbal products have long utilized in folk medicine in many cultures around the world, and also could be widely used in veterinary medicine. However, little is known about their use in animals including dogs and cats, although researches so far highlighted different possible application. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties that make them suitable for use, such as rich chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain formulations are already in use, such as natural shampoos that usually contain several different essential oils or their ingredients in optimal proportions. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that participate in metabolic processes (eg UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the way of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their efficacy as well as enable controlled release, which all may contribute to reducing the required dose. In this way, the safety of their application can be further increased.
PB  - Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске
C3  - Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
T1  - Могућност и ограничења примене етарских уља код паса и мачака
T1  - Possibility and Limitations of the Use of Essential Oils in Dogs and Cats
EP  - 202
SP  - 201
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1909
ER  - 

@conference{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir",
year = "2021",
abstract = "Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Због тога се ови биљни производи од давнина користе у народној медицини многих култура широм света, а могли би наћи широку примену и у ветеринарској медицини. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразатских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине које их чине погодним за употребу, као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације већ користе, као што су шампони на природној бази који најчешће садрже више различитих етарских уља или њихових састојака у оптималној сразмери. Међутим, главно ограничење за употребу ових биљних производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највеероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП- глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова ефикасност као и омогућити контролисано ослобађање, што све заједно може допринети смањењу потребне дозе. На тај начин се може додатно повећати сигурност њихове примене., Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. These herbal products have long utilized in folk medicine in many cultures around the world, and also could be widely used in veterinary medicine. However, little is known about their use in animals including dogs and cats, although researches so far highlighted different possible application. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties that make them suitable for use, such as rich chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain formulations are already in use, such as natural shampoos that usually contain several different essential oils or their ingredients in optimal proportions. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that participate in metabolic processes (eg UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the way of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their efficacy as well as enable controlled release, which all may contribute to reducing the required dose. In this way, the safety of their application can be further increased.",
publisher = "Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске",
journal = "Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)",
title = "Могућност и ограничења примене етарских уља код паса и мачака, Possibility and Limitations of the Use of Essential Oils in Dogs and Cats",
pages = "202-201",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1909"
}

Štrbac, F., Petrović, K., Stojanović, D.,& Ratajac, R.. (2021). Могућност и ограничења примене етарских уља код паса и мачака. in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина)
Ветеринарска комора Републике Српске и Друштво ветеринара Републике Српске., 201-202.
https://hdl.handle.net/21.15107/rcub_rimsi_1909

Štrbac F, Petrović K, Stojanović D, Ratajac R. Могућност и ограничења примене етарских уља код паса и мачака. in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина). 2021;:201-202.
https://hdl.handle.net/21.15107/rcub_rimsi_1909 .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, "Могућност и ограничења примене етарских уља код паса и мачака" in Зборник кратких садржаја 26. Годишњег савјетовања доктора ветеринарске медицине Републике Српске (Босна и Херцеговина) (2021):201-202,
https://hdl.handle.net/21.15107/rcub_rimsi_1909 .

TY  - JOUR
AU  - Stojanović, Dragica
AU  - Cincović, Marko
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - CRISTINA, ROMEO TEODOR
AU  - Kovačević, Zorana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1880
AB  - Sepsis can be defined as a multisystem response of the organism on bacterial infection agents. The aim of this study is to provides an extensive analysis of associations between numerous routine biochemical and hematological markers in function of type of sepsis caused by experimental Gram-positive (G+, Staphylococcus aureus), Gram-negative (G-, Escherichia coli) and mixed (MIX) microorganism in laboratory rats model. In G- and G+ sepsis higher values of erythrocytes, total protein, albumin, creatinine, sodium and plasma osmolarity were noted and lower triglycerides values compared to MIX sepsis. Existence of statistically significant interaction between time and type of sepsis was showed that values of erythrocytes, neutrophiles, LUC, glucose, total protein, urea, Na, Cl, osmolarity and LDH were changed during time in function of sepsis type. Distinguish of animals with G+, G- or MIX sepsis is not possible because principal component analysis (PCA) demonstrated similar expression of blood hematological and metabolic parameters in animals regardless type of experimental sepsis (G+, G- or MIX), with minimal deviation of animal with MIX sepsis from animals with sepsis caused by pure culture (G+ or G-). Correlation coefficient analysis showed 507 significant correlations between routine blood parameters (207 in G-, 186 in G+ and 114 in MIX) and some of them are in function of type of sepsis. Distinguish of type of sepsis in function of blood parameters is not possible. After extraction of two components they load by blood parameters was similar in all type of sepsis (positive load with different type of white blood cells, PLT, glucose, amylase, Cl, urea and osmolarity, and negative load with albumin, total protein, cholesterol, creatinine and ALT). Although our results demonstrated difference in values and correlation coefficients for routine blood biochemistry and hematology parameters, multiparametric statistic showed they not be a useful tool for distinguishing type of sepsis according bacterial causative.
AB  - Sepsa se može definisati kao multisistemski odgovor organizma na uzročnike bakterijske infekcije. Cilj ove studije je da pruži opsežnu analizu povezanosti brojnih rutinskih biohemijskih i hematoloških markera u funkciji tipa sepse izazvane eksperimentalnim Gram-pozitivnim (G+, Staphylococcus aureus), Gramnegativnim (G-, Escherichia coli) i mešani (MIX) mikroorganizam u modelu laboratorijskih pacova. U G- i G+ sepsi su zabeležene više vrednosti eritrocita, ukupnog proteina, albumina, kreatinina, natrijuma i osmolarnosti plazme i niže vrednosti triglicerida u odnosu na MIX sepsu. Utvrđeno je postojanje statistički značajne interakcije između vremena i tipa sepse da su se vrednosti eritrocita, neutrofila, LUC, glukoze, ukupnog proteina, uree, Na, Cl, osmolarnosti i LDH menjale tokom vremena u funkciji tipa sepse. Razlikovanje životinja sa G+, G- ili MIX sepsom nije moguće jer je analiza glavnih komponenti (PCA) pokazala sličnu ekspresiju hematoloških i metaboličkih parametara krvi kod životinja bez obzira na tip eksperimentalne sepse (G+, G- ili MIX), uz minimalno odstupanje od životinja sa MIX sepsom od životinja sa sepsom izazvanom čistom kulturom (G+ ili G-). Analiza koeficijenta korelacije pokazala je 507 značajnih korelacija između rutinskih parametara krvi (207 kod EC, 186 kod SA i 114 kod MIX sepse) i neke od njih su u funkciji tipa sepse. Nije moguće razlikovati vrstu sepse u funkciji parametara krvi. Nakon ekstrakcije dve komponente opterećenje po parametrima krvi bilo je slično kod svih vrsta sepse (pozitivno opterećenje različitim vrstama belih krvnih zrnaca, PLT, glukozom, amilazom, Cl, ureom i osmolarnošću, a negativno albuminom, ukupnim proteinom, holesterolom , kreatinin i ALT). Iako su naši rezultati pokazali razliku u vrednostima i koeficijentima korelacije za rutinske biohemijske i hematološke parametre krvi, multiparametarska statistika je pokazala da oni nisu korisno sredstvo za razlikovanje tipa sepse prema bakterijskom uzročniku.
PB  - Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Letopis Naučnih Radova
T1  - Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats
T1  - Analiza glavnih komponenti i korelacije rutinskih parametara krvi u eksperimentalnoj sepsi kod pacova
EP  - 152
IS  - 2
SP  - 143
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1880
ER  - 

@article{
author = "Stojanović, Dragica and Cincović, Marko and Štrbac, Filip and Petrović, Kosta and CRISTINA, ROMEO TEODOR and Kovačević, Zorana",
year = "2021",
abstract = "Sepsis can be defined as a multisystem response of the organism on bacterial infection agents. The aim of this study is to provides an extensive analysis of associations between numerous routine biochemical and hematological markers in function of type of sepsis caused by experimental Gram-positive (G+, Staphylococcus aureus), Gram-negative (G-, Escherichia coli) and mixed (MIX) microorganism in laboratory rats model. In G- and G+ sepsis higher values of erythrocytes, total protein, albumin, creatinine, sodium and plasma osmolarity were noted and lower triglycerides values compared to MIX sepsis. Existence of statistically significant interaction between time and type of sepsis was showed that values of erythrocytes, neutrophiles, LUC, glucose, total protein, urea, Na, Cl, osmolarity and LDH were changed during time in function of sepsis type. Distinguish of animals with G+, G- or MIX sepsis is not possible because principal component analysis (PCA) demonstrated similar expression of blood hematological and metabolic parameters in animals regardless type of experimental sepsis (G+, G- or MIX), with minimal deviation of animal with MIX sepsis from animals with sepsis caused by pure culture (G+ or G-). Correlation coefficient analysis showed 507 significant correlations between routine blood parameters (207 in G-, 186 in G+ and 114 in MIX) and some of them are in function of type of sepsis. Distinguish of type of sepsis in function of blood parameters is not possible. After extraction of two components they load by blood parameters was similar in all type of sepsis (positive load with different type of white blood cells, PLT, glucose, amylase, Cl, urea and osmolarity, and negative load with albumin, total protein, cholesterol, creatinine and ALT). Although our results demonstrated difference in values and correlation coefficients for routine blood biochemistry and hematology parameters, multiparametric statistic showed they not be a useful tool for distinguishing type of sepsis according bacterial causative., Sepsa se može definisati kao multisistemski odgovor organizma na uzročnike bakterijske infekcije. Cilj ove studije je da pruži opsežnu analizu povezanosti brojnih rutinskih biohemijskih i hematoloških markera u funkciji tipa sepse izazvane eksperimentalnim Gram-pozitivnim (G+, Staphylococcus aureus), Gramnegativnim (G-, Escherichia coli) i mešani (MIX) mikroorganizam u modelu laboratorijskih pacova. U G- i G+ sepsi su zabeležene više vrednosti eritrocita, ukupnog proteina, albumina, kreatinina, natrijuma i osmolarnosti plazme i niže vrednosti triglicerida u odnosu na MIX sepsu. Utvrđeno je postojanje statistički značajne interakcije između vremena i tipa sepse da su se vrednosti eritrocita, neutrofila, LUC, glukoze, ukupnog proteina, uree, Na, Cl, osmolarnosti i LDH menjale tokom vremena u funkciji tipa sepse. Razlikovanje životinja sa G+, G- ili MIX sepsom nije moguće jer je analiza glavnih komponenti (PCA) pokazala sličnu ekspresiju hematoloških i metaboličkih parametara krvi kod životinja bez obzira na tip eksperimentalne sepse (G+, G- ili MIX), uz minimalno odstupanje od životinja sa MIX sepsom od životinja sa sepsom izazvanom čistom kulturom (G+ ili G-). Analiza koeficijenta korelacije pokazala je 507 značajnih korelacija između rutinskih parametara krvi (207 kod EC, 186 kod SA i 114 kod MIX sepse) i neke od njih su u funkciji tipa sepse. Nije moguće razlikovati vrstu sepse u funkciji parametara krvi. Nakon ekstrakcije dve komponente opterećenje po parametrima krvi bilo je slično kod svih vrsta sepse (pozitivno opterećenje različitim vrstama belih krvnih zrnaca, PLT, glukozom, amilazom, Cl, ureom i osmolarnošću, a negativno albuminom, ukupnim proteinom, holesterolom , kreatinin i ALT). Iako su naši rezultati pokazali razliku u vrednostima i koeficijentima korelacije za rutinske biohemijske i hematološke parametre krvi, multiparametarska statistika je pokazala da oni nisu korisno sredstvo za razlikovanje tipa sepse prema bakterijskom uzročniku.",
publisher = "Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Letopis Naučnih Radova",
title = "Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats, Analiza glavnih komponenti i korelacije rutinskih parametara krvi u eksperimentalnoj sepsi kod pacova",
pages = "152-143",
number = "2",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1880"
}

Stojanović, D., Cincović, M., Štrbac, F., Petrović, K., CRISTINA, R. T.,& Kovačević, Z.. (2021). Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats. in Letopis Naučnih Radova
Poljoprivredni fakultet, Univerzitet u Novom Sadu., 45(2), 143-152.
https://hdl.handle.net/21.15107/rcub_rimsi_1880

Stojanović D, Cincović M, Štrbac F, Petrović K, CRISTINA RT, Kovačević Z. Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats. in Letopis Naučnih Radova. 2021;45(2):143-152.
https://hdl.handle.net/21.15107/rcub_rimsi_1880 .

Stojanović, Dragica, Cincović, Marko, Štrbac, Filip, Petrović, Kosta, CRISTINA, ROMEO TEODOR, Kovačević, Zorana, "Principal Component and Correlation Analysis of Blood Routine Parameters in Experimental Sepsis in Rats" in Letopis Naučnih Radova, 45, no. 2 (2021):143-152,
https://hdl.handle.net/21.15107/rcub_rimsi_1880 .

TY  - JOUR
AU  - Petrović, Kosta
AU  - Štrbac, Filip
AU  - Stojanović, Dragica
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1887
AB  - Peripartalni period kod krava predstavlja stanje metaboličkog stresa koje se karakteriše različitim negativnim efektima. U ovom radu su opisani inflamatorni procesi kod krava u peripartalnom periodu. Naime, opisano je delovanje citokina, kao i interakcija citokina, metabolita i jetre kao centralnog metaboličkog organa. Prikazana je inflamacija tkiva u peripartalnom periodu sa posebnim osvrtom na značaj faktora nekroze tumora alfa (TNF-α). U tom kontekstu, ispitan je uticaj aplikacije niacina kod krava na regulisanje inflamatornog odgovora kroz merenje vrednosti TNF-α, haptoglobina i fibrinogena i poređenje sa kontrolnom grupom. Smanjenje koncentracije TNF-α i haptoglobina pod uticajem niacina ukazuje na njegov antiinflamatorni efekat, što sugeriše na značaj njegove primene kod krava u peripartalnom periodu.
AB  - The peripartum period in cows represents a state of metabolic stress characterized by various negative effects. This paper describes the inflammatory processes in cows during the peripartum period. Namely, it describes the action of cytokines, as well as the interactions of cytokines, metabolites and the liver as the central metabolic organ. Tissue inflammation in the peripartum period is presented with a particular focus on the importance of tumor necrosis factor alpha (TNF-α). Within this context, the influence of niacin application in cows on the regulation of the inflammatory response was examined by measuring the values of the TNF-α, haptoglobin and fibrinogen and comparing it with the control group. The decrease in the concentration of TNF-α and haptoglobin under the influence of niacin indicates its anti-inflammatory effects, which suggests the importance of its use in cows in the peripartum period.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu
T1  - Influence of Niacin Application on TNF-α, Haptoglobin and Fibrinogen Values in Cows in Peripartal Period
EP  - 32
IS  - 1
SP  - 26
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1887
ER  - 

@article{
author = "Petrović, Kosta and Štrbac, Filip and Stojanović, Dragica",
year = "2021",
abstract = "Peripartalni period kod krava predstavlja stanje metaboličkog stresa koje se karakteriše različitim negativnim efektima. U ovom radu su opisani inflamatorni procesi kod krava u peripartalnom periodu. Naime, opisano je delovanje citokina, kao i interakcija citokina, metabolita i jetre kao centralnog metaboličkog organa. Prikazana je inflamacija tkiva u peripartalnom periodu sa posebnim osvrtom na značaj faktora nekroze tumora alfa (TNF-α). U tom kontekstu, ispitan je uticaj aplikacije niacina kod krava na regulisanje inflamatornog odgovora kroz merenje vrednosti TNF-α, haptoglobina i fibrinogena i poređenje sa kontrolnom grupom. Smanjenje koncentracije TNF-α i haptoglobina pod uticajem niacina ukazuje na njegov antiinflamatorni efekat, što sugeriše na značaj njegove primene kod krava u peripartalnom periodu., The peripartum period in cows represents a state of metabolic stress characterized by various negative effects. This paper describes the inflammatory processes in cows during the peripartum period. Namely, it describes the action of cytokines, as well as the interactions of cytokines, metabolites and the liver as the central metabolic organ. Tissue inflammation in the peripartum period is presented with a particular focus on the importance of tumor necrosis factor alpha (TNF-α). Within this context, the influence of niacin application in cows on the regulation of the inflammatory response was examined by measuring the values of the TNF-α, haptoglobin and fibrinogen and comparing it with the control group. The decrease in the concentration of TNF-α and haptoglobin under the influence of niacin indicates its anti-inflammatory effects, which suggests the importance of its use in cows in the peripartum period.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu, Influence of Niacin Application on TNF-α, Haptoglobin and Fibrinogen Values in Cows in Peripartal Period",
pages = "32-26",
number = "1",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1887"
}

Petrović, K., Štrbac, F.,& Stojanović, D.. (2021). Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 2(1), 26-32.
https://hdl.handle.net/21.15107/rcub_rimsi_1887

Petrović K, Štrbac F, Stojanović D. Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu. in Veterinarski Pregled. 2021;2(1):26-32.
https://hdl.handle.net/21.15107/rcub_rimsi_1887 .

Petrović, Kosta, Štrbac, Filip, Stojanović, Dragica, "Uticaj aplikacije niacina na vrednosti TNF-α, haptoglobina i fibrinogena kod krava u peripartalnom periodu" in Veterinarski Pregled, 2, no. 1 (2021):26-32,
https://hdl.handle.net/21.15107/rcub_rimsi_1887 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Petrović, Kosta
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1881
AB  - Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразитских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације на бази етарских уља већ користе, попут различитих шампона. Међутим, главно ограничење за употребу ових производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП – глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова in vivo ефикасност, као и омогућити контролисано ослобађање. На тај начин се може смањити потребна доза и додатно повећати сигурност примене.
AB  - Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. Although researches have highlighted different possible application of these oils, little is known about their use in animals including dogs and cats. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties such as complex chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain essential oils based formulations are already in use, such as different shampoos. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that are involved in metabolic processes (as UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the methods of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their in vivo efficacy as well as enable controlled release. That can reduce the required dose and additionally increase safety of their application.
PB  - ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука
T2  - Ветеринарски Журнал Републике Српске
T1  - Могућности и ограничења примене етарских уља код паса и мачака
T1  - Possibilities and Limitations of the Use of Essential Oils in Dogs and Cats
EP  - 265
IS  - 1-2
SP  - 238
VL  - XXI
DO  - 10.7251/VETJEN2101238S
ER  - 

@article{
author = "Štrbac, Filip and Petrović, Kosta and Stojanović, Dragica and Ratajac, Radomir",
year = "2021",
abstract = "Етарска уља поседују бројна лековита својства која укључују антисептичко, антиинфламаторно, антиканцерогено али и антивирусно, антимикробно и антипаразитско деловање. Међутим, мало је познато о употреби етарских уља код животиња укључујући псе и мачке, иако досадашња истраживања говоре у прилог различитим могућностима њихове примене. Тако су етарска уља показала ефикасност против различитих бактеријских (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), гљивичних (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), ендопаразитских (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) и ектопаразитских (Otodectes cynotis, Demodex canis, Ctenocephalides felis, неки крпељи и др.) патогена код паса и мачака. При томе, етарска уља поседују бројне позитивне особине као што су богат хемијски састав (висока ефикасност, мања подложност резистенцији), природно порекло итд. Због тога се одређене формулације на бази етарских уља већ користе, попут различитих шампона. Међутим, главно ограничење за употребу ових производа јесте недовољно испитана безбедност примене. Тако се нека етарска уља сматрају токсичним за кућне љубимце, што се посебно односи на мачке. Главни разлог за то је највероватније дефицит појединих ензима јетре који учествују у метаболичким процесима (нпр. УДП – глукуронозилтрансфераза код мачака), што доводи до акумулације појединих активних састојака у организму и њихове токсичности. Због тога је потребан опрез и рационална примена у смислу адекватног одабира биљних врста, дозирања, концентрације и начина примене. Такође, с обзиром да су етарска уља склона испаравању и нестабилности, техником инкапсулације се може додатно повећати њихова in vivo ефикасност, као и омогућити контролисано ослобађање. На тај начин се може смањити потребна доза и додатно повећати сигурност примене., Essential oils have numerous medicinal properties which include antiseptic, anti-inflammatory, anticancer but also antiviral, antimicrobial and antiparasitic effects. Although researches have highlighted different possible application of these oils, little is known about their use in animals including dogs and cats. Specifically, essential oils showed effects against various bacterial (Staphylococcus spp., Pseudomonas aeruginosa, Enterococcus spp.), fungal (Malassezia pachydermatis, Microsporum canis, Trichophyton mentagrophytes), endoparasitic (Giardia duodenalis, Echinococcus granulosus, Toxocara canis) and ectoparasitic (Otodectes cynotis, Demodex canis, Ctenocephalides felis, some ticks etc) pathogens in dogs and cats. In addition, essential oils exhibit numerous positive properties such as complex chemical composition (high efficacy, less susceptibility to resistance), their natural origin etc. Therefore, certain essential oils based formulations are already in use, such as different shampoos. However, the main limitation for the use of these plant products is the insufficiently tested safety of use. Thus, some essential oils are considered toxic to pets, especially when used on cats. The main reason for this is most likely the deficiency of some liver enzymes that are involved in metabolic processes (as UDP-glucuronosyltransferase in cats), which leads to the accumulation of some active ingredients in the organism and their toxicity. Therefore, caution and rational application are needed in terms of adequate selection of plant species, dosage, concentration and the methods of use. In addition, since essential oils are prone to evaporation and instability, the encapsulation technique can further increase their in vivo efficacy as well as enable controlled release. That can reduce the required dose and additionally increase safety of their application.",
publisher = "ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука",
journal = "Ветеринарски Журнал Републике Српске",
title = "Могућности и ограничења примене етарских уља код паса и мачака, Possibilities and Limitations of the Use of Essential Oils in Dogs and Cats",
pages = "265-238",
number = "1-2",
volume = "XXI",
doi = "10.7251/VETJEN2101238S"
}

Štrbac, F., Petrović, K., Stojanović, D.,& Ratajac, R.. (2021). Могућности и ограничења примене етарских уља код паса и мачака. in Ветеринарски Журнал Републике Српске
ЈУ Ветеринарски институт Републике Српске "Др Васо Бутозан" Бања Лука., XXI(1-2), 238-265.
https://doi.org/10.7251/VETJEN2101238S

Štrbac F, Petrović K, Stojanović D, Ratajac R. Могућности и ограничења примене етарских уља код паса и мачака. in Ветеринарски Журнал Републике Српске. 2021;XXI(1-2):238-265.
doi:10.7251/VETJEN2101238S .

Štrbac, Filip, Petrović, Kosta, Stojanović, Dragica, Ratajac, Radomir, "Могућности и ограничења примене етарских уља код паса и мачака" in Ветеринарски Журнал Републике Српске, XXI, no. 1-2 (2021):238-265,
https://doi.org/10.7251/VETJEN2101238S . .