Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties
2023
Аутори
Štrbac, FilipSimin, Nataša
Orčić, Dejan
Krnjajić, Slobodan
Bosco, Antonio
Stojanović, Dragica
Ratajac, Radomir
Cringoli, Giuseppe
Rinaldi, Laura
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13....0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic
potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.
Кључне речи:
essential oils / peppermint / spearmint / chemical composition / anthelmintic activityИзвор:
Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”, 2023, 113-113Издавач:
- Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
Финансирање / пројекти:
- Ministry of Education, Science and Technological Development of the Republic of Serbia (Rescript no. 451-03-1183/2021-14)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Štrbac, Filip AU - Simin, Nataša AU - Orčić, Dejan AU - Krnjajić, Slobodan AU - Bosco, Antonio AU - Stojanović, Dragica AU - Ratajac, Radomir AU - Cringoli, Giuseppe AU - Rinaldi, Laura PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2137 AB - Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode. PB - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society C3 - Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology” T1 - Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties EP - 113 SP - 113 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2137 ER -
@conference{ author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Krnjajić, Slobodan and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Cringoli, Giuseppe and Rinaldi, Laura", year = "2023", abstract = "Plants of the genus Mentha are well-known for their various medicinal properties including anti-inflammatory, antiemetic, antispasmodic, analgesic and antiparasitic effects, which are used for the treatment of various gastrointestinal and respiratory diseases. The aim of this study was to determine the chemical composition of the essential oils (EOs) of two Mentha species, peppermint (M. piperita) and spearmint (M. spicata), and to evaluate their anthelmintic activity against gastrointestinal nematodes, parasites that have a significantly negative impact on modern sheep farming. The main compounds of peppermint EO, determined by GC-MS analyses, were menthol (32.6%), menthone (22.0%) and isomenthone (9.39%), and those of spearmint were carvone (64.4%), trans-4-caranone (8.67%) and limonene (4.37%). Their anthelmintic effects, assesed using the egg hatch test conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/ml), were 20.0-90.3% and 13.0-93.7%, respectively. Although both tested samples showed high and dose-dependent (R2 = 0.93 and 0.96, respectively) anthelmintic potential, their effect was significantly different at five concentrations (p<0.05). The obtained results suggest the high influence of differences in chemical composition of EOs on their pharmacological properties, although the samples were extracted from similar plant species. These should not be neglected during the preparation of formulation, which is important for finding alternatives to combat resistance in nematode.", publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society", journal = "Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”", title = "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties", pages = "113-113", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2137" }
Štrbac, F., Simin, N., Orčić, D., Krnjajić, S., Bosco, A., Stojanović, D., Ratajac, R., Cringoli, G.,& Rinaldi, L.. (2023). Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology” Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 113-113. https://hdl.handle.net/21.15107/rcub_rimsi_2137
Štrbac F, Simin N, Orčić D, Krnjajić S, Bosco A, Stojanović D, Ratajac R, Cringoli G, Rinaldi L. Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties. in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology”. 2023;:113-113. https://hdl.handle.net/21.15107/rcub_rimsi_2137 .
Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Krnjajić, Slobodan, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Cringoli, Giuseppe, Rinaldi, Laura, "Differences in Chemical Composition of the Essential Oils of Peppermint (Mentha x piperita L.) and Spearmint (Mentha spicata L.) and their Anthelmintic Properties" in Serbian Biochemical Society Twelfth Conference (international scientific meeting) - “Biochemistry in Biotechnology” (2023):113-113, https://hdl.handle.net/21.15107/rcub_rimsi_2137 .