TY  - CHAP
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Pušić, Ivan
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
AU  - Ratajac, Radomir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1900
AB  - Gastrointestinal nematodes (GINs) nowadays present a serious threat to sustainable sheep breeding. Various classes of commercial anthelmintics were used to control these parasites. However, due to the development of anthelmintic resistance (AR), the efficacy of these drugs has decreased, which in turn resulted in high economic losses. For these reasons, researchers are focused now on designing sustainable strategies for GIN control, based on the use of a combination of a bunch of options including the wise application of anthelmintic drugs, as well as applying alternative strategies. Within this context, phytotherapy (the use of plants or their products) presents one of the most promising alternatives. Essential oils (EOs) are natural, volatile and complex compounds characterized by a strong odor and extracted from aromatic plants. In various studies so far, these plant products showed high in vitro and, in some cases, in vivo efficacy against sheep GINs. The aim of this chapter is to review so far conducted studies based on the use of EOs against these parasites and to discuss results, as well as advantages of their use compared to commercial anthelmintics. On the other hand, current obstacles in the use of EOs and possible solutions on how to overcome them will be also discussed in this chapter. In this way, current and future perspectives of the use of EOs against sheep GINs are discussed here.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
T2  - Animal Health Perspectives
T1  - The Use of Essential Oils against Sheep Gastrointestinal Nematodes
EP  - 94
IS  - 12
SP  - 86
VL  - 1
DO  - 10.47278/book.ahp/2022.12
ER  - 

@inbook{
author = "Štrbac, Filip and Bosco, Antonio and Pušić, Ivan and Stojanović, Dragica and Simin, Nataša and Cringoli, Giuseppe and Rinaldi, Laura and Ratajac, Radomir",
year = "2022",
abstract = "Gastrointestinal nematodes (GINs) nowadays present a serious threat to sustainable sheep breeding. Various classes of commercial anthelmintics were used to control these parasites. However, due to the development of anthelmintic resistance (AR), the efficacy of these drugs has decreased, which in turn resulted in high economic losses. For these reasons, researchers are focused now on designing sustainable strategies for GIN control, based on the use of a combination of a bunch of options including the wise application of anthelmintic drugs, as well as applying alternative strategies. Within this context, phytotherapy (the use of plants or their products) presents one of the most promising alternatives. Essential oils (EOs) are natural, volatile and complex compounds characterized by a strong odor and extracted from aromatic plants. In various studies so far, these plant products showed high in vitro and, in some cases, in vivo efficacy against sheep GINs. The aim of this chapter is to review so far conducted studies based on the use of EOs against these parasites and to discuss results, as well as advantages of their use compared to commercial anthelmintics. On the other hand, current obstacles in the use of EOs and possible solutions on how to overcome them will be also discussed in this chapter. In this way, current and future perspectives of the use of EOs against sheep GINs are discussed here.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "Animal Health Perspectives",
booktitle = "The Use of Essential Oils against Sheep Gastrointestinal Nematodes",
pages = "94-86",
number = "12",
volume = "1",
doi = "10.47278/book.ahp/2022.12"
}

Štrbac, F., Bosco, A., Pušić, I., Stojanović, D., Simin, N., Cringoli, G., Rinaldi, L.,& Ratajac, R.. (2022). The Use of Essential Oils against Sheep Gastrointestinal Nematodes. in Animal Health Perspectives
Unique Scientific Publishers, Faisalabad, Pakistan., 1(12), 86-94.
https://doi.org/10.47278/book.ahp/2022.12

Štrbac F, Bosco A, Pušić I, Stojanović D, Simin N, Cringoli G, Rinaldi L, Ratajac R. The Use of Essential Oils against Sheep Gastrointestinal Nematodes. in Animal Health Perspectives. 2022;1(12):86-94.
doi:10.47278/book.ahp/2022.12 .

Štrbac, Filip, Bosco, Antonio, Pušić, Ivan, Stojanović, Dragica, Simin, Nataša, Cringoli, Giuseppe, Rinaldi, Laura, Ratajac, Radomir, "The Use of Essential Oils against Sheep Gastrointestinal Nematodes" in Animal Health Perspectives, 1, no. 12 (2022):86-94,
https://doi.org/10.47278/book.ahp/2022.12 . .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1972
AB  - Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.
PB  - World Association for the Advancement of Veterinary Parasitology (WAAVP)
C3  - Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
T1  - In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes
EP  - 517
SP  - 517
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1972
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Economic losses caused by sheep gastrointestinal nematodes (GIN) are increasing nowadays due to the development of anthelmintic resistance, therefore requiring a search for alternatives. In doing so, field testing of new potential substances provides credible and realistic insight of current possibility of their use. The aim of this study was to evaluate the in vivo anthelmintic potential of thyme, Thymus vulgaris L. essential oil (EO) and synergistic combination of two compounds, linalool:estragole on days 7 and 14 post-treatment using the faecal egg count reduction test (FECRT). The experiment was conducted on two separate farms located in southern Italy, whereby the total number of sheep (n=96, n=48 per farm) was divided into four groups on each farm (n=12). Groups received the following treatments perorally: G1 – 100 mg kg-1 T. vulgaris; G2 – 100 mg kg-1 linalool:estragole; G3 – standard dose of fenbendazole and G4 – 50 ml per animal of sunflower oil. Main compounds of thyme EO identified by gas chromatographymass spectrometry (GC-MS) analyses were p-cymene (41.72%), thymol (31.59%) and α-terpineol (11.71%), and the coproculture examination showed the presence of four genera of sheep GIN on tested farms: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. In total, results of the FECRT showed
the following reductions of GIN eggs on day 7: 24.95% (T. vulgaris); 24.64% (linalool:estragole) and 82.49% (fenbendazole). On day 14, reductions of GIN eggs were: 23.72% (T. vulgaris), 25.91% (linalool:estragole) and 88.82% (fenbendazole). Furthermore, no toxic effects were observed in animals at these doses. The present study reveals the anthelmintic potential of tested substances. However, a new trial with modified conditions is needed to improve their effectiveness: increased dosage, a different way of administration or possible use of encapsulated or nanoencapsulated forms of these substances, especially if bearing in the mind anatomical-physiological specifics of the ruminant gastrointestinal tract.",
publisher = "World Association for the Advancement of Veterinary Parasitology (WAAVP)",
journal = "Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland",
title = "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes",
pages = "517-517",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1972"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland
World Association for the Advancement of Veterinary Parasitology (WAAVP)., 517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes. in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland. 2021;:517-517.
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vivo Potential of Thyme (Thymus vulgaris L.) Essential Oil and Synergistic Combination of Linalool:Estragole to Control Sheep Gastrointestinal Nematodes" in Book of Abstracts of the 28th International Conference of the World Association for the Advancement of Veterinary Parasitology (WAAVP), Dublin, Ireland (2021):517-517,
https://hdl.handle.net/21.15107/rcub_rimsi_1972 .

TY  - CONF
AU  - Simin, Stanislav
AU  - Živković, Nebojša
AU  - Pušić, Ivan
AU  - Stojanov, Igor
AU  - BECSKEI, Zsolt
AU  - Lalošević, Vesna
AU  - Vračar, Vuk
AU  - Štrbac, Filip
AU  - Ratajac, Radomir
AU  - Rinaldi, Laura
AU  - Sotiraki, Smaragda
AU  - Charlier, Johannes
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1973
AB  - Heavy reliance on anthelmintics to control gastrointestinal nematodes (GIN) of grazing ruminants, led to the emergence of anthelmintic resistance (AR), a well known global problem to sustainable animal production, health and welfare. Ruminant producers in Serbia are seldomly aware of the serious losses that GIN can cause. Although resistance of Trichostrongylus spp. to ivermectin (IVM) was detected earlier in sheep, they rarely know about the existence of AR. To address the AR issue in a new manner, several Serbian researchers attended COMBAR training schools (TSs) and short term scientific missions (STSMs) in order to acquire new skills for improved diagnostics and control of GIN, such as the application of the Mini FLOTAC technique and the conduct of faecal egg count reduction tests (FECRTs) for monitoring anthelmintic efficacy. Using Mini FLOTAC, a set of small scale surveys was performed, to monitor GIN in grazing cattle (50 animals from 5 herds) and assess anthelmintic efficacy in sheep (11 farms tested for IVM, 3 farms tested for levamisole (LEV)) and goats (one farm tested for IVM, eprinomectin (EPR) and albendazole (ALB)). Results showed low levels of GIN infection in cattle (average 13 eggs per gram (epg), range 5-95 epg). In the goat farm, resistance to EPR and IVM was detected (percentage of egg reductions= 83 and 92%, respectively), while ALB retained full efficacy. Regarding sheep, AR to IVM was established in 8 farms (73%), with egg reductions ranging from 55 to 92%, while LEV showed full efficacy against GIN. An STSM supported the evaluation of essential oils from Serbian native plants against GIN using in vitro studies and showed promising results. Overall, COST Action COMBAR is contributing to sustainable parasite control in Serbia through training researchers in new research practices.
PB  - Serbian Society of Parasitology
C3  - Proceedings of 3th European Multicolloquium of Parasitology: „Changing climate changing parasites“
T1  - Combating Anthelmintic Resistance in Ruminants: A Serbian Perspective
EP  - 55
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1973
ER  - 

@conference{
author = "Simin, Stanislav and Živković, Nebojša and Pušić, Ivan and Stojanov, Igor and BECSKEI, Zsolt and Lalošević, Vesna and Vračar, Vuk and Štrbac, Filip and Ratajac, Radomir and Rinaldi, Laura and Sotiraki, Smaragda and Charlier, Johannes",
year = "2021",
abstract = "Heavy reliance on anthelmintics to control gastrointestinal nematodes (GIN) of grazing ruminants, led to the emergence of anthelmintic resistance (AR), a well known global problem to sustainable animal production, health and welfare. Ruminant producers in Serbia are seldomly aware of the serious losses that GIN can cause. Although resistance of Trichostrongylus spp. to ivermectin (IVM) was detected earlier in sheep, they rarely know about the existence of AR. To address the AR issue in a new manner, several Serbian researchers attended COMBAR training schools (TSs) and short term scientific missions (STSMs) in order to acquire new skills for improved diagnostics and control of GIN, such as the application of the Mini FLOTAC technique and the conduct of faecal egg count reduction tests (FECRTs) for monitoring anthelmintic efficacy. Using Mini FLOTAC, a set of small scale surveys was performed, to monitor GIN in grazing cattle (50 animals from 5 herds) and assess anthelmintic efficacy in sheep (11 farms tested for IVM, 3 farms tested for levamisole (LEV)) and goats (one farm tested for IVM, eprinomectin (EPR) and albendazole (ALB)). Results showed low levels of GIN infection in cattle (average 13 eggs per gram (epg), range 5-95 epg). In the goat farm, resistance to EPR and IVM was detected (percentage of egg reductions= 83 and 92%, respectively), while ALB retained full efficacy. Regarding sheep, AR to IVM was established in 8 farms (73%), with egg reductions ranging from 55 to 92%, while LEV showed full efficacy against GIN. An STSM supported the evaluation of essential oils from Serbian native plants against GIN using in vitro studies and showed promising results. Overall, COST Action COMBAR is contributing to sustainable parasite control in Serbia through training researchers in new research practices.",
publisher = "Serbian Society of Parasitology",
journal = "Proceedings of 3th European Multicolloquium of Parasitology: „Changing climate changing parasites“",
title = "Combating Anthelmintic Resistance in Ruminants: A Serbian Perspective",
pages = "55-55",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1973"
}

Simin, S., Živković, N., Pušić, I., Stojanov, I., BECSKEI, Z., Lalošević, V., Vračar, V., Štrbac, F., Ratajac, R., Rinaldi, L., Sotiraki, S.,& Charlier, J.. (2021). Combating Anthelmintic Resistance in Ruminants: A Serbian Perspective. in Proceedings of 3th European Multicolloquium of Parasitology: „Changing climate changing parasites“
Serbian Society of Parasitology., 55-55.
https://hdl.handle.net/21.15107/rcub_rimsi_1973

Simin S, Živković N, Pušić I, Stojanov I, BECSKEI Z, Lalošević V, Vračar V, Štrbac F, Ratajac R, Rinaldi L, Sotiraki S, Charlier J. Combating Anthelmintic Resistance in Ruminants: A Serbian Perspective. in Proceedings of 3th European Multicolloquium of Parasitology: „Changing climate changing parasites“. 2021;:55-55.
https://hdl.handle.net/21.15107/rcub_rimsi_1973 .

Simin, Stanislav, Živković, Nebojša, Pušić, Ivan, Stojanov, Igor, BECSKEI, Zsolt, Lalošević, Vesna, Vračar, Vuk, Štrbac, Filip, Ratajac, Radomir, Rinaldi, Laura, Sotiraki, Smaragda, Charlier, Johannes, "Combating Anthelmintic Resistance in Ruminants: A Serbian Perspective" in Proceedings of 3th European Multicolloquium of Parasitology: „Changing climate changing parasites“ (2021):55-55,
https://hdl.handle.net/21.15107/rcub_rimsi_1973 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1975
AB  - Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.
PB  - Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
T1  - Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties
EP  - 161
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1975
ER  - 

@conference{
author = "Štrbac, Filip and Simin, Nataša and Orčić, Dejan and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Thyme (Thymus vulgaris L.) is a well-known member of the genus Thymus with healing properties known since ancient times. Thyme has application in traditional medicine and pharmaceutical preparations, but also in the food and cosmetic industry. The aim of this study was to examine the chemical composition and in vitro anthelmintic potential of thyme essential oils from Serbia and compare it with sample from France. Chemical characterization of the samples was done by GC-MS and antihelmintic potential of tested samples was evaluated using in vitro egg hatch test on sheep gastrointestinal nematodes (concentration range 0.049-50 mg/mL) for each sample. The most represented compounds in sample from Serbia were: p-cymene (41.72%), thymol (31.59%), α-terpineol (11.71%), linalool (4.37%) and α-pinene (2.47%) and it inhibited egg hatchability for 95.25-100% and in sample from France thymol (54.48%), p-cymene (21.01%), γ-terpinene (8.11%), carvacrol (3.95%) and linalool (2.77%) were the dominant compounds and inhibited egg
hatchability for 98.5-100%. The results indicate that chemical composition of thyme essential oil is dependant on geographic origin but regardless it poseses antihelmintic potential and may be used in veterinary medicine as anthelmintic agent.",
publisher = "Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"",
title = "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties",
pages = "161-160",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1975"
}

Štrbac, F., Simin, N., Orčić, D., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms"
Faculty of Chemistry, University of Belgrade; Serbian Biochemical Society., 160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975

Štrbac F, Simin N, Orčić D, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Pušić I, Krnjajić S, Ratajac R. Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties. in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms". 2021;:160-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

Štrbac, Filip, Simin, Nataša, Orčić, Dejan, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Chemical Composition of Thyme Essential Oil and its Anthelmintic Properties" in Serbian Biochemical Society Tenth Conference with international participation - "Biochemical Insights into Molecular Mechanisms" (2021):160-161,
https://hdl.handle.net/21.15107/rcub_rimsi_1975 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Mangieri, Giuseppe
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1888
AB  - Based on the results in different studies conducted so far, plant essential oils (EOs) are recently been marked as a possible solution for the problem of anthelmintic resistance (AR). However, their efficacy against a wide range of livestock parasites is due to active ingredients. In that context, the aim of this study was to evaluate the in vitro ovicidal effect of a mixture of linalool and estragole (14% : 86%, respectively) against sheep gastrointestinal nematodes (GINs) using the egg hatch test (EHT). The study was conducted using faecal samples of naturally infected sheep by GINs from two different farms located in Southern Italy. Coproculture of tested faecal samples was performed, as well as chemical analyses of the tested mixture. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) and the obtained values were compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (3% Tween 80, v/v). The tested binary combination showed high and dose-dependent ovicidal activity varied from 29.5% to 100%. The inhibitory effect on egg hatchability was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (100%), 12.5 mg/mL (100%) and 3.125 (99.8%). Moreover, all tested concentrations showed significantly higher (p<0.001) activity compared to the negative control. The obtained results suggest that the combination of linalool:estragole has the potential to be used against sheep GINs. These also highlight the possible role of different combinations of certain constituents of essential oils in combating AR. However, these findings need confirmation in further in vivo studies.
AB  - Na osnovu rezultata do sada sprovedenih istraživanja, etarska ulja biljaka su označena kao jedna od mogućih opcija za rešenje problemarezistencije na antihelmintike. Pri tome, za njihovu efikasnost protiv različitih parazita domaćih životinja odgovorni su njihovi aktivni sastojci. U tom kontekstu, cilj ovog istraživanja je bio ispitati ovicidnu efikasnost smeše linaloola i estragola (14% i 86%, tim redom) protiv gastrointestinalnih nematoda (GIN) ovaca koristeći test izleganja jaja (egg hatch test (EHT)). U studiji su korišćeni uzorci fecesa ovaca prirodno inficiranih GIN sa dve različite farme u južnoj Italiji. Koprokultura testiranih uzoraka je sprovedena, kao i hemijske analize testirane smeše. EHT je sproveden za šest različitih koncentracija (50; 12,5; 3,125; 0,781; 0,195 i 0,049 mg/mL), pri čemu su dobijeni rezultati upoređeni sa pozitivnom (tiabendazol, 0.025 mg/mL) i negativnom (3% Tween 80) kontrolom. Testirana binarna kombinacija je pokazala snažnu, dozno-zavisnu ovicidnu aktivnost koja je varirala između 29,5% i 100%. Inhibitorni efekat na izleganje larvi je bio sličan (p<0,05) pozitivnoj kontroli (98,0%) pri koncentracijama od 50 mg/mL (100%), 12,5 mg/mL (100%) i 3,125 mg/mL (99,8%). Pri tome, kod svih testiranih koncentracija efekat je bio značajno veći (p<0,001) u poređenju sa negativnom kontrolom. Dobijeni rezultati ukazuju na to da ispitana smeša linaloola i estragola ima potencijal da se koristi protiv GIN ovaca. Takođe, ova studija ukazuje na moguću ulogu različitih sastojaka biljnih etarskih ulja u borbi protiv rezistencije na antihelmintike. Međutim, ovi nalazi zahtevaju dodatnu potvrdu u budućim in vivo ispitivanjima.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep
T1  - In vitro ovicidna aktivnost smeše linaloola i estragola protiv gastrointestinalnih nematoda ovaca
EP  - 59
IS  - 1
SP  - 49
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1888
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Mangieri, Giuseppe and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2021",
abstract = "Based on the results in different studies conducted so far, plant essential oils (EOs) are recently been marked as a possible solution for the problem of anthelmintic resistance (AR). However, their efficacy against a wide range of livestock parasites is due to active ingredients. In that context, the aim of this study was to evaluate the in vitro ovicidal effect of a mixture of linalool and estragole (14% : 86%, respectively) against sheep gastrointestinal nematodes (GINs) using the egg hatch test (EHT). The study was conducted using faecal samples of naturally infected sheep by GINs from two different farms located in Southern Italy. Coproculture of tested faecal samples was performed, as well as chemical analyses of the tested mixture. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) and the obtained values were compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (3% Tween 80, v/v). The tested binary combination showed high and dose-dependent ovicidal activity varied from 29.5% to 100%. The inhibitory effect on egg hatchability was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (100%), 12.5 mg/mL (100%) and 3.125 (99.8%). Moreover, all tested concentrations showed significantly higher (p<0.001) activity compared to the negative control. The obtained results suggest that the combination of linalool:estragole has the potential to be used against sheep GINs. These also highlight the possible role of different combinations of certain constituents of essential oils in combating AR. However, these findings need confirmation in further in vivo studies., Na osnovu rezultata do sada sprovedenih istraživanja, etarska ulja biljaka su označena kao jedna od mogućih opcija za rešenje problemarezistencije na antihelmintike. Pri tome, za njihovu efikasnost protiv različitih parazita domaćih životinja odgovorni su njihovi aktivni sastojci. U tom kontekstu, cilj ovog istraživanja je bio ispitati ovicidnu efikasnost smeše linaloola i estragola (14% i 86%, tim redom) protiv gastrointestinalnih nematoda (GIN) ovaca koristeći test izleganja jaja (egg hatch test (EHT)). U studiji su korišćeni uzorci fecesa ovaca prirodno inficiranih GIN sa dve različite farme u južnoj Italiji. Koprokultura testiranih uzoraka je sprovedena, kao i hemijske analize testirane smeše. EHT je sproveden za šest različitih koncentracija (50; 12,5; 3,125; 0,781; 0,195 i 0,049 mg/mL), pri čemu su dobijeni rezultati upoređeni sa pozitivnom (tiabendazol, 0.025 mg/mL) i negativnom (3% Tween 80) kontrolom. Testirana binarna kombinacija je pokazala snažnu, dozno-zavisnu ovicidnu aktivnost koja je varirala između 29,5% i 100%. Inhibitorni efekat na izleganje larvi je bio sličan (p<0,05) pozitivnoj kontroli (98,0%) pri koncentracijama od 50 mg/mL (100%), 12,5 mg/mL (100%) i 3,125 mg/mL (99,8%). Pri tome, kod svih testiranih koncentracija efekat je bio značajno veći (p<0,001) u poređenju sa negativnom kontrolom. Dobijeni rezultati ukazuju na to da ispitana smeša linaloola i estragola ima potencijal da se koristi protiv GIN ovaca. Takođe, ova studija ukazuje na moguću ulogu različitih sastojaka biljnih etarskih ulja u borbi protiv rezistencije na antihelmintike. Međutim, ovi nalazi zahtevaju dodatnu potvrdu u budućim in vivo ispitivanjima.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep, In vitro ovicidna aktivnost smeše linaloola i estragola protiv gastrointestinalnih nematoda ovaca",
pages = "59-49",
number = "1",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1888"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Mangieri, G., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2021). In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 2(1), 49-59.
https://hdl.handle.net/21.15107/rcub_rimsi_1888

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Mangieri G, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep. in Veterinarski Pregled. 2021;2(1):49-59.
https://hdl.handle.net/21.15107/rcub_rimsi_1888 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Mangieri, Giuseppe, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vitro Ovicidal Activity of Mixture of Linalool and Estragole against Gastrointestinal Nematodes of Sheep" in Veterinarski Pregled, 2, no. 1 (2021):49-59,
https://hdl.handle.net/21.15107/rcub_rimsi_1888 .

TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Maurelli, Maria Paola
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1974
AB  - The worldwide increased difficulty to contrast gastrointestinal nematode (GIN) infections in sheep, due to growing anthelmintic resistance, has led to find alternative helminth control strategies. This study aimed to evaluate the in vitro ovicidal activity of essential oils (EOs) of Juniperus communis and Achillea millefolium (two chemotypes) against GINs of sheep. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in Southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO and compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (Tween 80 3%, v/v). In both farms, coprocultures revealed the presence of four genera of GINs: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The inhibitory effect on GIN eggs’ hatchability varied from 81% to 96.75% for J. communis EO. When using A. millefolium EO, the ovicidal activity against GIN was 46.5-99.5% (type 1) and 69.6-97.25% (type 2). Furthermore, the effect of tested EOs was very high at concentrations of 50 mg/mL (96.75%), 12.5 mg/mL (95.5%) and 3.125 mg/mL (94.75%) of J. communis EO, whilst for A. millefolium EO at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) (type 1), and 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) (type 2), showing a similar effect (p>0.05) to the positive control (98%). Therefore, the findings of this study showed the anthelmintic potential of the tested EOs and highlight the importance of ethnopharmacology to control GIN infection in sheep.
PB  - COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230
C3  - 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”
T1  - Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep
EP  - 26
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1974
ER  - 

@conference{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Maurelli, Maria Paola and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "The worldwide increased difficulty to contrast gastrointestinal nematode (GIN) infections in sheep, due to growing anthelmintic resistance, has led to find alternative helminth control strategies. This study aimed to evaluate the in vitro ovicidal activity of essential oils (EOs) of Juniperus communis and Achillea millefolium (two chemotypes) against GINs of sheep. For this purpose, the nematode eggs were collected from naturally infected sheep by GINs in two farms located in Southern Italy. The egg hatch test (EHT) was performed at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL) for each EO and compared to the positive control (thiabendazole, 0.025 mg/mL) and the negative control (Tween 80 3%, v/v). In both farms, coprocultures revealed the presence of four genera of GINs: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The inhibitory effect on GIN eggs’ hatchability varied from 81% to 96.75% for J. communis EO. When using A. millefolium EO, the ovicidal activity against GIN was 46.5-99.5% (type 1) and 69.6-97.25% (type 2). Furthermore, the effect of tested EOs was very high at concentrations of 50 mg/mL (96.75%), 12.5 mg/mL (95.5%) and 3.125 mg/mL (94.75%) of J. communis EO, whilst for A. millefolium EO at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) (type 1), and 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) (type 2), showing a similar effect (p>0.05) to the positive control (98%). Therefore, the findings of this study showed the anthelmintic potential of the tested EOs and highlight the importance of ethnopharmacology to control GIN infection in sheep.",
publisher = "COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230",
journal = "4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”",
title = "Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep",
pages = "26-26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1974"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Maurelli, M. P., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2020). Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep. in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”
COST Action COMBAR (Combatting Anthelmintic Resistance in Ruminants) - CA16230., 26-26.
https://hdl.handle.net/21.15107/rcub_rimsi_1974

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Maurelli MP, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep. in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations”. 2020;:26-26.
https://hdl.handle.net/21.15107/rcub_rimsi_1974 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "Ovicidal Potential of Essential Oils of Juniperus communis and Achillea millefolium to Control Gastrointestinal Nematodes in Sheep" in 4th Joint COMBAR WG meetings: “Anthelmintic Resistance in Ruminants: from Research to Recommendations” (2020):26-26,
https://hdl.handle.net/21.15107/rcub_rimsi_1974 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1885
AB  - The negative influence of gastrointestinal parasitism in sheep is growing today due to the development of anthelmintic resistance to commercial drugs. For this reason, researchers around the world are looking for new, alternative strategies for controlling these parasites. In this context, medicinal plants and their products are increasingly mentioned. The aim of this study was to evaluate in vitro ovicidal activity of common juniper (Juniperus communis L.) essential oil against sheep gastrointestinal nematodes. For that purpose, the egg hatch test was used, and the obtained results were analyzed by analysis of variance (ANOVA) followed by comparison with Tukey’s test (p<0.05). Faecal samples were collected from two independent, natural-infected farms located in Eboli (SA), Italy, whereby the coproculture examination identified the presence of four genera of sheep gastrointestinal nematodes: Haemonchus (53%), Trichostrngylus (29.5%), Teladorsagia (14.5%) and Chabertia (3%). Main components of common juniper essential oil identified by GC-MS analysis were α-pinene (40.46%), sabinene (14.04%), myrcene (8.87%) and limonene (4.95%). In vitro ovicidal activity was evaluated at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby J. communis essential oil showed high activity with the inhibitory effect on hatchability of 81-96.75%, depending on the concentration. For all concentration tested, the effect was statistically significantly higher compared to the negative control, while for the three highest concentrations the effect was similar to the positive control. The obtained results suggested that J. communis essential oil have high anthelmintic potential. However, these results need confirmation in further field examination.
AB  - Negativni uticaj gastrointestinalnih parazita kod ovaca u današnje vreme raste usled razvoja antihelmintičke rezistencije na komercijalne preparate. Iz tog razloga, istraživači širom sveta tragaju za novim, alternativnim strategijama u cilju kontrole ovih parazita. U tom kontekstu, lekovite biljke i njihovi proizvodi se sve češće spominju. Cilj ovog istraživanja je bio ispitati in vitro ovicidnu aktivnost esencijalnog ulja kleke (Juniperus communis L.) protiv gastrointestinalnih nematoda ovaca. Za ovu svrhu je upotrebljen egg hatch test, nakon čega su dobijeni rezultati obrađeni analizom varijanse i potom upoređeni Tukijevim testom (p<0.05). Uzorci fecesa su uzeti sa dve odvojene farme koje se nalaze u oblasti Eboli (SA), Italija, pri čemu je tokom ispitivanja koprokulture identifikovano prisustvo četiri roda gastrointestinalnih nematoda ovaca: Haemonchus (53%), Trichostrongylus (29.5%), Teladorsagia (14.5%) i Chaberita (3%). Najzastupljenije komponente etarskog ulja kleke identifikovane biohemijskim analizama su bile α-pinen (40.46%), sabinen (14.04%), mircen (8.87%) i limonen (4.95%*). In vitro ovicidna aktivnost je testirana za šest različitih koncentracija (50, 12.5, 3.125, 0.781, 0.195 i 0.049 mg/mL), pri čemu je etarsko ulje kleke pokazalo visoku aktivnost sa inhibitornim efektom na izleganje larvi od 81-96.75%, u zavisnosti od koncentracije. Kod svih ispitivanih koncentracija efekat je bio statistički značajno veći u poređenju sa negativnom kontrolom, dok je kod tri najveće koncentracije efekat bio sličan pozitivnoj kontroli. Dobijeni rezultati ukazuju na to da etarsko ulje kleke ima visok antihelmintički potencijal. Međutim, potrebno je ove rezultate potvrditi u budućim ispitivanjima u terenskim uslovima.
PB  - Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu
T2  - Veterinarski Pregled
T1  - In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes
T1  - In vitro ovicidan efekat etarskog ulja kleke (Juniperus communis L.) na gastrointestinalne nematode ovaca
EP  - 159
IS  - 1
SP  - 152
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1885
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "The negative influence of gastrointestinal parasitism in sheep is growing today due to the development of anthelmintic resistance to commercial drugs. For this reason, researchers around the world are looking for new, alternative strategies for controlling these parasites. In this context, medicinal plants and their products are increasingly mentioned. The aim of this study was to evaluate in vitro ovicidal activity of common juniper (Juniperus communis L.) essential oil against sheep gastrointestinal nematodes. For that purpose, the egg hatch test was used, and the obtained results were analyzed by analysis of variance (ANOVA) followed by comparison with Tukey’s test (p<0.05). Faecal samples were collected from two independent, natural-infected farms located in Eboli (SA), Italy, whereby the coproculture examination identified the presence of four genera of sheep gastrointestinal nematodes: Haemonchus (53%), Trichostrngylus (29.5%), Teladorsagia (14.5%) and Chabertia (3%). Main components of common juniper essential oil identified by GC-MS analysis were α-pinene (40.46%), sabinene (14.04%), myrcene (8.87%) and limonene (4.95%). In vitro ovicidal activity was evaluated at six different concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby J. communis essential oil showed high activity with the inhibitory effect on hatchability of 81-96.75%, depending on the concentration. For all concentration tested, the effect was statistically significantly higher compared to the negative control, while for the three highest concentrations the effect was similar to the positive control. The obtained results suggested that J. communis essential oil have high anthelmintic potential. However, these results need confirmation in further field examination., Negativni uticaj gastrointestinalnih parazita kod ovaca u današnje vreme raste usled razvoja antihelmintičke rezistencije na komercijalne preparate. Iz tog razloga, istraživači širom sveta tragaju za novim, alternativnim strategijama u cilju kontrole ovih parazita. U tom kontekstu, lekovite biljke i njihovi proizvodi se sve češće spominju. Cilj ovog istraživanja je bio ispitati in vitro ovicidnu aktivnost esencijalnog ulja kleke (Juniperus communis L.) protiv gastrointestinalnih nematoda ovaca. Za ovu svrhu je upotrebljen egg hatch test, nakon čega su dobijeni rezultati obrađeni analizom varijanse i potom upoređeni Tukijevim testom (p<0.05). Uzorci fecesa su uzeti sa dve odvojene farme koje se nalaze u oblasti Eboli (SA), Italija, pri čemu je tokom ispitivanja koprokulture identifikovano prisustvo četiri roda gastrointestinalnih nematoda ovaca: Haemonchus (53%), Trichostrongylus (29.5%), Teladorsagia (14.5%) i Chaberita (3%). Najzastupljenije komponente etarskog ulja kleke identifikovane biohemijskim analizama su bile α-pinen (40.46%), sabinen (14.04%), mircen (8.87%) i limonen (4.95%*). In vitro ovicidna aktivnost je testirana za šest različitih koncentracija (50, 12.5, 3.125, 0.781, 0.195 i 0.049 mg/mL), pri čemu je etarsko ulje kleke pokazalo visoku aktivnost sa inhibitornim efektom na izleganje larvi od 81-96.75%, u zavisnosti od koncentracije. Kod svih ispitivanih koncentracija efekat je bio statistički značajno veći u poređenju sa negativnom kontrolom, dok je kod tri najveće koncentracije efekat bio sličan pozitivnoj kontroli. Dobijeni rezultati ukazuju na to da etarsko ulje kleke ima visok antihelmintički potencijal. Međutim, potrebno je ove rezultate potvrditi u budućim ispitivanjima u terenskim uslovima.",
publisher = "Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu",
journal = "Veterinarski Pregled",
title = "In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes, In vitro ovicidan efekat etarskog ulja kleke (Juniperus communis L.) na gastrointestinalne nematode ovaca",
pages = "159-152",
number = "1",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1885"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2020). In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes. in Veterinarski Pregled
Departman za veterinarsku medicinu, Poljoprivredni fakultet, Univerzitet u Novom Sadu., 1(1), 152-159.
https://hdl.handle.net/21.15107/rcub_rimsi_1885

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes. in Veterinarski Pregled. 2020;1(1):152-159.
https://hdl.handle.net/21.15107/rcub_rimsi_1885 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vitro Ovicidal Effect of Common Juniper (Juniperus communis L.) Essential Oil on Sheep Gastrointestinal Nematodes" in Veterinarski Pregled, 1, no. 1 (2020):152-159,
https://hdl.handle.net/21.15107/rcub_rimsi_1885 .

TY  - JOUR
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Amadesi, Alessandra
AU  - Rinaldi, Laura
AU  - Stojanović, Dragica
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Pušić, Ivan
AU  - Krnjajić, Slobodan
AU  - Ratajac, Radomir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1882
AB  - Economic losses caused by the gastrointestinal nematodes of small ruminants have been on increase mainly due to the development of anthelmintic resistance. Therefore, researchers from  all around the world are searching for new, alternative strategies to control these parasites. Being a valuable natural resource, medicinal plants and their products have emerged as a viable option. This study aimed to evaluate the in vitro ovicidal activity of two chemotypes of yarrow (Achillea millefolium L.) essential oil against ovine gastrointestinal nematode eggs. Nematode eggs obtained from the faeces of naturally infected sheep on two farms located in Southern Italy were subjected to the egg hatch test. On both farms, the coproculture examination identified the presence of species belonging to four genera of sheep gastrointestinal nematodes: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The main components of the yarrow essential oil identified by GC-MS analysis were 1,8-cineole (41.69%), camphor (8.37%) and trans-chrysanthenyl acetate (4.90%) in the oil type 1 and β-pinene (28.53%), β-caryophyllene (18.71%) and 1,8-cineole (11.69%) in type 2. The in vitro ovicidal activity was evaluated at six oil concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby the inhibitory effect of the essential oil on egg hatchability varied from 46.5-99.5% (type 1) and from 69.6-97.25% (type 2). All concentrations tested showed a significantly higher efficacy compared to the negative control (p<0.0001). The inhibitory effect on egg hatching was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) of the oil type 1, and at concentrations of 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) of the type 2. The obtained results suggested that the A. millefolium essential oil has high anthelmintic potential, especially the chemotype rich in 1,8-cineole and camphor, which requires confirmation in further in vivo studies.
AB  - Ekonomski gubici koje prouzrokuju gastrointestinalne nematode malih preživara u poslednje vreme rastu usled razvoja rezistencije na antihelmintike. Zbog toga istraživači širom sveta tragaju za novim, alternativnim strategijama za kontrolu ovih parazita. Kao dragocen prirodan resurs, lekovite biljke i njihovi proizvodi nametnuli su se kao moguća opcija. Cilj ovog istraživanja je bio utvrditi in vitro ovicidnu aktivnost dva hemotipa etarskog ulja hajdučke trave (Achillea millefolium L.) protiv jaja gastrointestinalnih nematoda ovaca. Jaja nematoda su izolovana iz fecesa prirodno inficiranih ovaca sa dve farme locirane u južnoj Italiji kako bi se sproveo test izleganja larvi (eng. egg hatch test). Na obe farme, koprokulturološkim ispitivanjima je utvrđeno prisustvo vrsta četiri roda gastrointestinalnih nematoda ovaca: Haemonchus, Trichostrongylus, Teladorsagia i Chabertia. Najzastupljenije komponente  etarskog ulja hajdučke trave identifikovane GC-MS analizama bile su 1,8-cineol (41,69%), kamfor (8,37%)  i  trans-hrizantenil acetat (4,90%) u ulju tipa 1, odnosno β-pinen (28,53%), β-kariofilen (18,71%) i 1,8-cineol (11,69%) u tipu 2. In vitro ovicidna aktivnost je testirana u šest različitih koncentracija (50; 12,5;  3,125; 0,781; 0,195 i 0,049 mg/mL). Inhibitorni efekat etarskog ulja na izleganje jaja je varirao od 46,5-99,5% (ulje tipa 1), odnosno 69,6-97,25% (tip 2), dok je kod svih ispitivanih koncentracija efekat bio značajno veći u poređenju sa negativnom kontrolom (p<0.0001). Pored toga, inhibitorni efekat na izgleganje larvi je bio sličan (p>0.05) pozitivnoj kontroli (98,0%) pri koncentracijama 50 mg/mL (99,5%), 12,5 mg/mL (98,0%) i 3,125 mg/mL (95,25%) ulja tipa 1, odnosno pri koncentracijama od 50 mg/mL (97,25%) i 12,5 mg/mL (90,0%) kod tipa 2. Dobijeni rezultati ukazuju na to da etarsko ulje hajdučke trave, naročito tipa 1 koje je bogato 1,8-cineolom i kamforom, poseduje visok antihelmintički potencijal, što zahteva potvrdu u daljim in vivo ispitivanjima.
PB  - Naučni institut za veterinarstvo Novi Sad
T2  - Arhiv Veterinarske Medicine
T1  - In Vitro Ovicidal Activity of Two Chemotypes of the Yarrow (Achillea millefolium L.) Essential Oil against Ovine Gastrointestinal Nematode Eggs
T1  - In vitro ovicidna aktivnost dva tipa etarskog ulja hajdučke trave (Achillea millefolium L.) protiv jaja gastrointestinalnih nematoda ovaca
EP  - 76
IS  - 2
SP  - 59
VL  - 13
DO  - 10.46784/eavm.v13i2.246
ER  - 

@article{
author = "Štrbac, Filip and Bosco, Antonio and Amadesi, Alessandra and Rinaldi, Laura and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Pušić, Ivan and Krnjajić, Slobodan and Ratajac, Radomir",
year = "2020",
abstract = "Economic losses caused by the gastrointestinal nematodes of small ruminants have been on increase mainly due to the development of anthelmintic resistance. Therefore, researchers from  all around the world are searching for new, alternative strategies to control these parasites. Being a valuable natural resource, medicinal plants and their products have emerged as a viable option. This study aimed to evaluate the in vitro ovicidal activity of two chemotypes of yarrow (Achillea millefolium L.) essential oil against ovine gastrointestinal nematode eggs. Nematode eggs obtained from the faeces of naturally infected sheep on two farms located in Southern Italy were subjected to the egg hatch test. On both farms, the coproculture examination identified the presence of species belonging to four genera of sheep gastrointestinal nematodes: Haemonchus, Trichostrongylus, Teladorsagia and Chabertia. The main components of the yarrow essential oil identified by GC-MS analysis were 1,8-cineole (41.69%), camphor (8.37%) and trans-chrysanthenyl acetate (4.90%) in the oil type 1 and β-pinene (28.53%), β-caryophyllene (18.71%) and 1,8-cineole (11.69%) in type 2. The in vitro ovicidal activity was evaluated at six oil concentrations (50, 12.5, 3.125, 0.781, 0.195 and 0.049 mg/mL), whereby the inhibitory effect of the essential oil on egg hatchability varied from 46.5-99.5% (type 1) and from 69.6-97.25% (type 2). All concentrations tested showed a significantly higher efficacy compared to the negative control (p<0.0001). The inhibitory effect on egg hatching was similar (p>0.05) to the positive control (98.0%) at concentrations of 50 mg/mL (99.5%), 12.5 mg/mL (98.0%) and 3.125 (95.25%) of the oil type 1, and at concentrations of 50 mg/mL (97.25%) and 12.5 mg/mL (90.0%) of the type 2. The obtained results suggested that the A. millefolium essential oil has high anthelmintic potential, especially the chemotype rich in 1,8-cineole and camphor, which requires confirmation in further in vivo studies., Ekonomski gubici koje prouzrokuju gastrointestinalne nematode malih preživara u poslednje vreme rastu usled razvoja rezistencije na antihelmintike. Zbog toga istraživači širom sveta tragaju za novim, alternativnim strategijama za kontrolu ovih parazita. Kao dragocen prirodan resurs, lekovite biljke i njihovi proizvodi nametnuli su se kao moguća opcija. Cilj ovog istraživanja je bio utvrditi in vitro ovicidnu aktivnost dva hemotipa etarskog ulja hajdučke trave (Achillea millefolium L.) protiv jaja gastrointestinalnih nematoda ovaca. Jaja nematoda su izolovana iz fecesa prirodno inficiranih ovaca sa dve farme locirane u južnoj Italiji kako bi se sproveo test izleganja larvi (eng. egg hatch test). Na obe farme, koprokulturološkim ispitivanjima je utvrđeno prisustvo vrsta četiri roda gastrointestinalnih nematoda ovaca: Haemonchus, Trichostrongylus, Teladorsagia i Chabertia. Najzastupljenije komponente  etarskog ulja hajdučke trave identifikovane GC-MS analizama bile su 1,8-cineol (41,69%), kamfor (8,37%)  i  trans-hrizantenil acetat (4,90%) u ulju tipa 1, odnosno β-pinen (28,53%), β-kariofilen (18,71%) i 1,8-cineol (11,69%) u tipu 2. In vitro ovicidna aktivnost je testirana u šest različitih koncentracija (50; 12,5;  3,125; 0,781; 0,195 i 0,049 mg/mL). Inhibitorni efekat etarskog ulja na izleganje jaja je varirao od 46,5-99,5% (ulje tipa 1), odnosno 69,6-97,25% (tip 2), dok je kod svih ispitivanih koncentracija efekat bio značajno veći u poređenju sa negativnom kontrolom (p<0.0001). Pored toga, inhibitorni efekat na izgleganje larvi je bio sličan (p>0.05) pozitivnoj kontroli (98,0%) pri koncentracijama 50 mg/mL (99,5%), 12,5 mg/mL (98,0%) i 3,125 mg/mL (95,25%) ulja tipa 1, odnosno pri koncentracijama od 50 mg/mL (97,25%) i 12,5 mg/mL (90,0%) kod tipa 2. Dobijeni rezultati ukazuju na to da etarsko ulje hajdučke trave, naročito tipa 1 koje je bogato 1,8-cineolom i kamforom, poseduje visok antihelmintički potencijal, što zahteva potvrdu u daljim in vivo ispitivanjima.",
publisher = "Naučni institut za veterinarstvo Novi Sad",
journal = "Arhiv Veterinarske Medicine",
title = "In Vitro Ovicidal Activity of Two Chemotypes of the Yarrow (Achillea millefolium L.) Essential Oil against Ovine Gastrointestinal Nematode Eggs, In vitro ovicidna aktivnost dva tipa etarskog ulja hajdučke trave (Achillea millefolium L.) protiv jaja gastrointestinalnih nematoda ovaca",
pages = "76-59",
number = "2",
volume = "13",
doi = "10.46784/eavm.v13i2.246"
}

Štrbac, F., Bosco, A., Amadesi, A., Rinaldi, L., Stojanović, D., Simin, N., Orčić, D., Pušić, I., Krnjajić, S.,& Ratajac, R.. (2020). In Vitro Ovicidal Activity of Two Chemotypes of the Yarrow (Achillea millefolium L.) Essential Oil against Ovine Gastrointestinal Nematode Eggs. in Arhiv Veterinarske Medicine
Naučni institut za veterinarstvo Novi Sad., 13(2), 59-76.
https://doi.org/10.46784/eavm.v13i2.246

Štrbac F, Bosco A, Amadesi A, Rinaldi L, Stojanović D, Simin N, Orčić D, Pušić I, Krnjajić S, Ratajac R. In Vitro Ovicidal Activity of Two Chemotypes of the Yarrow (Achillea millefolium L.) Essential Oil against Ovine Gastrointestinal Nematode Eggs. in Arhiv Veterinarske Medicine. 2020;13(2):59-76.
doi:10.46784/eavm.v13i2.246 .

Štrbac, Filip, Bosco, Antonio, Amadesi, Alessandra, Rinaldi, Laura, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Pušić, Ivan, Krnjajić, Slobodan, Ratajac, Radomir, "In Vitro Ovicidal Activity of Two Chemotypes of the Yarrow (Achillea millefolium L.) Essential Oil against Ovine Gastrointestinal Nematode Eggs" in Arhiv Veterinarske Medicine, 13, no. 2 (2020):59-76,
https://doi.org/10.46784/eavm.v13i2.246 . .