TY  - CONF
AU  - Nakarada, Đura
AU  - Milosavljević, Dragica
AU  - Milivojević, Jasminka M
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1792
AB  - Strawberries are rich in various health-promoting compounds. A particularly high yield of these
molecules could be extracted using methanol which is unfortunately harmful to living beings. To
surpass the problem of solvent toxicity, in this paper, we have incorporated methanol extracts of
two novel strawberry cultivars into liposomes. Our results show that both extracts possess
significant antiradical activity towards hydroxyl and DPPH radicals, unaltered by lipids from
liposome membranes. These results indicate that otherwise toxic extracts could easily be turned into a promising pharmaceutical product.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings)
T1  - ANTIRADICAL ACTIVITY OF NEW STRAWBERRY CULTIVAR EXTRACTS INCORPORATED INTO LIPOSOMES – AN EPR STUDY
EP  - 560
SP  - 557
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1792
ER  - 

@conference{
author = "Nakarada, Đura and Milosavljević, Dragica and Milivojević, Jasminka M and Dragišić Maksimović, Jelena and Mojović, Miloš",
year = "2022",
abstract = "Strawberries are rich in various health-promoting compounds. A particularly high yield of these
molecules could be extracted using methanol which is unfortunately harmful to living beings. To
surpass the problem of solvent toxicity, in this paper, we have incorporated methanol extracts of
two novel strawberry cultivars into liposomes. Our results show that both extracts possess
significant antiradical activity towards hydroxyl and DPPH radicals, unaltered by lipids from
liposome membranes. These results indicate that otherwise toxic extracts could easily be turned into a promising pharmaceutical product.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings)",
title = "ANTIRADICAL ACTIVITY OF NEW STRAWBERRY CULTIVAR EXTRACTS INCORPORATED INTO LIPOSOMES – AN EPR STUDY",
pages = "560-557",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1792"
}

Nakarada, Đ., Milosavljević, D., Milivojević, J. M., Dragišić Maksimović, J.,& Mojović, M.. (2022). ANTIRADICAL ACTIVITY OF NEW STRAWBERRY CULTIVAR EXTRACTS INCORPORATED INTO LIPOSOMES – AN EPR STUDY. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 557-560.
https://hdl.handle.net/21.15107/rcub_rimsi_1792

Nakarada Đ, Milosavljević D, Milivojević JM, Dragišić Maksimović J, Mojović M. ANTIRADICAL ACTIVITY OF NEW STRAWBERRY CULTIVAR EXTRACTS INCORPORATED INTO LIPOSOMES – AN EPR STUDY. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings). 2022;:557-560.
https://hdl.handle.net/21.15107/rcub_rimsi_1792 .

Nakarada, Đura, Milosavljević, Dragica, Milivojević, Jasminka M, Dragišić Maksimović, Jelena, Mojović, Miloš, "ANTIRADICAL ACTIVITY OF NEW STRAWBERRY CULTIVAR EXTRACTS INCORPORATED INTO LIPOSOMES – AN EPR STUDY" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings) (2022):557-560,
https://hdl.handle.net/21.15107/rcub_rimsi_1792 .

TY  - JOUR
AU  - Milosavljević, Dragica
AU  - Maksimović, Vuk
AU  - Milivojević Jasminka
AU  - Nakarada Đura
AU  - Mojović, Miloš
AU  - Dragišić Maksimović, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1609
AB  - Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in ´Premy´ (1.53 mg eq GA g-1 FW), ´Sandra´ (30.60 mg eq Pg-3-g 100 g-1 FW) and ´Laetitia´ (56.32 mg 100 g-1 FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while ´Tea´, ´Premy´, and ´Joly´ were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in ´Garda´, ´Federica´, and ´Rumba´ (0.11, 0.08, and 0.06 U mg-1 prot, respectively). ´Laetitia´, ´Joly´, ´Arianna´, ´Tea´, and ´Mila´ cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers in terms of fruit as a functional food.
PB  - MDPI
T2  - Plants
T1  - Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars
IS  - 24
SP  - 3566
VL  - 11
DO  - 10.3390/plants11243566
ER  - 

@article{
author = "Milosavljević, Dragica and Maksimović, Vuk and Milivojević Jasminka and Nakarada Đura and Mojović, Miloš and Dragišić Maksimović, Jelena",
year = "2022",
abstract = "Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in ´Premy´ (1.53 mg eq GA g-1 FW), ´Sandra´ (30.60 mg eq Pg-3-g 100 g-1 FW) and ´Laetitia´ (56.32 mg 100 g-1 FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while ´Tea´, ´Premy´, and ´Joly´ were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in ´Garda´, ´Federica´, and ´Rumba´ (0.11, 0.08, and 0.06 U mg-1 prot, respectively). ´Laetitia´, ´Joly´, ´Arianna´, ´Tea´, and ´Mila´ cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers in terms of fruit as a functional food.",
publisher = "MDPI",
journal = "Plants",
title = "Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars",
number = "24",
pages = "3566",
volume = "11",
doi = "10.3390/plants11243566"
}

Milosavljević, D., Maksimović, V., Milivojević Jasminka, Nakarada Đura, Mojović, M.,& Dragišić Maksimović, J.. (2022). Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars. in Plants
MDPI., 11(24), 3566.
https://doi.org/10.3390/plants11243566

Milosavljević D, Maksimović V, Milivojević Jasminka, Nakarada Đura, Mojović M, Dragišić Maksimović J. Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars. in Plants. 2022;11(24):3566.
doi:10.3390/plants11243566 .

Milosavljević, Dragica, Maksimović, Vuk, Milivojević Jasminka, Nakarada Đura, Mojović, Miloš, Dragišić Maksimović, Jelena, "Rich in Phenolics—Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars" in Plants, 11, no. 24 (2022):3566,
https://doi.org/10.3390/plants11243566 . .

TY  - JOUR
AU  - Bartolić, Dragana
AU  - Mojović, Miloš
AU  - Prokopijević, Miloš
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Mutavdžić, Dragosav
AU  - Baosic, Rada
AU  - Radotić, Ksenija
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1544
AB  - BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.
PB  - Wiley, Hoboken
T2  - Journal of the Science of Food and Agriculture
T1  - Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study
EP  - 2505
IS  - 6
SP  - 2500
VL  - 102
DO  - 10.1002/jsfa.11591
ER  - 

@article{
author = "Bartolić, Dragana and Mojović, Miloš and Prokopijević, Miloš and Đikanović, Daniela and Kalauzi, Aleksandar and Mutavdžić, Dragosav and Baosic, Rada and Radotić, Ksenija",
year = "2022",
abstract = "BACKGROUND Aflatoxin B-1 (AFB(1)) is the most dangerous of the mycotoxins that contaminate cereal seeds naturally. A stress lignin formation is linked with the accumulation of reactive oxygen species causing a change in the redox status and formation of stable organic radicals, constituting the first layer of defense. The relationship between AFB(1) and changes in lignin organic free radicals in seeds is not known, nor is the part of the seed that is more targeted. Using optical and electron paramagnetic resonance spectroscopy, we investigated AFB(1)-induced changes in lignin and organic free radicals in seeds, and whether the inner and outer seed fractions differ in response to increasing AFB(1). RESULTS Different changes in the content of lignin and free radicals with increasing AFB(1) concentrations were observed in the two seed fractions. There was a significant positive linear correlation (R = 0.9923, P = 0.00005) between lignin content and AFB(1) concentration in the outer fraction, and no correlation between the lignin content and the AFB(1) concentration in the inner fraction. We found a positive correlation between the area of the green spectral emission component (C4) and the AFB(1) concentration in the outer fraction. CONCLUSIONS To the best of our knowledge, the results showed, for the first time, that maize seed fractions respond differently to aflatoxin with regard to their lignin and organic free radical content. Lignin content and (C4) area may be reliable indicators for the screening of lignin changes against AFB(1) content in the seeds, and thus for seed protection capacity.",
publisher = "Wiley, Hoboken",
journal = "Journal of the Science of Food and Agriculture",
title = "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study",
pages = "2505-2500",
number = "6",
volume = "102",
doi = "10.1002/jsfa.11591"
}

Bartolić, D., Mojović, M., Prokopijević, M., Đikanović, D., Kalauzi, A., Mutavdžić, D., Baosic, R.,& Radotić, K.. (2022). Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture
Wiley, Hoboken., 102(6), 2500-2505.
https://doi.org/10.1002/jsfa.11591

Bartolić D, Mojović M, Prokopijević M, Đikanović D, Kalauzi A, Mutavdžić D, Baosic R, Radotić K. Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study. in Journal of the Science of Food and Agriculture. 2022;102(6):2500-2505.
doi:10.1002/jsfa.11591 .

Bartolić, Dragana, Mojović, Miloš, Prokopijević, Miloš, Đikanović, Daniela, Kalauzi, Aleksandar, Mutavdžić, Dragosav, Baosic, Rada, Radotić, Ksenija, "Lignin and organic free radicals in maize (Zea mays L.) seeds in response to aflatoxin B-1 contamination: an optical and EPR spectroscopic study" in Journal of the Science of Food and Agriculture, 102, no. 6 (2022):2500-2505,
https://doi.org/10.1002/jsfa.11591 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1424
AB  - Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Spatial distribution of apoplastic antioxidative constituents in maize root
EP  - 828
IS  - 3
SP  - 818
VL  - 173
DO  - 10.1111/ppl.13476
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk",
year = "2021",
abstract = "Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Spatial distribution of apoplastic antioxidative constituents in maize root",
pages = "828-818",
number = "3",
volume = "173",
doi = "10.1111/ppl.13476"
}

Dragišić Maksimović, J., Mojović, M., Vučinić, Ž.,& Maksimović, V.. (2021). Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum
Wiley, Hoboken., 173(3), 818-828.
https://doi.org/10.1111/ppl.13476

Dragišić Maksimović J, Mojović M, Vučinić Ž, Maksimović V. Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum. 2021;173(3):818-828.
doi:10.1111/ppl.13476 .

Dragišić Maksimović, Jelena, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, "Spatial distribution of apoplastic antioxidative constituents in maize root" in Physiologia Plantarum, 173, no. 3 (2021):818-828,
https://doi.org/10.1111/ppl.13476 . .

TY  - JOUR
AU  - Zang, Lili
AU  - Morere-Le, Paven, Marie-Christine
AU  - Clochard, Thibault
AU  - Porcher, Alexis
AU  - Satour, Pascale
AU  - Mojović, Miloš
AU  - Vidović, Marija
AU  - Limami, Anis M.
AU  - Montrichard, Francoise
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1300
AB  - In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O-2(center dot-) -> H2O2 -> (OH)-O-center dot) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O-2(center dot-), H2O2 and (OH)-O-center dot in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O-2(center dot-) accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into (OH)-O-center dot. These changes impair the accumulation of H2O2 and then the accumulation of (OH)-O-center dot, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula
EP  - 373
SP  - 363
VL  - 146
DO  - 10.1016/j.plaphy.2019.11.006
ER  - 

@article{
author = "Zang, Lili and Morere-Le, Paven, Marie-Christine and Clochard, Thibault and Porcher, Alexis and Satour, Pascale and Mojović, Miloš and Vidović, Marija and Limami, Anis M. and Montrichard, Francoise",
year = "2020",
abstract = "In Medicago truncatula, nitrate, acting as a signal perceived by NITRATE TRANSPORTER1/PEPTIDE TRANSPORTER FAMILY 6.8 (MtNPF6.8), inhibits primary root growth through a reduction of root cell elongation. Since reactive oxygen species (ROS) produced and converted in root tip (O-2(center dot-) -> H2O2 -> (OH)-O-center dot) have been reported to control cell elongation, the impact of nitrate on the distribution of these ROS in the primary root of M. truncatula was analyzed. We found that nitrate reduced the content of O-2(center dot-), H2O2 and (OH)-O-center dot in the root tip of three wild type genotypes sensitive to nitrate (R108, DZA, A17), inhibition of root growth and O-2(center dot-) accumulation being highly correlated. Nitrate also modified the capacity of R108 root tip to produce or remove ROS. The ROS content decrease observed in R108 in response to nitrate is linked to changes in peroxidase activity (EC1.11.1.7) with an increase in peroxidative activity that scavenge H2O2 and a decrease in hydroxylic activity that converts H2O2 into (OH)-O-center dot. These changes impair the accumulation of H2O2 and then the accumulation of (OH)-O-center dot, the species responsible for cell wall loosening and cell elongation. Accordingly, nitrate inhibitory effect was abolished by externally added H2O2 or mimicked by KI, an H2O2 scavenger. In contrast, nitrate has no effect on ROS production or removal capacities in npf6.8-2, a knockdown line insensitive to nitrate, affected in the nitrate transporter MtNPF6.8 (in R108 background) by RNAi. Altogether, our data show that ROS are mediators acting downstream of MtNPF6.8 in the nitrate signaling pathway.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula",
pages = "373-363",
volume = "146",
doi = "10.1016/j.plaphy.2019.11.006"
}

Zang, L., Morere-Le, P. M., Clochard, T., Porcher, A., Satour, P., Mojović, M., Vidović, M., Limami, A. M.,& Montrichard, F.. (2020). Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 146, 363-373.
https://doi.org/10.1016/j.plaphy.2019.11.006

Zang L, Morere-Le PM, Clochard T, Porcher A, Satour P, Mojović M, Vidović M, Limami AM, Montrichard F. Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula. in Plant Physiology and Biochemistry. 2020;146:363-373.
doi:10.1016/j.plaphy.2019.11.006 .

Zang, Lili, Morere-Le, Paven, Marie-Christine, Clochard, Thibault, Porcher, Alexis, Satour, Pascale, Mojović, Miloš, Vidović, Marija, Limami, Anis M., Montrichard, Francoise, "Nitrate inhibits primary root growth by reducing accumulation of reactive oxygen species in the root tip in Medicago truncatula" in Plant Physiology and Biochemistry, 146 (2020):363-373,
https://doi.org/10.1016/j.plaphy.2019.11.006 . .

TY  - JOUR
AU  - Nakarada, Dura
AU  - Pejin, Boris
AU  - Tommonaro, Giuseppina
AU  - Mojović, Miloš
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1340
AB  - The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Liposome Research
T1  - Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol
EP  - 226
IS  - 3
SP  - 218
VL  - 30
DO  - 10.1080/08982104.2019.1625378
ER  - 

@article{
author = "Nakarada, Dura and Pejin, Boris and Tommonaro, Giuseppina and Mojović, Miloš",
year = "2020",
abstract = "The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge Dysidea avara. The antioxidant activity of water-insoluble avarol towards (OH)-O-center dot, O-2(center dot-) and NO center dot radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards (OH)-O-center dot, O-2(center dot-), NO center dot and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Liposome Research",
title = "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol",
pages = "226-218",
number = "3",
volume = "30",
doi = "10.1080/08982104.2019.1625378"
}

Nakarada, D., Pejin, B., Tommonaro, G.,& Mojović, M.. (2020). Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research
Taylor & Francis Ltd, Abingdon., 30(3), 218-226.
https://doi.org/10.1080/08982104.2019.1625378

Nakarada D, Pejin B, Tommonaro G, Mojović M. Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol. in Journal of Liposome Research. 2020;30(3):218-226.
doi:10.1080/08982104.2019.1625378 .

Nakarada, Dura, Pejin, Boris, Tommonaro, Giuseppina, Mojović, Miloš, "Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol" in Journal of Liposome Research, 30, no. 3 (2020):218-226,
https://doi.org/10.1080/08982104.2019.1625378 . .

TY  - JOUR
AU  - Nakarada, Dura
AU  - Pejin, Boris
AU  - Dimic, Dusan
AU  - Ivanovic-Sasic, Ana
AU  - Mojović, Zorica
AU  - Mojović, Miloš
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1206
AB  - The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV-Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV-Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety.
PB  - Springer, Dordrecht
T2  - Reaction Kinetics Mechanisms and Catalysis
T1  - Electrochemical and spectroscopic study of l-dopa interaction with avarol
EP  - 229
IS  - 1
SP  - 219
VL  - 127
DO  - 10.1007/s11144-019-01575-z
ER  - 

@article{
author = "Nakarada, Dura and Pejin, Boris and Dimic, Dusan and Ivanovic-Sasic, Ana and Mojović, Zorica and Mojović, Miloš",
year = "2019",
abstract = "The electrochemistry of catecholamine neurotransmitters and their precursor l-dopa has been widely studied due to their relevance as biologically important compounds. The detection of these compounds from aqueous solution is hindered by the coexistence of quinone or hydroquinone. However, it was suggested that quinones adsorbed on the electrode surface can enhance catechol detection. In order to estimate the degree of interaction between quinones and l-dopa, cyclic voltammetry and UV-Vis spectroscopic study was performed. A sesquiterpenoid hydroquinone, isolated from the marine sponge Dysidea avara (avarol), has been used in this study. The change of apparent heterogeneous rate constant with different avarol/l-dopa ratio indicated that charge transfer could be enhanced at some extent. In addition to this, the obtained results for avarol and hydroquinone (its structural element) were compared. UV-Vis spectroscopic analysis confirmed interaction between l-dopa and avarol or hydroquinone. Taken all together, the interaction of l-dopa was stronger with hydroquinone than with avarol, presumably reflecting the conformational restrains of avarol caused by its terpenoid moiety.",
publisher = "Springer, Dordrecht",
journal = "Reaction Kinetics Mechanisms and Catalysis",
title = "Electrochemical and spectroscopic study of l-dopa interaction with avarol",
pages = "229-219",
number = "1",
volume = "127",
doi = "10.1007/s11144-019-01575-z"
}

Nakarada, D., Pejin, B., Dimic, D., Ivanovic-Sasic, A., Mojović, Z.,& Mojović, M.. (2019). Electrochemical and spectroscopic study of l-dopa interaction with avarol. in Reaction Kinetics Mechanisms and Catalysis
Springer, Dordrecht., 127(1), 219-229.
https://doi.org/10.1007/s11144-019-01575-z

Nakarada D, Pejin B, Dimic D, Ivanovic-Sasic A, Mojović Z, Mojović M. Electrochemical and spectroscopic study of l-dopa interaction with avarol. in Reaction Kinetics Mechanisms and Catalysis. 2019;127(1):219-229.
doi:10.1007/s11144-019-01575-z .

Nakarada, Dura, Pejin, Boris, Dimic, Dusan, Ivanovic-Sasic, Ana, Mojović, Zorica, Mojović, Miloš, "Electrochemical and spectroscopic study of l-dopa interaction with avarol" in Reaction Kinetics Mechanisms and Catalysis, 127, no. 1 (2019):219-229,
https://doi.org/10.1007/s11144-019-01575-z . .

TY  - CONF
AU  - Bartolić, Dragana
AU  - Stanković, Mira
AU  - Mojović, Miloš
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2795
AB  - Cereal seeds are susceptible to infection by the opportunistic pathogen Aspergillus flavus that
consequently leads to the contamination with aflatoxins. In this work, EPR imaging was applied
for mapping redox state of the maize and wheat seeds contaminated with aflatoxin, through detection
and localization of paramagnetic spin probes in vivo. The sample recordings were made
in the L-frequency domain (1.1 GHz). The 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl
(3-Carbamoyl-PROXYL) spin probe was used for the estimation of changes in the redox state of the
aflatoxin-stressed seeds. The EPR-active probes were reduced by cellular reducing agents forming
EPR inactive species (hydroxylamines). The reduction rate, which among other factors depends
on the redox status of the cell, provides information about the redox environment in the region
of interest. A higher and more localized 3-Carbamoyl-PROXYL spin-active probe signal has been
found in uncontaminated seeds than in aflatoxin-stressed seeds. Our results imply that aflatoxin
contamination leads to the change in the seeds’ redox status which provides useful information
about its impact on seed metabolism.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology 22nd SPPS, Belgrade
T1  - Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging
EP  - 61
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2795
ER  - 

@conference{
author = "Bartolić, Dragana and Stanković, Mira and Mojović, Miloš and Maksimović, Vuk and Radotić, Ksenija",
year = "2018",
abstract = "Cereal seeds are susceptible to infection by the opportunistic pathogen Aspergillus flavus that
consequently leads to the contamination with aflatoxins. In this work, EPR imaging was applied
for mapping redox state of the maize and wheat seeds contaminated with aflatoxin, through detection
and localization of paramagnetic spin probes in vivo. The sample recordings were made
in the L-frequency domain (1.1 GHz). The 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl
(3-Carbamoyl-PROXYL) spin probe was used for the estimation of changes in the redox state of the
aflatoxin-stressed seeds. The EPR-active probes were reduced by cellular reducing agents forming
EPR inactive species (hydroxylamines). The reduction rate, which among other factors depends
on the redox status of the cell, provides information about the redox environment in the region
of interest. A higher and more localized 3-Carbamoyl-PROXYL spin-active probe signal has been
found in uncontaminated seeds than in aflatoxin-stressed seeds. Our results imply that aflatoxin
contamination leads to the change in the seeds’ redox status which provides useful information
about its impact on seed metabolism.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology 22nd SPPS, Belgrade",
title = "Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging",
pages = "61-61",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2795"
}

Bartolić, D., Stanković, M., Mojović, M., Maksimović, V.,& Radotić, K.. (2018). Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging. in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 61-61.
https://hdl.handle.net/21.15107/rcub_rimsi_2795

Bartolić D, Stanković M, Mojović M, Maksimović V, Radotić K. Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging. in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade. 2018;:61-61.
https://hdl.handle.net/21.15107/rcub_rimsi_2795 .

Bartolić, Dragana, Stanković, Mira, Mojović, Miloš, Maksimović, Vuk, Radotić, Ksenija, "Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging" in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade (2018):61-61,
https://hdl.handle.net/21.15107/rcub_rimsi_2795 .

TY  - JOUR
AU  - Glumac, Miodrag
AU  - Pejin, Boris
AU  - Karaman, Maja
AU  - Mojović, Miloš
AU  - Matavulj, Milan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1093
AB  - In vitro evaluation of total phenolic contents and antiradical activities of the lignicolous fungi Fomes fomentarius and Schizophyllum commune hydrodistilled extracts was the subject of this study. This preliminary screening included four free radical species evaluated by UV-vis (DPPH center dot, ABTS(center dot) and (NO)-N-center dot) and EPR (Asc(center dot)), respectively. According to the experimental data obtained, both F. fomentarius and S. commune hydrodistilled extracts may be considered as promising sources of phenolic natural products (157 and 138mg GAE/g d.e., respectively) and other bioactives showing good anti-DPPH (1.31g/mL) and anti-Asc (70.40%) radical activities, respectively, at in vitro conditions.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics
EP  - 107
IS  - 1
SP  - 104
VL  - 31
DO  - 10.1080/14786419.2016.1212036
ER  - 

@article{
author = "Glumac, Miodrag and Pejin, Boris and Karaman, Maja and Mojović, Miloš and Matavulj, Milan",
year = "2017",
abstract = "In vitro evaluation of total phenolic contents and antiradical activities of the lignicolous fungi Fomes fomentarius and Schizophyllum commune hydrodistilled extracts was the subject of this study. This preliminary screening included four free radical species evaluated by UV-vis (DPPH center dot, ABTS(center dot) and (NO)-N-center dot) and EPR (Asc(center dot)), respectively. According to the experimental data obtained, both F. fomentarius and S. commune hydrodistilled extracts may be considered as promising sources of phenolic natural products (157 and 138mg GAE/g d.e., respectively) and other bioactives showing good anti-DPPH (1.31g/mL) and anti-Asc (70.40%) radical activities, respectively, at in vitro conditions.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics",
pages = "107-104",
number = "1",
volume = "31",
doi = "10.1080/14786419.2016.1212036"
}

Glumac, M., Pejin, B., Karaman, M., Mojović, M.,& Matavulj, M.. (2017). Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 31(1), 104-107.
https://doi.org/10.1080/14786419.2016.1212036

Glumac M, Pejin B, Karaman M, Mojović M, Matavulj M. Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics. in Natural Product Research. 2017;31(1):104-107.
doi:10.1080/14786419.2016.1212036 .

Glumac, Miodrag, Pejin, Boris, Karaman, Maja, Mojović, Miloš, Matavulj, Milan, "Lignicolous fungi hydrodistilled extracts may represent a promising source of natural phenolics" in Natural Product Research, 31, no. 1 (2017):104-107,
https://doi.org/10.1080/14786419.2016.1212036 . .

TY  - JOUR
AU  - Dimitrić-Marković, Jasmina
AU  - Pejin, Boris
AU  - Milenković, Dejan
AU  - Amic, Dragan
AU  - Begović, Nebojša
AU  - Mojović, Miloš
AU  - Marković, Zoran S.
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1103
AB  - Naturally occurring flavonoids, delphinidin, pelargonidin and malvin, were investigated experimentally and theoretically for their ability to scavenge hydroxyl and nitric oxide radicals. Electron spin resonance (ESR) spectroscopy was used to determine antiradical activity of the selected compounds and M05-2X/6-311+G(d,p) level of theory for the calculation of reaction enthalpies related to three possible mechanisms of free radical scavenging activity, namely HAT, SET-PT and SPLET. The results obtained show that the molecules investigated reacted with hydroxyl radical via both HAT and SPLET in the solvents investigated. These results point to HAT as implausible for the reaction with nitric oxide radical in all the solvents investigated. SET-PT also proved to be thermodynamically unfavourable for all three molecules in the solvents considered.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms
EP  - 446
SP  - 440
VL  - 218
DO  - 10.1016/j.foodchem.2016.09.106
ER  - 

@article{
author = "Dimitrić-Marković, Jasmina and Pejin, Boris and Milenković, Dejan and Amic, Dragan and Begović, Nebojša and Mojović, Miloš and Marković, Zoran S.",
year = "2017",
abstract = "Naturally occurring flavonoids, delphinidin, pelargonidin and malvin, were investigated experimentally and theoretically for their ability to scavenge hydroxyl and nitric oxide radicals. Electron spin resonance (ESR) spectroscopy was used to determine antiradical activity of the selected compounds and M05-2X/6-311+G(d,p) level of theory for the calculation of reaction enthalpies related to three possible mechanisms of free radical scavenging activity, namely HAT, SET-PT and SPLET. The results obtained show that the molecules investigated reacted with hydroxyl radical via both HAT and SPLET in the solvents investigated. These results point to HAT as implausible for the reaction with nitric oxide radical in all the solvents investigated. SET-PT also proved to be thermodynamically unfavourable for all three molecules in the solvents considered.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms",
pages = "446-440",
volume = "218",
doi = "10.1016/j.foodchem.2016.09.106"
}

Dimitrić-Marković, J., Pejin, B., Milenković, D., Amic, D., Begović, N., Mojović, M.,& Marković, Z. S.. (2017). Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms. in Food Chemistry
Elsevier Sci Ltd, Oxford., 218, 440-446.
https://doi.org/10.1016/j.foodchem.2016.09.106

Dimitrić-Marković J, Pejin B, Milenković D, Amic D, Begović N, Mojović M, Marković ZS. Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms. in Food Chemistry. 2017;218:440-446.
doi:10.1016/j.foodchem.2016.09.106 .

Dimitrić-Marković, Jasmina, Pejin, Boris, Milenković, Dejan, Amic, Dragan, Begović, Nebojša, Mojović, Miloš, Marković, Zoran S., "Antiradical activity of delphinidin, pelargonidin and malvin towards hydroxyl and nitric oxide radicals: The energy requirements calculations as a prediction of the possible antiradical mechanisms" in Food Chemistry, 218 (2017):440-446,
https://doi.org/10.1016/j.foodchem.2016.09.106 . .

TY  - JOUR
AU  - Morina, Filis
AU  - Takahama, Umeo
AU  - Mojović, Miloš
AU  - Popovic-Bijelic, Ana
AU  - Veljović-Jovanović, Sonja
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/939
AB  - Catechins are transformed into dinitrosocatechins (diNOcats) and then oxidized to the quinones by salivary nitrite under conditions simulating the stomach. This manuscript deals with formation of stable radicals in the NO group of diNOcat during nitrite-induced oxidation of (+)-catechin and diNOcat at pH 2. We postulated two mechanisms for the stable radical formation; one is nitrous acid-induced oxidation of diNOcat in the A-ring, and the other intermolecular charge transfer from the A-ring of diNOcat and/or diNOcat quinone to the quinone moiety of the B-ring of diNOcat quinone. In addition, an unstable phenoxyl radical, which might be transformed into quinone, was also produced, accompanying the formation of the stable radical on the NO group. Taking the above results into account, we mainly focus on the adverse effects of the radicals and quinone, which may be produced from (+)-catechin in the stomach under the conditions of high salivary nitrite concentrations.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Formation of stable radicals in catechin/nitrous acid systems: Participation of dinitrosocatechin
EP  - 1122
SP  - 1116
VL  - 194
DO  - 10.1016/j.foodchem.2015.08.081
ER  - 

@article{
author = "Morina, Filis and Takahama, Umeo and Mojović, Miloš and Popovic-Bijelic, Ana and Veljović-Jovanović, Sonja",
year = "2016",
abstract = "Catechins are transformed into dinitrosocatechins (diNOcats) and then oxidized to the quinones by salivary nitrite under conditions simulating the stomach. This manuscript deals with formation of stable radicals in the NO group of diNOcat during nitrite-induced oxidation of (+)-catechin and diNOcat at pH 2. We postulated two mechanisms for the stable radical formation; one is nitrous acid-induced oxidation of diNOcat in the A-ring, and the other intermolecular charge transfer from the A-ring of diNOcat and/or diNOcat quinone to the quinone moiety of the B-ring of diNOcat quinone. In addition, an unstable phenoxyl radical, which might be transformed into quinone, was also produced, accompanying the formation of the stable radical on the NO group. Taking the above results into account, we mainly focus on the adverse effects of the radicals and quinone, which may be produced from (+)-catechin in the stomach under the conditions of high salivary nitrite concentrations.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Formation of stable radicals in catechin/nitrous acid systems: Participation of dinitrosocatechin",
pages = "1122-1116",
volume = "194",
doi = "10.1016/j.foodchem.2015.08.081"
}

Morina, F., Takahama, U., Mojović, M., Popovic-Bijelic, A.,& Veljović-Jovanović, S.. (2016). Formation of stable radicals in catechin/nitrous acid systems: Participation of dinitrosocatechin. in Food Chemistry
Elsevier Sci Ltd, Oxford., 194, 1116-1122.
https://doi.org/10.1016/j.foodchem.2015.08.081

Morina F, Takahama U, Mojović M, Popovic-Bijelic A, Veljović-Jovanović S. Formation of stable radicals in catechin/nitrous acid systems: Participation of dinitrosocatechin. in Food Chemistry. 2016;194:1116-1122.
doi:10.1016/j.foodchem.2015.08.081 .

Morina, Filis, Takahama, Umeo, Mojović, Miloš, Popovic-Bijelic, Ana, Veljović-Jovanović, Sonja, "Formation of stable radicals in catechin/nitrous acid systems: Participation of dinitrosocatechin" in Food Chemistry, 194 (2016):1116-1122,
https://doi.org/10.1016/j.foodchem.2015.08.081 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
AU  - Maksimović, Vuk
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1804
AB  - The effect of excess nutrient levels of manganese (Mn, 50 and 100 M) on the growth inhibition 
and the appearance of Mn-toxicity symptoms in the leaves was studied in cucumber plants 
(Cucumis sativus L. cv. Chinese long). Silicon (Si), when supplied as 1.5 mM silicic acid, clearly 
decreased symptoms of Mn-toxicity despite approximately the same total Mn content in the 
leaves. In treated plants, Si improves growth and biomass production compared with that of non Si treated plants. Inert deposition of Si in the leaf cell walls of cucumber (a Si-accumulating 
species) enhanced cell wall stability. The mechanism of Si protection is proposed to act by Si induced compartmentation of Mn hence increasing Mn2+
-binding sites in the cell wall (e.g. Mn silicate polymers) finally resulting in decreased toxic free Mn within the plant tissue rather than 
decrease of Mn uptake. These results suggest that Si nutrition can improve the phytoextraction 
potential of plants due to enhanced metal tolerance in leaf tissues
PB  - Inženjersko društvo za koroziju, Beograd
T2  - Zaštita materijala
T1  - Silicon facilitates manganese phytoextraction by cucumber (Cucumis sativus L.)
EP  - 429
IS  - 3
SP  - 424
VL  - 57
DO  - 10.5937/ZasMat1603424D
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Maksimović, Vuk",
year = "2016",
abstract = "The effect of excess nutrient levels of manganese (Mn, 50 and 100 M) on the growth inhibition 
and the appearance of Mn-toxicity symptoms in the leaves was studied in cucumber plants 
(Cucumis sativus L. cv. Chinese long). Silicon (Si), when supplied as 1.5 mM silicic acid, clearly 
decreased symptoms of Mn-toxicity despite approximately the same total Mn content in the 
leaves. In treated plants, Si improves growth and biomass production compared with that of non Si treated plants. Inert deposition of Si in the leaf cell walls of cucumber (a Si-accumulating 
species) enhanced cell wall stability. The mechanism of Si protection is proposed to act by Si induced compartmentation of Mn hence increasing Mn2+
-binding sites in the cell wall (e.g. Mn silicate polymers) finally resulting in decreased toxic free Mn within the plant tissue rather than 
decrease of Mn uptake. These results suggest that Si nutrition can improve the phytoextraction 
potential of plants due to enhanced metal tolerance in leaf tissues",
publisher = "Inženjersko društvo za koroziju, Beograd",
journal = "Zaštita materijala",
title = "Silicon facilitates manganese phytoextraction by cucumber (Cucumis sativus L.)",
pages = "429-424",
number = "3",
volume = "57",
doi = "10.5937/ZasMat1603424D"
}

Dragišić Maksimović, J., Mojović, M.,& Maksimović, V.. (2016). Silicon facilitates manganese phytoextraction by cucumber (Cucumis sativus L.). in Zaštita materijala
Inženjersko društvo za koroziju, Beograd., 57(3), 424-429.
https://doi.org/10.5937/ZasMat1603424D

Dragišić Maksimović J, Mojović M, Maksimović V. Silicon facilitates manganese phytoextraction by cucumber (Cucumis sativus L.). in Zaštita materijala. 2016;57(3):424-429.
doi:10.5937/ZasMat1603424D .

Dragišić Maksimović, Jelena, Mojović, Miloš, Maksimović, Vuk, "Silicon facilitates manganese phytoextraction by cucumber (Cucumis sativus L.)" in Zaštita materijala, 57, no. 3 (2016):424-429,
https://doi.org/10.5937/ZasMat1603424D . .

TY  - CONF
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
AU  - Pešić, Milica
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1837
AB  - Radiotherapy is commonly used for the treatment of colon cancer. Additionally, various antioxidant agents, such as nanoparticles of cerium oxide (nanoceria, nanoCeO2), are being investigated as the adjunctive therapy. CeO2 shows the activity of superoxide dismutase (SOD) and catalase mimetic activity, which are responsible for its antioxidant behavior. However, prolonged exposure to higher concentrations of nanoceria cells leads to the dysfunction of the antioxidant system, induction of intracellular oxidative stress and subsequent cytotoxicity in cells. The aim of this study was to investigate the cytotoxic effect of nanoparticles of CeO2 in colon cancer cells (HT-29) and the development of appropriate methods to monitor this effect. MTT test showed a lower cytotoxic activity than the standard cisplatin (concentrations used between 0-100 µM). EPR detection of total radicals using a spin probe TEMPONE and detection of OH radicals by fluorescence spectroscopy have shown that cellular mechanisms for the removal of free radicals are less efficient in cells treated with nanoceria, which explains the cytotoxicity of these nanoparticles on colon cancer cells.
PB  - Serbian Chemical Society
C3  - Third Conference of Young Chemists of Serbia 3KMHS-3CYCS
T1  - Cytotoxic effect of nanoceria on colon cancer cells (HT-29)
EP  - 66
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1837
ER  - 

@conference{
author = "Milenković, Ivana and Radotić, Ksenija and Mojović, Miloš and Pešić, Milica",
year = "2015",
abstract = "Radiotherapy is commonly used for the treatment of colon cancer. Additionally, various antioxidant agents, such as nanoparticles of cerium oxide (nanoceria, nanoCeO2), are being investigated as the adjunctive therapy. CeO2 shows the activity of superoxide dismutase (SOD) and catalase mimetic activity, which are responsible for its antioxidant behavior. However, prolonged exposure to higher concentrations of nanoceria cells leads to the dysfunction of the antioxidant system, induction of intracellular oxidative stress and subsequent cytotoxicity in cells. The aim of this study was to investigate the cytotoxic effect of nanoparticles of CeO2 in colon cancer cells (HT-29) and the development of appropriate methods to monitor this effect. MTT test showed a lower cytotoxic activity than the standard cisplatin (concentrations used between 0-100 µM). EPR detection of total radicals using a spin probe TEMPONE and detection of OH radicals by fluorescence spectroscopy have shown that cellular mechanisms for the removal of free radicals are less efficient in cells treated with nanoceria, which explains the cytotoxicity of these nanoparticles on colon cancer cells.",
publisher = "Serbian Chemical Society",
journal = "Third Conference of Young Chemists of Serbia 3KMHS-3CYCS",
title = "Cytotoxic effect of nanoceria on colon cancer cells (HT-29)",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1837"
}

Milenković, I., Radotić, K., Mojović, M.,& Pešić, M.. (2015). Cytotoxic effect of nanoceria on colon cancer cells (HT-29). in Third Conference of Young Chemists of Serbia 3KMHS-3CYCS
Serbian Chemical Society., 66-66.
https://hdl.handle.net/21.15107/rcub_rimsi_1837

Milenković I, Radotić K, Mojović M, Pešić M. Cytotoxic effect of nanoceria on colon cancer cells (HT-29). in Third Conference of Young Chemists of Serbia 3KMHS-3CYCS. 2015;:66-66.
https://hdl.handle.net/21.15107/rcub_rimsi_1837 .

Milenković, Ivana, Radotić, Ksenija, Mojović, Miloš, Pešić, Milica, "Cytotoxic effect of nanoceria on colon cancer cells (HT-29)" in Third Conference of Young Chemists of Serbia 3KMHS-3CYCS (2015):66-66,
https://hdl.handle.net/21.15107/rcub_rimsi_1837 .

TY  - JOUR
AU  - Pesic, Milica
AU  - Podolski-Renic, Ana
AU  - Stojković, Sonja
AU  - Matović, Branko
AU  - Zmejkoski, Danica
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Pavicević, Aleksandra
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
AU  - Milenković, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/931
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity
EP  - 93
SP  - 85
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
ER  - 

@article{
author = "Pesic, Milica and Podolski-Renic, Ana and Stojković, Sonja and Matović, Branko and Zmejkoski, Danica and Kojić, Vesna and Bogdanović, Gordana and Pavicević, Aleksandra and Mojović, Miloš and Savić, Aleksandar G and Milenković, Ivana and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity",
pages = "93-85",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013"
}

Pesic, M., Podolski-Renic, A., Stojković, S., Matović, B., Zmejkoski, D., Kojić, V., Bogdanović, G., Pavicević, A., Mojović, M., Savić, A. G., Milenković, I., Kalauzi, A.,& Radotić, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pesic M, Podolski-Renic A, Stojković S, Matović B, Zmejkoski D, Kojić V, Bogdanović G, Pavicević A, Mojović M, Savić AG, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pesic, Milica, Podolski-Renic, Ana, Stojković, Sonja, Matović, Branko, Zmejkoski, Danica, Kojić, Vesna, Bogdanović, Gordana, Pavicević, Aleksandra, Mojović, Miloš, Savić, Aleksandar G, Milenković, Ivana, Kalauzi, Aleksandar, Radotić, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Poledica, Milena M
AU  - Mutavdžić, Dragosav
AU  - Mojović, Miloš
AU  - Radivojević, Dragan
AU  - Milivojević, Jasminka M
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/829
AB  - Bioclimatic air ionisation system (BI) works by neutralising air pollutants and microorganisms by means of oxidation with "activated oxygen". We investigated the effects of storage on changes in weight loss, chemical and sensory fruit properties in eight cultivars of strawberries (Fragaria x ananassa Duch.). All cultivars were evaluated for their standard parameters of quality (soluble solids content, total acidity, vitamin C content, total antioxidant activity - TAC, total phenolic and anthocyanins content) at different store conditions: fresh fruits-control, cold stored (at 4 A degrees C) fruits without controlled atmospheres and cold stored (at 4 A degrees C) fruits in BI. The present study outlines that anthocyanins of the strawberries stored in BI were subjected to significant degradation. These strawberries have prolonged shelf-life accompanied by weight loss reduction, TAC increment, and sensory properties improvement in tested cultivars, retaining other nutritional fruit qualities.
PB  - Springer, Dordrecht
T2  - Plant Foods for Human Nutrition
T1  - Variation in Nutritional Quality and Chemical Composition of Fresh Strawberry Fruit: Combined Effect of Cultivar and Storage
EP  - 84
IS  - 1
SP  - 77
VL  - 70
DO  - 10.1007/s11130-014-0464-3
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Poledica, Milena M and Mutavdžić, Dragosav and Mojović, Miloš and Radivojević, Dragan and Milivojević, Jasminka M",
year = "2015",
abstract = "Bioclimatic air ionisation system (BI) works by neutralising air pollutants and microorganisms by means of oxidation with "activated oxygen". We investigated the effects of storage on changes in weight loss, chemical and sensory fruit properties in eight cultivars of strawberries (Fragaria x ananassa Duch.). All cultivars were evaluated for their standard parameters of quality (soluble solids content, total acidity, vitamin C content, total antioxidant activity - TAC, total phenolic and anthocyanins content) at different store conditions: fresh fruits-control, cold stored (at 4 A degrees C) fruits without controlled atmospheres and cold stored (at 4 A degrees C) fruits in BI. The present study outlines that anthocyanins of the strawberries stored in BI were subjected to significant degradation. These strawberries have prolonged shelf-life accompanied by weight loss reduction, TAC increment, and sensory properties improvement in tested cultivars, retaining other nutritional fruit qualities.",
publisher = "Springer, Dordrecht",
journal = "Plant Foods for Human Nutrition",
title = "Variation in Nutritional Quality and Chemical Composition of Fresh Strawberry Fruit: Combined Effect of Cultivar and Storage",
pages = "84-77",
number = "1",
volume = "70",
doi = "10.1007/s11130-014-0464-3"
}

Dragišić Maksimović, J., Poledica, M. M., Mutavdžić, D., Mojović, M., Radivojević, D.,& Milivojević, J. M.. (2015). Variation in Nutritional Quality and Chemical Composition of Fresh Strawberry Fruit: Combined Effect of Cultivar and Storage. in Plant Foods for Human Nutrition
Springer, Dordrecht., 70(1), 77-84.
https://doi.org/10.1007/s11130-014-0464-3

Dragišić Maksimović J, Poledica MM, Mutavdžić D, Mojović M, Radivojević D, Milivojević JM. Variation in Nutritional Quality and Chemical Composition of Fresh Strawberry Fruit: Combined Effect of Cultivar and Storage. in Plant Foods for Human Nutrition. 2015;70(1):77-84.
doi:10.1007/s11130-014-0464-3 .

Dragišić Maksimović, Jelena, Poledica, Milena M, Mutavdžić, Dragosav, Mojović, Miloš, Radivojević, Dragan, Milivojević, Jasminka M, "Variation in Nutritional Quality and Chemical Composition of Fresh Strawberry Fruit: Combined Effect of Cultivar and Storage" in Plant Foods for Human Nutrition, 70, no. 1 (2015):77-84,
https://doi.org/10.1007/s11130-014-0464-3 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Nakarada, Dura
AU  - Novaković, Miroslav
AU  - Tešević, Vele
AU  - Savić, Aleksandar G
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/773
AB  - Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata
EP  - 1475
IS  - 18
SP  - 1471
VL  - 28
DO  - 10.1080/14786419.2014.905565
ER  - 

@article{
author = "Pejin, Boris and Nakarada, Dura and Novaković, Miroslav and Tešević, Vele and Savić, Aleksandar G and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
abstract = "Two volatile samples of the bryozoan Hyalinella punctata (seasonally collected from the same locality) were isolated (hydrodistillation, Clevenger apparatus), identified (GC and GC-MS) and screened in vitro (EPR) for anti-hydroxyl radical activity. The main components of sample 1 (2-ethyl-1-hexanol 37.00%, dodecanol 21.40% and hexanal 8.40%) and sample 2 (2-ethyl-1-hexanol 30.50%, 7-tridecanol 24.60% and 1-hexadecanol 11.80%) were relatively similar. However, more components were present in the sample 2 (17 and 25, respectively). EPR measurements indicated significant anti-hydroxyl radical activity of the both samples (75.00 +/- 6.00% and 87.00 +/- 8.00%, respectively) whereas the generation of other types of free radicals in reaction with hydroxyl radicals was not observed. According to the best of our knowledge, this is the first report of 31 organic compounds from the phylum Bryozoa. Alcohols, aldehydes, ketones, esters and ethers of lower molecular mass appear to be characteristic for the volatiles of these organisms commonly known as moss animals.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata",
pages = "1475-1471",
number = "18",
volume = "28",
doi = "10.1080/14786419.2014.905565"
}

Pejin, B., Nakarada, D., Novaković, M., Tešević, V., Savić, A. G., Radotić, K.,& Mojović, M.. (2014). Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(18), 1471-1475.
https://doi.org/10.1080/14786419.2014.905565

Pejin B, Nakarada D, Novaković M, Tešević V, Savić AG, Radotić K, Mojović M. Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata. in Natural Product Research. 2014;28(18):1471-1475.
doi:10.1080/14786419.2014.905565 .

Pejin, Boris, Nakarada, Dura, Novaković, Miroslav, Tešević, Vele, Savić, Aleksandar G, Radotić, Ksenija, Mojović, Miloš, "Antioxidant volatiles of the freshwater bryozoan Hyalinella punctata" in Natural Product Research, 28, no. 18 (2014):1471-1475,
https://doi.org/10.1080/14786419.2014.905565 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ciric, Ana D
AU  - Nikolic, Milos MN
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/759
AB  - The antiradical activity of phytol was evaluated by electron paramagnetic resonance towards hydroxyl radical (root OH), superoxide anion radical (), methoxy radical (root CH2OH), carbon-dioxide anion radical (), as well as towards nitric-oxide radical (root NO) and 2,2-diphenyl-1-picrylhydrazyl (root DPPH) radical. It reduced the production of all tested radicals showing more promising activity against , root CH2OH and root DPPH radicals (56%, 50% and 48%, respectively) in comparison with root NO, and root OH radicals (38%, 23% and 15%, respectively). The antimicrobial activity of phytol was evaluated by the microdilution method against eight bacterial and eight fungal strains. To varying degrees, it was proven to be active against all tested bacteria and fungi (MIC 0.003-0.038mg/mL and MBC 0.013-0.052mg/mL, MIC 0.008-0.016mg/mL and MFC 0.090-0.520mg/mL, respectively). According to the obtained results, medical foods containing phytol may support development of new therapies for heart disease.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Further in vitro evaluation of antiradical and antimicrobial activities of phytol
EP  - 376
IS  - 6
SP  - 372
VL  - 28
DO  - 10.1080/14786419.2013.869692
ER  - 

@article{
author = "Pejin, Boris and Savić, Aleksandar G and Soković, Marina and Glamočlija, Jasmina and Ciric, Ana D and Nikolic, Milos MN and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
abstract = "The antiradical activity of phytol was evaluated by electron paramagnetic resonance towards hydroxyl radical (root OH), superoxide anion radical (), methoxy radical (root CH2OH), carbon-dioxide anion radical (), as well as towards nitric-oxide radical (root NO) and 2,2-diphenyl-1-picrylhydrazyl (root DPPH) radical. It reduced the production of all tested radicals showing more promising activity against , root CH2OH and root DPPH radicals (56%, 50% and 48%, respectively) in comparison with root NO, and root OH radicals (38%, 23% and 15%, respectively). The antimicrobial activity of phytol was evaluated by the microdilution method against eight bacterial and eight fungal strains. To varying degrees, it was proven to be active against all tested bacteria and fungi (MIC 0.003-0.038mg/mL and MBC 0.013-0.052mg/mL, MIC 0.008-0.016mg/mL and MFC 0.090-0.520mg/mL, respectively). According to the obtained results, medical foods containing phytol may support development of new therapies for heart disease.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Further in vitro evaluation of antiradical and antimicrobial activities of phytol",
pages = "376-372",
number = "6",
volume = "28",
doi = "10.1080/14786419.2013.869692"
}

Pejin, B., Savić, A. G., Soković, M., Glamočlija, J., Ciric, A. D., Nikolic, M. M., Radotić, K.,& Mojović, M.. (2014). Further in vitro evaluation of antiradical and antimicrobial activities of phytol. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(6), 372-376.
https://doi.org/10.1080/14786419.2013.869692

Pejin B, Savić AG, Soković M, Glamočlija J, Ciric AD, Nikolic MM, Radotić K, Mojović M. Further in vitro evaluation of antiradical and antimicrobial activities of phytol. in Natural Product Research. 2014;28(6):372-376.
doi:10.1080/14786419.2013.869692 .

Pejin, Boris, Savić, Aleksandar G, Soković, Marina, Glamočlija, Jasmina, Ciric, Ana D, Nikolic, Milos MN, Radotić, Ksenija, Mojović, Miloš, "Further in vitro evaluation of antiradical and antimicrobial activities of phytol" in Natural Product Research, 28, no. 6 (2014):372-376,
https://doi.org/10.1080/14786419.2013.869692 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Petković, Milena M
AU  - Radotić, Ksenija
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/752
AB  - Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti-hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti-hydroxyl radical activity of the FOS 1-kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations. A significant anti-hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease.
PB  - Wiley, Hoboken
T2  - International Journal of Food Science and Technology
T1  - In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches
EP  - 1505
IS  - 6
SP  - 1500
VL  - 49
DO  - 10.1111/ijfs.12445
ER  - 

@article{
author = "Pejin, Boris and Savić, Aleksandar G and Petković, Milena M and Radotić, Ksenija and Mojović, Miloš",
year = "2014",
abstract = "Fructooligosaccharides (FOS) are bioactive oligosaccharide fructans with beneficial health effects. Anti-hydroxyl radical activity is an important factor in the description of antioxidant capacity of any natural product. The aim of this study was to estimate in vitro anti-hydroxyl radical activity of the FOS 1-kestose and nystose by electron paramagnetic resonance spectroscopy (EPR) and fluorescence spectroscopy (FS) followed by a theoretical approach based on quantum chemistry calculations. A significant anti-hydroxyl radical potential of both compounds was observed (72% and 78% by EPR and 69% and 74% by FS, respectively), indicating the nystose to be a more active natural product. In addition, the computational results have confirmed that nystose follows the same pattern previously shown for 1-kestose, that is, that carbohydrates can react with hydroxyl radical. It is well known that FOS belong to cardioprotective nutraceuticals, so the study may be of some interest to research in heart disease.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Food Science and Technology",
title = "In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches",
pages = "1505-1500",
number = "6",
volume = "49",
doi = "10.1111/ijfs.12445"
}

Pejin, B., Savić, A. G., Petković, M. M., Radotić, K.,& Mojović, M.. (2014). In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches. in International Journal of Food Science and Technology
Wiley, Hoboken., 49(6), 1500-1505.
https://doi.org/10.1111/ijfs.12445

Pejin B, Savić AG, Petković MM, Radotić K, Mojović M. In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches. in International Journal of Food Science and Technology. 2014;49(6):1500-1505.
doi:10.1111/ijfs.12445 .

Pejin, Boris, Savić, Aleksandar G, Petković, Milena M, Radotić, Ksenija, Mojović, Miloš, "In vitro anti-hydroxyl radical activity of the fructooligosaccharides 1-kestose and nystose using spectroscopic and computational approaches" in International Journal of Food Science and Technology, 49, no. 6 (2014):1500-1505,
https://doi.org/10.1111/ijfs.12445 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Živanović, Branka  D.
AU  - Maksimović, Vuk
AU  - Mojović, Miloš
AU  - Nikolic, Miroslav
AU  - Vučinić, Željko
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/751
AB  - Apoplastic fluid was extracted from maize (Zea mays L) roots using two procedures: collection from the surface of intact plant roots by filter paper strips (AF) or vacuum infiltration and/or centrifugation from excised root segments (AWF). The content of cytoplasmic marker (glucose-6-phosphate, G-6-P) and antioxidative components (enzymes, organic acids, phenolics, sugars, ROS) were compared in the extracts. The results obtained demonstrate that AF was completely free of G-6-P, as opposed to AWF where the cytoplasmic constituent was detected even at mildest centrifugation (200 x g). Isoelectric focusing of POD and SOD shows the presence of cytoplasmic isoforms in AWF, and HPLC of sugars and phenolics a much more complex composition of AWF, due to cytoplasmic contamination. Organic acid composition differed in the two extracts, much higher concentrations of malic acid being registered in AF, while oxalic acid due to intracellular contamination being present only in AWF. EPR spectroscopy of DEPMPO spin trap in the extracts showed persistent generation of hydroxyl radical adduct in AF. The results obtained argue in favor of the filter strip method for the root apoplastic fluid extraction, avoiding the problems of cytoplasmic contamination and dilution and enabling concentration measurements in minute regions of the root.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Filter strip as a method of choice for apoplastic fluid extraction from maize roots
EP  - 58
SP  - 49
VL  - 223
DO  - 10.1016/j.plantsci.2014.03.009
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Živanović, Branka  D. and Maksimović, Vuk and Mojović, Miloš and Nikolic, Miroslav and Vučinić, Željko",
year = "2014",
abstract = "Apoplastic fluid was extracted from maize (Zea mays L) roots using two procedures: collection from the surface of intact plant roots by filter paper strips (AF) or vacuum infiltration and/or centrifugation from excised root segments (AWF). The content of cytoplasmic marker (glucose-6-phosphate, G-6-P) and antioxidative components (enzymes, organic acids, phenolics, sugars, ROS) were compared in the extracts. The results obtained demonstrate that AF was completely free of G-6-P, as opposed to AWF where the cytoplasmic constituent was detected even at mildest centrifugation (200 x g). Isoelectric focusing of POD and SOD shows the presence of cytoplasmic isoforms in AWF, and HPLC of sugars and phenolics a much more complex composition of AWF, due to cytoplasmic contamination. Organic acid composition differed in the two extracts, much higher concentrations of malic acid being registered in AF, while oxalic acid due to intracellular contamination being present only in AWF. EPR spectroscopy of DEPMPO spin trap in the extracts showed persistent generation of hydroxyl radical adduct in AF. The results obtained argue in favor of the filter strip method for the root apoplastic fluid extraction, avoiding the problems of cytoplasmic contamination and dilution and enabling concentration measurements in minute regions of the root.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Filter strip as a method of choice for apoplastic fluid extraction from maize roots",
pages = "58-49",
volume = "223",
doi = "10.1016/j.plantsci.2014.03.009"
}

Dragišić Maksimović, J., Živanović, Branka  D., Maksimović, V., Mojović, M., Nikolic, M.,& Vučinić, Ž.. (2014). Filter strip as a method of choice for apoplastic fluid extraction from maize roots. in Plant Science
Elsevier Ireland Ltd, Clare., 223, 49-58.
https://doi.org/10.1016/j.plantsci.2014.03.009

Dragišić Maksimović J, Živanović, Branka  D., Maksimović V, Mojović M, Nikolic M, Vučinić Ž. Filter strip as a method of choice for apoplastic fluid extraction from maize roots. in Plant Science. 2014;223:49-58.
doi:10.1016/j.plantsci.2014.03.009 .

Dragišić Maksimović, Jelena, Živanović, Branka  D., Maksimović, Vuk, Mojović, Miloš, Nikolic, Miroslav, Vučinić, Željko, "Filter strip as a method of choice for apoplastic fluid extraction from maize roots" in Plant Science, 223 (2014):49-58,
https://doi.org/10.1016/j.plantsci.2014.03.009 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Hegediš, Aleksandar
AU  - Karaman, Ivo
AU  - Horvatović, Mladen
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/770
AB  - The antiradical activity of the freshwater bryozoan Hyalinella punctata water extracts (two samples, seasonal collection) was evaluated by using electron paramagnetic resonance spectroscopy against hydroxyl (zOH), superoxide anion (center dot O-2(-)), methoxy (center dot CH2OH), carbon-dioxide anion (center dot CO2-), nitric-oxide (center dot NO) and 2,2-diphenyl-1-picrylhydrazyl (center dot DPPH) radicals. The extracts reduced the production of all tested radicals but to a varying degree. The better activity was observed against center dot CO2- and center dot CH2OH radicals (54 +/- 5% and 44 +/- 4%, and 58 +/- 6% and 22 +/- 2%, respectively) than towards center dot DPPH, center dot NO, center dot OH and center dot O-2(-) radicals (59 +/- 6% and 1.0 +/- 0.1%, 46 +/- 5% and 14 +/- 1%, 7.0 +/- 0.5% and 34 +/- 3%, and 33 +/- 3% and 0%, respectively). FTIR spectra of the both extracts indicate the presence of cyclic peptides and polypeptides which might be responsible for the observed activity. According to the experimental data obtained, H. punctata water extract may be considered as a novel promising resource of natural products with anti center dot CO2- radical activity.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity
EP  - 2060
IS  - 22
SP  - 2057
VL  - 28
DO  - 10.1080/14786419.2014.921788
ER  - 

@article{
author = "Pejin, Boris and Savić, Aleksandar G and Hegediš, Aleksandar and Karaman, Ivo and Horvatović, Mladen and Mojović, Miloš",
year = "2014",
abstract = "The antiradical activity of the freshwater bryozoan Hyalinella punctata water extracts (two samples, seasonal collection) was evaluated by using electron paramagnetic resonance spectroscopy against hydroxyl (zOH), superoxide anion (center dot O-2(-)), methoxy (center dot CH2OH), carbon-dioxide anion (center dot CO2-), nitric-oxide (center dot NO) and 2,2-diphenyl-1-picrylhydrazyl (center dot DPPH) radicals. The extracts reduced the production of all tested radicals but to a varying degree. The better activity was observed against center dot CO2- and center dot CH2OH radicals (54 +/- 5% and 44 +/- 4%, and 58 +/- 6% and 22 +/- 2%, respectively) than towards center dot DPPH, center dot NO, center dot OH and center dot O-2(-) radicals (59 +/- 6% and 1.0 +/- 0.1%, 46 +/- 5% and 14 +/- 1%, 7.0 +/- 0.5% and 34 +/- 3%, and 33 +/- 3% and 0%, respectively). FTIR spectra of the both extracts indicate the presence of cyclic peptides and polypeptides which might be responsible for the observed activity. According to the experimental data obtained, H. punctata water extract may be considered as a novel promising resource of natural products with anti center dot CO2- radical activity.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity",
pages = "2060-2057",
number = "22",
volume = "28",
doi = "10.1080/14786419.2014.921788"
}

Pejin, B., Savić, A. G., Hegediš, A., Karaman, I., Horvatović, M.,& Mojović, M.. (2014). A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(22), 2057-2060.
https://doi.org/10.1080/14786419.2014.921788

Pejin B, Savić AG, Hegediš A, Karaman I, Horvatović M, Mojović M. A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity. in Natural Product Research. 2014;28(22):2057-2060.
doi:10.1080/14786419.2014.921788 .

Pejin, Boris, Savić, Aleksandar G, Hegediš, Aleksandar, Karaman, Ivo, Horvatović, Mladen, Mojović, Miloš, "A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity" in Natural Product Research, 28, no. 22 (2014):2057-2060,
https://doi.org/10.1080/14786419.2014.921788 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/805
AB  - This article covers the 2003-2012 literature published for marine natural products from the phylum Cnidaria. The focus is on new and highly potent antitumour substances, together with details related to the organism sourced. It describes 12 promising bioactives isolated from 7 species.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - Novel and highly potent antitumour natural products from cnidarians of marine origin
EP  - 2244
IS  - 24
SP  - 2237
VL  - 28
DO  - 10.1080/14786419.2014.934241
ER  - 

@article{
author = "Pejin, Boris and Mojović, Miloš and Savić, Aleksandar G",
year = "2014",
abstract = "This article covers the 2003-2012 literature published for marine natural products from the phylum Cnidaria. The focus is on new and highly potent antitumour substances, together with details related to the organism sourced. It describes 12 promising bioactives isolated from 7 species.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "Novel and highly potent antitumour natural products from cnidarians of marine origin",
pages = "2244-2237",
number = "24",
volume = "28",
doi = "10.1080/14786419.2014.934241"
}

Pejin, B., Mojović, M.,& Savić, A. G.. (2014). Novel and highly potent antitumour natural products from cnidarians of marine origin. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 28(24), 2237-2244.
https://doi.org/10.1080/14786419.2014.934241

Pejin B, Mojović M, Savić AG. Novel and highly potent antitumour natural products from cnidarians of marine origin. in Natural Product Research. 2014;28(24):2237-2244.
doi:10.1080/14786419.2014.934241 .

Pejin, Boris, Mojović, Miloš, Savić, Aleksandar G, "Novel and highly potent antitumour natural products from cnidarians of marine origin" in Natural Product Research, 28, no. 24 (2014):2237-2244,
https://doi.org/10.1080/14786419.2014.934241 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Kien-Thai, Yong
AU  - Mojović, Miloš
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/756
AB  - The moss Rhodobryum ontariense tea has been claimed by Traditional Chinese medicine for its beneficial effects in the treatment of wide range of cardiovascular diseases including hypertension. The antiradical activity of R. ontariense tea (L e. its lyophilised water extract) was evaluated by electron paramagnetic resonance spectroscopy/against superoxide anion(center dot O-2(-)), nitric-oxide (center dot NO), 2,2-diphenyl-1-picrylhydrazyl (center dot DPPH), carbon-dioxide anion (center dot CO2-) and methoxy (center dot CH2OH) radicals/ and fluorescence spectroscopy/against hydroxyl (center dot OH) radicals/ in in vitro conditions. It reduced the production of majority of radical species tested, but in a varying degree. The most activity was observed against center dot OH, center dot DPPH and center dot CO2- radicals (95 +/- 10%, 78 +/- 4% and 67 +/- 5%, respectively). In addition, the extract was shown to be active against center dot CH2OH and center dot NO radicals (55 +/- 8% and 45 +/- 8%, respectively). On the contrary, no any antiradical activity was observed against center dot O(2)(-)radicals. According to the experimental data obtained, R. ontariense tea can be considered as a promising resource of natural products with antiradical activity and may be of some interest to research in heart disease.
PB  - Adac-Cryptogamie, Paris
T2  - Cryptogamie Bryologie
T1  - Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy
EP  - 179
IS  - 2
SP  - 173
VL  - 35
DO  - 10.7872/cryb.v35.iss2.2014.173
ER  - 

@article{
author = "Pejin, Boris and Savić, Aleksandar G and Kien-Thai, Yong and Mojović, Miloš",
year = "2014",
abstract = "The moss Rhodobryum ontariense tea has been claimed by Traditional Chinese medicine for its beneficial effects in the treatment of wide range of cardiovascular diseases including hypertension. The antiradical activity of R. ontariense tea (L e. its lyophilised water extract) was evaluated by electron paramagnetic resonance spectroscopy/against superoxide anion(center dot O-2(-)), nitric-oxide (center dot NO), 2,2-diphenyl-1-picrylhydrazyl (center dot DPPH), carbon-dioxide anion (center dot CO2-) and methoxy (center dot CH2OH) radicals/ and fluorescence spectroscopy/against hydroxyl (center dot OH) radicals/ in in vitro conditions. It reduced the production of majority of radical species tested, but in a varying degree. The most activity was observed against center dot OH, center dot DPPH and center dot CO2- radicals (95 +/- 10%, 78 +/- 4% and 67 +/- 5%, respectively). In addition, the extract was shown to be active against center dot CH2OH and center dot NO radicals (55 +/- 8% and 45 +/- 8%, respectively). On the contrary, no any antiradical activity was observed against center dot O(2)(-)radicals. According to the experimental data obtained, R. ontariense tea can be considered as a promising resource of natural products with antiradical activity and may be of some interest to research in heart disease.",
publisher = "Adac-Cryptogamie, Paris",
journal = "Cryptogamie Bryologie",
title = "Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy",
pages = "179-173",
number = "2",
volume = "35",
doi = "10.7872/cryb.v35.iss2.2014.173"
}

Pejin, B., Savić, A. G., Kien-Thai, Y.,& Mojović, M.. (2014). Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy. in Cryptogamie Bryologie
Adac-Cryptogamie, Paris., 35(2), 173-179.
https://doi.org/10.7872/cryb.v35.iss2.2014.173

Pejin B, Savić AG, Kien-Thai Y, Mojović M. Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy. in Cryptogamie Bryologie. 2014;35(2):173-179.
doi:10.7872/cryb.v35.iss2.2014.173 .

Pejin, Boris, Savić, Aleksandar G, Kien-Thai, Yong, Mojović, Miloš, "Further in vitro evaluation of antiradical activity of the moss Rhodobryum ontariense tea using EPR and fluorescence spectroscopy" in Cryptogamie Bryologie, 35, no. 2 (2014):173-179,
https://doi.org/10.7872/cryb.v35.iss2.2014.173 . .

TY  - JOUR
AU  - Nikolic, Milos MN
AU  - Marković, Tatjana
AU  - Mojović, Miloš
AU  - Pejin, Boris
AU  - Savić, Aleksandar G
AU  - Peric, Tamara O
AU  - Marković, Dejan Lj
AU  - Stević, Tatjana R
AU  - Soković, Marina
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/675
AB  - The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops and Products
T1  - Chemical composition and biological activity of Gaultheria procumbens L. essential oil
EP  - 567
SP  - 561
VL  - 49
DO  - 10.1016/j.indcrop.2013.06.002
ER  - 

@article{
author = "Nikolic, Milos MN and Marković, Tatjana and Mojović, Miloš and Pejin, Boris and Savić, Aleksandar G and Peric, Tamara O and Marković, Dejan Lj and Stević, Tatjana R and Soković, Marina",
year = "2013",
abstract = "The aim of this study was to examine chemical composition and biological activity of Gaultheria procumbens L essential oil (EO) against food spoilage and oral microorganisms. The components of EO were identified by GC-MS. Antimicrobial activity was determined against food spoilage (five bacteria and six fungal species) and oral microorganisms (eight bacteria and thirty two fungal species) by microdilution and microplate biofilm assay, antioxidant activity was tested using the persistent free radical 2,2-diphenyl-1-picryl hydrazyl (DPPH), while antiradical activity was examined by fluorescence spectroscopy and electron paramagnetic resonance spectroscopy (EPR). GC-MS analysis showed that methyl salicylate (96.90%) was the main component of the oil. Essential oil inhibited the growth of all microorganisms tested, i.e. food and oral bacteria and fungi, respectively (MIC 0.18-3.00 mg/ml and MBC 1.25-4.00 mg/ml; MIC 0.73-5.00 mg/ml and MFC 2.92-26.67 mg/ml); The oil effectively inhibited the biofilm formation of oral Streptococcus mutans and Candida albicans as well (MIC 25.00 MBC 50.00 mg/ml; MIC 12.50, MFC 50.00 mg/ml). In addition, oil exhibited a dose-dependent DPPH-radical-scavenging activity with IC50 value of 30.61 mg/ml. The specific fluorescence probe 2-[6-(4'-amino)phenoxy-3H-xanten-3-on-9-yl] benzoic acid (APP) and the the spin trap 5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO), capable for simultaneous detection of different free radical specie were used in antiradical activity of the oil measurements. Oil showed a moderate antiradical activity, reducing quantity of produced hydroxyl radicals to about 20% of initial value. This study succeeds in creating directly comparable and quantitative data for the oil unsufficiently examined so far.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops and Products",
title = "Chemical composition and biological activity of Gaultheria procumbens L. essential oil",
pages = "567-561",
volume = "49",
doi = "10.1016/j.indcrop.2013.06.002"
}

Nikolic, M. M., Marković, T., Mojović, M., Pejin, B., Savić, A. G., Peric, T. O., Marković, D. L., Stević, T. R.,& Soković, M.. (2013). Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products
Elsevier, Amsterdam., 49, 561-567.
https://doi.org/10.1016/j.indcrop.2013.06.002

Nikolic MM, Marković T, Mojović M, Pejin B, Savić AG, Peric TO, Marković DL, Stević TR, Soković M. Chemical composition and biological activity of Gaultheria procumbens L. essential oil. in Industrial Crops and Products. 2013;49:561-567.
doi:10.1016/j.indcrop.2013.06.002 .

Nikolic, Milos MN, Marković, Tatjana, Mojović, Miloš, Pejin, Boris, Savić, Aleksandar G, Peric, Tamara O, Marković, Dejan Lj, Stević, Tatjana R, Soković, Marina, "Chemical composition and biological activity of Gaultheria procumbens L. essential oil" in Industrial Crops and Products, 49 (2013):561-567,
https://doi.org/10.1016/j.indcrop.2013.06.002 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Jovanović, Katarina K
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/654
AB  - This review covers the 2003-2012 literature data published for antitumor natural products from marine-derived fungi. The focus is on new and highly potent cytotoxic compounds, together with details related to the relevant fungal species. It describes 22 promising bioactives, originating mainly from symbiotic fungi. The chemical structures of all highlighted organic molecules are briefly discussed.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Topics in Medicinal Chemistry
T1  - New and Highly Potent Antitumor Natural Products from Marine-Derived Fungi: Covering the Period from 2003 to 2012
EP  - 2766
IS  - 21
SP  - 2745
VL  - 13
DO  - 10.2174/15680266113136660197
ER  - 

@article{
author = "Pejin, Boris and Jovanović, Katarina K and Mojović, Miloš and Savić, Aleksandar G",
year = "2013",
abstract = "This review covers the 2003-2012 literature data published for antitumor natural products from marine-derived fungi. The focus is on new and highly potent cytotoxic compounds, together with details related to the relevant fungal species. It describes 22 promising bioactives, originating mainly from symbiotic fungi. The chemical structures of all highlighted organic molecules are briefly discussed.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Topics in Medicinal Chemistry",
title = "New and Highly Potent Antitumor Natural Products from Marine-Derived Fungi: Covering the Period from 2003 to 2012",
pages = "2766-2745",
number = "21",
volume = "13",
doi = "10.2174/15680266113136660197"
}

Pejin, B., Jovanović, K. K., Mojović, M.,& Savić, A. G.. (2013). New and Highly Potent Antitumor Natural Products from Marine-Derived Fungi: Covering the Period from 2003 to 2012. in Current Topics in Medicinal Chemistry
Bentham Science Publ Ltd, Sharjah., 13(21), 2745-2766.
https://doi.org/10.2174/15680266113136660197

Pejin B, Jovanović KK, Mojović M, Savić AG. New and Highly Potent Antitumor Natural Products from Marine-Derived Fungi: Covering the Period from 2003 to 2012. in Current Topics in Medicinal Chemistry. 2013;13(21):2745-2766.
doi:10.2174/15680266113136660197 .

Pejin, Boris, Jovanović, Katarina K, Mojović, Miloš, Savić, Aleksandar G, "New and Highly Potent Antitumor Natural Products from Marine-Derived Fungi: Covering the Period from 2003 to 2012" in Current Topics in Medicinal Chemistry, 13, no. 21 (2013):2745-2766,
https://doi.org/10.2174/15680266113136660197 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
AU  - Maksimović, Vuk
AU  - Roemheld, Volker
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/529
AB  - This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.
PB  - Oxford Univ Press, Oxford
T2  - Journal of Experimental Botany
T1  - Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast
EP  - 2420
IS  - 7
SP  - 2411
VL  - 63
DO  - 10.1093/jxb/err359
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Maksimović, Vuk and Roemheld, Volker and Nikolic, Miroslav",
year = "2012",
abstract = "This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.",
publisher = "Oxford Univ Press, Oxford",
journal = "Journal of Experimental Botany",
title = "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast",
pages = "2420-2411",
number = "7",
volume = "63",
doi = "10.1093/jxb/err359"
}

Dragišić Maksimović, J., Mojović, M., Maksimović, V., Roemheld, V.,& Nikolic, M.. (2012). Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany
Oxford Univ Press, Oxford., 63(7), 2411-2420.
https://doi.org/10.1093/jxb/err359

Dragišić Maksimović J, Mojović M, Maksimović V, Roemheld V, Nikolic M. Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany. 2012;63(7):2411-2420.
doi:10.1093/jxb/err359 .

Dragišić Maksimović, Jelena, Mojović, Miloš, Maksimović, Vuk, Roemheld, Volker, Nikolic, Miroslav, "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast" in Journal of Experimental Botany, 63, no. 7 (2012):2411-2420,
https://doi.org/10.1093/jxb/err359 . .