TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
AU  - Maksimović, Vuk
AU  - Roemheld, Volker
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/529
AB  - This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.
PB  - Oxford Univ Press, Oxford
T2  - Journal of Experimental Botany
T1  - Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast
EP  - 2420
IS  - 7
SP  - 2411
VL  - 63
DO  - 10.1093/jxb/err359
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Maksimović, Vuk and Roemheld, Volker and Nikolic, Miroslav",
year = "2012",
abstract = "This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.",
publisher = "Oxford Univ Press, Oxford",
journal = "Journal of Experimental Botany",
title = "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast",
pages = "2420-2411",
number = "7",
volume = "63",
doi = "10.1093/jxb/err359"
}

Dragišić Maksimović, J., Mojović, M., Maksimović, V., Roemheld, V.,& Nikolic, M.. (2012). Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany
Oxford Univ Press, Oxford., 63(7), 2411-2420.
https://doi.org/10.1093/jxb/err359

Dragišić Maksimović J, Mojović M, Maksimović V, Roemheld V, Nikolic M. Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany. 2012;63(7):2411-2420.
doi:10.1093/jxb/err359 .

Dragišić Maksimović, Jelena, Mojović, Miloš, Maksimović, Vuk, Roemheld, Volker, Nikolic, Miroslav, "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast" in Journal of Experimental Botany, 63, no. 7 (2012):2411-2420,
https://doi.org/10.1093/jxb/err359 . .

TY  - JOUR
AU  - Savić, Jasna
AU  - Roemheld, Volker
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/528
AB  - Boron efficiency of 16 oilseed rape genotypes was tested using both hydroponic and pot-soil growing techniques. From the nutrient solution experiment (0.1 and 10 mu M B), 4 representative contrasting genotypes were selected based on relative root and shoot growth. These were then grown in pots with low-B soil (0.25 mg kg(-1)). From the nutrient solution experiment, 2 genotypes selected as B-inefficient did not show any growth disorders, and the concentration of B in the shoots was above critical. Furthermore, 4 contrasting genotypes were subjected to the stable B-11 isotope-enriched uptake solution for 6 h to verify possible low B-induced active uptake by roots and xylem loading of B. The concentration of B-11 in either root cell sap or xylem exudate was higher than in the external nutrient solution, which indicated the presence of low B-induced active uptake for all tested genotypes, and, to some extent, their efficiency with low B. In conclusion, a combination of different growing techniques under controlled environmental conditions together with different parameters including relative root and shoot weight, shoot B concentration, and B uptake provided reliable B efficiency results in oilseed rape genotypes.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Agriculture and Forestry
T1  - Oilseed rape (Brassica napus L.) genotypic variation in response to boron deficiency
EP  - 414
IS  - 4
SP  - 408
VL  - 36
DO  - 10.3906/tar-1109-43
ER  - 

@article{
author = "Savić, Jasna and Roemheld, Volker and Nikolic, Miroslav",
year = "2012",
abstract = "Boron efficiency of 16 oilseed rape genotypes was tested using both hydroponic and pot-soil growing techniques. From the nutrient solution experiment (0.1 and 10 mu M B), 4 representative contrasting genotypes were selected based on relative root and shoot growth. These were then grown in pots with low-B soil (0.25 mg kg(-1)). From the nutrient solution experiment, 2 genotypes selected as B-inefficient did not show any growth disorders, and the concentration of B in the shoots was above critical. Furthermore, 4 contrasting genotypes were subjected to the stable B-11 isotope-enriched uptake solution for 6 h to verify possible low B-induced active uptake by roots and xylem loading of B. The concentration of B-11 in either root cell sap or xylem exudate was higher than in the external nutrient solution, which indicated the presence of low B-induced active uptake for all tested genotypes, and, to some extent, their efficiency with low B. In conclusion, a combination of different growing techniques under controlled environmental conditions together with different parameters including relative root and shoot weight, shoot B concentration, and B uptake provided reliable B efficiency results in oilseed rape genotypes.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Agriculture and Forestry",
title = "Oilseed rape (Brassica napus L.) genotypic variation in response to boron deficiency",
pages = "414-408",
number = "4",
volume = "36",
doi = "10.3906/tar-1109-43"
}

Savić, J., Roemheld, V.,& Nikolic, M.. (2012). Oilseed rape (Brassica napus L.) genotypic variation in response to boron deficiency. in Turkish Journal of Agriculture and Forestry
Tubitak Scientific & Technical Research Council Turkey, Ankara., 36(4), 408-414.
https://doi.org/10.3906/tar-1109-43

Savić J, Roemheld V, Nikolic M. Oilseed rape (Brassica napus L.) genotypic variation in response to boron deficiency. in Turkish Journal of Agriculture and Forestry. 2012;36(4):408-414.
doi:10.3906/tar-1109-43 .

Savić, Jasna, Roemheld, Volker, Nikolic, Miroslav, "Oilseed rape (Brassica napus L.) genotypic variation in response to boron deficiency" in Turkish Journal of Agriculture and Forestry, 36, no. 4 (2012):408-414,
https://doi.org/10.3906/tar-1109-43 . .

TY  - JOUR
AU  - Savić, Jasna
AU  - Nikolic, Miroslav
AU  - Prodanović, Slaven
AU  - Roemheld, Volker
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/189
AB  - The objective of this research was to test the hypothesis of the existence of an active boron ( B) uptake into the cortical cells induced by low B supply. The uptake of B was characterised in two tomato ( Lycopersicon esculentum Mill.) genotypes: B-efficient FER and B-inefficient mutant T3238. In addition, pea (Pisum sativum L.) was used as an anatomically appropriate model for obtaining intact root cortex. Time course uptake studies in tomato indicate that the B-inefficient mutant was defective by the absence of an active low-B-induced uptake system in the cortex. Pea roots showed up to 10-fold higher accumulation of B into the cortex symplast at low (0.5 mu M) external B supply in comparison to adequate B (10 mu M) supply. Also, low-B-induced uptake of B was strongly inhibited by 2,4-dinitrophenol, indicating a metabolic energy-derived active component of B uptake at low external supply. Uptake of B by the cortical cells of tomato and pea plants appears to be a combination of both passive and active components, with a passive component prevailing at higher external B. An active component of B uptake suppressed by either adequate or high B supply might indicate a downregulation of plasma membrane-associated B transporter(s) in root cortical cells.
PB  - Csiro Publishing, Clayton
T2  - Functional Plant Biology
T1  - Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport
EP  - 1136
IS  - 12
SP  - 1130
VL  - 34
DO  - 10.1071/FP07175
ER  - 

@article{
author = "Savić, Jasna and Nikolic, Miroslav and Prodanović, Slaven and Roemheld, Volker",
year = "2007",
abstract = "The objective of this research was to test the hypothesis of the existence of an active boron ( B) uptake into the cortical cells induced by low B supply. The uptake of B was characterised in two tomato ( Lycopersicon esculentum Mill.) genotypes: B-efficient FER and B-inefficient mutant T3238. In addition, pea (Pisum sativum L.) was used as an anatomically appropriate model for obtaining intact root cortex. Time course uptake studies in tomato indicate that the B-inefficient mutant was defective by the absence of an active low-B-induced uptake system in the cortex. Pea roots showed up to 10-fold higher accumulation of B into the cortex symplast at low (0.5 mu M) external B supply in comparison to adequate B (10 mu M) supply. Also, low-B-induced uptake of B was strongly inhibited by 2,4-dinitrophenol, indicating a metabolic energy-derived active component of B uptake at low external supply. Uptake of B by the cortical cells of tomato and pea plants appears to be a combination of both passive and active components, with a passive component prevailing at higher external B. An active component of B uptake suppressed by either adequate or high B supply might indicate a downregulation of plasma membrane-associated B transporter(s) in root cortical cells.",
publisher = "Csiro Publishing, Clayton",
journal = "Functional Plant Biology",
title = "Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport",
pages = "1136-1130",
number = "12",
volume = "34",
doi = "10.1071/FP07175"
}

Savić, J., Nikolic, M., Prodanović, S.,& Roemheld, V.. (2007). Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport. in Functional Plant Biology
Csiro Publishing, Clayton., 34(12), 1130-1136.
https://doi.org/10.1071/FP07175

Savić J, Nikolic M, Prodanović S, Roemheld V. Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport. in Functional Plant Biology. 2007;34(12):1130-1136.
doi:10.1071/FP07175 .

Savić, Jasna, Nikolic, Miroslav, Prodanović, Slaven, Roemheld, Volker, "Boron uptake by the root cortex symplast of tomato and pea plants: evidence for low-boron-induced active transport" in Functional Plant Biology, 34, no. 12 (2007):1130-1136,
https://doi.org/10.1071/FP07175 . .

TY  - JOUR
AU  - Nikolic, Miroslav
AU  - Cesco, Stefano
AU  - Roemheld, Volker
AU  - Varanini, Zeno
AU  - Pinton, Roberto
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/187
AB  - Cucumber (Cucumis sativus L.) plants were precultured for 7 days in either optimal (10 mu M) or low (0.5 mu M) Fe conditions and then grown for further 5 days in a N-free nutrient solution with (+Fe) or without (-Fe) 10 mu M Fe. Thereafter NO(3)(-) (4mM) was added to the nutrient solution for 24 h and, concomitantly, half of the -Fe plants were treated with 1 mu M Fe complexed to water extractable humic substances (WEHS). Supply of NO(3)(-) to + Fe-N-deprived plants caused a large induction in the capacity to take up the anion by roots, which was accompanied by a rise in root-shoot NO(3)(-) concentration. The -Fe plants showed a lower level of induction of NO(3)(-) uptake and hence a lower accumulation of the anion in the tissues, these effects being reversed by supply of Fe-WEHS. Supply of either NO(3)(-) - or NH(4)(+) -N (+/-Fe-WEHS) to -Fe plants promoted the development of the root Fe(III)-chelate reductase activity, but the capacity of roots to take up the Fe(2+) remained unaffected. Results show that an inadequate Fe supply can limit the acquisition of NO(3)(-), whereas NO(3)(-) supply can affect Fe uptake by influencing the development and maintenance of a high Fe(III)-chelate reducing capacity.
PB  - Csiro Publishing, Collingwood
T2  - Functional Plant Biology
T1  - Short-term interactions between nitrate and iron nutrition in cucumber
EP  - 408
IS  - 5
SP  - 402
VL  - 34
DO  - 10.1071/FP07022
ER  - 

@article{
author = "Nikolic, Miroslav and Cesco, Stefano and Roemheld, Volker and Varanini, Zeno and Pinton, Roberto",
year = "2007",
abstract = "Cucumber (Cucumis sativus L.) plants were precultured for 7 days in either optimal (10 mu M) or low (0.5 mu M) Fe conditions and then grown for further 5 days in a N-free nutrient solution with (+Fe) or without (-Fe) 10 mu M Fe. Thereafter NO(3)(-) (4mM) was added to the nutrient solution for 24 h and, concomitantly, half of the -Fe plants were treated with 1 mu M Fe complexed to water extractable humic substances (WEHS). Supply of NO(3)(-) to + Fe-N-deprived plants caused a large induction in the capacity to take up the anion by roots, which was accompanied by a rise in root-shoot NO(3)(-) concentration. The -Fe plants showed a lower level of induction of NO(3)(-) uptake and hence a lower accumulation of the anion in the tissues, these effects being reversed by supply of Fe-WEHS. Supply of either NO(3)(-) - or NH(4)(+) -N (+/-Fe-WEHS) to -Fe plants promoted the development of the root Fe(III)-chelate reductase activity, but the capacity of roots to take up the Fe(2+) remained unaffected. Results show that an inadequate Fe supply can limit the acquisition of NO(3)(-), whereas NO(3)(-) supply can affect Fe uptake by influencing the development and maintenance of a high Fe(III)-chelate reducing capacity.",
publisher = "Csiro Publishing, Collingwood",
journal = "Functional Plant Biology",
title = "Short-term interactions between nitrate and iron nutrition in cucumber",
pages = "408-402",
number = "5",
volume = "34",
doi = "10.1071/FP07022"
}

Nikolic, M., Cesco, S., Roemheld, V., Varanini, Z.,& Pinton, R.. (2007). Short-term interactions between nitrate and iron nutrition in cucumber. in Functional Plant Biology
Csiro Publishing, Collingwood., 34(5), 402-408.
https://doi.org/10.1071/FP07022

Nikolic M, Cesco S, Roemheld V, Varanini Z, Pinton R. Short-term interactions between nitrate and iron nutrition in cucumber. in Functional Plant Biology. 2007;34(5):402-408.
doi:10.1071/FP07022 .

Nikolic, Miroslav, Cesco, Stefano, Roemheld, Volker, Varanini, Zeno, Pinton, Roberto, "Short-term interactions between nitrate and iron nutrition in cucumber" in Functional Plant Biology, 34, no. 5 (2007):402-408,
https://doi.org/10.1071/FP07022 . .