Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast
Само за регистроване кориснике
2012
Аутори
Dragišić Maksimović, JelenaMojović, Miloš
Maksimović, Vuk
Roemheld, Volker
Nikolic, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although s...upplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.
Кључне речи:
silicon / peroxidase / manganese toxicity / leaves / hydroxyl radicals / cucumber (Cucumis sativus L / cell walls / Apoplastic fluidИзвор:
Journal of Experimental Botany, 2012, 63, 7, 2411-2420Издавач:
- Oxford Univ Press, Oxford
Финансирање / пројекти:
- Минерални стрес и адаптације биљака на маргиналним пољопривредним земљиштима (RS-MESTD-Basic Research (BR or ON)-173028)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1093/jxb/err359
ISSN: 0022-0957
PubMed: 22249995
WoS: 000304052300003
Scopus: 2-s2.0-84861018535
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Dragišić Maksimović, Jelena AU - Mojović, Miloš AU - Maksimović, Vuk AU - Roemheld, Volker AU - Nikolic, Miroslav PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/529 AB - This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH. PB - Oxford Univ Press, Oxford T2 - Journal of Experimental Botany T1 - Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast EP - 2420 IS - 7 SP - 2411 VL - 63 DO - 10.1093/jxb/err359 ER -
@article{ author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Maksimović, Vuk and Roemheld, Volker and Nikolic, Miroslav", year = "2012", abstract = "This work was focused on the role of silicon (Si) in amelioration of manganese (Mn) toxicity caused by elevated production of hydroxyl radicals (center dot OH) in the leaf apoplast of cucumber (Cucumis sativus L.). The plants were grown in nutrient solutions with adequate (0.5 mu M) or excessive (100 mu M) Mn concentrations with or without Si being supplied. The symptoms of Mn toxicity were absent in the leaves of Si-treated plants subjected to excess Mn, although the leaf Mn concentration remained extremely high. The apoplastic concentration of free Mn2+ and H2O2 of high Mn-treated plants was significantly decreased by Si treatment. Si supply suppressed the Mn-induced increased abundance of peroxidase (POD) isoforms in the leaf apoplastic fluid, and led to a rapid suppression of guaiacol-POD activity under excess Mn. The spin-trapping reagent 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide was used to detect center dot OH by electron paramagnetic resonance spectroscopy. Although supplying Si markedly decreased the accumulation of center dot OH in the leaf apoplast with excess Mn, adding monosilicic acid to the Mn2+/H2O2 reaction mixture did not directly affect the Fenton reaction in vitro. The results indicate that Si contributes indirectly to a decrease in center dot OH in the leaf apoplast by decreasing the free apoplastic Mn2+, thus regulating the Fenton reaction. A direct inhibitory effect of Si on guaiacol-POD activity (demonstrated in vitro) may also contribute to decreasing the POD-mediated generation of center dot OH.", publisher = "Oxford Univ Press, Oxford", journal = "Journal of Experimental Botany", title = "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast", pages = "2420-2411", number = "7", volume = "63", doi = "10.1093/jxb/err359" }
Dragišić Maksimović, J., Mojović, M., Maksimović, V., Roemheld, V.,& Nikolic, M.. (2012). Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany Oxford Univ Press, Oxford., 63(7), 2411-2420. https://doi.org/10.1093/jxb/err359
Dragišić Maksimović J, Mojović M, Maksimović V, Roemheld V, Nikolic M. Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast. in Journal of Experimental Botany. 2012;63(7):2411-2420. doi:10.1093/jxb/err359 .
Dragišić Maksimović, Jelena, Mojović, Miloš, Maksimović, Vuk, Roemheld, Volker, Nikolic, Miroslav, "Silicon ameliorates manganese toxicity in cucumber by decreasing hydroxyl radical accumulation in the leaf apoplast" in Journal of Experimental Botany, 63, no. 7 (2012):2411-2420, https://doi.org/10.1093/jxb/err359 . .