Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging
Апстракт
Cereal seeds are susceptible to infection by the opportunistic pathogen Aspergillus flavus that
consequently leads to the contamination with aflatoxins. In this work, EPR imaging was applied
for mapping redox state of the maize and wheat seeds contaminated with aflatoxin, through detection
and localization of paramagnetic spin probes in vivo. The sample recordings were made
in the L-frequency domain (1.1 GHz). The 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl
(3-Carbamoyl-PROXYL) spin probe was used for the estimation of changes in the redox state of the
aflatoxin-stressed seeds. The EPR-active probes were reduced by cellular reducing agents forming
EPR inactive species (hydroxylamines). The reduction rate, which among other factors depends
on the redox status of the cell, provides information about the redox environment in the region
of interest. A higher and more localized 3-Carbamoyl-PROXYL spin-active probe signal has been
found in uncontaminated seeds than in afl...atoxin-stressed seeds. Our results imply that aflatoxin
contamination leads to the change in the seeds’ redox status which provides useful information
about its impact on seed metabolism.
Кључне речи:
aflatoxins / seeds / redox status / electron paramagnetic resonance imagingИзвор:
3rd International Conference on Plant Biology 22nd SPPS, Belgrade, 2018, 61-61Издавач:
- Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Bartolić, Dragana AU - Stanković, Mira AU - Mojović, Miloš AU - Maksimović, Vuk AU - Radotić, Ksenija PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2795 AB - Cereal seeds are susceptible to infection by the opportunistic pathogen Aspergillus flavus that consequently leads to the contamination with aflatoxins. In this work, EPR imaging was applied for mapping redox state of the maize and wheat seeds contaminated with aflatoxin, through detection and localization of paramagnetic spin probes in vivo. The sample recordings were made in the L-frequency domain (1.1 GHz). The 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl (3-Carbamoyl-PROXYL) spin probe was used for the estimation of changes in the redox state of the aflatoxin-stressed seeds. The EPR-active probes were reduced by cellular reducing agents forming EPR inactive species (hydroxylamines). The reduction rate, which among other factors depends on the redox status of the cell, provides information about the redox environment in the region of interest. A higher and more localized 3-Carbamoyl-PROXYL spin-active probe signal has been found in uncontaminated seeds than in aflatoxin-stressed seeds. Our results imply that aflatoxin contamination leads to the change in the seeds’ redox status which provides useful information about its impact on seed metabolism. PB - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade C3 - 3rd International Conference on Plant Biology 22nd SPPS, Belgrade T1 - Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging EP - 61 SP - 61 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2795 ER -
@conference{ author = "Bartolić, Dragana and Stanković, Mira and Mojović, Miloš and Maksimović, Vuk and Radotić, Ksenija", year = "2018", abstract = "Cereal seeds are susceptible to infection by the opportunistic pathogen Aspergillus flavus that consequently leads to the contamination with aflatoxins. In this work, EPR imaging was applied for mapping redox state of the maize and wheat seeds contaminated with aflatoxin, through detection and localization of paramagnetic spin probes in vivo. The sample recordings were made in the L-frequency domain (1.1 GHz). The 3-Carbamoyl-2,2,5,5-tetramethyl-1-pyrrolidinyl-N-oxyl (3-Carbamoyl-PROXYL) spin probe was used for the estimation of changes in the redox state of the aflatoxin-stressed seeds. The EPR-active probes were reduced by cellular reducing agents forming EPR inactive species (hydroxylamines). The reduction rate, which among other factors depends on the redox status of the cell, provides information about the redox environment in the region of interest. A higher and more localized 3-Carbamoyl-PROXYL spin-active probe signal has been found in uncontaminated seeds than in aflatoxin-stressed seeds. Our results imply that aflatoxin contamination leads to the change in the seeds’ redox status which provides useful information about its impact on seed metabolism.", publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade", journal = "3rd International Conference on Plant Biology 22nd SPPS, Belgrade", title = "Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging", pages = "61-61", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2795" }
Bartolić, D., Stanković, M., Mojović, M., Maksimović, V.,& Radotić, K.. (2018). Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging. in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković”, University of Belgrade Faculty of Biology, University of Belgrade., 61-61. https://hdl.handle.net/21.15107/rcub_rimsi_2795
Bartolić D, Stanković M, Mojović M, Maksimović V, Radotić K. Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging. in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade. 2018;:61-61. https://hdl.handle.net/21.15107/rcub_rimsi_2795 .
Bartolić, Dragana, Stanković, Mira, Mojović, Miloš, Maksimović, Vuk, Radotić, Ksenija, "Non-invasive mapping of redox status in the aflatoxin-stressed maize and wheat seeds by 2D electron paramagnetic resonance imaging" in 3rd International Conference on Plant Biology 22nd SPPS, Belgrade (2018):61-61, https://hdl.handle.net/21.15107/rcub_rimsi_2795 .