TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Kovačević, Snežana
AU  - Jovanović, Uroš
AU  - Stanić, Marina
AU  - Opačić, Miloš
AU  - Santrač, Isidora
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2421
AB  - Heavy metals remain a major pollutant in waters near mining sites. Water pollution is a current, longterm problem that affects plants and organisms that live in these water systems, and the effect is very harmful not only for individual species and populations, but also for the entire biological community. This study analyzed the potential of the microalga Chlorella sorokiniana in the adsorption of four selected metals: Fe, Cu, Zn and Ni, all present in high amounts in Lake Robule. This pilot study was conducted to evaluate the potential of these microalgae for possible use in future bioremediation treatment of these water. A laboratory study of metal accumulation in lake water samples lasted for 7 days, after which a total decrease in metal concentration was observed, namely Fe ~25%, Cu ~17%, and Zn ~4% on the seventh day. This study confirms the strong potential of microalgae C. sorokiniana to reduce the presence of heavy metals in conditions known for the low pH value of water with a high percentage of Fe and other heavy metals.
PB  - University of Belgrade
PB  - Technical Faculty in Bor
C3  - 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia
T1  - Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule
EP  - 334
SP  - 330
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2421
ER  - 

@conference{
author = "Dimitrijević, Milena and Kovačević, Snežana and Jovanović, Uroš and Stanić, Marina and Opačić, Miloš and Santrač, Isidora and Tanović, Marija and Ćurić, Valentina and Spasojević, Ivan",
year = "2023",
abstract = "Heavy metals remain a major pollutant in waters near mining sites. Water pollution is a current, longterm problem that affects plants and organisms that live in these water systems, and the effect is very harmful not only for individual species and populations, but also for the entire biological community. This study analyzed the potential of the microalga Chlorella sorokiniana in the adsorption of four selected metals: Fe, Cu, Zn and Ni, all present in high amounts in Lake Robule. This pilot study was conducted to evaluate the potential of these microalgae for possible use in future bioremediation treatment of these water. A laboratory study of metal accumulation in lake water samples lasted for 7 days, after which a total decrease in metal concentration was observed, namely Fe ~25%, Cu ~17%, and Zn ~4% on the seventh day. This study confirms the strong potential of microalgae C. sorokiniana to reduce the presence of heavy metals in conditions known for the low pH value of water with a high percentage of Fe and other heavy metals.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia",
title = "Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule",
pages = "334-330",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2421"
}

Dimitrijević, M., Kovačević, S., Jovanović, U., Stanić, M., Opačić, M., Santrač, I., Tanović, M., Ćurić, V.,& Spasojević, I.. (2023). Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule. in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia
University of Belgrade., 330-334.
https://hdl.handle.net/21.15107/rcub_rimsi_2421

Dimitrijević M, Kovačević S, Jovanović U, Stanić M, Opačić M, Santrač I, Tanović M, Ćurić V, Spasojević I. Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule. in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia. 2023;:330-334.
https://hdl.handle.net/21.15107/rcub_rimsi_2421 .

Dimitrijević, Milena, Kovačević, Snežana, Jovanović, Uroš, Stanić, Marina, Opačić, Miloš, Santrač, Isidora, Tanović, Marija, Ćurić, Valentina, Spasojević, Ivan, "Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule" in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia (2023):330-334,
https://hdl.handle.net/21.15107/rcub_rimsi_2421 .

TY  - CONF
AU  - Ćukić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Kovačević, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2419
AB  - Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana
EP  - 230
SP  - 230
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2419
ER  - 

@conference{
author = "Ćukić, Aleksandra and Dimitrijević, Milena and Kovačević, Snežana and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2022",
abstract = "Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana",
pages = "230-230",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2419"
}

Ćukić, A., Dimitrijević, M., Kovačević, S., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2022). Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419

Ćukić A, Dimitrijević M, Kovačević S, Opačić M, Stanić M, Žižić M, Spasojević I. Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

Ćukić, Aleksandra, Dimitrijević, Milena, Kovačević, Snežana, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):230-230,
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Danilović Luković, Jelena
AU  - Milivojević, Marija
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2403
AB  - METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana
EP  - 748
SP  - 747
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2403
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Danilović Luković, Jelena and Milivojević, Marija and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana",
pages = "748-747",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2403"
}

Vojvodić, S., Stanić, M., Danilović Luković, J., Milivojević, M., Zechmann, B., Dimitrijević, M., Opačić, M., Pittman, J. K.,& Spasojević, I.. (2022). Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403

Vojvodić S, Stanić M, Danilović Luković J, Milivojević M, Zechmann B, Dimitrijević M, Opačić M, Pittman JK, Spasojević I. Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

Vojvodić, Snežana, Stanić, Marina, Danilović Luković, Jelena, Milivojević, Marija, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Pittman, Jon K., Spasojević, Ivan, "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):747-748,
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

TY  - THES
AU  - Opačić, Miloš
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1859
AB  - Epilepsija mezijalno-temporalnog režnja (mTLE) asocirana sa hipokampusnom sklerozom (HS) je najčešći epileptični sindrom. HS se ogleda u značajnom gubitku neurona hipokampusa. Budući da je HS praćena smanjenim nivoom bakra (Cu), cilj ove disertacije je bio otkriti ulogu ovih promena u patogenezi HS kroz ispitivanje veze narušene koncentracije Cu i gubitka neurona, kao i glavnih puteva unosa, transporta i iskorišćavanja Cu u mitohondrijama u kontekstu energetskog metabolizma. Ispitivanja su izvršena na humanim uzorcima hipokampusa, a primenjene su analitičke i metode bio-oslikavanja: laserska ablacija uz induktivno spregnutu plazmu i masenu spektrometriju za oslikavanje metala, in situ hibridizacija za ispitivanje ekspresije iRNK, histohemijske metode za praćenje neurodege-neracije i ispitivanje tkivne ekspresije ciljnih proteina, kao i metoda imunoblota za ispitivanje zastupljenosti proteina od interesa u tkivnim lizatima.Rezultati izneseni u ovoj disertaciji pokazali su da kod humanih sklerotičnih hipokampusa postoje patološke promene u koncentraciji Cu koje koreliraju sa propadanjem neurona. Nivo membranskog transportera za Cu, SLC31A1 je bio povećan u izraženoj sklerozi a smanjen u ograničenoj i lokalizovanoj sklerozi, u odnosu na kontrolno tkivo. Smanjeni nivoi iRNK šaperona za transport i umetanje Cu u aktivna mesta enzimskog kompleksa citohom c oksidaze (COX) kao i smanjena aktivnost COX u odnosu na kontrolne uzorke ukazuju na promene u mitohondrijskom transportu Cu. Ustanovljena je pozitivna korelacija između distribucije Cu, brojnosti piramidalnih neurona i aktivnosti COX u sklerotičnim zonama. Rezultati ove disertacije čine korak napred u razumevanju patologije mTLE-HS i predstavljaju osnov za unapređenje tretmana bolesti i istraživanja na polju novih terapeutika čija bi glavna meta bila narušena homeostaza Cu.
AB  - Mesial temporal lobe epilepsy (mTLE) associated with hippocampal sclerosis (HS) is the single most prevalent epilepsy syndrome. HS is characterized by substantial loss of hippocampal neurons. Regarding diminished copper (Cu) levels detected in HS, the aim of this dissertation was to discover the role of these changes HS pathology by examining the relationship between altered Cu level and neuronal loss, through the main roads of cellular Cu intake and transport and its utilization in mitochondria, in the terms of energy metabolism. Research has been conducted on human hippocampal samples, applying a battery of techniques: laser ablation inductively coupled plasma mass spectrometry for elemental imaging, in situ hybridization for mRNA expression analysis, histochemical methods for investigation of protein expression in tissue and neurodegeneration evaluation, and immunoblotting for examination of specific proteins in tissue homogenate.Results presented herein implied the presence of pathological changes in Cu concentrations in correlation with neuronal deterioration in the human sclerotic hippocampi. The level of membrane Cu importer SLC31A1 was elevated in widespread sclerosis but decreased in less extensive and localized one, compared to controls. Decreased levels of cytochrome c oxidase (COX) copper chaperones’ mRNA and reduced COX activity in comparison to controls revealed altered mitochondrial Cu transport. Positive correlation was established for Cu distribution, neuronal count and COX activity in sclerotic regions of the hippocampi. Findings in this dissertation are a step forward towards better understanding mTLE-HS pathology and represent a starting point for the improvement of the disease treatment and development of novel noninvasive therapy approaches which would tackle the impaired copper homeostasis.
PB  - Универзитет у Београду, Биолошки факултет
T2  - Универзитет у Београду
T1  - Uloga i metabolizam bakra u hipokampusnoj sklerozi asociranoj sa epilepsijom temporalnog režnja kod čoveka
UR  - https://hdl.handle.net/21.15107/rcub_nardus_20685
ER  - 

@phdthesis{
author = "Opačić, Miloš",
year = "2022",
abstract = "Epilepsija mezijalno-temporalnog režnja (mTLE) asocirana sa hipokampusnom sklerozom (HS) je najčešći epileptični sindrom. HS se ogleda u značajnom gubitku neurona hipokampusa. Budući da je HS praćena smanjenim nivoom bakra (Cu), cilj ove disertacije je bio otkriti ulogu ovih promena u patogenezi HS kroz ispitivanje veze narušene koncentracije Cu i gubitka neurona, kao i glavnih puteva unosa, transporta i iskorišćavanja Cu u mitohondrijama u kontekstu energetskog metabolizma. Ispitivanja su izvršena na humanim uzorcima hipokampusa, a primenjene su analitičke i metode bio-oslikavanja: laserska ablacija uz induktivno spregnutu plazmu i masenu spektrometriju za oslikavanje metala, in situ hibridizacija za ispitivanje ekspresije iRNK, histohemijske metode za praćenje neurodege-neracije i ispitivanje tkivne ekspresije ciljnih proteina, kao i metoda imunoblota za ispitivanje zastupljenosti proteina od interesa u tkivnim lizatima.Rezultati izneseni u ovoj disertaciji pokazali su da kod humanih sklerotičnih hipokampusa postoje patološke promene u koncentraciji Cu koje koreliraju sa propadanjem neurona. Nivo membranskog transportera za Cu, SLC31A1 je bio povećan u izraženoj sklerozi a smanjen u ograničenoj i lokalizovanoj sklerozi, u odnosu na kontrolno tkivo. Smanjeni nivoi iRNK šaperona za transport i umetanje Cu u aktivna mesta enzimskog kompleksa citohom c oksidaze (COX) kao i smanjena aktivnost COX u odnosu na kontrolne uzorke ukazuju na promene u mitohondrijskom transportu Cu. Ustanovljena je pozitivna korelacija između distribucije Cu, brojnosti piramidalnih neurona i aktivnosti COX u sklerotičnim zonama. Rezultati ove disertacije čine korak napred u razumevanju patologije mTLE-HS i predstavljaju osnov za unapređenje tretmana bolesti i istraživanja na polju novih terapeutika čija bi glavna meta bila narušena homeostaza Cu., Mesial temporal lobe epilepsy (mTLE) associated with hippocampal sclerosis (HS) is the single most prevalent epilepsy syndrome. HS is characterized by substantial loss of hippocampal neurons. Regarding diminished copper (Cu) levels detected in HS, the aim of this dissertation was to discover the role of these changes HS pathology by examining the relationship between altered Cu level and neuronal loss, through the main roads of cellular Cu intake and transport and its utilization in mitochondria, in the terms of energy metabolism. Research has been conducted on human hippocampal samples, applying a battery of techniques: laser ablation inductively coupled plasma mass spectrometry for elemental imaging, in situ hybridization for mRNA expression analysis, histochemical methods for investigation of protein expression in tissue and neurodegeneration evaluation, and immunoblotting for examination of specific proteins in tissue homogenate.Results presented herein implied the presence of pathological changes in Cu concentrations in correlation with neuronal deterioration in the human sclerotic hippocampi. The level of membrane Cu importer SLC31A1 was elevated in widespread sclerosis but decreased in less extensive and localized one, compared to controls. Decreased levels of cytochrome c oxidase (COX) copper chaperones’ mRNA and reduced COX activity in comparison to controls revealed altered mitochondrial Cu transport. Positive correlation was established for Cu distribution, neuronal count and COX activity in sclerotic regions of the hippocampi. Findings in this dissertation are a step forward towards better understanding mTLE-HS pathology and represent a starting point for the improvement of the disease treatment and development of novel noninvasive therapy approaches which would tackle the impaired copper homeostasis.",
publisher = "Универзитет у Београду, Биолошки факултет",
journal = "Универзитет у Београду",
title = "Uloga i metabolizam bakra u hipokampusnoj sklerozi asociranoj sa epilepsijom temporalnog režnja kod čoveka",
url = "https://hdl.handle.net/21.15107/rcub_nardus_20685"
}

Opačić, M.. (2022). Uloga i metabolizam bakra u hipokampusnoj sklerozi asociranoj sa epilepsijom temporalnog režnja kod čoveka. in Универзитет у Београду
Универзитет у Београду, Биолошки факултет..
https://hdl.handle.net/21.15107/rcub_nardus_20685

Opačić M. Uloga i metabolizam bakra u hipokampusnoj sklerozi asociranoj sa epilepsijom temporalnog režnja kod čoveka. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_20685 .

Opačić, Miloš, "Uloga i metabolizam bakra u hipokampusnoj sklerozi asociranoj sa epilepsijom temporalnog režnja kod čoveka" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_nardus_20685 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Dimitrijević, Milena
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2417
AB  - Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
T1  - Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2417
ER  - 

@conference{
author = "Vojvodić, Snežana and Opačić, Miloš and Stanić, Marina and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia",
title = "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2417"
}

Vojvodić, S., Opačić, M., Stanić, M., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2022). Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417

Vojvodić S, Opačić M, Stanić M, Dimitrijević M, Pittman JK, Spasojević I. Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia. 2022;:133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

Vojvodić, Snežana, Opačić, Miloš, Stanić, Marina, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess" in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia (2022):133-133,
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Djordjevic, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1570
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
SP  - 9023
VL  - 23
DO  - 10.3390/ijms23169023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović Milutinović, Danijela and Blagojević, Duško and Djordjevic, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
pages = "9023",
volume = "23",
doi = "10.3390/ijms23169023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović Milutinović, D., Blagojević, D., Djordjevic, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović Milutinović D, Blagojević D, Djordjevic A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović Milutinović, Danijela, Blagojević, Duško, Djordjevic, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Dučić, Tanja
AU  - Stanković, Dalibor
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2391
AB  - Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)
EP  - 174
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2391
ER  - 

@conference{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Dučić, Tanja and Stanković, Dalibor and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2391"
}

Vojvodić, S., Dimitrijević, M., Dučić, T., Stanković, D., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2021). Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391

Vojvodić S, Dimitrijević M, Dučić T, Stanković D, Opačić M, Stanić M, Žižić M, Spasojević I. Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

Vojvodić, Snežana, Dimitrijević, Milena, Dučić, Tanja, Stanković, Dalibor, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):174-174,
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2420
AB  - Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.
PB  - Elsevier
PB  - Society for Free Radical Research Europe (SFRR-E)
PB  - Ministry of Education, Science and Technological Development (Republic of Serbia)
PB  - Oxygen Club of California
C3  - Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
T1  - Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana
EP  - 145
SP  - 145
DO  - 10.1016/j.freeradbiomed.2021.08.148
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Danilović Luković, Jelena and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2021",
abstract = "Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.",
publisher = "Elsevier, Society for Free Radical Research Europe (SFRR-E), Ministry of Education, Science and Technological Development (Republic of Serbia), Oxygen Club of California",
journal = "Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts",
title = "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana",
pages = "145-145",
doi = "10.1016/j.freeradbiomed.2021.08.148"
}

Vojvodić, S., Stanić, M., Zechmann, B., Dimitrijević, M., Opačić, M., Danilović Luković, J., Morina, A., Pittman, J. K.,& Spasojević, I.. (2021). Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
Elsevier., 145-145.
https://doi.org/10.1016/j.freeradbiomed.2021.08.148

Vojvodić S, Stanić M, Zechmann B, Dimitrijević M, Opačić M, Danilović Luković J, Morina A, Pittman JK, Spasojević I. Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts. 2021;:145-145.
doi:10.1016/j.freeradbiomed.2021.08.148 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Danilović Luković, Jelena, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana" in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts (2021):145-145,
https://doi.org/10.1016/j.freeradbiomed.2021.08.148 . .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Milić, Jelena
AU  - Stanić, Marina
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2392
AB  - Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - The effect of increased nickel concentrations on Chlorella sorokiniana culture
EP  - 58
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2392
ER  - 

@conference{
author = "Dimitrijević, Milena and Vojvodić, Snežana and Opačić, Miloš and Danilović Luković, Jelena and Milić, Jelena and Stanić, Marina and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "The effect of increased nickel concentrations on Chlorella sorokiniana culture",
pages = "58-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2392"
}

Dimitrijević, M., Vojvodić, S., Opačić, M., Danilović Luković, J., Milić, J., Stanić, M.,& Spasojević, I.. (2021). The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392

Dimitrijević M, Vojvodić S, Opačić M, Danilović Luković J, Milić J, Stanić M, Spasojević I. The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

Dimitrijević, Milena, Vojvodić, Snežana, Opačić, Miloš, Danilović Luković, Jelena, Milić, Jelena, Stanić, Marina, Spasojević, Ivan, "The effect of increased nickel concentrations on Chlorella sorokiniana culture" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):57-58,
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Raicević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Zivin, Marko
AU  - Selih, Vid Simon
AU  - Spasojević, Ivan
AU  - Savić, Danijela Z
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1515
AB  - Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.
PB  - Wiley, Hoboken
T2  - Brain and Behavior
T1  - Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients
IS  - 2
VL  - 11
DO  - 10.1002/brb3.1986
ER  - 

@article{
author = "Opačić, Miloš and Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Raicević, Savo and Savić, Slobodan and Zorović, Maja and Zivin, Marko and Selih, Vid Simon and Spasojević, Ivan and Savić, Danijela Z",
year = "2021",
abstract = "Introduction Disruption of copper homeostasis and dysfunction of mitochondria have been documented in sclerotic hippocampi (HS) of patients with mesial temporal lobe epilepsy (mTLE). However, a potential link between these pathological changes has not been tackled so far. Herein, we analyzed regional distribution of neuron somata density, copper concentration, and the activity of cytochrome c oxidase (CCO), a component of mitochondrial electron transport chain and copper-containing metalloprotein, in HS. Methods Histochemical staining and laser ablation inductively coupled plasma mass spectrometry were carried out to construct comparable maps of these parameters in coronal sections of hippocampi of 3 mTLE-HS patients and 3 control subjects. Results Copper levels were decreased in all regions of HS with pyramidal neuron somata. CCO activity was significantly reduced in stratum pyramidale (PY) 1 and cornu Ammonis field 4, the two regions with significant reduction in neuron somata density. CCO activity was also lower in layers that contain apical dendrites of pyramidal neurons and mossy fibers. It appears that copper deficiency in PY2 and PY3 comes before CCO activity reduction and neuronal loss. A strong positive correlation was found between neuron density, Cu concentration, and CCO activity. Conclusions Presented results imply that pathological alterations in Cu and energy metabolism could be involved in the development of HS. A limitation of this study was the relatively small number of patients. However, presented results underline copper deficiency as a component of pathological mechanisms of epilepsy and warrant further investigation of cuproproteins and members of copper transport machinery.",
publisher = "Wiley, Hoboken",
journal = "Brain and Behavior",
title = "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients",
number = "2",
volume = "11",
doi = "10.1002/brb3.1986"
}

Opačić, M., Ristic, A. J., Sokic, D., Baščarević, V., Raicević, S., Savić, S., Zorović, M., Zivin, M., Selih, V. S., Spasojević, I.,& Savić, D. Z.. (2021). Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior
Wiley, Hoboken., 11(2).
https://doi.org/10.1002/brb3.1986

Opačić M, Ristic AJ, Sokic D, Baščarević V, Raicević S, Savić S, Zorović M, Zivin M, Selih VS, Spasojević I, Savić DZ. Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients. in Brain and Behavior. 2021;11(2).
doi:10.1002/brb3.1986 .

Opačić, Miloš, Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Raicević, Savo, Savić, Slobodan, Zorović, Maja, Zivin, Marko, Selih, Vid Simon, Spasojević, Ivan, Savić, Danijela Z, "Regional distribution of cytochrome c oxidase activity and copper in sclerotic hippocampi of epilepsy patients" in Brain and Behavior, 11, no. 2 (2021),
https://doi.org/10.1002/brb3.1986 . .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana M.
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Savić, Danijela Z
AU  - Grguric-Sipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1344
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O-2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors
EP  - 127
SP  - 123
VL  - 148
DO  - 10.1016/j.freeradbiomed.2020.01.001
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana M. and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena and Savić, Danijela Z and Grguric-Sipka, Sanja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O-2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors",
pages = "127-123",
volume = "148",
doi = "10.1016/j.freeradbiomed.2020.01.001"
}

Korać Jačić, J., Nikolić, L. M., Stanković, D. M., Opačić, M., Dimitrijević, M., Savić, D. Z., Grguric-Sipka, S., Spasojević, I.,& Bogdanović Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić LM, Stanković DM, Opačić M, Dimitrijević M, Savić DZ, Grguric-Sipka S, Spasojević I, Bogdanović Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana M., Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena, Savić, Danijela Z, Grguric-Sipka, Sanja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors" in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Opačić, Miloš
AU  - Bajčetić, Milica
AU  - Mandic, Vesna
AU  - Maglic, Dragana
AU  - Miković, Zeljko
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1316
AB  - Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1(st)and the 2(nd)trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3(rd)trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias
IS  - 6
VL  - 15
DO  - 10.1371/journal.pone.0234253
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Opačić, Miloš and Bajčetić, Milica and Mandic, Vesna and Maglic, Dragana and Miković, Zeljko and Spasojević, Ivan",
year = "2020",
abstract = "Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1(st)and the 2(nd)trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3(rd)trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias",
number = "6",
volume = "15",
doi = "10.1371/journal.pone.0234253"
}

Bogdanović Pristov, J., Opačić, M., Bajčetić, M., Mandic, V., Maglic, D., Miković, Z.,& Spasojević, I.. (2020). Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias. in PLoS One
Public Library Science, San Francisco., 15(6).
https://doi.org/10.1371/journal.pone.0234253

Bogdanović Pristov J, Opačić M, Bajčetić M, Mandic V, Maglic D, Miković Z, Spasojević I. Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias. in PLoS One. 2020;15(6).
doi:10.1371/journal.pone.0234253 .

Bogdanović Pristov, Jelena, Opačić, Miloš, Bajčetić, Milica, Mandic, Vesna, Maglic, Dragana, Miković, Zeljko, Spasojević, Ivan, "Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias" in PLoS One, 15, no. 6 (2020),
https://doi.org/10.1371/journal.pone.0234253 . .

TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Zorović, Maja
AU  - Živin, Marko
AU  - Selih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2841
AB  - More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.
PB  - FENS
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The importance of copper in pathology of mesial temporal lobe epilepsy
SP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2841
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Nestorov, Jelena and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Zorović, Maja and Živin, Marko and Selih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2019",
abstract = "More and more studies are identifying the regulation of metal homeostasis as one of the key points of central nervous system’s well-being. Epilepsy is a particularly interesting neurological condition when viewed in terms of the correlation between the amount of metals and the development of a seizure. This lecture will present contribution of our group to the field of metal biology in epilepsy by mapping brain metals in sclerotic hippocampus resected from drug resistant mesial temporal lobe epilepsy (mTLE) patients as surgical therapeutic approach. Direct insight into this epileptogenic area, by two powerful techniques, optical emission and mass spectrometry, has led us to investigation of copper turnover. Namely, among the examined metals, we found the deficiency of copper in sclerotic hippocampus on two levels: (i) in whole structure (ii) and locally in the areas of neuronal loss, with significant correlation between copper concentration and neuron density. Furthermore, analysis of copper metalloproteins showed: (i) significant increase or decrease in levels of protein that is participating in copper transport into the cell (CTR1) depending on the degree of hippocampal neuronal loss; (ii) and lower activity of an enzyme in which copper is part of the active site, cytochrome c oxidase, in sclerotic hippocampi of patients compared to control tissue. In our further investigations it remained to be determined whether changes in copper concentrations and copper metalloproteins are causal to pathology of mTLE or they represent epiphenomenon.",
publisher = "FENS",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The importance of copper in pathology of mesial temporal lobe epilepsy",
pages = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2841"
}

Savic, D., Opačić, M., Nestorov, J., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Zorović, M., Živin, M., Selih, V. S., Spasic, S.,& Spasojević, I.. (2019). The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
FENS., 46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841

Savic D, Opačić M, Nestorov J, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Zorović M, Živin M, Selih VS, Spasic S, Spasojević I. The importance of copper in pathology of mesial temporal lobe epilepsy. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:46.
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

Savic, Danijela, Opačić, Miloš, Nestorov, Jelena, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Zorović, Maja, Živin, Marko, Selih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "The importance of copper in pathology of mesial temporal lobe epilepsy" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):46,
https://hdl.handle.net/21.15107/rcub_rimsi_2841 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Zorović, Maja
AU  - Savic, Danijela
AU  - Živin, Marko
AU  - Raičević, Savo
AU  - Bascarevic, Vladimir
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2852
AB  - Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.
PB  - FENS
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients
SP  - 296
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2852
ER  - 

@conference{
author = "Opačić, Miloš and Zorović, Maja and Savic, Danijela and Živin, Marko and Raičević, Savo and Bascarevic, Vladimir and Ristic, Aleksandar J and Sokic, Dragoslav and Spasojević, Ivan",
year = "2019",
abstract = "Aims: A drop in copper level and the loss of energy homeostasis are both portrayed in mesial temporal lobe epilepsy (mTLE) with hippocampal sclerosis (HS) patients. Cytochrome c oxidase (COX) represents a crossroad of energy and copper metabolism; it is a key component of mitochondrial machinery and contains two copper centers. Our aim here was to examine the link between COX activity and the copper transporting system in HS. COX activity and the levels of mRNA of selected chaperones - COX11, COX17, Sco1 and Sco2 were determined in 13 anatomically distinct hippocampal regions. Methods: Study was performed on seven hippocampal samples, four of which had been acquired during the course of amygdalohippocampectomy treatment of medically intractable epilepsy and three control postmortem samples. Adjacent slices were used for Nissl staining, COX activity assay and mRNA in situ hybridization with autoradiography. Densitometry was performed using ImageJ. Results: Overall COX activity was decreased in HS compared to controls (P = 0.0003). However, 5 regions showed significantly lower COX activity in HS and 8 did not. Subiculum showed slightly higher activity in HS. The levels of mRNA levels were lowered in HS in 6 regions for COX11, 10 regions for COX17, two regions for Sco1 and 11 regions for Sco2. Conclusions: Our findings suggest the loss of energy homeostasis in HS may be related to pathological changes in specific components of copper delivery to COX, and that the impact may vary between different hippocampal regions.",
publisher = "FENS",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients",
pages = "296",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2852"
}

Opačić, M., Zorović, M., Savic, D., Živin, M., Raičević, S., Bascarevic, V., Ristic, A. J., Sokic, D.,& Spasojević, I.. (2019). Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
FENS., 296.
https://hdl.handle.net/21.15107/rcub_rimsi_2852

Opačić M, Zorović M, Savic D, Živin M, Raičević S, Bascarevic V, Ristic AJ, Sokic D, Spasojević I. Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;:296.
https://hdl.handle.net/21.15107/rcub_rimsi_2852 .

Opačić, Miloš, Zorović, Maja, Savic, Danijela, Živin, Marko, Raičević, Savo, Bascarevic, Vladimir, Ristic, Aleksandar J, Sokic, Dragoslav, Spasojević, Ivan, "Relationship Between Regional Distributions of Cytochrome C Oxidase and Copper-Delivering Chaperones in Sclerotic Hippocampi of Epilepsy Patients" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia (2019):296,
https://hdl.handle.net/21.15107/rcub_rimsi_2852 .

TY  - CONF
AU  - Opačić, Miloš
AU  - Nestorov, Jelena
AU  - Tasić, Danica
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Bascarevic, Vladimir
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2810
AB  - Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.
PB  - Društvo biologa Srbije
C3  - Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
T1  - Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2810
ER  - 

@conference{
author = "Opačić, Miloš and Nestorov, Jelena and Tasić, Danica and Bogdanović Pristov, Jelena and Savić, Danijela Z and Bascarevic, Vladimir and Ristic, Aleksandar J and Sokic, Dragoslav and Spasojević, Ivan",
year = "2018",
abstract = "Skleroza hipokampusa je najčešći neuropatološki nalaz kod epilepsije temporalnog
režnja. Pored morfoloških promena odlikuju ga i promene nivoa različitih proteina u
ćeliji. Imunoblot je nezamenjiva metoda za određivanje nivoa proteina i podrazumeva
normalizaciju u odnosu na endogenu kontrolu (kontrolu jednakog nanošenja uzorka). Za
tu svrhu se koriste proteini za koje se smatra da imaju stabilnu ekspresiju koja najmanje
varira u ispitivanim uslovima, tretmanima i patofiziološkim stanjima. Ipak u nekim
patofiziološkim stanjima se i ekspresija tradicionalno korišćenih endogenih kontrola
menja,
1
što može dovesti do prikupljanja netačnih podataka i pogrešnog tumačenja
rezultata. Cilj ovog rada je bio da se ispita stabilnost najčešće korišćenih endogenih
kontrola i izabere najstabilnija koja će se koristiti za normalizaciju pri poređenju nivoa
ciljnih proteina hipokampusa obolelih od epilepsije i neurološki intaktnih kontrola.
Imunoblotom su detektovani β-aktin, α-tubulin, TATA-vezujući protein i gliceraldehid3-fosfat dehidrogenaza u tkivnim ekstraktima hipokampusa 9 pacijenata i 7 kontrola, i
NormFinder softverom2
određene unutargrupna i međugrupna varijabilnost. Pokazano je
da nivo strukturnih proteina β-aktina i α-tubulina najmanje varira u ispitivanim uslovima
te se mogu koristiti za poređenje nivoa proteina u sklerotičnim i intaktnim
hipokampusima. Rezultati upućuju da je odabir endogenih kontrola važan preduslov za
dobijanje tačnih i pouzdanih podataka imunoblot metodom.",
publisher = "Društvo biologa Srbije",
journal = "Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave",
title = "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2810"
}

Opačić, M., Nestorov, J., Tasić, D., Bogdanović Pristov, J., Savić, D. Z., Bascarevic, V., Ristic, A. J., Sokic, D.,& Spasojević, I.. (2018). Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave
Društvo biologa Srbije., 164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810

Opačić M, Nestorov J, Tasić D, Bogdanović Pristov J, Savić DZ, Bascarevic V, Ristic AJ, Sokic D, Spasojević I. Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja. in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave. 2018;:164.
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

Opačić, Miloš, Nestorov, Jelena, Tasić, Danica, Bogdanović Pristov, Jelena, Savić, Danijela Z, Bascarevic, Vladimir, Ristic, Aleksandar J, Sokic, Dragoslav, Spasojević, Ivan, "Odabir endogene kontrole za imunoblot analizu proteina u sklerotičnim hipokampusima pacijenata sa epilepsijom temporalnog režnja" in Drugi kongres biologa Srbije Osnovna i primenjena istraživanja metodika nastave (2018):164,
https://hdl.handle.net/21.15107/rcub_rimsi_2810 .

TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2961
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.
PB  - Biohemijsko društvo Srbije
C3  - Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 112
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2961
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.",
publisher = "Biohemijsko društvo Srbije",
journal = "Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "112-105",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2961"
}

Savic, D., Opačić, M., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasic, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
Biohemijsko društvo Srbije., 105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961

Savic D, Opačić M, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Živin M, Šelih VS, Spasic S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

Savic, Danijela, Opačić, Miloš, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica D
AU  - Baščarević, Vladimir
AU  - Zivić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1115
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Antioxidants & Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
EP  - 1575
IS  - 17
SP  - 1570
VL  - 28
DO  - 10.1089/ars.2017.7433
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica D and Baščarević, Vladimir and Zivić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Antioxidants & Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
pages = "1575-1570",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433"
}

Opačić, M., Stević, Z. D., Baščarević, V., Zivić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling
Mary Ann Liebert, Inc, New Rochelle., 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević ZD, Baščarević V, Zivić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica D, Baščarević, Vladimir, Zivić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants & Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - CHAP
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Savic, Danijela
AU  - Selih, Vid Simon
AU  - Zivin, Marko
AU  - Sokic, Dragoslav
AU  - Raičević, Savo
AU  - Bascarevic, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2938
AB  - Mesial temporal lobe epilepsy (mTLE) represents the most common subtype of human
focal epilepsies and perhaps the best-characterized disorder of this type 1
. Hippocampal
sclerosis (HS) is the most common histopathologic abnormality found in adults with drugresistant mTLE 2
. The histopathologic hallmark of HS is segmental pyramidal cell loss,
which can affect any field of the cornu Ammonis (CA1-4). Hippocampal neuronal cell loss
is always associated with a severe pattern of astrogliosis 3
. Therewithal, disturbed
homeostasis of metals is implicated in the pathology of mTLE-HS. Zinc has been
considered to play a major role in epileptogenesis in relation to its involvement in the
modulation of excitability and synaptic plasticity 4,5. Further, it has been shown that
epileptogenic hippocampi are exposed to oxidative stress and that the development of prooxidative conditions in the CNS usually involves the loss of homeostasis of iron 6,7. Low
brain levels of copper and manganese have been reported in patients with Menkes disease
and in animal models of epilepsy, and linked to seizure development 8,9. Ristić et al. were
the first to conduct a case-control study of total concentration of metals in tissue of human
HS, and report lower concentrations of copper and manganese 10.
PB  - Biohemijsko društvo Srbije
T2  - Biochemistry of Control in Life and Technology
T1  - Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 181
SP  - 179
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2938
ER  - 

@inbook{
author = "Opačić, Miloš and Ristic, Aleksandar J and Savic, Danijela and Selih, Vid Simon and Zivin, Marko and Sokic, Dragoslav and Raičević, Savo and Bascarevic, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "Mesial temporal lobe epilepsy (mTLE) represents the most common subtype of human
focal epilepsies and perhaps the best-characterized disorder of this type 1
. Hippocampal
sclerosis (HS) is the most common histopathologic abnormality found in adults with drugresistant mTLE 2
. The histopathologic hallmark of HS is segmental pyramidal cell loss,
which can affect any field of the cornu Ammonis (CA1-4). Hippocampal neuronal cell loss
is always associated with a severe pattern of astrogliosis 3
. Therewithal, disturbed
homeostasis of metals is implicated in the pathology of mTLE-HS. Zinc has been
considered to play a major role in epileptogenesis in relation to its involvement in the
modulation of excitability and synaptic plasticity 4,5. Further, it has been shown that
epileptogenic hippocampi are exposed to oxidative stress and that the development of prooxidative conditions in the CNS usually involves the loss of homeostasis of iron 6,7. Low
brain levels of copper and manganese have been reported in patients with Menkes disease
and in animal models of epilepsy, and linked to seizure development 8,9. Ristić et al. were
the first to conduct a case-control study of total concentration of metals in tissue of human
HS, and report lower concentrations of copper and manganese 10.",
publisher = "Biohemijsko društvo Srbije",
journal = "Biochemistry of Control in Life and Technology",
booktitle = "Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "181-179",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2938"
}

Opačić, M., Ristic, A. J., Savic, D., Selih, V. S., Zivin, M., Sokic, D., Raičević, S., Bascarevic, V.,& Spasojević, I.. (2017). Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Biochemistry of Control in Life and Technology
Biohemijsko društvo Srbije., 179-181.
https://hdl.handle.net/21.15107/rcub_rimsi_2938

Opačić M, Ristic AJ, Savic D, Selih VS, Zivin M, Sokic D, Raičević S, Bascarevic V, Spasojević I. Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Biochemistry of Control in Life and Technology. 2017;:179-181.
https://hdl.handle.net/21.15107/rcub_rimsi_2938 .

Opačić, Miloš, Ristic, Aleksandar J, Savic, Danijela, Selih, Vid Simon, Zivin, Marko, Sokic, Dragoslav, Raičević, Savo, Bascarevic, Vladimir, Spasojević, Ivan, "Imaging and regional distribution of copper, zinc, manganese and iron in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Biochemistry of Control in Life and Technology (2017):179-181,
https://hdl.handle.net/21.15107/rcub_rimsi_2938 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Selih, Vid Simon
AU  - Zivin, Marko
AU  - Sokic, Dragoslav
AU  - Raicević, Savo
AU  - Baščarević, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1080
AB  - The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 148
IS  - 2
SP  - 141
VL  - 9
DO  - 10.1039/c6mt00293e
ER  - 

@article{
author = "Opačić, Miloš and Ristic, Aleksandar J and Savić, Danijela Z and Selih, Vid Simon and Zivin, Marko and Sokic, Dragoslav and Raicević, Savo and Baščarević, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "148-141",
number = "2",
volume = "9",
doi = "10.1039/c6mt00293e"
}

Opačić, M., Ristic, A. J., Savić, D. Z., Selih, V. S., Zivin, M., Sokic, D., Raicević, S., Baščarević, V.,& Spasojević, I.. (2017). Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics
Royal Soc Chemistry, Cambridge., 9(2), 141-148.
https://doi.org/10.1039/c6mt00293e

Opačić M, Ristic AJ, Savić DZ, Selih VS, Zivin M, Sokic D, Raicević S, Baščarević V, Spasojević I. Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics. 2017;9(2):141-148.
doi:10.1039/c6mt00293e .

Opačić, Miloš, Ristic, Aleksandar J, Savić, Danijela Z, Selih, Vid Simon, Zivin, Marko, Sokic, Dragoslav, Raicević, Savo, Baščarević, Vladimir, Spasojević, Ivan, "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Metallomics, 9, no. 2 (2017):141-148,
https://doi.org/10.1039/c6mt00293e . .

TY  - CONF
AU  - Opačić, Miloš
AU  - Stevic, Zorica
AU  - Živić, Miroslav
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2957
AB  - Background: ALS is an oxidative stress-related fastpaced
motor neuron disease for which there are no
accurate biomarkers of progression. This largely hampers
the development of therapeutics. Our aim was to test the
applicability of oxidation-reduction potential (ORP) of
cerebrospinal fluid (CSF) in ALS progression follow-up.
Methods: ORP was measured using RedoxSYS (Aytu
BioScience, Inc.) in CSF of 49 ALS patients (mean age
63 1.29 years; range 43-80 years; mean ALSFRSr score
37.47 0.90, range 21-48; m/f = 35/14) and 15 controls
(mean age 48.93 3.68 years, range 21-67 years; m/f =
10/5).
Results: Pearson correlation coefficient (R) between
ALSFRSr score and ORP was 0.27 (p = 0.06). R was
higher ( 0.45) when two outlier values were excluded
from the calculus. Importantly, ALS patients had significantly
higher mean ORP (ALS: 121.83 2.76mV;
controls: 111.14 2.94 mV; p = 0.033). The difference
was also significant for ORP normalized to age. Of note,
ORP is reciprocally proportional to the pro-oxidative
settings in biological samples.
Discussion: Increased ORP values in ALS patients
further confirm the role of oxidative stress in this
neurodegenerative disease. ORP might find application
as a biomarker for ALS progression/prognosis but further
measurements on a larger cohort is warranted.
PB  - Taylor and Francis
C3  - Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
T1  - Oxidation-reduction potential of cerebrospinal fluid as a potential biomarker for ALS progression
SP  - 183
DO  - 10.1080/21678421.2016.1232061
ER  - 

@conference{
author = "Opačić, Miloš and Stevic, Zorica and Živić, Miroslav and Spasojević, Ivan",
year = "2016",
abstract = "Background: ALS is an oxidative stress-related fastpaced
motor neuron disease for which there are no
accurate biomarkers of progression. This largely hampers
the development of therapeutics. Our aim was to test the
applicability of oxidation-reduction potential (ORP) of
cerebrospinal fluid (CSF) in ALS progression follow-up.
Methods: ORP was measured using RedoxSYS (Aytu
BioScience, Inc.) in CSF of 49 ALS patients (mean age
63 1.29 years; range 43-80 years; mean ALSFRSr score
37.47 0.90, range 21-48; m/f = 35/14) and 15 controls
(mean age 48.93 3.68 years, range 21-67 years; m/f =
10/5).
Results: Pearson correlation coefficient (R) between
ALSFRSr score and ORP was 0.27 (p = 0.06). R was
higher ( 0.45) when two outlier values were excluded
from the calculus. Importantly, ALS patients had significantly
higher mean ORP (ALS: 121.83 2.76mV;
controls: 111.14 2.94 mV; p = 0.033). The difference
was also significant for ORP normalized to age. Of note,
ORP is reciprocally proportional to the pro-oxidative
settings in biological samples.
Discussion: Increased ORP values in ALS patients
further confirm the role of oxidative stress in this
neurodegenerative disease. ORP might find application
as a biomarker for ALS progression/prognosis but further
measurements on a larger cohort is warranted.",
publisher = "Taylor and Francis",
journal = "Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration",
title = "Oxidation-reduction potential of cerebrospinal fluid as a potential biomarker for ALS progression",
pages = "183",
doi = "10.1080/21678421.2016.1232061"
}

Opačić, M., Stevic, Z., Živić, M.,& Spasojević, I.. (2016). Oxidation-reduction potential of cerebrospinal fluid as a potential biomarker for ALS progression. in Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration
Taylor and Francis., 183.
https://doi.org/10.1080/21678421.2016.1232061

Opačić M, Stevic Z, Živić M, Spasojević I. Oxidation-reduction potential of cerebrospinal fluid as a potential biomarker for ALS progression. in Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration. 2016;:183.
doi:10.1080/21678421.2016.1232061 .

Opačić, Miloš, Stevic, Zorica, Živić, Miroslav, Spasojević, Ivan, "Oxidation-reduction potential of cerebrospinal fluid as a potential biomarker for ALS progression" in Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration (2016):183,
https://doi.org/10.1080/21678421.2016.1232061 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Babic, Nikolina
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/921
AB  - We have developed a simple one-step 30-min method for fluorescent visualization of proteins in native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) gels. The method is based on formation of strong fluorophores via potassium ferricyanide-provoked oxidation of tryptophan (Trp). Following PAGE, gels are soaked in water solution of potassium ferricyanide (100 mM) and NaOH (1 M) and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method was slightly lower compared with standard Coomassie Brilliant Blue (CBB) staining. The method can be useful when rapid acquisition of data is of the essence. After preview, gels can be post-stained using the CBB protocol for further analysis. The intensity of fluorescence is dependent on Trp number, so the protocol might find application in the quantification of Trp residues as illustrated here. Importantly, there is room for improvement of the method. Namely, according to excitation-emission matrix analysis of stained protein bands, maximal fluorescence intensity (at 345/460 nm) was 3.5-fold higher compared with the settings that were available on a commercial imager (395/525 nm). As a supplement, we present an upgrade of the previously described method for in-gel detection of non-heme iron-binding proteins that also employs potassium ferricyanide.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Analytical Biochemistry
T1  - A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis
EP  - 10
SP  - 6
VL  - 480
DO  - 10.1016/j.ab.2015.04.006
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Opačić, Miloš and Dimitrijević, Milena and Babic, Nikolina and Spasojević, Ivan",
year = "2015",
abstract = "We have developed a simple one-step 30-min method for fluorescent visualization of proteins in native and sodium dodecyl sulfate polyacrylamide gel electrophoresis (PAGE) gels. The method is based on formation of strong fluorophores via potassium ferricyanide-provoked oxidation of tryptophan (Trp). Following PAGE, gels are soaked in water solution of potassium ferricyanide (100 mM) and NaOH (1 M) and are kept in the dark for 30 min. Gels are then transferred to water and scanned. The sensitivity of the method was slightly lower compared with standard Coomassie Brilliant Blue (CBB) staining. The method can be useful when rapid acquisition of data is of the essence. After preview, gels can be post-stained using the CBB protocol for further analysis. The intensity of fluorescence is dependent on Trp number, so the protocol might find application in the quantification of Trp residues as illustrated here. Importantly, there is room for improvement of the method. Namely, according to excitation-emission matrix analysis of stained protein bands, maximal fluorescence intensity (at 345/460 nm) was 3.5-fold higher compared with the settings that were available on a commercial imager (395/525 nm). As a supplement, we present an upgrade of the previously described method for in-gel detection of non-heme iron-binding proteins that also employs potassium ferricyanide.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Analytical Biochemistry",
title = "A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis",
pages = "10-6",
volume = "480",
doi = "10.1016/j.ab.2015.04.006"
}

Bogdanović Pristov, J., Opačić, M., Dimitrijević, M., Babic, N.,& Spasojević, I.. (2015). A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. in Analytical Biochemistry
Academic Press Inc Elsevier Science, San Diego., 480, 6-10.
https://doi.org/10.1016/j.ab.2015.04.006

Bogdanović Pristov J, Opačić M, Dimitrijević M, Babic N, Spasojević I. A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis. in Analytical Biochemistry. 2015;480:6-10.
doi:10.1016/j.ab.2015.04.006 .

Bogdanović Pristov, Jelena, Opačić, Miloš, Dimitrijević, Milena, Babic, Nikolina, Spasojević, Ivan, "A method for in-gel fluorescent visualization of proteins after native and sodium dodecyl sulfate polyacrylamide gel electrophoresis" in Analytical Biochemistry, 480 (2015):6-10,
https://doi.org/10.1016/j.ab.2015.04.006 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Maglic, Dragana
AU  - Opačić, Miloš
AU  - Mandic, Vesna
AU  - Miković, Zeljko
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/585
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Thrombosis Research
T1  - Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin
EP  - 829
IS  - 5
SP  - 826
VL  - 130
DO  - 10.1016/j.thromres.2012.08.310
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Maglic, Dragana and Opačić, Miloš and Mandic, Vesna and Miković, Zeljko and Spasić, Mihajlo and Spasojević, Ivan",
year = "2012",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Thrombosis Research",
title = "Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin",
pages = "829-826",
number = "5",
volume = "130",
doi = "10.1016/j.thromres.2012.08.310"
}

Bogdanović Pristov, J., Maglic, D., Opačić, M., Mandic, V., Miković, Z., Spasić, M.,& Spasojević, I.. (2012). Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin. in Thrombosis Research
Pergamon-Elsevier Science Ltd, Oxford., 130(5), 826-829.
https://doi.org/10.1016/j.thromres.2012.08.310

Bogdanović Pristov J, Maglic D, Opačić M, Mandic V, Miković Z, Spasić M, Spasojević I. Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin. in Thrombosis Research. 2012;130(5):826-829.
doi:10.1016/j.thromres.2012.08.310 .

Bogdanović Pristov, Jelena, Maglic, Dragana, Opačić, Miloš, Mandic, Vesna, Miković, Zeljko, Spasić, Mihajlo, Spasojević, Ivan, "Ante- and postpartum redox status of blood in women with inherited thrombophilia treated with heparin" in Thrombosis Research, 130, no. 5 (2012):826-829,
https://doi.org/10.1016/j.thromres.2012.08.310 . .