Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess
Аутори
Vojvodić, SnežanaOpačić, Miloš
Stanić, Marina
Dimitrijević, Milena
Pittman, Jon K.
Spasojević, Ivan
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand..., the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.
Кључне речи:
microalgae / nickel / ROS / photosystem IIИзвор:
Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia, 2022, 133-133Издавач:
- Faculty of Chemistry, Serbian Biochemical Society
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- NATO Science for Peace and Security Programme - G5320
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Vojvodić, Snežana AU - Opačić, Miloš AU - Stanić, Marina AU - Dimitrijević, Milena AU - Pittman, Jon K. AU - Spasojević, Ivan PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2417 AB - Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery. PB - Faculty of Chemistry, Serbian Biochemical Society C3 - Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia T1 - Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess EP - 133 SP - 133 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2417 ER -
@conference{ author = "Vojvodić, Snežana and Opačić, Miloš and Stanić, Marina and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan", year = "2022", abstract = "Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.", publisher = "Faculty of Chemistry, Serbian Biochemical Society", journal = "Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia", title = "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess", pages = "133-133", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2417" }
Vojvodić, S., Opačić, M., Stanić, M., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2022). Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia Faculty of Chemistry, Serbian Biochemical Society., 133-133. https://hdl.handle.net/21.15107/rcub_rimsi_2417
Vojvodić S, Opačić M, Stanić M, Dimitrijević M, Pittman JK, Spasojević I. Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia. 2022;:133-133. https://hdl.handle.net/21.15107/rcub_rimsi_2417 .
Vojvodić, Snežana, Opačić, Miloš, Stanić, Marina, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess" in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia (2022):133-133, https://hdl.handle.net/21.15107/rcub_rimsi_2417 .