TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2216
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.
PB  - Springer Nature
T2  - Springer Nature, Ecotoxicology 32, 583–597 (2023)
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
EP  - 597
IS  - 32
SP  - 583
DO  - https://doi.org/10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.",
publisher = "Springer Nature",
journal = "Springer Nature, Ecotoxicology 32, 583–597 (2023)",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
pages = "597-583",
number = "32",
doi = "https://doi.org/10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023)
Springer Nature.(32), 583-597.
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023). 2023;(32):583-597.
doi:https://doi.org/10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Springer Nature, Ecotoxicology 32, 583–597 (2023), no. 32 (2023):583-597,
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Đorđević Aleksić, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2173
AB  - One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.
PB  - Serbian Society of Toxicology
C3  - 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2173
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić Jeftić, Željka and Subotić, Srđan and Đorđević Aleksić, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2023",
abstract = "One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.",
publisher = "Serbian Society of Toxicology",
journal = "13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2173"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić Jeftić, Ž., Subotić, S., Đorđević Aleksić, J., Lenhardt, M.,& Vuković-Gačić, B.. (2023). Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173

Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić Jeftić Ž, Subotić S, Đorđević Aleksić J, Lenhardt M, Vuković-Gačić B. Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić Jeftić, Željka, Subotić, Srđan, Đorđević Aleksić, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment." in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):72,
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1729
AB  - The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1729
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1729"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):69,
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1725
AB  - Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1725
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1725"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2166
AB  - У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.
PB  - Народна библиотека Србије
C3  - Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
T1  - Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2166
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.",
publisher = "Народна библиотека Србије",
journal = "Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia",
title = "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2166"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
Народна библиотека Србије., 155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka" in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kostić, Jovana
AU  - Kolarević, Stoimir
AU  - Jovanović Marić, Jovana
AU  - Nikolić, Dušan
AU  - Skorić, Stefan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3100
AB  - The objective of our study was to investigate ecological status of two water bodies in Serbia, Lake Kruščica, and River Ibar. The comet assay was selected as an in vivo and in situ test for measuring DNA damage in chub’s erythrocytes, as an bioindicator of genotoxic potential of water. We have also investigated physico-chemical parameters and microbiological indicators (coliforms, E.coli) as parameters for general pollution pressure and a basis for determination of ecological status of these water bodies. Lake Kruščica is a protected natural area of great importance with very low or none anthropogenic impact, used as a drinking water source. River Ibar is under intensive multisource pressure pollution consisted of: communal untreated waters, industrial, agricultural and mining activities. Specimens of chub analyzed in this study naturally inhabit these water bodies, which gave us an excellent opportunity to compare protected site with site where mixture of various xenobiotics from water have a direct and adverse impacts over period of time. According to physico-chemical parameters and microbiological indicators, Lake Kruščica falls into the first category of ecological status, while River Ibar showed poor to critical water quality. DNA damage in chub erythrocytes was significantly higher at River Ibar compared to Lake Kruscica, which indicates the notable difference in pollution pressure. Given its tolerance to anthropogenic perturbations, and its cosmopolite distributions, chub gives an advantage for extensive studies of genotoxicity dynamics of freshwater systems.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Genotoxicity of European chub (Squalius cephalus L. 1758) erythrocytes as an effective indicator in monitoring of water bodies under different pollution pressure
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3100
ER  - 

@conference{
author = "Sunjog, Karolina and Kostić, Jovana and Kolarević, Stoimir and Jovanović Marić, Jovana and Nikolić, Dušan and Skorić, Stefan",
year = "2022",
abstract = "The objective of our study was to investigate ecological status of two water bodies in Serbia, Lake Kruščica, and River Ibar. The comet assay was selected as an in vivo and in situ test for measuring DNA damage in chub’s erythrocytes, as an bioindicator of genotoxic potential of water. We have also investigated physico-chemical parameters and microbiological indicators (coliforms, E.coli) as parameters for general pollution pressure and a basis for determination of ecological status of these water bodies. Lake Kruščica is a protected natural area of great importance with very low or none anthropogenic impact, used as a drinking water source. River Ibar is under intensive multisource pressure pollution consisted of: communal untreated waters, industrial, agricultural and mining activities. Specimens of chub analyzed in this study naturally inhabit these water bodies, which gave us an excellent opportunity to compare protected site with site where mixture of various xenobiotics from water have a direct and adverse impacts over period of time. According to physico-chemical parameters and microbiological indicators, Lake Kruščica falls into the first category of ecological status, while River Ibar showed poor to critical water quality. DNA damage in chub erythrocytes was significantly higher at River Ibar compared to Lake Kruscica, which indicates the notable difference in pollution pressure. Given its tolerance to anthropogenic perturbations, and its cosmopolite distributions, chub gives an advantage for extensive studies of genotoxicity dynamics of freshwater systems.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Genotoxicity of European chub (Squalius cephalus L. 1758) erythrocytes as an effective indicator in monitoring of water bodies under different pollution pressure",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3100"
}

Sunjog, K., Kostić, J., Kolarević, S., Jovanović Marić, J., Nikolić, D.,& Skorić, S.. (2022). Genotoxicity of European chub (Squalius cephalus L. 1758) erythrocytes as an effective indicator in monitoring of water bodies under different pollution pressure. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY..
https://hdl.handle.net/21.15107/rcub_rimsi_3100

Sunjog K, Kostić J, Kolarević S, Jovanović Marić J, Nikolić D, Skorić S. Genotoxicity of European chub (Squalius cephalus L. 1758) erythrocytes as an effective indicator in monitoring of water bodies under different pollution pressure. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_3100 .

Sunjog, Karolina, Kostić, Jovana, Kolarević, Stoimir, Jovanović Marić, Jovana, Nikolić, Dušan, Skorić, Stefan, "Genotoxicity of European chub (Squalius cephalus L. 1758) erythrocytes as an effective indicator in monitoring of water bodies under different pollution pressure" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_3100 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2164
AB  - Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
PB  - Prirodno-matematički fakultet, UNS
C3  - 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
T1  - Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2164
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana",
year = "2022",
abstract = "Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.",
publisher = "Prirodno-matematički fakultet, UNS",
journal = "9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.",
title = "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2164"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K.,& Kostić-Vuković, J.. (2022). Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
Prirodno-matematički fakultet, UNS., 2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J. Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.. 2022;2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava" in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia., 2 (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Smederevac-Lalić, Marija
AU  - Marković, Zoran
AU  - Skorić, Stefan
AU  - Jaćimović, Milica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1694
AB  - Invasive alien fish species represent one of the greatest threats to the biodiversity and ecological status of freshwater ecosystems. Within their management, their application as bioindicators of pollution in monitoring programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay. Sampling was performed from June to September 2021 on Markovačko Lake, which is exposed to diffuse pollution from the application of pesticides on the surrounding agricultural land. Monitoring also included the assessment of water quality by basic physical and chemical parameters, as well as microbiological indicators of faecal pollution. Alkaline comet assay was performed on fish erythrocytes, on ten specimens per month. The study also included ten control fish from the recirculating aquaculture system (RAS), which were transferred from Markovačko Lake for breeding, and treatment of their erythrocytes with H2O2 as a positive control. Scoring of comets was performed by CometScore 2.0 software. The lowest level of DNA damage was recorded in the control group and the highest in the positive control group. August was the month with the lowest level of DNA damage, and September with the highest. Statistically significant differences were found between all groups indicating a high sensitivity of the alkaline comet assay to discriminate fine differences in the genotoxic potential of water. Knowing that black bullhead is highly abundant in stagnant waters and relatively easy to sample, this study draws additional attention to its use as a bioindicator of genotoxicity.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1694
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Smederevac-Lalić, Marija and Marković, Zoran and Skorić, Stefan and Jaćimović, Milica",
year = "2022",
abstract = "Invasive alien fish species represent one of the greatest threats to the biodiversity and ecological status of freshwater ecosystems. Within their management, their application as bioindicators of pollution in monitoring programs should be considered. The aim of this study was to examine the
use of black bullhead Ameiurus melas (Rafinesque, 1820), invasive alien fish in Serbia, as a bioindicator of water genotoxicity by alkaline comet assay. Sampling was performed from June to September 2021 on Markovačko Lake, which is exposed to diffuse pollution from the application of pesticides on the surrounding agricultural land. Monitoring also included the assessment of water quality by basic physical and chemical parameters, as well as microbiological indicators of faecal pollution. Alkaline comet assay was performed on fish erythrocytes, on ten specimens per month. The study also included ten control fish from the recirculating aquaculture system (RAS), which were transferred from Markovačko Lake for breeding, and treatment of their erythrocytes with H2O2 as a positive control. Scoring of comets was performed by CometScore 2.0 software. The lowest level of DNA damage was recorded in the control group and the highest in the positive control group. August was the month with the lowest level of DNA damage, and September with the highest. Statistically significant differences were found between all groups indicating a high sensitivity of the alkaline comet assay to discriminate fine differences in the genotoxic potential of water. Knowing that black bullhead is highly abundant in stagnant waters and relatively easy to sample, this study draws additional attention to its use as a bioindicator of genotoxicity.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1694"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Smederevac-Lalić, M., Marković, Z., Skorić, S.,& Jaćimović, M.. (2022). Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY..
https://hdl.handle.net/21.15107/rcub_rimsi_1694

Kostić-Vuković J, Sunjog K, Kolarević S, Smederevac-Lalić M, Marković Z, Skorić S, Jaćimović M. Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;.
https://hdl.handle.net/21.15107/rcub_rimsi_1694 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Smederevac-Lalić, Marija, Marković, Zoran, Skorić, Stefan, Jaćimović, Milica, "Sensitivity of invasive alien fish in Serbia black bullhead Ameiurus melas (Rafinesque, 1820) as a bioindicator of genotoxicity" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_1694 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1752
AB  - Непречишћене комуналне и индустријске отпадне воде представљају извор 
хемијског и микробиолошког загађења водених екосистема и угрожавају све 
видове њиховог коришћења. Ово истраживање је спроведено у јулу 2017. године, 
на реци Дунав, локалитету Нови Бановци, који је изложен испусту колектора 
непречишћених индустријских и комуналних отпадних вода насеља са око 40 000 
становника. Вода је узоркована за микробиолошку анализу индикатора фекалног 
загађења ензимском методом Colilert (IDEXX). Неколико десетина метара од 
испуста колектора узорковано је 5 јединки шљивара Vimba vimba (L., 1758) за 
анализу метала у мишићу и јетри методом ICP-OES и нивоа ДНК оштећења у 
ћелијама крви, јетре и шкрга применом алкалног комет теста. Према нашим 
сазнањима ово је прва студија у којој је комет тест примењен на ћелијама 
шљивара. На основу индикатора фекалног загађења (укупни колиформи и E. coli) 
вода се могла класификовати као критично загађена (III класа). Највиши ниво ДНК 
оштећења забележен је у ћелијама крви, потом следе шкрге и јетра. Ниво ДНК 
оштећења у ћелијама крви био је статистички значајно виши у односу на јетру. На 
основу концентрација Fe и Zn, за које су прописане максимално дозвољене 
концентрације за рибље производе у лименој амбалажи, месо шљивара је безбедно 
за исхрану људи.
PB  - Српско биолошко друштво
C3  - ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
T1  - Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.
SP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1752
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2022",
abstract = "Непречишћене комуналне и индустријске отпадне воде представљају извор 
хемијског и микробиолошког загађења водених екосистема и угрожавају све 
видове њиховог коришћења. Ово истраживање је спроведено у јулу 2017. године, 
на реци Дунав, локалитету Нови Бановци, који је изложен испусту колектора 
непречишћених индустријских и комуналних отпадних вода насеља са око 40 000 
становника. Вода је узоркована за микробиолошку анализу индикатора фекалног 
загађења ензимском методом Colilert (IDEXX). Неколико десетина метара од 
испуста колектора узорковано је 5 јединки шљивара Vimba vimba (L., 1758) за 
анализу метала у мишићу и јетри методом ICP-OES и нивоа ДНК оштећења у 
ћелијама крви, јетре и шкрга применом алкалног комет теста. Према нашим 
сазнањима ово је прва студија у којој је комет тест примењен на ћелијама 
шљивара. На основу индикатора фекалног загађења (укупни колиформи и E. coli) 
вода се могла класификовати као критично загађена (III класа). Највиши ниво ДНК 
оштећења забележен је у ћелијама крви, потом следе шкрге и јетра. Ниво ДНК 
оштећења у ћелијама крви био је статистички значајно виши у односу на јетру. На 
основу концентрација Fe и Zn, за које су прописане максимално дозвољене 
концентрације за рибље производе у лименој амбалажи, месо шљивара је безбедно 
за исхрану људи.",
publisher = "Српско биолошко друштво",
journal = "ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ",
title = "Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1752"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Vuković-Gačić, B.,& Lenhardt, M.. (2022). Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
Српско биолошко друштво., 156.
https://hdl.handle.net/21.15107/rcub_rimsi_1752

Kostić-Vuković J, Kolarević S, Sunjog K, Višnjić Jeftić Ž, Subotić S, Vuković-Gačić B, Lenhardt M. Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ. 2022;:156.
https://hdl.handle.net/21.15107/rcub_rimsi_1752 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija." in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ (2022):156,
https://hdl.handle.net/21.15107/rcub_rimsi_1752 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović Milutinović, Danijela
AU  - Kirschner, Alexander K. T.
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Djukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1920
AB  - Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1920
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Jovanović Marić, Jovana and Vojnović Milutinović, Danijela and Kirschner, Alexander K. T. and Farnleitner, Andreas and Linke, Rita and Djukic, Aleksandar and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1920"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Jovanović Marić, J., Vojnović Milutinović, D., Kirschner, A. K. T., Farnleitner, A., Linke, R., Djukic, A., Kostić-Vuković, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)., 614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Jovanović Marić J, Vojnović Milutinović D, Kirschner AKT, Farnleitner A, Linke R, Djukic A, Kostić-Vuković J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Jovanović Marić, Jovana, Vojnović Milutinović, Danijela, Kirschner, Alexander K. T., Farnleitner, Andreas, Linke, Rita, Djukic, Aleksandar, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):614,
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Vojnović Milutinović, Danijela
AU  - Jovanović-Marić, Jovana
AU  - K.T. Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1566
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters
SP  - 156964
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Vojnović Milutinović, Danijela and Jovanović-Marić, Jovana and K.T. Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukic, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters",
pages = "156964",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Vojnović Milutinović, D., Jovanović-Marić, J., K.T. Kirschner, A., Farnleitner, A., Linke, R., Đukic, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment
Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Vojnović Milutinović D, Jovanović-Marić J, K.T. Kirschner A, Farnleitner A, Linke R, Đukic A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Vojnović Milutinović, Danijela, Jovanović-Marić, Jovana, K.T. Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukic, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters" in Science of the Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1688
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.
PB  - Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany
C3  - 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1688
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.",
publisher = "Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany",
journal = "43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1688"
}

Đorđević Aleksić, J., Kostić-Vuković, J., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany..
https://hdl.handle.net/21.15107/rcub_rimsi_1688

Đorđević Aleksić J, Kostić-Vuković J, Sunjog K, Višnjić Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;.
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

Đorđević Aleksić, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods." in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1501
AB  - This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 193(8).
https://doi.org/10.1007/s10661-021-09232-6

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Gačić Z, Lenhardt M, Vukovic-Gacic B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8).
doi:10.1007/s10661-021-09232-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021),
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Kolarević, Stoimir
AU  - Milutinovic-Smiljanic, Sanja
AU  - Mandic, Jelena
AU  - Vukovic-Gacic, Branka
AU  - Bulat, Zorica
AU  - Curcic, Marijana
AU  - Kracun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Jovanović-Marić, Jovana
AU  - Antonijevic-Miljaković, Evica
AU  - Dukic-Cosic, Danijela
AU  - Đorđević, Aleksandra Buha
AU  - Javorac, Dragana
AU  - Baralic, Katarina
AU  - Mandinic, Zoran
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1505
AB  - The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140-190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Genotoxicity of fluoride subacute exposure in rats and selenium intervention
VL  - 266
DO  - 10.1016/j.chemosphere.2020.128978
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Kolarević, Stoimir and Milutinovic-Smiljanic, Sanja and Mandic, Jelena and Vukovic-Gacic, Branka and Bulat, Zorica and Curcic, Marijana and Kracun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Jovanović-Marić, Jovana and Antonijevic-Miljaković, Evica and Dukic-Cosic, Danijela and Đorđević, Aleksandra Buha and Javorac, Dragana and Baralic, Katarina and Mandinic, Zoran",
year = "2021",
abstract = "The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140-190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Genotoxicity of fluoride subacute exposure in rats and selenium intervention",
volume = "266",
doi = "10.1016/j.chemosphere.2020.128978"
}

Radovanović, J., Antonijević, B., Kolarević, S., Milutinovic-Smiljanic, S., Mandic, J., Vukovic-Gacic, B., Bulat, Z., Curcic, M., Kracun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Jovanović-Marić, J., Antonijevic-Miljaković, E., Dukic-Cosic, D., Đorđević, A. B., Javorac, D., Baralic, K.,& Mandinic, Z.. (2021). Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 266.
https://doi.org/10.1016/j.chemosphere.2020.128978

Radovanović J, Antonijević B, Kolarević S, Milutinovic-Smiljanic S, Mandic J, Vukovic-Gacic B, Bulat Z, Curcic M, Kracun-Kolarević M, Sunjog K, Kostić-Vuković J, Jovanović-Marić J, Antonijevic-Miljaković E, Dukic-Cosic D, Đorđević AB, Javorac D, Baralic K, Mandinic Z. Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere. 2021;266.
doi:10.1016/j.chemosphere.2020.128978 .

Radovanović, Jelena, Antonijević, Biljana, Kolarević, Stoimir, Milutinovic-Smiljanic, Sanja, Mandic, Jelena, Vukovic-Gacic, Branka, Bulat, Zorica, Curcic, Marijana, Kracun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Jovanović-Marić, Jovana, Antonijevic-Miljaković, Evica, Dukic-Cosic, Danijela, Đorđević, Aleksandra Buha, Javorac, Dragana, Baralic, Katarina, Mandinic, Zoran, "Genotoxicity of fluoride subacute exposure in rats and selenium intervention" in Chemosphere, 266 (2021),
https://doi.org/10.1016/j.chemosphere.2020.128978 . .

TY  - JOUR
AU  - Jovanović-Maric, Jovana M.
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Smiljanic, Petar B.
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1369
AB  - This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers
EP  - 229
IS  - 2
SP  - 224
VL  - 105
DO  - 10.1007/s00128-020-02944-4
ER  - 

@article{
author = "Jovanović-Maric, Jovana M. and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Smiljanic, Petar B. and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2020",
abstract = "This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers",
pages = "229-224",
number = "2",
volume = "105",
doi = "10.1007/s00128-020-02944-4"
}

Jovanović-Maric, J. M., Kracun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Smiljanic, P. B., Gačić, Z.,& Vukovic-Gacic, B.. (2020). Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 105(2), 224-229.
https://doi.org/10.1007/s00128-020-02944-4

Jovanović-Maric JM, Kracun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Smiljanic PB, Gačić Z, Vukovic-Gacic B. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology. 2020;105(2):224-229.
doi:10.1007/s00128-020-02944-4 .

Jovanović-Maric, Jovana M., Kracun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Smiljanic, Petar B., Gačić, Zoran, Vukovic-Gacic, Branka, "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers" in Bulletin of Environmental Contamination and Toxicology, 105, no. 2 (2020):224-229,
https://doi.org/10.1007/s00128-020-02944-4 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Kostić-Vuković, Jovana
AU  - Novaković, Irena
AU  - Jeremic, Marko
AU  - Sladic, Dusan
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1365
AB  - Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuCassay inSalmonella typhimuriumTA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models
EP  - 530
IS  - 5
SP  - 522
VL  - 43
DO  - 10.1080/01480545.2018.1514043
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović, Jovana and Kostić-Vuković, Jovana and Novaković, Irena and Jeremic, Marko and Sladic, Dusan and Vukovic-Gacic, Branka",
year = "2020",
abstract = "Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuCassay inSalmonella typhimuriumTA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models",
pages = "530-522",
number = "5",
volume = "43",
doi = "10.1080/01480545.2018.1514043"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, I., Jeremic, M., Sladic, D.,& Vukovic-Gacic, B.. (2020). Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor & Francis Ltd, Abingdon., 43(5), 522-530.
https://doi.org/10.1080/01480545.2018.1514043

Đorđević Aleksić J, Kolarević S, Jovanović J, Kostić-Vuković J, Novaković I, Jeremic M, Sladic D, Vukovic-Gacic B. Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2020;43(5):522-530.
doi:10.1080/01480545.2018.1514043 .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović, Jovana, Kostić-Vuković, Jovana, Novaković, Irena, Jeremic, Marko, Sladic, Dusan, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology, 43, no. 5 (2020):522-530,
https://doi.org/10.1080/01480545.2018.1514043 . .

TY  - CHAP
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, J.
AU  - Đorđević Aleksić, Jelena
AU  - Ilić, M.
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Divac, Rankov, A.
AU  - Ilić, B.
AU  - Pešić, V.
AU  - Vukovic-Gacic, Branka
AU  - Paunović, Momir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1302
AB  - Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Impact of pollution on rivers in Montenegro: Ecotoxicological perspective
EP  - 133
SP  - 111
VL  - 93
DO  - 10.1007/698_2019_425
ER  - 

@inbook{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, J. and Đorđević Aleksić, Jelena and Ilić, M. and Sunjog, Karolina and Kostić-Vuković, Jovana and Divac, Rankov, A. and Ilić, B. and Pešić, V. and Vukovic-Gacic, Branka and Paunović, Momir",
year = "2020",
abstract = "Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective",
pages = "133-111",
volume = "93",
doi = "10.1007/698_2019_425"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević Aleksić, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac, R. A., Ilić, B., Pešić, V., Vukovic-Gacic, B.,& Paunović, M.. (2020). Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 111-133.
https://doi.org/10.1007/698_2019_425

Kracun-Kolarević M, Kolarević S, Jovanović J, Đorđević Aleksić J, Ilić M, Sunjog K, Kostić-Vuković J, Divac RA, Ilić B, Pešić V, Vukovic-Gacic B, Paunović M. Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry. 2020;93:111-133.
doi:10.1007/698_2019_425 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, J., Đorđević Aleksić, Jelena, Ilić, M., Sunjog, Karolina, Kostić-Vuković, Jovana, Divac, Rankov, A., Ilić, B., Pešić, V., Vukovic-Gacic, Branka, Paunović, Momir, "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective" in Handbook of Environmental Chemistry, 93 (2020):111-133,
https://doi.org/10.1007/698_2019_425 . .

TY  - JOUR
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Deutschmann, Bjorn
AU  - Hollert, Henner
AU  - Tenji, Dina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1362
AB  - In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments
EP  - 33915
IS  - 27
SP  - 33903
VL  - 27
DO  - 10.1007/s11356-020-09597-0
ER  - 

@article{
author = "Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Sunjog, Karolina and Kostić-Vuković, Jovana and Deutschmann, Bjorn and Hollert, Henner and Tenji, Dina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2020",
abstract = "In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments",
pages = "33915-33903",
number = "27",
volume = "27",
doi = "10.1007/s11356-020-09597-0"
}

Jovanović-Marić, J., Kracun-Kolarević, M., Kolarević, S., Sunjog, K., Kostić-Vuković, J., Deutschmann, B., Hollert, H., Tenji, D., Paunović, M.,& Vukovic-Gacic, B.. (2020). Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(27), 33903-33915.
https://doi.org/10.1007/s11356-020-09597-0

Jovanović-Marić J, Kracun-Kolarević M, Kolarević S, Sunjog K, Kostić-Vuković J, Deutschmann B, Hollert H, Tenji D, Paunović M, Vukovic-Gacic B. Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research. 2020;27(27):33903-33915.
doi:10.1007/s11356-020-09597-0 .

Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Kolarević, Stoimir, Sunjog, Karolina, Kostić-Vuković, Jovana, Deutschmann, Bjorn, Hollert, Henner, Tenji, Dina, Paunović, Momir, Vukovic-Gacic, Branka, "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments" in Environmental Science and Pollution Research, 27, no. 27 (2020):33903-33915,
https://doi.org/10.1007/s11356-020-09597-0 . .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Jovanović, J.
AU  - Ilić, M.
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, S.
AU  - Joksimović, D.
AU  - Pešić, V.
AU  - Kirschner, A.K.T.
AU  - Linke, R.
AU  - Ixenmaier, S.
AU  - Farnleitner, Andreas
AU  - Savio, D.
AU  - Reischer, Georg
AU  - Tomić, N.
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1310
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Microbiological water quality of rivers in Montenegro
EP  - 155
SP  - 135
VL  - 93
DO  - 10.1007/698_2019_420
ER  - 

@inbook{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Jovanović, J. and Ilić, M. and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, S. and Joksimović, D. and Pešić, V. and Kirschner, A.K.T. and Linke, R. and Ixenmaier, S. and Farnleitner, Andreas and Savio, D. and Reischer, Georg and Tomić, N. and Vukovic-Gacic, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbiological water quality of rivers in Montenegro",
pages = "155-135",
volume = "93",
doi = "10.1007/698_2019_420"
}

Kolarević, S., Kracun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vukovic-Gacic, B.. (2020). Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 135-155.
https://doi.org/10.1007/698_2019_420

Kolarević S, Kracun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner A, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vukovic-Gacic B. Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry. 2020;93:135-155.
doi:10.1007/698_2019_420 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Jovanović, J., Ilić, M., Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, Andreas, Savio, D., Reischer, Georg, Tomić, N., Vukovic-Gacic, Branka, "Microbiological water quality of rivers in Montenegro" in Handbook of Environmental Chemistry, 93 (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kostić, Jovana
AU  - Lakota, Ajla
AU  - Aksović, Nevena
AU  - Kolarević, Stoimir
AU  - Gacic, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2462
AB  - This work represents the study of quality of the Danube River, in Belgrade, at the Višnjica
site, by analyzing the genotoxicity of water by using fish as bioindicators. With a population
of about 2 million inhabitants (Belgrade) and poor legislation regarding the discharge of
wastewaters, monitoring is the first step in approaching this serious problem. To obtain
information on the genotoxicity of water as a measure of DNA damage, the comet assay,
which detects the effects of exposure, and micronucleus test, which detects permanent
effect, were used. For the comet assay we have used three types of cells: blood, liver, and
gills, while the micronucleus test was done on blood cells. The fish used as bioindicators are
the common perch (Perca fluviatilis), vimba bream (Vimba vimba), common barbell (Barbus
barbus), and white bream (Blicca bjoerkna).
Regarding the comet assay, significant difference in DNA damage was observed
between species in all tested tissues. Vimba bream and common barbell had the
highest level of DNA damage in gills, white bream in liver and common perch in blood.
In the case of the micronucleus test, the highest number of micronuclei was detected in
white bream blood cells. The obtained results showed that there is no correlation between
comet assay and the micronucleus test suggesting the importance of performing different
bioassays on multiple types of fish tissues in order to find the best biomarker for assessing
the genotoxic potential of water.
PB  - Serbian Genetic Society, Belgrade, Serbia
C3  - 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia
T1  - Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2462
ER  - 

@conference{
author = "Sunjog, Karolina and Kostić, Jovana and Lakota, Ajla and Aksović, Nevena and Kolarević, Stoimir and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "This work represents the study of quality of the Danube River, in Belgrade, at the Višnjica
site, by analyzing the genotoxicity of water by using fish as bioindicators. With a population
of about 2 million inhabitants (Belgrade) and poor legislation regarding the discharge of
wastewaters, monitoring is the first step in approaching this serious problem. To obtain
information on the genotoxicity of water as a measure of DNA damage, the comet assay,
which detects the effects of exposure, and micronucleus test, which detects permanent
effect, were used. For the comet assay we have used three types of cells: blood, liver, and
gills, while the micronucleus test was done on blood cells. The fish used as bioindicators are
the common perch (Perca fluviatilis), vimba bream (Vimba vimba), common barbell (Barbus
barbus), and white bream (Blicca bjoerkna).
Regarding the comet assay, significant difference in DNA damage was observed
between species in all tested tissues. Vimba bream and common barbell had the
highest level of DNA damage in gills, white bream in liver and common perch in blood.
In the case of the micronucleus test, the highest number of micronuclei was detected in
white bream blood cells. The obtained results showed that there is no correlation between
comet assay and the micronucleus test suggesting the importance of performing different
bioassays on multiple types of fish tissues in order to find the best biomarker for assessing
the genotoxic potential of water.",
publisher = "Serbian Genetic Society, Belgrade, Serbia",
journal = "6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia",
title = "Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species",
pages = "127",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2462"
}

Sunjog, K., Kostić, J., Lakota, A., Aksović, N., Kolarević, S., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia
Serbian Genetic Society, Belgrade, Serbia., 127.
https://hdl.handle.net/21.15107/rcub_rimsi_2462

Sunjog K, Kostić J, Lakota A, Aksović N, Kolarević S, Gacic Z, Lenhardt M, Vuković-Gačić B. Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species. in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia. 2019;:127.
https://hdl.handle.net/21.15107/rcub_rimsi_2462 .

Sunjog, Karolina, Kostić, Jovana, Lakota, Ajla, Aksović, Nevena, Kolarević, Stoimir, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Ecogenotoxicological assessment of the water quality of the Danube river (site Višnjica) based on DNA damage in various fish species" in 6th Congress of the Serbian Genetic Society, Vrnjačka Banja, Serbia (2019):127,
https://hdl.handle.net/21.15107/rcub_rimsi_2462 .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1736
AB  - Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
T1  - Significance of genotoxicity and toxicity evaluation of freshwater bodies
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1736
ER  - 

@conference{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić-Vuković, Jovana and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.",
title = "Significance of genotoxicity and toxicity evaluation of freshwater bodies",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1736"
}

Sunjog, K., Kolarević, S., Kracun-Kolarevic, M., Kostić-Vuković, J., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
Institute of Marine Biology, University of Montenegro., 114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736

Sunjog K, Kolarević S, Kracun-Kolarevic M, Kostić-Vuković J, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vuković-Gačić B. Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.. 2019;:114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić-Vuković, Jovana, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Significance of genotoxicity and toxicity evaluation of freshwater bodies" in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro. (2019):114,
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1723
AB  - Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Bioassays in assessment of environmental pollution
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Bioassays in assessment of environmental pollution",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1723"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Bioassays in assessment of environmental pollution" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):115,
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1724
AB  - Untreated municipal wastewaters are one of the major negative contributors on freshwater quality, and consequently on ecosystem balance. Some compounds such as pharmaceuticals, cosmetic products, etc. could be genotoxic and lead to somatic and/or germinative mutations. This mutation should be reflected on organisms’ health and reproductive potential. Comet assay is a widely used test in ecogenotoxicology for detection of primary DNA damage. In that way, this sensitive test could provide information about early warning signs of potential stressors effects before those have an impact on the population or ecosystem level.
The scope of our study was to assess the level of DNA damage of Alburnus alburnus specimens’ gill cells and erythrocytes. Three sites on the Sava river were chosen: reference site Zabran suited upstream the municipal discharging; second at the confluence of the Kolubara river and the Sava river; and the third at the confluence of the Barička river and the Sava river. The Kolubara river and the Barička river are chosen as important recipients of untreated wastewaters. From each site, 5 bleak specimens were collected and blood and gills were taken. Tail Intensity (TI%) was chosen as a parameter for evaluating the level of DNA damage. Besides that, cell viability and extremely damaged cells - hedgehogs (HH) were determined.
The significant difference in TI% values was recorded in erythrocytes and gills cells in comparison between the Zabran (TI%=14.01±0.61 in erythrocytes, TI%=15.25±1.03 in gills cells) and the second site (TI%=18.53±0.65 in erythrocytes, TI%=22.78±1.89 in gills cells). Correlation between cell viability and HH frequency was not observed. According to the results, we could conclude that the second site is the most affected by pollutants. Also, usage of comet assay on freshwater organisms could be appropriate for preliminary screening of ecosystem state.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure
EP  - 119
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1724
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2019",
abstract = "Untreated municipal wastewaters are one of the major negative contributors on freshwater quality, and consequently on ecosystem balance. Some compounds such as pharmaceuticals, cosmetic products, etc. could be genotoxic and lead to somatic and/or germinative mutations. This mutation should be reflected on organisms’ health and reproductive potential. Comet assay is a widely used test in ecogenotoxicology for detection of primary DNA damage. In that way, this sensitive test could provide information about early warning signs of potential stressors effects before those have an impact on the population or ecosystem level.
The scope of our study was to assess the level of DNA damage of Alburnus alburnus specimens’ gill cells and erythrocytes. Three sites on the Sava river were chosen: reference site Zabran suited upstream the municipal discharging; second at the confluence of the Kolubara river and the Sava river; and the third at the confluence of the Barička river and the Sava river. The Kolubara river and the Barička river are chosen as important recipients of untreated wastewaters. From each site, 5 bleak specimens were collected and blood and gills were taken. Tail Intensity (TI%) was chosen as a parameter for evaluating the level of DNA damage. Besides that, cell viability and extremely damaged cells - hedgehogs (HH) were determined.
The significant difference in TI% values was recorded in erythrocytes and gills cells in comparison between the Zabran (TI%=14.01±0.61 in erythrocytes, TI%=15.25±1.03 in gills cells) and the second site (TI%=18.53±0.65 in erythrocytes, TI%=22.78±1.89 in gills cells). Correlation between cell viability and HH frequency was not observed. According to the results, we could conclude that the second site is the most affected by pollutants. Also, usage of comet assay on freshwater organisms could be appropriate for preliminary screening of ecosystem state.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure",
pages = "119-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1724"
}

Jovanović Marić, J., Đorđević Aleksić, J., Kolarević, S., Kračun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Paunović, M.,& Vuković-Gačić, B.. (2019). Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 118-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1724

Jovanović Marić J, Đorđević Aleksić J, Kolarević S, Kračun-Kolarević M, Kostić-Vuković J, Sunjog K, Paunović M, Vuković-Gačić B. Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:118-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1724 .

Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, Vuković-Gačić, Branka, "Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):118-119,
https://hdl.handle.net/21.15107/rcub_rimsi_1724 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Milovanović, Dragana
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Martinović, Rajko
AU  - Đorđević Aleksić, Jelena
AU  - Novaković, Irena
AU  - Sladic, Dusan
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1274
AB  - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3 '-methoxyavarone, 4 '-(methylamino)avarone and 3 '-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3 '-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3 '-methoxyavarone and 3 '-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models
EP  - 139
IS  - 2
SP  - 130
VL  - 42
DO  - 10.1080/01480545.2017.1413108
ER  - 

@article{
author = "Kolarević, Stoimir and Milovanović, Dragana and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Martinović, Rajko and Đorđević Aleksić, Jelena and Novaković, Irena and Sladic, Dusan and Vukovic-Gacic, Branka",
year = "2019",
abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3 '-methoxyavarone, 4 '-(methylamino)avarone and 3 '-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3 '-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3 '-methoxyavarone and 3 '-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models",
pages = "139-130",
number = "2",
volume = "42",
doi = "10.1080/01480545.2017.1413108"
}

Kolarević, S., Milovanović, D., Kracun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Martinović, R., Đorđević Aleksić, J., Novaković, I., Sladic, D.,& Vukovic-Gacic, B.. (2019). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor & Francis Ltd, Abingdon., 42(2), 130-139.
https://doi.org/10.1080/01480545.2017.1413108

Kolarević S, Milovanović D, Kracun-Kolarević M, Kostić-Vuković J, Sunjog K, Martinović R, Đorđević Aleksić J, Novaković I, Sladic D, Vukovic-Gacic B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2019;42(2):130-139.
doi:10.1080/01480545.2017.1413108 .

Kolarević, Stoimir, Milovanović, Dragana, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Martinović, Rajko, Đorđević Aleksić, Jelena, Novaković, Irena, Sladic, Dusan, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology, 42, no. 2 (2019):130-139,
https://doi.org/10.1080/01480545.2017.1413108 . .