TY  - JOUR
AU  - Tasić, Danica
AU  - Opačić, Miloš
AU  - Kovačević, Sanja
AU  - Nikolić-Kokić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Nikolić, Dušan
AU  - Vojnović Milutinović, Danijela
AU  - Blagojević, Duško
AU  - Djordjevic, Ana
AU  - Brkljačić, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1570
AB  - The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function
IS  - 16
SP  - 9023
VL  - 23
DO  - 10.3390/ijms23169023
ER  - 

@article{
author = "Tasić, Danica and Opačić, Miloš and Kovačević, Sanja and Nikolić-Kokić, Aleksandra and Dimitrijević, Milena and Nikolić, Dušan and Vojnović Milutinović, Danijela and Blagojević, Duško and Djordjevic, Ana and Brkljačić, Jelena",
year = "2022",
abstract = "The effects of a fructose-rich diet and chronic stress on copper metabolism in the kidneys are
still understudied. We investigated whether fructose and/or chronic unpredictable stress modulate
copper metabolism in a way that affects redox homeostasis, thus contributing to progression of
metabolic disturbances in the kidney. We determined protein level of copper transporters, chaperones,
and cuproenzymes including cytochrome c oxidase, as well as antioxidant enzymes function in the
kidneys of male Wistar rats subjected to 20% liquid fructose supplementation and/or chronic stress.
Liquid fructose supplementation increased level of copper chaperone of superoxide dismutase and
decreased metallothionein level, while rendering the level of copper importer and copper chaperones
involved in copper delivery to mitochondria and trans Golgi network unaffected. Stress had no effect
on renal copper metabolism. The activity and expression of renal antioxidant enzymes remained
unaltered in all experimental groups. In conclusion, fructose, independently of stress, decreased
renal copper level, and modulated renal copper metabolism as to preserve vital cellular function
including mitochondrial energy production and antioxidative defense, at the expense of intracellular
copper storage.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function",
number = "16",
pages = "9023",
volume = "23",
doi = "10.3390/ijms23169023"
}

Tasić, D., Opačić, M., Kovačević, S., Nikolić-Kokić, A., Dimitrijević, M., Nikolić, D., Vojnović Milutinović, D., Blagojević, D., Djordjevic, A.,& Brkljačić, J.. (2022). Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences
MDPI., 23(16), 9023.
https://doi.org/10.3390/ijms23169023

Tasić D, Opačić M, Kovačević S, Nikolić-Kokić A, Dimitrijević M, Nikolić D, Vojnović Milutinović D, Blagojević D, Djordjevic A, Brkljačić J. Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function. in International Journal of Molecular Sciences. 2022;23(16):9023.
doi:10.3390/ijms23169023 .

Tasić, Danica, Opačić, Miloš, Kovačević, Sanja, Nikolić-Kokić, Aleksandra, Dimitrijević, Milena, Nikolić, Dušan, Vojnović Milutinović, Danijela, Blagojević, Duško, Djordjevic, Ana, Brkljačić, Jelena, "Effects of Fructose and Stress on Rat Renal Copper Metabolism and Antioxidant Enzymes Function" in International Journal of Molecular Sciences, 23, no. 16 (2022):9023,
https://doi.org/10.3390/ijms23169023 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Vojnović Milutinović, Danijela
AU  - Jovanović-Marić, Jovana
AU  - K.T. Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1566
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters
SP  - 156964
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Vojnović Milutinović, Danijela and Jovanović-Marić, Jovana and K.T. Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukic, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters",
pages = "156964",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Vojnović Milutinović, D., Jovanović-Marić, J., K.T. Kirschner, A., Farnleitner, A., Linke, R., Đukic, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment
Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Vojnović Milutinović D, Jovanović-Marić J, K.T. Kirschner A, Farnleitner A, Linke R, Đukic A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Vojnović Milutinović, Danijela, Jovanović-Marić, Jovana, K.T. Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukic, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters" in Science of the Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .