TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .