TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szanto-Egesz, Reka
AU  - Lukacs, Alena
AU  - Kracun-Kolarević, Margareta
AU  - Lundy, Lian
AU  - Kirschner, Alexander K. T.
AU  - Farnleitner, Andreas
AU  - Djukic, Aleksandar
AU  - Colic, Jasna
AU  - Nenin, Tanja
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1489
AB  - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nudeocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 x 10(3) to 132 x 10(4) copies,1) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
VL  - 783
DO  - 10.1016/j.scitotenv.2021.146967
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szanto-Egesz, Reka and Lukacs, Alena and Kracun-Kolarević, Margareta and Lundy, Lian and Kirschner, Alexander K. T. and Farnleitner, Andreas and Djukic, Aleksandar and Colic, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir",
year = "2021",
abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nudeocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 x 10(3) to 132 x 10(4) copies,1) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters",
volume = "783",
doi = "10.1016/j.scitotenv.2021.146967"
}

Kolarević, S., Micsinai, A., Szanto-Egesz, R., Lukacs, A., Kracun-Kolarević, M., Lundy, L., Kirschner, A. K. T., Farnleitner, A., Djukic, A., Colic, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment
Elsevier, Amsterdam., 783.
https://doi.org/10.1016/j.scitotenv.2021.146967

Kolarević S, Micsinai A, Szanto-Egesz R, Lukacs A, Kracun-Kolarević M, Lundy L, Kirschner AKT, Farnleitner A, Djukic A, Colic J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment. 2021;783.
doi:10.1016/j.scitotenv.2021.146967 .

Kolarević, Stoimir, Micsinai, Adrienn, Szanto-Egesz, Reka, Lukacs, Alena, Kracun-Kolarević, Margareta, Lundy, Lian, Kirschner, Alexander K. T., Farnleitner, Andreas, Djukic, Aleksandar, Colic, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of the Total Environment, 783 (2021),
https://doi.org/10.1016/j.scitotenv.2021.146967 . .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Jovanović, J.
AU  - Ilić, M.
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, S.
AU  - Joksimović, D.
AU  - Pešić, V.
AU  - Kirschner, A.K.T.
AU  - Linke, R.
AU  - Ixenmaier, S.
AU  - Farnleitner, Andreas
AU  - Savio, D.
AU  - Reischer, Georg
AU  - Tomić, N.
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1310
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Microbiological water quality of rivers in Montenegro
EP  - 155
SP  - 135
VL  - 93
DO  - 10.1007/698_2019_420
ER  - 

@inbook{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Jovanović, J. and Ilić, M. and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, S. and Joksimović, D. and Pešić, V. and Kirschner, A.K.T. and Linke, R. and Ixenmaier, S. and Farnleitner, Andreas and Savio, D. and Reischer, Georg and Tomić, N. and Vukovic-Gacic, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbiological water quality of rivers in Montenegro",
pages = "155-135",
volume = "93",
doi = "10.1007/698_2019_420"
}

Kolarević, S., Kracun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vukovic-Gacic, B.. (2020). Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 135-155.
https://doi.org/10.1007/698_2019_420

Kolarević S, Kracun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner A, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vukovic-Gacic B. Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry. 2020;93:135-155.
doi:10.1007/698_2019_420 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Jovanović, J., Ilić, M., Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, Andreas, Savio, D., Reischer, Georg, Tomić, N., Vukovic-Gacic, Branka, "Microbiological water quality of rivers in Montenegro" in Handbook of Environmental Chemistry, 93 (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Milosković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1007
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Simonović, Predrag and Simic, Vladica and Milosković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450"
}

Kolarević, S., Aborgiba, M., Kracun-Kolarević, M., Kostić-Vuković, J., Simonović, P., Simic, V., Milosković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kracun-Kolarević M, Kostić-Vuković J, Simonović P, Simic V, Milosković A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Simonović, Predrag, Simic, Vladica, Milosković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .

TY  - JOUR
AU  - Vrzel, Janja
AU  - Vukovic-Gacic, Branka
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Aborgiba, Mustafa
AU  - Farnleitner, Andreas
AU  - Reischer, Georg
AU  - Linke, Rita
AU  - Paunović, Momir
AU  - Ogrinc, Nives
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1016
AB  - Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin
EP  - 1471
SP  - 1460
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.213
ER  - 

@article{
author = "Vrzel, Janja and Vukovic-Gacic, Branka and Kolarević, Stoimir and Gačić, Zoran and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Ogrinc, Nives",
year = "2016",
abstract = "Coupled measurements of nitrate (NO3-), nitrogen (N), and oxygen (O) isotopic composition (delta N-15(NO3) and delta O-18(NO3)) were used to investigate the sources and processes of N cycling, while the microbial source tracking (MST) method was used to identify microbiological pollution in the surface water of the Sava River Basin (SRB) in autumn in 2014 and 2015 during high and low water discharge. Atmospheric nitrate deposition or nitrate-containing fertilizers were found not to be significant sources of riverine nitrate in the SRB. The ranges of isotope values suggest that NO3- in the SRB derives from soil nitrification, sewage, and/or manure, which were further supported by MST analysis. Microbiological indicators show the existence of hotspots of fecal pollution in the SRB, which are human associated. Long-term observations indicate persistent fecal contamination at selected locations caused by continuous discharge of untreated wastewaters into the SRB.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin",
pages = "1471-1460",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.213"
}

Vrzel, J., Vukovic-Gacic, B., Kolarević, S., Gačić, Z., Kracun-Kolarević, M., Kostić-Vuković, J., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Ogrinc, N.. (2016). Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1460-1471.
https://doi.org/10.1016/j.scitotenv.2016.07.213

Vrzel J, Vukovic-Gacic B, Kolarević S, Gačić Z, Kracun-Kolarević M, Kostić-Vuković J, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Ogrinc N. Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin. in Science of the Total Environment. 2016;573:1460-1471.
doi:10.1016/j.scitotenv.2016.07.213 .

Vrzel, Janja, Vukovic-Gacic, Branka, Kolarević, Stoimir, Gačić, Zoran, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Ogrinc, Nives, "Determination of the sources of nitrate and the microbiological sources of pollution in the Sava River Basin" in Science of the Total Environment, 573 (2016):1460-1471,
https://doi.org/10.1016/j.scitotenv.2016.07.213 . .