TY  - JOUR
AU  - Vuković-Gačić, Branka
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Joksimović, Danijela
AU  - Martinović, Rajko
AU  - Ramšak, Andreja
AU  - Bajt, Oliver
AU  - Đorđević Aleksić, Jelena
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2160
AB  - In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)
EP  - 12
IS  - 38
SP  - 3
VL  - 1
DO  - 10.1093/mutage/geac017
ER  - 

@article{
author = "Vuković-Gačić, Branka and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Joksimović, Danijela and Martinović, Rajko and Ramšak, Andreja and Bajt, Oliver and Đorđević Aleksić, Jelena",
year = "2023",
abstract = "In this study, the possible ‘vector effect’ within the exposure of Mediterranean mussels (Mytilus galloprovincialis) to polystyrene microplastics with adsorbed fluoranthene was investigated by applying the multibiomarker approach. The major focus was placed on genotoxicological endpoints as to our knowledge there are no literature data on the genotoxicity of polystyrene microparticles alone or with adsorbed fluoranthene in the selected experimental organisms. DNA damage was assessed in haemocytes by comet assay and micronucleus test. For the assess- ment of neurotoxicity, acetylcholinesterase activity was measured in gills. Glutathione S-transferase was assessed in gills and hepatopancreas since these enzymes are induced for biotransformation and excretion of lipophilic compounds such as hydrocarbons. Finally, differences in physiological response within the exposure to polystyrene particles, fluoranthene, or particles with adsorbed fluoranthene were assessed by the variation of heart rate patterns studied by the noninvasive laser fibre-optic method. The uniform response of individual biomarkers within the exposure groups was not recorded. There was no clear pattern in variation of acetylcholinesterase or glutathione S-transferase activity which could be attributed to the treatment. Exposure to polystyrene increased DNA damage which was detected by the comet assay but was not confirmed by micronucleus formation. Data of genotoxicity assays indicated differential responses among the groups exposed to fluoranthene alone and fluoranthene adsorbed to polystyrene. Change in the heart rate patterns within the studied groups supports the concept of the Trojan horse effect within the exposure to polystyrene particles with adsorbed fluoranthene.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)",
pages = "12-3",
number = "38",
volume = "1",
doi = "10.1093/mutage/geac017"
}

Vuković-Gačić, B., Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Joksimović, D., Martinović, R., Ramšak, A., Bajt, O.,& Đorđević Aleksić, J.. (2023). Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis
Oxford University Press., 1(38), 3-12.
https://doi.org/10.1093/mutage/geac017

Vuković-Gačić B, Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Joksimović D, Martinović R, Ramšak A, Bajt O, Đorđević Aleksić J. Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis). in Mutagenesis. 2023;1(38):3-12.
doi:10.1093/mutage/geac017 .

Vuković-Gačić, Branka, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Joksimović, Danijela, Martinović, Rajko, Ramšak, Andreja, Bajt, Oliver, Đorđević Aleksić, Jelena, "Single and combined potential of polystyrene microparticles and fluoranthene in the induction of DNA damage in haemocytes of Mediterranean mussel (Mytilus galloprovincialis)" in Mutagenesis, 1, no. 38 (2023):3-12,
https://doi.org/10.1093/mutage/geac017 . .

TY  - JOUR
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Nikolić, Ivan
AU  - Marić, Ana
AU  - Ilić, Marija
AU  - Simonović, Predrag
AU  - Alygizakis, Nikiforos
AU  - Ng, Kilsey
AU  - Oswald, Petar
AU  - Slobodnik, Jaroslav
AU  - Žegura, Bojana
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
AU  - Kračun-Kolarević, Margareta
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2044
AB  - Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.
PB  - Oxford University Press
T2  - Mutagenesis
T1  - In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study
EP  - 32
IS  - 1
SP  - 21
VL  - 38
DO  - 10.1093/mutage/geac024
ER  - 

@article{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Nikolić, Ivan and Marić, Ana and Ilić, Marija and Simonović, Predrag and Alygizakis, Nikiforos and Ng, Kilsey and Oswald, Petar and Slobodnik, Jaroslav and Žegura, Bojana and Vuković-Gačić, Branka and Paunović, Momir and Kračun-Kolarević, Margareta",
year = "2023",
abstract = "Environmental studies which aim to assess the ecological impact of chemical and other types of pollution should employ a complex weight-of-evidence approach with multiple lines of evidence (LoEs). This study focused on in situ genotoxicological methods such as the comet and micronucleus assays and randomly amplified polymorphic DNA analysis as one of the multiple LoEs (LoE3) on the fish species Alburnus alburnus (bleak) as a bioindicator. The study was carried out within the Joint Danube Survey 4 (JDS4) at nine sites in the Danube River Basin in the Republic of Serbia. Out of nine sampling sites, two were situated at the Tisa, Sava, and Velika Morava rivers, and three sites were at the Danube River. The three additionally employed LoEs were: SumTUwater calculated based on the monitoring data in the database of the Serbian Environmental Protection Agency (SEPA) (LoE1); in vitro analyses of JDS4 water extracts employing genotoxicological methods (LoE2); assessment of the ecological status/potential by SEPA and indication of the ecological status for the sites performed within the JDS4 (LoE4). The analyzed biomarker responses in the bleak were integrated into the unique integrated biomarker response index which was used to rank the sites. The highest pollution pressure was recorded at JDS4 39 and JDS4 36, while the lowest was at JDS4 35. The impact of pollution was confirmed at three sites, JDS4 33, 40, and 41, by all four LoEs. At other sampling sites, a difference was observed regarding the pollution depending on the employed LoEs. This indicates the importance of implementing a comprehensive weight-of-evidence approach to ensure the impact of pollution is not overlooked when using only one LoE as is often the case in environmental studies.",
publisher = "Oxford University Press",
journal = "Mutagenesis",
title = "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study",
pages = "32-21",
number = "1",
volume = "38",
doi = "10.1093/mutage/geac024"
}

Jovanović Marić, J., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Nikolić, I., Marić, A., Ilić, M., Simonović, P., Alygizakis, N., Ng, K., Oswald, P., Slobodnik, J., Žegura, B., Vuković-Gačić, B., Paunović, M.,& Kračun-Kolarević, M.. (2023). In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis
Oxford University Press., 38(1), 21-32.
https://doi.org/10.1093/mutage/geac024

Jovanović Marić J, Kolarević S, Đorđević Aleksić J, Sunjog K, Nikolić I, Marić A, Ilić M, Simonović P, Alygizakis N, Ng K, Oswald P, Slobodnik J, Žegura B, Vuković-Gačić B, Paunović M, Kračun-Kolarević M. In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study. in Mutagenesis. 2023;38(1):21-32.
doi:10.1093/mutage/geac024 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Nikolić, Ivan, Marić, Ana, Ilić, Marija, Simonović, Predrag, Alygizakis, Nikiforos, Ng, Kilsey, Oswald, Petar, Slobodnik, Jaroslav, Žegura, Bojana, Vuković-Gačić, Branka, Paunović, Momir, Kračun-Kolarević, Margareta, "In situ detection of the genotoxic potential as one of the lines of evidence in the weight-of-evidence approach—the Joint Danube Survey 4 Case Study" in Mutagenesis, 38, no. 1 (2023):21-32,
https://doi.org/10.1093/mutage/geac024 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1729
AB  - The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries
SP  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1729
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "The middle section of the Danube River basin in the previous three Joint Danube Surveys (JDS) was found to be under intensive pollution, mainly affected by wastewaters. As a consequence of the lack of wastewater treatment plants, complex xenobiotics mixtures may get into surface water
and decrease water quality and organisms’ health. Some xenobiotics may induce different types of DNA damage. Hence, DNA damage could be a useful biomarker in the detection of “early warning signals” of organisms’ exposure to genotoxic compounds, while the usage of bioassays batteries
can provide better insight into a genotoxic potential. This study was conducted in 2019, during the JDS4 expedition, to assess the genotoxic potential of nine sites on the section of the Danube River which goes through Serbia and its major tributaries: the Sava, Tisza and Velika Morava. Blood and muscle of Alburnus alburnus (bleak) were used for genotoxicity assessment by comet, micronucleus and RAPD assays. In comet assay, the highest level of DNA damage was recorded at the Velika Morava mouth (JDS 39) and on the Danube River, downstream Radujevac (JDS 41). The highest frequency of micronucleus was recorded at the Sava mouth (JDS 36) and on the Danube River, downstream Pančevo (JDS 37). The lowest level of DNA damage in both tests was recorded on the Sava, site Jamena (JDS 35). The RAPD analysis distinct three major groups: Tisza mouth (JDS 33) and sites on the Sava (JDS 35, JDS 36), then two sites on the Danube River (JDS 37, JDS 41) and one site on the Danube River, Ram (JDS 40) with the Velika Morava mouth (JDS 39). Analyzed bioassays showed different sensitivity where the comet assay had the highest potential in discrimination of sites based on genotoxicity. Bleak was proved to be a reliable bioindicator in eco/ genotoxicological studies.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries",
pages = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1729"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:69.
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Evaluation of genotoxic potential of the middle section of the Danube River and its major tributaries" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):69,
https://hdl.handle.net/21.15107/rcub_rimsi_1729 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1725
AB  - Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
T1  - Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research
SP  - 42
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1725
ER  - 

@conference{
author = "Kolarević, Stoimir and Kračun-Kolarević, Margareta and Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2022",
abstract = "Within the past decade, our research group placed great effort in exploration of the impact of treated/untreated wastewaters on aquatic ecosystems as less than 13 % of collected municipal wastewaters are processed before their release to receiving waters in Serbia. Having in mind that more than 90% of the rivers within the territory of Serbia belong to the Danube River Basin, our major focus is naturally on the large transnational waters such as the Danube and Sava rivers. Depended on the concept of the study and hydro-morphological characteristics of the sites, various approaches have been employed for in situ assessment of eco/geno-toxicity which use different organisms in passive and active biomonitoring. Aquatic organisms of different trophic levels have diverse life strategies, metabolism pathways, and consequently, they have a different response to pollutant pressure. In eco/geno-toxicological studies we have successfully employed several species of freshwater mussels (Unio tumidus, Unio pictorum and Sinanodonta woodiana) as well as few economically important fish species (common carp (Cyprinus carpio), freshwater bream (Abramis brama), bleak (Alburnus alburnus, chub (Squalius cephalus)).Comet assay is our primary choice for the assessment of DNA damage considering its sensitivity and cost effectiveness. Selection of certain types of tissues for the assessment (such as blood or haemolymph), which do not require sacrificing of the animals or additional manipulation in preparation of cell suspension coupled with mini-gel format of the slides enables high-throughput screening of the genotoxic potential in situ. Additionally, cryopreservation of the blood samples directly onsite expands the research area as it overcomes issues related to safe transportation of the samples to the laboratory. Data obtained so far indicated that the genotoxic response in the studied indicator organisms significantly differ which is understandable considering the difference in uptake, accumulation and physiological responses.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)",
title = "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research",
pages = "42",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1725"
}

Kolarević, S., Kračun-Kolarević, M., Jovanović Marić, J., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Gačić, Z.,& Vuković-Gačić, B.. (2022). Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS)
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725

Kolarević S, Kračun-Kolarević M, Jovanović Marić J, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Gačić Z, Vuković-Gačić B. Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research. in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS). 2022;:42.
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

Kolarević, Stoimir, Kračun-Kolarević, Margareta, Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Vuković-Gačić, Branka, "Application of comet assay in aquatic organisms – summary and lessons learned in past 10 years of field research" in 14th International Comet Assay Workshop (ICAW) & 50th meeting of the European Environmental Mutagen and Genomics Society (EEMGS) (2022):42,
https://hdl.handle.net/21.15107/rcub_rimsi_1725 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2166
AB  - У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.
PB  - Народна библиотека Србије
C3  - Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
T1  - Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2166
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana and Paunović, Momir and Vuković-Gačić, Branka",
year = "2022",
abstract = "У Србији се прерађује мање од 13% отпадних вода,1 што за последицу има испуштање великих количина непречишћених вода у водотокове. Равничарске реке су под значајним притисцима ових извора загађења, с обзиром да делом или читавим током протичу кроз насељене области, као и области у којима се налазе индустријска постројења. У анализи утицаја загађења на акватичне организме, оштећење ДНК молекула представља значајан биомаркер раног упозорења јер може указати на утицај ксенобиотика на биолошке системе пре него што се одрази на читав организам и/или популацију. У складу са тим, циљ овог истраживања је процена осетљивости широко распрострањене врсте Alburnus alburnus (уклија) приликом детекције генотоксичног ефекта, како на просторно блиским локалитетима на Сави, тако на и просторно удаљеним на Дунаву, Тиси, Сави и Великој Морави. За поређење локалитета коришћен је интегрисани одговор биомаркера (ИБР) који је објединио појединачне биомаркере: вијабилност ћелија крви, ниво ДНК оштећења ћелија крви детектованог у алкалном комет и микронуклеус тесту и индекс загађења металима/приоритетним супстанцама. Интегрисањем појединачних одговора биомаркера у јединствену ИБР вредност омогућено је једноставније поређење локалитета. Добијени резултати су указали да су одговори биомаркера уклија специфични за локалитет. У складу са резултатима, утврђено је да уклија може бити поуздан биоиндикатор у дискриминацији и удаљених и просторно блиских локалитета који су под различитим утицајем загађења.",
publisher = "Народна библиотека Србије",
journal = "Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia",
title = "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2166"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K., Kostić-Vuković, J., Paunović, M.,& Vuković-Gačić, B.. (2022). Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia
Народна библиотека Србије., 155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J, Paunović M, Vuković-Gačić B. Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka. in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, Paunović, Momir, Vuković-Gačić, Branka, "Ispitivanje osetljivosti vrste Alburnus alburnus (uklija) u ekogenotoksikološkim istraživanjima velikih ravničarskih reka" in Treći kongres biologa Srbije, Srpsko biološko društvo, 21-25 September 2022, Zlatibor, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_rimsi_2166 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2164
AB  - Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.
PB  - Prirodno-matematički fakultet, UNS
C3  - 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
T1  - Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2164
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Sunjog, Karolina and Kostić-Vuković, Jovana",
year = "2022",
abstract = "Velika, Zapadna, Južna Morava, kao i Sava, Kolubara i Barička reka su pod značajnim uticajem komunalnih i industrijskih otpadnih voda. Ciljevi studije su procena genotoksičnog potencijala analiziranih lokaliteta i ispitivanje osetljivosti primenjenih biotestova i pristupa istraživanja. Studija slučaja Velika Morava obuhvatila je veliko ispitivano područje na osam lokaliteta na Velikoj, Zapadnoj i Južnoj Moravi, a u studiji Sava ispitivano je tri prostorno bliska lokaliteta koji su pod različitim pritiscima zagađenja. U in silico pristupu na osnovu literaturnih podataka analiziran je toksični/genotoksični potencijal jedinjenja detektovanih u vodi. Ex situ/in vitro pristup obuhvatio je ispitivanje genotoksičnog potencijala pomoću SOS/umuC testa, kao i primenom alkalnog komet testa na HepG2 ćelijskoj liniji. U in situ/in vivo istraživanju analizirano je DNK oštećenje ćelija krvi vrste Alburnus alburnus (uklija) pomoću alkalnog, Fpg-modifikovanog komet testa i mikronukleus testa. Rezultati su pokazali različitu osetljivost zavisno od upotrebljenih testova i pristupa, ali i različit odgovor kod uklija u zavisnosti od tipa zagađenja na lokalitetima. In silico i ex situ/in vitro pristupi su pokazali niži stepen osetljivosti u odnosu na in situ/in vivo testove, a alkalni komet test je pokazao najveći potencijal u diskriminaciji lokaliteta. Utvrđeno je da uklija može biti pouzdan bioindikator u ekogenotoksikološkim istraživanjima velikih ravničarskih reka.",
publisher = "Prirodno-matematički fakultet, UNS",
journal = "9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.",
title = "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2164"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Sunjog, K.,& Kostić-Vuković, J.. (2022). Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.
Prirodno-matematički fakultet, UNS., 2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Sunjog K, Kostić-Vuković J. Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava. in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia.. 2022;2.
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Sunjog, Karolina, Kostić-Vuković, Jovana, "Ispitivanje osetljivosti testova i pristupa u ekogenotoksikološkim istraživanjima na velikim ravničarskim rekama–studije slučaja Velika Morava i Sava" in 9. memorijalni naučni skup iz zaštite životne sredine" Docent dr Milena Dalmacija": 2022 Mar 31-Apr 1; Novi Sad, Serbia., 2 (2022),
https://hdl.handle.net/21.15107/rcub_rimsi_2164 .

TY  - CONF
AU  - Martinović, Rajko
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
AU  - Perošević-Bajčeta, Ana
AU  - Joksimović, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5726
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2172
AB  - More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.
PB  - Institute of Oceanography and Fisheries
C3  - 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
T1  - Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2172
ER  - 

@conference{
author = "Martinović, Rajko and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Gačić, Zoran and Vuković-Gačić, Branka and Perošević-Bajčeta, Ana and Joksimović, Danijela",
year = "2022",
abstract = "More frequent application of biological parameters beside the conventional chemical parameters in national monitoring for marine ecosystem assessment is supported by UNEP/MAP. Accordingly, to evaluate the possible effects of pollution by determination of trace element content and genotoxicity assessment in marine organisms within the Boka Kotorska Bay, Montenegro (southern Adriatic), we carried a field study which included transplantation of the seabream (Sparus aurata) from aquaculture farm (Orahovac) to more impacted sites (Dobrota and port of Tivat), situated in vicinity of the main ports. On the sampling site Orahovac, the group of seabream specimens were placed in closed fish trap on 5 m depth (O1), another fish trap was placed in Dobrota, while the third fish trap with seabreams was placed at the site port of Tivat. The additional group was sampled from standard aquaculture cage on sampling site (O2) to reduce possible bias in genotoxicity data due to confined space within the fish trap. After two weeks of exposure, blood from fish heart was taken, afterwards muscle tissues were prepared for trace element determination. Genotoxicity was measured by DNA damage induction based on comet (single cell gel electrophoresis) assay parameter – Tail intensity (TI%), while trace element (As, Cd, Pb, Hg, Cr, Cu, Fe, Mn, Ni, Zn and V) content was determined by ICP. Mean values of TI% obtained from seabream specimens at the sampling site Orahovac were: 4.26 ± 0.17 – O1 and 2.56 ± 0.11 – O2, while TI% values for transplanted specimens on the sites Dobrota and port of Tivat were 6.38 ± 0.17 and 11.06 ± 0.23, respectively. Statistically significant differences (p<0,05) of TI% were observed between all specimens groups. The group of seabream from port of Tivat showed the highest TI% (higher level of DNA damage), most probably caused by marine pollution. Obtained trace element concentrations in seabream tissues were significantly lower in comparison to values recommended by FAO, EC and national legislatives of Croatia, Spain and Turkey. Since transplanted fish showed a significant response in a relatively short exposure period, our results support the introduction of seabream caging as monitoring tool to reveal marine pollution by multiple biomarker approach. Considering the significant share of aquacultured seabream in human consumption, higher availability for sampling and convenience in application for molecular biomarker analyses in the same samples, such an integrated monitoring approach would contribute to more credible data.",
publisher = "Institute of Oceanography and Fisheries",
journal = "2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries",
title = "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2172"
}

Martinović, R., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Gačić, Z., Vuković-Gačić, B., Perošević-Bajčeta, A.,& Joksimović, D.. (2022). Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries
Institute of Oceanography and Fisheries., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172

Martinović R, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Gačić Z, Vuković-Gačić B, Perošević-Bajčeta A, Joksimović D. Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast. in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries. 2022;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

Martinović, Rajko, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Gačić, Zoran, Vuković-Gačić, Branka, Perošević-Bajčeta, Ana, Joksimović, Danijela, "Transplant caging of seabream (Sparus aurata) as a monitoring tool for marine pollution assessment in the Montenegrin Adriatic coast" in 2nd Southeast European Ichthyological Conference (SEEIC); 2022 Oct 12-15; Brač Island, Croatia. Split: Institute of Oceanography and Fisheries (2022):33,
https://hdl.handle.net/21.15107/rcub_rimsi_2172 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Jovanović Marić, Jovana
AU  - Vojnović Milutinović, Danijela
AU  - Kirschner, Alexander K. T.
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Djukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1920
AB  - Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.
PB  - Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)
C3  - FEMS Conference on Microbiology in association with Serbian Society of Microbiology
T1  - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
SP  - 614
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1920
ER  - 

@conference{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Jovanović Marić, Jovana and Vojnović Milutinović, Danijela and Kirschner, Alexander K. T. and Farnleitner, Andreas and Linke, Rita and Djukic, Aleksandar and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir",
year = "2022",
abstract = "Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of  SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.",
publisher = "Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)",
journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology",
title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters",
pages = "614",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1920"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Jovanović Marić, J., Vojnović Milutinović, D., Kirschner, A. K. T., Farnleitner, A., Linke, R., Djukic, A., Kostić-Vuković, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology
Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)., 614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Jovanović Marić J, Vojnović Milutinović D, Kirschner AKT, Farnleitner A, Linke R, Djukic A, Kostić-Vuković J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:614.
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Jovanović Marić, Jovana, Vojnović Milutinović, Danijela, Kirschner, Alexander K. T., Farnleitner, Andreas, Linke, Rita, Djukic, Aleksandar, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):614,
https://hdl.handle.net/21.15107/rcub_rimsi_1920 .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Djordjevic, Ana
AU  - Vojnović Milutinović, Danijela
AU  - Jovanović-Marić, Jovana
AU  - K.T. Kirschner, Alexander
AU  - Farnleitner, Andreas
AU  - Linke, Rita
AU  - Đukic, Aleksandar
AU  - Kostić-Vuković, Jovana
AU  - Paunović, Momir
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1566
AB  - Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters
SP  - 156964
VL  - 843
DO  - 10.1016/j.scitotenv.2022.156964
ER  - 

@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Vojnović Milutinović, Danijela and Jovanović-Marić, Jovana and K.T. Kirschner, Alexander and Farnleitner, Andreas and Linke, Rita and Đukic, Aleksandar and Kostić-Vuković, Jovana and Paunović, Momir",
year = "2022",
abstract = "Wastewater-based epidemiology (WBE) surveillance of COVID-19 and other future outbreaks is a challenge for developing countries as most households are not connected to a sewerage system. In December 2019, SARS-CoV-2 RNA was detected in the Danube River at a site severely affected by wastewaters from Belgrade. Rivers are much more complex systems than wastewater systems, and efforts are needed to address all the factors influencing the adoption of WBE as an alternative to targeting raw wastewater. Our objective was to provide a more detailed insight into the potential of SARS-CoV-2 surveillance in Serbian surface waters for epidemiological purposes. Water samples were collected at 12 sites along the Sava and Danube rivers in Belgrade during the fourth COVID-19 wave in Serbia that started in late February 2021. RNA was concentrated using Amicon Ultra-15 centrifugal filters and quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (faecal indicator bacteria and human and animal genetic faecal source tracking markers), epidemiological, physicochemical and hydromorphological parameters were analysed in parallel. From 44 samples, SARS-CoV-2 RNA was detected in 31, but only at 4 concentrations above the level of quantification (ranging from 8.47 × 103 to 2.07 × 104 gc/L). The results indicated that surveillance of SARS-CoV-2 RNA in surface waters as ultimate recipients could be used as an epidemiological early-warning tool in countries lacking wastewater treatment and proper sewerage infrastructure. The performance of the applied approach, including advanced sampling site characterization to trace and identify sites with significant raw sewage influence from human populations, could be further improved by adaptation of the methodology for processing higher volumes of samples and enrichment factors, which should provide the quantitative instead of qualitative data needed for WBE.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters",
pages = "156964",
volume = "843",
doi = "10.1016/j.scitotenv.2022.156964"
}

Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Vojnović Milutinović, D., Jovanović-Marić, J., K.T. Kirschner, A., Farnleitner, A., Linke, R., Đukic, A., Kostić-Vuković, J.,& Paunović, M.. (2022). Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment
Elsevier., 843, 156964.
https://doi.org/10.1016/j.scitotenv.2022.156964

Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Vojnović Milutinović D, Jovanović-Marić J, K.T. Kirschner A, Farnleitner A, Linke R, Đukic A, Kostić-Vuković J, Paunović M. Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters. in Science of the Total Environment. 2022;843:156964.
doi:10.1016/j.scitotenv.2022.156964 .

Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Vojnović Milutinović, Danijela, Jovanović-Marić, Jovana, K.T. Kirschner, Alexander, Farnleitner, Andreas, Linke, Rita, Đukic, Aleksandar, Kostić-Vuković, Jovana, Paunović, Momir, "Wastewater-based epidemiology in countries with poor wastewater treatment — Epidemiological indicator function of SARS-CoV-2 RNA in surface waters" in Science of the Total Environment, 843 (2022):156964,
https://doi.org/10.1016/j.scitotenv.2022.156964 . .

TY  - CONF
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović Marić, Jovana
AU  - Vuković-Gačić, Branka
AU  - Duletic-Lausevic, Sonja
AU  - Marin, Petar D.
AU  - Božić Nedeljković, Biljana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2176
AB  - Recently, cancer research has focused on searching for new and more effective antitumor agents of natural origin that can activate multiple defence mechanisms and selectively damage transformed cells. The goal of this research was to assess different antitumor mechanisms of ethanolic extracts of 18 Lamiaceae species traditionally used in Serbian folk medicine and cuisine, as well as their genotoxic potential towards HCT-116 (colorectal cancer) cells. The viability of treated HCT-116 cells was assessed by MTT assay; the production of reactive oxygen species (ROS) by treated HCT- 116 cells was determined using NBT assay, while their production of nitric oxide (NO) was evaluated using Griess assay. The genotoxic activity of the extracts on HCT-116 cells was tested in Comet assay, using etoposide as a positive control. The results indicated that lavender, basil, and rosemary inhibited the proliferation of these cells, significantly lowering their viability. Moreover, lavender and thyme extracts displayed a significant increase in ROS production, whereas ground- ivy, hyssop, lemon balm, peppermint, basil, rosemary, sage, and winter savory have significantly lowered their production. The results of the Griess assay suggested that lavender, motherwort, peppermint, basil, rosemary, sage, winter savory, ironwort, and thyme have significantly increased the production of NO. Furthermore, Comet assay results pointed out that motherwort, peppermint, basil, oregano, marjoram, winter savory, ironwort, wild thyme, thyme, and mountain germander possess genotoxic potential towards HCT-116 cells, while only basil had genotoxic activity statistically similar to etoposide. The obtained results are in accordance with our previous findings, which indicated that these extracts have antigenotoxic and genoprotective activities towards normal cells. Finally, it can be concluded that these traditionally valued plants might act as potent antitumor agents by modulating the proliferation and production of ROS and NO by cancer cells, as well as by expressing significant genotoxic properties towards cancer cells.
PB  - University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)
C3  - 2nd International UNIFood Conference, September 24-25, Belgrade
T1  - Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2176
ER  - 

@conference{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Jovanović Marić, Jovana and Vuković-Gačić, Branka and Duletic-Lausevic, Sonja and Marin, Petar D. and Božić Nedeljković, Biljana",
year = "2021",
abstract = "Recently, cancer research has focused on searching for new and more effective antitumor agents of natural origin that can activate multiple defence mechanisms and selectively damage transformed cells. The goal of this research was to assess different antitumor mechanisms of ethanolic extracts of 18 Lamiaceae species traditionally used in Serbian folk medicine and cuisine, as well as their genotoxic potential towards HCT-116 (colorectal cancer) cells. The viability of treated HCT-116 cells was assessed by MTT assay; the production of reactive oxygen species (ROS) by treated HCT- 116 cells was determined using NBT assay, while their production of nitric oxide (NO) was evaluated using Griess assay. The genotoxic activity of the extracts on HCT-116 cells was tested in Comet assay, using etoposide as a positive control. The results indicated that lavender, basil, and rosemary inhibited the proliferation of these cells, significantly lowering their viability. Moreover, lavender and thyme extracts displayed a significant increase in ROS production, whereas ground- ivy, hyssop, lemon balm, peppermint, basil, rosemary, sage, and winter savory have significantly lowered their production. The results of the Griess assay suggested that lavender, motherwort, peppermint, basil, rosemary, sage, winter savory, ironwort, and thyme have significantly increased the production of NO. Furthermore, Comet assay results pointed out that motherwort, peppermint, basil, oregano, marjoram, winter savory, ironwort, wild thyme, thyme, and mountain germander possess genotoxic potential towards HCT-116 cells, while only basil had genotoxic activity statistically similar to etoposide. The obtained results are in accordance with our previous findings, which indicated that these extracts have antigenotoxic and genoprotective activities towards normal cells. Finally, it can be concluded that these traditionally valued plants might act as potent antitumor agents by modulating the proliferation and production of ROS and NO by cancer cells, as well as by expressing significant genotoxic properties towards cancer cells.",
publisher = "University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)",
journal = "2nd International UNIFood Conference, September 24-25, Belgrade",
title = "Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.",
pages = "63",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2176"
}

Oalđe Pavlović, M., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Jovanović Marić, J., Vuković-Gačić, B., Duletic-Lausevic, S., Marin, P. D.,& Božić Nedeljković, B.. (2021). Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.. in 2nd International UNIFood Conference, September 24-25, Belgrade
University of Belgrade, 2021 (Beograd : Razvojno-istraživački centar Grafičkog inženjerstva TMF)., 63.
https://hdl.handle.net/21.15107/rcub_rimsi_2176

Oalđe Pavlović M, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Jovanović Marić J, Vuković-Gačić B, Duletic-Lausevic S, Marin PD, Božić Nedeljković B. Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine.. in 2nd International UNIFood Conference, September 24-25, Belgrade. 2021;:63.
https://hdl.handle.net/21.15107/rcub_rimsi_2176 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Jovanović Marić, Jovana, Vuković-Gačić, Branka, Duletic-Lausevic, Sonja, Marin, Petar D., Božić Nedeljković, Biljana, "Antitumor activity of Lamiaceae plants frequently used in Serbian folk medicine and cuisine." in 2nd International UNIFood Conference, September 24-25, Belgrade (2021):63,
https://hdl.handle.net/21.15107/rcub_rimsi_2176 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Vuković-Gačić, Branka
AU  - Paunović, Momir
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4732
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2175
AB  - Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia
PB  - Aueninstitut Neuburg
C3  - 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
T1  - Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2175
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Vuković-Gačić, Branka and Paunović, Momir",
year = "2021",
abstract = "Surface waters in Serbia are under high anthropogenic pressure. One of the major problems is untreated municipal and industrial wastewaters. Unfortunately, Serbia processes only 5 % of wastewaters before discharging. As a consequence, pollutants such as metals and metalloids from industrial wastewaters, pharmaceuticals, compounds from personal care products, etc. directly endure into surface water. Microbial faecal pollution, as an indicator of presence of human or/and animal pathogens, due to health hazard limits water usage for drinking, recreation, irrigation, etc. In regulations, faecal coliforms, with Escherichia coli as dominant representative, are widely used as faecal indicator bacteria. The aim of this study was to investigate the microbiological al water quality in the Republic of Serbia at the sites which are not routinely investigated within the national monitoring program. In this purpose 78 sites situated on canals, mountain springs, as well as lowland rivers were selected. Defined Substrate Technology was used for determination Most Probable Number (MPN) of E. coli using Colilert-18 System. The water classification system, developed for the Danube River (Kavka et al., 2006) was used. The results indicated that more than 47 % of selected sites are under critical (21.79 %) or strong (25.64 %) faecal pollution. On the other hand, about 32 % (32.05 %) of sites are little polluted and 20.51 % of sites showed moderate pollution. The increasing levels of faecal pollution detected on sites situated downstream of settlements indicated discharge of untreated municipal wastewaters directly into surface water. In further research, the focus will be placed on the determination of the origin of pollution by the employment of microbial source tracking technique. Obtained data will be used for modelling and predicting the effect of detected contamination on the water quality of the major water bodies in the Republic of Serbia",
publisher = "Aueninstitut Neuburg",
journal = "43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin",
title = "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2175"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Đorđević Aleksić, J., Vuković-Gačić, B.,& Paunović, M.. (2021). Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg., 19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Đorđević Aleksić J, Vuković-Gačić B, Paunović M. Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme.. in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin. 2021;:19.
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Vuković-Gačić, Branka, Paunović, Momir, "Mapping of the microbiological water quality of surface waters in Serbia overlooked by the National monitoring programme." in 43rd IAD Conference: Rivers and Floodplains in the Antropocene: Upcoming Challenges in the Danube River Basin (2021):19,
https://hdl.handle.net/21.15107/rcub_rimsi_2175 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2171
AB  - Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.
PB  - Narodna biblioteka Srbije
C3  - 49. konferencija o aktuelnim temama korišćenja i zaštite voda
T1  - Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije
EP  - 40
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2171
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kračun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "Neprerađene komunalne i industrijske otpadne vode su jedan od glavnih zagađivača površinskih voda u Srbiji. Sa komunalim otpadnim vodama u površinske vode dospevaju hemijski polutanti i fekalno zagađenje koje može sadržati za čoveka patogene mikroorganizme. U okviru istraživanja vršena je analiza mikrobiološkog kvaliteta vode sa 40 lokaliteta na teritoriji Republike Srbije koji nisu obuhvaćeni programom rutinskog monitoringa. Prisustvo i broj Escherichia coli je odabrano kao parametar fekalnog zagađenja. Rezultati su pokazali da voda sa 57,5% lokaliteta pripada III i IV klasi. Najveći broj zagađenih lokaliteta se nalazi nizvodno od naseljenih mesta, što potvrđuje značaj izgradnje postrojenja za preradu otpadnih voda.",
publisher = "Narodna biblioteka Srbije",
journal = "49. konferencija o aktuelnim temama korišćenja i zaštite voda",
title = "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije",
pages = "40-37",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2171"
}

Jovanović Marić, J., Kračun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda
Narodna biblioteka Srbije., 37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171

Jovanović Marić J, Kračun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije. in 49. konferencija o aktuelnim temama korišćenja i zaštite voda. 2020;:37-40.
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .

Jovanović Marić, Jovana, Kračun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Analiza mikrobiološkog kvaliteta površinskih voda na teritoriji Republike srbije" in 49. konferencija o aktuelnim temama korišćenja i zaštite voda (2020):37-40,
https://hdl.handle.net/21.15107/rcub_rimsi_2171 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Ilić, Marija
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2161
AB  - Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters.
Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program.
Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR.
Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).
PB  - Federation of European Microbiological Societies - FEMS
C3  - FEMS Online Conference on Microbiology 2020
T1  - Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study
SP  - 403
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2161
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Ilić, Marija and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka",
year = "2020",
abstract = "Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters.
Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program.
Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR.
Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).",
publisher = "Federation of European Microbiological Societies - FEMS",
journal = "FEMS Online Conference on Microbiology 2020",
title = "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study",
pages = "403",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2161"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Ilić, M., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020
Federation of European Microbiological Societies - FEMS., 403.
https://hdl.handle.net/21.15107/rcub_rimsi_2161

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Ilić M, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020. 2020;:403.
https://hdl.handle.net/21.15107/rcub_rimsi_2161 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Ilić, Marija, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study" in FEMS Online Conference on Microbiology 2020 (2020):403,
https://hdl.handle.net/21.15107/rcub_rimsi_2161 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1723
AB  - Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Bioassays in assessment of environmental pollution
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Bioassays in assessment of environmental pollution",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1723"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Bioassays in assessment of environmental pollution" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):115,
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1724
AB  - Untreated municipal wastewaters are one of the major negative contributors on freshwater quality, and consequently on ecosystem balance. Some compounds such as pharmaceuticals, cosmetic products, etc. could be genotoxic and lead to somatic and/or germinative mutations. This mutation should be reflected on organisms’ health and reproductive potential. Comet assay is a widely used test in ecogenotoxicology for detection of primary DNA damage. In that way, this sensitive test could provide information about early warning signs of potential stressors effects before those have an impact on the population or ecosystem level.
The scope of our study was to assess the level of DNA damage of Alburnus alburnus specimens’ gill cells and erythrocytes. Three sites on the Sava river were chosen: reference site Zabran suited upstream the municipal discharging; second at the confluence of the Kolubara river and the Sava river; and the third at the confluence of the Barička river and the Sava river. The Kolubara river and the Barička river are chosen as important recipients of untreated wastewaters. From each site, 5 bleak specimens were collected and blood and gills were taken. Tail Intensity (TI%) was chosen as a parameter for evaluating the level of DNA damage. Besides that, cell viability and extremely damaged cells - hedgehogs (HH) were determined.
The significant difference in TI% values was recorded in erythrocytes and gills cells in comparison between the Zabran (TI%=14.01±0.61 in erythrocytes, TI%=15.25±1.03 in gills cells) and the second site (TI%=18.53±0.65 in erythrocytes, TI%=22.78±1.89 in gills cells). Correlation between cell viability and HH frequency was not observed. According to the results, we could conclude that the second site is the most affected by pollutants. Also, usage of comet assay on freshwater organisms could be appropriate for preliminary screening of ecosystem state.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure
EP  - 119
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1724
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir and Vuković-Gačić, Branka",
year = "2019",
abstract = "Untreated municipal wastewaters are one of the major negative contributors on freshwater quality, and consequently on ecosystem balance. Some compounds such as pharmaceuticals, cosmetic products, etc. could be genotoxic and lead to somatic and/or germinative mutations. This mutation should be reflected on organisms’ health and reproductive potential. Comet assay is a widely used test in ecogenotoxicology for detection of primary DNA damage. In that way, this sensitive test could provide information about early warning signs of potential stressors effects before those have an impact on the population or ecosystem level.
The scope of our study was to assess the level of DNA damage of Alburnus alburnus specimens’ gill cells and erythrocytes. Three sites on the Sava river were chosen: reference site Zabran suited upstream the municipal discharging; second at the confluence of the Kolubara river and the Sava river; and the third at the confluence of the Barička river and the Sava river. The Kolubara river and the Barička river are chosen as important recipients of untreated wastewaters. From each site, 5 bleak specimens were collected and blood and gills were taken. Tail Intensity (TI%) was chosen as a parameter for evaluating the level of DNA damage. Besides that, cell viability and extremely damaged cells - hedgehogs (HH) were determined.
The significant difference in TI% values was recorded in erythrocytes and gills cells in comparison between the Zabran (TI%=14.01±0.61 in erythrocytes, TI%=15.25±1.03 in gills cells) and the second site (TI%=18.53±0.65 in erythrocytes, TI%=22.78±1.89 in gills cells). Correlation between cell viability and HH frequency was not observed. According to the results, we could conclude that the second site is the most affected by pollutants. Also, usage of comet assay on freshwater organisms could be appropriate for preliminary screening of ecosystem state.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure",
pages = "119-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1724"
}

Jovanović Marić, J., Đorđević Aleksić, J., Kolarević, S., Kračun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Paunović, M.,& Vuković-Gačić, B.. (2019). Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 118-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1724

Jovanović Marić J, Đorđević Aleksić J, Kolarević S, Kračun-Kolarević M, Kostić-Vuković J, Sunjog K, Paunović M, Vuković-Gačić B. Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:118-119.
https://hdl.handle.net/21.15107/rcub_rimsi_1724 .

Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, Vuković-Gačić, Branka, "Comet assay – a sensitive method for detection DNA damage and primary monitoring of ecosystem pollution pressure" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):118-119,
https://hdl.handle.net/21.15107/rcub_rimsi_1724 .

TY  - CONF
AU  - Kolarević, Stoimir
AU  - Kostić-Vuković, Jovana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Gacic, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1922
AB  - Narušavanje kvaliteta površinskih vodotokova otpadnim vodama se odražava na kvalitet akvatičnih ekosistema ali i na kvalitet života i zdravlja ljudi. Variranje u stepenu prerade otpadnih voda najčešće je posledica razlika u nacionalnim zakonskim regulativama što je veoma evidentno u slučaju reke Dunav, u nekim od podunavskih zemalja voda se prerađuje dok se u drugim nikakav tretman ne primenjuje pre ispuštanja. JDS3 (The Joint Danube Survey 3) je bila najveća rečna ekspedicija u 2013. godini koja je obuhvatila svih X sektora reke. Zbog obima uzoraka i različitih analiza, JDS je predstavljao idealnu priliku da se ispita prisustvo genotoksičnog zagađenja u ovoj reci. Komet test je rađen na hemocitama školjki Unio sp. i Sinanodanta woodiana. Najviši nivo genotoksičnog potencijala detektovan je u sektoru VI (Panonska ravan) gde veliki problem predstavljaju otpadne vode koje se bez prerade ispuštaju u Dunav. Ova studija je dala mapu zagađenja i kritičnih tačaka zagađenja duž reke Dunav i ukazala na bitnost prerade otpadnih voda za očuvanje i poboljšanje kvaliteta reke Dunav. Uvođenje molekularnih markera u monitoring kvaliteta voda znatno doprinosi razumevanju prirode i porekla zagađenja.
PB  - Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, Republika Srbija
T1  - Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3
SP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1922
ER  - 

@conference{
author = "Kolarević, Stoimir and Kostić-Vuković, Jovana and Kračun-Kolarević, Margareta and Paunović, Momir and Gacic, Zoran and Vuković-Gačić, Branka",
year = "2018",
abstract = "Narušavanje kvaliteta površinskih vodotokova otpadnim vodama se odražava na kvalitet akvatičnih ekosistema ali i na kvalitet života i zdravlja ljudi. Variranje u stepenu prerade otpadnih voda najčešće je posledica razlika u nacionalnim zakonskim regulativama što je veoma evidentno u slučaju reke Dunav, u nekim od podunavskih zemalja voda se prerađuje dok se u drugim nikakav tretman ne primenjuje pre ispuštanja. JDS3 (The Joint Danube Survey 3) je bila najveća rečna ekspedicija u 2013. godini koja je obuhvatila svih X sektora reke. Zbog obima uzoraka i različitih analiza, JDS je predstavljao idealnu priliku da se ispita prisustvo genotoksičnog zagađenja u ovoj reci. Komet test je rađen na hemocitama školjki Unio sp. i Sinanodanta woodiana. Najviši nivo genotoksičnog potencijala detektovan je u sektoru VI (Panonska ravan) gde veliki problem predstavljaju otpadne vode koje se bez prerade ispuštaju u Dunav. Ova studija je dala mapu zagađenja i kritičnih tačaka zagađenja duž reke Dunav i ukazala na bitnost prerade otpadnih voda za očuvanje i poboljšanje kvaliteta reke Dunav. Uvođenje molekularnih markera u monitoring kvaliteta voda znatno doprinosi razumevanju prirode i porekla zagađenja.",
publisher = "Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, Republika Srbija",
title = "Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3",
pages = "85",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1922"
}

Kolarević, S., Kostić-Vuković, J., Kračun-Kolarević, M., Paunović, M., Gacic, Z.,& Vuković-Gačić, B.. (2018). Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija
Srpsko biološko društvo., 85.
https://hdl.handle.net/21.15107/rcub_rimsi_1922

Kolarević S, Kostić-Vuković J, Kračun-Kolarević M, Paunović M, Gacic Z, Vuković-Gačić B. Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija. 2018;:85.
https://hdl.handle.net/21.15107/rcub_rimsi_1922 .

Kolarević, Stoimir, Kostić-Vuković, Jovana, Kračun-Kolarević, Margareta, Paunović, Momir, Gacic, Zoran, Vuković-Gačić, Branka, "Uticaj otpadnih voda na reku Dunav – ekogenotoksikološki aspekt ekspedicije JDS3" in Drugi kongres biologa Srbije, Kladovo, Republika Srbija (2018):85,
https://hdl.handle.net/21.15107/rcub_rimsi_1922 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1667
AB  - Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.
PB  - Institute of Geography of SAS
C3  - The 42nd IAD Conference 2018, Smolenice, Slovakia
T1  - DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1667
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.",
publisher = "Institute of Geography of SAS",
journal = "The 42nd IAD Conference 2018, Smolenice, Slovakia",
title = "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1667"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia
Institute of Geography of SAS., 34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia. 2018;:34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution" in The 42nd IAD Conference 2018, Smolenice, Slovakia (2018):34-34,
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1681
AB  - The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)
EP  - 162
SP  - 162
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1681
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)",
pages = "162-162",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1681"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):162-162,
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Paunović, Momir
AU  - Simonovic, Predrag
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1913
AB  - One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1913
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Đorđević Aleksić, Jelena and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir and Simonovic, Predrag and Vuković-Gačić, Branka",
year = "2018",
abstract = "One of the major limitations in performing ecogenotoxicological studies is the distance between research field and the laboratory. As some of the methods used in ecogenotoxicology require fresh biological material with intact cell viability, transfer of samples to the laboratory within a few hours after sampling is usually required. To overcome this issue, we have introduced cryopreservation in our research as a possible solution. Cryopreservation is a method which includes preservation of intact, living cells at low temperature for a long time. In natural conditions freezing, forming of ice crystals and dehydration could destroy cell structures. To avoid this consequence, specific compounds were introduced, cryoprotective agents, in the method of cryopreservation. The main characteristic of these compounds is their ability to reduce ice crystal formation in cells at any temperature.
We have applied cryopreservation in the evaluation of genotoxic potential along different river streams (the Adige River, the Sava River and the Velika Morava River basin). For this purpose, we focused on the level of DNA damage of cryopreserved fish blood cells (Salmo cenerinus, Salmo marmoratus, Alburnus alburnus) by using the comet assay.
To test whether cryopreservation has the impact on cell viability, or that it induces additional DNA damage, we employed preliminary experiments in 4 Abramis brama and 8 A. alburnus specimens. Namely, from every specimen two blood samples were taken: one for analyzing cells viability and the level of DNA damage of fresh blood, and another for observing cell viability and DNA damage of cryopreserved samples. The viability of cell blood was determined by using acridine orange/ethidium bromide differential staining. For analyzing the level of DNA damage alkaline comet assay was used. Obtained results indicated that cryopreserved blood cells had approximately the same viability and the level of DNA damage as nonpreserved blood samples.
According to our results, cryopreservation is a very useful method in genotoxicology and could have many benefits: blood samples should not be analyzed immediately after sampling; samples could be transported in liquid nitrogen without concern about additional DNA damage.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems",
pages = "134",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1913"
}

Jovanović Marić, J., Đorđević Aleksić, J., Kolarević, S., Kračun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Paunović, M., Simonovic, P.,& Vuković-Gačić, B.. (2018). Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913

Jovanović Marić J, Đorđević Aleksić J, Kolarević S, Kračun-Kolarević M, Kostić-Vuković J, Sunjog K, Paunović M, Simonovic P, Vuković-Gačić B. Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:134.
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .

Jovanović Marić, Jovana, Đorđević Aleksić, Jelena, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, Simonovic, Predrag, Vuković-Gačić, Branka, "Cryopreservation of fish blood – useful tool for assessing genotoxic potential of aquatic ecosystems" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):134,
https://hdl.handle.net/21.15107/rcub_rimsi_1913 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Tenji, Dina
AU  - Teodorović, Ivana
AU  - Sipos, Sandor
AU  - Paunović, Momir
AU  - Deutschmann, Bjorn
AU  - Seiler, Thomas-Benjamin
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1728
AB  - Ispuštanje otpadnih voda u površinske vode predstavlja globalni problem jer negativno utiče na kvalitet voda, vodene organizme i zdravlje ljudi. U Srbiji se prerađuje svega 5–10% otpadnih voda.1 U ovoj studiji praćen je uticaj otpadnih voda na nivo DNK oštećenja školjki (Sinanodonta woodiana L.) i riba (Cyprinus carpio L.). Oštećenja DNK u ćelijama hemolimfe i krvi praćeno je alkalnim komet testom (Single Cell Gel Electroforesis, SCGE), koji detektuje biomarkere izlaganja i mikronukleus testom, kojim se prate biomarkeri efekta. Kavezi sa organizmima koji su aklimatizovani u laboratorijskim uslovima su stavljeni na tri lokaliteta na Dunavu: uzvodno od Novog Sada, neposredno nizvodno od ispusta gradske kanalizacije i 7 km nizvodno od Novog Sada. Sa svakog lokaliteta je analizirano 5 jedinki školjki i 10 jedinki riba. a kontrolu su izabrane aklimatizovane jedinke koje nisu izlagane na lokalitetima. Rezultati komet testa pokazali su da je najviši nivo DNK oštećenja u odnosu na kontrolu uočen kod jedinki na mestu ispusta kanalizacije, što je u skladu sa dobijenim hemijskim analizama na ispitivanim lokalitetima.2,3 Za razliku od komet testa, rezultati mikronukleus testa nisu pokazali statistički značajnu razliku između kontrole i odabranih lokaliteta. Dobijeni rezultati ukazuju da su odabrani testovi pokazali različitu oseljivost, jer detektuju različite tipove biomarkera. Međutim, oba testa su ukazala na najviši nivo DNK oštećenja na lokalitetu koji je najizloženiji polutantima.
PB  - Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018
T1  - Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1728
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Tenji, Dina and Teodorović, Ivana and Sipos, Sandor and Paunović, Momir and Deutschmann, Bjorn and Seiler, Thomas-Benjamin and Vuković-Gačić, Branka",
year = "2018",
abstract = "Ispuštanje otpadnih voda u površinske vode predstavlja globalni problem jer negativno utiče na kvalitet voda, vodene organizme i zdravlje ljudi. U Srbiji se prerađuje svega 5–10% otpadnih voda.1 U ovoj studiji praćen je uticaj otpadnih voda na nivo DNK oštećenja školjki (Sinanodonta woodiana L.) i riba (Cyprinus carpio L.). Oštećenja DNK u ćelijama hemolimfe i krvi praćeno je alkalnim komet testom (Single Cell Gel Electroforesis, SCGE), koji detektuje biomarkere izlaganja i mikronukleus testom, kojim se prate biomarkeri efekta. Kavezi sa organizmima koji su aklimatizovani u laboratorijskim uslovima su stavljeni na tri lokaliteta na Dunavu: uzvodno od Novog Sada, neposredno nizvodno od ispusta gradske kanalizacije i 7 km nizvodno od Novog Sada. Sa svakog lokaliteta je analizirano 5 jedinki školjki i 10 jedinki riba. a kontrolu su izabrane aklimatizovane jedinke koje nisu izlagane na lokalitetima. Rezultati komet testa pokazali su da je najviši nivo DNK oštećenja u odnosu na kontrolu uočen kod jedinki na mestu ispusta kanalizacije, što je u skladu sa dobijenim hemijskim analizama na ispitivanim lokalitetima.2,3 Za razliku od komet testa, rezultati mikronukleus testa nisu pokazali statistički značajnu razliku između kontrole i odabranih lokaliteta. Dobijeni rezultati ukazuju da su odabrani testovi pokazali različitu oseljivost, jer detektuju različite tipove biomarkera. Međutim, oba testa su ukazala na najviši nivo DNK oštećenja na lokalitetu koji je najizloženiji polutantima.",
publisher = "Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018",
title = "Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1728"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Tenji, D., Teodorović, I., Sipos, S., Paunović, M., Deutschmann, B., Seiler, T.,& Vuković-Gačić, B.. (2018). Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018
Srpsko biološko društvo., 110.
https://hdl.handle.net/21.15107/rcub_rimsi_1728

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Sunjog K, Kostić-Vuković J, Tenji D, Teodorović I, Sipos S, Paunović M, Deutschmann B, Seiler T, Vuković-Gačić B. Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018. 2018;:110.
https://hdl.handle.net/21.15107/rcub_rimsi_1728 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Tenji, Dina, Teodorović, Ivana, Sipos, Sandor, Paunović, Momir, Deutschmann, Bjorn, Seiler, Thomas-Benjamin, Vuković-Gačić, Branka, "Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija" in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018 (2018):110,
https://hdl.handle.net/21.15107/rcub_rimsi_1728 .

TY  - CONF
AU  - Kračun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1720
AB  - Seeking for a better quality of life, consumption of pharmaceuticals is constantly increasing. Pharmaceuticals are designed to be more potent, degradation resistant, to affect protein targets at relatively low doses. Many of them are not selective and became threat to non-target organisms, especially to one living in aquatic ecosystems. Because of the inability of wastewater treatment plants to eliminate these compounds in the end they reach aquatic environments through sewage system.
Aquatic invertebrates spend at least a part of their life cycle in the aquatic environment. Mobility is not one of the traits that characterize many species of aquatic invertebrates, especially freshwater mussels and aquatic worms which are almost sedentary organisms. Due to their way of life, this species are under the influence of variety of pollutants via sediment and via water column.
Genotoxic effects of cytostatics with different mode of action – alkylation agent (cisplatin - CP), antimetabolite agent (5-fluorouracil – 5-FU), plant alkaloids (etoposide - ETO, vincristine sulphate - VIN) and other neoplastic agent (imatinib mesylate - IM) were studied in vivo and in vitro on haemocytes of two freshwater mussels species Unio sp. (U. pictorum/U. tumidus), and in vivo on haemocytes and coelomocytes of tubificid species Limnodrilus udekemianus.
Experiments were organized as short-term treatments (72h for mussels/96h for worms) in static system. Level of DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay). Based on our results ranking of cytostatics by their effects on mussels was VIN>5-FU>ETO>CP>IM and on worms was 5-FU>ET. Worms have shown higher sensitivity for the negative effects of 5-FU and ET on the integrity of DNA molecule comparing with mussels. The lowest observed effect concentration (LOEC) for 5-FU was 52 μg/L for mussels and 0.52μg/L for worms. In the case of ET LOEC was 24 mg/L for mussels and 0.024 mg/L for worms. For VIN was detected difference in the response in U. pictorum and U. tumidus – LOEC for U. pictorum was 3.7 μg/L, while for U. tumidus was 36.9 μg/L. Significant damage of DNA wasn’t detected for CP and IM. Although the PEC values for tested cytostatics are lower than the ones used in our study, it must be emphasized that in the environment, organisms are under constant influence of these pollutants and organisms are struggling with the effects of mixture of pharmaceuticals and mixture of different pollutants. Impacts of these mixtures on the aquatic organisms are still unknown, and therefore, further research should consider this fact and the studies should be organized in this direction.
Acknowledgements: EU Seventh Framework Programme – Cytothreat (no. 265264). Ministry of Education, Science and Technological Development of the Republic of Serbia project no. 037009.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?
SP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1720
ER  - 

@conference{
author = "Kračun-Kolarević, Margareta and Kolarević, Stoimir and Kostić-Vuković, Jovana and Sunjog, Karolina and Gačić, Zoran and Paunović, Momir and Vuković-Gačić, Branka",
year = "2018",
abstract = "Seeking for a better quality of life, consumption of pharmaceuticals is constantly increasing. Pharmaceuticals are designed to be more potent, degradation resistant, to affect protein targets at relatively low doses. Many of them are not selective and became threat to non-target organisms, especially to one living in aquatic ecosystems. Because of the inability of wastewater treatment plants to eliminate these compounds in the end they reach aquatic environments through sewage system.
Aquatic invertebrates spend at least a part of their life cycle in the aquatic environment. Mobility is not one of the traits that characterize many species of aquatic invertebrates, especially freshwater mussels and aquatic worms which are almost sedentary organisms. Due to their way of life, this species are under the influence of variety of pollutants via sediment and via water column.
Genotoxic effects of cytostatics with different mode of action – alkylation agent (cisplatin - CP), antimetabolite agent (5-fluorouracil – 5-FU), plant alkaloids (etoposide - ETO, vincristine sulphate - VIN) and other neoplastic agent (imatinib mesylate - IM) were studied in vivo and in vitro on haemocytes of two freshwater mussels species Unio sp. (U. pictorum/U. tumidus), and in vivo on haemocytes and coelomocytes of tubificid species Limnodrilus udekemianus.
Experiments were organized as short-term treatments (72h for mussels/96h for worms) in static system. Level of DNA damage was evaluated by alkaline single-cell gel electrophoresis (comet assay). Based on our results ranking of cytostatics by their effects on mussels was VIN>5-FU>ETO>CP>IM and on worms was 5-FU>ET. Worms have shown higher sensitivity for the negative effects of 5-FU and ET on the integrity of DNA molecule comparing with mussels. The lowest observed effect concentration (LOEC) for 5-FU was 52 μg/L for mussels and 0.52μg/L for worms. In the case of ET LOEC was 24 mg/L for mussels and 0.024 mg/L for worms. For VIN was detected difference in the response in U. pictorum and U. tumidus – LOEC for U. pictorum was 3.7 μg/L, while for U. tumidus was 36.9 μg/L. Significant damage of DNA wasn’t detected for CP and IM. Although the PEC values for tested cytostatics are lower than the ones used in our study, it must be emphasized that in the environment, organisms are under constant influence of these pollutants and organisms are struggling with the effects of mixture of pharmaceuticals and mixture of different pollutants. Impacts of these mixtures on the aquatic organisms are still unknown, and therefore, further research should consider this fact and the studies should be organized in this direction.
Acknowledgements: EU Seventh Framework Programme – Cytothreat (no. 265264). Ministry of Education, Science and Technological Development of the Republic of Serbia project no. 037009.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1720"
}

Kračun-Kolarević, M., Kolarević, S., Kostić-Vuković, J., Sunjog, K., Gačić, Z., Paunović, M.,& Vuković-Gačić, B.. (2018). Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 135.
https://hdl.handle.net/21.15107/rcub_rimsi_1720

Kračun-Kolarević M, Kolarević S, Kostić-Vuković J, Sunjog K, Gačić Z, Paunović M, Vuković-Gačić B. Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?. in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:135.
https://hdl.handle.net/21.15107/rcub_rimsi_1720 .

Kračun-Kolarević, Margareta, Kolarević, Stoimir, Kostić-Vuković, Jovana, Sunjog, Karolina, Gačić, Zoran, Paunović, Momir, Vuković-Gačić, Branka, "Cytostatics as emerging pollutants – is there a threat for aquatic invertebrates?" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):135,
https://hdl.handle.net/21.15107/rcub_rimsi_1720 .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Milošković, Aleksandra
AU  - Radojković, Nataša
AU  - Simić, Vera
AU  - Dojčinović, Biljana
AU  - Kračun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijić, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2150
AB  - Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.
PB  - Balkan Society for Microbiology
C3  - 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
T1  - Application of sos/umuc assay in eco/genotoxicology
EP  - 385
SP  - 385
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2150
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Radojković, Nataša and Simić, Vera and Dojčinović, Biljana and Kračun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijić, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Vuković-Gačić, Branka",
year = "2017",
abstract = "Abstract:
Introduction. The SOS/umuC assay is used for assessment of water genotoxicity. Salmonella typhimurium TA1535/pSK1002 is used in this test, but the results can be extrapolated on higher eukaryotic organisms with the introduction of enzymatic S9 fraction in experimental procedure. The test is standardised for the determination of the genotoxic potential of water and wastewater (ISO/DIS 13829, 2000). Aim. Sensitivity of the assay was challenged by using parallel in situ and in vitro approach in evaluation of the genotoxic potential in the basins of significant tributaries of the Danube River: the Sava River and the Velika Morava River. Materials and methods. Within in vitro testing, native water samples were analysed by SOS/umuC test. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet and micronucleus assays. The concentration of heavy metals in fish tissue and the data of the physico-chemical parameters measured in water were used as a measure of the pollution pressure at the sites. Results. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests. None of 20 investigated samples showed genotoxic potential in SOS/umuC assay while in situ analyses indicated variation of genotoxic potential among the investigated sites. Conclusions. The results of our study point towards low sensitivity of the SOS/umuC test when processing un-concentrated (native) water samples; the results of in vitro tests should be taken with precaution when making predictions on the status of the ecosystem.",
publisher = "Balkan Society for Microbiology",
journal = "10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017",
title = "Application of sos/umuc assay in eco/genotoxicology",
pages = "385-385",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2150"
}

Jovanović Marić, J., Kolarević, S., Milošković, A., Radojković, N., Simić, V., Dojčinović, B., Kračun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijić, J., Đorđević Aleksić, J., Gačić, Z.,& Vuković-Gačić, B.. (2017). Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017
Balkan Society for Microbiology., 385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150

Jovanović Marić J, Kolarević S, Milošković A, Radojković N, Simić V, Dojčinović B, Kračun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijić J, Đorđević Aleksić J, Gačić Z, Vuković-Gačić B. Application of sos/umuc assay in eco/genotoxicology. in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017. 2017;:385-385.
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Radojković, Nataša, Simić, Vera, Dojčinović, Biljana, Kračun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijić, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Vuković-Gačić, Branka, "Application of sos/umuc assay in eco/genotoxicology" in 10th Balkan Congress of Microbiology: Microbiologia Balkanica 2017 (2017):385-385,
https://hdl.handle.net/21.15107/rcub_rimsi_2150 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gacic, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1927
AB  - Due to the presence of a large number of different pollutants, monitoring of the surface water quality based solely on the analysis of a limited number of xenobiotics, cannot be considered as reliable. Beside toxic, these agents can exert genotoxic effects, inducing damage in the DNA molecule, which, if not repaired, could lead to mutations and alterations in cells, tissues, organism, whole population and the ecosystem. The surface waters are under the pressure of both anthropogenic and natural sources of pollution. Additionally, extreme hydrological events, such as water scarcity and flooding, may further impair the state of freshwater bodies. Fish may be exposed to harmful substances through water, sediment and food. In ecogenotoxicity studies, gills are used as they represent the first organ in direct contact with water and waterborne pollutants, while the liver, as a key organ that controls many life functions is used as a major organ for metabolic breakdown of xenobiotics. Common bream (Abramis brama), white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa) are three closely related, benthivorous cyprinids, native for the Sava River. The sampling site Duboko (23 rkm), on the Sava River, is chosen as it is exposed to the untreated wastewater from the town of Obrenovac (more than 70,000 inhabitants), intensive agricultural activity and close proximity to the largest thermal power plant in Serbia (TENTA) and belonging ash field. This study was conducted to assess the impact of multiple stressors during different seasons on different levels of biological organization, subcellular (genotoxic effect) and cellular/tissue level (histopathological effects), in the liver and gills of three bream species. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. In parallel, concentration of metals and metalloids were assessed in gills, liver and muscle.
Basic physical (pH, temperature, oxygen concentration, electrical conductivity) and chemical (NO2, NO3-, NH4+, PO43-) parameters were measured on site. Microbiological indicators of faecal pollution, total coliforms (TC), Escherichia coli (EC) and Enterococci (EF) were assessed by using a most probable number approach (MPN). Presumptive Clostridium perfringens (CP) numbers were determined by using membrane filtration and incubation on TSC (Tryptose Sulphite Cycloserine) media. Sampling of fish tissue for comet assay and histopathological analyses was performed in 2014, during winter (January and February), spring (March and early June), and summer (late June, July, and August), once per month, for a total of 52 specimens. Analysis of metals and metalloids was performed only on fish sampled in February, early June and in August, in total 15 specimens. In the mid May extreme hydrological events led to extensive flooding in the studied area. Comet assay was conducted on the liver and gill cells according to Kostić et al. Tail intensity, % of DNA in the comet tail (TI) was used to express the DNA damage level. Histopathological analyses included the use of conventional histological methods, staining of tissues sections using hematoxylin eosine differential staining, and examined under the microscope. The type and the extent of histological alterations were described by using a method developed by Bernet et al. Analysis of metals and metalloids was performed by inductively-coupled plasma optical emission spectrometry (ICP-OES), and included assessment of concentrations of 16 elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr and Zn). To compare the total metal content in different tissues and through different seasons metal pollution index (MPI) was calculated according to equation MPI = (cf1 x cf2 x cf3 x…cfn) 1/n, where cfn = concentration of the metal n in the sample.
Statistical analysis of data from the individual months showed the highest DNA damage in gill cells during early June (spring). Gill histopathological index (IG) did not show significant seasonal variations, however it was the lowest during winter, the highest in spring, and slightly decreased in summer. A possible cause of this incidence could be a withdrawal of water which took place in June, after the flooding event that occurred in the middle of May. In liver the highest DNA damage was observed during August. Histopathological index of liver showed significantly higher values in summer in comparison to spring. This could be prescribed to a higher metabolic rate of fish liver during warm seasons and also could be a consequence of processing a large quantities of xenobiotics introduced into the water column due to withdrawal of water after floods and sediment disturbance. According to the MPI, gills were under the highest pressure of metal pollution during spring and summer. Liver was under the highest pressure of metal pollution during winter, while the muscle was the least affected tissue during all three seasons.
Overall, gills as the first organ in direct contact with water showed a higher response in terms of DNA damage (molecular level), while the liver as the major organ for processing of xenobiotics both from water and food showed a higher degree of histopathological alterations in comparison to gills (tissue/organ level). Increased response of both biomarkers during spring and summer indicates a joint effect of the flooding event and seasonal changes of climate and hydrological parameters.
PB  - Institute for Water Quality, Resource and Waste Management TU Wien
C3  - International Conference River Basins 2017, Vienna, Austria
T1  - The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1927
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2017",
abstract = "Due to the presence of a large number of different pollutants, monitoring of the surface water quality based solely on the analysis of a limited number of xenobiotics, cannot be considered as reliable. Beside toxic, these agents can exert genotoxic effects, inducing damage in the DNA molecule, which, if not repaired, could lead to mutations and alterations in cells, tissues, organism, whole population and the ecosystem. The surface waters are under the pressure of both anthropogenic and natural sources of pollution. Additionally, extreme hydrological events, such as water scarcity and flooding, may further impair the state of freshwater bodies. Fish may be exposed to harmful substances through water, sediment and food. In ecogenotoxicity studies, gills are used as they represent the first organ in direct contact with water and waterborne pollutants, while the liver, as a key organ that controls many life functions is used as a major organ for metabolic breakdown of xenobiotics. Common bream (Abramis brama), white bream (Blicca bjoerkna) and white-eye bream (Ballerus sapa) are three closely related, benthivorous cyprinids, native for the Sava River. The sampling site Duboko (23 rkm), on the Sava River, is chosen as it is exposed to the untreated wastewater from the town of Obrenovac (more than 70,000 inhabitants), intensive agricultural activity and close proximity to the largest thermal power plant in Serbia (TENTA) and belonging ash field. This study was conducted to assess the impact of multiple stressors during different seasons on different levels of biological organization, subcellular (genotoxic effect) and cellular/tissue level (histopathological effects), in the liver and gills of three bream species. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. In parallel, concentration of metals and metalloids were assessed in gills, liver and muscle.
Basic physical (pH, temperature, oxygen concentration, electrical conductivity) and chemical (NO2, NO3-, NH4+, PO43-) parameters were measured on site. Microbiological indicators of faecal pollution, total coliforms (TC), Escherichia coli (EC) and Enterococci (EF) were assessed by using a most probable number approach (MPN). Presumptive Clostridium perfringens (CP) numbers were determined by using membrane filtration and incubation on TSC (Tryptose Sulphite Cycloserine) media. Sampling of fish tissue for comet assay and histopathological analyses was performed in 2014, during winter (January and February), spring (March and early June), and summer (late June, July, and August), once per month, for a total of 52 specimens. Analysis of metals and metalloids was performed only on fish sampled in February, early June and in August, in total 15 specimens. In the mid May extreme hydrological events led to extensive flooding in the studied area. Comet assay was conducted on the liver and gill cells according to Kostić et al. Tail intensity, % of DNA in the comet tail (TI) was used to express the DNA damage level. Histopathological analyses included the use of conventional histological methods, staining of tissues sections using hematoxylin eosine differential staining, and examined under the microscope. The type and the extent of histological alterations were described by using a method developed by Bernet et al. Analysis of metals and metalloids was performed by inductively-coupled plasma optical emission spectrometry (ICP-OES), and included assessment of concentrations of 16 elements (Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Li, Mn, Mo, Ni, Pb, Sr and Zn). To compare the total metal content in different tissues and through different seasons metal pollution index (MPI) was calculated according to equation MPI = (cf1 x cf2 x cf3 x…cfn) 1/n, where cfn = concentration of the metal n in the sample.
Statistical analysis of data from the individual months showed the highest DNA damage in gill cells during early June (spring). Gill histopathological index (IG) did not show significant seasonal variations, however it was the lowest during winter, the highest in spring, and slightly decreased in summer. A possible cause of this incidence could be a withdrawal of water which took place in June, after the flooding event that occurred in the middle of May. In liver the highest DNA damage was observed during August. Histopathological index of liver showed significantly higher values in summer in comparison to spring. This could be prescribed to a higher metabolic rate of fish liver during warm seasons and also could be a consequence of processing a large quantities of xenobiotics introduced into the water column due to withdrawal of water after floods and sediment disturbance. According to the MPI, gills were under the highest pressure of metal pollution during spring and summer. Liver was under the highest pressure of metal pollution during winter, while the muscle was the least affected tissue during all three seasons.
Overall, gills as the first organ in direct contact with water showed a higher response in terms of DNA damage (molecular level), while the liver as the major organ for processing of xenobiotics both from water and food showed a higher degree of histopathological alterations in comparison to gills (tissue/organ level). Increased response of both biomarkers during spring and summer indicates a joint effect of the flooding event and seasonal changes of climate and hydrological parameters.",
publisher = "Institute for Water Quality, Resource and Waste Management TU Wien",
journal = "International Conference River Basins 2017, Vienna, Austria",
title = "The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1927"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Aborgiba, M., Gacic, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2017). The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species. in International Conference River Basins 2017, Vienna, Austria
Institute for Water Quality, Resource and Waste Management TU Wien., 8.
https://hdl.handle.net/21.15107/rcub_rimsi_1927

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Aborgiba M, Gacic Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species. in International Conference River Basins 2017, Vienna, Austria. 2017;:8.
https://hdl.handle.net/21.15107/rcub_rimsi_1927 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "The impact of the Sava river pollution on biomarkers response in the liver and gills of three cyprinid species" in International Conference River Basins 2017, Vienna, Austria (2017):8,
https://hdl.handle.net/21.15107/rcub_rimsi_1927 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Knežević-Vukčević, Jelena
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1926
AB  - U ovom radu ispitivan je potencijal dve vrste roda Abramis (Abramis bjoerkna i Abramis
sapa) kao bioindikatora genotoksičnog potencijala vode reke Save primenom komet
testa. Izabrani lokalitet pripada oblasti intezivne poljoprivredne aktivnosti, izložen je otpadnim
vodama grada Obrenovca i nalazi se u blizini najveće termoelektrane u Srbiji, “Nikola
Tesla”. Uzorkovanje je vršeno od januara do avgusta 2014. godine, obuhvatajući mesec
maj kada je došlo do intezivnih poplava u slivu reke Save. Dobijeni rezultati ukazuju da se
ispitivane vrste mogu koristiti za procenu genotoksičnog potencijala.
PB  - University of Belgrade, Faculty of Agriculture
C3  - VII International Conference Water & Fish, Belgrade, Serbia
T1  - How flooding modifies genotoxic response in freshwater fish?
SP  - 333
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1926
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Aborgiba, Mustafa and Knežević-Vukčević, Jelena and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2015",
abstract = "U ovom radu ispitivan je potencijal dve vrste roda Abramis (Abramis bjoerkna i Abramis
sapa) kao bioindikatora genotoksičnog potencijala vode reke Save primenom komet
testa. Izabrani lokalitet pripada oblasti intezivne poljoprivredne aktivnosti, izložen je otpadnim
vodama grada Obrenovca i nalazi se u blizini najveće termoelektrane u Srbiji, “Nikola
Tesla”. Uzorkovanje je vršeno od januara do avgusta 2014. godine, obuhvatajući mesec
maj kada je došlo do intezivnih poplava u slivu reke Save. Dobijeni rezultati ukazuju da se
ispitivane vrste mogu koristiti za procenu genotoksičnog potencijala.",
publisher = "University of Belgrade, Faculty of Agriculture",
journal = "VII International Conference Water & Fish, Belgrade, Serbia",
title = "How flooding modifies genotoxic response in freshwater fish?",
pages = "333",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1926"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Aborgiba, M., Knežević-Vukčević, J., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2015). How flooding modifies genotoxic response in freshwater fish?. in VII International Conference Water & Fish, Belgrade, Serbia
University of Belgrade, Faculty of Agriculture., 333.
https://hdl.handle.net/21.15107/rcub_rimsi_1926

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Aborgiba M, Knežević-Vukčević J, Gačić Z, Lenhardt M, Vuković-Gačić B. How flooding modifies genotoxic response in freshwater fish?. in VII International Conference Water & Fish, Belgrade, Serbia. 2015;:333.
https://hdl.handle.net/21.15107/rcub_rimsi_1926 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Aborgiba, Mustafa, Knežević-Vukčević, Jelena, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "How flooding modifies genotoxic response in freshwater fish?" in VII International Conference Water & Fish, Belgrade, Serbia (2015):333,
https://hdl.handle.net/21.15107/rcub_rimsi_1926 .