Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study
Аутори
Jovanović Marić, JovanaKolarević, Stoimir
Kračun-Kolarević, Margareta
Ilić, Marija
Đorđević Aleksić, Jelena
Paunović, Momir
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
,
Federation of European Microbiological Societies - FEMS
Метаподаци
Приказ свих података о документуАпстракт
Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters.
Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program.
Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum ...and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR.
Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).
Кључне речи:
river pollution / Montenegro / water quality / Colilert / wastewaterИзвор:
FEMS Online Conference on Microbiology 2020, 2020, 403-Издавач:
- Federation of European Microbiological Societies - FEMS
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Jovanović Marić, Jovana AU - Kolarević, Stoimir AU - Kračun-Kolarević, Margareta AU - Ilić, Marija AU - Đorđević Aleksić, Jelena AU - Paunović, Momir AU - Vuković-Gačić, Branka PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2161 AB - Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters. Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program. Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR. Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River). PB - Federation of European Microbiological Societies - FEMS C3 - FEMS Online Conference on Microbiology 2020 T1 - Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study SP - 403 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2161 ER -
@conference{ author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Ilić, Marija and Đorđević Aleksić, Jelena and Paunović, Momir and Vuković-Gačić, Branka", year = "2020", abstract = "Background: Montenegro is characterized by numerous mountain springs and larger streams. Many of them are used as sources of drinking water, irrigation, but also for tourists’ recreation: kayaking, rafting, etc. Unfortunately, there is a lack of wastewater treatment plants as well as information about microbial water quality of many surface and ground waters. Objectives: Taking into consideration issue of scarce data about microbial water quality, the aim of this study was to locate hotspots of faecal pollution and investigate the source of pollution. Within the study, 25 sites were selected while three of them were not included in National monitoring program. Methods: To quantify the most probable number of Escherichia coli we used Colilert-18 System. The water quality based on this parameter was assessed according to Kirshner et al. (2009). For determination of the pollution origin, microbial source tracking method was employed. Indicators of human faecal pollution, markers BacHum and HF183II, and animal faecal pollution, markers BacR and Pig2Bac, were quantified with qPCR. Results: According to most probable number of E. coli, 40% sites were little polluted, 36% moderately, 16% critically and per 4% heavily and excessively polluted. Analysis of genetic faecal markers indicated the prevalence of human-as- sociated faecal pollution at all sites. Considering the results of the study, four sites were classified as hotspots of faecal pollution: downstream Pljevlja (Ćehotina River), downstream Vezišnica (Ćehotina River), wastewater outlet at Podgori- ca (Morača River), and downstream Bijelo Polje (Lim River).", publisher = "Federation of European Microbiological Societies - FEMS", journal = "FEMS Online Conference on Microbiology 2020", title = "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study", pages = "403", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2161" }
Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Ilić, M., Đorđević Aleksić, J., Paunović, M.,& Vuković-Gačić, B.. (2020). Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020 Federation of European Microbiological Societies - FEMS., 403. https://hdl.handle.net/21.15107/rcub_rimsi_2161
Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Ilić M, Đorđević Aleksić J, Paunović M, Vuković-Gačić B. Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study. in FEMS Online Conference on Microbiology 2020. 2020;:403. https://hdl.handle.net/21.15107/rcub_rimsi_2161 .
Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Ilić, Marija, Đorđević Aleksić, Jelena, Paunović, Momir, Vuković-Gačić, Branka, "Microbial quality and pollution origin of montenegrin surface waters, the first comprehensive study" in FEMS Online Conference on Microbiology 2020 (2020):403, https://hdl.handle.net/21.15107/rcub_rimsi_2161 .