TY  - CONF
AU  - Rodić, Ivanka
AU  - Lukičić, Jovana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2409
AB  - Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite
EP  - 233
SP  - 233
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2409
ER  - 

@conference{
author = "Rodić, Ivanka and Lukičić, Jovana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana",
year = "2022",
abstract = "Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite",
pages = "233-233",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2409"
}

Rodić, I., Lukičić, J., Stanić, M., Žižić, M., Zakrzewska, J., Živić, M.,& Cvetic Antic, T.. (2022). Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409

Rodić I, Lukičić J, Stanić M, Žižić M, Zakrzewska J, Živić M, Cvetic Antic T. Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

Rodić, Ivanka, Lukičić, Jovana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):233-233,
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2385
AB  - Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus
EP  - 858
SP  - 858
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2385
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2022",
abstract = "Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus",
pages = "858-858",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2385"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2022). Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):858-858,
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Cvetic Antic, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2113
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 96
SP  - 93
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2113
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Cvetic Antic, Tijana and Todorović, Nataša and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "96-93",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2113"
}

Žižić, M., Stanić, M., AQUILANTI, G., Cvetic Antic, T., Todorović, N., Živić, M.,& Zakrzewska, J.. (2022). SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113

Žižić M, Stanić M, AQUILANTI G, Cvetic Antic T, Todorović N, Živić M, Zakrzewska J. SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Cvetic Antic, Tijana, Todorović, Nataša, Živić, Miroslav, Zakrzewska, Joanna, "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):93-96,
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

TY  - JOUR
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Bajuk-Bogdanovic, Danica
AU  - Branković, Goran
AU  - Rodić, Ivanka
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106
AB  - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
PB  - Springer-Verlag GmbH Berlin Heidelberg
T2  - Analytical and Bianalytical Chemistry
T1  - Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus
EP  - 6222.
IS  - 20
SP  - 6213
VL  - 414
DO  - 10.1007/s00216-022-04191-4
ER  - 

@article{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy",
publisher = "Springer-Verlag GmbH Berlin Heidelberg",
journal = "Analytical and Bianalytical Chemistry",
title = "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus",
pages = "6222.-6213",
number = "20",
volume = "414",
doi = "10.1007/s00216-022-04191-4"
}

Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry
Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222..
https://doi.org/10.1007/s00216-022-04191-4

Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222..
doi:10.1007/s00216-022-04191-4 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222.,
https://doi.org/10.1007/s00216-022-04191-4 . .

TY  - CONF
AU  - Lukicic, J.
AU  - Rodic, I.
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1463
AB  - Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.
PB  - Wiley, Hoboken
C3  - Febs Open Bio
T1  - Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus
EP  - 397
SP  - 396
VL  - 11
DO  - 10.1002/2211-5463.13205
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1463
ER  - 

@conference{
author = "Lukicic, J. and Rodic, I. and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.",
publisher = "Wiley, Hoboken",
journal = "Febs Open Bio",
title = "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus",
pages = "397-396",
volume = "11",
doi = "10.1002/2211-5463.13205",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1463"
}

Lukicic, J., Rodic, I., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio
Wiley, Hoboken., 11, 396-397.
https://doi.org/10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463

Lukicic J, Rodic I, Žižić M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio. 2021;11:396-397.
doi:10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

Lukicic, J., Rodic, I., Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus" in Febs Open Bio, 11 (2021):396-397,
https://doi.org/10.1002/2211-5463.13205 .,
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

TY  - CONF
AU  - Žižić, Milan
AU  - Živić, Miroslav
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Zakrzewska, Joanna
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2203
AB  - Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great
potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus
truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The
submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR
spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of
vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome
vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR
measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite
containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced
through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexosephosphates.
Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the
fungal cell was confirmed.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021
C3  - 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.
T1  - INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM
EP  - 307
SP  - 304
DO  - 10.46793/ICCBI21.304Z
ER  - 

@conference{
author = "Žižić, Milan and Živić, Miroslav and Atlagić, Kristina and Karaman, Maja and Zakrzewska, Joanna",
year = "2021",
abstract = "Fungi could absorb heavy metals, metalloids, or radionuclides, thus fungal species possess great
potential in bioremediation. Since fungi absorb the vanadium, in the present study ability of Coprinellus
truncorum mycelia for vanadate uptake and its intracellular metabolism were investigated. The
submerged cultivated C. truncorum was exposed to a rising concentration of vanadate. 31P NMR
spectroscopy was used to investigate phosphate metabolism of the mycelium, while the status of
vanadium in the cell was followed by 51V NMR spectroscopy. The mycelium could grow, and overcome
vanadate presence, up to the concentration of 1.6 mM in the submerged medium. 31P NMR
measurements pointed out that vanadate induced changes in the concentration of the crucial metabolite
containing phosphorus, particularly sugar phosphates. The major result of vanadate action is evinced
through an appearance of a signal positioned at around 2.8 ppm, and an increased signal of hexosephosphates.
Using 51V NMR spectroscopy the presence of vanadate monomer in the mycelia of the
fungal cell was confirmed.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021",
journal = "1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.",
title = "INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM",
pages = "307-304",
doi = "10.46793/ICCBI21.304Z"
}

Žižić, M., Živić, M., Atlagić, K., Karaman, M.,& Zakrzewska, J.. (2021). INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM. in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2021., 304-307.
https://doi.org/10.46793/ICCBI21.304Z

Žižić M, Živić M, Atlagić K, Karaman M, Zakrzewska J. INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM. in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia.. 2021;:304-307.
doi:10.46793/ICCBI21.304Z .

Žižić, Milan, Živić, Miroslav, Atlagić, Kristina, Karaman, Maja, Zakrzewska, Joanna, "INFLUENCE OF VANADIUM ON THE GROWTH AND METABOLISM OF COPRINELLUS TRUNCORUM FUNGAL MYCELIUM" in 1st International Conference on Chemo and Bioinformatics, October 26-27, 2021. Kragujevac, Serbia. (2021):304-307,
https://doi.org/10.46793/ICCBI21.304Z . .

TY  - CONF
AU  - Žižić, Milan
AU  - Macura, Melanija
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1470
AB  - Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.204
ER  - 

@conference{
author = "Žižić, Milan and Macura, Melanija and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.204"
}

Žižić, M., Macura, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://doi.org/10.1016/j.freeradbiomed.2021.08.204

Žižić M, Macura M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine. 2021;177.
doi:10.1016/j.freeradbiomed.2021.08.204 .

Žižić, Milan, Macura, Melanija, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR" in Free Radical Biology and Medicine, 177 (2021),
https://doi.org/10.1016/j.freeradbiomed.2021.08.204 . .

TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
AB  - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
EP  - 3328
IS  - 3
SP  - 3320
VL  - 27
DO  - 10.1007/s11356-019-07200-9
ER  - 

@article{
author = "Zakrzewska, Joanna and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija",
year = "2020",
abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence",
pages = "3328-3320",
number = "3",
volume = "27",
doi = "10.1007/s11356-019-07200-9"
}

Zakrzewska, J., Mitrović, A. Lj., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(3), 3320-3328.
https://doi.org/10.1007/s11356-019-07200-9

Zakrzewska J, Mitrović AL, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328.
doi:10.1007/s11356-019-07200-9 .

Zakrzewska, Joanna, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328,
https://doi.org/10.1007/s11356-019-07200-9 . .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2418
AB  - Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
T1  - Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus
EP  - 118
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2418
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetic Antic, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2019",
abstract = "Vanadium enters mycelium of fungus P. blakesleeanus in both +5 and +4 oxidation states. Mycelium in three stages of growth (20h, 36h and 56h) was treated with three concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. The decrease in viability of mycelium was noticed only at 36h for all applied concentrations. Glutathione has a role in reduction of V+5 to a less toxic form V+4 , so we assayed the changes in total glutathione. Mycelium in early exponential phase of growth (20 h), after 1h or 5h treatment, showed concentration dependent decrease in glutathione, with the largest decrease of 40±4% after 1h and 22±9% after 5h treatment induced by 10 mM V+5. In stationary phase of growth (56h), changes in glutathione concentration were noticed only after 1h of treatment, and the largest decrease (36±6%) was induced by 1 mM V+5. Activities of glutathione reductase (GR), glutathione transferase (GST) and glutathione peroxidase (GPx), have also been examined. Increase in the activities of GPx and GST was noticed in 56h old mycelia treated for 5h with 5 mM and 10 mM V+5, but the only statistically significant increase (54±20%) was noticed in GST activity after 5h of 10 mM V+5 treatment.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia",
title = "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus",
pages = "118-118",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2418"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetic Antic, T., Živić, M.,& Stanić, M.. (2019). Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetic Antic T, Živić M, Stanić M. Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus. in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia. 2019;:118-118.
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetic Antic, Tijana, Živić, Miroslav, Stanić, Marina, "Effects of vanadate on glutathione metabolism in mycelium of fungus Phycomyces blakesleeanus" in Serbian Biochemical Society Ninth Conference, “Diversity in Biochemistry”. 14-16.11.2019. Belgrade, Serbia (2019):118-118,
https://hdl.handle.net/21.15107/rcub_rimsi_2418 .

TY  - CONF
AU  - Žižić, Milan
AU  - Tešanović, Kristina
AU  - Karaman, Maja
AU  - Zakrzewska, Joanna
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2207
AB  - Laetiporus sulphureus (Bull.) Murrill 1920, (Ph. Basidiomycota, Cl. Agaricomycetes, O. Polyporales, Fam. Polyporaceae) je jestiva gljiva, široko rasprostranjena u Evropi, Aziji i Severnoj Americi. Izolovana micelija autohtone gljive (vrba obala Dunava, Novi Sad) kultivisana je na čvrstoj podlozi i u potopljenom medijumu. Cilj istraživanja bio je da se upotrebom NMR spektroskopije ispita uticaj vanadijuma na metabolizam fosfata. Utvrđeno je da signali pozicionirani oko 4 ppm i 2,6 ppm pripadaju šećernim fosfatima (SP) i da nakon tretmana sa 10 mM vanadatom dolazi do povećanja signala za 78%, odnosno 23%. Nakon ispiranja micelijuma dolazi do postepene transformacije jednog oblika u drugi, tako da posle sat vremena signal SP na 2,6 ppm postaje dominantan u odnosu na signal pozicioniran na 4 ppm. Promene signala SP mogu ukazati na efektivnost inhibitora ili stimulatora energetskog metabolizma. Pored SP, polifosfati (PolyP) prisutni u miceliji gljive, takođe imaju ulogu u skladištenju energije. Primećeno je da se nakon izlaganja nepovoljnjim uslovima signal PolyP smanjio, što je potvrđeno u studijama sa kvascima S. cereviseae1 i N. crassa2. Nasuprot tome, nakon tretmana vanadijumom kod L. sulphureus nije došlo do redukcije signala PolyP, već je zabeležen mali porast signala dugolančanih polifosfata što može ukazati na mehanizam detoksifikacije micelije L. sulphureus kao metaboličkog odgovora micelije na dodavanje vanadata.
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus
EP  - 36
SP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2207
ER  - 

@conference{
author = "Žižić, Milan and Tešanović, Kristina and Karaman, Maja and Zakrzewska, Joanna",
year = "2018",
abstract = "Laetiporus sulphureus (Bull.) Murrill 1920, (Ph. Basidiomycota, Cl. Agaricomycetes, O. Polyporales, Fam. Polyporaceae) je jestiva gljiva, široko rasprostranjena u Evropi, Aziji i Severnoj Americi. Izolovana micelija autohtone gljive (vrba obala Dunava, Novi Sad) kultivisana je na čvrstoj podlozi i u potopljenom medijumu. Cilj istraživanja bio je da se upotrebom NMR spektroskopije ispita uticaj vanadijuma na metabolizam fosfata. Utvrđeno je da signali pozicionirani oko 4 ppm i 2,6 ppm pripadaju šećernim fosfatima (SP) i da nakon tretmana sa 10 mM vanadatom dolazi do povećanja signala za 78%, odnosno 23%. Nakon ispiranja micelijuma dolazi do postepene transformacije jednog oblika u drugi, tako da posle sat vremena signal SP na 2,6 ppm postaje dominantan u odnosu na signal pozicioniran na 4 ppm. Promene signala SP mogu ukazati na efektivnost inhibitora ili stimulatora energetskog metabolizma. Pored SP, polifosfati (PolyP) prisutni u miceliji gljive, takođe imaju ulogu u skladištenju energije. Primećeno je da se nakon izlaganja nepovoljnjim uslovima signal PolyP smanjio, što je potvrđeno u studijama sa kvascima S. cereviseae1 i N. crassa2. Nasuprot tome, nakon tretmana vanadijumom kod L. sulphureus nije došlo do redukcije signala PolyP, već je zabeležen mali porast signala dugolančanih polifosfata što može ukazati na mehanizam detoksifikacije micelije L. sulphureus kao metaboličkog odgovora micelije na dodavanje vanadata.",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus",
pages = "36-36",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2207"
}

Žižić, M., Tešanović, K., Karaman, M.,& Zakrzewska, J.. (2018). Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2207

Žižić M, Tešanović K, Karaman M, Zakrzewska J. Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:36-36.
https://hdl.handle.net/21.15107/rcub_rimsi_2207 .

Žižić, Milan, Tešanović, Kristina, Karaman, Maja, Zakrzewska, Joanna, "Uticaj vanadata na vremensko-zavisni metabolički odgovor micelije Laetiporus sulphureus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):36-36,
https://hdl.handle.net/21.15107/rcub_rimsi_2207 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2205
AB  - Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus
EP  - 33
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2205
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2018",
abstract = "Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2205"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2018). Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):33-33,
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Todorović, Nina
AU  - Zakrzewska, Joanna
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1161
AB  - Epinephrine (Epi) is a physiologically important catecholamine. Molecular conformation of Epi controls the interactions with other molecules and its biological effects. There have been a number of theoretical studies addressing conformation and hydrogen bonding of Epi in different solvents, but experimental data are scarce. Herein, we applied H-1 NMR, H-1-H-1 COSY, H-1-N-15 HSQC, and NOESY to examine and compare the conformation of Epi in polar solvents-dimethyl sulfoxide (DMSO) and water. The main differences were observed for NH2 and CH2 groups. Both showed chemical nonequivalence of protons in DMSO that was not present in water. The analysis of the effects of increasing temperature and solvent substitution on NMR signals showed that one of the protons in amine group forms a strong intramolecular hydrogen bond with aliphatic OH group, which is H-donor in another hydrogen bond with DMSO. NOESY provided data on the spatial positions of protons in the side chain, allowing for 3D model of the structure of Epi in DMSO to be built. In close, Epi molecule forms an additional 5-membered ring that encompasses bifurcate intra-/intermolecular hydrogen bonds, and acquires conformation that resembles the shape of a "scorpion"-the catechol ring representing the body and the side chain being a forward-curved tail. The conformation of Epi in water lacks the intramolecular hydrogen bond and most likely largely depends on hydrogen bonds with water molecules.
PB  - Springer/Plenum Publishers, New York
T2  - Structural Chemistry
T1  - The conformation of epinephrine in polar solvents: an NMR study
EP  - 1541
IS  - 5
SP  - 1533
VL  - 29
DO  - 10.1007/s11224-018-1144-y
ER  - 

@article{
author = "Korać Jačić, Jelena and Todorović, Nina and Zakrzewska, Joanna and Žižić, Milan and Spasojević, Ivan",
year = "2018",
abstract = "Epinephrine (Epi) is a physiologically important catecholamine. Molecular conformation of Epi controls the interactions with other molecules and its biological effects. There have been a number of theoretical studies addressing conformation and hydrogen bonding of Epi in different solvents, but experimental data are scarce. Herein, we applied H-1 NMR, H-1-H-1 COSY, H-1-N-15 HSQC, and NOESY to examine and compare the conformation of Epi in polar solvents-dimethyl sulfoxide (DMSO) and water. The main differences were observed for NH2 and CH2 groups. Both showed chemical nonequivalence of protons in DMSO that was not present in water. The analysis of the effects of increasing temperature and solvent substitution on NMR signals showed that one of the protons in amine group forms a strong intramolecular hydrogen bond with aliphatic OH group, which is H-donor in another hydrogen bond with DMSO. NOESY provided data on the spatial positions of protons in the side chain, allowing for 3D model of the structure of Epi in DMSO to be built. In close, Epi molecule forms an additional 5-membered ring that encompasses bifurcate intra-/intermolecular hydrogen bonds, and acquires conformation that resembles the shape of a "scorpion"-the catechol ring representing the body and the side chain being a forward-curved tail. The conformation of Epi in water lacks the intramolecular hydrogen bond and most likely largely depends on hydrogen bonds with water molecules.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Structural Chemistry",
title = "The conformation of epinephrine in polar solvents: an NMR study",
pages = "1541-1533",
number = "5",
volume = "29",
doi = "10.1007/s11224-018-1144-y"
}

Korać Jačić, J., Todorović, N., Zakrzewska, J., Žižić, M.,& Spasojević, I.. (2018). The conformation of epinephrine in polar solvents: an NMR study. in Structural Chemistry
Springer/Plenum Publishers, New York., 29(5), 1533-1541.
https://doi.org/10.1007/s11224-018-1144-y

Korać Jačić J, Todorović N, Zakrzewska J, Žižić M, Spasojević I. The conformation of epinephrine in polar solvents: an NMR study. in Structural Chemistry. 2018;29(5):1533-1541.
doi:10.1007/s11224-018-1144-y .

Korać Jačić, Jelena, Todorović, Nina, Zakrzewska, Joanna, Žižić, Milan, Spasojević, Ivan, "The conformation of epinephrine in polar solvents: an NMR study" in Structural Chemistry, 29, no. 5 (2018):1533-1541,
https://doi.org/10.1007/s11224-018-1144-y . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Tesanović, Kristina
AU  - Bosković, Eleonora
AU  - Nesović, Milica
AU  - Karaman, Maja
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1119
AB  - Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Effects of vanadate on the mycelium of edible fungus Coprinus comatus
EP  - 326
SP  - 320
VL  - 50
DO  - 10.1016/j.jtemb.2018.07.017
ER  - 

@article{
author = "Žižić, Milan and Zakrzewska, Joanna and Tesanović, Kristina and Bosković, Eleonora and Nesović, Milica and Karaman, Maja",
year = "2018",
abstract = "Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Effects of vanadate on the mycelium of edible fungus Coprinus comatus",
pages = "326-320",
volume = "50",
doi = "10.1016/j.jtemb.2018.07.017"
}

Žižić, M., Zakrzewska, J., Tesanović, K., Bosković, E., Nesović, M.,& Karaman, M.. (2018). Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology
Elsevier Gmbh, Munich., 50, 320-326.
https://doi.org/10.1016/j.jtemb.2018.07.017

Žižić M, Zakrzewska J, Tesanović K, Bosković E, Nesović M, Karaman M. Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology. 2018;50:320-326.
doi:10.1016/j.jtemb.2018.07.017 .

Žižić, Milan, Zakrzewska, Joanna, Tesanović, Kristina, Bosković, Eleonora, Nesović, Milica, Karaman, Maja, "Effects of vanadate on the mycelium of edible fungus Coprinus comatus" in Journal of Trace Elements in Medicine and Biology, 50 (2018):320-326,
https://doi.org/10.1016/j.jtemb.2018.07.017 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajic, Tanja
AU  - Ciric, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Todorović, Nataša
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1082
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
EP  - 372
IS  - 3
SP  - 364
VL  - 163
DO  - 10.1099/mic.0.000429
ER  - 

@article{
author = "Stanić, Marina and Krizak, Strahinja and Jovanović, Mirna and Pajic, Tanja and Ciric, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Todorović, Nataša and Zivić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
pages = "372-364",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429"
}

Stanić, M., Krizak, S., Jovanović, M., Pajic, T., Ciric, A., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Todorović, N.,& Zivić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm
Microbiology Soc, London., 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Krizak S, Jovanović M, Pajic T, Ciric A, Žižić M, Zakrzewska J, Cvetić-Antić T, Todorović N, Zivić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marina, Krizak, Strahinja, Jovanović, Mirna, Pajic, Tanja, Ciric, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Todorović, Nataša, Zivić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology-Sgm, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Hadzibrahimović, Mirzeta
AU  - Sužnjević, Desanka
AU  - Pastor, Ferenc
AU  - Cvetić-Antić, Tijana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1064
AB  - The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?
EP  - 373
IS  - 3
SP  - 365
VL  - 110
DO  - 10.1007/s10482-016-0808-0
ER  - 

@article{
author = "Hadzibrahimović, Mirzeta and Sužnjević, Desanka and Pastor, Ferenc and Cvetić-Antić, Tijana and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav",
year = "2017",
abstract = "The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?",
pages = "373-365",
number = "3",
volume = "110",
doi = "10.1007/s10482-016-0808-0"
}

Hadzibrahimović, M., Sužnjević, D., Pastor, F., Cvetić-Antić, T., Žižić, M., Zakrzewska, J.,& Zivić, M.. (2017). The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 110(3), 365-373.
https://doi.org/10.1007/s10482-016-0808-0

Hadzibrahimović M, Sužnjević D, Pastor F, Cvetić-Antić T, Žižić M, Zakrzewska J, Zivić M. The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2017;110(3):365-373.
doi:10.1007/s10482-016-0808-0 .

Hadzibrahimović, Mirzeta, Sužnjević, Desanka, Pastor, Ferenc, Cvetić-Antić, Tijana, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 110, no. 3 (2017):365-373,
https://doi.org/10.1007/s10482-016-0808-0 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Miladinović, Zoran P.
AU  - Stanić, Marina
AU  - Hadzibrahimović, Mirzeta
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/986
AB  - V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium
EP  - 528
IS  - 6
SP  - 521
VL  - 167
DO  - 10.1016/j.resmic.2016.04.012
ER  - 

@article{
author = "Žižić, Milan and Miladinović, Zoran P. and Stanić, Marina and Hadzibrahimović, Mirzeta and Zivić, Miroslav and Zakrzewska, Joanna",
year = "2016",
abstract = "V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium",
pages = "528-521",
number = "6",
volume = "167",
doi = "10.1016/j.resmic.2016.04.012"
}

Žižić, M., Miladinović, Z. P., Stanić, M., Hadzibrahimović, M., Zivić, M.,& Zakrzewska, J.. (2016). V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 167(6), 521-528.
https://doi.org/10.1016/j.resmic.2016.04.012

Žižić M, Miladinović ZP, Stanić M, Hadzibrahimović M, Zivić M, Zakrzewska J. V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology. 2016;167(6):521-528.
doi:10.1016/j.resmic.2016.04.012 .

Žižić, Milan, Miladinović, Zoran P., Stanić, Marina, Hadzibrahimović, Mirzeta, Zivić, Miroslav, Zakrzewska, Joanna, "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium" in Research in Microbiology, 167, no. 6 (2016):521-528,
https://doi.org/10.1016/j.resmic.2016.04.012 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Popovic-Bijelic, Ana
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Kalauzi, Aleksandar
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/950
AB  - Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.
PB  - Elsevier, Amsterdam
T2  - Applied Catalysis B-Environmental
T1  - Photo-redox reactions of indole and ferric iron in water
EP  - 180
SP  - 174
VL  - 185
DO  - 10.1016/j.apcatb.2015.12.018
ER  - 

@article{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Popovic-Bijelic, Ana and Zakrzewska, Joanna and Stanić, Marina and Kalauzi, Aleksandar and Spasojević, Ivan",
year = "2016",
abstract = "Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Catalysis B-Environmental",
title = "Photo-redox reactions of indole and ferric iron in water",
pages = "180-174",
volume = "185",
doi = "10.1016/j.apcatb.2015.12.018"
}

Milić Komić, S., Bogdanović Pristov, J., Popovic-Bijelic, A., Zakrzewska, J., Stanić, M., Kalauzi, A.,& Spasojević, I.. (2016). Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental
Elsevier, Amsterdam., 185, 174-180.
https://doi.org/10.1016/j.apcatb.2015.12.018

Milić Komić S, Bogdanović Pristov J, Popovic-Bijelic A, Zakrzewska J, Stanić M, Kalauzi A, Spasojević I. Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental. 2016;185:174-180.
doi:10.1016/j.apcatb.2015.12.018 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Popovic-Bijelic, Ana, Zakrzewska, Joanna, Stanić, Marina, Kalauzi, Aleksandar, Spasojević, Ivan, "Photo-redox reactions of indole and ferric iron in water" in Applied Catalysis B-Environmental, 185 (2016):174-180,
https://doi.org/10.1016/j.apcatb.2015.12.018 . .

TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikolić, Ljiljana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/869
AB  - We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
EP  - 173
IS  - 3
SP  - 162
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
ER  - 

@article{
author = "Krizak, Strahinja and Nikolić, Ljiljana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores",
pages = "173-162",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004"
}

Krizak, S., Nikolić, L., Stanić, M., Žižić, M., Zakrzewska, J., Zivić, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology
Elsevier, Amsterdam., 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikolić L, Stanić M, Žižić M, Zakrzewska J, Zivić M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikolić, Ljiljana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Ducic, Tanja
AU  - Grolimund, Daniel
AU  - Bajuk-Bogdanović, Danica
AU  - Nikolic, Miroslav
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Zakrzewska, Joanna
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/862
AB  - Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium
EP  - 7496
IS  - 24
SP  - 7487
VL  - 407
DO  - 10.1007/s00216-015-8916-7
ER  - 

@article{
author = "Žižić, Milan and Ducic, Tanja and Grolimund, Daniel and Bajuk-Bogdanović, Danica and Nikolic, Miroslav and Stanić, Marina and Krizak, Strahinja and Zakrzewska, Joanna",
year = "2015",
abstract = "Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium",
pages = "7496-7487",
number = "24",
volume = "407",
doi = "10.1007/s00216-015-8916-7"
}

Žižić, M., Ducic, T., Grolimund, D., Bajuk-Bogdanović, D., Nikolic, M., Stanić, M., Krizak, S.,& Zakrzewska, J.. (2015). X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 407(24), 7487-7496.
https://doi.org/10.1007/s00216-015-8916-7

Žižić M, Ducic T, Grolimund D, Bajuk-Bogdanović D, Nikolic M, Stanić M, Krizak S, Zakrzewska J. X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry. 2015;407(24):7487-7496.
doi:10.1007/s00216-015-8916-7 .

Žižić, Milan, Ducic, Tanja, Grolimund, Daniel, Bajuk-Bogdanović, Danica, Nikolic, Miroslav, Stanić, Marina, Krizak, Strahinja, Zakrzewska, Joanna, "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium" in Analytical and Bioanalytical Chemistry, 407, no. 24 (2015):7487-7496,
https://doi.org/10.1007/s00216-015-8916-7 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta
AU  - Pajdic, Aleksandra
AU  - Krizak, Strahinja
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/809
AB  - The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus
EP  - 857
IS  - 2
SP  - 847
VL  - 66
DO  - 10.2298/ABS1402847S
ER  - 

@article{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta and Pajdic, Aleksandra and Krizak, Strahinja and Žižić, Milan and Zakrzewska, Joanna",
year = "2014",
abstract = "The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus",
pages = "857-847",
number = "2",
volume = "66",
doi = "10.2298/ABS1402847S"
}

Stanić, M., Zivić, M., Hadzibrahimović, M., Pajdic, A., Krizak, S., Žižić, M.,& Zakrzewska, J.. (2014). Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 847-857.
https://doi.org/10.2298/ABS1402847S

Stanić M, Zivić M, Hadzibrahimović M, Pajdic A, Krizak S, Žižić M, Zakrzewska J. Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences. 2014;66(2):847-857.
doi:10.2298/ABS1402847S .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta, Pajdic, Aleksandra, Krizak, Strahinja, Žižić, Milan, Zakrzewska, Joanna, "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus" in Archives of Biological Sciences, 66, no. 2 (2014):847-857,
https://doi.org/10.2298/ABS1402847S . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Maksimović, Vuk
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/750
AB  - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
IS  - 7
VL  - 9
DO  - 10.1371/journal.pone.0102849
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2014",
abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus",
number = "7",
volume = "9",
doi = "10.1371/journal.pone.0102849"
}

Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One
Public Library Science, San Francisco., 9(7).
https://doi.org/10.1371/journal.pone.0102849

Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7).
doi:10.1371/journal.pone.0102849 .

Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014),
https://doi.org/10.1371/journal.pone.0102849 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zakrzewska, Joanna
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Marković, Zoran S
AU  - Vučinić, Željko
AU  - Zivić, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/658
AB  - Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism
EP  - 778
IS  - 7
SP  - 770
VL  - 164
DO  - 10.1016/j.resmic.2013.03.002
ER  - 

@article{
author = "Stanić, Marina and Zakrzewska, Joanna and Hadzibrahimović, Mirzeta R and Žižić, Milan and Marković, Zoran S and Vučinić, Željko and Zivić, Miroslav",
year = "2013",
abstract = "Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism",
pages = "778-770",
number = "7",
volume = "164",
doi = "10.1016/j.resmic.2013.03.002"
}

Stanić, M., Zakrzewska, J., Hadzibrahimović, M. R., Žižić, M., Marković, Z. S., Vučinić, Ž.,& Zivić, M.. (2013). Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology
Elsevier, Amsterdam., 164(7), 770-778.
https://doi.org/10.1016/j.resmic.2013.03.002

Stanić M, Zakrzewska J, Hadzibrahimović MR, Žižić M, Marković ZS, Vučinić Ž, Zivić M. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology. 2013;164(7):770-778.
doi:10.1016/j.resmic.2013.03.002 .

Stanić, Marina, Zakrzewska, Joanna, Hadzibrahimović, Mirzeta R, Žižić, Milan, Marković, Zoran S, Vučinić, Željko, Zivić, Miroslav, "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism" in Research in Microbiology, 164, no. 7 (2013):770-778,
https://doi.org/10.1016/j.resmic.2013.03.002 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/632
AB  - The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects
EP  - 69
IS  - 1
SP  - 61
VL  - 164
DO  - 10.1016/j.resmic.2012.08.007
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Spasojević, Ivan and Bogdanović Pristov, Jelena and Stanić, Marina and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2013",
abstract = "The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects",
pages = "69-61",
number = "1",
volume = "164",
doi = "10.1016/j.resmic.2012.08.007"
}

Žižić, M., Zivić, M., Spasojević, I., Bogdanović Pristov, J., Stanić, M., Cvetić-Antić, T.,& Zakrzewska, J.. (2013). The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 164(1), 61-69.
https://doi.org/10.1016/j.resmic.2012.08.007

Žižić M, Zivić M, Spasojević I, Bogdanović Pristov J, Stanić M, Cvetić-Antić T, Zakrzewska J. The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology. 2013;164(1):61-69.
doi:10.1016/j.resmic.2012.08.007 .

Žižić, Milan, Zivić, Miroslav, Spasojević, Ivan, Bogdanović Pristov, Jelena, Stanić, Marina, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects" in Research in Microbiology, 164, no. 1 (2013):61-69,
https://doi.org/10.1016/j.resmic.2012.08.007 . .

TY  - JOUR
AU  - Nikolić, Ljiljana M.
AU  - Todorović, Nataša
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Raus, Snežana Z
AU  - Kalauzi, Aleksandar
AU  - Janac, Branka B
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/539
AB  - The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.
PB  - Springer, New York
T2  - Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
T1  - Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field
EP  - 540
IS  - 7
SP  - 525
VL  - 198
DO  - 10.1007/s00359-012-0727-0
ER  - 

@article{
author = "Nikolić, Ljiljana M. and Todorović, Nataša and Zakrzewska, Joanna and Stanić, Marina and Raus, Snežana Z and Kalauzi, Aleksandar and Janac, Branka B",
year = "2012",
abstract = "The spontaneously active Br neuron from the brain-subesophageal ganglion complex of the garden snail Helix pomatia rhythmically generates regular bursts of action potentials with quiescent intervals accompanied by slow oscillations of membrane potential. We examined the involvement of the Na+/K+ pump in modulating its bursting activity by applying a static magnetic field. Whole snail brains and Br neuron were exposed to the 10-mT static magnetic field for 15 min. Biochemical data showed that Na+/K+-ATPase activity increased almost twofold after exposure of snail brains to the static magnetic field. Similarly, P-31 NMR data revealed a trend of increasing ATP consumption and increase in intracellular pH mediated by the Na+/H+ exchanger in snail brains exposed to the static magnetic field. Importantly, current clamp recordings from the Br neuron confirmed the increase in activity of the Na+/K+ pump after exposure to the static magnetic field, as the magnitude of ouabain's effect measured on the membrane resting potential, action potential, and interspike interval duration was higher in neurons exposed to the magnetic field. Metabolic pathways through which the magnetic field influenced the Na+/K+ pump could involve phosphorylation and dephosphorylation, as blocking these processes abolished the effect of the static magnetic field.",
publisher = "Springer, New York",
journal = "Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology",
title = "Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field",
pages = "540-525",
number = "7",
volume = "198",
doi = "10.1007/s00359-012-0727-0"
}

Nikolić, L. M., Todorović, N., Zakrzewska, J., Stanić, M., Raus, S. Z., Kalauzi, A.,& Janac, B. B.. (2012). Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology
Springer, New York., 198(7), 525-540.
https://doi.org/10.1007/s00359-012-0727-0

Nikolić LM, Todorović N, Zakrzewska J, Stanić M, Raus SZ, Kalauzi A, Janac BB. Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field. in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology. 2012;198(7):525-540.
doi:10.1007/s00359-012-0727-0 .

Nikolić, Ljiljana M., Todorović, Nataša, Zakrzewska, Joanna, Stanić, Marina, Raus, Snežana Z, Kalauzi, Aleksandar, Janac, Branka B, "Involvement of Na+/K(+)pump in fine modulation of bursting activity of the snail Br neuron by 10 mT static magnetic field" in Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioral Physiology, 198, no. 7 (2012):525-540,
https://doi.org/10.1007/s00359-012-0727-0 . .

TY  - GEN
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/497
AB  - Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus
EP  - 186
SP  - 186
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_497
ER  - 

@misc{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta R and Žižić, Milan and Zakrzewska, Joanna",
year = "2011",
abstract = "Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus",
pages = "186-186",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_497"
}

Stanić, M., Zivić, M., Hadzibrahimović, M. R., Žižić, M.,& Zakrzewska, J.. (2011). Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters
Springer, New York., 40, 186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497

Stanić M, Zivić M, Hadzibrahimović MR, Žižić M, Zakrzewska J. Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters. 2011;40:186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497 .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta R, Žižić, Milan, Zakrzewska, Joanna, "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus" in European Biophysics Journal With Biophysics Letters, 40 (2011):186-186,
https://hdl.handle.net/21.15107/rcub_rimsi_497 .