X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium
Само за регистроване кориснике
2015
Аутори
Žižić, MilanDucic, Tanja
Grolimund, Daniel
Bajuk-Bogdanović, Danica
Nikolic, Miroslav
Stanić, Marina
Krizak, Strahinja
Zakrzewska, Joanna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, a...nd potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.
Кључне речи:
X-ray absorption near-edge structure / Vanadium / Raman spectroscopy / Phycomyces blakesleeanusИзвор:
Analytical and Bioanalytical Chemistry, 2015, 407, 24, 7487-7496Издавач:
- Springer Heidelberg, Heidelberg
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
- Минерални стрес и адаптације биљака на маргиналним пољопривредним земљиштима (RS-MESTD-Basic Research (BR or ON)-173028)
DOI: 10.1007/s00216-015-8916-7
ISSN: 1618-2642
PubMed: 26253227
WoS: 000361396300023
Scopus: 2-s2.0-85027929937
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Ducic, Tanja AU - Grolimund, Daniel AU - Bajuk-Bogdanović, Danica AU - Nikolic, Miroslav AU - Stanić, Marina AU - Krizak, Strahinja AU - Zakrzewska, Joanna PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/862 AB - Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium. PB - Springer Heidelberg, Heidelberg T2 - Analytical and Bioanalytical Chemistry T1 - X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium EP - 7496 IS - 24 SP - 7487 VL - 407 DO - 10.1007/s00216-015-8916-7 ER -
@article{ author = "Žižić, Milan and Ducic, Tanja and Grolimund, Daniel and Bajuk-Bogdanović, Danica and Nikolic, Miroslav and Stanić, Marina and Krizak, Strahinja and Zakrzewska, Joanna", year = "2015", abstract = "Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.", publisher = "Springer Heidelberg, Heidelberg", journal = "Analytical and Bioanalytical Chemistry", title = "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium", pages = "7496-7487", number = "24", volume = "407", doi = "10.1007/s00216-015-8916-7" }
Žižić, M., Ducic, T., Grolimund, D., Bajuk-Bogdanović, D., Nikolic, M., Stanić, M., Krizak, S.,& Zakrzewska, J.. (2015). X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry Springer Heidelberg, Heidelberg., 407(24), 7487-7496. https://doi.org/10.1007/s00216-015-8916-7
Žižić M, Ducic T, Grolimund D, Bajuk-Bogdanović D, Nikolic M, Stanić M, Krizak S, Zakrzewska J. X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry. 2015;407(24):7487-7496. doi:10.1007/s00216-015-8916-7 .
Žižić, Milan, Ducic, Tanja, Grolimund, Daniel, Bajuk-Bogdanović, Danica, Nikolic, Miroslav, Stanić, Marina, Krizak, Strahinja, Zakrzewska, Joanna, "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium" in Analytical and Bioanalytical Chemistry, 407, no. 24 (2015):7487-7496, https://doi.org/10.1007/s00216-015-8916-7 . .