TY  - JOUR
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Tesanović, Kristina
AU  - Bosković, Eleonora
AU  - Nesović, Milica
AU  - Karaman, Maja
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1119
AB  - Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Effects of vanadate on the mycelium of edible fungus Coprinus comatus
EP  - 326
SP  - 320
VL  - 50
DO  - 10.1016/j.jtemb.2018.07.017
ER  - 

@article{
author = "Žižić, Milan and Zakrzewska, Joanna and Tesanović, Kristina and Bosković, Eleonora and Nesović, Milica and Karaman, Maja",
year = "2018",
abstract = "Vanadate is proposed to play a pivotal role in application of edible fungus Coprinus comatus for medical purposes. In this study the concentration of extracellular vanadate acceptable for the submerged cultivation of C. comatus mycelium was established. The mycelium could grow, and overcome vanadate toxic effects, up to the concentration of 3.3 mM. Moreover, in this condition, at the end of the exponential phase of growth, biomass yield was almost identical to that in the control. P-31 NMR spectroscopy showed that addition of 10 mM vanadate to the mycelium in the exponential phase of growth provoked instantaneous increase of a sugar phosphates level which could be related to changes in activities of glycolytic enzymes. Exposure to higher vanadate concentration was toxic for the cell. V-51 NMR measurements revealed that monomer of vanadate is present in the cytoplasm causing the metabolic changes. C. comatus has also capacity for vanadate reduction, as shown by EPR measurements, but vanadyl uptake is significantly less comparing to vanadate.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Effects of vanadate on the mycelium of edible fungus Coprinus comatus",
pages = "326-320",
volume = "50",
doi = "10.1016/j.jtemb.2018.07.017"
}

Žižić, M., Zakrzewska, J., Tesanović, K., Bosković, E., Nesović, M.,& Karaman, M.. (2018). Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology
Elsevier Gmbh, Munich., 50, 320-326.
https://doi.org/10.1016/j.jtemb.2018.07.017

Žižić M, Zakrzewska J, Tesanović K, Bosković E, Nesović M, Karaman M. Effects of vanadate on the mycelium of edible fungus Coprinus comatus. in Journal of Trace Elements in Medicine and Biology. 2018;50:320-326.
doi:10.1016/j.jtemb.2018.07.017 .

Žižić, Milan, Zakrzewska, Joanna, Tesanović, Kristina, Bosković, Eleonora, Nesović, Milica, Karaman, Maja, "Effects of vanadate on the mycelium of edible fungus Coprinus comatus" in Journal of Trace Elements in Medicine and Biology, 50 (2018):320-326,
https://doi.org/10.1016/j.jtemb.2018.07.017 . .