Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism
Само за регистроване кориснике
2013
Аутори
Stanić, MarinaZakrzewska, Joanna
Hadzibrahimović, Mirzeta R
Žižić, Milan
Marković, Zoran S
Vučinić, Željko
Zivić, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to susta...in energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
Кључне речи:
Polyphosphates / Phycomyces blakesleeanus / Anoxia / Alternative oxidaseИзвор:
Research in Microbiology, 2013, 164, 7, 770-778Издавач:
- Elsevier, Amsterdam
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1016/j.resmic.2013.03.002
ISSN: 0923-2508
PubMed: 23542427
WoS: 000324010800010
Scopus: 2-s2.0-84881549616
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Stanić, Marina AU - Zakrzewska, Joanna AU - Hadzibrahimović, Mirzeta R AU - Žižić, Milan AU - Marković, Zoran S AU - Vučinić, Željko AU - Zivić, Miroslav PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/658 AB - Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens. PB - Elsevier, Amsterdam T2 - Research in Microbiology T1 - Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism EP - 778 IS - 7 SP - 770 VL - 164 DO - 10.1016/j.resmic.2013.03.002 ER -
@article{ author = "Stanić, Marina and Zakrzewska, Joanna and Hadzibrahimović, Mirzeta R and Žižić, Milan and Marković, Zoran S and Vučinić, Željko and Zivić, Miroslav", year = "2013", abstract = "Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.", publisher = "Elsevier, Amsterdam", journal = "Research in Microbiology", title = "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism", pages = "778-770", number = "7", volume = "164", doi = "10.1016/j.resmic.2013.03.002" }
Stanić, M., Zakrzewska, J., Hadzibrahimović, M. R., Žižić, M., Marković, Z. S., Vučinić, Ž.,& Zivić, M.. (2013). Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology Elsevier, Amsterdam., 164(7), 770-778. https://doi.org/10.1016/j.resmic.2013.03.002
Stanić M, Zakrzewska J, Hadzibrahimović MR, Žižić M, Marković ZS, Vučinić Ž, Zivić M. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology. 2013;164(7):770-778. doi:10.1016/j.resmic.2013.03.002 .
Stanić, Marina, Zakrzewska, Joanna, Hadzibrahimović, Mirzeta R, Žižić, Milan, Marković, Zoran S, Vučinić, Željko, Zivić, Miroslav, "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism" in Research in Microbiology, 164, no. 7 (2013):770-778, https://doi.org/10.1016/j.resmic.2013.03.002 . .