Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
2014
Autori
Žižić, MilanZivić, Miroslav
Maksimović, Vuk
Stanić, Marina
Krizak, Strahinja
Cvetić-Antić, Tijana
Zakrzewska, Joanna
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e.,... phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
Ključne reči:
vanadium / fungi / V5+Izvor:
PLoS One, 2014, 9, 7Izdavač:
- Public Library Science, San Francisco
Finansiranje / projekti:
- Interakcije membrana sa unutarćelijskim i apoplastičnim prostorom: izučavanja bioenergetike i singalizacije koristeći biofizičke i biohemijske metode (RS-MESTD-Basic Research (BR or ON)-173040)
DOI: 10.1371/journal.pone.0102849
ISSN: 1932-6203
PubMed: 25036378
WoS: 000339615200090
Scopus: 2-s2.0-84904575923
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Zivić, Miroslav AU - Maksimović, Vuk AU - Stanić, Marina AU - Krizak, Strahinja AU - Cvetić-Antić, Tijana AU - Zakrzewska, Joanna PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/750 AB - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi. PB - Public Library Science, San Francisco T2 - PLoS One T1 - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus IS - 7 VL - 9 DO - 10.1371/journal.pone.0102849 ER -
@article{ author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna", year = "2014", abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.", publisher = "Public Library Science, San Francisco", journal = "PLoS One", title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus", number = "7", volume = "9", doi = "10.1371/journal.pone.0102849" }
Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One Public Library Science, San Francisco., 9(7). https://doi.org/10.1371/journal.pone.0102849
Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7). doi:10.1371/journal.pone.0102849 .
Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014), https://doi.org/10.1371/journal.pone.0102849 . .