TY  - JOUR
AU  - Žižić, Milan
AU  - Atlagić, Kristina
AU  - Karaman, Maja
AU  - Živić, Miroslav
AU  - Stanić, Marina
AU  - Maksimović, Vuk
AU  - Zakrzewska, Joanna
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3165
AB  - BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Trace Elements in Biology and Medicine
T1  - Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass
EP  - 127386
SP  - 127381
VL  - 83
DO  - 10.1016/j.jtemb.2024.127381
ER  - 

@article{
author = "Žižić, Milan and Atlagić, Kristina and Karaman, Maja and Živić, Miroslav and Stanić, Marina and Maksimović, Vuk and Zakrzewska, Joanna",
year = "2024",
abstract = "BACKGROUND
Fungi absorb and solubilize a broad spectrum of heavy metals such as vanadium (V), which makes them a main route of its entry into the biosphere. V as vanadate (V5+) is a potential medical agent due to its many metabolic actions such as interaction with phosphates in the cell, and especially its insulin-mimetic activity. Antidiabetic activity of V-enriched fungi has been studied in recent years, but the biological and chemical bases of vanadium action and status in fungi in general are poorly understood, with almost no information on edible fungi. 
METHODS
This manuscript gives a deeper insight into the interaction of V5+ with Coprinellus truncorum, an edible autochthonous species widely distributed in Europe and North America. Vanadium uptake and accumulation as V5+ was studied by 51V NMR, while the reducing abilities of the mycelium were determined by EPR. 31P NMR was used to determine its effects on the metabolism of phosphate compounds, with particular focus on phosphate sugars identified using HPLC.
RESULTS
Vanadate enters the mycelium in monomeric form and shows no immediate detrimental effects on intracellular pH or polyphosphate (PPc) levels, even when applied at physiologically high concentrations (20 mM Na3VO4). Once absorbed, it is partially reduced to less toxic vanadyl (V4+) with notable unreduced portion, which leads to a large increase in phosphorylated sugar levels, especially glucose-1-phosphate (G1P) and fructose-6-phosphate (F6P).
CONCLUSIONS
Preservation of pH and especially PPc reflects maintenance of the energy status of the mycelium, i.e., its tolerance to high V5+ concentrations. Rise in G1P and F6P levels implies that the main targets of V5+ are most likely phosphoglucomutase and phosphoglucokinase(s), enzymes involved in early stages of G6P transformation in glycolysis and glycogen metabolism. This study recommends C. truncorum for further investigation as a potential antidiabetic agent.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Trace Elements in Biology and Medicine",
title = "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass",
pages = "127386-127381",
volume = "83",
doi = "10.1016/j.jtemb.2024.127381"
}

Žižić, M., Atlagić, K., Karaman, M., Živić, M., Stanić, M., Maksimović, V.,& Zakrzewska, J.. (2024). Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine
Elsevier Gmbh, Munich., 83, 127381-127386.
https://doi.org/10.1016/j.jtemb.2024.127381

Žižić M, Atlagić K, Karaman M, Živić M, Stanić M, Maksimović V, Zakrzewska J. Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass. in Journal of Trace Elements in Biology and Medicine. 2024;83:127381-127386.
doi:10.1016/j.jtemb.2024.127381 .

Žižić, Milan, Atlagić, Kristina, Karaman, Maja, Živić, Miroslav, Stanić, Marina, Maksimović, Vuk, Zakrzewska, Joanna, "Uptake of vanadium and its intracellular metabolism by Coprinellus truncorum mycelial biomass" in Journal of Trace Elements in Biology and Medicine, 83 (2024):127381-127386,
https://doi.org/10.1016/j.jtemb.2024.127381 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Santrač, Isidora
AU  - Danilović Luković, Jelena
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Zechmann, Bernd
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3144
AB  - Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a
remarkable metabolic and structural adaptability that allows it to thrive in metal-infested
environments. H. pluvialis could be potentially used in the remediation of waters polluted
with metals, such as manganese (Mn). Mn is also an essential element involved in different
metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we
examined morphological response of metabolically active green cell type of H. pluvialis
(microzooids) to high Mn concentrations exceeding their physiological quota. When exposed
to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma
atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after
1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24
h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1
hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs
showed that some cells had wall rupture and degraded intracellular content and damaged
organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by
thickened cell wall, preserved intracellular compartments, and reduced total area of lipid
droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of
Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced
cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall
thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations
exemplified by cell wall thickening, reduction in lipid droplets total area due to increased
energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural
adaptability. Further investigation using analytical methods will provide a more profound
understanding of the metabolic dimensions of adaptive response
PB  - UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade
C3  - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
T1  - Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess
EP  - 44
SP  - 44
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3144
ER  - 

@conference{
author = "Santrač, Isidora and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Zechmann, Bernd and Žižić, Milan and Spasojević, Ivan",
year = "2023",
abstract = "Haematococcus pluvialis is a unicellular green alga with a complex life cycle and a
remarkable metabolic and structural adaptability that allows it to thrive in metal-infested
environments. H. pluvialis could be potentially used in the remediation of waters polluted
with metals, such as manganese (Mn). Mn is also an essential element involved in different
metabolic processes, such as photosynthesis and antioxidantive defence. Herein, we
examined morphological response of metabolically active green cell type of H. pluvialis
(microzooids) to high Mn concentrations exceeding their physiological quota. When exposed
to 1 mM Mn2+, cell viability remained stable over a 3-day period. Inductively coupled plasma
atomic emission spectroscopy showed the prompt uptake of Mn by the microzooid cells after
1 h of the treatment, with a modest increase of the concentration of Mn in the biomass at 24
h. Scanning electron microscopy revealed granular deposits on microzooid surfaces after 1
hour, likely Mn deposits, while transmission electron microscopy (TEM) micrographs
showed that some cells had wall rupture and degraded intracellular content and damaged
organelles. After 24 and 72 h, a different type of cell morphology emerged, characterized by
thickened cell wall, preserved intracellular compartments, and reduced total area of lipid
droplets. Both cell types exhibited vacuoles containing dark granules, possibly indicative of
Mn accumulations. Quantitative TEM analysis demonstrated that an excess of Mn reduced
cell cross-section and lipid droplet area while increasing vacuole cross-section and cell wall
thickness. The intricate adaptive responses of H. pluvialis to elevated Mn concentrations
exemplified by cell wall thickening, reduction in lipid droplets total area due to increased
energy demand, and the accumulation of Mn in vacuoles, exhibits the impressive structural
adaptability. Further investigation using analytical methods will provide a more profound
understanding of the metabolic dimensions of adaptive response",
publisher = "UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade",
journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia",
title = "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess",
pages = "44-44",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3144"
}

Santrač, I., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S., Zechmann, B., Žižić, M.,& Spasojević, I.. (2023). Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
UNIVERSITY OF BELGRADE, FACULTY OF TECHNOLOGY AND METALLURGY Karnegijeva 4, Belgrade., 44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3144

Santrač I, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Zechmann B, Žižić M, Spasojević I. Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:44-44.
https://hdl.handle.net/21.15107/rcub_rimsi_3144 .

Santrač, Isidora, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Zechmann, Bernd, Žižić, Milan, Spasojević, Ivan, "Structural adaptibility of Haematococcus pluvialis green phase cells exposed to manganese excess" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):44-44,
https://hdl.handle.net/21.15107/rcub_rimsi_3144 .

TY  - CONF
AU  - Santrač, Isidora
AU  - Žižić, Milan
AU  - AQUILANTI, Giuliana
AU  - Gianoncelli, Alessandra
AU  - Bonanni, Valentina
AU  - Danilović Luković, Jelena
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3139
AB  - Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.
C3  - International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia
T1  - The coordination and storage of manganese in the microalga Haematococcus pluvialis
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3139
ER  - 

@conference{
author = "Santrač, Isidora and Žižić, Milan and AQUILANTI, Giuliana and Gianoncelli, Alessandra and Bonanni, Valentina and Danilović Luković, Jelena and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Spasojević, Ivan",
year = "2023",
abstract = "Haematococcus pluvialis is a unicellular green alga of significant environmental and
economic impact, well adapted to life in extreme conditions such as metal pollution.
Manganese is a common pollutant of water bodies that is related to mining and industrial
drainage, and microalgae have been applied in their bioremediation. To study changes in Mn
redox and coordination form upon interaction with H. pluvialis cells, we exposed late
exponential/early stationary green phase culture to 1 mM MnCl2 (Mn2+) for 72 h. Applied
concentration exceeds microalgal physiological quota but it was non-toxic. Structure of
coordinated Mn in the cells was analyzed using X-ray absorption near edge structure
(XANES) and extended X-ray Absorption Fine Structure (EXAFS) spectroscopy, while
visualization and elemental mapping of Mn was performed by micro X-ray fluorescence
(μXRF). XANES spectra showed that the oxidation form of Mn in H. pluvialis biomass
remained 2+. However, EXAFS showed Mn coordination in microalgae is closer to hydrated
MnSO4 with minor deviation of the local geometry. The shorter Mn-O bonds in biomass,
compared to crystal model, implies somewhat more stable complex in H. pluvialis. This
suggests that H. pluvialis may use sulphated polysaccharides for Mn sequestration, which
would be a newly proposed mechanism of metal coordination and storage. μXRF analysis
showed co-localization of Mn with O and Na, with particularly good superimposition for Mn
and O. O-rich regions may represent vacuoles, filled with organic acids including sulphates,
or starch granules and accumulations of sulphated polysaccharides, which are known to be
produced by H. pluvialis. In addition, vacuoles in plant cells are known to act as sinks for
Na+ ions. These findings are in line with structural analysis that showed dark precipitates in
vacuoles, which are likely accumulations of Mn.",
journal = "International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia",
title = "The coordination and storage of manganese in the microalga Haematococcus pluvialis",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3139"
}

Santrač, I., Žižić, M., AQUILANTI, G., Gianoncelli, A., Bonanni, V., Danilović Luković, J., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S.,& Spasojević, I.. (2023). The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia, 45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139

Santrač I, Žižić M, AQUILANTI G, Gianoncelli A, Bonanni V, Danilović Luković J, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Spasojević I. The coordination and storage of manganese in the microalga Haematococcus pluvialis. in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia. 2023;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

Santrač, Isidora, Žižić, Milan, AQUILANTI, Giuliana, Gianoncelli, Alessandra, Bonanni, Valentina, Danilović Luković, Jelena, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Spasojević, Ivan, "The coordination and storage of manganese in the microalga Haematococcus pluvialis" in International Conference on Biochemical Engineering and Biotechnology for Young Scientists, December 7-8th 2023, Belgrade, Serbia (2023):45,
https://hdl.handle.net/21.15107/rcub_rimsi_3139 .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Kovačević, Snežana
AU  - Jovanović, Uroš
AU  - Stanić, Marina
AU  - Opačić, Miloš
AU  - Santrač, Isidora
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2421
AB  - Heavy metals remain a major pollutant in waters near mining sites. Water pollution is a current, longterm problem that affects plants and organisms that live in these water systems, and the effect is very harmful not only for individual species and populations, but also for the entire biological community. This study analyzed the potential of the microalga Chlorella sorokiniana in the adsorption of four selected metals: Fe, Cu, Zn and Ni, all present in high amounts in Lake Robule. This pilot study was conducted to evaluate the potential of these microalgae for possible use in future bioremediation treatment of these water. A laboratory study of metal accumulation in lake water samples lasted for 7 days, after which a total decrease in metal concentration was observed, namely Fe ~25%, Cu ~17%, and Zn ~4% on the seventh day. This study confirms the strong potential of microalgae C. sorokiniana to reduce the presence of heavy metals in conditions known for the low pH value of water with a high percentage of Fe and other heavy metals.
PB  - University of Belgrade
PB  - Technical Faculty in Bor
C3  - 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia
T1  - Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule
EP  - 334
SP  - 330
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2421
ER  - 

@conference{
author = "Dimitrijević, Milena and Kovačević, Snežana and Jovanović, Uroš and Stanić, Marina and Opačić, Miloš and Santrač, Isidora and Tanović, Marija and Ćurić, Valentina and Spasojević, Ivan",
year = "2023",
abstract = "Heavy metals remain a major pollutant in waters near mining sites. Water pollution is a current, longterm problem that affects plants and organisms that live in these water systems, and the effect is very harmful not only for individual species and populations, but also for the entire biological community. This study analyzed the potential of the microalga Chlorella sorokiniana in the adsorption of four selected metals: Fe, Cu, Zn and Ni, all present in high amounts in Lake Robule. This pilot study was conducted to evaluate the potential of these microalgae for possible use in future bioremediation treatment of these water. A laboratory study of metal accumulation in lake water samples lasted for 7 days, after which a total decrease in metal concentration was observed, namely Fe ~25%, Cu ~17%, and Zn ~4% on the seventh day. This study confirms the strong potential of microalgae C. sorokiniana to reduce the presence of heavy metals in conditions known for the low pH value of water with a high percentage of Fe and other heavy metals.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia",
title = "Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule",
pages = "334-330",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2421"
}

Dimitrijević, M., Kovačević, S., Jovanović, U., Stanić, M., Opačić, M., Santrač, I., Tanović, M., Ćurić, V.,& Spasojević, I.. (2023). Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule. in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia
University of Belgrade., 330-334.
https://hdl.handle.net/21.15107/rcub_rimsi_2421

Dimitrijević M, Kovačević S, Jovanović U, Stanić M, Opačić M, Santrač I, Tanović M, Ćurić V, Spasojević I. Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule. in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia. 2023;:330-334.
https://hdl.handle.net/21.15107/rcub_rimsi_2421 .

Dimitrijević, Milena, Kovačević, Snežana, Jovanović, Uroš, Stanić, Marina, Opačić, Miloš, Santrač, Isidora, Tanović, Marija, Ćurić, Valentina, Spasojević, Ivan, "Application of microalga Chlorella sorokiniana in wastewater bioremediation – case of lake Robule" in 30th international conference ecological truth and environmental research – EcoTER’23. 20-23 June 2023, Mt Stara Planina, Serbia (2023):330-334,
https://hdl.handle.net/21.15107/rcub_rimsi_2421 .

TY  - CONF
AU  - Santrač, Isidora
AU  - Dimitrijević, Milena
AU  - Stanić, Marina
AU  - Tanović, Marija
AU  - Ćurić, Valentina
AU  - Kovačević, Snežana
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2416
AB  - Acid mine drainage ponds represents a specific artificial ecosystem that gives advantage to extemophilic microalgae. The mechanisms of adaptaton of such strains to excess metal concentrations that are common for their habitat, are poorly understood. Herein, we analzyed two strains of the green microalga Chlamydomonas acidophila - 137 and PM01, which have been isolated from different mining sites, for their interactions with Mn ions. The effects of different concentrations of Mn2+ were investigated in the late exponential/early stationary phase of culture growth (15 days). Viability was determined by Evans blue assay. No toxic effects were observed at concentrations as high as 2 mM Mn2+. The the time dynamics of Mn accumulation in the biomass was determined using ICP. It was shown that the maximum accumulation of Mn in strain 137 (3.79 ± 0.68 μg/mg) was reached at 24h, while for PM01 the highest uptake (3.23 ± 0.17 μg/mg) was observed at 72 h. Next we analyzed redox settings by measuring the levels of reduced thiols unsing in vivo EPR spin probing. The treatment with 2 mM Mn2+ induced a rapid and irreversible decrease in the level of thiols which indicates that Mn2+ activated prooxidtaive processes. The maximum drop of thios levels were: from 4.80 ± 0.05 to 2.70 ± 0.05nmol/mg fresh weight after 1h for 137; and from 4.70 ± 0.05 to 3.40 ± 0.05 nmol/mg fresh weiht after 30 min for PM01. Further, the level of reactive oxygen species was evaluated using fluorescent probe DCFH-DA assay. The changes were in agreement with thiol levels. Both tested algal strains show resistance to Mn and are therefore good candidates for application in water bioremediation.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference, “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia
T1  - The accumulation of manganese by Chlamydomonas acidophila strains isolated from acid mine drainage
EP  - 115
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2416
ER  - 

@conference{
author = "Santrač, Isidora and Dimitrijević, Milena and Stanić, Marina and Tanović, Marija and Ćurić, Valentina and Kovačević, Snežana and Spasojević, Ivan",
year = "2023",
abstract = "Acid mine drainage ponds represents a specific artificial ecosystem that gives advantage to extemophilic microalgae. The mechanisms of adaptaton of such strains to excess metal concentrations that are common for their habitat, are poorly understood. Herein, we analzyed two strains of the green microalga Chlamydomonas acidophila - 137 and PM01, which have been isolated from different mining sites, for their interactions with Mn ions. The effects of different concentrations of Mn2+ were investigated in the late exponential/early stationary phase of culture growth (15 days). Viability was determined by Evans blue assay. No toxic effects were observed at concentrations as high as 2 mM Mn2+. The the time dynamics of Mn accumulation in the biomass was determined using ICP. It was shown that the maximum accumulation of Mn in strain 137 (3.79 ± 0.68 μg/mg) was reached at 24h, while for PM01 the highest uptake (3.23 ± 0.17 μg/mg) was observed at 72 h. Next we analyzed redox settings by measuring the levels of reduced thiols unsing in vivo EPR spin probing. The treatment with 2 mM Mn2+ induced a rapid and irreversible decrease in the level of thiols which indicates that Mn2+ activated prooxidtaive processes. The maximum drop of thios levels were: from 4.80 ± 0.05 to 2.70 ± 0.05nmol/mg fresh weight after 1h for 137; and from 4.70 ± 0.05 to 3.40 ± 0.05 nmol/mg fresh weiht after 30 min for PM01. Further, the level of reactive oxygen species was evaluated using fluorescent probe DCFH-DA assay. The changes were in agreement with thiol levels. Both tested algal strains show resistance to Mn and are therefore good candidates for application in water bioremediation.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference, “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia",
title = "The accumulation of manganese by Chlamydomonas acidophila strains isolated from acid mine drainage",
pages = "115-115",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2416"
}

Santrač, I., Dimitrijević, M., Stanić, M., Tanović, M., Ćurić, V., Kovačević, S.,& Spasojević, I.. (2023). The accumulation of manganese by Chlamydomonas acidophila strains isolated from acid mine drainage. in Serbian Biochemical Society Twelfth Conference, “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 115-115.
https://hdl.handle.net/21.15107/rcub_rimsi_2416

Santrač I, Dimitrijević M, Stanić M, Tanović M, Ćurić V, Kovačević S, Spasojević I. The accumulation of manganese by Chlamydomonas acidophila strains isolated from acid mine drainage. in Serbian Biochemical Society Twelfth Conference, “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia. 2023;:115-115.
https://hdl.handle.net/21.15107/rcub_rimsi_2416 .

Santrač, Isidora, Dimitrijević, Milena, Stanić, Marina, Tanović, Marija, Ćurić, Valentina, Kovačević, Snežana, Spasojević, Ivan, "The accumulation of manganese by Chlamydomonas acidophila strains isolated from acid mine drainage" in Serbian Biochemical Society Twelfth Conference, “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia (2023):115-115,
https://hdl.handle.net/21.15107/rcub_rimsi_2416 .

TY  - CONF
AU  - Tanović, Marija
AU  - Dimitrijević, Milena
AU  - Milenković, Milica
AU  - Stanić, Marina
AU  - Jagličić, Zvonko
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2699
AB  - Metabolism of metals in microalgae, as well as their adaptation to metal excess, are of significant environmental importance. We have found previously that the green microalga Chlorella sorokiniana accumulates environmental Mn excess in the form of a multivalent Mn-O-Ca cluster with structure that is very similar of oxygen-evolving complex (OEC) in photosystem II1. The application of microalgae in the ‘green’ synthesis of catalytic metal clusters is very important, since the use of toxic chemicals for traditional synthesis could be avoided. The aim of this study was to investigate the magnetic, redox and structural properties of this cluster. The magnetic properties of the cluster, were tested using Low-T-EPR spectroscopy and SQUID magnetometry. Based on the analysis of the EPR spectra, it was concluded that the spin of Mn in the cluster is >1/2. In addition, the spin of Mn is not an integer since parallel mode EPR did not deliver any detectable spectrum. The paramagnetic nature of the Mn-O-Ca cluster was confirmed by the SQUID instrument. Effective magnetic moment calculated per ion Mn was ~5 BM (Mn ratio in biomass was 12,1%). For the investigation of redox and structural properties, an extract of cluster from microalga was used. Cluster extraction involved the application of a series of solvents - phenol in Tris-Cl buffer, chloroform, methanol, 0,17% sodium hypochlorite2. Cyclic and differential voltagrams were recorded in the range from -1,5 to 2 mV3. The analysis revealed the presence of different oxidation states of Mn (+2, +3, +4). The peak potentials resembled the potentials in OEC model compounds2.The redox similarities between the Mn-O-Ca cluster in microalgae and OEC imply that cluster may be have a similar catalytic activity. More detailed analysis of activity and structure of Mn-O-Ca cluster is warranted.
PB  - Serbian Biochemical Society
C3  - Serbian Biochemical Society Twelfth Conference “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia
T1  - Magnetic, redox and structural properties of Mn-O-Ca cluster, synthesized by the green microalga Chlorella sorokiniana
EP  - 125
SP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2699
ER  - 

@conference{
author = "Tanović, Marija and Dimitrijević, Milena and Milenković, Milica and Stanić, Marina and Jagličić, Zvonko and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae, as well as their adaptation to metal excess, are of significant environmental importance. We have found previously that the green microalga Chlorella sorokiniana accumulates environmental Mn excess in the form of a multivalent Mn-O-Ca cluster with structure that is very similar of oxygen-evolving complex (OEC) in photosystem II1. The application of microalgae in the ‘green’ synthesis of catalytic metal clusters is very important, since the use of toxic chemicals for traditional synthesis could be avoided. The aim of this study was to investigate the magnetic, redox and structural properties of this cluster. The magnetic properties of the cluster, were tested using Low-T-EPR spectroscopy and SQUID magnetometry. Based on the analysis of the EPR spectra, it was concluded that the spin of Mn in the cluster is >1/2. In addition, the spin of Mn is not an integer since parallel mode EPR did not deliver any detectable spectrum. The paramagnetic nature of the Mn-O-Ca cluster was confirmed by the SQUID instrument. Effective magnetic moment calculated per ion Mn was ~5 BM (Mn ratio in biomass was 12,1%). For the investigation of redox and structural properties, an extract of cluster from microalga was used. Cluster extraction involved the application of a series of solvents - phenol in Tris-Cl buffer, chloroform, methanol, 0,17% sodium hypochlorite2. Cyclic and differential voltagrams were recorded in the range from -1,5 to 2 mV3. The analysis revealed the presence of different oxidation states of Mn (+2, +3, +4). The peak potentials resembled the potentials in OEC model compounds2.The redox similarities between the Mn-O-Ca cluster in microalgae and OEC imply that cluster may be have a similar catalytic activity. More detailed analysis of activity and structure of Mn-O-Ca cluster is warranted.",
publisher = "Serbian Biochemical Society",
journal = "Serbian Biochemical Society Twelfth Conference “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia",
title = "Magnetic, redox and structural properties of Mn-O-Ca cluster, synthesized by the green microalga Chlorella sorokiniana",
pages = "125-125",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2699"
}

Tanović, M., Dimitrijević, M., Milenković, M., Stanić, M., Jagličić, Z.,& Spasojević, I.. (2023). Magnetic, redox and structural properties of Mn-O-Ca cluster, synthesized by the green microalga Chlorella sorokiniana. in Serbian Biochemical Society Twelfth Conference “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia
Serbian Biochemical Society., 125-125.
https://hdl.handle.net/21.15107/rcub_rimsi_2699

Tanović M, Dimitrijević M, Milenković M, Stanić M, Jagličić Z, Spasojević I. Magnetic, redox and structural properties of Mn-O-Ca cluster, synthesized by the green microalga Chlorella sorokiniana. in Serbian Biochemical Society Twelfth Conference “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia. 2023;:125-125.
https://hdl.handle.net/21.15107/rcub_rimsi_2699 .

Tanović, Marija, Dimitrijević, Milena, Milenković, Milica, Stanić, Marina, Jagličić, Zvonko, Spasojević, Ivan, "Magnetic, redox and structural properties of Mn-O-Ca cluster, synthesized by the green microalga Chlorella sorokiniana" in Serbian Biochemical Society Twelfth Conference “Biochemistry in Biotechnology”, September 21-23, 2023, Belgrade, Serbia (2023):125-125,
https://hdl.handle.net/21.15107/rcub_rimsi_2699 .

TY  - CONF
AU  - Vitkovac, Aleksandra
AU  - Pajić, Tanja
AU  - Bukumira, Marta
AU  - Stanić, Marina
AU  - Rabasovic, Mihailo
AU  - Todorović, Nataša
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2422
AB  - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023
C3  - 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
T1  - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
EP  - 337
SP  - 334
DO  - 10.46793/ICCBI23.334V
ER  - 

@conference{
author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasovic, Mihailo and Todorović, Nataša",
year = "2023",
abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023",
journal = "2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023",
title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology",
pages = "337-334",
doi = "10.46793/ICCBI23.334V"
}

Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasovic, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023
Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023., 334-337.
https://doi.org/10.46793/ICCBI23.334V

Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasovic M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023. 2023;:334-337.
doi:10.46793/ICCBI23.334V .

Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasovic, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 (2023):334-337,
https://doi.org/10.46793/ICCBI23.334V . .

TY  - CONF
AU  - Ćukić, Aleksandra
AU  - Dimitrijević, Milena
AU  - Kovačević, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2419
AB  - Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana
EP  - 230
SP  - 230
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2419
ER  - 

@conference{
author = "Ćukić, Aleksandra and Dimitrijević, Milena and Kovačević, Snežana and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2022",
abstract = "Индустријализацијом и употребом фосилних горива, водени екосистеми су све више загађени тешким металима, укључујући и никл. Једноћелијске алге представљају значајне карике у процесу биоремедијације отпадних вода. Као стресор, никл доводи до стварања реактивних врста кисеоника (ROS) код зелених алги. Механизми за заштиту од ROS обухватају неензимске и ензимске антиоксидансе као што су глутатион, аскорбат, каротеноиди, супероксид дисмутазe (CuZnSOD, FeSOD и MnSOD), каталаза (CAT), глутатион пероксидаза и глутатион редуктаза. Циљ овог рада је био да се испитају нивои антиоксидативних ензима једноћелијске алге C. sorokiniana гајене у култури и излагане високој али нетоксичној концентрацији јона Ni(II) (1 mM) у трајању од 1 h и 24 h. Методом Western blot детектовани су нивои FeSOD, MnSOD и CAT у ћелијским екстрактима контролних и никлом третираних узорака микроалги. Тренд смањења нивоа FeSOD и MnSOD протеина уочен је након једносатног третмана никлом. Код третмана од 24 h ниво FeSOD остаје смањен, док се ниво MnSOD враћа на контролне вредности. Није било промена у нивоу CAT. Показано је да акутни стрес изазван 1 mM Ni(II), који укључује повећан ниво ROS (што је установљено одговарајућим есејима), не доводи до статистички значајних промена у нивоу испитиваних антиоксидативних ензима код C. sorokiniana. Потребно је испитати остале компоненте антиоксидативне заштите које вероватно учествују у уклањању ROS индукованих на овај начин.",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana",
pages = "230-230",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2419"
}

Ćukić, A., Dimitrijević, M., Kovačević, S., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2022). Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419

Ćukić A, Dimitrijević M, Kovačević S, Opačić M, Stanić M, Žižić M, Spasojević I. Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:230-230.
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

Ćukić, Aleksandra, Dimitrijević, Milena, Kovačević, Snežana, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Uticaj povećane koncentracije jona Ni(II) na nivo antioksidativnih enzima u jednoćelijskoj algi Chlorella sorokiniana" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):230-230,
https://hdl.handle.net/21.15107/rcub_rimsi_2419 .

TY  - CONF
AU  - Rodić, Ivanka
AU  - Lukičić, Jovana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Živić, Miroslav
AU  - Cvetic Antic, Tijana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2409
AB  - Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом
PB  - Srpsko biološko društvo, Beograd, 2022
C3  - Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
T1  - Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite
EP  - 233
SP  - 233
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2409
ER  - 

@conference{
author = "Rodić, Ivanka and Lukičić, Jovana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Živić, Miroslav and Cvetic Antic, Tijana",
year = "2022",
abstract = "Селенити могу бити веома токсични услед оксидативног стреса изазваног интеракцијама са унутарћелијским тиолима. Микробиолошка редукција селенита у елементарни селен, који је најмање токсичан облик селена, је од пресудног значаја за смањење биорасположивости овог елемента. Недавно је потврђено да гљива Phycomyces blakesleeanus има способност редукције селенита до елементарног селена. У овом раду је утврђено да селенит има инхибиторан ефекат на раст мицелије P. blakesleeanus при концентрацијама вишим од 500 μМ. Мицелије су усвајале око 30% селенита који се налазио у медијуму, а плато усвајања је достигнут на медијуму са 1 mM селенитом. Садржај глутатиона и активности ензима мерени су у мицелијама које су гајене у медијуму са 100 μM селенитом који није показивао токсичност. Већ након 3 ч третмана селенитом повећан је садржај оксидованог глутатиона код третираних мицелија у односу на контролу. Уочене су старосно зависне промене у активности ензима и концентрацији глутатиона које су се јављале код третираних мицелија и у контроли: смањење активности каталазе, смањење садржаја укупног глутатиона и повећање активности глутатион редуктазе 24 ч након преношења на свеж медијум са или без селенита. Активност каталазе, глутатион-редуктазе и -трансферазе се статистички значајно повећала у односу на контролу почев од 24 ч третмана селенитом, док је активност глутатион пероксидазе показала двофазни одговор, са повећањем активности 1 ч и 48 ч након преношења на медијум са селенитом",
publisher = "Srpsko biološko društvo, Beograd, 2022",
journal = "Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.",
title = "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite",
pages = "233-233",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2409"
}

Rodić, I., Lukičić, J., Stanić, M., Žižić, M., Zakrzewska, J., Živić, M.,& Cvetic Antic, T.. (2022). Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.
Srpsko biološko društvo, Beograd, 2022., 233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409

Rodić I, Lukičić J, Stanić M, Žižić M, Zakrzewska J, Živić M, Cvetic Antic T. Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite. in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022.. 2022;:233-233.
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

Rodić, Ivanka, Lukičić, Jovana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Živić, Miroslav, Cvetic Antic, Tijana, "Usvajanje i redukcija selenita u micelijama Phycomyces blakesleeanus: uticaj na aktivnost enzima antioksidativne zaštite" in Treći kongres biologa Srbije, osnovna i primenjena istraživanja, metodika nastave. Zlatibor, Srbija 21 – 25. 9. 2022. (2022):233-233,
https://hdl.handle.net/21.15107/rcub_rimsi_2409 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2385
AB  - Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus
EP  - 858
SP  - 858
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2385
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2022",
abstract = "Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus",
pages = "858-858",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2385"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2022). Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):858-858,
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Cvetic Antic, Tijana
AU  - Todorović, Nataša
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2113
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 96
SP  - 93
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2113
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Cvetic Antic, Tijana and Todorović, Nataša and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), with the aim of determining the flow and products of its biotransformation. Selenite is reduced to Se0 in the form selenium nanoparticles (SeNPs) and Se-2 in the form of methylated volatile selenides. Low concentrations of Se+4 in the mycelium form RSSeSR type compounds, which could be a step in the process of SeNPs formation, or incorporation of Se into metabolites such as Se-amino acids.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "96-93",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2113"
}

Žižić, M., Stanić, M., AQUILANTI, G., Cvetic Antic, T., Todorović, N., Živić, M.,& Zakrzewska, J.. (2022). SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113

Žižić M, Stanić M, AQUILANTI G, Cvetic Antic T, Todorović N, Živić M, Zakrzewska J. SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:93-96.
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Cvetic Antic, Tijana, Todorović, Nataša, Živić, Miroslav, Zakrzewska, Joanna, "SELENITE METABOLISM IN THE MYCELIUM OF THE FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):93-96,
https://hdl.handle.net/21.15107/rcub_rimsi_2113 .

TY  - CONF
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - Rodić, Ivanka
AU  - Cvetic Antic, Tijana
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Todorović, Nataša
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2114
AB  - In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.
PB  - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3  - 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1  - PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS
EP  - 92
SP  - 89
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2114
ER  - 

@conference{
author = "Žižić, Milan and Stanić, Marina and Rodić, Ivanka and Cvetic Antic, Tijana and Živić, Miroslav and Zakrzewska, Joanna and Todorović, Nataša",
year = "2022",
abstract = "In this study, mycelium of fungus Phycomyces blakesleeanus was exposed to soluble toxic form of selenium, selenite (Se+4), to examine its ability to reduce it to nanoparticles. Red coloration appeared after only a few hours of incubation with 10 mM Se+4 indicating formation of selenium nanoparticles (SeNPs). SEM-EDS confirmed pure selenium NPs with an average diameter of 57 nm, which indicates to potentially very good medical, optical and photoelectric characteristics. Raman spectroscopy showed several structural forms of SeNPs formed in the extracellular space with a monoclinic Se8 chain as the most represented, and the other observed forms were trigonal Se polymer chain, Se8 ring and Se6 chain structures.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "16th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS",
pages = "92-89",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2114"
}

Žižić, M., Stanić, M., Rodić, I., Cvetic Antic, T., Živić, M., Zakrzewska, J.,& Todorović, N.. (2022). PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 1, 89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114

Žižić M, Stanić M, Rodić I, Cvetic Antic T, Živić M, Zakrzewska J, Todorović N. PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS. in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2022;1:89-92.
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

Žižić, Milan, Stanić, Marina, Rodić, Ivanka, Cvetic Antic, Tijana, Živić, Miroslav, Zakrzewska, Joanna, Todorović, Nataša, "PRODUCTION AND CHARACTERISATION OF SELENIUM NANOPARTICLES BY MYCELIUM OF FUNGUS PHYCOMYCES BLAKESLEEANUS" in 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 1 (2022):89-92,
https://hdl.handle.net/21.15107/rcub_rimsi_2114 .

TY  - JOUR
AU  - Žižić, Milan
AU  - Stanić, Marina
AU  - AQUILANTI, Giuliana
AU  - Bajuk-Bogdanovic, Danica
AU  - Branković, Goran
AU  - Rodić, Ivanka
AU  - Živić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106
AB  - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
PB  - Springer-Verlag GmbH Berlin Heidelberg
T2  - Analytical and Bianalytical Chemistry
T1  - Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus
EP  - 6222.
IS  - 20
SP  - 6213
VL  - 414
DO  - 10.1007/s00216-022-04191-4
ER  - 

@article{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms 
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. 
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as 
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red 
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of 
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter 
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium 
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile 
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. 
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer 
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy",
publisher = "Springer-Verlag GmbH Berlin Heidelberg",
journal = "Analytical and Bianalytical Chemistry",
title = "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus",
pages = "6222.-6213",
number = "20",
volume = "414",
doi = "10.1007/s00216-022-04191-4"
}

Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry
Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222..
https://doi.org/10.1007/s00216-022-04191-4

Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222..
doi:10.1007/s00216-022-04191-4 .

Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus  Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222.,
https://doi.org/10.1007/s00216-022-04191-4 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Danilović Luković, Jelena
AU  - Milivojević, Marija
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2403
AB  - METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana
EP  - 748
SP  - 747
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2403
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Danilović Luković, Jelena and Milivojević, Marija and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "METHODS
The impact of a set of concentrations of Mn2+ and Ni2+ on growth of C. sorokiniana culture in 3N-BBM+V
medium in the early stationary phase was evaluated by changes in optical density at 750 nm and biomass
during 7 days treatment. Mucilage release was analyzed using SEM microscopy. Redox settings were analyzed
by oxidation-sensitive fluorescent probe and assays for thiols.
RESULTS
Ni was more toxic than Mn and affected culture growth at lower concentrations. Microalgal cells started
releasing mucilage polymers within 1 h of exposure to 1 mM Mn2+, whereas no mucilage was observed even
at 24 h of treatment with equimolar Ni2+. The peak of reactive oxygen species production was reached faster
for Ni2+ than Mn2+. Mn-induced drops in the concentration of reduced thiols showed a recovery after 1 h and
24 h. Ni2+-induced drop was irreversible. The observed differences between the impact of Mn and Ni may be
related to different redox and coordinative properties and to higher capacities of microalgae to sequester Mn
in relation to higher quotas than Ni that are required for normal functio",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana",
pages = "748-747",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2403"
}

Vojvodić, S., Stanić, M., Danilović Luković, J., Milivojević, M., Zechmann, B., Dimitrijević, M., Opačić, M., Pittman, J. K.,& Spasojević, I.. (2022). Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403

Vojvodić S, Stanić M, Danilović Luković J, Milivojević M, Zechmann B, Dimitrijević M, Opačić M, Pittman JK, Spasojević I. Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:747-748.
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

Vojvodić, Snežana, Stanić, Marina, Danilović Luković, Jelena, Milivojević, Marija, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Pittman, Jon K., Spasojević, Ivan, "Comparative impact of Mn2+ and Ni2+ on the microalga Chlorella sorokiniana" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):747-748,
https://hdl.handle.net/21.15107/rcub_rimsi_2403 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Dimitrijević, Milena
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2417
AB  - Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
T1  - Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2417
ER  - 

@conference{
author = "Vojvodić, Snežana and Opačić, Miloš and Stanić, Marina and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan",
year = "2022",
abstract = "Nickel is utilized by microalgae as a co-factor of urease. On the other hand, this transition metal represents an important pollutant of aquatic ecosystems. The effects of Ni(II) excess on microalgae and the mechanisms of adaptation are poorly understood. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. Pertinent to this, we analyzed redox changes in Chlorella sorokiniana culture that are induced by high levels of Ni(II). The intracellular level of reactive oxygen species (ROS) showed a rapid two-phase increase that took place prior to Ni accumulation in the cell. This was accompanied by oxidation of thiols and drastic deglutathyonilation of proteins. PAM fluorimetry showed that Ni excess induced an increase in the efficiency of photosystem II and promoted electron flow in chloroplasts, which is most likely responsible for ROS rise. In addition, a rising trend in the chlorophyll level was observed. On the other hand, the level of lipid peroxidation and activities of key antioxidative enzymes were not increased, which implies that oxidative stress is not an important player in Ni adaptation/toxicity. After prolonged exposure the efficiency of photosystem II drops, nickel is accumulated in the cells, and new redox balance is established. Our results imply that redox signalling is involved in Ni-induced metabolic activation and that key changes take place in photosynthetic machinery.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia",
title = "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2417"
}

Vojvodić, S., Opačić, M., Stanić, M., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2022). Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417

Vojvodić S, Opačić M, Stanić M, Dimitrijević M, Pittman JK, Spasojević I. Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess. in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia. 2022;:133-133.
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

Vojvodić, Snežana, Opačić, Miloš, Stanić, Marina, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "Redox component in the adaptation of the microalga Chlorella sorokiniana to Ni(II) excess" in Serbian Biochemical Society Eleventh Conference, “Amazing Biochemistry”. September 22nd and 23rd, 2022, Novi Sad, Serbia (2022):133-133,
https://hdl.handle.net/21.15107/rcub_rimsi_2417 .

TY  - CONF
AU  - Lukicic, J.
AU  - Rodic, I.
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1463
AB  - Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.
PB  - Wiley, Hoboken
C3  - Febs Open Bio
T1  - Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus
EP  - 397
SP  - 396
VL  - 11
DO  - 10.1002/2211-5463.13205
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1463
ER  - 

@conference{
author = "Lukicic, J. and Rodic, I. and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.",
publisher = "Wiley, Hoboken",
journal = "Febs Open Bio",
title = "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus",
pages = "397-396",
volume = "11",
doi = "10.1002/2211-5463.13205",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1463"
}

Lukicic, J., Rodic, I., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio
Wiley, Hoboken., 11, 396-397.
https://doi.org/10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463

Lukicic J, Rodic I, Žižić M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio. 2021;11:396-397.
doi:10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

Lukicic, J., Rodic, I., Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus" in Febs Open Bio, 11 (2021):396-397,
https://doi.org/10.1002/2211-5463.13205 .,
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Dučić, Tanja
AU  - Stanković, Dalibor
AU  - Opačić, Miloš
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2391
AB  - Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)
EP  - 174
SP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2391
ER  - 

@conference{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Dučić, Tanja and Stanković, Dalibor and Opačić, Miloš and Stanić, Marina and Žižić, Milan and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae can be implemented in the remediation of mining and other metal-rich wastewaters as pioneer species. On the other hand, metals affect aquatic ecosystems through the negative impact on microalgae which are the primary producers of O2 and biomass. Redox processes represent an important component of the mechanisms of interaction of microalgae with transition metals. We analyzed the redox changes in Chlorella sorokiniana culture that are induced by high levels of Mn(II). Mn is the key metal pollutant, with five main oxidation forms that can bind to a variety of different ligands. Mn (1 mM) induced a significant increase in the intracellular production of reactive oxygen species. The boost appears to show two phases – the first is very fast (observed after 15 min), whereas the second starts after 1 h reaching a plateau at 24 h. The concentration of reduced thiols, which represent important targets of oxidation, appears to parallel this trend. Total glutathione concentration shows a drop at 1 h and recovery at 24 h. This implicates that either a glutathionylation of proteins or a synthesis of phytochelatins - sulfur-rich short-chain peptides that sequester metals, takes place early in the response to Mn. Further, FTIR analysis showed that Mn induced a decrease of C=C levels and CH2/CH3 ratio implicating increased lipid peroxidation. Finally, Mn ions that were accumulated in the cells were extracted with nitric oxide and analyzed by cyclic voltammetry. Two redox forms were detected - Mn(II) and Mn(IV). The latter appears to prevail at higher manganese concentrations and longer periods of incubation. These results demonstrate that redox response of C. sorokiniana to high Mn levels involves at least two phases. Initially, Mn(II) enters the cells and induces pro-oxidative changes that are mitigated by glutathione-based antioxidative defense. Later on, redox homeostasis is reestablished with concomitant inactivation of Mn in the more stable redox form.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)",
pages = "174-174",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2391"
}

Vojvodić, S., Dimitrijević, M., Dučić, T., Stanković, D., Opačić, M., Stanić, M., Žižić, M.,& Spasojević, I.. (2021). Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391

Vojvodić S, Dimitrijević M, Dučić T, Stanković D, Opačić M, Stanić M, Žižić M, Spasojević I. Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II). in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:174-174.
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

Vojvodić, Snežana, Dimitrijević, Milena, Dučić, Tanja, Stanković, Dalibor, Opačić, Miloš, Stanić, Marina, Žižić, Milan, Spasojević, Ivan, "Redox changes in microalga Chlorella sorokiniana exposed to high concentrations of Mn(II)" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):174-174,
https://hdl.handle.net/21.15107/rcub_rimsi_2391 .

TY  - CONF
AU  - Žižić, Milan
AU  - Macura, Melanija
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1470
AB  - Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.204
ER  - 

@conference{
author = "Žižić, Milan and Macura, Melanija and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.204"
}

Žižić, M., Macura, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://doi.org/10.1016/j.freeradbiomed.2021.08.204

Žižić M, Macura M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine. 2021;177.
doi:10.1016/j.freeradbiomed.2021.08.204 .

Žižić, Milan, Macura, Melanija, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR" in Free Radical Biology and Medicine, 177 (2021),
https://doi.org/10.1016/j.freeradbiomed.2021.08.204 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2420
AB  - Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.
PB  - Elsevier
PB  - Society for Free Radical Research Europe (SFRR-E)
PB  - Ministry of Education, Science and Technological Development (Republic of Serbia)
PB  - Oxygen Club of California
C3  - Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
T1  - Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana
EP  - 145
SP  - 145
DO  - 10.1016/j.freeradbiomed.2021.08.148
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Danilović Luković, Jelena and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2021",
abstract = "Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.",
publisher = "Elsevier, Society for Free Radical Research Europe (SFRR-E), Ministry of Education, Science and Technological Development (Republic of Serbia), Oxygen Club of California",
journal = "Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts",
title = "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana",
pages = "145-145",
doi = "10.1016/j.freeradbiomed.2021.08.148"
}

Vojvodić, S., Stanić, M., Zechmann, B., Dimitrijević, M., Opačić, M., Danilović Luković, J., Morina, A., Pittman, J. K.,& Spasojević, I.. (2021). Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
Elsevier., 145-145.
https://doi.org/10.1016/j.freeradbiomed.2021.08.148

Vojvodić S, Stanić M, Zechmann B, Dimitrijević M, Opačić M, Danilović Luković J, Morina A, Pittman JK, Spasojević I. Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts. 2021;:145-145.
doi:10.1016/j.freeradbiomed.2021.08.148 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Danilović Luković, Jelena, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana" in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts (2021):145-145,
https://doi.org/10.1016/j.freeradbiomed.2021.08.148 . .

TY  - CONF
AU  - Dimitrijević, Milena
AU  - Vojvodić, Snežana
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Milić, Jelena
AU  - Stanić, Marina
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2392
AB  - Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.
PB  - Faculty of Chemistry, Serbian Biochemical Society
C3  - Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
T1  - The effect of increased nickel concentrations on Chlorella sorokiniana culture
EP  - 58
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2392
ER  - 

@conference{
author = "Dimitrijević, Milena and Vojvodić, Snežana and Opačić, Miloš and Danilović Luković, Jelena and Milić, Jelena and Stanić, Marina and Spasojević, Ivan",
year = "2021",
abstract = "Microalgae show significant biotechnological potential for remediation of wastewaters polluted with metals, including nickel. Ni enters aquatic ecosystems from fuel combustion, smelting, mining, and electroplating endeavors, and represents a particularly important problem for the access to safe drinking water. It is noteworthy that microalgae utilize Ni as a co-factor of urease. Herein we analyzed the impact of high levels of Ni(II) on freshwater microalga Chlorella sorokiniana in the stationary phase of culture growth. In the concentration range 0.5 to 30 mM, Ni induced a drop in cell density and biomass after 7 days of incubation, whereas significant negative impact was present as early as 2 days for 30 mM. High concentrations also induced a decrease in the level of chlorophylls (a and b) in biomass. In contrast, microalgae that were exposed to moderate stress (1 and 2 mM of Ni) appear to increase photosynthetic activity, indicating the activation of some adaptive mechanism. Scanning electron microscopy showed that C. sorokiniana does not release significant amounts of mucilage polymers in response to Ni, in contrast to the response to some other metals 3. Further, Ni at 1 mM induced an increase in the intracellular production of reactive oxygen species, which appears to reach a plateau after 1 h. The concentration of free thiol groups showed a gradual drop during 24 h of incubation with the same Ni concentration. On the other hand, the decrease in the concentration of reduced glutathione that was observed after 1 h was reversible for 1 mM Ni at 24 h. Ni at 5 mM had a more lasting impact on glutathione. It is important to stress out that the concentrations of reduced glutathione and total glutathione showed very similar trends. This implies that free thiols and reduced glutathione may be ‘lost’ due to glutathionylation of proteins and synthesis of phytochelatins. Our results demonstrate that the response of C. sorokiniana to high Ni levels involves multiple components that may be different than for other metals. Redox regulation and protection of thiol (switches) may represent a crossroad between adaptation and death.",
publisher = "Faculty of Chemistry, Serbian Biochemical Society",
journal = "Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia",
title = "The effect of increased nickel concentrations on Chlorella sorokiniana culture",
pages = "58-57",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2392"
}

Dimitrijević, M., Vojvodić, S., Opačić, M., Danilović Luković, J., Milić, J., Stanić, M.,& Spasojević, I.. (2021). The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia
Faculty of Chemistry, Serbian Biochemical Society., 57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392

Dimitrijević M, Vojvodić S, Opačić M, Danilović Luković J, Milić J, Stanić M, Spasojević I. The effect of increased nickel concentrations on Chlorella sorokiniana culture. in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia. 2021;:57-58.
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

Dimitrijević, Milena, Vojvodić, Snežana, Opačić, Miloš, Danilović Luković, Jelena, Milić, Jelena, Stanić, Marina, Spasojević, Ivan, "The effect of increased nickel concentrations on Chlorella sorokiniana culture" in Serbian Biochemical Society Tenth Conference, “Biochemical Insights into Molecular Mechanisms”. 24.09.2021. Kragujevac, Serbia (2021):57-58,
https://hdl.handle.net/21.15107/rcub_rimsi_2392 .

TY  - JOUR
AU  - Živanović, Branka  D.
AU  - Danilović Luković, Jelena
AU  - Korac, Aleksandra
AU  - Stanić, Marina
AU  - Spasić, Slađana
AU  - Galland, Paul
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1399
AB  - The growing zone (GZ) of the unicellular coenocytic sporangiophore of Phycomyces blakesleeanus represents the site of stimulus reception (light, gravity, gas) and stimulus response, i.e., local modulations of the elongation growth, which may result, in dependence of the stimulus direction, in tropic bending. Until now, evidence for a possible participation of the columella in sensory reception is absent. We confirm with light microscopy earlier studies that show that the GZ and the columella are not separated by a membrane or cell wall, but rather form a spatial continuum that allows free exchange of cytoplasm and organelle transport. Evidence is presented that the columella is responsive to external stimuli. Columellae, from which spores and sporangial cell wall had been removed, respond to exogenous auxin with a local depolarization of the membrane potential and an increased growth rate of the GZ. In contrast, auxin applied to the GZ causes a decrease of the growth rate irrespective of the presence or absence of sporangia. The response pattern is specific and relevant for the sensory reception of Phycomyces, because the light-insensitive mutant C148carAmadC, which lacks the RAS-GAP protein MADC, displays abnormal IAA sensitivity and membrane depolarization. We argue that the traditional concept of the GZ as the only stimulus-sensitive zone should be abandoned in favor of a model in which GZ and columella operate as a single entity capable to orchestrate a multitude of stimulus inputs, including auxin, to modulate the membrane potential and elongation growth of the GZ.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential
DO  - 10.1007/s00709-021-01709-y
ER  - 

@article{
author = "Živanović, Branka  D. and Danilović Luković, Jelena and Korac, Aleksandra and Stanić, Marina and Spasić, Slađana and Galland, Paul",
year = "2021",
abstract = "The growing zone (GZ) of the unicellular coenocytic sporangiophore of Phycomyces blakesleeanus represents the site of stimulus reception (light, gravity, gas) and stimulus response, i.e., local modulations of the elongation growth, which may result, in dependence of the stimulus direction, in tropic bending. Until now, evidence for a possible participation of the columella in sensory reception is absent. We confirm with light microscopy earlier studies that show that the GZ and the columella are not separated by a membrane or cell wall, but rather form a spatial continuum that allows free exchange of cytoplasm and organelle transport. Evidence is presented that the columella is responsive to external stimuli. Columellae, from which spores and sporangial cell wall had been removed, respond to exogenous auxin with a local depolarization of the membrane potential and an increased growth rate of the GZ. In contrast, auxin applied to the GZ causes a decrease of the growth rate irrespective of the presence or absence of sporangia. The response pattern is specific and relevant for the sensory reception of Phycomyces, because the light-insensitive mutant C148carAmadC, which lacks the RAS-GAP protein MADC, displays abnormal IAA sensitivity and membrane depolarization. We argue that the traditional concept of the GZ as the only stimulus-sensitive zone should be abandoned in favor of a model in which GZ and columella operate as a single entity capable to orchestrate a multitude of stimulus inputs, including auxin, to modulate the membrane potential and elongation growth of the GZ.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential",
doi = "10.1007/s00709-021-01709-y"
}

Živanović, Branka  D., Danilović Luković, J., Korac, A., Stanić, M., Spasić, S.,& Galland, P.. (2021). Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential. in Protoplasma
Springer Wien, Wien..
https://doi.org/10.1007/s00709-021-01709-y

Živanović, Branka  D., Danilović Luković J, Korac A, Stanić M, Spasić S, Galland P. Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential. in Protoplasma. 2021;.
doi:10.1007/s00709-021-01709-y .

Živanović, Branka  D., Danilović Luković, Jelena, Korac, Aleksandra, Stanić, Marina, Spasić, Slađana, Galland, Paul, "Signal transduction in Phycomyces sporangiophores: columella as a novel sensory organelle mediating auxin-modulated growth rate and membrane potential" in Protoplasma (2021),
https://doi.org/10.1007/s00709-021-01709-y . .

TY  - CONF
AU  - Kovačević, Aleksander
AU  - Pajić, Tanja
AU  - Pavlović, Danica
AU  - Stanić, Marina
AU  - Lekić, Marina
AU  - Nikolić, Stanko
AU  - Jelenković, Branislav
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2411
AB  - In the experiments, the 532 nm CW laser beam is directed to the glass cuvette that is filled either with the medium or with algae suspended in the medium. We have monitored the laser beam diameter at the entrance and exit of the cuvette, and its axial profile through entire cell length. The concentration has been determined by optical microscopy and optical density and has been varied between 10^6 and 10^8 cm-3. The concentration of the algae and the laser beam power affect the beam radius. Our preliminary results have shown the effect of light self-trapping, i.e., the decrease of laser diameter when the algae concentration exceeds 10^6 cm-3 while laser power is above 1 W. The difference of the refractive indexes of the algae and the medium can induce optical trapping of algae, which subsequently changes the concentration of the algae within the laser beam. This in turn can explain different behavior of the beam in the medium with and without algae.
PB  - Institute of Physics Belgrade, Pregrevica 118, 11080 Belgrade, Serbia
C3  - VIII International School and Conference on Photonics PHOTONICA2021 & HEMMAGINERO workshop, 23 - 27 August 2021
T1  - Narrowing of laser beam propagating through biological suspension
EP  - 75
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2411
ER  - 

@conference{
author = "Kovačević, Aleksander and Pajić, Tanja and Pavlović, Danica and Stanić, Marina and Lekić, Marina and Nikolić, Stanko and Jelenković, Branislav",
year = "2021",
abstract = "In the experiments, the 532 nm CW laser beam is directed to the glass cuvette that is filled either with the medium or with algae suspended in the medium. We have monitored the laser beam diameter at the entrance and exit of the cuvette, and its axial profile through entire cell length. The concentration has been determined by optical microscopy and optical density and has been varied between 10^6 and 10^8 cm-3. The concentration of the algae and the laser beam power affect the beam radius. Our preliminary results have shown the effect of light self-trapping, i.e., the decrease of laser diameter when the algae concentration exceeds 10^6 cm-3 while laser power is above 1 W. The difference of the refractive indexes of the algae and the medium can induce optical trapping of algae, which subsequently changes the concentration of the algae within the laser beam. This in turn can explain different behavior of the beam in the medium with and without algae.",
publisher = "Institute of Physics Belgrade, Pregrevica 118, 11080 Belgrade, Serbia",
journal = "VIII International School and Conference on Photonics PHOTONICA2021 & HEMMAGINERO workshop, 23 - 27 August 2021",
title = "Narrowing of laser beam propagating through biological suspension",
pages = "75-75",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2411"
}

Kovačević, A., Pajić, T., Pavlović, D., Stanić, M., Lekić, M., Nikolić, S.,& Jelenković, B.. (2021). Narrowing of laser beam propagating through biological suspension. in VIII International School and Conference on Photonics PHOTONICA2021 & HEMMAGINERO workshop, 23 - 27 August 2021
Institute of Physics Belgrade, Pregrevica 118, 11080 Belgrade, Serbia., 75-75.
https://hdl.handle.net/21.15107/rcub_rimsi_2411

Kovačević A, Pajić T, Pavlović D, Stanić M, Lekić M, Nikolić S, Jelenković B. Narrowing of laser beam propagating through biological suspension. in VIII International School and Conference on Photonics PHOTONICA2021 & HEMMAGINERO workshop, 23 - 27 August 2021. 2021;:75-75.
https://hdl.handle.net/21.15107/rcub_rimsi_2411 .

Kovačević, Aleksander, Pajić, Tanja, Pavlović, Danica, Stanić, Marina, Lekić, Marina, Nikolić, Stanko, Jelenković, Branislav, "Narrowing of laser beam propagating through biological suspension" in VIII International School and Conference on Photonics PHOTONICA2021 & HEMMAGINERO workshop, 23 - 27 August 2021 (2021):75-75,
https://hdl.handle.net/21.15107/rcub_rimsi_2411 .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Danilović Luković, Jelena
AU  - Zechmann, Bernd
AU  - Jevtović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Lizzul, Alessandro Marco
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1371
AB  - The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana
VL  - 260
DO  - 10.1016/j.chemosphere.2020.127553
ER  - 

@article{
author = "Vojvodić, Snežana and Danilović Luković, Jelena and Zechmann, Bernd and Jevtović, Mima and Bogdanović Pristov, Jelena and Stanić, Marina and Lizzul, Alessandro Marco and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "The impact of ionizing radiation on microorganisms such as microalgae is a topic of increasing importance for understanding the dynamics of aquatic ecosystems in response to environmental radiation, and for the development of efficient approaches for bioremediation of mining and nuclear power plants wastewaters. Currently, nothing is known about the effects of ionizing radiation on the microalgal cell wall, which represents the first line of defence against chemical and physical environmental stresses. Using various microscopy, spectroscopy and biochemical techniques we show that the unicellular alga Chlorella sorokiniana elicits a fast response to ionizing radiation. Within one day after irradiation with doses of 1-5 Gy, the fibrilar layer of the cell wall became thicker, the fraction of uronic acids was higher, and the capacity to remove the main reactive product of water radiolysis increased. In addition, the isolated cell wall fraction showed significant binding capacity for Cu2+, Mn2+, and Cr3+. The irradiation further increased the binding capacity for Cu2+, which appears to be mainly bound to glucosamine moieties within a chitosan-like polymer in the outer rigid layer of the wall. These results imply that the cell wall represents a dynamic structure that is involved in the protective response of microalgae to ionizing radiation. It appears that microalgae may exhibit a significant control of metal mobility in aquatic ecosystems via biosorption by the cell wall matrix.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana",
volume = "260",
doi = "10.1016/j.chemosphere.2020.127553"
}

Vojvodić, S., Danilović Luković, J., Zechmann, B., Jevtović, M., Bogdanović Pristov, J., Stanić, M., Lizzul, A. M., Pittman, J. K.,& Spasojević, I.. (2020). The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 260.
https://doi.org/10.1016/j.chemosphere.2020.127553

Vojvodić S, Danilović Luković J, Zechmann B, Jevtović M, Bogdanović Pristov J, Stanić M, Lizzul AM, Pittman JK, Spasojević I. The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana. in Chemosphere. 2020;260.
doi:10.1016/j.chemosphere.2020.127553 .

Vojvodić, Snežana, Danilović Luković, Jelena, Zechmann, Bernd, Jevtović, Mima, Bogdanović Pristov, Jelena, Stanić, Marina, Lizzul, Alessandro Marco, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing radiation on the structure and antioxidative and metal-binding capacity of the cell wall of microalga Chlorella sorokiniana" in Chemosphere, 260 (2020),
https://doi.org/10.1016/j.chemosphere.2020.127553 . .