TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2385
AB  - Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.
C3  - FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.
T1  - Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus
EP  - 858
SP  - 858
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2385
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2022",
abstract = "Phycomyces blakesleeanus is a strict aerobic filamentous fungus often used as a model system in studies of physiology, genetics, environmental sensing, and metabolism. As all other aerobic organisms, this fungus faces the toxic effects of oxygen-reactive species, but data about its antioxidative defense systems are scarce. The aim of this research was to examine the activities of three antioxidant enzymes during different phases of growth. The fungus was grown in two ways, in Petri dishes, and on a shaker in Erlenmeyer flasks. The activities of superoxide dismutase
(SOD) and peroxidase (POD) were determined spectrophotometrically, while the activity of catalase (CAT) was determined polarographically with a Clark-type oxygen electrode. The highest activities of SOD were noticed in mycelia grown in Erlenmeyer flasks in the stationary phase of growth. In mycelia grown in Petri dishes, the highest activities of POD (0,014U/mg protein) and CAT (20,63 U/mg protein) were noticed in the early exponential phase. The activities of these two enzymes decreased with mycelial growth. In mycelia grown in Erlenmeyer flasks, POD and CAT
showed similar behavior, but differences in activities between exponential and stationary phases were smaller and the highest activities were noticed in the mid-exponential to stationary phase of growth (0,011U/mg protein for POD and 17,21U/mg protein for CAT). High activities of these two enzymes indicated increased production of H2O2 and pointed out the importance of this phase for mycelia grown in this way.",
journal = "FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.",
title = "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus",
pages = "858-858",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2385"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2022). Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia., 858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus. in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia.. 2022;:858-858.
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Activities of antioxidant enzymes in mycelium of fungus Phycomyces blakesleeanus" in FEMS Conference on Microbiology (in association with Serbian Society of Microbiology), Belgrade, Serbia. (2022):858-858,
https://hdl.handle.net/21.15107/rcub_rimsi_2385 .

TY  - CONF
AU  - Lukičić, Jovana
AU  - Rodić, Ivanka
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić Antič, Tijana
AU  - Živić, Miroslav
AU  - Stanić, Marina
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2205
AB  - Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke
PB  - Srpsko biološko društvo, Beograd
C3  - DRUGI KONGRES BIOLOGA SRBIJE
T1  - Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus
EP  - 33
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2205
ER  - 

@conference{
author = "Lukičić, Jovana and Rodić, Ivanka and Žižić, Milan and Zakrzewska, Joanna and Cvetić Antič, Tijana and Živić, Miroslav and Stanić, Marina",
year = "2018",
abstract = "Vanadijum je prelazni metal, koji se može naći u brojnim oksidacionim stanjima, pri čemu su najznačajnija +4 (vanadil) i +5 (vanadat). S obzirom da gljive apsorbuju vanadijum, one predstavljaju glavni put njegovog ulaska u ekosistem.1 Vanadat je izučavan kao potencijalni antidijabetski i antikancerogeni agens, ali zbog svoje komplikovane hemije se ne koristi u terapijama. Mnogi efekti vanadijuma na ćeliju nisu poznati, a među njima je i efekat na sisteme antioksidativne zaštite.2 U ovom istraživanju je ispitivana aktivnost superoksid dismutaza (SOD), peroksidaza (Prx) i katalaza (CAT) tokom različitih faza razvoja gljive Phycomyces blakesleeanus, a kao odgovor na tretman micelijuma vanadatom. Efekat je praćen kod gljiva starosti 20 h, 36 h i 56 h, koje su izlagane 10 mM vanadatu tokom 1 h, 3 h ili 5 h. U eksponencijalnoj fazi razvoja (20 h) zabeležen je porast aktivnosti Prx i to 45±7% nakon 1 h, odnosno 19±7% nakon 5 h tretmana. Na prelasku iz eksponencijalne u stacionarnu fazu razvoja (36 h) 10 mM vanadat nije doveo do promene aktivnosti Prx u tretiranim uzorcima, dok je u stacionarnoj fazi razvoja (56 h) ponovo zabeležen porast aktivnosti Prx i to 22±4% nakon 1 h, odnosno 31±19% nakon 3 h tretmana. Porast aktivnosti SOD je zabeležen samo u stacionarnoj fazi razvoja, nakon 1 h tretmana i iznosi 26±2%. Nije uočena jasna promena aktivnosti CAT tretiranih u odnosu na kontrolne uzorke",
publisher = "Srpsko biološko društvo, Beograd",
journal = "DRUGI KONGRES BIOLOGA SRBIJE",
title = "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2205"
}

Lukičić, J., Rodić, I., Žižić, M., Zakrzewska, J., Cvetić Antič, T., Živić, M.,& Stanić, M.. (2018). Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE
Srpsko biološko društvo, Beograd., 33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205

Lukičić J, Rodić I, Žižić M, Zakrzewska J, Cvetić Antič T, Živić M, Stanić M. Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus. in DRUGI KONGRES BIOLOGA SRBIJE. 2018;:33-33.
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .

Lukičić, Jovana, Rodić, Ivanka, Žižić, Milan, Zakrzewska, Joanna, Cvetić Antič, Tijana, Živić, Miroslav, Stanić, Marina, "Efekti vanadata na aktivnost antioksidativnih enzima tokom razvića gljive Phycomyces blakesleeanus" in DRUGI KONGRES BIOLOGA SRBIJE (2018):33-33,
https://hdl.handle.net/21.15107/rcub_rimsi_2205 .