TY  - CONF
AU  - Lukicic, J.
AU  - Rodic, I.
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1463
AB  - Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.
PB  - Wiley, Hoboken
C3  - Febs Open Bio
T1  - Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus
EP  - 397
SP  - 396
VL  - 11
DO  - 10.1002/2211-5463.13205
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1463
ER  - 

@conference{
author = "Lukicic, J. and Rodic, I. and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.",
publisher = "Wiley, Hoboken",
journal = "Febs Open Bio",
title = "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus",
pages = "397-396",
volume = "11",
doi = "10.1002/2211-5463.13205",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1463"
}

Lukicic, J., Rodic, I., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio
Wiley, Hoboken., 11, 396-397.
https://doi.org/10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463

Lukicic J, Rodic I, Žižić M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio. 2021;11:396-397.
doi:10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

Lukicic, J., Rodic, I., Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus" in Febs Open Bio, 11 (2021):396-397,
https://doi.org/10.1002/2211-5463.13205 .,
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

TY  - CONF
AU  - Žižić, Milan
AU  - Macura, Melanija
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1470
AB  - Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.204
ER  - 

@conference{
author = "Žižić, Milan and Macura, Melanija and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.204"
}

Žižić, M., Macura, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://doi.org/10.1016/j.freeradbiomed.2021.08.204

Žižić M, Macura M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine. 2021;177.
doi:10.1016/j.freeradbiomed.2021.08.204 .

Žižić, Milan, Macura, Melanija, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR" in Free Radical Biology and Medicine, 177 (2021),
https://doi.org/10.1016/j.freeradbiomed.2021.08.204 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić-Marković, Jasmina
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav
AU  - Cvetic, Antic, Tijana N.
AU  - Žižić, Milan
AU  - Todorović, Nataša
AU  - Vukic, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1457
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić-Marković, Jasmina and Filipović, Nenad D. and Zivić, Miroslav and Cvetic, Antic, Tijana N. and Žižić, Milan and Todorović, Nataša and Vukic, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić-Marković, J., Filipović, N. D., Zivić, M., Cvetic, A. T. N., Žižić, M., Todorović, N., Vukic, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić-Marković J, Filipović ND, Zivić M, Cvetic ATN, Žižić M, Todorović N, Vukic M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić-Marković, Jasmina, Filipović, Nenad D., Zivić, Miroslav, Cvetic, Antic, Tijana N., Žižić, Milan, Todorović, Nataša, Vukic, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Cvetić-Antić, Tijana
AU  - Janosević, Dusica
AU  - Maksimović, Vuk
AU  - Zivić, Miroslav
AU  - Budimir, Snežana M
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
ER  - 

@article{
author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086"
}

Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 244.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244.
doi:10.1016/j.jplph.2019.153086 .

Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020),
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Karaman, Maja
AU  - Atlagic, Kristina
AU  - Novaković, Aleksandra
AU  - Sibul, Filip
AU  - Zivić, Miroslav
AU  - Stevanović, Katarina
AU  - Pejin, Boris
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1198
AB  - Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.
PB  - MDPI, Basel
T2  - Antioxidants
T1  - Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms
IS  - 10
VL  - 8
DO  - 10.3390/antiox8100480
ER  - 

@article{
author = "Karaman, Maja and Atlagic, Kristina and Novaković, Aleksandra and Sibul, Filip and Zivić, Miroslav and Stevanović, Katarina and Pejin, Boris",
year = "2019",
abstract = "Compared to plants, nowadays mushrooms attract more attention as functional foods, due to a number of advantages in manipulating them. This study aimed to screen the chemical composition (fatty acids and phenolics) and antioxidant potential (OH center dot, 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) and ferric reducing ability of plasma (FRAP)) of two edible mushrooms, Coprinus comatus and Coprinellus truncorum, collected from nature and submerged cultivation. Partial least square regression analysis has pointed out the importance of some fatty acids-more precisely, unsaturated fatty acids (UFAs) followed by fatty acids possessing both short (C6:0 and C8:0) and long (C23:0 and C24:0) saturated chains-and phenolic compounds (such as protocatechuic acid, daidzein, p-hydroxybenzoic acid, genistein and vanillic acid) for promising anti-OH center dot, FRAP and anti-DPPH center dot activities, respectively. However, other fatty acids (C16:0, C18:0 and C18:3n3) along with the flavonol isorhamnetin are actually suspected to negatively affect (by acting pro-oxidative) the aforementioned parameters, respectively. Taken together, design of new food supplements targeting oxidative stress might be predominantly based on the various UFAs combinations (C18:2n6, C20:1, C20:2, C20:4n6, C22:2, C22:1n9, etc.), particularly if OH center dot is suspected to play an important role.",
publisher = "MDPI, Basel",
journal = "Antioxidants",
title = "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms",
number = "10",
volume = "8",
doi = "10.3390/antiox8100480"
}

Karaman, M., Atlagic, K., Novaković, A., Sibul, F., Zivić, M., Stevanović, K.,& Pejin, B.. (2019). Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants
MDPI, Basel., 8(10).
https://doi.org/10.3390/antiox8100480

Karaman M, Atlagic K, Novaković A, Sibul F, Zivić M, Stevanović K, Pejin B. Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms. in Antioxidants. 2019;8(10).
doi:10.3390/antiox8100480 .

Karaman, Maja, Atlagic, Kristina, Novaković, Aleksandra, Sibul, Filip, Zivić, Miroslav, Stevanović, Katarina, Pejin, Boris, "Fatty Acids Predominantly Affect Anti-Hydroxyl Radical Activity and FRAP Value: The Case Study of Two Edible Mushrooms" in Antioxidants, 8, no. 10 (2019),
https://doi.org/10.3390/antiox8100480 . .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica D
AU  - Baščarević, Vladimir
AU  - Zivić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1115
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Antioxidants & Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
EP  - 1575
IS  - 17
SP  - 1570
VL  - 28
DO  - 10.1089/ars.2017.7433
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica D and Baščarević, Vladimir and Zivić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Antioxidants & Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
pages = "1575-1570",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433"
}

Opačić, M., Stević, Z. D., Baščarević, V., Zivić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling
Mary Ann Liebert, Inc, New Rochelle., 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević ZD, Baščarević V, Zivić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica D, Baščarević, Vladimir, Zivić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants & Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajic, Tanja
AU  - Ciric, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Todorović, Nataša
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1082
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
EP  - 372
IS  - 3
SP  - 364
VL  - 163
DO  - 10.1099/mic.0.000429
ER  - 

@article{
author = "Stanić, Marina and Krizak, Strahinja and Jovanović, Mirna and Pajic, Tanja and Ciric, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Todorović, Nataša and Zivić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
pages = "372-364",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429"
}

Stanić, M., Krizak, S., Jovanović, M., Pajic, T., Ciric, A., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Todorović, N.,& Zivić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm
Microbiology Soc, London., 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Krizak S, Jovanović M, Pajic T, Ciric A, Žižić M, Zakrzewska J, Cvetić-Antić T, Todorović N, Zivić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marina, Krizak, Strahinja, Jovanović, Mirna, Pajic, Tanja, Ciric, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Todorović, Nataša, Zivić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology-Sgm, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Hadzibrahimović, Mirzeta
AU  - Sužnjević, Desanka
AU  - Pastor, Ferenc
AU  - Cvetić-Antić, Tijana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1064
AB  - The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?
EP  - 373
IS  - 3
SP  - 365
VL  - 110
DO  - 10.1007/s10482-016-0808-0
ER  - 

@article{
author = "Hadzibrahimović, Mirzeta and Sužnjević, Desanka and Pastor, Ferenc and Cvetić-Antić, Tijana and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav",
year = "2017",
abstract = "The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?",
pages = "373-365",
number = "3",
volume = "110",
doi = "10.1007/s10482-016-0808-0"
}

Hadzibrahimović, M., Sužnjević, D., Pastor, F., Cvetić-Antić, T., Žižić, M., Zakrzewska, J.,& Zivić, M.. (2017). The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 110(3), 365-373.
https://doi.org/10.1007/s10482-016-0808-0

Hadzibrahimović M, Sužnjević D, Pastor F, Cvetić-Antić T, Žižić M, Zakrzewska J, Zivić M. The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2017;110(3):365-373.
doi:10.1007/s10482-016-0808-0 .

Hadzibrahimović, Mirzeta, Sužnjević, Desanka, Pastor, Ferenc, Cvetić-Antić, Tijana, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 110, no. 3 (2017):365-373,
https://doi.org/10.1007/s10482-016-0808-0 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Miladinović, Zoran P.
AU  - Stanić, Marina
AU  - Hadzibrahimović, Mirzeta
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/986
AB  - V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium
EP  - 528
IS  - 6
SP  - 521
VL  - 167
DO  - 10.1016/j.resmic.2016.04.012
ER  - 

@article{
author = "Žižić, Milan and Miladinović, Zoran P. and Stanić, Marina and Hadzibrahimović, Mirzeta and Zivić, Miroslav and Zakrzewska, Joanna",
year = "2016",
abstract = "V-51 NMR spectroscopy was used for detection and identification of cell-associated vanadate (V5+) species after exposure of Phycomyces blakesleeanus mycelium, in exponential phase of growth, to sodium orthovanadate. Complete disappearance of monomer and dimer signals and decreased intensity of the tetramer signal were observed about 40 min after treatment. Simultaneously, a signal at -532 ppm, with increasing intensity, was detected in spectra. The time-dependent rise in this signal was connected to a decrease in the extracellular monomer signal, indicating its transport into the cell. The signal at -532 ppm did not belong to any known simple oxido-vanadate species, nor to a complex with any of the components of experimental medium. This signal was the only one present in spectrum of the mycelium washed 35 min after treatment, and the only one observed in mycelium cultivated on vanadate-contained medium. Therefore, its appearance can be attributed to intracellular complexation, and may represent an important detoxification mechanism of the cell exposed to a physiologically relevant concentration of vanadate. Experiments (V-51 NMR and polarography) performed with Cd-pretreated mycelium (inhibitor of an enzyme responsible for V5+ reduction) and ferricyanide-preincubated mycelium excluded the possibility of V5+ tetramer's entry into the cell.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium",
pages = "528-521",
number = "6",
volume = "167",
doi = "10.1016/j.resmic.2016.04.012"
}

Žižić, M., Miladinović, Z. P., Stanić, M., Hadzibrahimović, M., Zivić, M.,& Zakrzewska, J.. (2016). V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 167(6), 521-528.
https://doi.org/10.1016/j.resmic.2016.04.012

Žižić M, Miladinović ZP, Stanić M, Hadzibrahimović M, Zivić M, Zakrzewska J. V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium. in Research in Microbiology. 2016;167(6):521-528.
doi:10.1016/j.resmic.2016.04.012 .

Žižić, Milan, Miladinović, Zoran P., Stanić, Marina, Hadzibrahimović, Mirzeta, Zivić, Miroslav, Zakrzewska, Joanna, "V-51 NMR investigation of cell-associated vanadate species in Phycomyces blakesleeanus mycelium" in Research in Microbiology, 167, no. 6 (2016):521-528,
https://doi.org/10.1016/j.resmic.2016.04.012 . .

TY  - JOUR
AU  - Krizak, Strahinja
AU  - Nikolić, Ljiljana
AU  - Stanić, Marina
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
AU  - Todorović, Nataša
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/869
AB  - We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores
EP  - 173
IS  - 3
SP  - 162
VL  - 166
DO  - 10.1016/j.resmic.2015.02.004
ER  - 

@article{
author = "Krizak, Strahinja and Nikolić, Ljiljana and Stanić, Marina and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav and Todorović, Nataša",
year = "2015",
abstract = "We describe here whole-cell currents of droplets prepared from the apical region of growing Phycomyces blakesleeanus sporangiophores. Whole-cell current recordings revealed the osmotically activated, outwardly rectifying, fast inactivating instantaneous current (ORIC) with biophysical properties closely resembling volume-regulated anionic current (VRAC). ORIC is activated under conditions of osmotically induced swelling and shows strong selectivity for anions over cations. In addition, ORIC shows voltage and time-dependent inactivation at positive potentials and recovery from inactivation at negative potentials. ORIC is blocked by anthracene-9-carboxylic acid, an anion channel blocker, in a voltage-dependent manner. This is the first report of the presence of VRAC-like current in an organism outside the chordate lineage.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores",
pages = "173-162",
number = "3",
volume = "166",
doi = "10.1016/j.resmic.2015.02.004"
}

Krizak, S., Nikolić, L., Stanić, M., Žižić, M., Zakrzewska, J., Zivić, M.,& Todorović, N.. (2015). Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology
Elsevier, Amsterdam., 166(3), 162-173.
https://doi.org/10.1016/j.resmic.2015.02.004

Krizak S, Nikolić L, Stanić M, Žižić M, Zakrzewska J, Zivić M, Todorović N. Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores. in Research in Microbiology. 2015;166(3):162-173.
doi:10.1016/j.resmic.2015.02.004 .

Krizak, Strahinja, Nikolić, Ljiljana, Stanić, Marina, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, Todorović, Nataša, "Osmotic swelling activates a novel anionic current with VRAC-like properties in a cytoplasmic droplet membrane from Phycomyces blakesleeanus sporangiophores" in Research in Microbiology, 166, no. 3 (2015):162-173,
https://doi.org/10.1016/j.resmic.2015.02.004 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta
AU  - Pajdic, Aleksandra
AU  - Krizak, Strahinja
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/809
AB  - The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus
EP  - 857
IS  - 2
SP  - 847
VL  - 66
DO  - 10.2298/ABS1402847S
ER  - 

@article{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta and Pajdic, Aleksandra and Krizak, Strahinja and Žižić, Milan and Zakrzewska, Joanna",
year = "2014",
abstract = "The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus",
pages = "857-847",
number = "2",
volume = "66",
doi = "10.2298/ABS1402847S"
}

Stanić, M., Zivić, M., Hadzibrahimović, M., Pajdic, A., Krizak, S., Žižić, M.,& Zakrzewska, J.. (2014). Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 847-857.
https://doi.org/10.2298/ABS1402847S

Stanić M, Zivić M, Hadzibrahimović M, Pajdic A, Krizak S, Žižić M, Zakrzewska J. Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences. 2014;66(2):847-857.
doi:10.2298/ABS1402847S .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta, Pajdic, Aleksandra, Krizak, Strahinja, Žižić, Milan, Zakrzewska, Joanna, "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus" in Archives of Biological Sciences, 66, no. 2 (2014):847-857,
https://doi.org/10.2298/ABS1402847S . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Maksimović, Vuk
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/750
AB  - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
IS  - 7
VL  - 9
DO  - 10.1371/journal.pone.0102849
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2014",
abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus",
number = "7",
volume = "9",
doi = "10.1371/journal.pone.0102849"
}

Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One
Public Library Science, San Francisco., 9(7).
https://doi.org/10.1371/journal.pone.0102849

Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7).
doi:10.1371/journal.pone.0102849 .

Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014),
https://doi.org/10.1371/journal.pone.0102849 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zakrzewska, Joanna
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Marković, Zoran S
AU  - Vučinić, Željko
AU  - Zivić, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/658
AB  - Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism
EP  - 778
IS  - 7
SP  - 770
VL  - 164
DO  - 10.1016/j.resmic.2013.03.002
ER  - 

@article{
author = "Stanić, Marina and Zakrzewska, Joanna and Hadzibrahimović, Mirzeta R and Žižić, Milan and Marković, Zoran S and Vučinić, Željko and Zivić, Miroslav",
year = "2013",
abstract = "Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism",
pages = "778-770",
number = "7",
volume = "164",
doi = "10.1016/j.resmic.2013.03.002"
}

Stanić, M., Zakrzewska, J., Hadzibrahimović, M. R., Žižić, M., Marković, Z. S., Vučinić, Ž.,& Zivić, M.. (2013). Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology
Elsevier, Amsterdam., 164(7), 770-778.
https://doi.org/10.1016/j.resmic.2013.03.002

Stanić M, Zakrzewska J, Hadzibrahimović MR, Žižić M, Marković ZS, Vučinić Ž, Zivić M. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology. 2013;164(7):770-778.
doi:10.1016/j.resmic.2013.03.002 .

Stanić, Marina, Zakrzewska, Joanna, Hadzibrahimović, Mirzeta R, Žižić, Milan, Marković, Zoran S, Vučinić, Željko, Zivić, Miroslav, "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism" in Research in Microbiology, 164, no. 7 (2013):770-778,
https://doi.org/10.1016/j.resmic.2013.03.002 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/632
AB  - The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects
EP  - 69
IS  - 1
SP  - 61
VL  - 164
DO  - 10.1016/j.resmic.2012.08.007
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Spasojević, Ivan and Bogdanović Pristov, Jelena and Stanić, Marina and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2013",
abstract = "The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects",
pages = "69-61",
number = "1",
volume = "164",
doi = "10.1016/j.resmic.2012.08.007"
}

Žižić, M., Zivić, M., Spasojević, I., Bogdanović Pristov, J., Stanić, M., Cvetić-Antić, T.,& Zakrzewska, J.. (2013). The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 164(1), 61-69.
https://doi.org/10.1016/j.resmic.2012.08.007

Žižić M, Zivić M, Spasojević I, Bogdanović Pristov J, Stanić M, Cvetić-Antić T, Zakrzewska J. The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology. 2013;164(1):61-69.
doi:10.1016/j.resmic.2012.08.007 .

Žižić, Milan, Zivić, Miroslav, Spasojević, Ivan, Bogdanović Pristov, Jelena, Stanić, Marina, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects" in Research in Microbiology, 164, no. 1 (2013):61-69,
https://doi.org/10.1016/j.resmic.2012.08.007 . .

TY  - GEN
AU  - Stanić, Marina
AU  - Zivić, Miroslav
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/497
AB  - Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus
EP  - 186
SP  - 186
VL  - 40
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_497
ER  - 

@misc{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta R and Žižić, Milan and Zakrzewska, Joanna",
year = "2011",
abstract = "Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus",
pages = "186-186",
volume = "40",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_497"
}

Stanić, M., Zivić, M., Hadzibrahimović, M. R., Žižić, M.,& Zakrzewska, J.. (2011). Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters
Springer, New York., 40, 186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497

Stanić M, Zivić M, Hadzibrahimović MR, Žižić M, Zakrzewska J. Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters. 2011;40:186-186.
https://hdl.handle.net/21.15107/rcub_rimsi_497 .

Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta R, Žižić, Milan, Zakrzewska, Joanna, "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus" in European Biophysics Journal With Biophysics Letters, 40 (2011):186-186,
https://hdl.handle.net/21.15107/rcub_rimsi_497 .

TY  - JOUR
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Cvetic, T.
AU  - Živanović, Branka  D.
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/370
AB  - Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - Alternative respiration of fungus Phycomyces blakesleeanus
EP  - 217
IS  - 3
SP  - 207
VL  - 95
DO  - 10.1007/s10482-008-9304-5
ER  - 

@article{
author = "Zivić, Miroslav and Zakrzewska, Joanna and Stanić, Marina and Cvetic, T. and Živanović, Branka  D.",
year = "2009",
abstract = "Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "Alternative respiration of fungus Phycomyces blakesleeanus",
pages = "217-207",
number = "3",
volume = "95",
doi = "10.1007/s10482-008-9304-5"
}

Zivić, M., Zakrzewska, J., Stanić, M., Cvetic, T.,& Živanović, Branka  D.. (2009). Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 95(3), 207-217.
https://doi.org/10.1007/s10482-008-9304-5

Zivić M, Zakrzewska J, Stanić M, Cvetic T, Živanović, Branka  D.. Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2009;95(3):207-217.
doi:10.1007/s10482-008-9304-5 .

Zivić, Miroslav, Zakrzewska, Joanna, Stanić, Marina, Cvetic, T., Živanović, Branka  D., "Alternative respiration of fungus Phycomyces blakesleeanus" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 95, no. 3 (2009):207-217,
https://doi.org/10.1007/s10482-008-9304-5 . .

TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Žižić, Milan
AU  - Zivić, Miroslav
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/139
AB  - The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy
EP  - 486
SP  - 482
VL  - 1048
DO  - 10.1196/annals.1342.073
ER  - 

@article{
author = "Zakrzewska, Joanna and Žižić, Milan and Zivić, Miroslav",
year = "2005",
abstract = "The effect of anoxia and the respiratory chain inhibitors azide and cyanide on the polyphosphate content of Phycomyces was studied by in vivo (31)p NMR spectroscopy. Anoxia was manifested by a decrease of core polyphosphates (PPi) and increase of intracellular inorganic phosphate (P-i) signal. Normalized changes in PPi/P-i ratio between control and nitrogen-purged mycelia suggest that the sensitivity to anoxia differs with growth phases. Azide acts in the same way as anoxia, by decreasing the PPi/P-i ratio, while cyanide causes an increase of the PPi/P-i ratio.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy",
pages = "486-482",
volume = "1048",
doi = "10.1196/annals.1342.073"
}

Zakrzewska, J., Žižić, M.,& Zivić, M.. (2005). The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 482-486.
https://doi.org/10.1196/annals.1342.073

Zakrzewska J, Žižić M, Zivić M. The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:482-486.
doi:10.1196/annals.1342.073 .

Zakrzewska, Joanna, Žižić, Milan, Zivić, Miroslav, "The effect of anoxia on PolyP content of Phycomyces blakesleeanus mycelium studied by P-31 NMR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):482-486,
https://doi.org/10.1196/annals.1342.073 . .